Targeted delivery of anti-tumor drugs and overcoming drug resistance in malig-nant tumor cells remain significant clinical challenges.However,there are only few effective methods to address these issues.Extracellular ...Targeted delivery of anti-tumor drugs and overcoming drug resistance in malig-nant tumor cells remain significant clinical challenges.However,there are only few effective methods to address these issues.Extracellular vesicles(EVs),actively secreted by cells,play a crucial role in intercellular information transmis-sion and cargo transportation.Recent studies have demonstrated that engineered EVs can serve as drug delivery carriers and showed promising application prospects.Nevertheless,there is an urgent need for further improvements in the isolation and purification of EVs,surface modification techniques,drug assem-bly processes,and precise recognition of tumor cells for targeted drug delivery purposes.In this review,we summarize the applications of engineered EVs in cancer treatment and overcoming drug resistance,and current challenges asso-ciated with engineered EVs are also discussed.This review aims to provide new insights and potential directions for utilizing engineered EVs as targeted delivery systems for anti-tumor drugs and overcoming drug resistance in the near future.展开更多
Glycoprotein from Ginkgo biloba kernel may be allergic. In this paper, the allergic proteins were identified with Western blotting, and the 32 kDa glycoprotein was purified with ion exchange chromatography and gel chr...Glycoprotein from Ginkgo biloba kernel may be allergic. In this paper, the allergic proteins were identified with Western blotting, and the 32 kDa glycoprotein was purified with ion exchange chromatography and gel chromatography. With Western blotting, there were 3 allergic proteins with molecular weight 21, 32, and 36 kDa. With SDS-PAGE analysis and measurements of the protein and sugar contents, the isolation and purification technology of 32 kDa was confirmed. Ginkgo crude protein extract was precipitated with ammonium sulphate (saturation gradient: 40-80%). The precipitate was purified by chromatography with DEAE-cellulose 52, then chromatography with Sephadex G-200 and the target glycoprotein was finally obtained. The analysis results showed the molecule of the glycoprotein was 32.12 kDa and the ratio of protein to sugar was 20.56:1. In conclusion, the purification method could be used in preparation of the glycoprotein, and the study could provide a basis for the further research of the glycoprotein.展开更多
A new coordination polymer [Cu2(OH)(nbta)(tib)(H2 O)2]n(1, H3 nbta = 5-nitro-1,2,3-benzenetricarboxylic acid, tib = 1-(4 H-1,2,4-triazol-4-yl)-4-(imidazol-1-yl)benzene) constructed from dimeric Cu(Ⅱ) units has been s...A new coordination polymer [Cu2(OH)(nbta)(tib)(H2 O)2]n(1, H3 nbta = 5-nitro-1,2,3-benzenetricarboxylic acid, tib = 1-(4 H-1,2,4-triazol-4-yl)-4-(imidazol-1-yl)benzene) constructed from dimeric Cu(Ⅱ) units has been synthesized under hydrothermal conditions and structurally characterized by single-crystal X-ray diffraction analysis. The compound crystallizes in monoclinic system, space group I2/c, with a = 17.8474(9), b = 11.7588(6), c = 21.9221(11) ?, β = 104.419(5)°, V = 4455.7(4) ?3 and Z = 8. Compound 1 is a three-dimensional structure constructed from dimeric Cu(Ⅱ) units and presents a 2-nodal(3,5)-connected net. Moreover, the thermal stability and solid UV-Vis absorption spectra have been investigated.展开更多
The cyanide groups of letrozole(1-[bis(4-cyanophenyl)methyl]-1,2,4-triazole) were hydrolyzed into the carboxylic groups under alkaline conditions. Then the hydrolysis product(1-[bis(4-carboxylphenyl)methyl]-1,2,4-tria...The cyanide groups of letrozole(1-[bis(4-cyanophenyl)methyl]-1,2,4-triazole) were hydrolyzed into the carboxylic groups under alkaline conditions. Then the hydrolysis product(1-[bis(4-carboxylphenyl)methyl]-1,2,4-triazole, H2ltzda) reacted with Co(NO3)2·6H2O in the presence of different bipyridl-typed ligands under hydrothermal conditions. As a result, two new Co(Ⅱ) MOFs were obtained, formulated as [Co2(ltzda)2(bpp)2]n(1) and {[Co2(ltzda)2(bpy)]·4H2O}n(2)(bpp = 1,3-di(4-pyridyl)propane, bpy = 4,4’-bipyridine). Two MOFs have been characterized by single-crystal X-ray diffraction, thermogravimetric analysis and magnetic measure. 1 displays a 2D metal-organic framework containing wave layers and 2 shows a pillared-layer structure existing in a 2-fold interpenetrated mode.展开更多
