The number of people diagnosed with diabetes continues to increase,especially among younger populations.Apart from genetic predisposition and lifestyle,there is increasing scientific and public concern that environmen...The number of people diagnosed with diabetes continues to increase,especially among younger populations.Apart from genetic predisposition and lifestyle,there is increasing scientific and public concern that environmental agents may also contribute to diabetes.Food contamination by chemical substances that originate from packaging materials,or are the result of chemical reactions during food processing,is generally recognized as a worldwide problem with potential health hazards.Phthalates,bisphenol A(BPA)and acrylamide(AA)have been the focus of attention in recent years,due to the numerous adverse health effects associated with their exposure.This paper summarizes the available data about the association between phthalates,BPA and AA exposure and diabetes.Although their mechanism of action has not been fully clarified,in vitro,in vivo and epidemiological studies have made significant progress toward identifying the potential roles of phthalates,BPA and AA in diabetes development and progression.These chemicals interfere with multiple signaling pathways involved in glucose and lipid homeostasis and can aggravate the symptoms of diabetes.Especially concerning are the effects of exposure during early stages and the gestational period.Well-designed prospective studies are needed in order to better establish prevention strategies against the harmful effects of these food contaminants.展开更多
Objective:To investigate the total phenolics,flavonoids and tannins content and the in vitro antioxidant activities of methanolic extracts of six wild Mentha species which are Mentha aquatica,Mentha arvensis,Mentha pi...Objective:To investigate the total phenolics,flavonoids and tannins content and the in vitro antioxidant activities of methanolic extracts of six wild Mentha species which are Mentha aquatica,Mentha arvensis,Mentha piperita,Mentha pulegium,Mentha rotundifolia and Mentha villosa.Methods:The Folin–Ciocalteu method was used to determine the total phenols content while flavonoids were estimated according to the aluminum chloride colorimetric method.To evaluate tannins content,vanillin and HCl were added to methanolic extracts.The antioxidant potential was measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging,ferrous ion chelating and the inhibition of b-carotene bleaching assays.Results:The methanol extracts of Algerian mints were rich in phenolic compounds and exhibited powerful antioxidant activity ranging from 7.5 mg/mL to 44.66 mg/mL,which varied significantly among species.Mentha aquatica stood out with efficient antioxidant ability which was correlated to the high total phenolics content,followed by Mentha arvensis and Mentha piperita with very close values,comparing to Mentha pulegium,Mentha rotundifolia and Mentha villosa with lowest values.Conclusions:These results show that methanolic extracts of Mentha species from Algeria have a great potential of polyphenols which can be used as a natural food preservative and antioxidant source.展开更多
Objective:To examine the effect of Rumex crispus(R.crispus)and Rumex sanguineus(R.sanguineus)plant extracts against isolates of Acinetobacter baumannii(A.baumannii)from wounds,including multidrug-resistant strains.Met...Objective:To examine the effect of Rumex crispus(R.crispus)and Rumex sanguineus(R.sanguineus)plant extracts against isolates of Acinetobacter baumannii(A.baumannii)from wounds,including multidrug-resistant strains.Methods:Six prepared Rumex extracts were subjected to liquid chromatography-tandem mass spectrometry.Antimicrobial activity of extracts and pure compounds(catechin,quercetin,isoquercitrin,emodin,and gallic acid)was examined by a microtiter plate method,while for determination of compound binary combinations activity a checkerboard method was applied.Active fractions of extracts were detected by agar-overlay high-performance thinlayer chromatography-bioautography assay followed by liquid chromatography-diode array detection-mass spectrometry analysis.Results:A total of 28 compounds were detected in two extracts of R.crispus and 26 compounds in four different R.sanguineus extracts,with catechin as a dominant component.Anti-A.baumannii activity was confirmed for all six R.sanguineus and R.crispus extracts at the concentration range from 1 to 4 mg/mL.Neither examined single compounds nor their binary combinations exhibited an anti-A.baumannii activity(MIC>256μg/mL).The bioautography showed that fractions with the most prominent anti-A.baumannii activity tended to contain more polar compounds,predominantly flavonol(quercetin and kaempherol)glycosides;but also fractions containing flavanone(eriodictyol)glycosides and anthraquinone(emodin)glycosides;and less polar eriodictyol aglycone.Conclusions:The results justify and elucidate the traditional application of R.sanguineus and R.crispus extracts for wound healing,indicating the necessity for their further examination in combat against multidrug-resistant A.baumannii isolates from wounds.展开更多
