Municipal solid waste incineration tailings were used as lightweight aggregate(MSWIT-LA)in the preparation of specified density concrete to study the effects on compressive strength,axial compressive strength,flexural...Municipal solid waste incineration tailings were used as lightweight aggregate(MSWIT-LA)in the preparation of specified density concrete to study the effects on compressive strength,axial compressive strength,flexural strength,microhardness,total number of pores,pore area,and pore spacing.The results showed that the internal curing and morphological effects induced by an appropriate quantity of MSWIT-LA improved the compressive response of specified density concrete specimens,whereas an excessive quantity of MSWIT-LA significantly reduced their mechanical properties.An analysis of pore structure indicated that the addition of MSWIT-LA increased the total quantity of pores and promoted cement hydration,resulting in a denser microstructure than that of ordinary concrete.The results of a principal component analysis showed that the mechanical response of specified density concrete prepared with 25%MSWIT-LA was superior to that of an equivalent ordinary concrete.It was therefore concluded that MSWIT-LA can be feasibly applied to achieve excellent specified density concrete properties while utilising municipal solid waste incineration tailings to protect the environment and alleviate shortages of sand and gravel resources.展开更多
[Objective]The paper was to explore the comprehensive sexual attraction control technology of main pests in broccoli,and to improve the green control technology of main pests in broccoli.[Method]The comprehensive cont...[Objective]The paper was to explore the comprehensive sexual attraction control technology of main pests in broccoli,and to improve the green control technology of main pests in broccoli.[Method]The comprehensive control technology of sex attractants against major pests in broccoli was studied by using special sex attractants,lures and traps.[Result]The mixed quantities of Spodoptera litura,Spodoptera exigua and Plutella xylostella were fluctuated in a"W"shape in broccoli growing season in coastal area of Taizhou,central Zhejiang province,including seedling growth period(July to August),rosette and bud growth period(late September to early November),late harvest period of late-ripening broccoli(after early march).S.exigua and S.litura occurred severely at seedling stage;S.litura,S.exigua and P.xylostella occurred simultaneously at rosette and bud growth period;P.xylostella damaged florets of lateral branches in late harvest period.The trapping effects of combination A and E against S.litura,S.exigua and P.xylostella were the best;more than 1260 individuals of S.litura,S.exigua and P.xylostella were daily trapped per hectare,and the occurrence and damage of the three insect pests could be basically controlled.With the sex attract produced in Ningbo as an example,the combination of S.litura S type∶S.exigua S type∶P.xylostella blue type=1∶1∶4 distributed in five stars of clubs(space 10-20 cm)showed good comprehensive trapping effect in large area demonstration;traps were hung at the height of 0.5 m(0.2 m for P.xylostella),and lures were replaced once every 30-40 d.[Conclusion]Using special sex attractants,the comprehensive effects on S.litura,S.exigua and P.xylostella were determined at rosette and bud growth period of broccoli through combination modes,and supporting application technology were put forward,making great progress in exploring and improving sex attractant combination against insect pests.展开更多
Aiming at the complex corrosion degradation factors of reinforced concrete and clearing the deterioration mechanism in the constant stress state,a new type of constant current accelerated corrosion method in the salin...Aiming at the complex corrosion degradation factors of reinforced concrete and clearing the deterioration mechanism in the constant stress state,a new type of constant current accelerated corrosion method in the saline soil environment was developed.The three-dimensional paraffin isolation specimens and the three-dimensional penetration specimens were taken as the research objects,and the Cl−content and AC impedance Bode diagram were measured.The macro morphology and micro analysis were also used to evaluate the corrosion degradation laws of the two groups of specimens.A constant current three-factor system accelerated model was established for the current acceleration factor,chloride ion,and sulfate ion acceleration factor.The experimental results show that,in the constant stress test of the saline soil environmental conditions,the paraffin isolation layer can effectively isolate corrosive chloride ions,which is a brand-new research method of single factor variable control in the constant stress test.According to the basic corrosion data,the law of constant current acceleration test is summarized and divided into five corrosion degradation stages,and each stage has significant changes in the accelerated corrosion efficiency.The corrosion degradation of a constant stress test is the combined effect of constant current,positive and negative penetration of chloride ions and sulfate ions.展开更多
Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato ...Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.展开更多
Ballistic impact response of resistance-spot-welded(RSW)double-layered(2×1.6 mm)plates(190 mm×150 mm)for Q&P980 steel impacted by a round-nosed steel bullet(12 mm diameter and 30 mm length)was investigat...Ballistic impact response of resistance-spot-welded(RSW)double-layered(2×1.6 mm)plates(190 mm×150 mm)for Q&P980 steel impacted by a round-nosed steel bullet(12 mm diameter and 30 mm length)was investigated by using gas gun and high-speed camera system.The RSW specimens were spot welded using a 6 mm diameter electrode face producing a 7.2 mm diameter fusion zone of the spot weld.The ballistic curve and energy balance for the tests of the spot weld of the RSW specimens at different velocity were analyzed to characterize the ballistic behavior of the RSW specimens under bullet impact.The fracture mechanisms of the RSW specimens under bullet impact were presented.For the tests below the ballistic limit,the cracks initiated from the notch-tip and propagated along the faying surface or obliquely through the thickness depending on the impact velocity.For the tests above the ballistic limit,the plug fracture in the front plate of the RSW specimen could be caused by the thinning-induced necking in the BM near the HAZ,while the plug fracture in the rear plate of the RSW specimens may be consist of the circumferential cracking from the rear surface and the bending fracture of the hinged part of material.The effects of the electrode indentation and the weld interfaces on deformation and fracture of the RSW specimens under bullet impact were revealed.For the tests above the ballistic limit,the circumferential fracture from the rear surface of the RSW specimens was always initiated along the interior periphery of the electrode indentation and the crack paths were along the FZ/CGHAZ or CGHAZ/FGHAZ interface.When the circumferential crack also formed outside the electrode indentation,the fracture on the BM/HAZ interface could be found.On the front plate of the RSW specimens,the shear/bending induced cracking from the notch-tip were observed and the crack paths were along the FZ/CGHAZ or CGHAZ/FGHAZ interface.展开更多
