Background Exposure to bisphenol A(BPA),an environmental pollutant known for its endocrine-disrupting properties,during gestation has been reported to increase the risk of fetal growth restriction(FGR)in an ovine mode...Background Exposure to bisphenol A(BPA),an environmental pollutant known for its endocrine-disrupting properties,during gestation has been reported to increase the risk of fetal growth restriction(FGR)in an ovine model of pregnancy.We hypothesized that the FGR results from the BPA-induced insufficiency and barrier dysfunction of the placenta,oxidative stress,inflammatory responses,autophagy and endoplasmic reticulum stress(ERS).However,precise mechanisms underlying the BPA-induced placental dysfunction,and subsequently,FGR,as well as the potential involvement of placental ERS in these complications,remain to be investigated.Methods In vivo experiment,16 twin-pregnant(from d 40 to 130 of gestation)Hu ewes were randomly distributed into two groups(8 ewes each).One group served as a control and received corn oil once a day,whereas the other group received BPA(5 mg/kg/d as a subcutaneous injection).In vitro study,ovine trophoblast cells(OTCs)were exposed to 4 treatments,6 replicates each.The OTCs were treated with 400μmol/L BPA,400μmol/L BPA+0.5μg/m L tunicamycin(Tm;ERS activator),400μmol/L BPA+1μmol/L 4-phenyl butyric acid(4-PBA;ERS antagonist)and DMEM/F12 complete medium(control),for 24 h.Results In vivo experiments,pregnant Hu ewes receiving the BPA from 40 to 130 days of pregnancy experienced a decrease in placental efficiency,progesterone(P4)level and fetal weight,and an increase in placental estrogen(E2)level,together with barrier dysfunctions,OS,inflammatory responses,autophagy and ERS in type A cotyledons.In vitro experiment,the OTCs exposed to BPA for 24 h showed an increase in the E2 level and related protein and gene expressions of autophagy,ERS,pro-apoptosis and inflammatory response,and a decrease in the P4 level and the related protein and gene expressions of antioxidant,anti-apoptosis and barrier function.Moreover,treating the OTCs with Tm aggravated BPA-induced dysfunction of barrier and endocrine(the increased E2 level and decreased P4 level),OS,inflammatory responses,autophagy,and ERS.However,treating the OTCs with 4-PBA reversed the counteracted effects of Tm mentioned above.Conclusions In general,the results reveal that BPA exposure can cause ERS in the ovine placenta and OTCs,and ERS induction might aggravate BPA-induced dysfunction of the placental barrier and endocrine,OS,inflammatory responses,and autophagy.These data offer novel mechanistic insights into whether ERS is involved in BPA-mediated placental dysfunction and fetal development.展开更多
This manuscript explores the biological and economic efficiency of partial dietary substitution of soybean (Glycine max) meal with cowpea (Vigna unguiculata) meal in broiler production. As a result of the shortage of ...This manuscript explores the biological and economic efficiency of partial dietary substitution of soybean (Glycine max) meal with cowpea (Vigna unguiculata) meal in broiler production. As a result of the shortage of soybean in Zimbabwe due to droughts, inadequate irrigation infrastructure, high input costs, and price controls, the authors identified an alternative to soybean meal in cowpea meal due to its availability and similar amino acid profile to soybean meal. The experiment was carried out at Cold Storage Company in Kadoma. A total of 150 unsexed day-old broiler chicks (Cobb 500) were used. All the birds were brooded in one pen during the first two weeks of age and fed with standard broiler starter mash from National Foods PVT LTD for adaptation. The birds were randomly allocated into five equal groups, each with three replicates and 10 birds per replicate at three weeks of age. Five experimental diets: T1 (100% soybean meal + 0% cowpeas meal), T2 (75% soybean meal + 25% cowpeas meal), T3 (50% soybean meal + 50% cowpeas meal), T4 (25% soybean meal + 75% cowpeas meal) and T5 (0% soybean meal + 100% cowpeas meal) were formulated to be iso-caloric and iso-nitrogenous to meet nutrient requirements of broilers according to NRC (2001). Ration formulation using maize, soybean and cowpea meal as ingredients for broiler starter (21% Crude Protein) and broiler finisher (19% Crude Protein) was done using the Pearson Square Method. Feed intake (g) and weight gain (g) were recorded weekly. Feed Conversion Ratio (FCR) and bioeconomic efficiency were calculated weekly. The experiment lasted for five weeks. The results showed that total Dry Matter Intake for Treatment 1 was the highest (3285 g), and Dry Matter Intakes for Treatments 2, 3 and 4 (3284 g, 3284 g and 3284 g) were not significantly different (P > 0.05) and Dry Matter Intake for Treatment 5 was the lowest (3282 g). Weight gains for Treatments 1 and 2 (2089 g and 2089 g) were not different (P > 0.05), Treatments 3 and 4 (2098 g and 2103 g) were not different (P > 0.05) and Treatment 5 had the lowest gain of 1990 g. Feed Conversion Ratio for Treatments 1 and 2 (1.572 and 1.572) was not significantly different (P > 0.05), Treatments 3 and 4 (1.565 and 1.562) were not different (P > 0.05) and Treatment 5 had the highest FCR (1.64). The bioeconomic efficiency results were significantly different (P < 0.05) for Treatments 1, 2, 3, 4 and 5 (42.16, 41.93, 41.46, 41.13 and 42.66, respectively). Based on the analyses and results, the authors concluded that cowpea meal prepared from boiled cowpea grain can be used to replace soybean meal in broiler diets at 50% and 75% inclusion, respectively. This study is unique and interesting and suitable for acceptance in this journal because it seeks to provide an alternative to feeds for the ever-growing broiler industry in Zimbabwe and the rest of the world.展开更多
This manuscript explores the biological and economic efficiency of partial dietary substitution of soybean (Glycine max) meal with cowpea (Vigna unguiculata) meal in broiler production. As a result of the shortage of ...This manuscript explores the biological and economic efficiency of partial dietary substitution of soybean (Glycine max) meal with cowpea (Vigna unguiculata) meal in broiler production. As a result of the shortage of soybean in Zimbabwe due to droughts, inadequate irrigation infrastructure, high input costs, and price controls, the authors identified an alternative to soybean meal in cowpea meal due to its availability and similar amino acid profile to soybean meal. The experiment was carried out at Cold Storage Company in Kadoma. A total of 150 unsexed day-old broiler chicks (Cobb 500) were used. All the birds were brooded in one pen during the first two weeks of age and fed with standard broiler starter mash from National Foods PVT LTD for adaptation. The birds were randomly allocated into five equal groups, each with three