Inter Simple Sequence Repeats(ISSR) markers were used to assess genetic diversity within and among populations of dwarf mountain pine(Pinus mugo Turra) growing in the Tatra National Park(UNESCO Biosphere Reserve) in S...Inter Simple Sequence Repeats(ISSR) markers were used to assess genetic diversity within and among populations of dwarf mountain pine(Pinus mugo Turra) growing in the Tatra National Park(UNESCO Biosphere Reserve) in Southern Poland(Central Europe). The analyzed population belongs to two different geobotanical sub-districts: the Western and High Tatras. The level of genetic diversity assessed in this study for P. mugo is generally comparable to that reported for the other pine species in the Pinaceae family assessed by ISSR markers, especially with respect to Nei's genetic diversity and the percentage of polymorphic bands. Bayesian analysis clustered the analyzed populations into two groups, corresponding to their geobotanical locations in the Tatras. Significant divergence between the two genetical clusters was supported by the results of Analysis of Molecular Variance(AMOVA). According to the Mantel test, there was no correlation between the genetic distance and the geographical distance. The present study confirms the existence of two genetically distinct clusters of P. mugo populations in the Tatra Mountains. The observed high population-genetic differentiation of P.mugo in the Tatras could be attributed to several genetic, environmental and historical factors occurring in this mountain area.展开更多
For the first time, the anatomical structure of the vegetative organs of the species J. hippolyti and J. narbuti was studied in the conditions of Kyzylkum (Uzbekistan, Province Bukhara) and revealed the diagnostic fea...For the first time, the anatomical structure of the vegetative organs of the species J. hippolyti and J. narbuti was studied in the conditions of Kyzylkum (Uzbekistan, Province Bukhara) and revealed the diagnostic features of these species. A different combination of xeromorphic and mesomorphic features was determined, which ensures adaptation to habitat conditions. In J. narbuti xeromorphic features predominate, which indicates a more adaptable and wide distribution in the natural habitat of this species. In J. hippolyti—mesomorphic signs that show the rarity of the species in their natural habitat, compared to J. narbuti.展开更多
Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (...Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His? TA102 strain. Inhibition values of 44.2% and 67.0% were detected for t-BHP and H2O2, respectively. A protective ability of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in plasmid DNA showed a significant inhibition of DNA damage.展开更多
BACKGROUND A major problem in the healing of bone defects is insufficient or absent blood supply within the defect.To overcome this challenging problem,a plethora of approaches within bone tissue engineering have been...BACKGROUND A major problem in the healing of bone defects is insufficient or absent blood supply within the defect.To overcome this challenging problem,a plethora of approaches within bone tissue engineering have been developed recently.Bearing in mind that the interplay of various diffusible factors released by endothelial cells(ECs)and osteoblasts(OBs)have a pivotal role in bone growth and regeneration and that adjacent ECs and OBs also communicate directly through gap junctions,we set the focus on the simultaneous application of these cell types together with platelet-rich plasma(PRP)as a growth factor reservoir within ectopic bone tissue engineering constructs.AIM To vascularize and examine osteogenesis in bone tissue engineering constructs enriched with PRP and adipose-derived stem cells(ASCs)induced into ECs and OBs.METHODS ASCs isolated from adipose tissue,induced in vitro into ECs,OBs or just expanded were used for implant construction as followed:BPEO,endothelial and osteogenic differentiated ASCs with PRP and bone mineral matrix;BPUI,uninduced ASCs with PRP and bone mineral matrix;BC(control),only bone mineral matrix.At 1,2,4 and 8 wk after subcutaneous implantation in mice,implants were extracted and endothelial-related and bone-related gene expression were analyzed,while histological analyses were performed after 2 and 8 wk.RESULTS The percentage of vascularization was significantly higher in BC compared to BPUI and BPEO constructs 2 and 8 wk after implantation.BC had the lowest endothelial-related gene expression,weaker osteocalcin immunoexpression and Spp1 expression compared to BPUI and BPEO.Endothelial-related gene expression and osteocalcin immunoexpression were higher in BPUI compared to BC and BPEO.BPEO had a higher percentage of vascularization compared to BPUI and the highest CD31 immunoexpression among examined constructs.Except Vwf,endothelial-related gene expression in BPEO had a later onset and was upregulated and well-balanced during in vivo incubation that induced late onset of Spp1 expression and pronounced osteocalcin immunoexpression at 2 and 8 wk.Tissue regression was noticed in BPEO constructs after 8 wk.CONCLUSION Ectopically implanted BPEO constructs had a favorable impact on vascularization and osteogenesis,but tissue regression imposed the need for discovering a more optimal EC/OB ratio prior to considerations for clinical applications.展开更多
