Objective To investigate the effect of H2 S on lower limb ischemia-reperfusion(LIR) induced lung injury and explore the underlying mechanism. Methods Wistar rats were randomly divided into control group, IR group, IR+...Objective To investigate the effect of H2 S on lower limb ischemia-reperfusion(LIR) induced lung injury and explore the underlying mechanism. Methods Wistar rats were randomly divided into control group, IR group, IR+ Sodium Hydrosulphide(NaHS) group and IR+ DL-propargylglycine(PPG) group. IR group as lung injury model induced by LIR were given 4 h reperfusion following 4 h ischemia of bilateral hindlimbs with rubber bands. NaHS(0.78 mg/kg) as exogenous H2 S donor and PPG(60 mg/kg) which can suppress endogenous H2 S production were administrated before LIR, respectively. The lungs were removed for histologic analysis, the determination of wet-to-dry weight ratios and the measurement of mRNA and protein levels of aquaporin-1(AQP1), aquaporin-5(AQP5) as indexes of water transport abnormality, and mRNA and protein levels of Toll-like receptor 4(TLR4), myeloid differentiation primary-response gene 88(MyD88) and p-NF-κB as indexes of inflammation. Results LIR induced lung injury was accompanied with upregulation of TLR4-Myd88-NF-κB pathway and downregulation of AQP1/AQP5. NaHS pre-treatment reduced lung injury with increasing AQP1/AQP5 expression and inhibition of TLR4-Myd88-NF-κB pathway, but PPG adjusted AQP1/AQP5 and TLR4 pathway to the opposite side and exacerbated lung injury. Conclusion Endogenous H2 S, TLR4-Myd88-NF-κB pathway and AQP1/AQP5 were involved in LIR induced lung injury. Increased H2 S would alleviate lung injury and the effect is at least partially depend on the adjustment of TLR4-Myd88-NF-κB pathway and AQP1/AQP5 expression to reduce inflammatory reaction and lessen pulmonary edema.展开更多
BACKGROUND The efficacy and safety of transarterial chemoembolization(TACE)combined with lenvatinib plus programmed cell death protein-1(PD-1)for unresectable hepato-cellular carcinoma(HCC)have rarely been evaluated a...BACKGROUND The efficacy and safety of transarterial chemoembolization(TACE)combined with lenvatinib plus programmed cell death protein-1(PD-1)for unresectable hepato-cellular carcinoma(HCC)have rarely been evaluated and it is unknown which factors are related to efficacy.AIM To evaluate the efficacy and independent predictive factors of TACE combined with lenvatinib plus PD-1 inhibitors for unresectable HCC.METHODS This study retrospectively enrolled patients with unresectable HCC who received TACE/lenvatinib/PD-1 treatment between March 2019 and April 2022.Overall survival(OS)and progression-free survival(PFS)were determined.The objective response rate(ORR)and disease control rate(DCR)were evaluated in accordance with the modified Response Evaluation Criteria in Solid Tumors.Additionally,the prognostic factors affecting the clinical outcome were assessed.RESULTS One hundred and two patients were enrolled with a median follow-up duration of 12.63 months.The median OS was 26.43 months(95%CI:17.00-35.87),and the median PFS was 10.07 months(95%CI:8.50-11.65).The ORR and DCR were 61.76%and 81.37%,respectively.The patients with Barcelona Clinic Liver Cancer Classification(BCLC)B stage,early neutrophil-to-lymphocyte ratio(NLR)response(decrease),or early alpha-fetoprotein(AFP)response(decrease>20%)had superior OS and PFS than their counterparts.CONCLUSION This study showed that TACE/lenvatinib/PD-1 treatment was well tolerated with encouraging efficacy in patients with unresectable HCC.The patients with BCLC B-stage disease with early NLR response(decrease)and early AFP response(decrease>20%)may achieve better clinical outcomes with this triple therapy.展开更多