Power product-service system(power PSS),which combines industrial electric products with electric energy services,is an effective solution for power enterprises under the background of the rapid development of power s...Power product-service system(power PSS),which combines industrial electric products with electric energy services,is an effective solution for power enterprises under the background of the rapid development of power systems.In the life cycle of power PSS,evaluation decision of power PSS alternatives is of great significance for subsequent implementation.To address the power PSS alternative evaluation problem,a power PSS evaluation framework is explored driven by the big data of stakeholder comments.Based on the multi-stakeholder comments of power PSS evaluation decision’s influence factors,the index system is constructed through analyzing and summarizing the co-occurrence matrix and semantic network diagram of high-frequency words.To determine the fuzzy index value of power PSS alternative,the stakeholders’vague opinions expressed by trapezoidal fuzzy number are integrated by group decision method.Fuzzy concept is introduced into the classical Technique for Order Preference by Similarity to an Ideal Solution(TOPSIS)method and fuzzy-TOPSIS method is put forward by using the fuzzy index value.The improved TOPSIS is adopted to sequence the power PSS alternatives.The case of power PSS evaluation of six alternatives for a power enterprise shows that the explored framework is effective and can provide a feasible solution for power PSS alternative evaluation.展开更多
[Objective] The paper was to explore the occurrence and damage regularity of Spodoptera litura in broccoli,and to reveal the annual movement regularity and seasonal fluctuation regularity of S.litura population.[Metho...[Objective] The paper was to explore the occurrence and damage regularity of Spodoptera litura in broccoli,and to reveal the annual movement regularity and seasonal fluctuation regularity of S.litura population.[Method] The monitoring data of sexual attractants at fixed points for 11 consecutive years were summarized and analyzed.[Result] The large occurrence frequency of S.litura in Taizhou was 36.4%;the annual movement showed a downward-opening parabolic form,and the movement trajectory was N =-18.416 t^(2)+182.51 t +179.71,t ={1,2,3,…,n}(n =11;r =0.691 7*).The seasonal fluctuation showed a trend of peak oscillation attenuation curve,and the total moth quantity gradually decreased from sowing to heading stage.The functional model of average daily quantity was m=0.000 2 d^(2)-0.067 5 d+6.797 3,d={1,2,3,…,n}(n =186,r=0.955 2**).Air temperature was the main external factor affecting the seasonal variation of quantity,and there was a significant correlation be-tween the number of ten-day moth trapping quantity and ten-day average temperature:M =0.243 T^(2)-6.935 1 T +46.681(n =108,r =0.560 2**)or M=2.734 4 T-35.446(n=108,r=0.500 3**).Therefore,the moth quantity in the field increased with the increase of ten-day average temperature,and the period from July to September with high temperature and less rainfall was the peak occurrence period of S.litura.[Conclusion] These models can be used to improve the prediction and early warning levels of S.litura in broccoli,and has good guiding significance for decision-making and prevention.展开更多
[Objective]The paper was to effectively control stem base rot of sweet potato.[Method]Chemical prevention and control test against stem base rot of sweet potato were conducted for consecutive three years from 2016 to ...[Objective]The paper was to effectively control stem base rot of sweet potato.[Method]Chemical prevention and control test against stem base rot of sweet potato were conducted for consecutive three years from 2016 to 2018.[Result]The use of carbendazim or carbendazim+mancozeb,and carbendazim+embamycin had significant control effect on stem base rot of sweet potato.However,the applied concentration should be higher than the recommended concentration.Especially the concentration of 50%carbendazim WP should be no more than 300 times,and the prevention and control effect of 100 times dilution would be better.[Conclusion]The types and concentrations of prevention and control agents are determined in the test,which will provide certain basis for chemical prevention and control against stem base rot of sweet potato.展开更多
The purpose of this study was to examine how parental involvement influences their adolescents’school truancy.Structural Equation Modeling(SEM)was utilized to explore the relationships among four dimensions of parent...The purpose of this study was to examine how parental involvement influences their adolescents’school truancy.Structural Equation Modeling(SEM)was utilized to explore the relationships among four dimensions of parental involvement,individual and family characteristics,and school truancy.The findings confirm the initial hypotheses that parental involvement has positive effects on adolescent truancy.The effects of parental involvement on school truancy,however,differed according to the dimensions of that involvement and adolescents’background characteristics.展开更多
Renewable technologies such as solar present some of the possibilities for indoor and outdoor lighting in the remotest rural Africa where grid connections may take ages.This paper examined the key determinants that dr...Renewable technologies such as solar present some of the possibilities for indoor and outdoor lighting in the remotest rural Africa where grid connections may take ages.This paper examined the key determinants that drive household lighting fuel using a nationally representative sample(14,415 households)across Rwanda.Results from a multinomial probit regression show that rural location,house ownership,household wealth,and nonfarm work are some of significant factors that influence lighting fuel choices in Rwanda.Robustness of the results indicates that household wealth levels and other regional differences are likely to influence choice probability for using clean energy sources such as solar confirming the need to priotise wealth generation.The study’s findings suggest the need for joint efforts by government and non-state actors to priotise household wealth generation,promotion of non-farm activities and improvement of infrastructures to reduce rural-urban bias and differences across the regions,assuming that wealth will motivate rural households to switch to clean energy sources.展开更多
This study aimed to explore groundwater potential zones in the EGMB of Alluri Seetharama Raju district, Andhra Pradesh, India, for drinking and agriculture purposes. To achieve this goal, 72 Vertical Electrical Soundi...This study aimed to explore groundwater potential zones in the EGMB of Alluri Seetharama Raju district, Andhra Pradesh, India, for drinking and agriculture purposes. To achieve this goal, 72 Vertical Electrical Soundings(VES) were conducted using the Schlumberger electrode configuration. The resistivity sounding data were analyzed to determine the aquifer thickness, basement depth, Dar-Zarrouk parameters,and aquifer transmissivity. Spatial distribution maps were generated for these parameters to understand the subsurface formation. The analysis revealed a linear groundwater potential zone(8.46 km~2) in the eastern part of the study area, extending in the NNE-SSW direction for 9.6 km. Six VES locations(P24, P27, P29,P30, P33, and P38) in this zone exhibit good potential(>30 m aquifer thickness), while the three VES locations(OP19, P5, and P46) in the central region are recommended for drilling bore wells. Additionally,moderate aquifer thickness(20–30 m) are identified in other VES locations(OP14, OP20, P4, P10, P12,P13, P15, P17, P18, P31, P46, and P50) along streams in the western and central part of the area, which can yield reasonable quantities of water. This information is useful for groundwater exploration and watershed management to meet the demands of tribal population in the study area.展开更多