replicates and 10 birds per replicate at three weeks of age. Five experimental diets: T1 (100% soybean meal + 0% cowpeas meal), T2 (75% soybean meal + 25% cowpeas meal), T3 (50% soybean meal + 50% cowpeas meal), T4 (25% soybean meal + 75% cowpeas meal) and T5 (0% soybean meal + 100% cowpeas meal) were formulated to be iso-caloric and iso-nitrogenous to meet nutrient requirements of broilers according to NRC (2001). Ration formulation using maize, soybean and cowpea meal as ingredients for broiler starter (21% Crude Protein) and broiler finisher (19% Crude Protein) was done using the Pearson Square Method. Feed intake (g) and weight gain (g) were recorded weekly. Feed Conversion Ratio (FCR) and bioeconomic efficiency were calculated weekly. The experiment lasted for five weeks. The results showed that total Dry Matter Intake for Treatment 1 was the highest (3285 g), and Dry Matter Intakes for Treatments 2, 3 and 4 (3284 g, 3284 g and 3284 g) were not significantly different (P > 0.05) and Dry Matter Intake for Treatment 5 was the lowest (3282 g). Weight gains for Treatments 1 and 2 (2089 g and 2089 g) were not different (P > 0.05), Treatments 3 and 4 (2098 g and 2103 g) were not different (P > 0.05) and Treatment 5 had the lowest gain of 1990 g. Feed Conversion Ratio for Treatments 1 and 2 (1.572 and 1.572) was not significantly different (P > 0.05), Treatments 3 and 4 (1.565 and 1.562) were not different (P > 0.05) and Treatment 5 had the highest FCR (1.64). The bioeconomic efficiency results were significantly different (P < 0.05) for Treatments 1, 2, 3, 4 and 5 (42.16, 41.93, 41.46, 41.13 and 42.66, respectively). Based on the analyses and results, the authors concluded that cowpea meal prepared from boiled cowpea grain can be used to replace soybean meal in broiler diets at 50% and 75% inclusion, respectively. This study is unique and interesting and suitable for acceptance in this journal because it seeks to provide an alternative to feeds for the ever-growing broiler industry in Zimbabwe and the rest of the world.展开更多
Opuntia ficus-indica(L.)Miller is a CAM(crassulacean acid metabolism)plant with an extraordinary capacity to adapt to drought stress by its ability to fix atmospheric CO_(2) at nighttime,store a significant amount of ...Opuntia ficus-indica(L.)Miller is a CAM(crassulacean acid metabolism)plant with an extraordinary capacity to adapt to drought stress by its ability to fix atmospheric CO_(2) at nighttime,store a significant amount of water in cladodes,and reduce root growth.Plants that grow in moisture-stress conditions with thick and less fine root hairs have a strong symbiosis with arbuscular mycorrhizal fungi(AMF)to adapt to drought stress.Water stress can limit plant growth and biomass production,which can be rehabilitated by AMF association through improved physiological performance.The objective of this study was to investigate the effects of AMF inoculations and variable soil water levels on the biomass,photosynthesis,and water use efficiency of the spiny and spineless O.ficus-indica.The experiment was conducted in a greenhouse with a full factorial experiment using O.ficus-indica type(spiny or spineless),AMF(presence or absence),and four soil water available(SWA)treatments through seven replications.Water treatments applied were 0%–25%SWA(T1),25%–50%SWA(T2),50%–75%SWA(T3),and 75%–100%SWA(T4).Drought stress reduced biomass and cladode growth,while AMF colonization significantly increased the biomass production with significant changes in the physiological performance of O.ficus-indica.AMF presence significantly increased biomass of both O.ficus-indica plant types through improved growth,photosynthetic water use efficiency,and photosynthesis.The presence of spines on the surface of cladodes significantly reduced the rate of photosynthesis and photosynthetic water use efficiency.Net photosynthesis,photosynthetic water use efficiency,transpiration,and stomatal conductance rate significantly decreased with increased drought stress.Under drought stress,some planted mother cladodes with the absence of AMF have not established daughter cladodes,whereas AMF-inoculated mother cladodes fully established daughter cladodes.AMF root colonization significantly increased with the decrease of SWA.AMF caused an increase in biomass production,increased tolerance to drought stress,and improved photosynthesis and water use efficiency performance of O.ficus-indica.The potential of O.ficus-indica to adapt to drought stress is controlled by the morpho-physiological performance related to AMF association.展开更多
The aim of the study was to assess hygienic production practices and to evaluate microbial quality of raw cows’ milk in Cheha district of Gurage Zone, Southern Ethiopia. The study was conducted by interviewing 180 sm...The aim of the study was to assess hygienic production practices and to evaluate microbial quality of raw cows’ milk in Cheha district of Gurage Zone, Southern Ethiopia. The study was conducted by interviewing 180 smallholder milk producers from two agro-ecologies (highland and midland) and by collecting 40 milk samples from milk producers, small shops, cafes and consumers for microbial quality analysis. The result indicated that majority of the respondents (96.7%) did not have cow barn and milking area, 57.3% of them clean their barn once and more than once a week. Moreover, milking without hand washing, absence of udder washing, failure to use towel to clean the udder, and use of plastic pail as milking equipment were practiced by 33.9%, 52.2%, 74.4% and 95.6% of the respondents, respectively. Furthermore, higher number of respondents (69.4%) used river water without proper treatment as a major source of water for cleaning purpose and majority of them fail to regularly clean utensils with detergents. Concerning microbial quality, overall mean total bacterial counts (TBC), Coliform counts (CC) and pH values of milk from current finding were 5.675 ± 0.016, 4.414 ± 0.017 log10 cfu/ml, and 5.963 ± 0.081, respectively. In general, overall quality of milk produced as well as marketed in the study area was poor. These were justified from poor hygienic practices and high values of TBC and CC that were significantly higher than the acceptable limits set by Ethiopian Standards for unpasteurized milk. Therefore, adequate sanitary and control measures should be taken at all stages from production to consumer level to produce and supply wholesome milk.展开更多
