Objective: To study the effect of Danshao Huaxian capsule (丹芍化纤胶囊,DSHX), a traditional Chinese medical prescription, on the expression of collagen (Col) Ⅰ, Ⅲ and cysteinyl aspartate specific proteases-3 (caspa...Objective: To study the effect of Danshao Huaxian capsule (丹芍化纤胶囊,DSHX), a traditional Chinese medical prescription, on the expression of collagen (Col) Ⅰ, Ⅲ and cysteinyl aspartate specific proteases-3 (caspase-3) in CCl4-induced hepatic fibrosis in rats. And also it is to explore the mechanism of DSHX in anti-fibrosis. Methods: Eighty male Wistar rats were randomly divided into the normal control group (A), the model group (B), the un-treated model group (C), the low-dose-DSHX treated group (D) and the high-dose-DSHX treated group (E). Except those in Group A, all the other rats were made into hepatic fibrotic models by comprehensive processes including subcutaneous injecting of CCl4, and feeding them with alcoholic high-fat and low-protein diet for 8 weeks. Then the two DSHX-treated groups were treated respectively with low dose (0.5 g/kg) and high dose (1.0 g/kg) DSHX capsule by gastrogavage everyday for 8 weeks. At the end of the experiment, the liver index, levels of hyaluronic acid (HA) and alanine amin-otransferase (ALT) in serum, degree of hepatic fibrosis, and urinary excretion of hydroxyproline (Hyp) were measured, and the expression of Col Ⅰ , Col Ⅲ and caspase-3 in liver tissues were detected respectively by immunohistochemistric technique. Results: Compared with those in Group B and C, the two DSHX treated groups showed that the liver index, levels of serum HA and ALT and severity of hepatic fibrosis were all significantly lower, the urinary excretion of Hyp was significantly higher; the Col Ⅰ and Col-Ⅲ expression was lower (Col Ⅰ :1. 23±1.14,1. 07±0. 96 vs 4.18±2. 26, 3. 22±1. 44, P<0. 01;Col Ⅲ : 1. 31±0. 69, , 1. 09± 0.58 vs 3.04±0.62,2.23±0.58, P<0.05). At the same time, the expression of caspase-3 in Group E was fewer than Group B and C in hepatocytes (3. 09±0. 65 vs 9. 60±2. 32, 8. 82 ±1. 45, P<0.01),but it was extensively expressed in fibrous septal cells(4.52±0.87 vs 1.69±0.23,2.98±0.36, P<0.01). Conclusion: DSHX capsule shows certain therapeutic effect on hepatic fibrosis in rats, and the mechanism might be related with reducing Col Ⅰ and Col Ⅲ deposition, inhibiting hepatocyte apoptosis and promoting fibrous septal cells (mainly the activated hepatic stellate cells) apoptosis.展开更多
Extracellular vesicles(EVs)are heterogeneous nano-sized vesicles of endocytic origin shed into blood and other body fluids such as urine,saliva,seminal fluid,ascites,amniotic liquid,synovial fluid,breast milk and cere...Extracellular vesicles(EVs)are heterogeneous nano-sized vesicles of endocytic origin shed into blood and other body fluids such as urine,saliva,seminal fluid,ascites,amniotic liquid,synovial fluid,breast milk and cerebrospinal fluid(CSF)by quite all cell types.EVs actively contribute to intercellular communication as they carry bioactive molecules that are selectively incorporated by the originating cell and are delivered to recipient cells over long and short distances(Simons and Raposo,2009).EVs can be divided into three main groups according to their size and cellular origin:1)exosomes(40–120 nm),that have an endocytic origin and are formed by inward budding of the limiting membrane of multivescicular bodies,which fuse with the plasma membrane and release exosomes into the extracellular space;2)microvesicles(50–1000 nm),budding directly off the plasma membrane;3)apoptotic bodies(>1000 nm),which are released during apoptosis.Besides originating via distinct processes,different subtypes of EVs carry different proteins within their membrane and luminal compartments that reflect the phenotype of the tissue of origin.展开更多
文摘Objective: To study the effect of Danshao Huaxian capsule (丹芍化纤胶囊,DSHX), a traditional Chinese medical prescription, on the expression of collagen (Col) Ⅰ, Ⅲ and cysteinyl aspartate specific proteases-3 (caspase-3) in CCl4-induced hepatic fibrosis in rats. And also it is to explore the mechanism of DSHX in anti-fibrosis. Methods: Eighty male Wistar rats were randomly divided into the normal control group (A), the model group (B), the un-treated model group (C), the low-dose-DSHX treated group (D) and the high-dose-DSHX treated group (E). Except those in Group A, all the other rats were made into hepatic fibrotic models by comprehensive processes including subcutaneous injecting of CCl4, and feeding them with alcoholic high-fat and low-protein diet for 8 weeks. Then the two DSHX-treated groups were treated respectively with low dose (0.5 g/kg) and high dose (1.0 g/kg) DSHX capsule by gastrogavage everyday for 8 weeks. At the end of the experiment, the liver index, levels of hyaluronic acid (HA) and alanine amin-otransferase (ALT) in serum, degree of hepatic fibrosis, and urinary excretion of hydroxyproline (Hyp) were measured, and the expression of Col Ⅰ , Col Ⅲ and caspase-3 in liver tissues were detected respectively by immunohistochemistric technique. Results: Compared with those in Group B and C, the two DSHX treated groups showed that the liver index, levels of serum HA and ALT and severity of hepatic fibrosis were all significantly lower, the urinary excretion of Hyp was significantly higher; the Col Ⅰ and Col-Ⅲ expression was lower (Col Ⅰ :1. 23±1.14,1. 07±0. 96 vs 4.18±2. 26, 3. 22±1. 44, P<0. 01;Col Ⅲ : 1. 31±0. 69, , 1. 09± 0.58 vs 3.04±0.62,2.23±0.58, P<0.05). At the same time, the expression of caspase-3 in Group E was fewer than Group B and C in hepatocytes (3. 09±0. 65 vs 9. 60±2. 32, 8. 82 ±1. 45, P<0.01),but it was extensively expressed in fibrous septal cells(4.52±0.87 vs 1.69±0.23,2.98±0.36, P<0.01). Conclusion: DSHX capsule shows certain therapeutic effect on hepatic fibrosis in rats, and the mechanism might be related with reducing Col Ⅰ and Col Ⅲ deposition, inhibiting hepatocyte apoptosis and promoting fibrous septal cells (mainly the activated hepatic stellate cells) apoptosis.
基金supported by Italian Ministry of Health [Ricerca Corrente 2018]
文摘Extracellular vesicles(EVs)are heterogeneous nano-sized vesicles of endocytic origin shed into blood and other body fluids such as urine,saliva,seminal fluid,ascites,amniotic liquid,synovial fluid,breast milk and cerebrospinal fluid(CSF)by quite all cell types.EVs actively contribute to intercellular communication as they carry bioactive molecules that are selectively incorporated by the originating cell and are delivered to recipient cells over long and short distances(Simons and Raposo,2009).EVs can be divided into three main groups according to their size and cellular origin:1)exosomes(40–120 nm),that have an endocytic origin and are formed by inward budding of the limiting membrane of multivescicular bodies,which fuse with the plasma membrane and release exosomes into the extracellular space;2)microvesicles(50–1000 nm),budding directly off the plasma membrane;3)apoptotic bodies(>1000 nm),which are released during apoptosis.Besides originating via distinct processes,different subtypes of EVs carry different proteins within their membrane and luminal compartments that reflect the phenotype of the tissue of origin.
