Adolescence represents specific period of life and development,when young person is going through physical,emotional and mental changes,risking poor general and oral health outcome that might affect quality of life.Ne...Adolescence represents specific period of life and development,when young person is going through physical,emotional and mental changes,risking poor general and oral health outcome that might affect quality of life.Negative effects of poor oral health are various:difficulties with speaking and eating,pain and malfunction.This might cause emotional and mental problems,leading to avoidance of speaking,smiling and socializing with friends.Cross-sectional study included 234 participants(15-16 years old).The research instruments used in the study were questionnaire and clinical examination.Questionnaire referred to socio-demographic characteristics of participants,their oral and general health related habits,self-evaluation of confidence and successfulness and effect of oral impacts on daily performances.Clinical examination was used to determine dental status in terms of tooth loss,need for prosthodontic rehabilitation and presence of dental malocclusion.Results:24.6%(58)of adolescents had at least one tooth missing.Significant difference between the genders was obtained,as 32.5%girls had at least one tooth extracted compared to 8.1%of boys.Normative need for prosthodontic rehabilitation was present in 22.0%of subjects.Dental problems negatively affected their sleep and emotions(14.4%and 10.2%few times in period of one month).Data analyses pointed one correlation between extracted teeth and psychosocial aspect of participants'life(difficulties and embarrassment when smiling,talking and spending time with friends,emotional problems).Unmet normative need for prosthodonic or orthodontic treatment affected participants'ability to sleep and relax.Percentage of adolescents with extracted teeth was high,as well as normative treatment need for prosthodontic rehabilitation.Poor dental status affected quality of life of adolescents,especially emotional and mental well-being.Dental service should provide sufficient information to adolescents about prevention of tooth loss and consequences of teeth extraction,not only within masticatory function but also concerning oral health related quality of life.展开更多
Oral potentially malignant disorders(OPMDs)are precursors of oral squamous cell carcinoma(OSCC).Deregulated cellular energy metabolism is a critical hallmark of cancer cells.Peroxisome proliferator-activated receptor-...Oral potentially malignant disorders(OPMDs)are precursors of oral squamous cell carcinoma(OSCC).Deregulated cellular energy metabolism is a critical hallmark of cancer cells.Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1α)plays vital role in mitochondrial energy metabolism.However,the molecular mechanism of PGC1αon OPMDs progression is less unclear.Therefore,we investigated the effects of knockdown PGC1αon human dysplastic oral keratinocytes(DOKs)comprehensively,including cell proliferation,cell cycle,apoptosis,xenograft tumor,mitochondrial DNA(mtDNA),mitochondrial electron transport chain complexes(ETC),reactive oxygen species(ROS),oxygen consumption rate(OCR),extracellular acidification rate(ECAR),and glucose uptake.We found that knockdown PGC1αsignificantly inhibited the proliferation of DOKs in vitro and tumor growth in vivo,induced S-phase arrest,and suppressed PI3K/Akt signaling pathway without affecting cell apoptosis.Mechanistically,downregulated of PGC1αdecreased mtDNA,ETC,and OCR,while enhancing ROS,glucose uptake,ECAR,and glycolysis by regulating lactate dehydrogenase A(LDHA).Moreover,SR18292(an inhibitor of PGC1α)induced oxidative phosphorylation dysfunction of DOKs and declined DOK xenograft tumor progression.Thus,our work suggests that PGC1αplays a crucial role in cell proliferation by reprograming energy metabolism and interfering with energy metabolism,acting as a potential therapeutic target for OPMDs.展开更多
文摘Adolescence represents specific period of life and development,when young person is going through physical,emotional and mental changes,risking poor general and oral health outcome that might affect quality of life.Negative effects of poor oral health are various:difficulties with speaking and eating,pain and malfunction.This might cause emotional and mental problems,leading to avoidance of speaking,smiling and socializing with friends.Cross-sectional study included 234 participants(15-16 years old).The research instruments used in the study were questionnaire and clinical examination.Questionnaire referred to socio-demographic characteristics of participants,their oral and general health related habits,self-evaluation of confidence and successfulness and effect of oral impacts on daily performances.Clinical examination was used to determine dental status in terms of tooth loss,need for prosthodontic rehabilitation and presence of dental malocclusion.Results:24.6%(58)of adolescents had at least one tooth missing.Significant difference between the genders was obtained,as 32.5%girls had at least one tooth extracted compared to 8.1%of boys.Normative need for prosthodontic rehabilitation was present in 22.0%of subjects.Dental problems negatively affected their sleep and emotions(14.4%and 10.2%few times in period of one month).Data analyses pointed one correlation between extracted teeth and psychosocial aspect of participants'life(difficulties and embarrassment when smiling,talking and spending time with friends,emotional problems).Unmet normative need for prosthodonic or orthodontic treatment affected participants'ability to sleep and relax.Percentage of adolescents with extracted teeth was high,as well as normative treatment need for prosthodontic rehabilitation.Poor dental status affected quality of life of adolescents,especially emotional and mental well-being.Dental service should provide sufficient information to adolescents about prevention of tooth loss and consequences of teeth extraction,not only within masticatory function but also concerning oral health related quality of life.
基金supported by the National Natural Science Foundations of China(82372735,82141130).
文摘Oral potentially malignant disorders(OPMDs)are precursors of oral squamous cell carcinoma(OSCC).Deregulated cellular energy metabolism is a critical hallmark of cancer cells.Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1α)plays vital role in mitochondrial energy metabolism.However,the molecular mechanism of PGC1αon OPMDs progression is less unclear.Therefore,we investigated the effects of knockdown PGC1αon human dysplastic oral keratinocytes(DOKs)comprehensively,including cell proliferation,cell cycle,apoptosis,xenograft tumor,mitochondrial DNA(mtDNA),mitochondrial electron transport chain complexes(ETC),reactive oxygen species(ROS),oxygen consumption rate(OCR),extracellular acidification rate(ECAR),and glucose uptake.We found that knockdown PGC1αsignificantly inhibited the proliferation of DOKs in vitro and tumor growth in vivo,induced S-phase arrest,and suppressed PI3K/Akt signaling pathway without affecting cell apoptosis.Mechanistically,downregulated of PGC1αdecreased mtDNA,ETC,and OCR,while enhancing ROS,glucose uptake,ECAR,and glycolysis by regulating lactate dehydrogenase A(LDHA).Moreover,SR18292(an inhibitor of PGC1α)induced oxidative phosphorylation dysfunction of DOKs and declined DOK xenograft tumor progression.Thus,our work suggests that PGC1αplays a crucial role in cell proliferation by reprograming energy metabolism and interfering with energy metabolism,acting as a potential therapeutic target for OPMDs.