Despite an increase in spectrum of industrial applications of yttrium(Y) and the fact that it is widely present in the soils and plants,some of which are agronomically important crops,its effects on plant growth and m...Despite an increase in spectrum of industrial applications of yttrium(Y) and the fact that it is widely present in the soils and plants,some of which are agronomically important crops,its effects on plant growth and metabolism are still obscure.Therefore,the aim of this work was to examine the effect of different concentrations of Y on its accumulation and distribution,photosynthetic responses,water relations,free proline concentration and growth of young maize plants.The experiment was done with maize(Zea mays L.,hybrid NS-640),in water cultures,under semi-controlled conditions of a greenhouse.Plants were supplied with half-strength complete Hoagland nutrient solution,to which was added either 0(control),10–5,10–4 or 10–3 mol/L Y,in the form of Y(NO)3·5H2O.Each variant was set in thirteen replications,with six plants in each replication.Plants were grown for 21 d and they were at the stage of 3 and 4 leaves when they were analyzed.The presence of Y reduced maize growth and photosynthetic performance.Dimensions of stomata significantly decreased while their density significantly increased on both adaxial and abaxial epidermis.Plant height,root length,total leaf area and dry mass also declined.Concentration of photosynthetic pigments(chl a and b and carotenoids) and free proline decreased.Photosynthesis and transpiration were impaired in the presence of Y – their intensities were also reduced,and the same stands for stomatal conductance of water vapor,photosynthetic water use efficiency(WUE) and water content.Although the highest concentration of Y was found in maize roots in each treatment,Y concentration in the second leaf and shoot also significantly increased with an increase in Y concentration in the nutrient solution.Albeit Y concentration was much higher in roots than in shoots,shoot metabolism and growth were much more disrupted.The results demonstrated that young maize plants accumulated significant amount of Y and that this element,when present in higher concentrations,had unfavorable effect on physiological processes and therefore plant growth.展开更多
Manufacturing execution systems(MESs)play a significant role in the manufacturing paradigm.MES is there to link between the Enterprise Resource Planning(ERP)systems and the plant equipment control or Supervisory Contr...Manufacturing execution systems(MESs)play a significant role in the manufacturing paradigm.MES is there to link between the Enterprise Resource Planning(ERP)systems and the plant equipment control or Supervisory Control and Data Acquisition(SCADA)applications.In this paper the MES of the INAPRO aquaponics system which was developed to support and advise the aquaponics managers in operating the complex aquaponic farms,will be presented.One important feature of the INAPRO aquaponics system is to minimize fresh water<3%,energy and nutrient supplies.This can only be achieved by appropriate design of the fish and crop mixture,considering the fish to crop ratio,when to sow the crops etc.and to monitor the system to see whether it performing as designed or not.Therefore,the MES has a view to show the designed system with all the material flow(water,energy and nutrients)balances and also how the system will be performing for a given predictive horizon.Knowing the future developments of the system,the operator can taking corrective measures to make sure that the system is behaving as required.An example of water balance of a system with 40 m3 fish tanks coupled with a hydroponic NFT system with 1,000 m2 which can produce five tons of Tilapia and 75 tons of tomato yearly is given.展开更多