Key issues of concern regarding the environmental impacts of livestock on grazing land are their effects on soil, water quality, and biodiversity. This study was carried out to determine how grazing intensity influenc...Key issues of concern regarding the environmental impacts of livestock on grazing land are their effects on soil, water quality, and biodiversity. This study was carried out to determine how grazing intensity influences soil physical and chemical properties and occurrence of herbaceous plant species in dambo wetlands. Three categories of grazing intensity were selected from communal, small scale commercial and large scale commercial land. Dambos from the large scale commercial land functioned as the control. Data analysis included ANOVA and multivariate tests from CANOCO. There were significantly negative changes to soil nutrient status in communal dambos though with a higher number of rare taxa. Sodium, phosphorous, pH and infiltration rate were significant determinants of plant species occurrence. Overgrazing is threatening the productivity, stability, and ecological functioning of dambo soils in communal Zimbabwe. These dambos also require special conservation and management priorities as they contain a large number of rare plant species.展开更多
Camel milk is unique because it is not easily processed into different dairy products that are common for cow milk. It lacks β-lactoglobulin and has low κ-casein that hinders processing of camel milk into different ...Camel milk is unique because it is not easily processed into different dairy products that are common for cow milk. It lacks β-lactoglobulin and has low κ-casein that hinders processing of camel milk into different dairy products. Hence it needs different processing methods and technologies. To this effect attempts were made to manufacture dairy products such as feta cheese, soft brined cheese, and fermented milk from camel milk that had been produced at laboratory level. Camel milk powder has also been manufactures and marketed in Middle East and beyond. Currently there are remarkable progresses in camel dairy technologies that paved the way for production of different camel dairy products at small, medium and large scale. A number of experiments were conducted on camel milk processing technologies from preservation of camel milk using lactoperoxidase system activation using hydrogen peroxide (H2O2) and thiocyanate as well as by H2O2 producing lactic acid bacteria (LAB) to metagenomic characterization of LAB isolated from spontaneously fermented camel milk. Therefore, this review paper is going to present current status of camel dairy technologies in Ethiopia.展开更多
Cricket is widely recognized in Indonesia especially in Java island, and commonly kept by village farmers for food and feed. In general, crickets has very short life cycle （60-70 d）, with three weeks of laying perio...Cricket is widely recognized in Indonesia especially in Java island, and commonly kept by village farmers for food and feed. In general, crickets has very short life cycle （60-70 d）, with three weeks of laying period and egg production within the range of 200-1,500 eggs/female. The female has capability for multiple mating with several males and therefore produces more fertile eggs. Serial studies including experiments on laboratory and field observation of local crickets had been conducted to evaluate the farming practices and reproductive performance of three local species （Gryllus spp., Gryllus mitratus and Gryllus testaceus） which were given local feeds--Manihot esculenta and Carica papaya leaves. The experiment was conducted in randomized block design with feed as the treatments and species as the block. Cliring crickets （G. mitratus） fed with M. esculenta leaves revealed the highest total egg production （1,478.22 eggs）, with daily egg production of 32.85 eggs/d, the longest laying period （45 d）, the lowest feed intake （0.28 g/head） and feed conversion （0.93 mg/egg）, compared to Cendawang crickets （G. testaceus） and Gryllus spp.. The revenue and cost （R/C） ratio was 1.4, indicating that small scale cricket enterprise could offer significant profit and could be chosen as an alternative activities for earning regular income.展开更多
Tetragonula is one of stingless bees genus widely spread in Indo-Pacific with various species,including Indonesia.This genus is also found in West Halmahera,but it does not have a unique character as the marker,so it ...Tetragonula is one of stingless bees genus widely spread in Indo-Pacific with various species,including Indonesia.This genus is also found in West Halmahera,but it does not have a unique character as the marker,so it is difficult to identify.The best way to identify is by measuring the body part from each individual.This research was conducted to analyze morphometry to know the diversity of stingless bee in West Halmahera.Three hundred thirty-two(332)stingless bees from 51 colonies of three different species were used,namely Tetragonula clypearis,40 colonies(252 bees),T.sapiens,10 colonies(72 bees),and T.biroi,one colony(eight bees).Morphometry of stingless bees were analyzed based on principal component analysis(PCA),visualized into scree plot,score plot,loading plot and biplot.The results showed that only two principal components(PCs)on eigenvalue have a score more than one with the total proportions are 85.8%.All the species were not correlated because they appeared in different quadrants.Morphometry variables have a positive correlation with T.sapiens,but negative correlation with T.clypearies,while T.biroi has a weak correlation with the variables of T.sapiens.T.sapiens can only be found in the highlands,T.clypearis is mostly found in medium elevations but can also be found in highlands,while T.biroi can only be found in lowlands.展开更多
Cattle are central to the lives and diverse cultures of African people.It has played a crucial role in providing valuable protein for billions of households and sources of income and employment for producers and other...Cattle are central to the lives and diverse cultures of African people.It has played a crucial role in providing valuable protein for billions of households and sources of income and employment for producers and other actors in the livestock value chains.The long-term natural selection of African cattle typically signals signatures in the genome,contributes to high genetic differentiations across breeds.This has enabled them to develop unique adaptive traits to cope with inadequate feed supply,high temperatures,high internal and external parasites,and diseases.However,these unique cattle genetic resources are threatened by indiscriminate cross-breeding,breed replacements with exotic cosmopolitan breeds,and climate change pressures.Although there are no functional genomics studies,recent advancements in genotyping and sequencing technologies have identified and annotated limited functional genes and causal variants associated with unique adaptive and economical traits of African cattle populations.These genome-wide variants serve as candidates for breed improvement and support conservation efforts for endangered cattle breeds against future climate changes.Therefore,this review plans to collate comprehensive information on the identified selection footprints to support genomic studies in African cattle to confirm the validity of the results and provide a framework for further genetic association and QTL fine mapping studies.展开更多
