Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gast...Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gastric/liver/:550/460/480)and 800 controls were recruited in this case-control study.The meta-analysis was performed by pooling the data from previous related studies and the present study.Results The results of this study showed that in the Hubei Han Chinese population,the rs10754339gene was significantly associated with the risk of lung and gastric cancer but not liver cancer,and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer.The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339and breast cancer risk,as well as between rs12976445 and lung cancer risk.Conclusion The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population,which should be validated in future studies with larger sample sizes in other ethnic populations.展开更多
We applied adaptive dynamics to double slit interference phenomenon using particle model and obtained partial successful results in our previous report. The patterns qualitatively corresponded well with experiments. S...We applied adaptive dynamics to double slit interference phenomenon using particle model and obtained partial successful results in our previous report. The patterns qualitatively corresponded well with experiments. Several properties such as concave single slit pattern and large influence of slight displacement of the emission position were different from the experimental results. In this study we tried other slit conditions and obtained consistent patterns with experiments. We do not claim that the adaptive dynamics is the principle of quantum mechanics, but the present results support the probability of adaptive dynamics as the candidate of the basis of quantum mechanics. We discuss the advantages of the adaptive dynamical view for foundations of quantum mechanics.展开更多
AIM To identify potential anti-cancer constituents in natural extracts that inhibit cancer cell growth and migration.METHODS Our experiments used high dose thymoquinone(TQ) as an inhibitor to arrest Lo Vo(a human colo...AIM To identify potential anti-cancer constituents in natural extracts that inhibit cancer cell growth and migration.METHODS Our experiments used high dose thymoquinone(TQ) as an inhibitor to arrest Lo Vo(a human colon adenocarcinoma cell line) cancer cell growth, which was detected by cell proliferation assay and immunoblotting assay. Low dose TQ did not significantly reduce Lo Vo cancer cell growth. Cyclooxygenase 2(COX-2) is an enzyme that is involved in the conversion of arachidonic acid into prostaglandin E2(PGE2) in humans. PGE2 can promote COX-2 protein expression and tumor cell proliferation and was used as a control.RESULTS Our results showed that 20 μmol/L TQ significantly reduced human LoV o colon cancer cell proliferation. TQ treatment reduced the levels of p-PI3 K, p-Akt, p-GSK3β, and β-catenin and thereby inhibited the downstream COX-2 expression. Results also showed that the reduction in COX-2 expression resulted in a reduction in PGE2 levels and the suppression of EP2 and EP4 activation. Further analysis showed that TG treatment inhibited the nuclear translocation of β-catenin in LoV o cancer cells. The levels of the cofactors LEF-1 and TCF-4 were also decreased in the nucleus following TQ treatment in a dose-dependent manner. Treatment with low dose TQ inhibited the COX-2 expression at the transcriptional level and the regulation of COX-2 expression efficiently reduced LoV o cell migration. The results were further verified in vivo by confirming the effects of TQ and/or PGE2 using tumor xenografts in nude mice.CONCLUSION TQ inhibits LoV o cancer cell growth and migration, and this result highlights the therapeutic advantage of using TQ in combination therapy against colorectal cancer.展开更多
The establishment of pregnancy is a complex process that requires a well-coordinated interaction between the implanting conceptus and the maternal uterus. In pigs, the conceptus undergoes dramatic morphological and fu...The establishment of pregnancy is a complex process that requires a well-coordinated interaction between the implanting conceptus and the maternal uterus. In pigs, the conceptus undergoes dramatic morphological and functional changes at the time of implantation and introduces various factors, including estrogens and cytokines,interleukin-1β2(IL1 B2), interferon-γ(IFNG), and IFN-δ(IFND), into the uterine lumen. In response to ovarian steroid hormones and conceptus-derived factors, the uterine endometrium becomes receptive to the implanting conceptus by changing its expression of cell adhesion molecules, secretory activity, and immune response. Conceptus-derived estrogens act as a signal for maternal recognition of pregnancy by changing the direction of prostaglandin(PG) F2αfrom the uterine vasculature to the uterine lumen. Estrogens also induce the expression of many endometrial genes,including genes related to growth factors, the synthesis and transport of PGs, and immunity. IL1 B2, a pro-inflammatory cytokine, is produced by the elongating conceptus. The direct effect of IL1 B2 on endometrial function is not fully understood. IL1 B activates the expression of endometrial genes, including the genes involved in IL1 B signaling and PG synthesis and transport. In addition, estrogen or IL1 B stimulates endometrial expression of IFN signaling molecules,suggesting that estrogen and IL1 B act cooperatively in priming the endometrial function of conceptus-produced IFNG and IFND that, in turn, modulate endometrial immune response during early pregnancy. This review addresses information about maternal-conceptus interactions with respect to endometrial gene expression in response to conceptus-derived factors, focusing on the roles of estrogen and IL1 B during early pregnancy in pigs.展开更多
Systemic studies on the effects of mitogen-activated protein kinase (MAPK) signal transduction pathway on the growthand development of Setosphaeria turcica is helpful not only in understanding the molecular mechanism ...Systemic studies on the effects of mitogen-activated protein kinase (MAPK) signal transduction pathway on the growthand development of Setosphaeria turcica is helpful not only in understanding the molecular mechanism of pathogen-host interaction but also in the effective control of the diseases caused by S. turcica. U0126, the specific MEK inhibitor,is used to treat S. turcica before the observation of the conidial germination, appressorium production, and pathogenicityof the pathogen. There is no significant effect of U0126 on the colony morphology and mycelium growth of the pathogen.After treatment with U0126, the growth of mycelium and conidia are normal, but the conidial germination, appressoriumproduction, and pathogenicity of S. turcica on susceptible corn leaves are significantly inhibited. Under the definiteconcentration scope, an increase in U0126 concentration increases the inhibition degree of conidial germination andappressorium production, but the inhibition degree decreases with elongation of treatment time. The conidial germination,appressorium production, and pathogenicity of S. turcica on susceptible corn leaves are regulated by the MAPK pathwayinhibited by U0126.展开更多