Cyanobacteria are known to be a rich source of biologically active compounds some of which can have pharmaceutical importance. In this work we present the screening results of cyanobacterial strains for their antibact...Cyanobacteria are known to be a rich source of biologically active compounds some of which can have pharmaceutical importance. In this work we present the screening results of cyanobacterial strains for their antibacterial, antifungal, and cytotoxic activity. Cyanobacterial strains were isolated from various soil types in province of Vojvodina and Central Serbia, Republic of Serbia. The screening included 9 strains of Anabaena and 9 strains of Nostoc. Both, extracellular products (from the culture liquid) and cellular crude lipophilic extracts were tested against 13 bacterial strains and 8 fungal strains. Cytotoxic activity was tested against three human cell lines. Methanol extracts were prepared according to ?stensvik. Antibacterial and antifungal activities were determined measuring inhibition zone, 48 h after inoculation. The cytotoxic activity was determined by sulforhodamine B (SRB) colorimetric assay. Of all cyanobacterial strains tested, 52% showed some antifungal and 41% antibacterial activity. Two out of six tested strains possessed cytotoxic activity. The cytotoxic activity of Anabaena strain S12 was found both in culture liquid and crude cell extract. It occurred specifically between the 21st and 42nd day of cultivation against HeLa and MCF7 cells, but had no activity against cell line derived from a healthy tissue. A high percentage of the active strains among the tested strains justify the effort of screening cyanobacteria that are isolated from terrestrial environments. The most promising strains for the fur- ther study are Anabaena strain S12 which showed strong cytotoxic and antibacterial activity and Ana- baena strain S20 which produces a potent antifungal compound. The future work, besides further screening and chemical identification of the active compounds, should also include the development of culture techniques that would lead to more efficient production of biologically active compounds.展开更多
The retrospective study (2002-2007) for human leptospirosis in Vojvodina was undertaken in order to describe the distribution of the disease in relation with some environmental factors. Regarding the presented results...The retrospective study (2002-2007) for human leptospirosis in Vojvodina was undertaken in order to describe the distribution of the disease in relation with some environmental factors. Regarding the presented results, the major detected number of leptospirosis cases concurs with stagnant waters, wetlands, fish pond areas and protected regions, which comprised the basis for mapping of the region in three risk zones: very high risk (incidence rate higher than 5.0), high risk (2.5-5.0) and medium risk of leptospirosis infection (1.0-2.5). During the investigated period, 97 cases were registered with an average of 13.85 cases per year: 2002, 32 cases; 2003, 7; 2004, 22; 2005, 16; 2006, 4 and 2007, 16. Out of these 97 cases only 5 were women. Serovars from 11 presumptive serogroups caused infection, with a predominance of Icterohaemorrhagiae and Bratislava, accounting for 72.72% of cases together. Icterohaemorrhagiae was the commonest infecting serogroup mostly connected with fish ponds. Case fatality ratio was 9.4%.展开更多
Consistent inter-individual differences in daily activity rhythms(i.e.,chronotypes)can have ecological consequences in determining access to food resources and avoidance of predators.The most common measure to charact...Consistent inter-individual differences in daily activity rhythms(i.e.,chronotypes)can have ecological consequences in determining access to food resources and avoidance of predators.The most common measure to characterize chronotypes in animals as well as humans is the onset of activity(i.e.,early or late chronotypes).However,daily activity rhythms may also differ in the relative amount of activity displayed at particular time periods.Moreover,chronotypes may also be linked to other consistent inter-individual differences in behavior(i.e.,personality),such as the propensity to take risks.Here,we used the signal crayfish Pacifastacus leniusculusto test the presence of chronotypes and risk-taking personality traits and a potential behavioral syndrome between these traits.We first exposed crayfish to 5 days of light-darkness to measure daily activity rhythms and then we applied a visual predator-simulating stimulus in 2 different contexts(neutral and food).Our results showed consistent(i.e.,across 5 days)inter-individual differences in the relative nocturnal activity displayed in the early and middle,but not in the late part of darkness hours.Moreover,while crayfish displayed inter-individual differences in risk-taking behavior,these were not found to be consistent across 2 contexts.Therefore,we were not able to formally test a behavioral syndrome between these 2 traits.In conclusion,our study provides the first evidence of chronotypes in the relative amount of activity displayed at particular time periods.This could be a valuable information for applied ecological aspects related to the signal crayfish,which is a major invasive species of freshwater ecosystems.展开更多
基金Supported by the Ministry of Education,Science and Technological Development of the Republic of Serbia,451-03-68/2022-14/200125Provincial Secretariat for Higher Education and Scientific Research,AP Vojvodina,Republic of Serbia,142-451-3120/2022-01.
