The sinking of phytoplankton is critical to organic matter transportation in the ocean and it is an essential process for the formation of coastal hypoxic zones.This study was based on a field investigation conducted ...The sinking of phytoplankton is critical to organic matter transportation in the ocean and it is an essential process for the formation of coastal hypoxic zones.This study was based on a field investigation conducted during the summer of 2022 in the Changjiang River(Yangtze River) Estuary(CJE) and its adjacent waters.The settling column method was employed to measure the sinking velocity(SV) of different size fractions of phytoplankton at the surface of the sea and to analyze their environmental control mechanisms.The findings reveal significant spatial variation in phytoplankton SV(-0.55-2.41 m/d) within the CJE.High-speed sinking was predominantly observed in phosphate-depleted regions beyond the CJE front.At the same time,an upward trend was more commonly observed in the phosphate-rich regions near the CJE mouth.The SV ranges for different sizefractionated phytoplankton,including micro-(>20 μm),nano-(2-20 μm),and picophytoplankton(0.7-2 μm),were-0.50-4.74 m/d,-1.04-1.59 m/d,and-1.24-1.65 m/d,respectively.Correlation analysis revealed a significant negative correlation between SV and dissolved inorganic phosphorus(DIP),implying that the influence of DIP contributes to SV.The variations in phytoplankton alkaline phosphatase activity suggested a significant increase in SV across all size fractions in the event of phosphorus limitation.Phytoplankton communities with limited photo synthetic capacity(maximum photochemical efficience,Fv/Fm <0.3) were found to have higher SV than that of communities with strong capacity,suggesting a link between sinking and alterations in physiological conditions due to phosphate depletion.The findings from the in situ phosphate enrichment experiments confirmed a marked decrease in SV following phosphate supplementation.These findings suggest that phosphorus limitation is the primary driver of elevated SV in the CJE.This study enhances the comprehension of the potential mechanisms underlying hypoxic zone formation in the CJE,providing novel insights into how nearshore eutrophication influences organic carbon migration.展开更多
Generalized Additive Models(GAMs)are widely employed in ecological research,serving as a powerful tool for ecologists to explore complex nonlinear relationships between a response variable and predictors.Nevertheless,...Generalized Additive Models(GAMs)are widely employed in ecological research,serving as a powerful tool for ecologists to explore complex nonlinear relationships between a response variable and predictors.Nevertheless,evaluating the relative importance of predictors with concurvity(analogous to collinearity)on response variables in GAMs remains a challenge.To address this challenge,we developed an R package named gam.hp.gam.hp calculates individual R^(2) values for predictors,based on the concept of'average shared variance',a method previously introduced for multiple regression and canonical analyses.Through these individual R^(2)s,which add up to the overall R^(2),researchers can evaluate the relative importance of each predictor within GAMs.We illustrate the utility of the gam.hp package by evaluating the relative importance of emission sources and meteorological factors in explaining ozone concentration variability in air quality data from London,UK.We believe that the gam.hp package will improve the interpretation of results obtained from GAMs.展开更多
The gut microbiota significantly influences host physiology and provides essential ecosystem services.While diet can affect the composition of the gut microbiota,the gut microbiota can also help the host adapt to spec...The gut microbiota significantly influences host physiology and provides essential ecosystem services.While diet can affect the composition of the gut microbiota,the gut microbiota can also help the host adapt to specific dietary habits.The carrion crow(Corvus corone),an urban facultative scavenger bird,hosts an abundance of pathogens due to its scavenging behavior.Despite this,carrion crows infrequently exhibit illness,a phenomenon related to their unique physiological adaptability.At present,however,the role of the gut microbiota remains incompletely understood.In this study,we performed a comparative analysis using 16S rRNA amplicon sequencing technology to assess colonic content in carrion crows and 16 other bird species with different diets in Beijing,China.Our findings revealed that the dominant gut microbiota in carrion crows was primarily composed of Proteobacteria(75.51%)and Firmicutes(22.37%).Significant differences were observed in the relative abundance of Enterococcus faecalis among groups,highlighting its potential as a biomarker of facultative scavenging behavior in carrion crows.Subsequently,E.faecalis isolated from carrion crows was transplanted into model mice to explore the protective effects of this bacterial community against Salmonella enterica infection.Results showed that E.faecalis down-regulated the expression of pro-inflammatory cytokines tumor necrosis factor alpha(TNF-α),interferon gamma(IFN-γ),and interleukin 6(IL-6),prevented S.enterica colonization,and regulated the composition of gut microbiota in mice,thereby modulating the host’s immune regulatory capacity.Therefore,E.faecalis exerts immunoregulatory and anti-pathogenic functions in carrion crows engaged in scavenging behavior,offering a representative case of how the gut microbiota contributes to the protection of hosts with specialized diets.展开更多
In recent decades,the exploration and development of marine oil and gas resources have increased significantly to meet the increasing energy demand of mankind.The Bohai Sea is a semi-closed continental sea that has a ...In recent decades,the exploration and development of marine oil and gas resources have increased significantly to meet the increasing energy demand of mankind.The Bohai Sea is a semi-closed continental sea that has a weak water exchange capacity and high ecological fragility.However,at present,more than 200 oil platforms have been built in the Bohai Sea,with more than 270 offshore oil pipelines having a length exceeding 1600 km.The oil spill pollution of offshore platforms has a great impact on the marine environment and ecosystems.Therefore,a comprehensive assessment of its risks is of great practical significance.This paper systematically constructs a comprehensive oil spill risk assessment model that combines the oil spill risk probability model and the ocean hydrodynamic model.This paper uses the Bohai Sea offshore pipeline as an example to assess its oil spill risk.The high-risk-value areas of the Bohai Sea offshore pipeline are mainly distributed at the bottom of Liaodong Bay,the bottom of Bohai Bay,near the Caofeidian area,and the northern part of the Yellow River Estuary.展开更多
Consideration of stable isotopes in precipitation is valuable for investigating hydrological processes.Therefore,correcting the measured isotopic composition of precipitation under below-cloud evaporation is necessary...Consideration of stable isotopes in precipitation is valuable for investigating hydrological processes.Therefore,correcting the measured isotopic composition of precipitation under below-cloud evaporation is necessary.An accurate description of the underlying processes affecting stable isotopic composition of precipitation could help improve our understanding of the water cycle.The transitivity between monsoonal and arid climates was reflected by the evaporation rate of falling raindrops in precipitation in the Qilian Mountains,a typical transition zone between Tibetan Plateau and arid region of China.Considering 1310 precipitation event-scale samples,based on stable isotope analysis method,the mean below-cloud evaporation rate(f)in the study area was measured as 12.00%during the summer half-year(May-October).The evaporation rate on the northern slopes(12.70%)of the Qilian Mountains in China was significantly higher than that on the southern slopes(9.98%).The transition between monsoonal and arid climates was reflected in the evaporation rate of falling raindrops during precipitation in the Qilian Mountains of China.Below-cloud evaporation contributed to a noticeable enrichment of stable isotopes in the precipitation in the study area.The monthly precipitationδ^(18)O enrichment rate in the Qilian Mountains of China from May to October was 29.18%,23.35%,25.60%,22.99%,31.64%,and 14.72%,respectively.For every 1.00%increase in the evaporation rate of raindrops in Qilian Mountains of China,the changes in the concentration of oxygen isotopes from the bottom of the clouds to the ground increased by 0.92‰;however,with an evaporation rate of<5.00%,for every 1.00%increase in the evaporation rate of raindrops the changes in the concentration of oxygen isotopes from the bottom of the clouds to the ground increased by 1.00‰could also be observed.Furthermore,altitude was an important factor affecting below-cloud evaporation in the study area.展开更多