BACKGROUND Radiologic adjacent segment degeneration(ASDeg)can occur after spinal surgery.Adjacent segment disease(ASDis)is defined as the development of new clinical symptoms corresponding to radiographic changes adja...BACKGROUND Radiologic adjacent segment degeneration(ASDeg)can occur after spinal surgery.Adjacent segment disease(ASDis)is defined as the development of new clinical symptoms corresponding to radiographic changes adjacent to the level of previous spinal surgery.Greater pre-existing ASDeg is generally considered to result in more severe ASDis;nonetheless,whether the ASDeg status before index surgery influences the postoperative risk of revision surgery due to ASDis warrants investigation.AIM To identify possible risk factors for ASDis and verify the concept that greater preexisting ASDeg leads to more severe ASDis.METHODS Data from 212 patients who underwent posterior decompression with Dynesys stabilization from January 2006 to June 2016 were retrospectively analyzed.Patients who underwent surgery for ASDis were categorized as group A(n=13),whereas those who did not were classified as group B(n=199).Survival analysis and Cox proportional hazards models were used to compare the modified Pfirrmann grade,University of California-Los Angeles grade,body mass index,number of Dynesys-instrumented levels,and age.RESULTS The mean time of reoperation was 7.22(1.65–11.84)years in group A,and the mean follow-up period was 6.09(0.10–12.76)years in group B.No significant difference in reoperation risk was observed:Modified Pfirrmann grade 3 vs 4(P=0.53)or 4 vs 5(P=0.46)for the upper adjacent disc,University of California-Los Angeles grade 2 vs 3 for the upper adjacent segment(P=0.66),age of<60 vs>60 years(P=0.9),body mass index<25 vs>25 kg/m2(P=0.3),and sex(P=0.8).CONCLUSION Greater preexisting upper ASDeg was not associated with a higher rate of reoperation for ASDis after Dynesys surgery.Being overweight tended to increase reoperation risk after Dynesys surgery for ASDis.展开更多
<span style="font-family:Verdana;">This study was used</span><span style="font-family:Verdana;"> to</span><span style="font-family:;" "=""><...<span style="font-family:Verdana;">This study was used</span><span style="font-family:Verdana;"> to</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> lift nets and trap net fishing at intertidal zone of lagoon and estuarine regions of southern Taiwan. From July 2013 to July 2018, a total 420 specimens of </span><i><span style="font-family:Verdana;">Pisodonophis</span></i><span style="font-family:Verdana;"> were analyzed, the average total body length (TL) and average body weight (W) of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> eels are 68.4 ± 11.9 cm and 140.4 ± 86.1 g (N = 194), respectively. Typically, </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">cancrivorus</span></i><span style="font-family:Verdana;"> eels are smaller (TL = 52.8 ± 9.2 cm) and lighter (W = 107.9 ± 73.7 g [N = 119]) than </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> eels. The natural logarithmic linear relationships between the TL and W of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">cancrivorus</span></i><span style="font-family:Verdana;"> are respectively expressed as ln(W)</span><sub><span style="font-family:Verdana;">b</span></sub><span style="font-family:Verdana;"> = 2.4405 ln(TL) <span style="white-space:nowrap;">−</span> 5.4448 (R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.796, N = 194) and ln(W)</span><sub><span style="font-family:Verdana;">c</span></sub><span style="font-family:Verdana;"> = 3.2168 ln(TL) <span style="white-space:nowrap;">−</span> 8.2027, (</span><i><span style="font-family:Verdana;">R</span></i><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.934, N= 119). </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">cancrivorus</span></i><span style="font-family:Verdana;"> typically preys on shrimp, crab, and fish;lab assessment revealed that the frequencies of occurrence of these foods in their guts were 49%, 21%, and 15%, respectively (N = 100). The main catch months of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> were during the rainy season, and that of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">cancrivorus</span></i><span style="font-family:Verdana;"> were the winter season in Taiwan. The average W values of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> from Jhuoshuei and Puzih estuaries were 343.2 ± 218.8 g (N = 91) and 140.4 ± 86.1 g (N = 194), respectively. Furthermore, the results of the study revealed that the Puzih estuary is overfished. </span><span style="font-family:Verdana;">We suggest that fishery management units should develop some protection measures for the conservation of aquatic ecosystems in estuarine regions. For example, </span><span style="font-family:Verdana;">the </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> weighing less than 113 g will be released back to the estuary.</span></span>展开更多
Cancer is a serious threat to public health and the economy worldwide.Statistical data from the World Health Organization(WHO)in 2018 have demonstrated that lung cancer is the most commonly diagnosed cancer(11.6%of th...Cancer is a serious threat to public health and the economy worldwide.Statistical data from the World Health Organization(WHO)in 2018 have demonstrated that lung cancer is the most commonly diagnosed cancer(11.6%of the total cases)and the leading cause of cancer death(18.4%of the total cancer deaths),closely followed bygastric cancer(5.7%and 8.2%),and liver cancer(4.7%and 8.2%).展开更多
[Objective] This study aimed to clone and analyze the gene sequence encoding carbonic anhydrase-related protein 10-like (CARP X) from Apis mellifera. [Method] The cDNA sequence of CARPⅩ gene was cloned through RT-PCR...[Objective] This study aimed to clone and analyze the gene sequence encoding carbonic anhydrase-related protein 10-like (CARP X) from Apis mellifera. [Method] The cDNA sequence of CARPⅩ gene was cloned through RT-PCR, and then analyzed with bioinformatic method. [Result] The full-length cDNA sequence of CARPⅩ was 972 bp long and encoded 324 amino acid residues, including a signal peptide and two transmembrane domains. The predicted molecular mass was 37.1 kDa and the predicted isoelectric point was 7.458. The CARP X from A. mellifera shared close relationship with proteins from Apis florae, Bombus impatiens, Bombus terrestris, Nasonia vitripennis and Acyrthosiphon pisum. The insect CARP X family may include two subfamilies. [Conclusion] The results provide basis for studying CARPs family.展开更多
