Objective: To investigate the effect of recombinant human osteoprotegerin combined with tinidazole on mice with periodontitis and the effect on serum RANKL and MCP-1 levels. Methods: 80 SPF-cleaned mice were randomly ...Objective: To investigate the effect of recombinant human osteoprotegerin combined with tinidazole on mice with periodontitis and the effect on serum RANKL and MCP-1 levels. Methods: 80 SPF-cleaned mice were randomly divided into 4 groups, 20 each, model group, tinidazole group and recombinant human osteoprotegerin group were modeled by Kimura et al., and tinidazole group received tinidazole. After intragastric administration, the recombinant human osteoprotegerin group was injected with recombinant human osteoprotegerin in the periodontal pocket according to the tinidazole group. The periodontal changes of the four groups of mice were observed and recorded, and the gingival rating was performed. Epithelial tissue morphology was observed by hematoxylin-eosin (HE) staining. Serum levels of IL-4, IL-6, RANKL and MCP-1 were measured by enzyme-linked immunosorbent assay. Results:After the intervention, the model group developed severe inflammatory reactions, including redness, hemorrhage, and deep periodontal pockets. The teeth were significantly loosened. The mice in the tinidazole group and the recombinant human osteoprotegerin group recovered substantially, and the gingival rating of the recombinant human osteoprotegerin group was better than that. The tinidazole group and the model group (P<0.05). The results of HE staining showed that the model group had edema, vasodilation and a large amount of inflammatory infiltration. The epithelial structure of the mice in the tinidazole group and the recombinant human osteoprotegerin group was intact and arranged closely and orderly. After intervention, the IL-4 in the tinidazole group and the recombinant human osteoprotegerin group was significantly higher than the model group and IL-6 was significantly lower than the model group (P<0.05), and the recombinant human osteoprotegerin group IL-4 was significantly higher after the intervention. IL-6 was significantly lower in the tinidazole group than in the tinidazole group (P<0.05). After the intervention, the tinidazole group and the recombinant human osteoprotegerin group were significantly reduced, and the recombinant human osteoprotegerin group RAKNL and MCP-1 were significantly lower than the model group (P>0.05). Conclusion: Recombinant human osteoprotegerin combined with tinidazole has a better therapeutic effect on gums and teeth in mice with periodontitis, and can lower the levels of RAKNL and MCP-1 in serum, inhibit bone resorption and protect teeth.展开更多
Objective: To observe effects of ninadab on NF-κB expression in lung tissue and PCⅢlevel in bronchoalveolar lavage fluid induced by lipopolysaccharide-induced ARDS in rats. Methods: Forty healthy SD rats were random...Objective: To observe effects of ninadab on NF-κB expression in lung tissue and PCⅢlevel in bronchoalveolar lavage fluid induced by lipopolysaccharide-induced ARDS in rats. Methods: Forty healthy SD rats were randomly divided into control group, group A, group B and group C, with 10 in each group. Rats in group A, group B and group C were successful in modeling. In control group, rats in group A were injected with saline only. Rats in group B were treated with nisinib at 7 days and rats in group C were treated with nisinib at 14 days. The expression of NF-κB in lung tissue, the level of β-EP in plasma and the level of PCⅢ in bronchoalveolar perfusion fluid. Results: There were significant differences in the expression of NF-κB positive cells and plasma β-EP among groups. Compared with the control group, the expression of NF-κB positive cells and plasma β-EP in group A, group B and group C were significantly higher than those in control group, Compared with group A, the expression of NF-κB positive cells and plasma β-EP in group B and group C were significantly lower than those in group A. Compared with group B, the expression of NF-κB positive cells and plasma β-EP in group C were significantly higher than those in group B. There was a significant difference between the 4 groups on the 7th, 14th and 28th days. Compared with the control group, PCⅢ levels in the 7th, 14th and 28th days in group A, group B and group C were significantly higher. Compared with group A, group B, group C, the content of PCⅢon the 7th day, the 14th day and the 28th day in group C was significantly higher. Conclusion:Nidanib on lipopolysaccharide-induced ARDS rats have a good protective effect, and its mechanism may be related to the reduction of NF-κB expression in bronchoalveolar perfusion solution PCⅢ level.展开更多