Brine shrimp Artemia franciscana provide food for many migrating and staging birds that spend summer and fall on Great Salt Lake,Utah,USA.Artemia produce live young and cysts(hard-walled eggs);these cysts are commerci...Brine shrimp Artemia franciscana provide food for many migrating and staging birds that spend summer and fall on Great Salt Lake,Utah,USA.Artemia produce live young and cysts(hard-walled eggs);these cysts are commercially harvested on Great Salt Lake and support a large industry in Utah.It is unclear the impact that millions of hungry birds have on the Artemia population in the lake.To help assess that,this study evaluated cyst viability(percentage of cysts that contain an embryo)and hatchability(percent of cysts that hatch)from cysts that had passed through the digestive tract of eared grebes Podiceps nigricollis and cysts obtained directly from Great Salt Lake at the same site where each grebe was collected.Hatchability was significantly higher for cysts collected from the water column(19%)than from the stomach(0.3%)or intestines(3%)of eared grebes.Viability also was significantly different for cysts collected from the water column(29%),stomach(0.7%),and intestines(5%).These results indicate that eared grebes nutritionally benefit from eating cysts and that they may be an important food source for grebes in late fall after the adult population of Artemia dies off due to the water becoming too cold.Also,enough cysts survive their passage through the digestive system that grebes can vector hatchable cysts to other waterbodies.展开更多
Green tides caused by the bloom of Ulva prolifera have affected the Yellow Sea annually since 2007.To explore the intrinsic causes of successive blooms,field investigations and indoor simulation experiments were condu...Green tides caused by the bloom of Ulva prolifera have affected the Yellow Sea annually since 2007.To explore the intrinsic causes of successive blooms,field investigations and indoor simulation experiments were conducted using micro-propagules of U.prolifera.From January to February 2014,micro-propagules of various green algae,including U.prolifera,Ulva flexuosa,and Ulva linza,were prevalent in the coastal waters of Rudong,Lianyungang,and Qingdao.However,in March 2014,micro-propagules of U.prolifera were not detected in the coastal waters of Lianyungang and Qingdao.Neither the spores nor gametes of U.prolifera can germinate at temperatures below 5℃.At 2℃and 5℃,U.prolifera spores and gametes could survive for 1-3 months at a light intensity range of 0-30μmol m^(-2)s^(-1)and for less than 1 month if the light intensity exceeded 30μmol m^(-2)s^(-1).By integrating indoor experiments and field investigations,this study found that U.prolifera spores and gametes could overwinter in the Rudong coastal area,which is an important factor for the annual emergence of recurring green tides in the Yellow Sea.展开更多
Since 2015, green tides have been blooming in offshore waters of Qinhuangdao, with serious impacts on the local ecological environment and tourism. Ulva australis, Bryopsis plumosa, and U. prolifera are the dominant s...Since 2015, green tides have been blooming in offshore waters of Qinhuangdao, with serious impacts on the local ecological environment and tourism. Ulva australis, Bryopsis plumosa, and U. prolifera are the dominant species of Qinhuangdao green tides, following a sequential succession pattern. Ulva prolifera is the dominant species,with the highest biomass and the greatest influence on the local ecological environment. To study the reason of green tide dominant species succession and U. profilera became the dominant species with the largest biomass,we compared and analyzed the growth and nutrient uptake capacity of the three algae. The results showed that temperature significantly affects the growth of the three species. Within the temperature range of the experimental setup, the optimum temperature for the growth of U. australis, B. plumosa and U. profilera is10℃, 15℃, and 20–25℃, respectively. Combined with the temperature variation trend during green tide bloom development, we believe that temperature is the key environmental factor for the succession of the dominant species. Ulva prolifera has a higher growth rate than U. australis and B. plumosa under the same nitrate,ammonium, and phosphate levels. Significant differences in the maximum absorption rate(R_(max)) and R_(max)/Ks(the relationship between uptake rate and substrate concentration) values indicated that U. prolifera had an apparent competitive advantage over U. australis and B. plumosa regarding nutrient uptake. Therefore, the strong growth and nutrient uptake capacities of U. prolifera might be the main reason for becoming the dominant species with the largest biomass in Qinhuangdao green tides.展开更多
The regulating ways of different water masses affecting the locations and intensities of hypoxia zones were studied based on the time-space continuum data from August 2011 to 2013–2017.The 6-year distribution of the ...The regulating ways of different water masses affecting the locations and intensities of hypoxia zones were studied based on the time-space continuum data from August 2011 to 2013–2017.The 6-year distribution of the hypoxic area in the Changjiang Estuary(CE)and its adjacent waters show that the hypoxic area can be divided into two segments.The southern segment is out of the south branch of the CE,whereas the northern segment is in the junction zone between the South Yellow Sea and the CE.The two segments were divided along the 31.5°–32°N latitude line.The northern and southern segments were dominated by the East China Sea shelf water(ECSSW)and Kuroshio subsurface water(KSW),respectively.When the KSW(salinity>34)intrusion reached the east of 123°E and south of 31°N,hypoxia zones mainly occurred in the southern segment covered by the Changjiang Diluted Water(CDW),meanwhile the Yellow Sea cold water mass may emerge in the northeastern area.When the KSW intensely invaded westward to the region between 122°and 122.5°E and northward to 31.5°N or further north,hypoxia zones appeared in the northern segment.The strength of the KSW with low dissolved oxygen concentration is the basic driving factor for the hypoxia occurrence in the CE.Moreover,the stratification is crucial for the southern segment,whereas the organic matter decomposition is dominated for the northern segment,even with severe hypoxia across the sea surface in the study area.展开更多
Phosphorus(P)is an essential nutrient for many organisms in the ocean,which plays a central role in the stability of ecosystems and the evolution of the environment.The distribution,occurrence and source-sink process ...Phosphorus(P)is an essential nutrient for many organisms in the ocean,which plays a central role in the stability of ecosystems and the evolution of the environment.The distribution,occurrence and source-sink process of P in offshore waters are highly influenced by mariculture activities.P transformation in water-sediment system is the key process in P cycling,however,the mechanism is poorly documented in the coastal sea which is influenced by human activities.Based on the comprehensive surveys in the adjacent waters outside Rushan Bay in May,July and August 2014 and February 2015,the form and transformation of P in the suspended particulate matter(SPM)and surface sediment were analyzed.The results showed that contents of total P,authigenic P and organic P of SPM increased with the increase in distance from the shoreline off Rushan Bay,and the detrital-P decreased.The partition coefficient of P between water and SPM was related to the chemical activity of different forms of P,and a higher reactivity of inorganic P associated with SPM was observed.Hypoxia induced by mariculture changes the distribution and morphological composition of P in SPM and sediment in this typical aquaculture area,which can result in a conversion of sink to source of P in the sediment,thereby having a significant impact on the regional nutrient budget and associated with eutrophication.展开更多
The dry and windy climate and low ground cover in spring in the black soil region of Northeast China make the soil strongly affected by wind erosion,which seriously threatens the food security and ecological security ...The dry and windy climate and low ground cover in spring in the black soil region of Northeast China make the soil strongly affected by wind erosion,which seriously threatens the food security and ecological security of this region.In this paper,based on the daily observation data of 124 meteorological stations in study area from 1961 to 2020,seasonal and monthly wind erosion climate factor(C)in spring(March to May)were calculated by using the method proposed by the Food and Agriculture Organization of the United Nations(FAO),the wind erosion characterization in spring were systematically analyzed based on C by various statistical analysis methods.The results showed that in the past 60 years,spring wind erosion climate factor(CSp)and monthly C of the whole region and each province(region)all showed highly significant decreasing trend,but they began to show rebounded trend in the middle or late 2000s.CSp of the study area showed a significant upward trend since 2008 with an increase of 4.59(10a)^(-1).The main contributors to this upward trend are the changes of C in March and in April.For the four provinces(regions),CSp in Heilongjiang,Jilin,Liaoning and eastern Inner Mongolia all showed rebounded since 2008,2011,2008 and 2009,respectively.The rebounded trend of CSp in eastern Inner Mongolia was the most obvious with a tendency rate of 11.27(10a)^(-1),and its mutation occurred after 1984.The rebound trend of CSp in Heilongjiang Province takes the second place,with a trend rate of 4.72(10a)^(-1),but there’s no obvious time mutation characteristics.The spatial characteristics of CSpand monthly C are similar,showing decreasing characteristics centered on the typical black soil belt of Northeast China.Compared with 1961-1990,in the period from 1991 to 2020,the proportion of high value areas(CSp>35,monthly C>10)has decreased to varying degrees,while the proportion of low value areas(CSp≤10,monthly C≤4)has increased.The trends of seasonal and monthly C in 82.2%~87.7%of the stations show significant decreases at 95%confidence level.CSp is closely related to wind speed at 2m height,temperature difference,minimum temperature and precipitation in the same period,of which the correlation between CSp and wind speed is the strongest,indicating that the main control factor for CSp in the study area is wind speed,but the impact of the change of temperature and precipitation on CSp cannot be ignored.展开更多