文摘The number of people diagnosed with diabetes continues to increase,especially among younger populations.Apart from genetic predisposition and lifestyle,there is increasing scientific and public concern that environmental agents may also contribute to diabetes.Food contamination by chemical substances that originate from packaging materials,or are the result of chemical reactions during food processing,is generally recognized as a worldwide problem with potential health hazards.Phthalates,bisphenol A(BPA)and acrylamide(AA)have been the focus of attention in recent years,due to the numerous adverse health effects associated with their exposure.This paper summarizes the available data about the association between phthalates,BPA and AA exposure and diabetes.Although their mechanism of action has not been fully clarified,in vitro,in vivo and epidemiological studies have made significant progress toward identifying the potential roles of phthalates,BPA and AA in diabetes development and progression.These chemicals interfere with multiple signaling pathways involved in glucose and lipid homeostasis and can aggravate the symptoms of diabetes.Especially concerning are the effects of exposure during early stages and the gestational period.Well-designed prospective studies are needed in order to better establish prevention strategies against the harmful effects of these food contaminants.
基金Supported by Ministry of Higher Education Scientific Research and Technology,Algeria(Grant No.F03120130013/2014)
文摘Objective:To investigate the total phenolics,flavonoids and tannins content and the in vitro antioxidant activities of methanolic extracts of six wild Mentha species which are Mentha aquatica,Mentha arvensis,Mentha piperita,Mentha pulegium,Mentha rotundifolia and Mentha villosa.Methods:The Folin–Ciocalteu method was used to determine the total phenols content while flavonoids were estimated according to the aluminum chloride colorimetric method.To evaluate tannins content,vanillin and HCl were added to methanolic extracts.The antioxidant potential was measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging,ferrous ion chelating and the inhibition of b-carotene bleaching assays.Results:The methanol extracts of Algerian mints were rich in phenolic compounds and exhibited powerful antioxidant activity ranging from 7.5 mg/mL to 44.66 mg/mL,which varied significantly among species.Mentha aquatica stood out with efficient antioxidant ability which was correlated to the high total phenolics content,followed by Mentha arvensis and Mentha piperita with very close values,comparing to Mentha pulegium,Mentha rotundifolia and Mentha villosa with lowest values.Conclusions:These results show that methanolic extracts of Mentha species from Algeria have a great potential of polyphenols which can be used as a natural food preservative and antioxidant source.
基金supported by the Ministry of Education,Science and Technological Development of the Republic of Serbia,grant OI 172058.
文摘Objective:To examine the effect of Rumex crispus(R.crispus)and Rumex sanguineus(R.sanguineus)plant extracts against isolates of Acinetobacter baumannii(A.baumannii)from wounds,including multidrug-resistant strains.Methods:Six prepared Rumex extracts were subjected to liquid chromatography-tandem mass spectrometry.Antimicrobial activity of extracts and pure compounds(catechin,quercetin,isoquercitrin,emodin,and gallic acid)was examined by a microtiter plate method,while for determination of compound binary combinations activity a checkerboard method was applied.Active fractions of extracts were detected by agar-overlay high-performance thinlayer chromatography-bioautography assay followed by liquid chromatography-diode array detection-mass spectrometry analysis.Results:A total of 28 compounds were detected in two extracts of R.crispus and 26 compounds in four different R.sanguineus extracts,with catechin as a dominant component.Anti-A.baumannii activity was confirmed for all six R.sanguineus and R.crispus extracts at the concentration range from 1 to 4 mg/mL.Neither examined single compounds nor their binary combinations exhibited an anti-A.baumannii activity(MIC>256μg/mL).The bioautography showed that fractions with the most prominent anti-A.baumannii activity tended to contain more polar compounds,predominantly flavonol(quercetin and kaempherol)glycosides;but also fractions containing flavanone(eriodictyol)glycosides and anthraquinone(emodin)glycosides;and less polar eriodictyol aglycone.Conclusions:The results justify and elucidate the traditional application of R.sanguineus and R.crispus extracts for wound healing,indicating the necessity for their further examination in combat against multidrug-resistant A.baumannii isolates from wounds.