Background:Due to media opacity,it is usually difficult to accurately evaluate the postoperative visual acuity in cataracts patients.As a small and portable tool,the critical flicker fusion frequency(CFF)device reflec...Background:Due to media opacity,it is usually difficult to accurately evaluate the postoperative visual acuity in cataracts patients.As a small and portable tool,the critical flicker fusion frequency(CFF)device reflects the temporal resolution of visual function and has been widely used in clinical research.However,poor understanding of the technique and equipment limitations have restricted its clinical application in China.Main text:There was a decrease in the CFF value in various ophthalmic diseases,indicating that the CFF is sensitive to detect visual functional changes.A number of studies have shown that the CFF test can accurately distinguish patients with simple cataracts from those with cataracts combined with fundus disease,and,as a visual test,it can more accurately predict postoperative visual acuity without being affected by media opacity.This study comprehensive reviews the basic principles of CFF and its application in ophthalmology,especially in cataracts.Conclusions:As one of the tools for dynamic visual function detection,the CFF test could help doctors to assess the possible presence of fundus disease in cataracts patients,especially in eyes with dense cataracts,and more precisely provide a reasonable visual prognosis than other available visual tests.展开更多
Background:To evaluate the feature of different retinal layer segmentation in neuromyelitis optica spectrum disorders(NMOSD)with spectral-domain optical coherence tomography(SD-OCT)and to compare it with that in multi...Background:To evaluate the feature of different retinal layer segmentation in neuromyelitis optica spectrum disorders(NMOSD)with spectral-domain optical coherence tomography(SD-OCT)and to compare it with that in multiple sclerosis(MS),healthy controls(HC),and idiopathic optic neuritis(ION).Methods:We retrieved four electronic databases,including Pubmed,Embase,Cochrane Library,and Web of Science from inception to September 1st,2021.A meta-analysis was performed to compare different retinal layer segmentation thicknesses between patients with or without a history of optic neuritis(ON)in NMOSD and the control group,including patients with MS,HC,and ION.Results:Forty-two studies were included and the interval between the last ON onset and examination was greater than 3 months.Compared with that in HC eyes,the loss of retinal nerve fiber layer(RNFL)and macular ganglion cell and inner plexiform layer(GC-IPL)was serious in NMOSD eye especially after ON.Moreover,compared with that in ION eyes or MS-related-ON eyes,the injury to the peripapillary retinal nerve fiber layer(pRNFL)was severe in NMOSD-related-ON eyes.In addition,the correlation coefficient between pRNFL and prognostic visual acuity was 0.43.However,the one-arm study revealed the inner nuclear layer(INL)was thickened in NMOSDrelated-ON eyes compared with HC eyes.Conclusions:Inclusion of the RNFL and macular GC-IPL is recommended for monitoring disease progression and attention should be paid to changes in the INL.展开更多
Purpose:To investigate the application of critical flicker fusion frequency(CFF)in demyelinating optic neuritis(DON).Methods:A cross-sectional study.A total of 127 eyes in 69 DON patients and 63 eyes in 33 healthy con...Purpose:To investigate the application of critical flicker fusion frequency(CFF)in demyelinating optic neuritis(DON).Methods:A cross-sectional study.A total of 127 eyes in 69 DON patients and 63 eyes in 33 healthy control(HC)groups were included between January 2021 to September 2021 from Department of Ophthalmology,PLA General Hospital.Patients underwent best-corrected visual acuity(BCVA),visual field,optical coherence tomography(OCT),flash visual evoked potential(F-VEP),and CFF examinations.The affected eyes were divided into aquaporins 4(AQP4-),myelin oligodendrocyte glycoprotein(MOG-),and double negative DON according to serum antibody;mild,moderate,severe degree visual impairment according to BCVA0.5,0.1-0.5,<0.1;and 4 groups:<1,1~<3,3~<6 and>6 months according to time interval from onset to CFF examination.One-way ANOVA was used to perform above subgroup analysis.The correlations between CFF and F-VEP peak time,peak value,BCVA and mean visual filed defect(MD)were analyzed in order via Pearson correlation analysis.Results:he trichromatic values of red,green,and yellow in DON affected eyes were 21.83±9.03,23.66±10.21,24.09±10.77 Hz,respectively,which was significantly reduced compared with the HC group(t=-14.82,-14.22,-14.00;P<0.001).The subgroup analysis showed no significant difference between different antibody subtypes(P=0.914<0.848<0.604),whereas,a significant decrease of CFF trichromatic value was found in severe visual acuity impairment group(P<0.001).There