ObjectiveTo study the effects of dendritic cells (DC) transfected with recombinant vaccinia virus encoding Epstein Barr virus (EBV) latent membrane protein 2A(LMP2A) gene,and to provide evidence for further investiga...ObjectiveTo study the effects of dendritic cells (DC) transfected with recombinant vaccinia virus encoding Epstein Barr virus (EBV) latent membrane protein 2A(LMP2A) gene,and to provide evidence for further investigation on the therapeutic vaccines against EBV associated malignancies. MethodsMature DC were transfected with EBV LMP2A recombinant vaccinia virus (rVV LMP2A). Before and after the transfection,the expression of surface antigens on mature DC including CD1a,CD83,CD40,CD80,HLA DR was measured by fluorescence activated cell sorter (FACS) and the function of DC to stimulate allogeneic T cells proliferation was measured by mixed leukocyte reactions (MLR). ResultsLMP2A protein was highly expressed (66.1 %) in DC after the transfection of rVV LMP2A. No significant changes in the primary surface antigens expression and in the MLR were detected during the transfection. Transfected DC still had strong potential in stimulating the proliferation of allogeneic T cells. ConclusionRecombinant vaccinia virus was an effective and non perturbing vector to mediate the transfection of LMP2A into DC. The functions of mature DC were not affected significantly by the transfection of Vac LMP2A. This study could provide evidence for the further immunotherapy of EBV associated malignancies,e.g. nasopharyngeal carcinoma (NPC).展开更多
In the history of medicine, relatively little attention has been paid to the way medical illustration circulated globally---or to the issues raised by the cultural ＂translation＂ of such images. My goal here is to fl...In the history of medicine, relatively little attention has been paid to the way medical illustration circulated globally---or to the issues raised by the cultural ＂translation＂ of such images. My goal here is to flesh out some of the history of this circulation and translation by exploring the aesthetic and medical connections be- tween two specific anatomical collections, both housed at the Gordon Pathology Museum at Guy＇s Hospital, London. The Joseph Towne collection of anatomical waxes and the Lam Qua paintings of the patients of medical missionary Peter Parker were both produced in the nineteenth century. Significantly, the two collections were part of related but culturally specific shifts in the way bodies （and diseased bodies） were viewed, represented, understood and treated. I explore some of the convergences and divergences between Western and Chinese medical and artistic priorities and will address some of the issues raised by them. These two collections are important, I argue, because they demonstrate how aesthetic considerations shape medical knowledge and wider attitudes about the human body.展开更多
We are delighted to be able to introduce this special issue of the Fudan Journal of the Humanities and Social Sciences, on behalf of the University of Exeter, the College of Humanities, and the Departments of English ...We are delighted to be able to introduce this special issue of the Fudan Journal of the Humanities and Social Sciences, on behalf of the University of Exeter, the College of Humanities, and the Departments of English and Modern Languages. This special issue selects four articles revised from papers delivered at the inaugural Fudan-Exeter Colloquium, Reading across Cultures： Global Configurations of Reception, Adaptation and Transmission, held at Fudan University, Shanghai, from 23 to 25 June, 2014. Twelve academics from Exeter were hosted by colleagues in Fudan, and over the course of 3 days, twenty-six papers were delivered in eight themed sessions, as well as two keynote lectures by Dean Chu （Linguistic diversity in trans-cultural communication） and Dean Kaye （Site Works： Archaeologies of Bay Area Performance, 1969-85）, respectively. The colloquium was a great success and cemented the relationship between Fudan and Exeter. We hope that it will be the first of many such collaborations between our institutions. In the globalised academic world of the twenty-first century, questions of reception and adaptation take on a new urgency. Scholars are investigating the ways in which the literature, visual, and cultural artefacts belonging to one time and place are received by new audiences and adapted by new practitioners, across geographical frontiers as well as across historical eras.展开更多
基金This paper is supported by grant from the National Natural Science Foundation of China(No.30 1 70 880 )
文摘ObjectiveTo study the effects of dendritic cells (DC) transfected with recombinant vaccinia virus encoding Epstein Barr virus (EBV) latent membrane protein 2A(LMP2A) gene,and to provide evidence for further investigation on the therapeutic vaccines against EBV associated malignancies. MethodsMature DC were transfected with EBV LMP2A recombinant vaccinia virus (rVV LMP2A). Before and after the transfection,the expression of surface antigens on mature DC including CD1a,CD83,CD40,CD80,HLA DR was measured by fluorescence activated cell sorter (FACS) and the function of DC to stimulate allogeneic T cells proliferation was measured by mixed leukocyte reactions (MLR). ResultsLMP2A protein was highly expressed (66.1 %) in DC after the transfection of rVV LMP2A. No significant changes in the primary surface antigens expression and in the MLR were detected during the transfection. Transfected DC still had strong potential in stimulating the proliferation of allogeneic T cells. ConclusionRecombinant vaccinia virus was an effective and non perturbing vector to mediate the transfection of LMP2A into DC. The functions of mature DC were not affected significantly by the transfection of Vac LMP2A. This study could provide evidence for the further immunotherapy of EBV associated malignancies,e.g. nasopharyngeal carcinoma (NPC).
文摘In the history of medicine, relatively little attention has been paid to the way medical illustration circulated globally---or to the issues raised by the cultural ＂translation＂ of such images. My goal here is to flesh out some of the history of this circulation and translation by exploring the aesthetic and medical connections be- tween two specific anatomical collections, both housed at the Gordon Pathology Museum at Guy＇s Hospital, London. The Joseph Towne collection of anatomical waxes and the Lam Qua paintings of the patients of medical missionary Peter Parker were both produced in the nineteenth century. Significantly, the two collections were part of related but culturally specific shifts in the way bodies （and diseased bodies） were viewed, represented, understood and treated. I explore some of the convergences and divergences between Western and Chinese medical and artistic priorities and will address some of the issues raised by them. These two collections are important, I argue, because they demonstrate how aesthetic considerations shape medical knowledge and wider attitudes about the human body.
文摘We are delighted to be able to introduce this special issue of the Fudan Journal of the Humanities and Social Sciences, on behalf of the University of Exeter, the College of Humanities, and the Departments of English and Modern Languages. This special issue selects four articles revised from papers delivered at the inaugural Fudan-Exeter Colloquium, Reading across Cultures： Global Configurations of Reception, Adaptation and Transmission, held at Fudan University, Shanghai, from 23 to 25 June, 2014. Twelve academics from Exeter were hosted by colleagues in Fudan, and over the course of 3 days, twenty-six papers were delivered in eight themed sessions, as well as two keynote lectures by Dean Chu （Linguistic diversity in trans-cultural communication） and Dean Kaye （Site Works： Archaeologies of Bay Area Performance, 1969-85）, respectively. The colloquium was a great success and cemented the relationship between Fudan and Exeter. We hope that it will be the first of many such collaborations between our institutions. In the globalised academic world of the twenty-first century, questions of reception and adaptation take on a new urgency. Scholars are investigating the ways in which the literature, visual, and cultural artefacts belonging to one time and place are received by new audiences and adapted by new practitioners, across geographical frontiers as well as across historical eras.