The composition and concentration of dissolved free amino acid(DFAA)of seawater samples collected in May 2016 from the surface to the hadal zone of the northern region of the Yap Trench were analyzed by pre-column der...The composition and concentration of dissolved free amino acid(DFAA)of seawater samples collected in May 2016 from the surface to the hadal zone of the northern region of the Yap Trench were analyzed by pre-column derivatization of o-phthalaldehyde.Results show that the average concentration of DFAA in the study area was 0.47±0.36μmol/L.In different sampling stations,the concentrations of DFAA with water depth showed complex variation patterns.At the sediment-seawater interface,the concentrations of DFAA in the western side of the trench were obviously higher than that in its eastern side.In the study area,there were no significant correlations between the concentrations of DFAA and the environmental parameters such as concentrations of chlorophyll a(Chl a),dissolved oxygen(DO),pH,and dissolved inorganic nitrogen(DIN),indicating that the concentrations of DFAA in seawater of the trench are affected by many factors,such as photosynthesis,respiration,temperature,pressure,illumination,and circulation.The dominant DFAA are similar in different water layers of sampling stations,including aspartic acid(Asp),glutamic acid(Glu),glycine(Gly),and serine(Ser).The composition of different amino acids,and the relative abundance of acidic,basic,and neutral amino acids might be related to the sources and consumption of various amino acids.Nine pairs of amino acids in the DFAA showed significantly positive relationship by correlation matrix analysis,suggesting that they might share similar biogeochemical processes.The degradation index(DI)of the DFAA in seawater of the Yap Trench could reflect the degradation,source,and freshness of DFAA in the trench to some extents.This is a preliminary study of amino acids from sea surface to hadal zone in the ocean,more works shall be done in different trenches to reveal their biogeochemical characte ristics in extreme marine environme nts.展开更多
A large number of automatic weather stations with different observation elements and gradient configurations are connected for operation,in order to meet the meteorological service needs of different scenes.The statio...A large number of automatic weather stations with different observation elements and gradient configurations are connected for operation,in order to meet the meteorological service needs of different scenes.The station density and observation frequency are encrypted to obtain observation data with higher spatial and temporal resolution.The original message with fixed element data location is the data combination of all observation elements and the maximum observation gradient of each element,which not only has higher invalid data redundancy,but also restricts the efficiency of data collection and processing,and also increases communication costs.An adaptive coding design method for the original message of automatic weather station is proposed.The embedded software coding algorithm of the weather station collector is optimized according to"plug and output"to realize intelligent networking,intelligent identification of observation elements and gradients,and dynamic flexible output of messages with variable length.The intelligent networking and business application of nearly 4000 automatic weather stations across the province show that the networking data acquisition and processing are efficient and stable.展开更多
Background:Mangrove forests are a significant contributor to the global carbon cycle,and the accurate estimation of their gross primary productivity(GPP)is essential for understanding the carbon budget within blue car...Background:Mangrove forests are a significant contributor to the global carbon cycle,and the accurate estimation of their gross primary productivity(GPP)is essential for understanding the carbon budget within blue carbon ecosystems.Little attention has been given to the investigation of spatiotemporal patterns and ecological variations within mangrove ecosystems,as well as the quantitative analysis of the influence of geo-environmental factors on time-series estimations of mangrove GPP.Methods:This study explored the spatiotemporal dynamics of mangrove GPP from 2000 to 2020 in Gaoqiao Mangrove Reserve,China.A leaf area index(LAI)-based light-use efficiency(LUE)model was combined with Landsat data on Google Earth Engine(GEE)to reveal the variations in mangrove GPP using the Mann-Kendall(MK)test and Theil-Sen median trend.Moreover,the spatiotemporal patterns and ecological variations in mangrove ecosystems across regions were explored using four landscape indicators.Furthermore,the effects of six geo-environmental factors(species distribution,offshore distance,elevation,slope,planar curvature and profile curvature)on GPP were investigated using Geodetector and multi-scale geo-weighted regression(MGWR).Results:The results showed that the mangrove forest in the study area experienced an area loss from 766.26 ha in 2000 to 718.29 ha in 2020,mainly due to the conversion to farming,terrestrial forest and aquaculture zones.Landscape patterns indicated high levels of vegetation aggregation near water bodies and aquaculture zones,and low levels of aggregation but high species diversity and distribution density near building zone.The mean value of mangrove GPP continuously increased from 6.35 g C⋅m^(-2)⋅d^(-1) in 2000 to 8.33 g C⋅m^(-2)⋅d^(-1) in 2020,with 23.21%of areas showing a highly and significantly increasing trend(trend value>0.50).The Geodetector and MGWR analyses showed that species distribution,offshore distance and elevation contributed most to the GPP variations.Conclusions:These results provide guidelines for selecting GPP products,and the combination of Geodetector and MGWR based on multiple geo-environmental factors could quantitatively capture the mode,direction,pathway and intensity of the influencing factors on mangrove GPP variation.The findings provide a foundation for understanding the spatiotemporal dynamics of mangrove GPP at the landscape or regional scale.展开更多
Investigations were carried out on spatial and seasonal composition, distribution and abundance of phytoplankton in Adjin lagoon located in south-eastern of Côte d’Ivoire. Samples were collected at six stations ...Investigations were carried out on spatial and seasonal composition, distribution and abundance of phytoplankton in Adjin lagoon located in south-eastern of Côte d’Ivoire. Samples were collected at six stations during the four seasons in 2013 year. Freshwater inflow from Bété, Djibi and Mé Rivers influenced the variability of nutrients concentration in this lagoon. From a seasonal point of view, the parameters studied are significantly affected by freshwater inputs during the rainy season. This period is characterized by high values of turbidity, suspended solids and nutrients in the water. Overall, 66 taxa from six phyla were recorded. The Chlorophyta had the highest species diversity and Cyanobacteria had the highest relative abundance throughout the year. The temporal distribution of phytoplankton showed that the highest values of density were recorded in the long rainy season and the lowest values in the long dry season. Spatially, the highest abundance (297,927 × 10<sup>3</sup> cells·L<sup>-1</sup>) of phytoplankton was found in station 3 and the lowest (74,222 × 10<sup>3</sup> cells·L<sup>-1</sup>) in the station 5.展开更多