A promising bacterial strain for the biodegradation of Microcystins(MCs)was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3 mg·L -1 ...A promising bacterial strain for the biodegradation of Microcystins(MCs)was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3 mg·L -1 was completely degraded by USTB-05 within 36 h,which was a relatively high biodegradation rate of MC-RR.With the cell-free extract(CE)of Sphingopyxis sp.USTB-05,MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain,so that initial MC-RR of 42.3 mg·L -1 was completely biodegraded within 10 h.During the bio-reaction of MC-RR catalyzed by CE,two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm,indicating that the group of Adda in all products of MC-RR remained intact.展开更多
AIM:To compare the clinical outcome of single-incision laparoscopic cholecystectomy(SILC)and three-incision laparoscopic cholecystectomy(3ILC)for acute cholecystitis.METHODS:From July 2009 to September 2012,136patient...AIM:To compare the clinical outcome of single-incision laparoscopic cholecystectomy(SILC)and three-incision laparoscopic cholecystectomy(3ILC)for acute cholecystitis.METHODS:From July 2009 to September 2012,136patients underwent SILC or 3ILC for acute cholecystitis at a tertiary referral hospital.One experienced surgeon performed every procedure using 5 or 10 mm 30-degree laparoscopes,straight instruments,and conventional ports.Five patients with perforated gallbladder and diffuse peritonitis and 23 patients with mild acute cholecystitis were excluded.The remaining 108 patients were divided into complicated and uncomplicated groups according to pathologic findings.Patient demography,clinical data,operative results and complications were recorded and analyzed.RESULTS:Fifty patients with gangrenous cholecystitis,gallbladder empyema,or hydrops were classified as the complicated group,and 58 patients with acute cholecystitis were classified as the uncomplicated group.Twenty-three(46.0%)of the patients in the complicated group(n=50)and 39(67.2%)of the patients in the uncomplicated group(n=58)underwent SILC;all others underwent 3ILC.The postoperative length of hospital stay(PLOS)was significantly shorter in the SILC subgroups than the 3ILC subgroups(3.5±1.1 d vs 4.6±1.3 d,P<0.01 in the complicated group;2.9±1.1 d vs 3.7±1.4 d,P<0.05 in the uncomplicated group).The maximum body temperature recorded at day 1 and at day 2 following the procedure was lower in the SILC subgroups,but the difference reached statistical significance only in the uncomplicated group(37.41±0.56℃vs 37.80±0.72℃,P<0.05 on postoperative day 1;37.10±0.43℃vs 37.57±0.54℃,P<0.01 on postoperative day 2).The operative time,estimated blood loss,postoperative narcotic use,total length of hospital stay,conversion rates,and complication rates were similar in both SILC and 3ILC subgroups.The complicated group had longer operative time(122.2±35.0 min vs 106.6±43.6 min,P<0.05),longer PLOS(4.1±1.3 d vs 3.2±1.2 d,P<0.001),and higher conversion rates(36.0%vs 19.0%,P<0.05)compared with the uncomplicated group.CONCLUSION:SILC is safe and efficacious for patients with acute cholecystitis.The main benefit is a faster recovery than that achieved with 3ILC.展开更多
A strain,USTB-05,isolated from Lake Dianchi,China,degraded the cyanobacterial toxin microcystin-RR(MC-RR) at the rate of 16.7 mg/L per day.Analysis of 16S rDNA sequence showed that the strain was Sphingopyxis sp.Enzym...A strain,USTB-05,isolated from Lake Dianchi,China,degraded the cyanobacterial toxin microcystin-RR(MC-RR) at the rate of 16.7 mg/L per day.Analysis of 16S rDNA sequence showed that the strain was Sphingopyxis sp.Enzymatic degradation pathways for MC-RR by Sphingopyxis sp.USTB-05 were identified.Adda-Arg peptide bond of MC-RR was cleaved and then a hydrogen and a hydroxyl were combined onto the NH 2 group of Adda and the carboxyl group of arginine to form a linear molecule as intermediate product within the first few hours.Then,through dehydration reaction,two hydrogen of amino group on arginine reacted with adjacent hydroxyl on carbon to form a linear MC-RR with two small peptide rings as the final product after 24 hr.These biodegradation pathways were different from those reported for other strains,implying that MC-RR may undergo different transformations and different products were formed due to various bacteria in natural lakes and reservoirs.展开更多
AIM To elucidate the prevalence and risk of mortality of nonalcoholic liver cirrhosis(LC) patients with coronary artery disease(CAD).METHODS The study cohort included newly diagnosed nonalcoholic LC patients age ≥ 40...AIM To elucidate the prevalence and risk of mortality of nonalcoholic liver cirrhosis(LC) patients with coronary artery disease(CAD).METHODS The study cohort included newly diagnosed nonalcoholic LC patients age ≥ 40 years old without a diagnosis of CAD from 2006 until 2011 from a longitudinal health insurance database. The mean follow-up period for the study cohort was 1152 ± 633 d. The control cohort was matched by sex, age, residence, and index date. Hazard ratios(HRs) were calculated using the Cox proportional hazard model and the Kaplan-Meier method. RESULTS After exclusion, a total of 3409 newly diagnosed nonalcoholic cirrhotic patients were identified from one million samples from the health insurance database. We found that CAD(5.1% vs 17.4%) and hyperlipidemia(20.6% vs 24.1%) were less prevalent in nonalcoholic LC patients than in normal subjects(all P < 0.001), whereas other comorbidities exhibited an increased prevalence. Among the comorbidities, chronic kidney disease exhibited the highest risk for mortality(adjusted HR(AHR) = 1.76; 95%CI: 1.55-2.00, P < 0.001). Ascites or peritonitis exhibited the highest risk of mortality among nonalcoholic cirrhotic patients(AHR = 2.34; 95%CI: 2.06-2.65, P < 0.001). Finally, a total of 170 patients developed CAD after a diagnosis of nonalcoholic LC. The AHR of CAD in nonalcoholic LC patients was 0.56(95%CI: 0.43-0.74, P < 0.001). The six-year survival rates for nonalcoholic LC patients with and without CAD were 52% and 50%, respectively(P = 0.012). CONCLUSION We conclude that CAD was less prevalent and associated with a reduced risk of mortality in nonalcoholic cirrhotic patients.展开更多
Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplicat...Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplication event.It is unclear whether the duplicated homologs have conserved or diversi fied functions.Hydrogen sulfide(H_(2)S)is a plant gasotransmitter that plays important physiological roles in growth,development,and responses to environmental stresses.The modification of cysteines through S-sulfhydration is an important mechanism of H_(2)S,which regulates protein functions.H?S promotes flowering in Arabidopsis and heading Chinese cabbage.Here we investigated the molecular mechanisms of H_(2)S used to promote flowering in the latter.Four,five,and four BraFLC,BraSOC I,and BraFT homologs were identi fi ed in heading Chinese cabbage.Different BraFLC proteins were bound to different CArG boxes in the promoter regions of the BraSOC I and BraFT homologs,producing different binding patterns.Thus,there may be functionally diverse BraFLC homologs in heading Chinese cabbage.Exogenous H_(2)S at 100μmol L^(-1) significantly promoted flowering by compensating for insuf fi cient vernalization.BraFLC 1 and BraFLC_(3) underwent S-sulfhydration by H_(2)S,after which their abilities to bind most BraSOC I or BraFT promoter probes weakened or even disappeared.These changes in binding ability were consistent with the expression pattern of the BraFT and BraSOC I homologs in seedlings treated with H_(2)S.These results indicated that H_(2)S signaling regulates flowering time.In summary,H_(2)S signaling promoted plant flowering by weakening or eliminating the binding abilities of BraFLCs to downstream promoters through S-sulfhydration.展开更多
AIM: To investigate the effects of 17β-estradiol via estrogen receptors(ER) or direct administration of ER agonists on human colorectal cancer. METHODS: Lo Vo cells were established from the Bioresource Collection an...AIM: To investigate the effects of 17β-estradiol via estrogen receptors(ER) or direct administration of ER agonists on human colorectal cancer. METHODS: Lo Vo cells were established from the Bioresource Collection and Research Center and cultured in phenol red-free DMEM(Sigma, United States). To investigate the effects of E2 and/or ER selective agonists on cellular proliferation, Lo Vo colorectal cells were treated with E2 or ER-selective agonists for 24 h and 48 h and subjected to the MTT(Sigma) assay to find the concentration. And investigate the effects of E2 and/or ER selective agonists on cell used western immunoblotting to find out the diversification of signaling pathways. In order to observe motility and migration the wound healing assay and a transwell chamber(Neuro Probe) plate were tased. For a quantitative measure, we counted the number of migrating cells to the wound area post-wounding for 24 h. We further examined the cellular migration-regulating factors urokinase-type plasminogen activator(u-PA), tissue-type plasminogen activator(t-PA) and matrix metalloproteinase(MMP)-9 in human Lo Vo cells so gelatin zymography that we used and gelatinolytic activity was visualized by Coomassie blue staining. And these results are presented as means ± SE, and statistical comparisons were made using Student's t-test.RESULTS: The structure was first compared with E2 and ER agonists. We then treated the Lo Vo cells with E2 and ER agonists(10-8 mol/L) for 24 h and 48 h and subsequently measured the cell viability using MTT assay. Our results showed that treatment with 17β-estradiol and/or ER agonists in human Lo Vo colorectal cancer cells activated p53 and then up-regulated p21 and p27 protein levels, subsequently inhibiting the downstream target gene, cyclin D1, which regulates cell proliferation. Taken together, our findings demonstrate the anti-tumorigenesis effects of 17β-estradiol and/or ER agonists and suggest that these compounds may prove to be a potential alternative therapy in the treatment of human colorectal cancer. These results demonstrate that 17β-estradiol and/or ER agonists downregulate migration-related proteins through the p53 signaling pathway in human Lo Vo colorectal cancer cells. These findings suggest that p53 plays a critical role in the 17β-estradiol and/or ER agonist-mediated protective activity against colorectal cancer progression. In addition, 17β-estradiol and/or ER agonists dramatically inhibited cell migration and reduced the expression of u-PA, t-PA and MMP-9 as well as MMP-2/9 activity in Lo Vo cells, which regulate cell metastasis. Moreover, we observed that pretreatment with a p53 inhibitor significantly blocked the anti-migration effects of E2 and/or ER agonists on Lo Vo cells. That E2 and/or ER agonists may impair Lo Vo cell migration by modulating migration-related factors via the p53 tumor suppressor gene.CONCLUSION: Direct ER treatment may prove to be an attractive alternative therapy in the treatment of human colorectal tumors in the future.展开更多
BACKGROUND Cholangiocarcinoma is a highly lethal disease that had been underestimated in the past two decades. Many risk factors are well documented for in cholangiocarcinoma, but the impacts of advanced biliary inter...BACKGROUND Cholangiocarcinoma is a highly lethal disease that had been underestimated in the past two decades. Many risk factors are well documented for in cholangiocarcinoma, but the impacts of advanced biliary interventions, like endoscopic sphincterotomy(ES), endoscopic papillary balloon dilatation(EPBD),and cholecystectomy, are inconsistent in the previous literature.AIM To clarify the risks of cholangiocarcinoma after ES/EPBD, cholecystectomy or no intervention for cholelithiasis using the National Health Insurance Research Database(NHIRD).METHODS From data of NHIRD 2004-2011 in Taiwan, we selected 7938 cholelithiasis cases as well as 23814 control group cases(matched by sex and age in a 1:3 ratio). We compared the previous risk factors of cholangiocarcinoma and cholangiocarcinoma rate in the cholelithiasis and control groups. The incidences of total and subsequent cholangiocarcinoma were calculated in ES/EPBD patients, cholecystectomy patients, cholelithiasis patients without intervention,and groups from the normal population.RESULTS In total, 537 cases underwent ES/EPBD, 1743 cases underwent cholecystectomy,and 5658 cholelithiasis cases had no intervention. Eleven(2.05%), 37(0.65%), and7(0.40%) subsequent cholangiocarcinoma cases were diagnosed in the ES/EPBD,no intervention, and cholecystectomy groups, respectively, and the odds ratio for subsequent cholangiocarcinoma was 3.13 in the ES/EPBD group and 0.61 in the cholecystectomy group when compared with the no intervention group.CONCLUSION In conclusion, symptomatic cholelithiasis patients who undergo cholecystectomy can reduce the incidence of subsequent cholangiocarcinoma, while cholelithiasis patients who undergo ES/EPBD are at a great risk of subsequent cholangiocarcinoma according to our findings.展开更多
基金supported by the Fundamental Research Funds for the Central Universities (WUT:2020IB029)。
文摘Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gastric/liver/:550/460/480)and 800 controls were recruited in this case-control study.The meta-analysis was performed by pooling the data from previous related studies and the present study.Results The results of this study showed that in the Hubei Han Chinese population,the rs10754339gene was significantly associated with the risk of lung and gastric cancer but not liver cancer,and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer.The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339and breast cancer risk,as well as between rs12976445 and lung cancer risk.Conclusion The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population,which should be validated in future studies with larger sample sizes in other ethnic populations.