A cobalt coordination polymer, {[Co_2(tdc)_2(bimm)_2]·(3 DMF)}n, was synthesized based on 2,5-thiophenedicarboxylic acid(H2 tdc) and bis(imidazol-1-yl)methane(bimm) mixed ligands. The asymmetric unit of the compl...A cobalt coordination polymer, {[Co_2(tdc)_2(bimm)_2]·(3 DMF)}n, was synthesized based on 2,5-thiophenedicarboxylic acid(H2 tdc) and bis(imidazol-1-yl)methane(bimm) mixed ligands. The asymmetric unit of the complex contains two Co^(^(2+)) cations, two tdc2-dianions, two neutral bimm ligands, and three free DMF molecules. In the complex, deprotonated tdc2-dianions alternately bridge the adjacent Co^(2+) cations to generate chains, which are further connected by the flexible bimm ligands to form a 3 D structure containing adamantanoid-like subunits. In topology, the structure of 1 represents a 4-connected uninodal two-fold interpenetrated dia(66) topology. Moreover, the solid UV-Vis absorption spectra of the complex have also been investigated.展开更多
One two-dimensional coordination polymer with a formula of {[Cd(L)(imidazole)(H_2O)](H_2O)}n(1) was obtained by the synthetic reactions in aqueous solution using a newly synthesized H_2L(H_2L = methyl-3-hydroxy-5-carb...One two-dimensional coordination polymer with a formula of {[Cd(L)(imidazole)(H_2O)](H_2O)}n(1) was obtained by the synthetic reactions in aqueous solution using a newly synthesized H_2L(H_2L = methyl-3-hydroxy-5-carboxy-2-thiophenecarboxylate) ligand.Compound1 crystallizes in monoclinic system,space group C2/c with a = 18.3176(11),b = 8.5366(9),c =8.4152(5) ?,β = 101.789(6)o,V = 2797.1(3) ?~3,D_c = 1.979 g/cm^3,C_(10)H_(12)N_2O_7SCd,Mr = 416.68 F(000) = 1648,μ = 1.745 mm^(-1),F(000) = 1648,the final R = 0.0323 and wR = 0.0604 for 2604 observed reflections with I > 2s(I).Structure analyses reveal that the compound is constructed by dinuclear Cd(Ⅱ) clusters bridged by two hydroxyl oxygens of L^(2-)anions,which features a two-dimensional network with 4-connected sql topology.Furthermore,the compound exhibits high thermal stability and intense fluorescent emission,and could be explored for potentia luminescent materials.展开更多
An amphiphilic AIE photosensitizer has been successfully developed,which allows for easily inserting into the bacterial membranes.Binding experiments with phospholipid preliminary demonstrates its membrane specificity...An amphiphilic AIE photosensitizer has been successfully developed,which allows for easily inserting into the bacterial membranes.Binding experiments with phospholipid preliminary demonstrates its membrane specificity.As expected,it is proved to possess a broad-spectrum bacterial staining performance and photodynamic antibacterial activity toward S.aureus and E.coli.展开更多
Based on 5-dimethylamino-isophthalic acid(H2dia)and 1,2-bis(4-pyridyl)ethylene(bpe)mixed linkers,a new d10-configuration Zn(Ⅱ)coordination polymer material,[Zn(dia)(bpe)(H2O)]n(1),was synthesized.In the asymmetric un...Based on 5-dimethylamino-isophthalic acid(H2dia)and 1,2-bis(4-pyridyl)ethylene(bpe)mixed linkers,a new d10-configuration Zn(Ⅱ)coordination polymer material,[Zn(dia)(bpe)(H2O)]n(1),was synthesized.In the asymmetric unit,there are one Zn2+ion,two dia2-anions,one neutral bpe linker and one free H2O molecule.In the complex,adjacent Zn2+ions are alternately bridged by the dia2-dianions to generate chains which are further connected by flexible bpe ligands to form a two-dimensional(2 D)layer structure.In topology,the structure of 1 represents a non-interpenetrating 4-connected sql topology.Moreover,infrared spectroscopy,elemental analyses,thermogravimetric analysis,powder X-ray diffraction,solid UV-Vis absorption spectra and photoluminescent property of 1 are also investigated.展开更多