Existing simulations of terrorist attacks do not consider individual variations.To overcome this lim-itation,we propose a framework to model heterogeneous behavior of individuals during terrorist attacks.We constructe...Existing simulations of terrorist attacks do not consider individual variations.To overcome this lim-itation,we propose a framework to model heterogeneous behavior of individuals during terrorist attacks.We constructed an emotional model that integrated personality and visual perception for pedestrians.The emotional model was then integrated with pedestrian relationship networks to establish a decision-making model that sup-ported pedestrians’altruistic behaviors.A mapping model has been developed to correlate antisocial personality traits with attack strategies employed by terrorists.Experiments demonstrate that the proposed algorithm can generate practical heterogeneous behaviors that align with existing psychological research findings.展开更多
Local markets in East Africa have been destroyed by raging fires,leading to the loss of life and property in the nearby communities.Electrical circuits,arson,and neglected charcoal stoves are the major causes of these...Local markets in East Africa have been destroyed by raging fires,leading to the loss of life and property in the nearby communities.Electrical circuits,arson,and neglected charcoal stoves are the major causes of these fires.Previous methods,i.e.,satellites,are expensive to maintain and cause unnecessary delays.Also,unit-smoke detectors are highly prone to false alerts.In this paper,an Interval Type-2 TSK fuzzy model for an intelligent lightweight fire intensity detection algorithm with decision-making in low-power devices is proposed using a sparse inference rules approach.A free open–source MATLAB/Simulink fuzzy toolbox integrated into MATLAB 2018a is used to investigate the performance of the Interval Type-2 fuzzy model.Two crisp input parameters,namely:FIT and FIG��are used.Results show that the Interval Type-2 model achieved an accuracy value of FIO�=98.2%,MAE=1.3010,MSE=1.6938 and RMSE=1.3015 using regression analysis.The study shall assist the firefighting personnel in fully understanding and mitigating the current level of fire danger.As a result,the proposed solution can be fully implemented in low-cost,low-power fire detection systems to monitor the state of fire with improved accuracy and reduced false alerts.Through informed decision-making in low-cost fire detection devices,early warning notifications can be provided to aid in the rapid evacuation of people,thereby improving fire safety surveillance,management,and protection for the market community.展开更多
Increasing risks of incidental and occupational exposures to two-dimensional transition metal dichalcogenides(2D TMDCs)due to their broad application in various areas raised their public health concerns.While the comp...Increasing risks of incidental and occupational exposures to two-dimensional transition metal dichalcogenides(2D TMDCs)due to their broad application in various areas raised their public health concerns.While the composition-dependent cytotoxicity of 2D TMDCs has been well-recognized,how the outer chalcogenide atoms and inner transition metal atoms differentially contribute to their perturbation on cell homeostasis at non-lethal doses remains to be identified.In the present work,we compared the autophagy induction and related mechanisms in response to WS_(2),NbS_(2),WSe_(2)and Nb Se_(2)nanosheets exposures in MH-S murine alveolar macrophages.All these 2D TMDCs had comparable physicochemical properties,overall cytotoxicity and capability in triggering autophagy in MH-S cells,but showed outer chalcogen-dependent subcellular localization and activation of autophagy pathways.Specifically,WS_(2)and NbS_(2)nanosheets adhered on the cell surface and internalized in the lysosomes,and triggered m TOR-dependent activation of autophagy.Meanwhile,WSe_(2)and Nb Se_(2)nanosheets had extensive distribution in cytoplasm of MH-S cells and induced autophagy in an m TOR-independent manner.Furthermore,the 2D TMDCs-induced perturbation on autophagy aggravated the cytotoxicity of respirable benzo[a]pyrene.These findings provide a deeper insight into the potential health risk of environmental 2D TMDCs from the perspective of homeostasis perturbation.展开更多
By means of the multilinear variable separation(MLVS) approach, new interaction solutions with low-dimensional arbitrary functions of the(2+1)-dimensional Nizhnik–Novikov–Veselovtype system are constructed. Four-dro...By means of the multilinear variable separation(MLVS) approach, new interaction solutions with low-dimensional arbitrary functions of the(2+1)-dimensional Nizhnik–Novikov–Veselovtype system are constructed. Four-dromion structure, ring-parabolic soliton structure and corresponding fusion phenomena for the physical quantity U =λ(lnf)_(xy) are revealed for the first time. This MLVS approach can also be used to deal with the(2+1)-dimensional Sasa–Satsuma system.展开更多
In the current research,a thorough examination unfolds concerning the attributes of magnetohydrodynamic(MHD)boundary layer flow and heat transfer inherent to nanoliquids derived from Sisko Al_(2)O_(3)-Eg and TiO_(2)-E...In the current research,a thorough examination unfolds concerning the attributes of magnetohydrodynamic(MHD)boundary layer flow and heat transfer inherent to nanoliquids derived from Sisko Al_(2)O_(3)-Eg and TiO_(2)-Eg compositions.Such nanoliquids are subjected to an extending surface.Consideration is duly given to slip boundary conditions,as well as the effects stemming from variable viscosity and variable thermal conductivity.The analytical approach applied involves the application of suitable similarity transformations.These conversions serve to transform the initial set of complex nonlinear partial differential equations into a more manageable assembly of ordinary differential equations.Through the utilization of the FEM,these reformulated equations are solved,considering the specified boundary conditions.The outcomes attained are graphically depicted by means of plots and tables.These visual aids facilitate a comprehensive exploration of how diverse parameters exert influence over the distributions of velocity,temperature,and concentration.Furthermore,detailed scrutiny is directed towards the fluctuations characterizing pivotal parameters,viz.,Nusselt number,skin-friction coefficient,and Sherwood number.It is identified that the Nusselt number showcases a diminishing trend coinciding with increasing values of the volume fraction parameter(φ).This trend remains consistent regardless of whether the nanoliquid under consideration is Al_(2)O_(3)-Eg or TiO_(2)-Eg based.In contrast,both the skin-friction coefficient and Sherwood number assume lower values as the volume fraction parameter(φ)escalates.This pattern remains congruent across both classifications of nanoliquids.The findings of the study impart valuable insights into the complex interplay governing the characteristics of HMT pertaining to Sisko Al_(2)O_(3)-Eg and TiO_(2)-Eg nanoliquids along an extending surface.展开更多