The study was conducted to asses handling, processing, utilization, marketing as well as other socio-economic benefits of traditional cheese varieties making and to identify major constraints of traditional cheese var...The study was conducted to asses handling, processing, utilization, marketing as well as other socio-economic benefits of traditional cheese varieties making and to identify major constraints of traditional cheese varieties production in Awabal, Gozamin, Machakel and Dejen districts of Eastern Gojjam. A cross-sectional survey was conducted to collect data. A total of 240 smallholder dairy producers (60 households from each district) were individually interviewed using a semi-structured questionnaire. The major traditional cheese varieties produced in the study areas include Ayib, Metata, Hazo and Zureshekefign. Among these, three of them (Ayib, Metata and Hazo), are dominant. Respondents indicated that Metata cheese variety has high nutritional values and cure seven types of disease namely malaria and yellow fever, depressed appetite;Ejeseb (stomach upset), metabolic disorder (bloating), gastro-intestinal parasites, prevent nausea and digestion upset. The respondents reported twenty kinds of spices blended at one time during Metata cheese preparation and five kinds of spices commonly added into Ayib during Ayib making. Various types of herbs and plant species are used for smoking and washing of utensils during production of traditional cheese varieties in the study areas. The major marketing constraints of traditional cheese varieties were absence of sufficient buyer in the market, traditional taboos to sell cheese varieties in an open market, seasonal demand and supply, distance to the market, transportation access and quality. The major socio-economic benefits of traditional cheese varieties were to fulfill household necessities, sources of additional income, to purchase dairy inputs and for children school fee. Further scientific study needs to be conducted in order to confirm nutritional and medicinal properties of Metata cheese variety. Therefore the bio-active components of these spices and their effect on shelf life, nutritional value, medicinal uses and other attributes on traditional cheese varieties needed further investigation.展开更多
Previous studies have revealed that dietary N-carbamylglutamate(NCG)or L-arginine(Arg)improves small intestinal integrity and immune function in suckling Hu lambs that have experienced intrauterine growth retardation(...Previous studies have revealed that dietary N-carbamylglutamate(NCG)or L-arginine(Arg)improves small intestinal integrity and immune function in suckling Hu lambs that have experienced intrauterine growth retardation(IUGR).Whether these nutrients alter redox status and apoptosis in the colon of IUGR lambs is still unknown.This study,therefore,aimed at investigating whether dietary supplementation of Arg or NCG alters colonic redox status,apoptosis and endoplasmic reticulum(ER)stress and the underlying mechanism of these alterations in IUGR suckling Hu lambs.Forty-eight 7-d old Hu lambs,including 12 with normal birth weight(4.25±0.14 kg)and 36 with IUGR(3.01±0.12 kg),were assigned to 4 treatment groups(n=12 each;6 males and 6 females)for 3 weeks.The treatment groups were control(CON),IUGR,IUGR+Arg and IUGR+NCG.Relative to IUGR lambs,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)content,as well as proliferation index,were higher(P<0.05)whereas reactive oxygen species(ROS),malondialdehyde(MDA)levels and apoptotic cell numbers were lower(P<0.05)in colonic tissue for both IUGR+Arg and NCG lambs.Both m RNA and protein levels of C/EBP homologous protein 10(CHOP10),B-cell lymphoma/leukaemia 2(Bcl-2)-associated X protein(Bax),apoptosis antigen 1(Fas),activating transcription factor 6(ATF6),caspase 3,and glucose-regulated protein 78(GRP78)were lower(P<0.05)while glutathione peroxidase 1(GPx1),Bcl-2 and catalase(CAT)levels were higher(P<0.05)in colonic tissue for IUGR+Arg and IUGR+NCG lambs compared with IUGR lambs.Based on our results,dietary NCG or Arg supplementation can improve colonic redox status and suppress apoptosis via death receptor-dependent,mitochondrial and ER stress pathways in IUGR suckling lambs.展开更多
This study aimed to investigate the effects of dietary supplementation of underfed Hu ewes from d 35 to110 of gestation with either rumen-protected L-arginine(RP-Arg)or N-carbamylglutamate(NCG)on placental amino acid(...This study aimed to investigate the effects of dietary supplementation of underfed Hu ewes from d 35 to110 of gestation with either rumen-protected L-arginine(RP-Arg)or N-carbamylglutamate(NCG)on placental amino acid(AA)transport,angiogenic gene expression,and steroid anabolism.On d 35 of gestation,32 Hu ewes carrying twin fetuses were randomly divided into four treatment groups,each consisting of eight ewes,and were fed the following diets:A diet providing 100%of NRC’s nutrient requirements for pregnant ewes(CON);A diet providing 50%of NRC’s nutrient requirements for pregnant ewes(RES);RES diet plus 5 g/d NCG(RES+NCG);or RES diet plus 20 g/d RP-Arg(RES+ARG).On the d 110 of pregnancy,blood samples were taken from the mother,and samples were collected from type A cotyledons(COT;the fetal portions of the placenta).The levels of 17β-estradiol and progesterone in the maternal serum and both the capillary area density(CAD)and capillary surface density(CSD)in type A COT were decreased in response to Arg or NCG supplementation when compared to the RES group.The concentrations of arginine,leucine,putrescine and spermidine in type A COT were higher(P<0.05)in the RES+ARG or RES+NCG group than in the RES group.The mRNA expression levels of inducible nitric oxide synthase(iNOS)and solute carrier family 15,member 1(SLC15A1)were increased(P<0.05)while those of progesterone receptor(PGR)and fibroblast growth factor 2(FGF2)were decreased in type A COT by supplementation with either NCG or RP-Arg compared to the RES group.The results suggest that providing underfed pregnant ewes from d 35 to 110 of gestation with a diet supplemented with NCG or RP-Arg improves placental AA transport,and reduces the expression of angiogenic growth factor genes and steroid anabolism,leading to better fetal development.展开更多
