A new design of a bioelectric amplifier that has better parameters than conventional designs is presented. The design allows the construction of bioelectric amplifier with improved parameters in terms of common-mode r...A new design of a bioelectric amplifier that has better parameters than conventional designs is presented. The design allows the construction of bioelectric amplifier with improved parameters in terms of common-mode rejection ratio and phase and magnitude errors. The voltage gain is easily adapted to a wide range of biomedical applications. The experimental and simulation results of the designed bioelectric amplifier are also included.展开更多
In this paper,a dynamic linear detecting method,that the non-linear coefficient NL% was led and the non-linearity of data were estimated continuously and dynamically and determined when NL% exceeded reference value (...In this paper,a dynamic linear detecting method,that the non-linear coefficient NL% was led and the non-linearity of data were estimated continuously and dynamically and determined when NL% exceeded reference value (5%),was used for data processing and could solve the problem caused by the phenomenon of substrate depleting occurred following the redox reaction in portable blood sugar analyzer.By contrast to the conventional end-point method,the dynamic linear detecting method is based on multipoint data collecting.Experiments of measuring the calibration glucose solution with 8 various concentrations from 50 mg/dl to 400 mg/dl were carried out with the analyzer developed by our group.The linear regression curve,whose correlation for the data was 0.9995 and the residual was 2.8080,were obtained.The obtained correlation,residual, and the computation workload are all fit for the portable blood sugar analyzer.展开更多
Secretome, the totality of secreted proteins, is viewed as a promising pool of candidate cancer biomarkers. Simple and reliable methods for identifying secreted proteins are highly desired. We used an optimized semi-s...Secretome, the totality of secreted proteins, is viewed as a promising pool of candidate cancer biomarkers. Simple and reliable methods for identifying secreted proteins are highly desired. We used an optimized semi-shotgun liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) method to analyze the secretome of breast cancer cell line MDA-MB-231. A total of 464 proteins were identified. About 63% of the proteins were classified as secreted proteins, including many promising breast cancer biomarkers, which were thought to be correlated with tumorigenesis, tumor development and metastasis. These results suggest that the optimized method may be a powerful strategy for cell line secretome profiling, and can be used to find potential cancer biomarkers with great clinical significance.展开更多
Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time...Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological specimens.Traditional efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout rates.In this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate algorithms.Here,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector sensitivity.By using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm resolution.PAR-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure times.Notably,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 Hz.We posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.展开更多
This talk will discuss single-molecule detection that shows on dual-color optical imaging data, image processing and statistical analysis can reliably differentiate random
文摘A new design of a bioelectric amplifier that has better parameters than conventional designs is presented. The design allows the construction of bioelectric amplifier with improved parameters in terms of common-mode rejection ratio and phase and magnitude errors. The voltage gain is easily adapted to a wide range of biomedical applications. The experimental and simulation results of the designed bioelectric amplifier are also included.
文摘In this paper,a dynamic linear detecting method,that the non-linear coefficient NL% was led and the non-linearity of data were estimated continuously and dynamically and determined when NL% exceeded reference value (5%),was used for data processing and could solve the problem caused by the phenomenon of substrate depleting occurred following the redox reaction in portable blood sugar analyzer.By contrast to the conventional end-point method,the dynamic linear detecting method is based on multipoint data collecting.Experiments of measuring the calibration glucose solution with 8 various concentrations from 50 mg/dl to 400 mg/dl were carried out with the analyzer developed by our group.The linear regression curve,whose correlation for the data was 0.9995 and the residual was 2.8080,were obtained.The obtained correlation,residual, and the computation workload are all fit for the portable blood sugar analyzer.
基金Supported by National Natural Science Foundation of China (No.50725622, No. 30670495)Ministry of Science and Technology of China (No. 2006CB910100)Special Funding from Shanghai Center for Systems Biomedicine
文摘Secretome, the totality of secreted proteins, is viewed as a promising pool of candidate cancer biomarkers. Simple and reliable methods for identifying secreted proteins are highly desired. We used an optimized semi-shotgun liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) method to analyze the secretome of breast cancer cell line MDA-MB-231. A total of 464 proteins were identified. About 63% of the proteins were classified as secreted proteins, including many promising breast cancer biomarkers, which were thought to be correlated with tumorigenesis, tumor development and metastasis. These results suggest that the optimized method may be a powerful strategy for cell line secretome profiling, and can be used to find potential cancer biomarkers with great clinical significance.
基金supported by the National Key R&D Program of China (2022YFC3401100)the National Natural Science Foundation of China (62025501,92150301,62335008).
文摘Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological specimens.Traditional efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout rates.In this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate algorithms.Here,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector sensitivity.By using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm resolution.PAR-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure times.Notably,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 Hz.We posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.
文摘This talk will discuss single-molecule detection that shows on dual-color optical imaging data, image processing and statistical analysis can reliably differentiate random