[Objective]The paper was to study the relationship between rice leaf roller,rice planthopper and spider and their ecological regulation ability in the field.[Method]The population dynamics of rice leaf roller,rice pla...[Objective]The paper was to study the relationship between rice leaf roller,rice planthopper and spider and their ecological regulation ability in the field.[Method]The population dynamics of rice leaf roller,rice planthopper and spider at three different sowing and transplanting periods of single cropping hybrid rice in the treatment area with combination of 3 drug formulations and untreated area were monitored.[Result]There were significant or extremely significant positive correlations among rice leaf roller,rice planthopper and spider in single cropping hybrid rice.However,with the delay of planting and transplanting period,the population fluctuation crest of rice leaf roller and rice planthopper decreased,but the intensity increased with the advance of time,and the natural ecological regulation ability was relatively improved.[Conclusion]The sowing period of single cropping hybrid rice is delayed 5-10 d compared with conventional rice,that is,sowing in late May and early June.The seedling age of 20-30 d is beneficial not only to reduce the initial population quantity of rice leaf roller and rice planthopper in the field,but also to increase the population quantity of spiders.展开更多
Carbapenem is one of the few available drugs to treat multidrug-resistance Gram-negative bacteria infections.Recently,the plasmid-mediated spread of the carbapenem resistance gene bla_(NDM) poses a significant threat ...Carbapenem is one of the few available drugs to treat multidrug-resistance Gram-negative bacteria infections.Recently,the plasmid-mediated spread of the carbapenem resistance gene bla_(NDM) poses a significant threat to public health,requiring global monitoring and surveillance.Here,we used both short-read(n=2461)and long-read(n=546)sequencing data to characterize the global distribution of bla_(NDM).We analyzed the replicon type of bla_(NDM)-positive plasmids and found that the dominant plasmid type was different in diverse geographical locations.Although bla_(NDM)gene has been transferred across diverse countries,its genetic backgrounds are highly conserved,and the mobile genetic element ISAba125,IS5,and IS26 may play an important role in the mobilization of bla_(NDM).A significant association was observed between host origin and gene presence/deletion variation on IncX3 plasmid,which may be a key factor in the bacterial adaption to diverse hosts.In this study,we analyzed the diversity,distribution and transmission of bla_(NDM)-positive plasmids from a global perspective,and emphasize the importance of plasmid analysis for understanding the evolution and adaptation of bla_(NDM)-positive plasmids and their co-evolution with bacterial genomes(resistome).展开更多
Industrial manufacturing of bioproducts,especially bioethanol,can benefit from high-temperature fermentation,which requires the use of thermotolerant yeast strains.Mitochondrial activity in yeast is closely related to...Industrial manufacturing of bioproducts,especially bioethanol,can benefit from high-temperature fermentation,which requires the use of thermotolerant yeast strains.Mitochondrial activity in yeast is closely related to its over-all metabolism.However,the mitochondrial respiratory changes in response to adaptive thermotolerance are still poorly understood and have been rarely utilized for developing thermotolerant yeast cell factories.Here,adap-tive evolution and transcriptional sequencing,as well as whole-genome-level gene knockout,were used to obtain a thermotolerant strain of Saccharomyces cerevisiae.Furthermore,thermotolerance and bioethanol production efficiency of the engineered strain were examined.Physiological evaluation showed the boosted fermentation ca-pacity and suppressed mitochondrial respiratory activity in the thermotolerant strain.The improved fermentation produced an increased supply of adenosine triphosphate required for more active energy-consuming pathways.Transcriptome analysis revealed significant changes in the expression of the genes involved in the mitochondrial respiratory chain.Evaluation of mitochondria-associated gene knockout confirmed that ADK1,DOC1,or MET7 were the key factors for the adaptive evolution of thermotolerance in the engineered yeast strain.Intriguingly,overexpression of DOC1 with TEF1 promoter regulation led to a 10.1%increase in ethanol production at 42℃.The relationships between thermotolerance,mitochondrial activity,and respiration were explored,and a ther-motolerant yeast strain was developed by altering the expression of mitochondrial respiration-related genes.This study provides a better understanding on the physiological mechanism of adaptive evolution of thermotolerance in yeast.展开更多
LaFe03 perovskite with a porous fibrous structure was successfully synthesized using a sunflower seed shell as a template.To investigate the effects of this template,a sample was prepared without a template via the sa...LaFe03 perovskite with a porous fibrous structure was successfully synthesized using a sunflower seed shell as a template.To investigate the effects of this template,a sample was prepared without a template via the same procedure.Through various characterization techniques,such as X-ray diffraction,scanning electron microscopy,Fourier transform infrared spectroscopy,N2 adsorption-desorption analysis,X-ray photoelectron spectroscopy,oxygen temperature programed desorption,and hydrogen temperature programed reduction,the physiochemical properties of the samples were investigated.The results showed that the sample made with a template had a larger surface area and a larger amount of adsorbed oxygen,which further illustrated that the sunflower seed shell template had a significant impact on the physiochemical properties of the samples.Furthermore,we explored the catalytic activity for nitric oxide(NO)oxidation,and studied the factors affecting it,which highlighted its potential application in automobile exhausts.展开更多
基金Funded by the National Natural Science Foundation of China(Nos.U21A20150,52208249,51878153,52108219,52008196,52178216)Research and Demonstration of Key Technologies of Green and Smart Highways in Gansu Province(No.21ZD3GA002)+1 种基金Gansu Provincial Natural Science Foundation(No.23JRRA799)Key Projects of Chongqing Science and Technology Bureau(No.2021jscx-jbgs0029)。
文摘Municipal solid waste incineration tailings were used as lightweight aggregate(MSWIT-LA)in the preparation of specified density concrete to study the effects on compressive strength,axial compressive strength,flexural strength,microhardness,total number of pores,pore area,and pore spacing.The results showed that the internal curing and morphological effects induced by an appropriate quantity of MSWIT-LA improved the compressive response of specified density concrete specimens,whereas an excessive quantity of MSWIT-LA significantly reduced their mechanical properties.An analysis of pore structure indicated that the addition of MSWIT-LA increased the total quantity of pores and promoted cement hydration,resulting in a denser microstructure than that of ordinary concrete.The results of a principal component analysis showed that the mechanical response of specified density concrete prepared with 25%MSWIT-LA was superior to that of an equivalent ordinary concrete.It was therefore concluded that MSWIT-LA can be feasibly applied to achieve excellent specified density concrete properties while utilising municipal solid waste incineration tailings to protect the environment and alleviate shortages of sand and gravel resources.