The aquafeed industry continues to expand in response to the rapidly growing aquaculture sector.However,the identification of alternative protein sources in aquatic animal diets to replace conventional sources due to ...The aquafeed industry continues to expand in response to the rapidly growing aquaculture sector.However,the identification of alternative protein sources in aquatic animal diets to replace conventional sources due to cost and sustainability issues remains a major challenge.Recently,insects have shown tremendous results as potential replacers of fishmeal in aquafeed.The present study aimed to review the utilization of insects in aquafeeds and their effects on aquatic animals’growth and feed utilization,immune response and disease resistance,and fish flesh quality and safety.While many insect species have been investigated in aquaculture,the black soldier fly(Hermetia illucens),and the mealworm(Tenebrio molitor)are the most studied and most promising insects to replace fishmeal in aquafeed.Generally,insect rearing conditions and biomass processing methods may affect the product’s nutritional composition,digestibility,shelf life and required insect inclusion level by aquatic animals.Also,insect-recommended inclusion levels for aquatic animals vary depending on the insect species used,biomass processing method,and test organism.Overall,while an appropriate inclusion level of insects in aquafeed provides several nutritional and health benefits to aquatic animals,more studies are needed to establish optimum requirements levels for different aquaculture species at different stages of development and under different culture systems.展开更多
基金supported by the fund for the National 14th Five-Year Plan Key Research and Development Program(2021YFD1600702)XPCC Agricultural Science and Technology Innovation Project(NCG202232)the Top Talents Award Plan of Yangzhou University(2020)。
文摘Background Exposure to bisphenol A(BPA),an environmental pollutant known for its endocrine-disrupting properties,during gestation has been reported to increase the risk of fetal growth restriction(FGR)in an ovine model of pregnancy.We hypothesized that the FGR results from the BPA-induced insufficiency and barrier dysfunction of the placenta,oxidative stress,inflammatory responses,autophagy and endoplasmic reticulum stress(ERS).However,precise mechanisms underlying the BPA-induced placental dysfunction,and subsequently,FGR,as well as the potential involvement of placental ERS in these complications,remain to be investigated.Methods In vivo experiment,16 twin-pregnant(from d 40 to 130 of gestation)Hu ewes were randomly distributed into two groups(8 ewes each).One group served as a control and received corn oil once a day,whereas the other group received BPA(5 mg/kg/d as a subcutaneous injection).In vitro study,ovine trophoblast cells(OTCs)were exposed to 4 treatments,6 replicates each.The OTCs were treated with 400μmol/L BPA,400μmol/L BPA+0.5μg/m L tunicamycin(Tm;ERS activator),400μmol/L BPA+1μmol/L 4-phenyl butyric acid(4-PBA;ERS antagonist)and DMEM/F12 complete medium(control),for 24 h.Results In vivo experiments,pregnant Hu ewes receiving the BPA from 40 to 130 days of pregnancy experienced a decrease in placental efficiency,progesterone(P4)level and fetal weight,and an increase in placental estrogen(E2)level,together with barrier dysfunctions,OS,inflammatory responses,autophagy and ERS in type A cotyledons.In vitro experiment,the OTCs exposed to BPA for 24 h showed an increase in the E2 level and related protein and gene expressions of autophagy,ERS,pro-apoptosis and inflammatory response,and a decrease in the P4 level and the related protein and gene expressions of antioxidant,anti-apoptosis and barrier function.Moreover,treating the OTCs with Tm aggravated BPA-induced dysfunction of barrier and endocrine(the increased E2 level and decreased P4 level),OS,inflammatory responses,autophagy,and ERS.However,treating the OTCs with 4-PBA reversed the counteracted effects of Tm mentioned above.Conclusions In general,the results reveal that BPA exposure can cause ERS in the ovine placenta and OTCs,and ERS induction might aggravate BPA-induced dysfunction of the placental barrier and endocrine,OS,inflammatory responses,and autophagy.These data offer novel mechanistic insights into whether ERS is involved in BPA-mediated placental dysfunction and fetal development.
文摘This manuscript explores the biological and economic efficiency of partial dietary substitution of soybean (Glycine max) meal with cowpea (Vigna unguiculata) meal in broiler production. As a result of the shortage of soybean in Zimbabwe due to droughts, inadequate irrigation infrastructure, high input costs, and price controls, the authors identified an alternative to soybean meal in cowpea meal due to its availability and similar amino acid profile to soybean meal. The experiment was carried out at Cold Storage Company in Kadoma. A total of 150 unsexed day-old broiler chicks (Cobb 500) were used. All the birds were brooded in one pen during the first two weeks of age and fed with standard broiler starter mash from National Foods PVT LTD for adaptation. The birds were randomly allocated into five equal groups, each with three replicates and 10 birds per replicate at three weeks of age. Five experimental diets: T1 (100% soybean meal + 0% cowpeas meal), T2 (75% soybean meal + 25% cowpeas meal), T3 (50% soybean meal + 50% cowpeas meal), T4 (25% soybean meal + 75% cowpeas meal) and T5 (0% soybean meal + 100% cowpeas meal) were formulated to be iso-caloric and iso-nitrogenous to meet nutrient requirements of broilers according to NRC (2001). Ration formulation using maize, soybean and cowpea meal as ingredients for broiler starter (21% Crude Protein) and broiler finisher (19% Crude Protein) was done using the Pearson Square Method. Feed intake (g) and weight gain (g) were recorded weekly. Feed Conversion Ratio (FCR) and bioeconomic efficiency were calculated weekly. The experiment lasted for five weeks. The results showed that total Dry Matter Intake for Treatment 1 was the highest (3285 g), and Dry Matter Intakes for Treatments 2, 3 and 4 (3284 g, 3284 g and 3284 g) were not significantly different (P > 0.05) and Dry Matter Intake for Treatment 5 was the lowest (3282 g). Weight gains for Treatments 1 and 2 (2089 g and 2089 g) were not different (P > 0.05), Treatments 3 and 4 (2098 g and 2103 g) were not different (P > 0.05) and Treatment 5 had the lowest gain of 1990 g. Feed Conversion Ratio for Treatments 1 and 2 (1.572 and 1.572) was not significantly different (P > 0.05), Treatments 3 and 4 (1.565 and 1.562) were not different (P > 0.05) and Treatment 5 had the highest FCR (1.64). The bioeconomic efficiency results were significantly different (P < 0.05) for Treatments 1, 2, 3, 4 and 5 (42.16, 41.93, 41.46, 41.13 and 42.66, respectively). Based on the analyses and results, the authors concluded that cowpea meal prepared from boiled cowpea grain can be used to replace soybean meal in broiler diets at 50% and 75% inclusion, respectively. This study is unique and interesting and suitable for acceptance in this journal because it seeks to provide an alternative to feeds for the ever-growing broiler industry in Zimbabwe and the rest of the world.
