Yolk sac tumors (YSTs) are rare malignant germ cell tumors that usually affect youngfemales. To date, there have been few studies on YSTs. We evaluated the relationship betweenclinicopathologic characteristics of pati...Yolk sac tumors (YSTs) are rare malignant germ cell tumors that usually affect youngfemales. To date, there have been few studies on YSTs. We evaluated the relationship betweenclinicopathologic characteristics of patients with ovarian YSTs and disease outcome based onSurveillance, Epidemiology, and End Results data. The K aplan-Meier method and log-rank testwere used to evaluate differences in survival rates. Data for 269 patients were analyzed. Theincidence of YSTs among ovarian germ cell tumors (OGCTs) cases was 0.4%;median patient agewas 22.0 years, and most tumors were unilateral. Patients presented with distant metastasis (37.5%),localized disease (49. 1%), and regional spread (8.9%). American Joint Committee on Cancer stagewas available for 13 patients (stage IA, n=2;stage IC, n=1;stage IA, n=1;stage IB, n=3;stageIlC, n=2;and stage IV, n=4). Survival rates at 1, 3, and 5 years were 91.0%, 84.0%, and 83.2%,respectively, for overall survival (OS) and 92.0%, 85.4%, and 84.5%, respectively, for disease-specific survival (DSS). The 5-year OS and DSS of patients with ovary tumors were 91.5% and92.9%, respectively, compared to 74.8% and 77.2%, respectively, for those with extra-ovarianspread (P<0.001 for both OS and DSS). Age >50 years was associated with shorter OS and DSS(both P<0.001), whereas no associatios of OS and DSS were observed with pathologic grade (P=0.49for OS and 0.52 for DSS). In summary, YSTs are typically unilateral, of a high grade, and localizedto the ovary;extra-ovarian spread has a poor outcome, and postmenopausal women have worseprognosis than premenopausal women.展开更多
Objective:To investigate the effects of interleukin-1beta(IL-1β) on expression of IL-8 in endometrial stromal cells(ESC) and evaluate the relationship between IL-1β and IL-8,and the significance of IL-1β in the dev...Objective:To investigate the effects of interleukin-1beta(IL-1β) on expression of IL-8 in endometrial stromal cells(ESC) and evaluate the relationship between IL-1β and IL-8,and the significance of IL-1β in the development of endometriosis.Methods:The endometrial stromal cells obtained from patient with and without endometriosis cultured within 3～5 passage were exposed to various concentrations of IL-1β.The amount of IL-8 protein was assessed by ELISA.The expression of IL-8 mRNA was determined by RT-PCR.Results:1.IL-8 protein was detected in culture supernatant of which the cells were not treated with IL-1β.The amount of IL-8 protein secretion increased obviously after stimulation with IL-1β at 1.0ng/ml for 4h and the peak of secretion was at 12h.2.Expression of IL-8 mRNA was positive in unstimulated endometrial stromal cells.However,after stromal cells were incubated with IL-1β,the intensity of expression of IL-8 mRNA was obviously increased and demonstrated a dose-and time-dependent manner.Increase of IL-8 mRNA was observed following stimulation with IL-1β for 4h,and the peak at 12h.Conclusions:IL-1β induces endometrial stromal cell of endometriosis to express IL-8 not only at transcription level but also at post-transcription level.This up-regulation is dose-and time-dependent.IL-1β may play an important role in the onset of endometriosis.【展开更多
The utility of placental growth factor(PIGF)and its receptor VEGFR-1(Flt-1)as biomarkers for cervical cancer has not been clarified yet.To address this issue,we investigated the levels of soluble PIGF(sPIGF)and solubl...The utility of placental growth factor(PIGF)and its receptor VEGFR-1(Flt-1)as biomarkers for cervical cancer has not been clarified yet.To address this issue,we investigated the levels of soluble PIGF(sPIGF)and soluble Flt-1(sFlt-1)in the serum from patients with early cervical cancer,cervical intraepithelial neoplasia(CIN)and controls in this study.sPIGF and sFlt-1 were detected in 44 preoperative patients with cervical cancer,18 cases with CIN,and 20 controls by ELISA.It was found that both sPIGF and sFlt-1 were significantly increased in the cervical cancer group as compared with those in CIN and control groups.sPIGF presented a high diagnostic ability of cervical cancer,with a sensitivity of 61.36%and a specifcity of 89.47%;and sFlt-1 with a sensitivity of 50.00%and a specifcity of 92.I 1%。Importantly,the combined use of sPIGF and sFlt-1 could increase the diagnostic rate of cervical cancer,with a sensitivity of 70.45%and a specificity of 92.11%.These results indicated that both sPIGF and sFlt-1 in circulation can serve as possible valuable diagnostic biomarkers for cervical cancer,and the combined use of them can be more valuable to diagnose the patients with early cervical cancer.展开更多
Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during pregnancy.Women with ITP who have severe thrombocytopenia...Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during pregnancy.Women with ITP who have severe thrombocytopenia are at an increased risk for life-threatening obstetric complications.Therefore,we established this consensus statement on the diagnosis and management of ITP during pregnancy(detailed information is available in the Supplementary File,http://links.lww.com7CM9/A978).展开更多
Background Post-operation pain is a very subjective phenomenon. The aim of this study was to find out the effects of acupuncture or electro-acupuncture on post-cesarean pain. Methods Sixty women, who had had spinal an...Background Post-operation pain is a very subjective phenomenon. The aim of this study was to find out the effects of acupuncture or electro-acupuncture on post-cesarean pain. Methods Sixty women, who had had spinal anesthesia during cesarean section at the Department of Obstetrics of China Medical University Hospital, were randomly assigned to the control group, the acupuncture group, and the electro-acupuncture group. After the operation, we applied subjects with acupuncture or electro-acupuncture on the bilateral acupuncture point, San Yin Jiao （Sp6）, and the patient controlled analgesia （PCA）. The first time of requesting morphine, the frequency of PCA demands in 24 hours, and the doses of PCA used were recorded double blindly. In addition, monitoring the subjects＇ vital signs, the opioid-related side effects, and the pain scores was done. Results The results showed that the acupuncture group and the electro-acupuncture group could delay the time of requesting morphine up to 10-11 minutes when compared with the control group. The total dose of PCA used within the first 24 hours was 30%-35% less in the acupuncture group and the electro-acupuncture group when compared with the control group, which was indicated in statistical significance. However, there was no significant difference between the acupuncture group and the electro-acupuncture group. The electro-acupuncture group＇s and the acupuncture group＇s pain scores were lower than the control group＇s within the first 2 hours. Both were statistically significant. However, two hours later, there were no significant differences of the visual analogue scale （VAS） scores between either of the treatment groups and the control group. Finally, the incidence of opioid-related side effects, such as dizziness, was less in the acupuncture group and electro-acupuncture group than in the control group. Conclusions This study shows that the application of acupuncture and electro-acupuncture could definitely delay the time of requesting pain relief medication after cesarean section and decrease the PCA doses used within the first 24 hours.展开更多
文摘Yolk sac tumors (YSTs) are rare malignant germ cell tumors that usually affect youngfemales. To date, there have been few studies on YSTs. We evaluated the relationship betweenclinicopathologic characteristics of patients with ovarian YSTs and disease outcome based onSurveillance, Epidemiology, and End Results data. The K aplan-Meier method and log-rank testwere used to evaluate differences in survival rates. Data for 269 patients were analyzed. Theincidence of YSTs among ovarian germ cell tumors (OGCTs) cases was 0.4%;median patient agewas 22.0 years, and most tumors were unilateral. Patients presented with distant metastasis (37.5%),localized disease (49. 1%), and regional spread (8.9%). American Joint Committee on Cancer stagewas available for 13 patients (stage IA, n=2;stage IC, n=1;stage IA, n=1;stage IB, n=3;stageIlC, n=2;and stage IV, n=4). Survival rates at 1, 3, and 5 years were 91.0%, 84.0%, and 83.2%,respectively, for overall survival (OS) and 92.0%, 85.4%, and 84.5%, respectively, for disease-specific survival (DSS). The 5-year OS and DSS of patients with ovary tumors were 91.5% and92.9%, respectively, compared to 74.8% and 77.2%, respectively, for those with extra-ovarianspread (P<0.001 for both OS and DSS). Age >50 years was associated with shorter OS and DSS(both P<0.001), whereas no associatios of OS and DSS were observed with pathologic grade (P=0.49for OS and 0.52 for DSS). In summary, YSTs are typically unilateral, of a high grade, and localizedto the ovary;extra-ovarian spread has a poor outcome, and postmenopausal women have worseprognosis than premenopausal women.
基金This research work was supported by excellent middle-aged scientist fellowship of Shandong Province(2000BB2CJAI).