基金Supported by Science and Technology Project of Taizhou City"Research,Integration and Industrial-ization Development of Broccoli Production Technology Coping with Japan’s Affirmative List System(Research and Application of Key Technologies in Promoting Broccoli Industry)"(071TG04)Taizhou Broccoli Breeding and Promotion Integration Innovation Team Project(TRCL[2014]No.7)。
文摘[Objective]The paper was to explore the comprehensive sexual attraction control technology of main pests in broccoli,and to improve the green control technology of main pests in broccoli.[Method]The comprehensive control technology of sex attractants against major pests in broccoli was studied by using special sex attractants,lures and traps.[Result]The mixed quantities of Spodoptera litura,Spodoptera exigua and Plutella xylostella were fluctuated in a"W"shape in broccoli growing season in coastal area of Taizhou,central Zhejiang province,including seedling growth period(July to August),rosette and bud growth period(late September to early November),late harvest period of late-ripening broccoli(after early march).S.exigua and S.litura occurred severely at seedling stage;S.litura,S.exigua and P.xylostella occurred simultaneously at rosette and bud growth period;P.xylostella damaged florets of lateral branches in late harvest period.The trapping effects of combination A and E against S.litura,S.exigua and P.xylostella were the best;more than 1260 individuals of S.litura,S.exigua and P.xylostella were daily trapped per hectare,and the occurrence and damage of the three insect pests could be basically controlled.With the sex attract produced in Ningbo as an example,the combination of S.litura S type∶S.exigua S type∶P.xylostella blue type=1∶1∶4 distributed in five stars of clubs(space 10-20 cm)showed good comprehensive trapping effect in large area demonstration;traps were hung at the height of 0.5 m(0.2 m for P.xylostella),and lures were replaced once every 30-40 d.[Conclusion]Using special sex attractants,the comprehensive effects on S.litura,S.exigua and P.xylostella were determined at rosette and bud growth period of broccoli through combination modes,and supporting application technology were put forward,making great progress in exploring and improving sex attractant combination against insect pests.
基金Funded by the National Natural Science Foundation of China (U21A20150,52208249,51878153,52008196,and 52178216)the National Natural Science Foundation of China Youth Science (52108219)+3 种基金the Deterioration Characteristics and Mechanisms of Cement Concrete with CWPM under Coupled Thermo-Hydro-Chemical Effect,the Youth Science and Technology Foundation of Gansu Province (22JR5RA288)the Research and Demonstration of Key Technologies of Green and Smart Highways in Gansu Province (21ZD3GA002)the Natural Science Innovation Foundation of Gansu Higher Education Institutions (2022CYZC-25)the Support from Research on Key Technologies of Durability Repair of Highway Concrete Bridges,Key Projects of Chongqing Science and Technology Bureau (cstc2021jscx-jbgs0029)。
文摘Aiming at the complex corrosion degradation factors of reinforced concrete and clearing the deterioration mechanism in the constant stress state,a new type of constant current accelerated corrosion method in the saline soil environment was developed.The three-dimensional paraffin isolation specimens and the three-dimensional penetration specimens were taken as the research objects,and the Cl−content and AC impedance Bode diagram were measured.The macro morphology and micro analysis were also used to evaluate the corrosion degradation laws of the two groups of specimens.A constant current three-factor system accelerated model was established for the current acceleration factor,chloride ion,and sulfate ion acceleration factor.The experimental results show that,in the constant stress test of the saline soil environmental conditions,the paraffin isolation layer can effectively isolate corrosive chloride ions,which is a brand-new research method of single factor variable control in the constant stress test.According to the basic corrosion data,the law of constant current acceleration test is summarized and divided into five corrosion degradation stages,and each stage has significant changes in the accelerated corrosion efficiency.The corrosion degradation of a constant stress test is the combined effect of constant current,positive and negative penetration of chloride ions and sulfate ions.
基金funded by the Scientific Research Fund of College of Science&Technology,Ningbo University for the Introduction of High-level Talents,China(RC190006)。
文摘Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.
基金supported by the National Natural Science Foundation of China,China under the grant No.11372149K.C.Wong Magna Fund in Ningbo University。
文摘Ballistic impact response of resistance-spot-welded(RSW)double-layered(2×1.6 mm)plates(190 mm×150 mm)for Q&P980 steel impacted by a round-nosed steel bullet(12 mm diameter and 30 mm length)was investigated by using gas gun and high-speed camera system.The RSW specimens were spot welded using a 6 mm diameter electrode face producing a 7.2 mm diameter fusion zone of the spot weld.The ballistic curve and energy balance for the tests of the spot weld of the RSW specimens at different velocity were analyzed to characterize the ballistic behavior of the RSW specimens under bullet impact.The fracture mechanisms of the RSW specimens under bullet impact were presented.For the tests below the ballistic limit,the cracks initiated from the notch-tip and propagated along the faying surface or obliquely through the thickness depending on the impact velocity.For the tests above the ballistic limit,the plug fracture in the front plate of the RSW specimen could be caused by the thinning-induced necking in the BM near the HAZ,while the plug fracture in the rear plate of the RSW specimens may be consist of the circumferential cracking from the rear surface and the bending fracture of the hinged part of material.The effects of the electrode indentation and the weld interfaces on deformation and fracture of the RSW specimens under bullet impact were revealed.For the tests above the ballistic limit,the circumferential fracture from the rear surface of the RSW specimens was always initiated along the interior periphery of the electrode indentation and the crack paths were along the FZ/CGHAZ or CGHAZ/FGHAZ interface.When the circumferential crack also formed outside the electrode indentation,the fracture on the BM/HAZ interface could be found.On the front plate of the RSW specimens,the shear/bending induced cracking from the notch-tip were observed and the crack paths were along the FZ/CGHAZ or CGHAZ/FGHAZ interface.