As an important land-ocean interaction process,submarine groundwater discharge(SGD)is composed of multiple dynamical processes at different scales and plays an important role in the study of coastal ocean geochemical ...As an important land-ocean interaction process,submarine groundwater discharge(SGD)is composed of multiple dynamical processes at different scales and plays an important role in the study of coastal ocean geochemical budgets.However,most of the existing studies focus on the quantification of the total groundwater discharge,few studies are about the differentiation and quantification of groundwater discharge processes at different scales(i.e.,short-scale SGD and long-scale SGD).As a world-class river,the Huanghe River is highly turbid and heavily regulated by humans.These natural and anthropogenic factors have a significant impact on groundwater discharge processes in the Huanghe River Estuary(HRE).In this study,the distribution patterns of the natural geochemical tracer radium isotopes(^(224)Ra and^(223)Ra)and other hydrological parameters in the HRE were investigated during four cruises.By solving the mass balance of^(224)Ra and^(223)Ra in the HRE,the long-scale SGD flux was quantified as 0.01−0.19 m/d,and the short-scale SGD flux was 0.03−0.04 m/d.The rate of short-scale SGD remained essentially constant among seasons,while the rate of long-scale SGD varied considerably at different periods and showed a synchronous trend with the variation of river discharge.The results of this study are significant for understanding the SGD dynamics in the HRE and the contribution of SGD to the ocean geochemical budgets.展开更多
Environmentally heterogeneous mountains provide opportunities for rapid diversification and speciation.The family Prunellidae(accentors)is a group of birds comprising primarily mountain specialists that have recently ...Environmentally heterogeneous mountains provide opportunities for rapid diversification and speciation.The family Prunellidae(accentors)is a group of birds comprising primarily mountain specialists that have recently radiated across the Palearctic region.This rapid diversification poses challenges to resolving their phylogeny.Herein we sequenced the complete mitogenomes and estimated the phylogeny using all 12(including 28 individuals)currently recognized species of Prunellidae.We reconstructed the mitochondrial genome phylogeny using 13 protein-coding genes of 12 species and 2 Eurasian Tree Sparrows(Passer montanus).Phylogenetic relationships were estimated using a suite of analyses:maximum likelihood,maximum parsimony and the coalescent-based SVDquartets.Divergence times were estimated by implementing a Bayesian relaxed clock model in BEAST2.Based on the BEAST time-calibrated tree,we implemented an ancestral area reconstruction using RASP v.4.3.Our phylogenies based on the maximum likelihood,maximum parsimony and SVDquartets approaches support a clade of large-sized accentors(subgenus Laiscopus)to be sister to all other accentors with small size(subgenus Prunella).In addition,the trees also support the sister relationship of P.immaculata and P.rubeculoides+P.atrogularis with 100%bootstrap support,but the relationships among the remaining eight species in the Prunella clade are poorly resolved.These species cluster in different positions in the three phylogenetic trees and the nodes are often poorly supported.The five nodes separating the seven species diverged simultaneously within less than half million years(i.e.,between 2.71 and 3.15 million years ago),suggesting that the recent radiation is likely responsible for rampant incomplete lineage sorting and gene tree conflicts.Ancestral area reconstruction indicates a central Palearctic region origin for Prunellidae.Our study highlights that whole mitochondrial genome phylogeny can resolve major lineages within Prunellidae but is not sufficient to fully resolve the relationship among the species in the Prunella clade that almost simultaneously diversify during a short time period.Our results emphasize the challenge to reconstruct reliable phylogenetic relationship in a group of recently radiated species.展开更多
An economic and feasible way to use black soldier fly larvae consists of treating livestock manure to convert it into high value-added protein feed.This study was aimed to investigate the effects of different pretreat...An economic and feasible way to use black soldier fly larvae consists of treating livestock manure to convert it into high value-added protein feed.This study was aimed to investigate the effects of different pretreatments on the physiochemical properties of pig manure and its potential in promoting the growth and nutrient accumulation of black soldier fly larvae(BSFL).Fresh pig manure was pretreated by aerobic fermentation(AE),anaerobic fermentation(AN)and high temperature anaerobic fermentation(HAN)for 2 or 5 d,and then inoculated with BSFL.Results indicate that EC value,DOC,DON,TN,NH_(4)^(+)-N and protein content were the highest in HAN pretreated manure.The individual body weight and protein content of BSFL fed with HAN pretreated manure increased by 20%and 30%,respectively,compared with those fed with AE pretreated manure.In terms of performance,BSFL fed with HAN pretreated manure showed the highest waste reduction,bioconversion rate,waste conversion efficiency and protein conversion efficiency.The conclusion of the present study may be helpful for further research on the conversion of organic waste by BSFL.展开更多
Insects are the most widely distributed and most abundant biological resources on earth,and are as precious a biological resource as plants and microorganisms.With the increasing population pressure and the serious sh...Insects are the most widely distributed and most abundant biological resources on earth,and are as precious a biological resource as plants and microorganisms.With the increasing population pressure and the serious shortage of food resources,insects as an ideal food substitute have gradually been paid more attention by scientists.In addition to being used as food and feed,the application value of insects in waste treatment,medicine and other aspects(such as ornamental and pollination)has also been widely recognized around the world.However,industrialization of insect resources still faces many challenges in terms of acceptability,safety,nutrition and productivity.We argue that insect mass production should be purpose-oriented and targeted cultivation.For example,insects used for food and feeding should be safe,tasty and nutritious.Insects used for waste disposal should maximize their survival rate and their conversion rate of the waste.For insects with medicinal significance,it is necessary to clarify their medicinal active ingredients and related pharmacological mechanism,and to cultivate insect strains with high content of active ingredients by proper induction.A goal-driven development strategy will help to avoid the risk of the insect industry technically and managerially.展开更多
基金The National Programme on Global Change and Air-Sea Interaction (PhaseⅡ)—Hypoxia and Acidification Monitoring and Warning Project in the CE under contract No.GASI-01-CJKthe Science Foundation of Donghai Laboratory under contract No.DH-2022KF0215+3 种基金the Oceanic Interdisciplinary Program of Shanghai Jiao Tong UniversityScientific Research Fund of the Second Institute of Oceanography,MNR under contract No.SL2022ZD207the National Key R&D Program of China under contract No.2021YFC3101702the Long-term Observation and Research Plan in the Changjiang Estuary and Adjacent East China Sea (LORCE)Project under contract No.SZ2001。
文摘The sinking of phytoplankton is critical to organic matter transportation in the ocean and it is an essential process for the formation of coastal hypoxic zones.This study was based on a field investigation conducted during the summer of 2022 in the Changjiang River(Yangtze River) Estuary(CJE) and its adjacent waters.The settling column method was employed to measure the sinking velocity(SV) of different size fractions of phytoplankton at the surface of the sea and to analyze their environmental control mechanisms.The findings reveal significant spatial variation in phytoplankton SV(-0.55-2.41 m/d) within the CJE.High-speed sinking was predominantly observed in phosphate-depleted regions beyond the CJE front.At the same time,an upward trend was more commonly observed in the phosphate-rich regions near the CJE mouth.The SV ranges for different sizefractionated phytoplankton,including micro-(>20 μm),nano-(2-20 μm),and picophytoplankton(0.7-2 μm),were-0.50-4.74 m/d,-1.04-1.59 m/d,and-1.24-1.65 m/d,respectively.Correlation analysis revealed a significant negative correlation between SV and dissolved inorganic phosphorus(DIP),implying that the influence of DIP contributes to SV.The variations in phytoplankton alkaline phosphatase activity suggested a significant increase in SV across all size fractions in the event of phosphorus limitation.Phytoplankton communities with limited photo synthetic capacity(maximum photochemical efficience,Fv/Fm <0.3) were found to have higher SV than that of communities with strong capacity,suggesting a link between sinking and alterations in physiological conditions due to phosphate depletion.The findings from the in situ phosphate enrichment experiments confirmed a marked decrease in SV following phosphate supplementation.These findings suggest that phosphorus limitation is the primary driver of elevated SV in the CJE.This study enhances the comprehension of the potential mechanisms underlying hypoxic zone formation in the CJE,providing novel insights into how nearshore eutrophication influences organic carbon migration.