文摘Objective:To investigate the effects of interleukin-1beta(IL-1β) on expression of IL-8 in endometrial stromal cells(ESC) and evaluate the relationship between IL-1β and IL-8,and the significance of IL-1β in the development of endometriosis.Methods:The endometrial stromal cells obtained from patient with and without endometriosis cultured within 3～5 passage were exposed to various concentrations of IL-1β.The amount of IL-8 protein was assessed by ELISA.The expression of IL-8 mRNA was determined by RT-PCR.Results:1.IL-8 protein was detected in culture supernatant of which the cells were not treated with IL-1β.The amount of IL-8 protein secretion increased obviously after stimulation with IL-1β at 1.0ng/ml for 4h and the peak of secretion was at 12h.2.Expression of IL-8 mRNA was positive in unstimulated endometrial stromal cells.However,after stromal cells were incubated with IL-1β,the intensity of expression of IL-8 mRNA was obviously increased and demonstrated a dose-and time-dependent manner.Increase of IL-8 mRNA was observed following stimulation with IL-1β for 4h,and the peak at 12h.Conclusions:IL-1β induces endometrial stromal cell of endometriosis to express IL-8 not only at transcription level but also at post-transcription level.This up-regulation is dose-and time-dependent.IL-1β may play an important role in the onset of endometriosis.【
基金This work was supported by the National Natural Science Foundation of China(No.81202063).
文摘The utility of placental growth factor(PIGF)and its receptor VEGFR-1(Flt-1)as biomarkers for cervical cancer has not been clarified yet.To address this issue,we investigated the levels of soluble PIGF(sPIGF)and soluble Flt-1(sFlt-1)in the serum from patients with early cervical cancer,cervical intraepithelial neoplasia(CIN)and controls in this study.sPIGF and sFlt-1 were detected in 44 preoperative patients with cervical cancer,18 cases with CIN,and 20 controls by ELISA.It was found that both sPIGF and sFlt-1 were significantly increased in the cervical cancer group as compared with those in CIN and control groups.sPIGF presented a high diagnostic ability of cervical cancer,with a sensitivity of 61.36%and a specifcity of 89.47%;and sFlt-1 with a sensitivity of 50.00%and a specifcity of 92.I 1%。Importantly,the combined use of sPIGF and sFlt-1 could increase the diagnostic rate of cervical cancer,with a sensitivity of 70.45%and a specificity of 92.11%.These results indicated that both sPIGF and sFlt-1 in circulation can serve as possible valuable diagnostic biomarkers for cervical cancer,and the combined use of them can be more valuable to diagnose the patients with early cervical cancer.
基金National Key Research and Development Program of China(No. 2017YFA0105503)National Natural Science Foundation of China(No. 81970113)+1 种基金Key Program of National Natural Science Foundation of China(No. 81730004)Beijing Natural Science Foundation(No. H2018206423)。
文摘Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during pregnancy.Women with ITP who have severe thrombocytopenia are at an increased risk for life-threatening obstetric complications.Therefore,we established this consensus statement on the diagnosis and management of ITP during pregnancy(detailed information is available in the Supplementary File,http://links.lww.com7CM9/A978).
文摘Background Post-operation pain is a very subjective phenomenon. The aim of this study was to find out the effects of acupuncture or electro-acupuncture on post-cesarean pain. Methods Sixty women, who had had spinal anesthesia during cesarean section at the Department of Obstetrics of China Medical University Hospital, were randomly assigned to the control group, the acupuncture group, and the electro-acupuncture group. After the operation, we applied subjects with acupuncture or electro-acupuncture on the bilateral acupuncture point, San Yin Jiao （Sp6）, and the patient controlled analgesia （PCA）. The first time of requesting morphine, the frequency of PCA demands in 24 hours, and the doses of PCA used were recorded double blindly. In addition, monitoring the subjects＇ vital signs, the opioid-related side effects, and the pain scores was done. Results The results showed that the acupuncture group and the electro-acupuncture group could delay the time of requesting morphine up to 10-11 minutes when compared with the control group. The total dose of PCA used within the first 24 hours was 30%-35% less in the acupuncture group and the electro-acupuncture group when compared with the control group, which was indicated in statistical significance. However, there was no significant difference between the acupuncture group and the electro-acupuncture group. The electro-acupuncture group＇s and the acupuncture group＇s pain scores were lower than the control group＇s within the first 2 hours. Both were statistically significant. However, two hours later, there were no significant differences of the visual analogue scale （VAS） scores between either of the treatment groups and the control group. Finally, the incidence of opioid-related side effects, such as dizziness, was less in the acupuncture group and electro-acupuncture group than in the control group. Conclusions This study shows that the application of acupuncture and electro-acupuncture could definitely delay the time of requesting pain relief medication after cesarean section and decrease the PCA doses used within the first 24 hours.
