Objective:To investigate the anti-senescence effect of 3-bromo-4,5-dihydroxybenzaldehyde(BDB)from Polysiphonia morrowii Harvey in human dermal fibroblasts(HDF).Methods:HDF were subjected to treatment of BDB and then t...Objective:To investigate the anti-senescence effect of 3-bromo-4,5-dihydroxybenzaldehyde(BDB)from Polysiphonia morrowii Harvey in human dermal fibroblasts(HDF).Methods:HDF were subjected to treatment of BDB and then treated with hydrogen peroxide(H2O2)to induce premature senescence.Senescence-associatedβ-galactosidase(SA-β-gal)activity in HDF was determined using the SA-β-gal staining method.Intracellular reactive oxygen species(ROS)production was measured using the 2’,7’-dichlorodihydrofluorescein diacetate assay.Western blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1(GPX1).In addition,intracellular collagen and collagenase contents were analyzed using the respective ELISA kits.Elastase activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein content.Results:Treatment of HDF with H2O2 increased the activity of SA-β-gal,but BDB pre-treatment resulted in the reduction of SA-β-gal activity.Moreover,BDB significantly reduced H2O2-induced intracellular ROS production.BDB also markedly increased the level of GPX1,which was inhibited by 400μM of H2O2.Furthermore,in in vitro study,BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in HDF.Conclusions:Our results demonstrate that BDB shows antisenescence and anti-wrinkle activities in vitro.展开更多
Objective: To evaluate the in vitro antimicrobial property of three different partitioned extracts(petroleum ether, ethanol and water) prepared from some fungal mycelia.Methods: Seven fungal mycelia were prepared, ini...Objective: To evaluate the in vitro antimicrobial property of three different partitioned extracts(petroleum ether, ethanol and water) prepared from some fungal mycelia.Methods: Seven fungal mycelia were prepared, initially extracted with acidified ethanol(0.2 mol/L HCl in 80% ethanol), yielding the raw crude extracts. The obtained extracts were then further partitioned with petroleum ether(F1), ethanol(F2) and water(F3). All the fractions were tested for antimicrobial activity using the disc diffusion assay.Results: Our data showed that all the fractions could inhibit the testing bacteria.However, the inhibitory activity was found to be dependent on(i) the fungal strains used;(ii) the solvent extracted; and(iii) the testing bacteria assayed. In general, the ethanolic extracts(F2) derived from all fungi displayed highest inhibitory activity against the testing bacteria except for Chaetomium sp.Conclusions: The findings of the present study concluded that the extracts prepared from the fungal mycelia had the bioactive compounds with antibacterial property. This study is a pioneering work and further study should be carried out for development of the new drug leads.展开更多
Objective:To demonstrate the effect of dieckol from Eisenia bicyclis on osteoclastogenesis using RAW 264.7 cells.Methods:Murine macrophage RAW 264.7 cells were subjected to dieckol treatment,followed by treatment with...Objective:To demonstrate the effect of dieckol from Eisenia bicyclis on osteoclastogenesis using RAW 264.7 cells.Methods:Murine macrophage RAW 264.7 cells were subjected to dieckol treatment,followed by treatment with receptor activator of nuclear factor kappa-B ligand(RANKL)to induce osteoclastogenesis.Tartrate-resistant acid phosphatase(TRAP)activity was examined using a TRAP activity kit.Western blotting analysis was conducted to examine the level of osteoclast-related factors,including TRAP and calcitonin receptor(CTR),transcriptional factors,including c-Fos,c-Jun,and nuclear factor of activated T cells cytoplasmic 1(NFATc1),nuclear factor kappa-B(NF-κB),extracellular signal-regulated kinase(ERK),and c-Jun N-terminal kinase(JNK).Immunofluorescence staining was conducted to examine the expression of c-Fos,c-Jun,and NFATc1.Results:Among the four phlorotannin compounds present in Eisenia bicyclis,dieckol significantly hindered osteoclast differentiation and expression of RANKL-induced TRAP and CTR.In addition,dieckol downregulated the expression levels of c-Fos,c-Jun,NFATc1,ERK,and JNK,and suppressed NF-κB signaling.Conclusions:Dieckol can suppress RANKL-induced osteoclastogenesis.Therefore,it has therapeutic potential in treating osteoclastogenesis-associated diseases.展开更多
This study represents a well-dispersed gold nanoparticles(AuNPs)synthesis process via cellulose nanocrystals(CNC)which acts as both reducing and supporting agent.The synthesis process was ultrafast and completed in a ...This study represents a well-dispersed gold nanoparticles(AuNPs)synthesis process via cellulose nanocrystals(CNC)which acts as both reducing and supporting agent.The synthesis process was ultrafast and completed in a few seconds using microwave irradiation.The entire synthesis process was cost-effective,sustainable and eco-friendly.The synthesized(AuNPs/CNC)nanocomposite was investigated by transmission electron microscopy,selected area electron diffraction,Fourier transform infrared spectroscopy,energy dispersive X-ray analysis,X-ray diffraction,and UV–vis spectroscopy.The obtained AuNPs were well accumulated on the CNC surface and had a uniform spherical shape with an average diameter of 8±5.3 nm.The diameter of AuNPs could be altered by tuning the concentration of CNC suspension.The synthesized AuNPs/CNC nanocomposite film exhibited excellent degradation properties against various organic dyes,namely,Allura red,Congo red,Rhodamine B and Amaranth.The ultrafast degradation reactions followed pseudo first order kinetics.In the catalytic degradation reaction,AuNPs/CNC was transmitting electrons from a donor(NaBH4)to an acceptor(a dye).展开更多
基金supported by Korea Basic Science Institute(grant number C39260)National Research Foundation of Korea(NRF)funded by the Korea government(MSIT)(grant number NRF-2019R1C1C1005608)a research grant from the Korea Institute of Ocean Science and Technology(PE99822)
文摘Objective:To investigate the anti-senescence effect of 3-bromo-4,5-dihydroxybenzaldehyde(BDB)from Polysiphonia morrowii Harvey in human dermal fibroblasts(HDF).Methods:HDF were subjected to treatment of BDB and then treated with hydrogen peroxide(H2O2)to induce premature senescence.Senescence-associatedβ-galactosidase(SA-β-gal)activity in HDF was determined using the SA-β-gal staining method.Intracellular reactive oxygen species(ROS)production was measured using the 2’,7’-dichlorodihydrofluorescein diacetate assay.Western blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1(GPX1).In addition,intracellular collagen and collagenase contents were analyzed using the respective ELISA kits.Elastase activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein content.Results:Treatment of HDF with H2O2 increased the activity of SA-β-gal,but BDB pre-treatment resulted in the reduction of SA-β-gal activity.Moreover,BDB significantly reduced H2O2-induced intracellular ROS production.BDB also markedly increased the level of GPX1,which was inhibited by 400μM of H2O2.Furthermore,in in vitro study,BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in HDF.Conclusions:Our results demonstrate that BDB shows antisenescence and anti-wrinkle activities in vitro.