基金supported by Research Grant No. NN304060339 from the Ministry of Science and Higher Education of Poland
文摘Inter Simple Sequence Repeats(ISSR) markers were used to assess genetic diversity within and among populations of dwarf mountain pine(Pinus mugo Turra) growing in the Tatra National Park(UNESCO Biosphere Reserve) in Southern Poland(Central Europe). The analyzed population belongs to two different geobotanical sub-districts: the Western and High Tatras. The level of genetic diversity assessed in this study for P. mugo is generally comparable to that reported for the other pine species in the Pinaceae family assessed by ISSR markers, especially with respect to Nei's genetic diversity and the percentage of polymorphic bands. Bayesian analysis clustered the analyzed populations into two groups, corresponding to their geobotanical locations in the Tatras. Significant divergence between the two genetical clusters was supported by the results of Analysis of Molecular Variance(AMOVA). According to the Mantel test, there was no correlation between the genetic distance and the geographical distance. The present study confirms the existence of two genetically distinct clusters of P. mugo populations in the Tatra Mountains. The observed high population-genetic differentiation of P.mugo in the Tatras could be attributed to several genetic, environmental and historical factors occurring in this mountain area.
文摘For the first time, the anatomical structure of the vegetative organs of the species J. hippolyti and J. narbuti was studied in the conditions of Kyzylkum (Uzbekistan, Province Bukhara) and revealed the diagnostic features of these species. A different combination of xeromorphic and mesomorphic features was determined, which ensures adaptation to habitat conditions. In J. narbuti xeromorphic features predominate, which indicates a more adaptable and wide distribution in the natural habitat of this species. In J. hippolyti—mesomorphic signs that show the rarity of the species in their natural habitat, compared to J. narbuti.
文摘Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His? TA102 strain. Inhibition values of 44.2% and 67.0% were detected for t-BHP and H2O2, respectively. A protective ability of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in plasmid DNA showed a significant inhibition of DNA damage.
基金Supported by Ministry of Education,Science and Technological Development of the Republic of Serbia,No.III 41017.
文摘BACKGROUND A major problem in the healing of bone defects is insufficient or absent blood supply within the defect.To overcome this challenging problem,a plethora of approaches within bone tissue engineering have been developed recently.Bearing in mind that the interplay of various diffusible factors released by endothelial cells(ECs)and osteoblasts(OBs)have a pivotal role in bone growth and regeneration and that adjacent ECs and OBs also communicate directly through gap junctions,we set the focus on the simultaneous application of these cell types together with platelet-rich plasma(PRP)as a growth factor reservoir within ectopic bone tissue engineering constructs.AIM To vascularize and examine osteogenesis in bone tissue engineering constructs enriched with PRP and adipose-derived stem cells(ASCs)induced into ECs and OBs.METHODS ASCs isolated from adipose tissue,induced in vitro into ECs,OBs or just expanded were used for implant construction as followed:BPEO,endothelial and osteogenic differentiated ASCs with PRP and bone mineral matrix;BPUI,uninduced ASCs with PRP and bone mineral matrix;BC(control),only bone mineral matrix.At 1,2,4 and 8 wk after subcutaneous implantation in mice,implants were extracted and endothelial-related and bone-related gene expression were analyzed,while histological analyses were performed after 2 and 8 wk.RESULTS The percentage of vascularization was significantly higher in BC compared to BPUI and BPEO constructs 2 and 8 wk after implantation.BC had the lowest endothelial-related gene expression,weaker osteocalcin immunoexpression and Spp1 expression compared to BPUI and BPEO.Endothelial-related gene expression and osteocalcin immunoexpression were higher in BPUI compared to BC and BPEO.BPEO had a higher percentage of vascularization compared to BPUI and the highest CD31 immunoexpression among examined constructs.Except Vwf,endothelial-related gene expression in BPEO had a later onset and was upregulated and well-balanced during in vivo incubation that induced late onset of Spp1 expression and pronounced osteocalcin immunoexpression at 2 and 8 wk.Tissue regression was noticed in BPEO constructs after 8 wk.CONCLUSION Ectopically implanted BPEO constructs had a favorable impact on vascularization and osteogenesis,but tissue regression imposed the need for discovering a more optimal EC/OB ratio prior to considerations for clinical applications.