基金supported by the National Natural Science Foundation of China (32271551)National Key Research and Development Program of China (2023YFF0805803)the Metasequoia funding of Nanjing Forestry University。
文摘Generalized Additive Models(GAMs)are widely employed in ecological research,serving as a powerful tool for ecologists to explore complex nonlinear relationships between a response variable and predictors.Nevertheless,evaluating the relative importance of predictors with concurvity(analogous to collinearity)on response variables in GAMs remains a challenge.To address this challenge,we developed an R package named gam.hp.gam.hp calculates individual R^(2) values for predictors,based on the concept of'average shared variance',a method previously introduced for multiple regression and canonical analyses.Through these individual R^(2)s,which add up to the overall R^(2),researchers can evaluate the relative importance of each predictor within GAMs.We illustrate the utility of the gam.hp package by evaluating the relative importance of emission sources and meteorological factors in explaining ozone concentration variability in air quality data from London,UK.We believe that the gam.hp package will improve the interpretation of results obtained from GAMs.
基金supported by the National Key Research and Development Program of China(2022YFC2601602)Major Program of National Natural Science Foundation of China(32090023)Beijing Wildlife Rescue Center,China。
文摘The gut microbiota significantly influences host physiology and provides essential ecosystem services.While diet can affect the composition of the gut microbiota,the gut microbiota can also help the host adapt to specific dietary habits.The carrion crow(Corvus corone),an urban facultative scavenger bird,hosts an abundance of pathogens due to its scavenging behavior.Despite this,carrion crows infrequently exhibit illness,a phenomenon related to their unique physiological adaptability.At present,however,the role of the gut microbiota remains incompletely understood.In this study,we performed a comparative analysis using 16S rRNA amplicon sequencing technology to assess colonic content in carrion crows and 16 other bird species with different diets in Beijing,China.Our findings revealed that the dominant gut microbiota in carrion crows was primarily composed of Proteobacteria(75.51%)and Firmicutes(22.37%).Significant differences were observed in the relative abundance of Enterococcus faecalis among groups,highlighting its potential as a biomarker of facultative scavenging behavior in carrion crows.Subsequently,E.faecalis isolated from carrion crows was transplanted into model mice to explore the protective effects of this bacterial community against Salmonella enterica infection.Results showed that E.faecalis down-regulated the expression of pro-inflammatory cytokines tumor necrosis factor alpha(TNF-α),interferon gamma(IFN-γ),and interleukin 6(IL-6),prevented S.enterica colonization,and regulated the composition of gut microbiota in mice,thereby modulating the host’s immune regulatory capacity.Therefore,E.faecalis exerts immunoregulatory and anti-pathogenic functions in carrion crows engaged in scavenging behavior,offering a representative case of how the gut microbiota contributes to the protection of hosts with specialized diets.
基金supported by the Special Funds for Fundamental Scientific Research Operation of Central Universities(No.202113011)the Guangxi Key Laboratory of Marine Environmental Science,Guangxi Academy of Sciences(No.GXKLHY21-04)+2 种基金the Shandong Provincial Social Science Planning Research Youth Project(No.21DSHJ2)the General Project of National Social Science Fund for Research on the Ideological and Political Courses in Colleges and Universities(No.21VSZ102)the Ministry of Natural Resources Departmental Budget Project‘Research on the Policy and Operation System of the Control System for Land and Space Use’(No.121107000000190014)。
文摘In recent decades,the exploration and development of marine oil and gas resources have increased significantly to meet the increasing energy demand of mankind.The Bohai Sea is a semi-closed continental sea that has a weak water exchange capacity and high ecological fragility.However,at present,more than 200 oil platforms have been built in the Bohai Sea,with more than 270 offshore oil pipelines having a length exceeding 1600 km.The oil spill pollution of offshore platforms has a great impact on the marine environment and ecosystems.Therefore,a comprehensive assessment of its risks is of great practical significance.This paper systematically constructs a comprehensive oil spill risk assessment model that combines the oil spill risk probability model and the ocean hydrodynamic model.This paper uses the Bohai Sea offshore pipeline as an example to assess its oil spill risk.The high-risk-value areas of the Bohai Sea offshore pipeline are mainly distributed at the bottom of Liaodong Bay,the bottom of Bohai Bay,near the Caofeidian area,and the northern part of the Yellow River Estuary.
基金Under the auspices of the Joint Funds of the National Natural Science Foundation of China(No.U22A20592)the National Key Research and Development Program of China(No.2020YFA0607702)+2 种基金the Second Tibetan Plateau Scientific Expedition and Research Program(No.2019QZKK0405)Chinese Academy of Sciences Young Crossover Team Project(No.JCTD-2022-18)Excellent doctoral program in Gansu Province(No.22JR5RA052)。
文摘Consideration of stable isotopes in precipitation is valuable for investigating hydrological processes.Therefore,correcting the measured isotopic composition of precipitation under below-cloud evaporation is necessary.An accurate description of the underlying processes affecting stable isotopic composition of precipitation could help improve our understanding of the water cycle.The transitivity between monsoonal and arid climates was reflected by the evaporation rate of falling raindrops in precipitation in the Qilian Mountains,a typical transition zone between Tibetan Plateau and arid region of China.Considering 1310 precipitation event-scale samples,based on stable isotope analysis method,the mean below-cloud evaporation rate(f)in the study area was measured as 12.00%during the summer half-year(May-October).The evaporation rate on the northern slopes(12.70%)of the Qilian Mountains in China was significantly higher than that on the southern slopes(9.98%).The transition between monsoonal and arid climates was reflected in the evaporation rate of falling raindrops during precipitation in the Qilian Mountains of China.Below-cloud evaporation contributed to a noticeable enrichment of stable isotopes in the precipitation in the study area.The monthly precipitationδ^(18)O enrichment rate in the Qilian Mountains of China from May to October was 29.18%,23.35%,25.60%,22.99%,31.64%,and 14.72%,respectively.For every 1.00%increase in the evaporation rate of raindrops in Qilian Mountains of China,the changes in the concentration of oxygen isotopes from the bottom of the clouds to the ground increased by 0.92‰;however,with an evaporation rate of<5.00%,for every 1.00%increase in the evaporation rate of raindrops the changes in the concentration of oxygen isotopes from the bottom of the clouds to the ground increased by 1.00‰could also be observed.Furthermore,altitude was an important factor affecting below-cloud evaporation in the study area.
基金Supported by the Great Salt Lake Ecosystem Program of Utah Division of Wildlife Resources,Utah Agricultural Experiment Station(article No.9534)and Ecology Center of Utah State Universityapproved by Institutional Animal Care and Use Committee of Utah State University(10087)+2 种基金permitted by the state of Utah(1BAND10069,2COLL10039)the U.S.Bird Banding Lab(21175)and the U.S.Fish and Wildlife Service(MB693916-0).