文摘We applied adaptive dynamics to double slit interference phenomenon using particle model and obtained partial successful results in our previous report. The patterns qualitatively corresponded well with experiments. Several properties such as concave single slit pattern and large influence of slight displacement of the emission position were different from the experimental results. In this study we tried other slit conditions and obtained consistent patterns with experiments. We do not claim that the adaptive dynamics is the principle of quantum mechanics, but the present results support the probability of adaptive dynamics as the candidate of the basis of quantum mechanics. We discuss the advantages of the adaptive dynamical view for foundations of quantum mechanics.
基金Supported by (in part) the Taiwan Ministry of Health and Welfare Clinical Trial and Research Center of Excellence,No.MOHW105-TDU-B-212-133019
文摘AIM To identify potential anti-cancer constituents in natural extracts that inhibit cancer cell growth and migration.METHODS Our experiments used high dose thymoquinone(TQ) as an inhibitor to arrest Lo Vo(a human colon adenocarcinoma cell line) cancer cell growth, which was detected by cell proliferation assay and immunoblotting assay. Low dose TQ did not significantly reduce Lo Vo cancer cell growth. Cyclooxygenase 2(COX-2) is an enzyme that is involved in the conversion of arachidonic acid into prostaglandin E2(PGE2) in humans. PGE2 can promote COX-2 protein expression and tumor cell proliferation and was used as a control.RESULTS Our results showed that 20 μmol/L TQ significantly reduced human LoV o colon cancer cell proliferation. TQ treatment reduced the levels of p-PI3 K, p-Akt, p-GSK3β, and β-catenin and thereby inhibited the downstream COX-2 expression. Results also showed that the reduction in COX-2 expression resulted in a reduction in PGE2 levels and the suppression of EP2 and EP4 activation. Further analysis showed that TG treatment inhibited the nuclear translocation of β-catenin in LoV o cancer cells. The levels of the cofactors LEF-1 and TCF-4 were also decreased in the nucleus following TQ treatment in a dose-dependent manner. Treatment with low dose TQ inhibited the COX-2 expression at the transcriptional level and the regulation of COX-2 expression efficiently reduced LoV o cell migration. The results were further verified in vivo by confirming the effects of TQ and/or PGE2 using tumor xenografts in nude mice.CONCLUSION TQ inhibits LoV o cancer cell growth and migration, and this result highlights the therapeutic advantage of using TQ in combination therapy against colorectal cancer.
基金Support for the work from the authors’laboratory described in this review paper has been provided by the Bio Green 21 Program(20050603050120070301034040+8 种基金 20080401034003 PJ007997 PJ009610 PJ01110301PJ01119103)the Rural Development Administrationa National Research Foundation grant funded by the Korean Government(KRF-2005-003-F00017,KRF-2007-521-F00030,NRF-2010-0012304,NRF-2010-10012304 NRF-2012R1A2A2A01047079 NRF-2015R1D1A1A01058356,Republic of Korea
文摘The establishment of pregnancy is a complex process that requires a well-coordinated interaction between the implanting conceptus and the maternal uterus. In pigs, the conceptus undergoes dramatic morphological and functional changes at the time of implantation and introduces various factors, including estrogens and cytokines,interleukin-1β2(IL1 B2), interferon-γ(IFNG), and IFN-δ(IFND), into the uterine lumen. In response to ovarian steroid hormones and conceptus-derived factors, the uterine endometrium becomes receptive to the implanting conceptus by changing its expression of cell adhesion molecules, secretory activity, and immune response. Conceptus-derived estrogens act as a signal for maternal recognition of pregnancy by changing the direction of prostaglandin(PG) F2αfrom the uterine vasculature to the uterine lumen. Estrogens also induce the expression of many endometrial genes,including genes related to growth factors, the synthesis and transport of PGs, and immunity. IL1 B2, a pro-inflammatory cytokine, is produced by the elongating conceptus. The direct effect of IL1 B2 on endometrial function is not fully understood. IL1 B activates the expression of endometrial genes, including the genes involved in IL1 B signaling and PG synthesis and transport. In addition, estrogen or IL1 B stimulates endometrial expression of IFN signaling molecules,suggesting that estrogen and IL1 B act cooperatively in priming the endometrial function of conceptus-produced IFNG and IFND that, in turn, modulate endometrial immune response during early pregnancy. This review addresses information about maternal-conceptus interactions with respect to endometrial gene expression in response to conceptus-derived factors, focusing on the roles of estrogen and IL1 B during early pregnancy in pigs.
基金supported by the National Natural Science Foundation of China(30471126)Doctoral Foundation Project of Hebei Province,China(05547007D-2).
文摘Systemic studies on the effects of mitogen-activated protein kinase (MAPK) signal transduction pathway on the growthand development of Setosphaeria turcica is helpful not only in understanding the molecular mechanism of pathogen-host interaction but also in the effective control of the diseases caused by S. turcica. U0126, the specific MEK inhibitor,is used to treat S. turcica before the observation of the conidial germination, appressorium production, and pathogenicityof the pathogen. There is no significant effect of U0126 on the colony morphology and mycelium growth of the pathogen.After treatment with U0126, the growth of mycelium and conidia are normal, but the conidial germination, appressoriumproduction, and pathogenicity of S. turcica on susceptible corn leaves are significantly inhibited. Under the definiteconcentration scope, an increase in U0126 concentration increases the inhibition degree of conidial germination andappressorium production, but the inhibition degree decreases with elongation of treatment time. The conidial germination,appressorium production, and pathogenicity of S. turcica on susceptible corn leaves are regulated by the MAPK pathwayinhibited by U0126.
基金The study was approved by our institutional review board,Research Ethics Committee China Medical University and Hospital,Taichung,Taiwan(Protocol No.:CMUH108-REC2-133).
