Objective: To study the effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury (ALI) in mice. Methods: Kunming mice were selected as experimental animals and randomly divided in...Objective: To study the effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury (ALI) in mice. Methods: Kunming mice were selected as experimental animals and randomly divided into normal control group (NC group), acute lung injury group (ALI group) and n-butanol extract of Rumex gmelini Turcz group (Rum group). ALI group and Rum group were established into ALI models by intraperitoneal injection of endotoxin, and Rum group were given intragastric administration of n-butanol extract of Rumex gmelini Turcz for intervention before model establishment. 12 h after endotoxin injection, the superior lobe of right lung was taken to determine the water content, and the inferior lobe of right lung was taken to determine the contents of AQPs molecules, inflammatory response molecules and oxidative stress molecules. Results: 12 h after endotoxin injection, the water content of lung tissue in Rum group was (6.82±0.97)%. After variance analysis, the water content of lung tissue in ALI group was significantly higher than that in NC group, AQP1 and AQP5 protein levels in lung tissue were significantly lower than those of NC group, AQP3 and AQP4 protein levels were not different from those of NC group, and MPO, NF-kB, TNF-α, HMGB1, IL-8, ROS, ATP, MDA, Bax and Caspase-3 protein levels were significantly higher than those of NC group;the water content of lung tissue in Rum group was significantly lower than that in ALI group, AQP1 and AQP5 protein levels in lung tissue were significantly higher than those of ALI group, AQP3 and AQP4 protein levels were not different from those of ALI group, and MPO, NF-kB, TNF-α, HMGB1, IL-8, ROS, ATP, MDA, Bax and Caspase-3 protein levels were significantly lower than those of ALI group. Conclusion:The n-butanol extract of Rumex gmelini Turcz reduce the pulmonary edema, inflammatory response, oxidative stress and apoptosis during the endotoxin-induced ALI in mice.展开更多
Objective: To evaluate the systemic inflammatory and stress response in patients with COPD complicated by pulmonary infection after bronchoalveolar lavage with ambroxol hydrochloride. Methods: A total of 78 patients w...Objective: To evaluate the systemic inflammatory and stress response in patients with COPD complicated by pulmonary infection after bronchoalveolar lavage with ambroxol hydrochloride. Methods: A total of 78 patients with COPD complicated by acute pulmonary infection who were treated in the hospital between October 2015 and January 2017 were collected as research subjects and divided into control group (n=39) and study group (n=39) by random number table. Control group received routine treatment, and study group received the routine + bronchoalveolar lavage with ambroxol hydrochloride. The differences in serum levels of inflammatory factors and oxidative stress indexes were compared between the two groups before treatment and after 1 week of treatment. Results: There was no statistically significant difference in serum levels of inflammatory factors and oxidative stress indexes between the two groups before treatment. After 1 week of treatment, serum inflammatory factors IL-2, IL-6, IL-8, TNF- , CRP and PCT levels of study group were lower than those of control group;serum oxidation indexes MDA, MPO and AOPPs levels were lower than those of control group while anti-oxidation indexes SOD, T-AOC and GSH-Px levels were higher than those of control group. Conclusion: Bronchoalveolar lavage with ambroxol hydrochloride can significantly inhibit the systemic inflammatory and oxidative stress response and help control the disease in patients with COPD complicated by pulmonary infection.展开更多
文摘Objective: To study the effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury (ALI) in mice. Methods: Kunming mice were selected as experimental animals and randomly divided into normal control group (NC group), acute lung injury group (ALI group) and n-butanol extract of Rumex gmelini Turcz group (Rum group). ALI group and Rum group were established into ALI models by intraperitoneal injection of endotoxin, and Rum group were given intragastric administration of n-butanol extract of Rumex gmelini Turcz for intervention before model establishment. 12 h after endotoxin injection, the superior lobe of right lung was taken to determine the water content, and the inferior lobe of right lung was taken to determine the contents of AQPs molecules, inflammatory response molecules and oxidative stress molecules. Results: 12 h after endotoxin injection, the water content of lung tissue in Rum group was (6.82±0.97)%. After variance analysis, the water content of lung tissue in ALI group was significantly higher than that in NC group, AQP1 and AQP5 protein levels in lung tissue were significantly lower than those of NC group, AQP3 and AQP4 protein levels were not different from those of NC group, and MPO, NF-kB, TNF-α, HMGB1, IL-8, ROS, ATP, MDA, Bax and Caspase-3 protein levels were significantly higher than those of NC group;the water content of lung tissue in Rum group was significantly lower than that in ALI group, AQP1 and AQP5 protein levels in lung tissue were significantly higher than those of ALI group, AQP3 and AQP4 protein levels were not different from those of ALI group, and MPO, NF-kB, TNF-α, HMGB1, IL-8, ROS, ATP, MDA, Bax and Caspase-3 protein levels were significantly lower than those of ALI group. Conclusion:The n-butanol extract of Rumex gmelini Turcz reduce the pulmonary edema, inflammatory response, oxidative stress and apoptosis during the endotoxin-induced ALI in mice.
文摘Objective: To evaluate the systemic inflammatory and stress response in patients with COPD complicated by pulmonary infection after bronchoalveolar lavage with ambroxol hydrochloride. Methods: A total of 78 patients with COPD complicated by acute pulmonary infection who were treated in the hospital between October 2015 and January 2017 were collected as research subjects and divided into control group (n=39) and study group (n=39) by random number table. Control group received routine treatment, and study group received the routine + bronchoalveolar lavage with ambroxol hydrochloride. The differences in serum levels of inflammatory factors and oxidative stress indexes were compared between the two groups before treatment and after 1 week of treatment. Results: There was no statistically significant difference in serum levels of inflammatory factors and oxidative stress indexes between the two groups before treatment. After 1 week of treatment, serum inflammatory factors IL-2, IL-6, IL-8, TNF- , CRP and PCT levels of study group were lower than those of control group;serum oxidation indexes MDA, MPO and AOPPs levels were lower than those of control group while anti-oxidation indexes SOD, T-AOC and GSH-Px levels were higher than those of control group. Conclusion: Bronchoalveolar lavage with ambroxol hydrochloride can significantly inhibit the systemic inflammatory and oxidative stress response and help control the disease in patients with COPD complicated by pulmonary infection.