基金Supported by“Operational Program Prague–Competitiveness”(CZ.2.16/3.1.00/24503)the“National Program of Sustainability I”-NPU I(LO1601-No.:MSMT-43760/2015)from the University of Chemistry and Technology,Czech Republic
文摘Objective: To evaluate the in vitro antimicrobial property of three different partitioned extracts(petroleum ether, ethanol and water) prepared from some fungal mycelia.Methods: Seven fungal mycelia were prepared, initially extracted with acidified ethanol(0.2 mol/L HCl in 80% ethanol), yielding the raw crude extracts. The obtained extracts were then further partitioned with petroleum ether(F1), ethanol(F2) and water(F3). All the fractions were tested for antimicrobial activity using the disc diffusion assay.Results: Our data showed that all the fractions could inhibit the testing bacteria.However, the inhibitory activity was found to be dependent on(i) the fungal strains used;(ii) the solvent extracted; and(iii) the testing bacteria assayed. In general, the ethanolic extracts(F2) derived from all fungi displayed highest inhibitory activity against the testing bacteria except for Chaetomium sp.Conclusions: The findings of the present study concluded that the extracts prepared from the fungal mycelia had the bioactive compounds with antibacterial property. This study is a pioneering work and further study should be carried out for development of the new drug leads.
基金supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSITNo. NRF-2020R1A2C1008527)
文摘Objective:To demonstrate the effect of dieckol from Eisenia bicyclis on osteoclastogenesis using RAW 264.7 cells.Methods:Murine macrophage RAW 264.7 cells were subjected to dieckol treatment,followed by treatment with receptor activator of nuclear factor kappa-B ligand(RANKL)to induce osteoclastogenesis.Tartrate-resistant acid phosphatase(TRAP)activity was examined using a TRAP activity kit.Western blotting analysis was conducted to examine the level of osteoclast-related factors,including TRAP and calcitonin receptor(CTR),transcriptional factors,including c-Fos,c-Jun,and nuclear factor of activated T cells cytoplasmic 1(NFATc1),nuclear factor kappa-B(NF-κB),extracellular signal-regulated kinase(ERK),and c-Jun N-terminal kinase(JNK).Immunofluorescence staining was conducted to examine the expression of c-Fos,c-Jun,and NFATc1.Results:Among the four phlorotannin compounds present in Eisenia bicyclis,dieckol significantly hindered osteoclast differentiation and expression of RANKL-induced TRAP and CTR.In addition,dieckol downregulated the expression levels of c-Fos,c-Jun,NFATc1,ERK,and JNK,and suppressed NF-κB signaling.Conclusions:Dieckol can suppress RANKL-induced osteoclastogenesis.Therefore,it has therapeutic potential in treating osteoclastogenesis-associated diseases.
基金supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(No.2018R1A6A1A03025582).
文摘This study represents a well-dispersed gold nanoparticles(AuNPs)synthesis process via cellulose nanocrystals(CNC)which acts as both reducing and supporting agent.The synthesis process was ultrafast and completed in a few seconds using microwave irradiation.The entire synthesis process was cost-effective,sustainable and eco-friendly.The synthesized(AuNPs/CNC)nanocomposite was investigated by transmission electron microscopy,selected area electron diffraction,Fourier transform infrared spectroscopy,energy dispersive X-ray analysis,X-ray diffraction,and UV–vis spectroscopy.The obtained AuNPs were well accumulated on the CNC surface and had a uniform spherical shape with an average diameter of 8±5.3 nm.The diameter of AuNPs could be altered by tuning the concentration of CNC suspension.The synthesized AuNPs/CNC nanocomposite film exhibited excellent degradation properties against various organic dyes,namely,Allura red,Congo red,Rhodamine B and Amaranth.The ultrafast degradation reactions followed pseudo first order kinetics.In the catalytic degradation reaction,AuNPs/CNC was transmitting electrons from a donor(NaBH4)to an acceptor(a dye).