文摘BACKGROUND Radiologic adjacent segment degeneration(ASDeg)can occur after spinal surgery.Adjacent segment disease(ASDis)is defined as the development of new clinical symptoms corresponding to radiographic changes adjacent to the level of previous spinal surgery.Greater pre-existing ASDeg is generally considered to result in more severe ASDis;nonetheless,whether the ASDeg status before index surgery influences the postoperative risk of revision surgery due to ASDis warrants investigation.AIM To identify possible risk factors for ASDis and verify the concept that greater preexisting ASDeg leads to more severe ASDis.METHODS Data from 212 patients who underwent posterior decompression with Dynesys stabilization from January 2006 to June 2016 were retrospectively analyzed.Patients who underwent surgery for ASDis were categorized as group A(n=13),whereas those who did not were classified as group B(n=199).Survival analysis and Cox proportional hazards models were used to compare the modified Pfirrmann grade,University of California-Los Angeles grade,body mass index,number of Dynesys-instrumented levels,and age.RESULTS The mean time of reoperation was 7.22(1.65–11.84)years in group A,and the mean follow-up period was 6.09(0.10–12.76)years in group B.No significant difference in reoperation risk was observed:Modified Pfirrmann grade 3 vs 4(P=0.53)or 4 vs 5(P=0.46)for the upper adjacent disc,University of California-Los Angeles grade 2 vs 3 for the upper adjacent segment(P=0.66),age of<60 vs>60 years(P=0.9),body mass index<25 vs>25 kg/m2(P=0.3),and sex(P=0.8).CONCLUSION Greater preexisting upper ASDeg was not associated with a higher rate of reoperation for ASDis after Dynesys surgery.Being overweight tended to increase reoperation risk after Dynesys surgery for ASDis.
文摘<span style="font-family:Verdana;">This study was used</span><span style="font-family:Verdana;"> to</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> lift nets and trap net fishing at intertidal zone of lagoon and estuarine regions of southern Taiwan. From July 2013 to July 2018, a total 420 specimens of </span><i><span style="font-family:Verdana;">Pisodonophis</span></i><span style="font-family:Verdana;"> were analyzed, the average total body length (TL) and average body weight (W) of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> eels are 68.4 ± 11.9 cm and 140.4 ± 86.1 g (N = 194), respectively. Typically, </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">cancrivorus</span></i><span style="font-family:Verdana;"> eels are smaller (TL = 52.8 ± 9.2 cm) and lighter (W = 107.9 ± 73.7 g [N = 119]) than </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> eels. The natural logarithmic linear relationships between the TL and W of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">cancrivorus</span></i><span style="font-family:Verdana;"> are respectively expressed as ln(W)</span><sub><span style="font-family:Verdana;">b</span></sub><span style="font-family:Verdana;"> = 2.4405 ln(TL) <span style="white-space:nowrap;">−</span> 5.4448 (R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.796, N = 194) and ln(W)</span><sub><span style="font-family:Verdana;">c</span></sub><span style="font-family:Verdana;"> = 3.2168 ln(TL) <span style="white-space:nowrap;">−</span> 8.2027, (</span><i><span style="font-family:Verdana;">R</span></i><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.934, N= 119). </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">cancrivorus</span></i><span style="font-family:Verdana;"> typically preys on shrimp, crab, and fish;lab assessment revealed that the frequencies of occurrence of these foods in their guts were 49%, 21%, and 15%, respectively (N = 100). The main catch months of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> were during the rainy season, and that of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">cancrivorus</span></i><span style="font-family:Verdana;"> were the winter season in Taiwan. The average W values of </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> from Jhuoshuei and Puzih estuaries were 343.2 ± 218.8 g (N = 91) and 140.4 ± 86.1 g (N = 194), respectively. Furthermore, the results of the study revealed that the Puzih estuary is overfished. </span><span style="font-family:Verdana;">We suggest that fishery management units should develop some protection measures for the conservation of aquatic ecosystems in estuarine regions. For example, </span><span style="font-family:Verdana;">the </span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">boro</span></i><span style="font-family:Verdana;"> weighing less than 113 g will be released back to the estuary.</span></span>
基金supported by grants from the Natural Science Foundation of Hubei Province[2019CFB756]the Fundamental Research Funds for the Central Universities[WUT:2020IB029]。
文摘Cancer is a serious threat to public health and the economy worldwide.Statistical data from the World Health Organization(WHO)in 2018 have demonstrated that lung cancer is the most commonly diagnosed cancer(11.6%of the total cases)and the leading cause of cancer death(18.4%of the total cancer deaths),closely followed bygastric cancer(5.7%and 8.2%),and liver cancer(4.7%and 8.2%).
基金Supported by the Science and Technology Project of Education Commission of Shaanxi Province (11JK0618)
文摘[Objective] This study aimed to clone and analyze the gene sequence encoding carbonic anhydrase-related protein 10-like (CARP X) from Apis mellifera. [Method] The cDNA sequence of CARPⅩ gene was cloned through RT-PCR, and then analyzed with bioinformatic method. [Result] The full-length cDNA sequence of CARPⅩ was 972 bp long and encoded 324 amino acid residues, including a signal peptide and two transmembrane domains. The predicted molecular mass was 37.1 kDa and the predicted isoelectric point was 7.458. The CARP X from A. mellifera shared close relationship with proteins from Apis florae, Bombus impatiens, Bombus terrestris, Nasonia vitripennis and Acyrthosiphon pisum. The insect CARP X family may include two subfamilies. [Conclusion] The results provide basis for studying CARPs family.
基金Supported by the State Key Development Program for Basic Research of China（2008CB418105） the National Natural Science Foundation of China（203777008 20621703）+1 种基金 the State Key Joint Laboratory of Environment Simulation and Pollution Control（09K08ESPCT） the Educational Committee of Beijing
文摘A promising bacterial strain for the biodegradation of Microcystins(MCs)was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3 mg·L -1 was completely degraded by USTB-05 within 36 h,which was a relatively high biodegradation rate of MC-RR.With the cell-free extract(CE)of Sphingopyxis sp.USTB-05,MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain,so that initial MC-RR of 42.3 mg·L -1 was completely biodegraded within 10 h.During the bio-reaction of MC-RR catalyzed by CE,two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm,indicating that the group of Adda in all products of MC-RR remained intact.