文摘Brine shrimp Artemia franciscana provide food for many migrating and staging birds that spend summer and fall on Great Salt Lake,Utah,USA.Artemia produce live young and cysts(hard-walled eggs);these cysts are commercially harvested on Great Salt Lake and support a large industry in Utah.It is unclear the impact that millions of hungry birds have on the Artemia population in the lake.To help assess that,this study evaluated cyst viability(percentage of cysts that contain an embryo)and hatchability(percent of cysts that hatch)from cysts that had passed through the digestive tract of eared grebes Podiceps nigricollis and cysts obtained directly from Great Salt Lake at the same site where each grebe was collected.Hatchability was significantly higher for cysts collected from the water column(19%)than from the stomach(0.3%)or intestines(3%)of eared grebes.Viability also was significantly different for cysts collected from the water column(29%),stomach(0.7%),and intestines(5%).These results indicate that eared grebes nutritionally benefit from eating cysts and that they may be an important food source for grebes in late fall after the adult population of Artemia dies off due to the water becoming too cold.Also,enough cysts survive their passage through the digestive system that grebes can vector hatchable cysts to other waterbodies.
基金supported by the Utah Agricultural Experiment StationUtah State University(approved as journal paper Number 9665)+1 种基金UAES Seed Grant,Water Initiative Extension Grant,the U.S.Department of Interior,Bureau of Reclamation[Grant No.R22AP00220]based upon work supported by the U.S.Geological Survey[Grant No.G21AP10623]through the Utah Center for Water Resources Research at the Utah Water Research Laboratory。
基金The work was supported by the Key Laboratory of Ecological Prewarning,Protection and Restoration of Bohai Sea,Ministry of Natural Resources(No.2022107)the Qingdao Postdoctoral Applied Research Project(No.QDBSH202001).
文摘Green tides caused by the bloom of Ulva prolifera have affected the Yellow Sea annually since 2007.To explore the intrinsic causes of successive blooms,field investigations and indoor simulation experiments were conducted using micro-propagules of U.prolifera.From January to February 2014,micro-propagules of various green algae,including U.prolifera,Ulva flexuosa,and Ulva linza,were prevalent in the coastal waters of Rudong,Lianyungang,and Qingdao.However,in March 2014,micro-propagules of U.prolifera were not detected in the coastal waters of Lianyungang and Qingdao.Neither the spores nor gametes of U.prolifera can germinate at temperatures below 5℃.At 2℃and 5℃,U.prolifera spores and gametes could survive for 1-3 months at a light intensity range of 0-30μmol m^(-2)s^(-1)and for less than 1 month if the light intensity exceeded 30μmol m^(-2)s^(-1).By integrating indoor experiments and field investigations,this study found that U.prolifera spores and gametes could overwinter in the Rudong coastal area,which is an important factor for the annual emergence of recurring green tides in the Yellow Sea.
基金The Fund of Key Laboratory of Ecological Prewarning,Protection and Restoration of Bohai Sea,Ministry of Natural Resources under contract No.2022107the Qingdao Postdoctoral Applied Research Project under contract No.QDBSH202001。
文摘Since 2015, green tides have been blooming in offshore waters of Qinhuangdao, with serious impacts on the local ecological environment and tourism. Ulva australis, Bryopsis plumosa, and U. prolifera are the dominant species of Qinhuangdao green tides, following a sequential succession pattern. Ulva prolifera is the dominant species,with the highest biomass and the greatest influence on the local ecological environment. To study the reason of green tide dominant species succession and U. profilera became the dominant species with the largest biomass,we compared and analyzed the growth and nutrient uptake capacity of the three algae. The results showed that temperature significantly affects the growth of the three species. Within the temperature range of the experimental setup, the optimum temperature for the growth of U. australis, B. plumosa and U. profilera is10℃, 15℃, and 20–25℃, respectively. Combined with the temperature variation trend during green tide bloom development, we believe that temperature is the key environmental factor for the succession of the dominant species. Ulva prolifera has a higher growth rate than U. australis and B. plumosa under the same nitrate,ammonium, and phosphate levels. Significant differences in the maximum absorption rate(R_(max)) and R_(max)/Ks(the relationship between uptake rate and substrate concentration) values indicated that U. prolifera had an apparent competitive advantage over U. australis and B. plumosa regarding nutrient uptake. Therefore, the strong growth and nutrient uptake capacities of U. prolifera might be the main reason for becoming the dominant species with the largest biomass in Qinhuangdao green tides.
基金supported by the National Key Research and Development Program of China(No.2021YFC3101702)the Pilot Project of Early Warning Monitoring of Hypoxia and Ocean Acidification in the Changjiang Estuary of the Ministry of Natural Resources of China(MNR)(2020-2022)+2 种基金the Key Laboratory of Marine Ecological Monitoring and Restoration Technologies,MNR(No.ME MRT202009)the Key Laboratory of Marine Ecosystem Dynamics,Second Institute of Oceanography,Ministry of Natural Resources(No.MED202005)the Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station,Ministry of Education&Shanghai Science and Technology Committee(No.ECNU-YDEWS-2020).
文摘The regulating ways of different water masses affecting the locations and intensities of hypoxia zones were studied based on the time-space continuum data from August 2011 to 2013–2017.The 6-year distribution of the hypoxic area in the Changjiang Estuary(CE)and its adjacent waters show that the hypoxic area can be divided into two segments.The southern segment is out of the south branch of the CE,whereas the northern segment is in the junction zone between the South Yellow Sea and the CE.The two segments were divided along the 31.5°–32°N latitude line.The northern and southern segments were dominated by the East China Sea shelf water(ECSSW)and Kuroshio subsurface water(KSW),respectively.When the KSW(salinity>34)intrusion reached the east of 123°E and south of 31°N,hypoxia zones mainly occurred in the southern segment covered by the Changjiang Diluted Water(CDW),meanwhile the Yellow Sea cold water mass may emerge in the northeastern area.When the KSW intensely invaded westward to the region between 122°and 122.5°E and northward to 31.5°N or further north,hypoxia zones appeared in the northern segment.The strength of the KSW with low dissolved oxygen concentration is the basic driving factor for the hypoxia occurrence in the CE.Moreover,the stratification is crucial for the southern segment,whereas the organic matter decomposition is dominated for the northern segment,even with severe hypoxia across the sea surface in the study area.
基金The National Natural Science Foundation of China under contract Nos 41806097,42176048 and 42149902.
文摘Phosphorus(P)is an essential nutrient for many organisms in the ocean,which plays a central role in the stability of ecosystems and the evolution of the environment.The distribution,occurrence and source-sink process of P in offshore waters are highly influenced by mariculture activities.P transformation in water-sediment system is the key process in P cycling,however,the mechanism is poorly documented in the coastal sea which is influenced by human activities.Based on the comprehensive surveys in the adjacent waters outside Rushan Bay in May,July and August 2014 and February 2015,the form and transformation of P in the suspended particulate matter(SPM)and surface sediment were analyzed.The results showed that contents of total P,authigenic P and organic P of SPM increased with the increase in distance from the shoreline off Rushan Bay,and the detrital-P decreased.The partition coefficient of P between water and SPM was related to the chemical activity of different forms of P,and a higher reactivity of inorganic P associated with SPM was observed.Hypoxia induced by mariculture changes the distribution and morphological composition of P in SPM and sediment in this typical aquaculture area,which can result in a conversion of sink to source of P in the sediment,thereby having a significant impact on the regional nutrient budget and associated with eutrophication.