基金Project supported by Ministry of Education,Science and Technological Development of the Republic of Serbia
文摘Despite an increase in spectrum of industrial applications of yttrium(Y) and the fact that it is widely present in the soils and plants,some of which are agronomically important crops,its effects on plant growth and metabolism are still obscure.Therefore,the aim of this work was to examine the effect of different concentrations of Y on its accumulation and distribution,photosynthetic responses,water relations,free proline concentration and growth of young maize plants.The experiment was done with maize(Zea mays L.,hybrid NS-640),in water cultures,under semi-controlled conditions of a greenhouse.Plants were supplied with half-strength complete Hoagland nutrient solution,to which was added either 0(control),10–5,10–4 or 10–3 mol/L Y,in the form of Y(NO)3·5H2O.Each variant was set in thirteen replications,with six plants in each replication.Plants were grown for 21 d and they were at the stage of 3 and 4 leaves when they were analyzed.The presence of Y reduced maize growth and photosynthetic performance.Dimensions of stomata significantly decreased while their density significantly increased on both adaxial and abaxial epidermis.Plant height,root length,total leaf area and dry mass also declined.Concentration of photosynthetic pigments(chl a and b and carotenoids) and free proline decreased.Photosynthesis and transpiration were impaired in the presence of Y – their intensities were also reduced,and the same stands for stomatal conductance of water vapor,photosynthetic water use efficiency(WUE) and water content.Although the highest concentration of Y was found in maize roots in each treatment,Y concentration in the second leaf and shoot also significantly increased with an increase in Y concentration in the nutrient solution.Albeit Y concentration was much higher in roots than in shoots,shoot metabolism and growth were much more disrupted.The results demonstrated that young maize plants accumulated significant amount of Y and that this element,when present in higher concentrations,had unfavorable effect on physiological processes and therefore plant growth.
基金This research was supported by the European Union’s Seventh Framework Programme FP7-ENV-2013-WATERINNO-DEMO under Grant agreement N619137.
文摘Manufacturing execution systems(MESs)play a significant role in the manufacturing paradigm.MES is there to link between the Enterprise Resource Planning(ERP)systems and the plant equipment control or Supervisory Control and Data Acquisition(SCADA)applications.In this paper the MES of the INAPRO aquaponics system which was developed to support and advise the aquaponics managers in operating the complex aquaponic farms,will be presented.One important feature of the INAPRO aquaponics system is to minimize fresh water<3%,energy and nutrient supplies.This can only be achieved by appropriate design of the fish and crop mixture,considering the fish to crop ratio,when to sow the crops etc.and to monitor the system to see whether it performing as designed or not.Therefore,the MES has a view to show the designed system with all the material flow(water,energy and nutrients)balances and also how the system will be performing for a given predictive horizon.Knowing the future developments of the system,the operator can taking corrective measures to make sure that the system is behaving as required.An example of water balance of a system with 40 m3 fish tanks coupled with a hydroponic NFT system with 1,000 m2 which can produce five tons of Tilapia and 75 tons of tomato yearly is given.