Although porphyrin-based metal-organic frameworks(MOFs)have been widely explored as photosensitizers for photodynamic therapy,how the size will affect the light-induced catalytic activity for generation of reactive ox...Although porphyrin-based metal-organic frameworks(MOFs)have been widely explored as photosensitizers for photodynamic therapy,how the size will affect the light-induced catalytic activity for generation of reactive oxygen species(ROS)still remain unclear.Herein,we first report the size-controlled synthesis of two-dimensional(2D)porphyrin-based PCN-134 MOF nanosheets by a two-step solvothermal method to explore the size effect on its PDT performance,thus yielding enhanced photodynamic antimicrobial therapy.By simply controlling the reaction temperature in the synthesis process,the bulk PCN-134 crystal,large PCN-134(L-PCN-134)nanosheets with a lateral size of 2–3μm and thickness of 33.2–37.5 nm and small PCN-134 nanosheets(S-PCN-134)with a lateral size of 160–180 nm and thickness of 9.1–9.7 nm were successfully prepared.Interestingly,the S-PCN-134 nanosheets exhibit much higher photodynamic activity for ROS generation than that of the bulk 3D PCN-134 crystal and L-PCN-134 nanosheets under a660 nm laser irradiation,suggesting that the photodynamic activity of PCN-134 MOF increases when the size reduces.Therefore,the S-PCN-134 nanosheets show much enhanced performance when used as a photosensitizer for photodynamic antimicrobial activity and wound healing.展开更多
Three novel dithienylethenes modified by bifluoroboron β-diketonate fragments have been successfully developed. Upon blue light irradiation, they reached photostationary state within 2-5 s, as well as 100% conversion...Three novel dithienylethenes modified by bifluoroboron β-diketonate fragments have been successfully developed. Upon blue light irradiation, they reached photostationary state within 2-5 s, as well as 100% conversion ratio and photocyclization quantum yield of > 0.70. Such fascinating photochromism were endowed by collaborative role of electron-withdrawing effect of BF_(2)bdk group to reduce HOMO-LUMO electronic gap for the open isomer, together with intramolecular hydrogen bonds and CH-π interactions favoring antiparallel conformation fixation. Moreover, they displayed specific discrimination and photoswitchable bacterial imaging for S. aureus.展开更多
基金This work was supported by grants from the National Natural Science Foundation of China(No.81972394 and 82272856)the Guang Dong Basic and Applied Basic Research Foundation(No.2022A1515010437)+1 种基金the President Foundation of The Third Affiliated Hospital of Southern Medical University(No.YM2021010,No.YM2021011)the Scientific and Technological Support Project of Guizhou Province(Qian Ke He Zhi Cheng 2023]Yi Ban 262)。
文摘Targeted delivery of anti-tumor drugs and overcoming drug resistance in malig-nant tumor cells remain significant clinical challenges.However,there are only few effective methods to address these issues.Extracellular vesicles(EVs),actively secreted by cells,play a crucial role in intercellular information transmis-sion and cargo transportation.Recent studies have demonstrated that engineered EVs can serve as drug delivery carriers and showed promising application prospects.Nevertheless,there is an urgent need for further improvements in the isolation and purification of EVs,surface modification techniques,drug assem-bly processes,and precise recognition of tumor cells for targeted drug delivery purposes.In this review,we summarize the applications of engineered EVs in cancer treatment and overcoming drug resistance,and current challenges asso-ciated with engineered EVs are also discussed.This review aims to provide new insights and potential directions for utilizing engineered EVs as targeted delivery systems for anti-tumor drugs and overcoming drug resistance in the near future.
基金supported by Research Fund for the Doctoral Program of Higher Education of China(200802980004 and 20070298008)the National Natural Science Fundation of China (30872055)
文摘Glycoprotein from Ginkgo biloba kernel may be allergic. In this paper, the allergic proteins were identified with Western blotting, and the 32 kDa glycoprotein was purified with ion exchange chromatography and gel chromatography. With Western blotting, there were 3 allergic proteins with molecular weight 21, 32, and 36 kDa. With SDS-PAGE analysis and measurements of the protein and sugar contents, the isolation and purification technology of 32 kDa was confirmed. Ginkgo crude protein extract was precipitated with ammonium sulphate (saturation gradient: 40-80%). The precipitate was purified by chromatography with DEAE-cellulose 52, then chromatography with Sephadex G-200 and the target glycoprotein was finally obtained. The analysis results showed the molecule of the glycoprotein was 32.12 kDa and the ratio of protein to sugar was 20.56:1. In conclusion, the purification method could be used in preparation of the glycoprotein, and the study could provide a basis for the further research of the glycoprotein.