基金Project supported by the National Natural Science Foundation of China (No.20377047,20507002)Innovation Foundation in University of Science and Technology Beijing.
文摘AIM:To compare the clinical outcome of single-incision laparoscopic cholecystectomy(SILC)and three-incision laparoscopic cholecystectomy(3ILC)for acute cholecystitis.METHODS:From July 2009 to September 2012,136patients underwent SILC or 3ILC for acute cholecystitis at a tertiary referral hospital.One experienced surgeon performed every procedure using 5 or 10 mm 30-degree laparoscopes,straight instruments,and conventional ports.Five patients with perforated gallbladder and diffuse peritonitis and 23 patients with mild acute cholecystitis were excluded.The remaining 108 patients were divided into complicated and uncomplicated groups according to pathologic findings.Patient demography,clinical data,operative results and complications were recorded and analyzed.RESULTS:Fifty patients with gangrenous cholecystitis,gallbladder empyema,or hydrops were classified as the complicated group,and 58 patients with acute cholecystitis were classified as the uncomplicated group.Twenty-three(46.0%)of the patients in the complicated group(n=50)and 39(67.2%)of the patients in the uncomplicated group(n=58)underwent SILC;all others underwent 3ILC.The postoperative length of hospital stay(PLOS)was significantly shorter in the SILC subgroups than the 3ILC subgroups(3.5±1.1 d vs 4.6±1.3 d,P<0.01 in the complicated group;2.9±1.1 d vs 3.7±1.4 d,P<0.05 in the uncomplicated group).The maximum body temperature recorded at day 1 and at day 2 following the procedure was lower in the SILC subgroups,but the difference reached statistical significance only in the uncomplicated group(37.41±0.56℃vs 37.80±0.72℃,P<0.05 on postoperative day 1;37.10±0.43℃vs 37.57±0.54℃,P<0.01 on postoperative day 2).The operative time,estimated blood loss,postoperative narcotic use,total length of hospital stay,conversion rates,and complication rates were similar in both SILC and 3ILC subgroups.The complicated group had longer operative time(122.2±35.0 min vs 106.6±43.6 min,P<0.05),longer PLOS(4.1±1.3 d vs 3.2±1.2 d,P<0.001),and higher conversion rates(36.0%vs 19.0%,P<0.05)compared with the uncomplicated group.CONCLUSION:SILC is safe and efficacious for patients with acute cholecystitis.The main benefit is a faster recovery than that achieved with 3ILC.
基金supported by the Chinese National Key Project for Basic Research (No.2008CB418105)the National Natural Science Foundation of China(No.20621703,20477050)the Important-Direction-Project funded by the Chinese Academy of Sciences
文摘A strain,USTB-05,isolated from Lake Dianchi,China,degraded the cyanobacterial toxin microcystin-RR(MC-RR) at the rate of 16.7 mg/L per day.Analysis of 16S rDNA sequence showed that the strain was Sphingopyxis sp.Enzymatic degradation pathways for MC-RR by Sphingopyxis sp.USTB-05 were identified.Adda-Arg peptide bond of MC-RR was cleaved and then a hydrogen and a hydroxyl were combined onto the NH 2 group of Adda and the carboxyl group of arginine to form a linear molecule as intermediate product within the first few hours.Then,through dehydration reaction,two hydrogen of amino group on arginine reacted with adjacent hydroxyl on carbon to form a linear MC-RR with two small peptide rings as the final product after 24 hr.These biodegradation pathways were different from those reported for other strains,implying that MC-RR may undergo different transformations and different products were formed due to various bacteria in natural lakes and reservoirs.
基金This work is supported by the Agricultural Key Foundation of Shanghai, China （No. 2006-5-6）, the National Natural Science Foundation of China （No. 3091490）, the National Basic Research Program, China （No. 2009CB941704）, PhD Programs Foundation of Ministry of Education of China （No. 200802480026） and the Shanghai Leading Academic Discipline Project, China （No. B205）. We would like to extend our appreciation to Prof. Yi-Tao Zeng of the Shanghai Institute of Medical Genetics and Professor Lin He of the Bio-X Center of the Shanghai Jiao Tong University for their insightful comments.
基金Supported by Chung Shan Medical University Hospital,Taichung,Taiwan,No.CSH-2013-C-032
文摘AIM To elucidate the prevalence and risk of mortality of nonalcoholic liver cirrhosis(LC) patients with coronary artery disease(CAD).METHODS The study cohort included newly diagnosed nonalcoholic LC patients age ≥ 40 years old without a diagnosis of CAD from 2006 until 2011 from a longitudinal health insurance database. The mean follow-up period for the study cohort was 1152 ± 633 d. The control cohort was matched by sex, age, residence, and index date. Hazard ratios(HRs) were calculated using the Cox proportional hazard model and the Kaplan-Meier method. RESULTS After exclusion, a total of 3409 newly diagnosed nonalcoholic cirrhotic patients were identified from one million samples from the health insurance database. We found that CAD(5.1% vs 17.4%) and hyperlipidemia(20.6% vs 24.1%) were less prevalent in nonalcoholic LC patients than in normal subjects(all P < 0.001), whereas other comorbidities exhibited an increased prevalence. Among the comorbidities, chronic kidney disease exhibited the highest risk for mortality(adjusted HR(AHR) = 1.76; 95%CI: 1.55-2.00, P < 0.001). Ascites or peritonitis exhibited the highest risk of mortality among nonalcoholic cirrhotic patients(AHR = 2.34; 95%CI: 2.06-2.65, P < 0.001). Finally, a total of 170 patients developed CAD after a diagnosis of nonalcoholic LC. The AHR of CAD in nonalcoholic LC patients was 0.56(95%CI: 0.43-0.74, P < 0.001). The six-year survival rates for nonalcoholic LC patients with and without CAD were 52% and 50%, respectively(P = 0.012). CONCLUSION We conclude that CAD was less prevalent and associated with a reduced risk of mortality in nonalcoholic cirrhotic patients.
基金the National Natural Science Foundation of China(31972428 and 31672140)the Shanxi Province Natural Science Foundation(201801D121191 and 201801D121197).