基金supported by the Open Research Fund of Innovation and Open Laboratory of Eco-meteorology in Northeast China,China Meteorological Administration(stqx2019zd02)Heilongjiang Meteorological Science and Technology Research Project(HQGG202004)Heilongjiang Provincial Natural Science Foundation of China(LH2020C105)。
文摘The dry and windy climate and low ground cover in spring in the black soil region of Northeast China make the soil strongly affected by wind erosion,which seriously threatens the food security and ecological security of this region.In this paper,based on the daily observation data of 124 meteorological stations in study area from 1961 to 2020,seasonal and monthly wind erosion climate factor(C)in spring(March to May)were calculated by using the method proposed by the Food and Agriculture Organization of the United Nations(FAO),the wind erosion characterization in spring were systematically analyzed based on C by various statistical analysis methods.The results showed that in the past 60 years,spring wind erosion climate factor(CSp)and monthly C of the whole region and each province(region)all showed highly significant decreasing trend,but they began to show rebounded trend in the middle or late 2000s.CSp of the study area showed a significant upward trend since 2008 with an increase of 4.59(10a)^(-1).The main contributors to this upward trend are the changes of C in March and in April.For the four provinces(regions),CSp in Heilongjiang,Jilin,Liaoning and eastern Inner Mongolia all showed rebounded since 2008,2011,2008 and 2009,respectively.The rebounded trend of CSp in eastern Inner Mongolia was the most obvious with a tendency rate of 11.27(10a)^(-1),and its mutation occurred after 1984.The rebound trend of CSp in Heilongjiang Province takes the second place,with a trend rate of 4.72(10a)^(-1),but there’s no obvious time mutation characteristics.The spatial characteristics of CSpand monthly C are similar,showing decreasing characteristics centered on the typical black soil belt of Northeast China.Compared with 1961-1990,in the period from 1991 to 2020,the proportion of high value areas(CSp>35,monthly C>10)has decreased to varying degrees,while the proportion of low value areas(CSp≤10,monthly C≤4)has increased.The trends of seasonal and monthly C in 82.2%~87.7%of the stations show significant decreases at 95%confidence level.CSp is closely related to wind speed at 2m height,temperature difference,minimum temperature and precipitation in the same period,of which the correlation between CSp and wind speed is the strongest,indicating that the main control factor for CSp in the study area is wind speed,but the impact of the change of temperature and precipitation on CSp cannot be ignored.
基金Supported by the Natural National Science Foundation of China(Nos.42076040,41676067)the National Basic Research Program of China(973 Program)(No.2015CB755904)the 111 Project(No.B13030)。
文摘The composition and concentration of dissolved free amino acid(DFAA)of seawater samples collected in May 2016 from the surface to the hadal zone of the northern region of the Yap Trench were analyzed by pre-column derivatization of o-phthalaldehyde.Results show that the average concentration of DFAA in the study area was 0.47±0.36μmol/L.In different sampling stations,the concentrations of DFAA with water depth showed complex variation patterns.At the sediment-seawater interface,the concentrations of DFAA in the western side of the trench were obviously higher than that in its eastern side.In the study area,there were no significant correlations between the concentrations of DFAA and the environmental parameters such as concentrations of chlorophyll a(Chl a),dissolved oxygen(DO),pH,and dissolved inorganic nitrogen(DIN),indicating that the concentrations of DFAA in seawater of the trench are affected by many factors,such as photosynthesis,respiration,temperature,pressure,illumination,and circulation.The dominant DFAA are similar in different water layers of sampling stations,including aspartic acid(Asp),glutamic acid(Glu),glycine(Gly),and serine(Ser).The composition of different amino acids,and the relative abundance of acidic,basic,and neutral amino acids might be related to the sources and consumption of various amino acids.Nine pairs of amino acids in the DFAA showed significantly positive relationship by correlation matrix analysis,suggesting that they might share similar biogeochemical processes.The degradation index(DI)of the DFAA in seawater of the Yap Trench could reflect the degradation,source,and freshness of DFAA in the trench to some extents.This is a preliminary study of amino acids from sea surface to hadal zone in the ocean,more works shall be done in different trenches to reveal their biogeochemical characte ristics in extreme marine environme nts.
基金Supported by Technical Innovation Team Project of Collaborative Observation and Multi-source Live Data Fusion Analysis of Guangdong Meteorological Bu-reau(GRMCTD202103)R&D Plan Projects of Key Fields in Guangdong Province(2020B1111200001).
文摘A large number of automatic weather stations with different observation elements and gradient configurations are connected for operation,in order to meet the meteorological service needs of different scenes.The station density and observation frequency are encrypted to obtain observation data with higher spatial and temporal resolution.The original message with fixed element data location is the data combination of all observation elements and the maximum observation gradient of each element,which not only has higher invalid data redundancy,but also restricts the efficiency of data collection and processing,and also increases communication costs.An adaptive coding design method for the original message of automatic weather station is proposed.The embedded software coding algorithm of the weather station collector is optimized according to"plug and output"to realize intelligent networking,intelligent identification of observation elements and gradients,and dynamic flexible output of messages with variable length.The intelligent networking and business application of nearly 4000 automatic weather stations across the province show that the networking data acquisition and processing are efficient and stable.
基金This work was supported by Guangdong Basic and Applied Basic Research Foundation(2019A1515010741 and 2021A1515110910)Guangdong Regional Joint Fund-Youth Fund(2020A1515111142)Shenzhen Science and Technology Program(JCYJ20210324093210029).
文摘Background:Mangrove forests are a significant contributor to the global carbon cycle,and the accurate estimation of their gross primary productivity(GPP)is essential for understanding the carbon budget within blue carbon ecosystems.Little attention has been given to the investigation of spatiotemporal patterns and ecological variations within mangrove ecosystems,as well as the quantitative analysis of the influence of geo-environmental factors on time-series estimations of mangrove GPP.Methods:This study explored the spatiotemporal dynamics of mangrove GPP from 2000 to 2020 in Gaoqiao Mangrove Reserve,China.A leaf area index(LAI)-based light-use efficiency(LUE)model was combined with Landsat data on Google Earth Engine(GEE)to reveal the variations in mangrove GPP using the Mann-Kendall(MK)test and Theil-Sen median trend.Moreover,the spatiotemporal patterns and ecological variations in mangrove ecosystems across regions were explored using four landscape indicators.Furthermore,the effects of six geo-environmental factors(species distribution,offshore distance,elevation,slope,planar curvature and profile curvature)on GPP were investigated using Geodetector and multi-scale geo-weighted regression(MGWR).Results:The results showed that the mangrove forest in the study area experienced an area loss from 766.26 ha in 2000 to 718.29 ha in 2020,mainly due to the conversion to farming,terrestrial forest and aquaculture zones.Landscape patterns indicated high levels of vegetation aggregation near water bodies and aquaculture zones,and low levels of aggregation but high species diversity and distribution density near building zone.The mean value of mangrove GPP continuously increased from 6.35 g C⋅m^(-2)⋅d^(-1) in 2000 to 8.33 g C⋅m^(-2)⋅d^(-1) in 2020,with 23.21%of areas showing a highly and significantly increasing trend(trend value>0.50).The Geodetector and MGWR analyses showed that species distribution,offshore distance and elevation contributed most to the GPP variations.Conclusions:These results provide guidelines for selecting GPP products,and the combination of Geodetector and MGWR based on multiple geo-environmental factors could quantitatively capture the mode,direction,pathway and intensity of the influencing factors on mangrove GPP variation.The findings provide a foundation for understanding the spatiotemporal dynamics of mangrove GPP at the landscape or regional scale.