基金supported by the National Natural Science Foundation of China(21401097)
文摘A new coordination polymer [Cu2(OH)(nbta)(tib)(H2 O)2]n(1, H3 nbta = 5-nitro-1,2,3-benzenetricarboxylic acid, tib = 1-(4 H-1,2,4-triazol-4-yl)-4-(imidazol-1-yl)benzene) constructed from dimeric Cu(Ⅱ) units has been synthesized under hydrothermal conditions and structurally characterized by single-crystal X-ray diffraction analysis. The compound crystallizes in monoclinic system, space group I2/c, with a = 17.8474(9), b = 11.7588(6), c = 21.9221(11) ?, β = 104.419(5)°, V = 4455.7(4) ?3 and Z = 8. Compound 1 is a three-dimensional structure constructed from dimeric Cu(Ⅱ) units and presents a 2-nodal(3,5)-connected net. Moreover, the thermal stability and solid UV-Vis absorption spectra have been investigated.
基金supported by the Project of Science&Technology in Henan Province(No.172102310441)the National Natural Science Foundation of China(No.21571093)
文摘The cyanide groups of letrozole(1-[bis(4-cyanophenyl)methyl]-1,2,4-triazole) were hydrolyzed into the carboxylic groups under alkaline conditions. Then the hydrolysis product(1-[bis(4-carboxylphenyl)methyl]-1,2,4-triazole, H2ltzda) reacted with Co(NO3)2·6H2O in the presence of different bipyridl-typed ligands under hydrothermal conditions. As a result, two new Co(Ⅱ) MOFs were obtained, formulated as [Co2(ltzda)2(bpp)2]n(1) and {[Co2(ltzda)2(bpy)]·4H2O}n(2)(bpp = 1,3-di(4-pyridyl)propane, bpy = 4,4’-bipyridine). Two MOFs have been characterized by single-crystal X-ray diffraction, thermogravimetric analysis and magnetic measure. 1 displays a 2D metal-organic framework containing wave layers and 2 shows a pillared-layer structure existing in a 2-fold interpenetrated mode.
基金supported by the Natural Science Foundation of Henan Province(172102310333)
文摘A cobalt coordination polymer, {[Co_2(tdc)_2(bimm)_2]·(3 DMF)}n, was synthesized based on 2,5-thiophenedicarboxylic acid(H2 tdc) and bis(imidazol-1-yl)methane(bimm) mixed ligands. The asymmetric unit of the complex contains two Co^(^(2+)) cations, two tdc2-dianions, two neutral bimm ligands, and three free DMF molecules. In the complex, deprotonated tdc2-dianions alternately bridge the adjacent Co^(2+) cations to generate chains, which are further connected by the flexible bimm ligands to form a 3 D structure containing adamantanoid-like subunits. In topology, the structure of 1 represents a 4-connected uninodal two-fold interpenetrated dia(66) topology. Moreover, the solid UV-Vis absorption spectra of the complex have also been investigated.
基金supported by the National Natural Science Foundation of China(21401097)
文摘One two-dimensional coordination polymer with a formula of {[Cd(L)(imidazole)(H_2O)](H_2O)}n(1) was obtained by the synthetic reactions in aqueous solution using a newly synthesized H_2L(H_2L = methyl-3-hydroxy-5-carboxy-2-thiophenecarboxylate) ligand.Compound1 crystallizes in monoclinic system,space group C2/c with a = 18.3176(11),b = 8.5366(9),c =8.4152(5) ?,β = 101.789(6)o,V = 2797.1(3) ?~3,D_c = 1.979 g/cm^3,C_(10)H_(12)N_2O_7SCd,Mr = 416.68 F(000) = 1648,μ = 1.745 mm^(-1),F(000) = 1648,the final R = 0.0323 and wR = 0.0604 for 2604 observed reflections with I > 2s(I).Structure analyses reveal that the compound is constructed by dinuclear Cd(Ⅱ) clusters bridged by two hydroxyl oxygens of L^(2-)anions,which features a two-dimensional network with 4-connected sql topology.Furthermore,the compound exhibits high thermal stability and intense fluorescent emission,and could be explored for potentia luminescent materials.