基金This study is supported by the Teaching and Research Fund of Ningbo University(Grant No.JYXMXYB202000).
文摘Power product-service system(power PSS),which combines industrial electric products with electric energy services,is an effective solution for power enterprises under the background of the rapid development of power systems.In the life cycle of power PSS,evaluation decision of power PSS alternatives is of great significance for subsequent implementation.To address the power PSS alternative evaluation problem,a power PSS evaluation framework is explored driven by the big data of stakeholder comments.Based on the multi-stakeholder comments of power PSS evaluation decision’s influence factors,the index system is constructed through analyzing and summarizing the co-occurrence matrix and semantic network diagram of high-frequency words.To determine the fuzzy index value of power PSS alternative,the stakeholders’vague opinions expressed by trapezoidal fuzzy number are integrated by group decision method.Fuzzy concept is introduced into the classical Technique for Order Preference by Similarity to an Ideal Solution(TOPSIS)method and fuzzy-TOPSIS method is put forward by using the fuzzy index value.The improved TOPSIS is adopted to sequence the power PSS alternatives.The case of power PSS evaluation of six alternatives for a power enterprise shows that the explored framework is effective and can provide a feasible solution for power PSS alternative evaluation.
基金Supported by Science and Technology Planning Project of Taizhou City (071TG04)Taizhou Broccoli Breeding,Propagation and Promotion Integrated Innovation Team Project (TRCL[2014]7)。
文摘[Objective] The paper was to explore the occurrence and damage regularity of Spodoptera litura in broccoli,and to reveal the annual movement regularity and seasonal fluctuation regularity of S.litura population.[Method] The monitoring data of sexual attractants at fixed points for 11 consecutive years were summarized and analyzed.[Result] The large occurrence frequency of S.litura in Taizhou was 36.4%;the annual movement showed a downward-opening parabolic form,and the movement trajectory was N =-18.416 t^(2)+182.51 t +179.71,t ={1,2,3,…,n}(n =11;r =0.691 7*).The seasonal fluctuation showed a trend of peak oscillation attenuation curve,and the total moth quantity gradually decreased from sowing to heading stage.The functional model of average daily quantity was m=0.000 2 d^(2)-0.067 5 d+6.797 3,d={1,2,3,…,n}(n =186,r=0.955 2**).Air temperature was the main external factor affecting the seasonal variation of quantity,and there was a significant correlation be-tween the number of ten-day moth trapping quantity and ten-day average temperature:M =0.243 T^(2)-6.935 1 T +46.681(n =108,r =0.560 2**)or M=2.734 4 T-35.446(n=108,r=0.500 3**).Therefore,the moth quantity in the field increased with the increase of ten-day average temperature,and the period from July to September with high temperature and less rainfall was the peak occurrence period of S.litura.[Conclusion] These models can be used to improve the prediction and early warning levels of S.litura in broccoli,and has good guiding significance for decision-making and prevention.
基金Major Science and Technology Project for New Variety Breeding of Agriculture(Grain)in Zhejiang Province"Sweet Potato,Yam Germplasm Screening and New Variety Breeding"(2016C 02050-7-3)Key Agricultural Science and Technology Project of Taizhou City"Study on Pathogen Identification,Occurrence Regularity and Prevention and Control Technology of Sweet Potato Stem Base Rot(1701ny12)+2 种基金China Agricultural(Sweet Potato)Industry Research System“Fresh Sweet Potato Improvement"(CARS-10-C-17)Project of Guangzhou Comprehensive Test Station(CARS-10-B-4)2017 Special Project on Agricultural Development and Rural Work in Guangdong Province"Creation and Application of New Sweet Potato Varieties for High Quality and Resistance".
文摘[Objective]The paper was to effectively control stem base rot of sweet potato.[Method]Chemical prevention and control test against stem base rot of sweet potato were conducted for consecutive three years from 2016 to 2018.[Result]The use of carbendazim or carbendazim+mancozeb,and carbendazim+embamycin had significant control effect on stem base rot of sweet potato.However,the applied concentration should be higher than the recommended concentration.Especially the concentration of 50%carbendazim WP should be no more than 300 times,and the prevention and control effect of 100 times dilution would be better.[Conclusion]The types and concentrations of prevention and control agents are determined in the test,which will provide certain basis for chemical prevention and control against stem base rot of sweet potato.
文摘The purpose of this study was to examine how parental involvement influences their adolescents’school truancy.Structural Equation Modeling(SEM)was utilized to explore the relationships among four dimensions of parental involvement,individual and family characteristics,and school truancy.The findings confirm the initial hypotheses that parental involvement has positive effects on adolescent truancy.The effects of parental involvement on school truancy,however,differed according to the dimensions of that involvement and adolescents’background characteristics.
文摘Renewable technologies such as solar present some of the possibilities for indoor and outdoor lighting in the remotest rural Africa where grid connections may take ages.This paper examined the key determinants that drive household lighting fuel using a nationally representative sample(14,415 households)across Rwanda.Results from a multinomial probit regression show that rural location,house ownership,household wealth,and nonfarm work are some of significant factors that influence lighting fuel choices in Rwanda.Robustness of the results indicates that household wealth levels and other regional differences are likely to influence choice probability for using clean energy sources such as solar confirming the need to priotise wealth generation.The study’s findings suggest the need for joint efforts by government and non-state actors to priotise household wealth generation,promotion of non-farm activities and improvement of infrastructures to reduce rural-urban bias and differences across the regions,assuming that wealth will motivate rural households to switch to clean energy sources.