基金the Ministry of Science and Environmental Protection of the Republic of Serbia (GrantNo.146021B)
文摘Cyanobacteria are known to be a rich source of biologically active compounds some of which can have pharmaceutical importance. In this work we present the screening results of cyanobacterial strains for their antibacterial, antifungal, and cytotoxic activity. Cyanobacterial strains were isolated from various soil types in province of Vojvodina and Central Serbia, Republic of Serbia. The screening included 9 strains of Anabaena and 9 strains of Nostoc. Both, extracellular products (from the culture liquid) and cellular crude lipophilic extracts were tested against 13 bacterial strains and 8 fungal strains. Cytotoxic activity was tested against three human cell lines. Methanol extracts were prepared according to ?stensvik. Antibacterial and antifungal activities were determined measuring inhibition zone, 48 h after inoculation. The cytotoxic activity was determined by sulforhodamine B (SRB) colorimetric assay. Of all cyanobacterial strains tested, 52% showed some antifungal and 41% antibacterial activity. Two out of six tested strains possessed cytotoxic activity. The cytotoxic activity of Anabaena strain S12 was found both in culture liquid and crude cell extract. It occurred specifically between the 21st and 42nd day of cultivation against HeLa and MCF7 cells, but had no activity against cell line derived from a healthy tissue. A high percentage of the active strains among the tested strains justify the effort of screening cyanobacteria that are isolated from terrestrial environments. The most promising strains for the fur- ther study are Anabaena strain S12 which showed strong cytotoxic and antibacterial activity and Ana- baena strain S20 which produces a potent antifungal compound. The future work, besides further screening and chemical identification of the active compounds, should also include the development of culture techniques that would lead to more efficient production of biologically active compounds.
基金Surpported by the funding of the European Agency for Reconstruction Through the Ministry of International Economic Relations within the Neighboring Program Hungary-Serbia (Grant No. 04SER02/01/009)Ministry of Science and Environmental Protection of the Republic of Serbia (Grant No. 146021B)
文摘The retrospective study (2002-2007) for human leptospirosis in Vojvodina was undertaken in order to describe the distribution of the disease in relation with some environmental factors. Regarding the presented results, the major detected number of leptospirosis cases concurs with stagnant waters, wetlands, fish pond areas and protected regions, which comprised the basis for mapping of the region in three risk zones: very high risk (incidence rate higher than 5.0), high risk (2.5-5.0) and medium risk of leptospirosis infection (1.0-2.5). During the investigated period, 97 cases were registered with an average of 13.85 cases per year: 2002, 32 cases; 2003, 7; 2004, 22; 2005, 16; 2006, 4 and 2007, 16. Out of these 97 cases only 5 were women. Serovars from 11 presumptive serogroups caused infection, with a predominance of Icterohaemorrhagiae and Bratislava, accounting for 72.72% of cases together. Icterohaemorrhagiae was the commonest infecting serogroup mostly connected with fish ponds. Case fatality ratio was 9.4%.
基金V.S.was supported by a Leibniz-DAAD Postdoctoral Research Fellowship(no.91632699)while he is now supported by the Spanish Ministry of Science and Innovation with a “Juan de la Cierva Incorporacion”research fellowship(IJC2018-035389-1)he acknowledges the “Severo Ochoa Centre of Excellence”accreditation(CEX2019-000928-S).
文摘Consistent inter-individual differences in daily activity rhythms(i.e.,chronotypes)can have ecological consequences in determining access to food resources and avoidance of predators.The most common measure to characterize chronotypes in animals as well as humans is the onset of activity(i.e.,early or late chronotypes).However,daily activity rhythms may also differ in the relative amount of activity displayed at particular time periods.Moreover,chronotypes may also be linked to other consistent inter-individual differences in behavior(i.e.,personality),such as the propensity to take risks.Here,we used the signal crayfish Pacifastacus leniusculusto test the presence of chronotypes and risk-taking personality traits and a potential behavioral syndrome between these traits.We first exposed crayfish to 5 days of light-darkness to measure daily activity rhythms and then we applied a visual predator-simulating stimulus in 2 different contexts(neutral and food).Our results showed consistent(i.e.,across 5 days)inter-individual differences in the relative nocturnal activity displayed in the early and middle,but not in the late part of darkness hours.Moreover,while crayfish displayed inter-individual differences in risk-taking behavior,these were not found to be consistent across 2 contexts.Therefore,we were not able to formally test a behavioral syndrome between these 2 traits.In conclusion,our study provides the first evidence of chronotypes in the relative amount of activity displayed at particular time periods.This could be a valuable information for applied ecological aspects related to the signal crayfish,which is a major invasive species of freshwater ecosystems.