基金financial support from Natural Science Foundation of Henan Province(No.222300420501)the Key Scientific and Technological Project of Henan province(No.212102210549)+1 种基金the Key Scientific Research Project of Higher Education of Henan Province(No.22A430007)National College Students Innovation and Entrepreneurship Training Program(No.202210482028)。
文摘An amphiphilic AIE photosensitizer has been successfully developed,which allows for easily inserting into the bacterial membranes.Binding experiments with phospholipid preliminary demonstrates its membrane specificity.As expected,it is proved to possess a broad-spectrum bacterial staining performance and photodynamic antibacterial activity toward S.aureus and E.coli.
基金supported by the National Natural Science Foundation of China(21401097)。
文摘Based on 5-dimethylamino-isophthalic acid(H2dia)and 1,2-bis(4-pyridyl)ethylene(bpe)mixed linkers,a new d10-configuration Zn(Ⅱ)coordination polymer material,[Zn(dia)(bpe)(H2O)]n(1),was synthesized.In the asymmetric unit,there are one Zn2+ion,two dia2-anions,one neutral bpe linker and one free H2O molecule.In the complex,adjacent Zn2+ions are alternately bridged by the dia2-dianions to generate chains which are further connected by flexible bpe ligands to form a two-dimensional(2 D)layer structure.In topology,the structure of 1 represents a non-interpenetrating 4-connected sql topology.Moreover,infrared spectroscopy,elemental analyses,thermogravimetric analysis,powder X-ray diffraction,solid UV-Vis absorption spectra and photoluminescent property of 1 are also investigated.
基金the funding support from the National Natural Science Foundation of China(No.52102348)the funding support from the National Natural Science Foundation of China(No.52173143)+6 种基金the funding support from the National Natural Science Foundation of China(No.22005259)the Science and Technology Innovation Talent Program of University in Henan Province(No.23HASTIT016)the funding support from China Postdoctoral Science Foundation(No.2021M701113)the Start-Up Grant(No.9610495)from City University of Hong Kongthe funding support from the National Natural Science Foundation of China(No.21905195)Natural Science Foundation of Tianjin City(No.20JCYBJC00800)the PEIYANG Young Scholars Program of Tianjin University(No.2020XRX-0023)。
文摘Although porphyrin-based metal-organic frameworks(MOFs)have been widely explored as photosensitizers for photodynamic therapy,how the size will affect the light-induced catalytic activity for generation of reactive oxygen species(ROS)still remain unclear.Herein,we first report the size-controlled synthesis of two-dimensional(2D)porphyrin-based PCN-134 MOF nanosheets by a two-step solvothermal method to explore the size effect on its PDT performance,thus yielding enhanced photodynamic antimicrobial therapy.By simply controlling the reaction temperature in the synthesis process,the bulk PCN-134 crystal,large PCN-134(L-PCN-134)nanosheets with a lateral size of 2–3μm and thickness of 33.2–37.5 nm and small PCN-134 nanosheets(S-PCN-134)with a lateral size of 160–180 nm and thickness of 9.1–9.7 nm were successfully prepared.Interestingly,the S-PCN-134 nanosheets exhibit much higher photodynamic activity for ROS generation than that of the bulk 3D PCN-134 crystal and L-PCN-134 nanosheets under a660 nm laser irradiation,suggesting that the photodynamic activity of PCN-134 MOF increases when the size reduces.Therefore,the S-PCN-134 nanosheets show much enhanced performance when used as a photosensitizer for photodynamic antimicrobial activity and wound healing.
基金The authors acknowledge financial support from Na- tional Natural Science Foundation of China （Nos. 21676113, 21402057, 21472059）, Youth Chen-Guang Project of Wuhan （No. 2016070204010098） the Minis- try-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, Shenzhen self-determined research funds of CCNU from the Colleges＇ Basic Research and Operation of MOE （No. CCNU16A02004）.
基金financial support from the Natural Science Foundation of Henan Province(No.222300420501)the Science and Technology Project of Henan Province(No.212102210549)+1 种基金the Key Scientific Research Project of Higher Education of Henan Province(No.22A430007)National College Students Innovation and Entrepreneurship Training Program(No.202110482010)。
文摘Three novel dithienylethenes modified by bifluoroboron β-diketonate fragments have been successfully developed. Upon blue light irradiation, they reached photostationary state within 2-5 s, as well as 100% conversion ratio and photocyclization quantum yield of > 0.70. Such fascinating photochromism were endowed by collaborative role of electron-withdrawing effect of BF_(2)bdk group to reduce HOMO-LUMO electronic gap for the open isomer, together with intramolecular hydrogen bonds and CH-π interactions favoring antiparallel conformation fixation. Moreover, they displayed specific discrimination and photoswitchable bacterial imaging for S. aureus.