文摘Objective: To investigate the effect of recombinant human osteoprotegerin combined with tinidazole on mice with periodontitis and the effect on serum RANKL and MCP-1 levels. Methods: 80 SPF-cleaned mice were randomly divided into 4 groups, 20 each, model group, tinidazole group and recombinant human osteoprotegerin group were modeled by Kimura et al., and tinidazole group received tinidazole. After intragastric administration, the recombinant human osteoprotegerin group was injected with recombinant human osteoprotegerin in the periodontal pocket according to the tinidazole group. The periodontal changes of the four groups of mice were observed and recorded, and the gingival rating was performed. Epithelial tissue morphology was observed by hematoxylin-eosin (HE) staining. Serum levels of IL-4, IL-6, RANKL and MCP-1 were measured by enzyme-linked immunosorbent assay. Results:After the intervention, the model group developed severe inflammatory reactions, including redness, hemorrhage, and deep periodontal pockets. The teeth were significantly loosened. The mice in the tinidazole group and the recombinant human osteoprotegerin group recovered substantially, and the gingival rating of the recombinant human osteoprotegerin group was better than that. The tinidazole group and the model group (P<0.05). The results of HE staining showed that the model group had edema, vasodilation and a large amount of inflammatory infiltration. The epithelial structure of the mice in the tinidazole group and the recombinant human osteoprotegerin group was intact and arranged closely and orderly. After intervention, the IL-4 in the tinidazole group and the recombinant human osteoprotegerin group was significantly higher than the model group and IL-6 was significantly lower than the model group (P<0.05), and the recombinant human osteoprotegerin group IL-4 was significantly higher after the intervention. IL-6 was significantly lower in the tinidazole group than in the tinidazole group (P<0.05). After the intervention, the tinidazole group and the recombinant human osteoprotegerin group were significantly reduced, and the recombinant human osteoprotegerin group RAKNL and MCP-1 were significantly lower than the model group (P>0.05). Conclusion: Recombinant human osteoprotegerin combined with tinidazole has a better therapeutic effect on gums and teeth in mice with periodontitis, and can lower the levels of RAKNL and MCP-1 in serum, inhibit bone resorption and protect teeth.
文摘Objective: To observe effects of ninadab on NF-κB expression in lung tissue and PCⅢlevel in bronchoalveolar lavage fluid induced by lipopolysaccharide-induced ARDS in rats. Methods: Forty healthy SD rats were randomly divided into control group, group A, group B and group C, with 10 in each group. Rats in group A, group B and group C were successful in modeling. In control group, rats in group A were injected with saline only. Rats in group B were treated with nisinib at 7 days and rats in group C were treated with nisinib at 14 days. The expression of NF-κB in lung tissue, the level of β-EP in plasma and the level of PCⅢ in bronchoalveolar perfusion fluid. Results: There were significant differences in the expression of NF-κB positive cells and plasma β-EP among groups. Compared with the control group, the expression of NF-κB positive cells and plasma β-EP in group A, group B and group C were significantly higher than those in control group, Compared with group A, the expression of NF-κB positive cells and plasma β-EP in group B and group C were significantly lower than those in group A. Compared with group B, the expression of NF-κB positive cells and plasma β-EP in group C were significantly higher than those in group B. There was a significant difference between the 4 groups on the 7th, 14th and 28th days. Compared with the control group, PCⅢ levels in the 7th, 14th and 28th days in group A, group B and group C were significantly higher. Compared with group A, group B, group C, the content of PCⅢon the 7th day, the 14th day and the 28th day in group C was significantly higher. Conclusion:Nidanib on lipopolysaccharide-induced ARDS rats have a good protective effect, and its mechanism may be related to the reduction of NF-κB expression in bronchoalveolar perfusion solution PCⅢ level.
