In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the micro- scope. And then the dermatop...In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the micro- scope. And then the dermatophytes were cultured on Sabouraud＇s dextrose agar （SDA）. The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction （PCR）, and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN soft- ware were used to analyze the sequences. To further determine highly homologous sequences, a phy- logenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosurn and Micro- sporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate Cll5LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to mi- crosporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR （JN650537, Cladosporium ） and Ascomycete sp. LB68A1A2 （AY770409, Ascomycete sp）.展开更多
基金supported by the Doctoral Scientific Research Fund of Hebei United University(3907101162)
文摘In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the micro- scope. And then the dermatophytes were cultured on Sabouraud＇s dextrose agar （SDA）. The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction （PCR）, and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN soft- ware were used to analyze the sequences. To further determine highly homologous sequences, a phy- logenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosurn and Micro- sporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate Cll5LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to mi- crosporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR （JN650537, Cladosporium ） and Ascomycete sp. LB68A1A2 （AY770409, Ascomycete sp）.