文摘Investigations were carried out on spatial and seasonal composition, distribution and abundance of phytoplankton in Adjin lagoon located in south-eastern of Côte d’Ivoire. Samples were collected at six stations during the four seasons in 2013 year. Freshwater inflow from Bété, Djibi and Mé Rivers influenced the variability of nutrients concentration in this lagoon. From a seasonal point of view, the parameters studied are significantly affected by freshwater inputs during the rainy season. This period is characterized by high values of turbidity, suspended solids and nutrients in the water. Overall, 66 taxa from six phyla were recorded. The Chlorophyta had the highest species diversity and Cyanobacteria had the highest relative abundance throughout the year. The temporal distribution of phytoplankton showed that the highest values of density were recorded in the long rainy season and the lowest values in the long dry season. Spatially, the highest abundance (297,927 × 10<sup>3</sup> cells·L<sup>-1</sup>) of phytoplankton was found in station 3 and the lowest (74,222 × 10<sup>3</sup> cells·L<sup>-1</sup>) in the station 5.
基金The National Natural Science Foundation of China under contract Nos U22A20580,42130410,U2106203 and 41977173the Fundamental Research Funds for the Central Universities,China under contract No.202341002.
文摘As an important land-ocean interaction process,submarine groundwater discharge(SGD)is composed of multiple dynamical processes at different scales and plays an important role in the study of coastal ocean geochemical budgets.However,most of the existing studies focus on the quantification of the total groundwater discharge,few studies are about the differentiation and quantification of groundwater discharge processes at different scales(i.e.,short-scale SGD and long-scale SGD).As a world-class river,the Huanghe River is highly turbid and heavily regulated by humans.These natural and anthropogenic factors have a significant impact on groundwater discharge processes in the Huanghe River Estuary(HRE).In this study,the distribution patterns of the natural geochemical tracer radium isotopes(^(224)Ra and^(223)Ra)and other hydrological parameters in the HRE were investigated during four cruises.By solving the mass balance of^(224)Ra and^(223)Ra in the HRE,the long-scale SGD flux was quantified as 0.01−0.19 m/d,and the short-scale SGD flux was 0.03−0.04 m/d.The rate of short-scale SGD remained essentially constant among seasons,while the rate of long-scale SGD varied considerably at different periods and showed a synchronous trend with the variation of river discharge.The results of this study are significant for understanding the SGD dynamics in the HRE and the contribution of SGD to the ocean geochemical budgets.
基金funded by the National Natural Science Foundation of China(NSFC32020103005)the Third Xinjiang Scientific Expedition and Research(XIKK)(2022xjkk0205)Second Tibetan Plateau Scientific Expedition and Research(2019QZKK0501)。
文摘Environmentally heterogeneous mountains provide opportunities for rapid diversification and speciation.The family Prunellidae(accentors)is a group of birds comprising primarily mountain specialists that have recently radiated across the Palearctic region.This rapid diversification poses challenges to resolving their phylogeny.Herein we sequenced the complete mitogenomes and estimated the phylogeny using all 12(including 28 individuals)currently recognized species of Prunellidae.We reconstructed the mitochondrial genome phylogeny using 13 protein-coding genes of 12 species and 2 Eurasian Tree Sparrows(Passer montanus).Phylogenetic relationships were estimated using a suite of analyses:maximum likelihood,maximum parsimony and the coalescent-based SVDquartets.Divergence times were estimated by implementing a Bayesian relaxed clock model in BEAST2.Based on the BEAST time-calibrated tree,we implemented an ancestral area reconstruction using RASP v.4.3.Our phylogenies based on the maximum likelihood,maximum parsimony and SVDquartets approaches support a clade of large-sized accentors(subgenus Laiscopus)to be sister to all other accentors with small size(subgenus Prunella).In addition,the trees also support the sister relationship of P.immaculata and P.rubeculoides+P.atrogularis with 100%bootstrap support,but the relationships among the remaining eight species in the Prunella clade are poorly resolved.These species cluster in different positions in the three phylogenetic trees and the nodes are often poorly supported.The five nodes separating the seven species diverged simultaneously within less than half million years(i.e.,between 2.71 and 3.15 million years ago),suggesting that the recent radiation is likely responsible for rampant incomplete lineage sorting and gene tree conflicts.Ancestral area reconstruction indicates a central Palearctic region origin for Prunellidae.Our study highlights that whole mitochondrial genome phylogeny can resolve major lineages within Prunellidae but is not sufficient to fully resolve the relationship among the species in the Prunella clade that almost simultaneously diversify during a short time period.Our results emphasize the challenge to reconstruct reliable phylogenetic relationship in a group of recently radiated species.
基金supported by Key R&D Program of Hebei(21327315D,21373804D)the Special Research Assistant Funding Project of Chinese Academy of Sciences(E129ST15)+4 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030302-4)Key Laboratory of Agricultural Water Resources-CAS(ZD201802)Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030302)Youth Innovation Promotion Association CAS(2021095)Hebei Dairy Cattle Innovation Team of Modern Agro-industry Technology Research System(HBCT2018120206).
文摘An economic and feasible way to use black soldier fly larvae consists of treating livestock manure to convert it into high value-added protein feed.This study was aimed to investigate the effects of different pretreatments on the physiochemical properties of pig manure and its potential in promoting the growth and nutrient accumulation of black soldier fly larvae(BSFL).Fresh pig manure was pretreated by aerobic fermentation(AE),anaerobic fermentation(AN)and high temperature anaerobic fermentation(HAN)for 2 or 5 d,and then inoculated with BSFL.Results indicate that EC value,DOC,DON,TN,NH_(4)^(+)-N and protein content were the highest in HAN pretreated manure.The individual body weight and protein content of BSFL fed with HAN pretreated manure increased by 20%and 30%,respectively,compared with those fed with AE pretreated manure.In terms of performance,BSFL fed with HAN pretreated manure showed the highest waste reduction,bioconversion rate,waste conversion efficiency and protein conversion efficiency.The conclusion of the present study may be helpful for further research on the conversion of organic waste by BSFL.
基金supported by Key R&D Program of Hebei(21327315D,21373804D)the Special Research Assistant Funding Project of Chinese Academy of Sciences(E129ST15)+4 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030302-4)Key Laboratory of Agricultural Water Resources-CAS(ZD201802)Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030302)Youth Innovation Promotion Association CAS(2021095)Hebei Dairy Cattle Innovation Team of Modern Agro-industry Technology Research System(HBCT2018120206).
文摘Insects are the most widely distributed and most abundant biological resources on earth,and are as precious a biological resource as plants and microorganisms.With the increasing population pressure and the serious shortage of food resources,insects as an ideal food substitute have gradually been paid more attention by scientists.In addition to being used as food and feed,the application value of insects in waste treatment,medicine and other aspects(such as ornamental and pollination)has also been widely recognized around the world.However,industrialization of insect resources still faces many challenges in terms of acceptability,safety,nutrition and productivity.We argue that insect mass production should be purpose-oriented and targeted cultivation.For example,insects used for food and feeding should be safe,tasty and nutritious.Insects used for waste disposal should maximize their survival rate and their conversion rate of the waste.For insects with medicinal significance,it is necessary to clarify their medicinal active ingredients and related pharmacological mechanism,and to cultivate insect strains with high content of active ingredients by proper induction.A goal-driven development strategy will help to avoid the risk of the insect industry technically and managerially.
