To reduce the burden of denture stomatitis and oral candidiasis,an aqueous solution containing polyhexamethylene guanidine hydrochloride(PHMGH)was investigated as an antifungal disinfectant against the leading cause o...To reduce the burden of denture stomatitis and oral candidiasis,an aqueous solution containing polyhexamethylene guanidine hydrochloride(PHMGH)was investigated as an antifungal disinfectant against the leading cause of these oral conditions,Candida albicans.The solutions formulated with concentrations ranging from 0.125 to 0.50 wt%enabled increasing disinfection at the initial 5min-contact with 72h-mature candida biofilms formed on denture liner specimens.After 10 min-contact,the solution at lower concentration has reached total fungal elimination.The results also indicated that the denture liners preserved their mechanical property after the maximum contact time with the solution at the highest tested concentration.The PHMGH aqueous solutions at 0.125 wt%could be applied to promote interim denture liner disinfection without promoting the loss of materials’mechanical property.展开更多
Exosomes derived from mesenchymal stem cells(MSCs)have demonstrated regenerative potential for cell-free bone tissue engineering,nevertheless,certain challenges,including the confined therapeutic potency of exosomes a...Exosomes derived from mesenchymal stem cells(MSCs)have demonstrated regenerative potential for cell-free bone tissue engineering,nevertheless,certain challenges,including the confined therapeutic potency of exosomes and ineffective delivery method,are still persisted.Here,we confirmed that hypoxic precondition could induce enhanced secretion of exosomes from stem cells from human exfoliated deciduous teeth(SHEDs)via comprehensive proteomics analysis,and the corresponding hypoxic exosomes(H-Exo)exhibited superior potential in promoting cellular angiogenesis and osteogenesis via the significant up-regulation in focal adhesion,VEGF signaling pathway,and thyroid hormone synthesis.Then,we developed a platform technology enabling the effective delivery of hypoxic exosomes with sustained release kinetics to irregular-shaped bone defects via injection.This platform is based on a simple adsorbing technique,where exosomes are adsorbed onto the surface of injectable porous poly(lactide-co-glycolide)(PLGA)microspheres with bioinspired polydopamine(PDA)coating(PMS-PDA microspheres).The PMS-PDA microspheres could effectively adsorb exosomes,show sustained release of H-Exo for 21 days with high bioactivity,and induce vascularized bone regeneration in 5-mm rat calvarial defect.These findings indicate that the hypoxic precondition and PMS-PDA porous microsphere-based exosome delivery are efficient in inducing tissue regeneration,hence facilitating the clinical translation of exosome-based therapy.展开更多
文摘To reduce the burden of denture stomatitis and oral candidiasis,an aqueous solution containing polyhexamethylene guanidine hydrochloride(PHMGH)was investigated as an antifungal disinfectant against the leading cause of these oral conditions,Candida albicans.The solutions formulated with concentrations ranging from 0.125 to 0.50 wt%enabled increasing disinfection at the initial 5min-contact with 72h-mature candida biofilms formed on denture liner specimens.After 10 min-contact,the solution at lower concentration has reached total fungal elimination.The results also indicated that the denture liners preserved their mechanical property after the maximum contact time with the solution at the highest tested concentration.The PHMGH aqueous solutions at 0.125 wt%could be applied to promote interim denture liner disinfection without promoting the loss of materials’mechanical property.
基金the financial support from Beijing Natural Science Foundation(7212135)National Natural Science Foundation of China(NSFC)(11972001,11972002 and 12072001).
文摘Exosomes derived from mesenchymal stem cells(MSCs)have demonstrated regenerative potential for cell-free bone tissue engineering,nevertheless,certain challenges,including the confined therapeutic potency of exosomes and ineffective delivery method,are still persisted.Here,we confirmed that hypoxic precondition could induce enhanced secretion of exosomes from stem cells from human exfoliated deciduous teeth(SHEDs)via comprehensive proteomics analysis,and the corresponding hypoxic exosomes(H-Exo)exhibited superior potential in promoting cellular angiogenesis and osteogenesis via the significant up-regulation in focal adhesion,VEGF signaling pathway,and thyroid hormone synthesis.Then,we developed a platform technology enabling the effective delivery of hypoxic exosomes with sustained release kinetics to irregular-shaped bone defects via injection.This platform is based on a simple adsorbing technique,where exosomes are adsorbed onto the surface of injectable porous poly(lactide-co-glycolide)(PLGA)microspheres with bioinspired polydopamine(PDA)coating(PMS-PDA microspheres).The PMS-PDA microspheres could effectively adsorb exosomes,show sustained release of H-Exo for 21 days with high bioactivity,and induce vascularized bone regeneration in 5-mm rat calvarial defect.These findings indicate that the hypoxic precondition and PMS-PDA porous microsphere-based exosome delivery are efficient in inducing tissue regeneration,hence facilitating the clinical translation of exosome-based therapy.