was a significant difference in CFF-trichromatic values at different time points(P<0.001),to be specific,CFF fluctuated under 20Hz within 3 months after onset and tended to be stable around 24-28Hz.Correlation analysis showed that the peak time of F-VEP(r=-0.486,-0.515,-0.526;P<0.001),BVCA(r=-0.640,-0.659,-0.642;P<0.001),were negatively correlated with CFF trichromatic values,MD and CFF were positively correlated(r=0.486,0.453,0.476;P=0.003,0.006,0.004).Conclusions:A significant decrease of CFF value was found in DON-affected eyes,and it has a good correlation with BCVA,MD and latency of F-VEP,and can better reflect the impairment of visual function.展开更多
基金supported by the Military Health Care Foundation during the 12th Five-year Plan Period(11BZ21)the Military Scientific Research Foundation during the 12th Five-year Plan Period(BWS12J051)
文摘Objective To investigate the effect of H2 S on lower limb ischemia-reperfusion(LIR) induced lung injury and explore the underlying mechanism. Methods Wistar rats were randomly divided into control group, IR group, IR+ Sodium Hydrosulphide(NaHS) group and IR+ DL-propargylglycine(PPG) group. IR group as lung injury model induced by LIR were given 4 h reperfusion following 4 h ischemia of bilateral hindlimbs with rubber bands. NaHS(0.78 mg/kg) as exogenous H2 S donor and PPG(60 mg/kg) which can suppress endogenous H2 S production were administrated before LIR, respectively. The lungs were removed for histologic analysis, the determination of wet-to-dry weight ratios and the measurement of mRNA and protein levels of aquaporin-1(AQP1), aquaporin-5(AQP5) as indexes of water transport abnormality, and mRNA and protein levels of Toll-like receptor 4(TLR4), myeloid differentiation primary-response gene 88(MyD88) and p-NF-κB as indexes of inflammation. Results LIR induced lung injury was accompanied with upregulation of TLR4-Myd88-NF-κB pathway and downregulation of AQP1/AQP5. NaHS pre-treatment reduced lung injury with increasing AQP1/AQP5 expression and inhibition of TLR4-Myd88-NF-κB pathway, but PPG adjusted AQP1/AQP5 and TLR4 pathway to the opposite side and exacerbated lung injury. Conclusion Endogenous H2 S, TLR4-Myd88-NF-κB pathway and AQP1/AQP5 were involved in LIR induced lung injury. Increased H2 S would alleviate lung injury and the effect is at least partially depend on the adjustment of TLR4-Myd88-NF-κB pathway and AQP1/AQP5 expression to reduce inflammatory reaction and lessen pulmonary edema.
文摘BACKGROUND The efficacy and safety of transarterial chemoembolization(TACE)combined with lenvatinib plus programmed cell death protein-1(PD-1)for unresectable hepato-cellular carcinoma(HCC)have rarely been evaluated and it is unknown which factors are related to efficacy.AIM To evaluate the efficacy and independent predictive factors of TACE combined with lenvatinib plus PD-1 inhibitors for unresectable HCC.METHODS This study retrospectively enrolled patients with unresectable HCC who received TACE/lenvatinib/PD-1 treatment between March 2019 and April 2022.Overall survival(OS)and progression-free survival(PFS)were determined.The objective response rate(ORR)and disease control rate(DCR)were evaluated in accordance with the modified Response Evaluation Criteria in Solid Tumors.Additionally,the prognostic factors affecting the clinical outcome were assessed.RESULTS One hundred and two patients were enrolled with a median follow-up duration of 12.63 months.The median OS was 26.43 months(95%CI:17.00-35.87),and the median PFS was 10.07 months(95%CI:8.50-11.65).The ORR and DCR were 61.76%and 81.37%,respectively.The patients with Barcelona Clinic Liver Cancer Classification(BCLC)B stage,early neutrophil-to-lymphocyte ratio(NLR)response(decrease),or early alpha-fetoprotein(AFP)response(decrease>20%)had superior OS and PFS than their counterparts.CONCLUSION This study showed that TACE/lenvatinib/PD-1 treatment was well tolerated with encouraging efficacy in patients with unresectable HCC.The patients with BCLC B-stage disease with early NLR response(decrease)and early AFP response(decrease>20%)may achieve better clinical outcomes with this triple therapy.
基金the Program of National Natural Science Foundation of China(No.82070937)(No.81870640)National Natural Science Foundation for Young Scientists of China(No.82000923)(No.82101097).