文摘This manuscript explores the biological and economic efficiency of partial dietary substitution of soybean (Glycine max) meal with cowpea (Vigna unguiculata) meal in broiler production. As a result of the shortage of soybean in Zimbabwe due to droughts, inadequate irrigation infrastructure, high input costs, and price controls, the authors identified an alternative to soybean meal in cowpea meal due to its availability and similar amino acid profile to soybean meal. The experiment was carried out at Cold Storage Company in Kadoma. A total of 150 unsexed day-old broiler chicks (Cobb 500) were used. All the birds were brooded in one pen during the first two weeks of age and fed with standard broiler starter mash from National Foods PVT LTD for adaptation. The birds were randomly allocated into five equal groups, each with three replicates and 10 birds per replicate at three weeks of age. Five experimental diets: T1 (100% soybean meal + 0% cowpeas meal), T2 (75% soybean meal + 25% cowpeas meal), T3 (50% soybean meal + 50% cowpeas meal), T4 (25% soybean meal + 75% cowpeas meal) and T5 (0% soybean meal + 100% cowpeas meal) were formulated to be iso-caloric and iso-nitrogenous to meet nutrient requirements of broilers according to NRC (2001). Ration formulation using maize, soybean and cowpea meal as ingredients for broiler starter (21% Crude Protein) and broiler finisher (19% Crude Protein) was done using the Pearson Square Method. Feed intake (g) and weight gain (g) were recorded weekly. Feed Conversion Ratio (FCR) and bioeconomic efficiency were calculated weekly. The experiment lasted for five weeks. The results showed that total Dry Matter Intake for Treatment 1 was the highest (3285 g), and Dry Matter Intakes for Treatments 2, 3 and 4 (3284 g, 3284 g and 3284 g) were not significantly different (P > 0.05) and Dry Matter Intake for Treatment 5 was the lowest (3282 g). Weight gains for Treatments 1 and 2 (2089 g and 2089 g) were not different (P > 0.05), Treatments 3 and 4 (2098 g and 2103 g) were not different (P > 0.05) and Treatment 5 had the lowest gain of 1990 g. Feed Conversion Ratio for Treatments 1 and 2 (1.572 and 1.572) was not significantly different (P > 0.05), Treatments 3 and 4 (1.565 and 1.562) were not different (P > 0.05) and Treatment 5 had the highest FCR (1.64). The bioeconomic efficiency results were significantly different (P < 0.05) for Treatments 1, 2, 3, 4 and 5 (42.16, 41.93, 41.46, 41.13 and 42.66, respectively). Based on the analyses and results, the authors concluded that cowpea meal prepared from boiled cowpea grain can be used to replace soybean meal in broiler diets at 50% and 75% inclusion, respectively. This study is unique and interesting and suitable for acceptance in this journal because it seeks to provide an alternative to feeds for the ever-growing broiler industry in Zimbabwe and the rest of the world.
基金supported by the Ethiopian Ministry of Education.
文摘Opuntia ficus-indica(L.)Miller is a CAM(crassulacean acid metabolism)plant with an extraordinary capacity to adapt to drought stress by its ability to fix atmospheric CO_(2) at nighttime,store a significant amount of water in cladodes,and reduce root growth.Plants that grow in moisture-stress conditions with thick and less fine root hairs have a strong symbiosis with arbuscular mycorrhizal fungi(AMF)to adapt to drought stress.Water stress can limit plant growth and biomass production,which can be rehabilitated by AMF association through improved physiological performance.The objective of this study was to investigate the effects of AMF inoculations and variable soil water levels on the biomass,photosynthesis,and water use efficiency of the spiny and spineless O.ficus-indica.The experiment was conducted in a greenhouse with a full factorial experiment using O.ficus-indica type(spiny or spineless),AMF(presence or absence),and four soil water available(SWA)treatments through seven replications.Water treatments applied were 0%–25%SWA(T1),25%–50%SWA(T2),50%–75%SWA(T3),and 75%–100%SWA(T4).Drought stress reduced biomass and cladode growth,while AMF colonization significantly increased the biomass production with significant changes in the physiological performance of O.ficus-indica.AMF presence significantly increased biomass of both O.ficus-indica plant types through improved growth,photosynthetic water use efficiency,and photosynthesis.The presence of spines on the surface of cladodes significantly reduced the rate of photosynthesis and photosynthetic water use efficiency.Net photosynthesis,photosynthetic water use efficiency,transpiration,and stomatal conductance rate significantly decreased with increased drought stress.Under drought stress,some planted mother cladodes with the absence of AMF have not established daughter cladodes,whereas AMF-inoculated mother cladodes fully established daughter cladodes.AMF root colonization significantly increased with the decrease of SWA.AMF caused an increase in biomass production,increased tolerance to drought stress,and improved photosynthesis and water use efficiency performance of O.ficus-indica.The potential of O.ficus-indica to adapt to drought stress is controlled by the morpho-physiological performance related to AMF association.
文摘The aim of the study was to assess hygienic production practices and to evaluate microbial quality of raw cows’ milk in Cheha district of Gurage Zone, Southern Ethiopia. The study was conducted by interviewing 180 smallholder milk producers from two agro-ecologies (highland and midland) and by collecting 40 milk samples from milk producers, small shops, cafes and consumers for microbial quality analysis. The result indicated that majority of the respondents (96.7%) did not have cow barn and milking area, 57.3% of them clean their barn once and more than once a week. Moreover, milking without hand washing, absence of udder washing, failure to use towel to clean the udder, and use of plastic pail as milking equipment were practiced by 33.9%, 52.2%, 74.4% and 95.6% of the respondents, respectively. Furthermore, higher number of respondents (69.4%) used river water without proper treatment as a major source of water for cleaning purpose and majority of them fail to regularly clean utensils with detergents. Concerning microbial quality, overall mean total bacterial counts (TBC), Coliform counts (CC) and pH values of milk from current finding were 5.675 ± 0.016, 4.414 ± 0.017 log10 cfu/ml, and 5.963 ± 0.081, respectively. In general, overall quality of milk produced as well as marketed in the study area was poor. These were justified from poor hygienic practices and high values of TBC and CC that were significantly higher than the acceptable limits set by Ethiopian Standards for unpasteurized milk. Therefore, adequate sanitary and control measures should be taken at all stages from production to consumer level to produce and supply wholesome milk.
