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Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa brevifl ora Desert Steppe 被引量:5
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作者 HU Hong-lian LIU Yong-zhi +2 位作者 LI Ya-kui LU De-xun GAO Min 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第5期1065-1072,共8页
The application of n-alkane as markers to estimate herbage intake, apparent digestibility and species composition of diet consumed by grazing sheep was studied. Six local Mongolian sheep were used to determine dry mat... The application of n-alkane as markers to estimate herbage intake, apparent digestibility and species composition of diet consumed by grazing sheep was studied. Six local Mongolian sheep were used to determine dry matter (DM) intake, apparent DM digestibility and species composition of diet during summer, autumn and winter. Animals were orally dosed twice daily with n-alkane gelatin capsules containing 60 mg C32-alkane as an external marker. Diet composition was estimated by comparing the odd-chain n-alkanes pattern profile (C27-C31) of the consumed plant species with the n-alkanes fecal concentrations of grazing animals, using a non-negative least squares algorithm called EATWHAT software package. The alkane pair C32:C33 and C33 alkane were used to estimate DM intake and diet apparent DM digestibility, respectively. The results showed that daily dry matter intake of the sheep were 1.77, 1.61 and 1.18 kg d-1 in summer, autumn and winter, respectively. Apparent DM digestibility, crude protein (CP), metabolizable energy (ME) and neutral detergent fiber (NDF) intake of diet consumed by sheep decreased significantly (P〈0.01) from summer to winter, with no evident changes in ADF and ADL intake. Diet composition indicated Artemisia frigida Willd was the most dominant diet component, contributed 79.68, 68.12 and 86.26% of sheep's diets in summer, autumn and winter, respectively. Cleistogenes songorica Ohwi and Convolvulus arnmannii Desr were the important components of the diet. Although Stipa breviflora Griseb is one of the main plant species in the study area, the sheep rarely choosed it. The study indicated that CP and ME in diet consumed by sheep were deficient in winter. Therefore, appropriate supplementation strategies should be indispensable during this period. 展开更多
关键词 N-ALKANES diet composition INTAKE apparent digestibility SHEEP
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Molecular Clone, Expression, and Prediction of Construction and Function to Key Genes of Interleukin Family of Porcine 被引量:4
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作者 JING Zhi-zhong DOU Yong-xi +5 位作者 LUO Qi-hui CHEN Guo-hua MENG Xue-lian ZHENG Ya-dong LUO Xue-nong CAI Xue-peng 《Agricultural Sciences in China》 CAS CSCD 2007年第7期869-876,共8页
This research was to clone, express, and analyze the structure and function of major molecules of porcine interleukin family. Genes of porcine interleukin family were cloned by RT-PCR from stimulated porcine PBMC by L... This research was to clone, express, and analyze the structure and function of major molecules of porcine interleukin family. Genes of porcine interleukin family were cloned by RT-PCR from stimulated porcine PBMC by LPS and PHA, and then expressed in E. coli, and the structure and function of these molecules were predicted by ExPASY. The results showed that genes of IL-4, IL-6, and IL-18 were successfully cloned and expressed. Furthermore, the expression products of recombinant IL-4 and IL-6 both have multiple biological activities. By analyzing these genes with the NCBI/GenBank data, the homologies of the nucleotide acid sequence are 99.25, 99.21, and 100%, respectively, and have great species differences when compared with other animal species. The results of the prediction showed that all these molecules contain several phosphorylation, glycosylation, protein kinase, and signal transduction bonding sites in secondary structure, and all are compact globularity protein in space configuration. These characteristics of structure are the basis for their multiple biological functions. The genes, structure and function of key molecular of porcine interleukin family were successfully cloned, expressed, and analyzed in this paper. 展开更多
关键词 porcine interleukin gene family analysis of homologies prediction of construction
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Determination of reference intervals for metabolic profile of Hanwoo cows at early, middle and late gestation periods 被引量:1
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作者 Da Chuan Piao Tao Wang +4 位作者 Jae Sung Lee Renato SA Vega Sang Ki Kang Yun Jaie Choi Hong Gu Lee 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2015年第2期252-256,共5页
Background: Metabolic profile was initially designed as a presymptomatic diagnostic aid based on statistical analyses of blood metabolites to provide an early warning of certain types of metabolic disorder. However, ... Background: Metabolic profile was initially designed as a presymptomatic diagnostic aid based on statistical analyses of blood metabolites to provide an early warning of certain types of metabolic disorder. However, there is little metabolic profile data available about Korean Hanwoo cows. Therefore, this study aimed to determine the reference intervals of metabolic profile for Korean Hanwoo cows. Methods: Healthy animals (2,205) were selected and divided into early (day 1 to 95), middle (day 96 to 190) and late (day 191 to 285) period according to their gestating period. Metabolic profile including total protein (TP), albumin (AIb), urea (UREA), glucose (Glu), total cholesterol (T-Cho), long-chain fatty acid (LCFA), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), creatinine (Crea), calcium (Ca), inorganic phosphorous (iP) and magnesium (Mg) were analyzed using a TBA-4OFR automatic biochemical analyzer. The data of Korean Hanwoo cows were then compared to those of the Japanese Wagyu cows. Results: Most of the data of the Korean Hanwoo cows were relatively higher than those of Japanese Wagyu cows, with the exception of Glu and GGT. This may indicate that the nutritional level of feed for the Korean Hanwoo cows was higher than that of the Japanese Wagyu cows because of the different feeding system. In particular, relatively higher levels of UREA and LCFA were observed in the Korean Hanwoo cows, and this may also contribute to the low reproduction efficiency. Conclusions: These findings may provide some theoretical basis for understanding the reproductive and feeding situation of Korean Hanwoo cows. 展开更多
关键词 Hanwoo cows Metabolic profile Reference intervals Wagyu cows
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Effects of frozen storage on texture,chemical quality indices and sensory properties of crisp Nile tilapia fillets 被引量:1
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作者 Xi Xie Xiaoqian Zhai +5 位作者 Meiyan Chen Qingqing Li Yao Huang Lijuan Zhao Qin Wang Li Lin 《Aquaculture and Fisheries》 CSCD 2023年第6期626-633,共8页
Crisp Nile tilapia(Oreochromis niloticus)is a kind of valuable fish product with high muscle firmness and crispiness texture.However,with the change of physicochemical in crisp Nile tilapia,the frozen storage paramete... Crisp Nile tilapia(Oreochromis niloticus)is a kind of valuable fish product with high muscle firmness and crispiness texture.However,with the change of physicochemical in crisp Nile tilapia,the frozen storage parameters and quality would be different comparing to normal Nile tilapia.Thus,the aim of this study was to analyze the changes in texture,chemical quality indices and volatile compounds of Nile tilapia fillets during frozen storage.The remaining storage time of the crisp Nile tilapia fillets could be estimated within 120 days.During frozen storage,fillets resulted in softer started at 90-day,and 36.75%,45.74%,48.81%and 20.37%reduction of hardness,springiness,gumminess and chewiness were observed at 120-day.Thiobarbituric acid reactive substances(TBARS)for frozen samples showed similar with fresh fillets within 60 days,while the TBARS was 1.97 folds higher than fresh one at 120-day.Low-field nuclear magnetic resonance(LF-NMR)indicated that the water loss of Nile tilapia fillets was significant changed at 120-day,which reduced more than 12.5%water out of weight.The volatile compound analysis showed that more free fatty acid will be detected at 120-day comparing to the fresh fillets.The combined results demonstrated that the crisp Nile tilapia fillets had the best quality before 60 days frozen storage then loss of some quality properties in longer freezing.Thus,these results identified the ideal storage strategy for the preservation of crisp Nile tilapia without affecting sensory appeal and commercial value. 展开更多
关键词 Crisp Nile tilapia Frozen storage TEXTURE QUALITY Shelf life
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Recent advances on the circadian gene PER2 and metabolic rhythm of lactation of mammary gland 被引量:2
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作者 Mengzhi Wang Yujia Jing +3 位作者 Liangyu Hu Jian Gao Luyang Ding Jun Zhang 《Animal Nutrition》 SCIE 2015年第4期257-261,共5页
Due to regulation by circadian rhythm, the lactation of the mammary gland has rhythmicity. As one of prominent members of period protein family which regulates biological rhythms, PER2 plays an important role in devel... Due to regulation by circadian rhythm, the lactation of the mammary gland has rhythmicity. As one of prominent members of period protein family which regulates biological rhythms, PER2 plays an important role in developing the milk duct and maintaining the polarity and the morphology of the mammary epithelium; at the same time, it is also closely related with the metabolism of milk protein and milk fat. This paper summarized recent researches on PER2 gene and related researches on mammary gland development and metabolism to provide some information for the studies of the theory and technology on physiological functions of the mammary gland and milk quality control. 展开更多
关键词 Circadian gene PER2 Mammary gland Lactation metabolism
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Construction of an infectious cDNA clone of Tembusu virus isolated from breeder Peking ducks
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作者 Hao Chen Ying Zhang +2 位作者 Xin Zhang Jinfeng Ti Youxiang Diao 《Virologica Sinica》 SCIE CAS CSCD 2016年第3期262-265,共4页
Dear Editor,Since April 2010,an outbreak of a new disease has elicited symptoms of high fever,loss of appetite,and reduction in egg production in layer ducks in eastern China;this phenomenon has now spread throughout ... Dear Editor,Since April 2010,an outbreak of a new disease has elicited symptoms of high fever,loss of appetite,and reduction in egg production in layer ducks in eastern China;this phenomenon has now spread throughout China(Cao et al.,2011;Su et al.,2011).The causative agent of the disease was identified as Tembusu virus(TMUV),which was classified into the genus Flavivirus, 展开更多
关键词 clone infectious Peking classified eastern parental sequenced throughout plasmid genomic
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