文摘Background:Due to media opacity,it is usually difficult to accurately evaluate the postoperative visual acuity in cataracts patients.As a small and portable tool,the critical flicker fusion frequency(CFF)device reflects the temporal resolution of visual function and has been widely used in clinical research.However,poor understanding of the technique and equipment limitations have restricted its clinical application in China.Main text:There was a decrease in the CFF value in various ophthalmic diseases,indicating that the CFF is sensitive to detect visual functional changes.A number of studies have shown that the CFF test can accurately distinguish patients with simple cataracts from those with cataracts combined with fundus disease,and,as a visual test,it can more accurately predict postoperative visual acuity without being affected by media opacity.This study comprehensive reviews the basic principles of CFF and its application in ophthalmology,especially in cataracts.Conclusions:As one of the tools for dynamic visual function detection,the CFF test could help doctors to assess the possible presence of fundus disease in cataracts patients,especially in eyes with dense cataracts,and more precisely provide a reasonable visual prognosis than other available visual tests.
基金supported by China-USA intergovernmental Cooperation program(2018YFE0113900).
文摘Background:To evaluate the feature of different retinal layer segmentation in neuromyelitis optica spectrum disorders(NMOSD)with spectral-domain optical coherence tomography(SD-OCT)and to compare it with that in multiple sclerosis(MS),healthy controls(HC),and idiopathic optic neuritis(ION).Methods:We retrieved four electronic databases,including Pubmed,Embase,Cochrane Library,and Web of Science from inception to September 1st,2021.A meta-analysis was performed to compare different retinal layer segmentation thicknesses between patients with or without a history of optic neuritis(ON)in NMOSD and the control group,including patients with MS,HC,and ION.Results:Forty-two studies were included and the interval between the last ON onset and examination was greater than 3 months.Compared with that in HC eyes,the loss of retinal nerve fiber layer(RNFL)and macular ganglion cell and inner plexiform layer(GC-IPL)was serious in NMOSD eye especially after ON.Moreover,compared with that in ION eyes or MS-related-ON eyes,the injury to the peripapillary retinal nerve fiber layer(pRNFL)was severe in NMOSD-related-ON eyes.In addition,the correlation coefficient between pRNFL and prognostic visual acuity was 0.43.However,the one-arm study revealed the inner nuclear layer(INL)was thickened in NMOSDrelated-ON eyes compared with HC eyes.Conclusions:Inclusion of the RNFL and macular GC-IPL is recommended for monitoring disease progression and attention should be paid to changes in the INL.
基金National Key Research and Development Program(2018YFE0113900).
文摘Purpose:To investigate the application of critical flicker fusion frequency(CFF)in demyelinating optic neuritis(DON).Methods:A cross-sectional study.A total of 127 eyes in 69 DON patients and 63 eyes in 33 healthy control(HC)groups were included between January 2021 to September 2021 from Department of Ophthalmology,PLA General Hospital.Patients underwent best-corrected visual acuity(BCVA),visual field,optical coherence tomography(OCT),flash visual evoked potential(F-VEP),and CFF examinations.The affected eyes were divided into aquaporins 4(AQP4-),myelin oligodendrocyte glycoprotein(MOG-),and double negative DON according to serum antibody;mild,moderate,severe degree visual impairment according to BCVA0.5,0.1-0.5,<0.1;and 4 groups:<1,1~<3,3~<6 and>6 months according to time interval from onset to CFF examination.One-way ANOVA was used to perform above subgroup analysis.The correlations between CFF and F-VEP peak time,peak value,BCVA and mean visual filed defect(MD)were analyzed in order via Pearson correlation analysis.Results:he trichromatic values of red,green,and yellow in DON affected eyes were 21.83±9.03,23.66±10.21,24.09±10.77 Hz,respectively,which was significantly reduced compared with the HC group(t=-14.82,-14.22,-14.00;P<0.001).The subgroup analysis showed no significant difference between different antibody subtypes(P=0.914<0.848<0.604),whereas,a significant decrease of CFF trichromatic value was found in severe visual acuity impairment group(P<0.001).There was a significant difference in CFF-trichromatic values at different time points(P<0.001),to be specific,CFF fluctuated under 20Hz within 3 months after onset and tended to be stable around 24-28Hz.Correlation analysis showed that the peak time of F-VEP(r=-0.486,-0.515,-0.526;P<0.001),BVCA(r=-0.640,-0.659,-0.642;P<0.001),were negatively correlated with CFF trichromatic values,MD and CFF were positively correlated(r=0.486,0.453,0.476;P=0.003,0.006,0.004).Conclusions:A significant decrease of CFF value was found in DON-affected eyes,and it has a good correlation with BCVA,MD and latency of F-VEP,and can better reflect the impairment of visual function.