文摘This study aimed to explore groundwater potential zones in the EGMB of Alluri Seetharama Raju district, Andhra Pradesh, India, for drinking and agriculture purposes. To achieve this goal, 72 Vertical Electrical Soundings(VES) were conducted using the Schlumberger electrode configuration. The resistivity sounding data were analyzed to determine the aquifer thickness, basement depth, Dar-Zarrouk parameters,and aquifer transmissivity. Spatial distribution maps were generated for these parameters to understand the subsurface formation. The analysis revealed a linear groundwater potential zone(8.46 km~2) in the eastern part of the study area, extending in the NNE-SSW direction for 9.6 km. Six VES locations(P24, P27, P29,P30, P33, and P38) in this zone exhibit good potential(>30 m aquifer thickness), while the three VES locations(OP19, P5, and P46) in the central region are recommended for drilling bore wells. Additionally,moderate aquifer thickness(20–30 m) are identified in other VES locations(OP14, OP20, P4, P10, P12,P13, P15, P17, P18, P31, P46, and P50) along streams in the western and central part of the area, which can yield reasonable quantities of water. This information is useful for groundwater exploration and watershed management to meet the demands of tribal population in the study area.
基金Supported by the Natural Science Foundation of Zhejiang Province(LZ23F020005)Ningbo Science Technology Plan projects(2022Z077 and 2021S091).
文摘Existing simulations of terrorist attacks do not consider individual variations.To overcome this lim-itation,we propose a framework to model heterogeneous behavior of individuals during terrorist attacks.We constructed an emotional model that integrated personality and visual perception for pedestrians.The emotional model was then integrated with pedestrian relationship networks to establish a decision-making model that sup-ported pedestrians’altruistic behaviors.A mapping model has been developed to correlate antisocial personality traits with attack strategies employed by terrorists.Experiments demonstrate that the proposed algorithm can generate practical heterogeneous behaviors that align with existing psychological research findings.
文摘Local markets in East Africa have been destroyed by raging fires,leading to the loss of life and property in the nearby communities.Electrical circuits,arson,and neglected charcoal stoves are the major causes of these fires.Previous methods,i.e.,satellites,are expensive to maintain and cause unnecessary delays.Also,unit-smoke detectors are highly prone to false alerts.In this paper,an Interval Type-2 TSK fuzzy model for an intelligent lightweight fire intensity detection algorithm with decision-making in low-power devices is proposed using a sparse inference rules approach.A free open–source MATLAB/Simulink fuzzy toolbox integrated into MATLAB 2018a is used to investigate the performance of the Interval Type-2 fuzzy model.Two crisp input parameters,namely:FIT and FIG��are used.Results show that the Interval Type-2 model achieved an accuracy value of FIO�=98.2%,MAE=1.3010,MSE=1.6938 and RMSE=1.3015 using regression analysis.The study shall assist the firefighting personnel in fully understanding and mitigating the current level of fire danger.As a result,the proposed solution can be fully implemented in low-cost,low-power fire detection systems to monitor the state of fire with improved accuracy and reduced false alerts.Through informed decision-making in low-cost fire detection devices,early warning notifications can be provided to aid in the rapid evacuation of people,thereby improving fire safety surveillance,management,and protection for the market community.
基金supported by the Special Scientific Research Fund for Talents Introduced of Hebei Agricultural University (No.YJ2019030)the National Natural Science Foundation of China (Nos.22276042,21906035)the Pearl River Young Talents Program of Guangdong Province (No.2017GC010269)。
文摘Increasing risks of incidental and occupational exposures to two-dimensional transition metal dichalcogenides(2D TMDCs)due to their broad application in various areas raised their public health concerns.While the composition-dependent cytotoxicity of 2D TMDCs has been well-recognized,how the outer chalcogenide atoms and inner transition metal atoms differentially contribute to their perturbation on cell homeostasis at non-lethal doses remains to be identified.In the present work,we compared the autophagy induction and related mechanisms in response to WS_(2),NbS_(2),WSe_(2)and Nb Se_(2)nanosheets exposures in MH-S murine alveolar macrophages.All these 2D TMDCs had comparable physicochemical properties,overall cytotoxicity and capability in triggering autophagy in MH-S cells,but showed outer chalcogen-dependent subcellular localization and activation of autophagy pathways.Specifically,WS_(2)and NbS_(2)nanosheets adhered on the cell surface and internalized in the lysosomes,and triggered m TOR-dependent activation of autophagy.Meanwhile,WSe_(2)and Nb Se_(2)nanosheets had extensive distribution in cytoplasm of MH-S cells and induced autophagy in an m TOR-independent manner.Furthermore,the 2D TMDCs-induced perturbation on autophagy aggravated the cytotoxicity of respirable benzo[a]pyrene.These findings provide a deeper insight into the potential health risk of environmental 2D TMDCs from the perspective of homeostasis perturbation.
基金supported by the National Natural Science Foundation of China (11771395)。
文摘By means of the multilinear variable separation(MLVS) approach, new interaction solutions with low-dimensional arbitrary functions of the(2+1)-dimensional Nizhnik–Novikov–Veselovtype system are constructed. Four-dromion structure, ring-parabolic soliton structure and corresponding fusion phenomena for the physical quantity U =λ(lnf)_(xy) are revealed for the first time. This MLVS approach can also be used to deal with the(2+1)-dimensional Sasa–Satsuma system.
文摘In the current research,a thorough examination unfolds concerning the attributes of magnetohydrodynamic(MHD)boundary layer flow and heat transfer inherent to nanoliquids derived from Sisko Al_(2)O_(3)-Eg and TiO_(2)-Eg compositions.Such nanoliquids are subjected to an extending surface.Consideration is duly given to slip boundary conditions,as well as the effects stemming from variable viscosity and variable thermal conductivity.The analytical approach applied involves the application of suitable similarity transformations.These conversions serve to transform the initial set of complex nonlinear partial differential equations into a more manageable assembly of ordinary differential equations.Through the utilization of the FEM,these reformulated equations are solved,considering the specified boundary conditions.The outcomes attained are graphically depicted by means of plots and tables.These visual aids facilitate a comprehensive exploration of how diverse parameters exert influence over the distributions of velocity,temperature,and concentration.Furthermore,detailed scrutiny is directed towards the fluctuations characterizing pivotal parameters,viz.,Nusselt number,skin-friction coefficient,and Sherwood number.It is identified that the Nusselt number showcases a diminishing trend coinciding with increasing values of the volume fraction parameter(φ).This trend remains consistent regardless of whether the nanoliquid under consideration is Al_(2)O_(3)-Eg or TiO_(2)-Eg based.In contrast,both the skin-friction coefficient and Sherwood number assume lower values as the volume fraction parameter(φ)escalates.This pattern remains congruent across both classifications of nanoliquids.The findings of the study impart valuable insights into the complex interplay governing the characteristics of HMT pertaining to Sisko Al_(2)O_(3)-Eg and TiO_(2)-Eg nanoliquids along an extending surface.