文摘Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplication event.It is unclear whether the duplicated homologs have conserved or diversi fied functions.Hydrogen sulfide(H_(2)S)is a plant gasotransmitter that plays important physiological roles in growth,development,and responses to environmental stresses.The modification of cysteines through S-sulfhydration is an important mechanism of H_(2)S,which regulates protein functions.H?S promotes flowering in Arabidopsis and heading Chinese cabbage.Here we investigated the molecular mechanisms of H_(2)S used to promote flowering in the latter.Four,five,and four BraFLC,BraSOC I,and BraFT homologs were identi fi ed in heading Chinese cabbage.Different BraFLC proteins were bound to different CArG boxes in the promoter regions of the BraSOC I and BraFT homologs,producing different binding patterns.Thus,there may be functionally diverse BraFLC homologs in heading Chinese cabbage.Exogenous H_(2)S at 100μmol L^(-1) significantly promoted flowering by compensating for insuf fi cient vernalization.BraFLC 1 and BraFLC_(3) underwent S-sulfhydration by H_(2)S,after which their abilities to bind most BraSOC I or BraFT promoter probes weakened or even disappeared.These changes in binding ability were consistent with the expression pattern of the BraFT and BraSOC I homologs in seedlings treated with H_(2)S.These results indicated that H_(2)S signaling regulates flowering time.In summary,H_(2)S signaling promoted plant flowering by weakening or eliminating the binding abilities of BraFLCs to downstream promoters through S-sulfhydration.
基金Supported by Taiwan Department of Health Clinical Trial and Re-search Center of Excellence No.MOHW103-TDU-B-212-113002
文摘AIM: To investigate the effects of 17β-estradiol via estrogen receptors(ER) or direct administration of ER agonists on human colorectal cancer. METHODS: Lo Vo cells were established from the Bioresource Collection and Research Center and cultured in phenol red-free DMEM(Sigma, United States). To investigate the effects of E2 and/or ER selective agonists on cellular proliferation, Lo Vo colorectal cells were treated with E2 or ER-selective agonists for 24 h and 48 h and subjected to the MTT(Sigma) assay to find the concentration. And investigate the effects of E2 and/or ER selective agonists on cell used western immunoblotting to find out the diversification of signaling pathways. In order to observe motility and migration the wound healing assay and a transwell chamber(Neuro Probe) plate were tased. For a quantitative measure, we counted the number of migrating cells to the wound area post-wounding for 24 h. We further examined the cellular migration-regulating factors urokinase-type plasminogen activator(u-PA), tissue-type plasminogen activator(t-PA) and matrix metalloproteinase(MMP)-9 in human Lo Vo cells so gelatin zymography that we used and gelatinolytic activity was visualized by Coomassie blue staining. And these results are presented as means ± SE, and statistical comparisons were made using Student's t-test.RESULTS: The structure was first compared with E2 and ER agonists. We then treated the Lo Vo cells with E2 and ER agonists(10-8 mol/L) for 24 h and 48 h and subsequently measured the cell viability using MTT assay. Our results showed that treatment with 17β-estradiol and/or ER agonists in human Lo Vo colorectal cancer cells activated p53 and then up-regulated p21 and p27 protein levels, subsequently inhibiting the downstream target gene, cyclin D1, which regulates cell proliferation. Taken together, our findings demonstrate the anti-tumorigenesis effects of 17β-estradiol and/or ER agonists and suggest that these compounds may prove to be a potential alternative therapy in the treatment of human colorectal cancer. These results demonstrate that 17β-estradiol and/or ER agonists downregulate migration-related proteins through the p53 signaling pathway in human Lo Vo colorectal cancer cells. These findings suggest that p53 plays a critical role in the 17β-estradiol and/or ER agonist-mediated protective activity against colorectal cancer progression. In addition, 17β-estradiol and/or ER agonists dramatically inhibited cell migration and reduced the expression of u-PA, t-PA and MMP-9 as well as MMP-2/9 activity in Lo Vo cells, which regulate cell metastasis. Moreover, we observed that pretreatment with a p53 inhibitor significantly blocked the anti-migration effects of E2 and/or ER agonists on Lo Vo cells. That E2 and/or ER agonists may impair Lo Vo cell migration by modulating migration-related factors via the p53 tumor suppressor gene.CONCLUSION: Direct ER treatment may prove to be an attractive alternative therapy in the treatment of human colorectal tumors in the future.
基金Chung Shan Medical University Hospital research program,Taichung,Taiwan,No.CSH-2013-C-032
文摘BACKGROUND Cholangiocarcinoma is a highly lethal disease that had been underestimated in the past two decades. Many risk factors are well documented for in cholangiocarcinoma, but the impacts of advanced biliary interventions, like endoscopic sphincterotomy(ES), endoscopic papillary balloon dilatation(EPBD),and cholecystectomy, are inconsistent in the previous literature.AIM To clarify the risks of cholangiocarcinoma after ES/EPBD, cholecystectomy or no intervention for cholelithiasis using the National Health Insurance Research Database(NHIRD).METHODS From data of NHIRD 2004-2011 in Taiwan, we selected 7938 cholelithiasis cases as well as 23814 control group cases(matched by sex and age in a 1:3 ratio). We compared the previous risk factors of cholangiocarcinoma and cholangiocarcinoma rate in the cholelithiasis and control groups. The incidences of total and subsequent cholangiocarcinoma were calculated in ES/EPBD patients, cholecystectomy patients, cholelithiasis patients without intervention,and groups from the normal population.RESULTS In total, 537 cases underwent ES/EPBD, 1743 cases underwent cholecystectomy,and 5658 cholelithiasis cases had no intervention. Eleven(2.05%), 37(0.65%), and7(0.40%) subsequent cholangiocarcinoma cases were diagnosed in the ES/EPBD,no intervention, and cholecystectomy groups, respectively, and the odds ratio for subsequent cholangiocarcinoma was 3.13 in the ES/EPBD group and 0.61 in the cholecystectomy group when compared with the no intervention group.CONCLUSION In conclusion, symptomatic cholelithiasis patients who undergo cholecystectomy can reduce the incidence of subsequent cholangiocarcinoma, while cholelithiasis patients who undergo ES/EPBD are at a great risk of subsequent cholangiocarcinoma according to our findings.