文摘Key issues of concern regarding the environmental impacts of livestock on grazing land are their effects on soil, water quality, and biodiversity. This study was carried out to determine how grazing intensity influences soil physical and chemical properties and occurrence of herbaceous plant species in dambo wetlands. Three categories of grazing intensity were selected from communal, small scale commercial and large scale commercial land. Dambos from the large scale commercial land functioned as the control. Data analysis included ANOVA and multivariate tests from CANOCO. There were significantly negative changes to soil nutrient status in communal dambos though with a higher number of rare taxa. Sodium, phosphorous, pH and infiltration rate were significant determinants of plant species occurrence. Overgrazing is threatening the productivity, stability, and ecological functioning of dambo soils in communal Zimbabwe. These dambos also require special conservation and management priorities as they contain a large number of rare plant species.
文摘Camel milk is unique because it is not easily processed into different dairy products that are common for cow milk. It lacks β-lactoglobulin and has low κ-casein that hinders processing of camel milk into different dairy products. Hence it needs different processing methods and technologies. To this effect attempts were made to manufacture dairy products such as feta cheese, soft brined cheese, and fermented milk from camel milk that had been produced at laboratory level. Camel milk powder has also been manufactures and marketed in Middle East and beyond. Currently there are remarkable progresses in camel dairy technologies that paved the way for production of different camel dairy products at small, medium and large scale. A number of experiments were conducted on camel milk processing technologies from preservation of camel milk using lactoperoxidase system activation using hydrogen peroxide (H2O2) and thiocyanate as well as by H2O2 producing lactic acid bacteria (LAB) to metagenomic characterization of LAB isolated from spontaneously fermented camel milk. Therefore, this review paper is going to present current status of camel dairy technologies in Ethiopia.
文摘Cricket is widely recognized in Indonesia especially in Java island, and commonly kept by village farmers for food and feed. In general, crickets has very short life cycle （60-70 d）, with three weeks of laying period and egg production within the range of 200-1,500 eggs/female. The female has capability for multiple mating with several males and therefore produces more fertile eggs. Serial studies including experiments on laboratory and field observation of local crickets had been conducted to evaluate the farming practices and reproductive performance of three local species （Gryllus spp., Gryllus mitratus and Gryllus testaceus） which were given local feeds--Manihot esculenta and Carica papaya leaves. The experiment was conducted in randomized block design with feed as the treatments and species as the block. Cliring crickets （G. mitratus） fed with M. esculenta leaves revealed the highest total egg production （1,478.22 eggs）, with daily egg production of 32.85 eggs/d, the longest laying period （45 d）, the lowest feed intake （0.28 g/head） and feed conversion （0.93 mg/egg）, compared to Cendawang crickets （G. testaceus） and Gryllus spp.. The revenue and cost （R/C） ratio was 1.4, indicating that small scale cricket enterprise could offer significant profit and could be chosen as an alternative activities for earning regular income.
文摘Tetragonula is one of stingless bees genus widely spread in Indo-Pacific with various species,including Indonesia.This genus is also found in West Halmahera,but it does not have a unique character as the marker,so it is difficult to identify.The best way to identify is by measuring the body part from each individual.This research was conducted to analyze morphometry to know the diversity of stingless bee in West Halmahera.Three hundred thirty-two(332)stingless bees from 51 colonies of three different species were used,namely Tetragonula clypearis,40 colonies(252 bees),T.sapiens,10 colonies(72 bees),and T.biroi,one colony(eight bees).Morphometry of stingless bees were analyzed based on principal component analysis(PCA),visualized into scree plot,score plot,loading plot and biplot.The results showed that only two principal components(PCs)on eigenvalue have a score more than one with the total proportions are 85.8%.All the species were not correlated because they appeared in different quadrants.Morphometry variables have a positive correlation with T.sapiens,but negative correlation with T.clypearies,while T.biroi has a weak correlation with the variables of T.sapiens.T.sapiens can only be found in the highlands,T.clypearis is mostly found in medium elevations but can also be found in highlands,while T.biroi can only be found in lowlands.
基金The authors are grateful for the financial support by the Agricultural Science and Technology Innovation Program,China(CAAS-ASTIP-2014-LIHPS-01)the China Agriculture Research System of MOF and MARA(CARS-37)+1 种基金the Foundation for Innovation,Groups of Basic Research in Gansu Province,China(20JR5RA580)the Key Research and Development Programs of Science and Technology of Gansu Province,China(20YF8WA031)are duly acknowledged.
文摘Cattle are central to the lives and diverse cultures of African people.It has played a crucial role in providing valuable protein for billions of households and sources of income and employment for producers and other actors in the livestock value chains.The long-term natural selection of African cattle typically signals signatures in the genome,contributes to high genetic differentiations across breeds.This has enabled them to develop unique adaptive traits to cope with inadequate feed supply,high temperatures,high internal and external parasites,and diseases.However,these unique cattle genetic resources are threatened by indiscriminate cross-breeding,breed replacements with exotic cosmopolitan breeds,and climate change pressures.Although there are no functional genomics studies,recent advancements in genotyping and sequencing technologies have identified and annotated limited functional genes and causal variants associated with unique adaptive and economical traits of African cattle populations.These genome-wide variants serve as candidates for breed improvement and support conservation efforts for endangered cattle breeds against future climate changes.Therefore,this review plans to collate comprehensive information on the identified selection footprints to support genomic studies in African cattle to confirm the validity of the results and provide a framework for further genetic association and QTL fine mapping studies.