基金Supported by Science and Technology Project of Taizhou City(14NY14)Key Research and De-velopment Program of Zhejiang Province(2016C02050-3-4)
文摘[Objective]The paper was to study the relationship between rice leaf roller,rice planthopper and spider and their ecological regulation ability in the field.[Method]The population dynamics of rice leaf roller,rice planthopper and spider at three different sowing and transplanting periods of single cropping hybrid rice in the treatment area with combination of 3 drug formulations and untreated area were monitored.[Result]There were significant or extremely significant positive correlations among rice leaf roller,rice planthopper and spider in single cropping hybrid rice.However,with the delay of planting and transplanting period,the population fluctuation crest of rice leaf roller and rice planthopper decreased,but the intensity increased with the advance of time,and the natural ecological regulation ability was relatively improved.[Conclusion]The sowing period of single cropping hybrid rice is delayed 5-10 d compared with conventional rice,that is,sowing in late May and early June.The seedling age of 20-30 d is beneficial not only to reduce the initial population quantity of rice leaf roller and rice planthopper in the field,but also to increase the population quantity of spiders.
基金supported by the Laboratory of Lingnan Modern Agriculture Project(NT2021006)National Natural Science Foundation of China(32273054).
文摘Carbapenem is one of the few available drugs to treat multidrug-resistance Gram-negative bacteria infections.Recently,the plasmid-mediated spread of the carbapenem resistance gene bla_(NDM) poses a significant threat to public health,requiring global monitoring and surveillance.Here,we used both short-read(n=2461)and long-read(n=546)sequencing data to characterize the global distribution of bla_(NDM).We analyzed the replicon type of bla_(NDM)-positive plasmids and found that the dominant plasmid type was different in diverse geographical locations.Although bla_(NDM)gene has been transferred across diverse countries,its genetic backgrounds are highly conserved,and the mobile genetic element ISAba125,IS5,and IS26 may play an important role in the mobilization of bla_(NDM).A significant association was observed between host origin and gene presence/deletion variation on IncX3 plasmid,which may be a key factor in the bacterial adaption to diverse hosts.In this study,we analyzed the diversity,distribution and transmission of bla_(NDM)-positive plasmids from a global perspective,and emphasize the importance of plasmid analysis for understanding the evolution and adaptation of bla_(NDM)-positive plasmids and their co-evolution with bacterial genomes(resistome).
基金supported by the National Key Research and De-velopment Program of China (2021YFC2103300),Research Equipment Program of Chinese Academy of Sciences (YJKYYQ20170023)National Natural Science Foundation of China (32071423,31470214,and 32200067)+1 种基金Natural Science Foundation of Hebei Province (C2020204013)Development Program Projects of Hebei Province (22322905D).
文摘Industrial manufacturing of bioproducts,especially bioethanol,can benefit from high-temperature fermentation,which requires the use of thermotolerant yeast strains.Mitochondrial activity in yeast is closely related to its over-all metabolism.However,the mitochondrial respiratory changes in response to adaptive thermotolerance are still poorly understood and have been rarely utilized for developing thermotolerant yeast cell factories.Here,adap-tive evolution and transcriptional sequencing,as well as whole-genome-level gene knockout,were used to obtain a thermotolerant strain of Saccharomyces cerevisiae.Furthermore,thermotolerance and bioethanol production efficiency of the engineered strain were examined.Physiological evaluation showed the boosted fermentation ca-pacity and suppressed mitochondrial respiratory activity in the thermotolerant strain.The improved fermentation produced an increased supply of adenosine triphosphate required for more active energy-consuming pathways.Transcriptome analysis revealed significant changes in the expression of the genes involved in the mitochondrial respiratory chain.Evaluation of mitochondria-associated gene knockout confirmed that ADK1,DOC1,or MET7 were the key factors for the adaptive evolution of thermotolerance in the engineered yeast strain.Intriguingly,overexpression of DOC1 with TEF1 promoter regulation led to a 10.1%increase in ethanol production at 42℃.The relationships between thermotolerance,mitochondrial activity,and respiration were explored,and a ther-motolerant yeast strain was developed by altering the expression of mitochondrial respiration-related genes.This study provides a better understanding on the physiological mechanism of adaptive evolution of thermotolerance in yeast.
基金the National Natural Science Foundation of China(Grant No.21607086)Zhejiang Province Public Welfare Technology Application Research Project(No.2016C33024)+3 种基金Scientific and Technological program of Ningbo City(Nos.2016C51025,2017C50031)Natural Science Foundation of Ningbo(No.2017A610061)Natural Science Foundation of Zhejiang Province(No.LY18B010003)K.C.Wong Magna Fund in Ningbo University.
文摘LaFe03 perovskite with a porous fibrous structure was successfully synthesized using a sunflower seed shell as a template.To investigate the effects of this template,a sample was prepared without a template via the same procedure.Through various characterization techniques,such as X-ray diffraction,scanning electron microscopy,Fourier transform infrared spectroscopy,N2 adsorption-desorption analysis,X-ray photoelectron spectroscopy,oxygen temperature programed desorption,and hydrogen temperature programed reduction,the physiochemical properties of the samples were investigated.The results showed that the sample made with a template had a larger surface area and a larger amount of adsorbed oxygen,which further illustrated that the sunflower seed shell template had a significant impact on the physiochemical properties of the samples.Furthermore,we explored the catalytic activity for nitric oxide(NO)oxidation,and studied the factors affecting it,which highlighted its potential application in automobile exhausts.