文摘The study was conducted to asses handling, processing, utilization, marketing as well as other socio-economic benefits of traditional cheese varieties making and to identify major constraints of traditional cheese varieties production in Awabal, Gozamin, Machakel and Dejen districts of Eastern Gojjam. A cross-sectional survey was conducted to collect data. A total of 240 smallholder dairy producers (60 households from each district) were individually interviewed using a semi-structured questionnaire. The major traditional cheese varieties produced in the study areas include Ayib, Metata, Hazo and Zureshekefign. Among these, three of them (Ayib, Metata and Hazo), are dominant. Respondents indicated that Metata cheese variety has high nutritional values and cure seven types of disease namely malaria and yellow fever, depressed appetite;Ejeseb (stomach upset), metabolic disorder (bloating), gastro-intestinal parasites, prevent nausea and digestion upset. The respondents reported twenty kinds of spices blended at one time during Metata cheese preparation and five kinds of spices commonly added into Ayib during Ayib making. Various types of herbs and plant species are used for smoking and washing of utensils during production of traditional cheese varieties in the study areas. The major marketing constraints of traditional cheese varieties were absence of sufficient buyer in the market, traditional taboos to sell cheese varieties in an open market, seasonal demand and supply, distance to the market, transportation access and quality. The major socio-economic benefits of traditional cheese varieties were to fulfill household necessities, sources of additional income, to purchase dairy inputs and for children school fee. Further scientific study needs to be conducted in order to confirm nutritional and medicinal properties of Metata cheese variety. Therefore the bio-active components of these spices and their effect on shelf life, nutritional value, medicinal uses and other attributes on traditional cheese varieties needed further investigation.
基金funded by the National Natural Science Foundation of China(31902180)the Top Talents Award Plan of Yangzhou University(2020)the Cyanine Project of Yangzhou University(2020)。
文摘Previous studies have revealed that dietary N-carbamylglutamate(NCG)or L-arginine(Arg)improves small intestinal integrity and immune function in suckling Hu lambs that have experienced intrauterine growth retardation(IUGR).Whether these nutrients alter redox status and apoptosis in the colon of IUGR lambs is still unknown.This study,therefore,aimed at investigating whether dietary supplementation of Arg or NCG alters colonic redox status,apoptosis and endoplasmic reticulum(ER)stress and the underlying mechanism of these alterations in IUGR suckling Hu lambs.Forty-eight 7-d old Hu lambs,including 12 with normal birth weight(4.25±0.14 kg)and 36 with IUGR(3.01±0.12 kg),were assigned to 4 treatment groups(n=12 each;6 males and 6 females)for 3 weeks.The treatment groups were control(CON),IUGR,IUGR+Arg and IUGR+NCG.Relative to IUGR lambs,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)content,as well as proliferation index,were higher(P<0.05)whereas reactive oxygen species(ROS),malondialdehyde(MDA)levels and apoptotic cell numbers were lower(P<0.05)in colonic tissue for both IUGR+Arg and NCG lambs.Both m RNA and protein levels of C/EBP homologous protein 10(CHOP10),B-cell lymphoma/leukaemia 2(Bcl-2)-associated X protein(Bax),apoptosis antigen 1(Fas),activating transcription factor 6(ATF6),caspase 3,and glucose-regulated protein 78(GRP78)were lower(P<0.05)while glutathione peroxidase 1(GPx1),Bcl-2 and catalase(CAT)levels were higher(P<0.05)in colonic tissue for IUGR+Arg and IUGR+NCG lambs compared with IUGR lambs.Based on our results,dietary NCG or Arg supplementation can improve colonic redox status and suppress apoptosis via death receptor-dependent,mitochondrial and ER stress pathways in IUGR suckling lambs.
基金The research was supported by the fund for the Top Talents Award Plan of Yangzhou University(2020)the Cyanine Project of Yangzhou University(2020).
文摘This study aimed to investigate the effects of dietary supplementation of underfed Hu ewes from d 35 to110 of gestation with either rumen-protected L-arginine(RP-Arg)or N-carbamylglutamate(NCG)on placental amino acid(AA)transport,angiogenic gene expression,and steroid anabolism.On d 35 of gestation,32 Hu ewes carrying twin fetuses were randomly divided into four treatment groups,each consisting of eight ewes,and were fed the following diets:A diet providing 100%of NRC’s nutrient requirements for pregnant ewes(CON);A diet providing 50%of NRC’s nutrient requirements for pregnant ewes(RES);RES diet plus 5 g/d NCG(RES+NCG);or RES diet plus 20 g/d RP-Arg(RES+ARG).On the d 110 of pregnancy,blood samples were taken from the mother,and samples were collected from type A cotyledons(COT;the fetal portions of the placenta).The levels of 17β-estradiol and progesterone in the maternal serum and both the capillary area density(CAD)and capillary surface density(CSD)in type A COT were decreased in response to Arg or NCG supplementation when compared to the RES group.The concentrations of arginine,leucine,putrescine and spermidine in type A COT were higher(P<0.05)in the RES+ARG or RES+NCG group than in the RES group.The mRNA expression levels of inducible nitric oxide synthase(iNOS)and solute carrier family 15,member 1(SLC15A1)were increased(P<0.05)while those of progesterone receptor(PGR)and fibroblast growth factor 2(FGF2)were decreased in type A COT by supplementation with either NCG or RP-Arg compared to the RES group.The results suggest that providing underfed pregnant ewes from d 35 to 110 of gestation with a diet supplemented with NCG or RP-Arg improves placental AA transport,and reduces the expression of angiogenic growth factor genes and steroid anabolism,leading to better fetal development.
基金funded by the Ministry of Education of Malaysia under the Fundamental Research Grant Scheme(FRGS)(R/FRGS/A07.00/00387A/005/2013/00107)Niche Research Grant Scheme(NRGS)(R/NRGS/A0.700/00387A/006/2014/00152)partially supported by Chiang Mai University。
文摘The aquafeed industry continues to expand in response to the rapidly growing aquaculture sector.However,the identification of alternative protein sources in aquatic animal diets to replace conventional sources due to cost and sustainability issues remains a major challenge.Recently,insects have shown tremendous results as potential replacers of fishmeal in aquafeed.The present study aimed to review the utilization of insects in aquafeeds and their effects on aquatic animals’growth and feed utilization,immune response and disease resistance,and fish flesh quality and safety.While many insect species have been investigated in aquaculture,the black soldier fly(Hermetia illucens),and the mealworm(Tenebrio molitor)are the most studied and most promising insects to replace fishmeal in aquafeed.Generally,insect rearing conditions and biomass processing methods may affect the product’s nutritional composition,digestibility,shelf life and required insect inclusion level by aquatic animals.Also,insect-recommended inclusion levels for aquatic animals vary depending on the insect species used,biomass processing method,and test organism.Overall,while an appropriate inclusion level of insects in aquafeed provides several nutritional and health benefits to aquatic animals,more studies are needed to establish optimum requirements levels for different aquaculture species at different stages of development and under different culture systems.
