Summary What is already known about this topic?Previous research has primarily examined the issue of hepatitis B vaccine hesitancy in migrant workers and other adult populations.However,there is a lack of studies that...Summary What is already known about this topic?Previous research has primarily examined the issue of hepatitis B vaccine hesitancy in migrant workers and other adult populations.However,there is a lack of studies that have specifically investigated the prevalence of hepatitis B vaccine hesitancy among university students.What is added by this report?展开更多
Summary What is already known about this topic?As one of the populations at high risk of hepatitis B virus(HBV)infection,patients with chronic kidney disease(CKD)require rapid and effective development of hepatitis B ...Summary What is already known about this topic?As one of the populations at high risk of hepatitis B virus(HBV)infection,patients with chronic kidney disease(CKD)require rapid and effective development of hepatitis B surface antibodies(anti-HBs).What is added by this report?The short-course,high-dose regimen of hepatitis B vaccination rapidly induced comparable immunological responses to the routine regimen,achieving a seroconversion rate of 88.5%,a high-response rate of 64.1%,and anti-HBs geometric mean concentrations of 824.1 mIU/mL.What are the implications for public health practice?The short-course,high-dose regimen represents an effective rapid immunization strategy for CKD patients,particularly when administered prior to immunosuppressive therapy.This study provides compelling evidence for optimizing immunization protocols in the CKD population.展开更多
Background:The personality-brain association mechanism has been a topic of interest in the field of neuroscience.Usually,the previous research strategy was to first group the population based on different personality ...Background:The personality-brain association mechanism has been a topic of interest in the field of neuroscience.Usually,the previous research strategy was to first group the population based on different personality traits,and then explore the brain mechanisms corresponding to different personality groups.At present,a“brain-first”research strategy,which uses data-driven approaches instead of personality traits to first group the population,has been adopted to further enhance study objectivity.Methods:Here,we used a data-driven approach following the“brain-first”research strategy to deeply mine the resting-state brain functional magnetic resonance imaging data of 119 healthy participants,classified subjects into different groups based on brain image characteristics,and used the Sixteen Personality Factor Questionnaire to explain the variabilities of resting-state brain characteristics between different groups.Results:We have identified 3 personality–brain connections,including the privateness–left frontoparietal network,liveliness–sensory–motor network,and vigilance–sensory–motor network.Conclusion:We conclude that the above-mentioned three personality factors are based on brain neural activity,independent of the subjective experience of the personality scale creator,and have stronger explanatory power of brain imaging features.展开更多
Background：XB 130 is a recently discovered adaptor protein that is highly expressed in many malignant tumors,but few studies have investigated its role in hepatocellular carcinoma （HCC）.Therefore,this study explore...Background：XB 130 is a recently discovered adaptor protein that is highly expressed in many malignant tumors,but few studies have investigated its role in hepatocellular carcinoma （HCC）.Therefore,this study explored the relationship between this protein and liver cancer and investigated its molecular mechanism of action.Methods：The expression of XB 130 between HCC tissues and adjacent nontumor tissues was compared by real-time polymerase chain reaction,immunochemistry,and Western blotting.XB130 silencing was performed using small hairpin RNA.The effect of silencing XB130 was examined using Cell Counting Kit-8,colony assay,wound healing assay,and cell cycle analysis.Results：We found that XB 130 was highly expressed in HCC tissues （cancer tissues vs.adjacent tissues：0.23 ± 0.02 vs.0.17 ± 0.02,P 〈 0.05） and liver cancer cell lines,particularly MHCC97H and HepG2 （MHCC97H and HepG2 vs.normal liver cell line LO-2：2.35 ± 0.26 and 2.04 ± 0.04 vs.1.00 ± 0.04,respectively,all P 〈 0.05）.The Cell Counting Kit-8 assay,colony formation assay,and xenograft model in nude mice showed that silencing XB130 inhibited cell proliferative ability both in vivo and in vitro,with flow cytometry demonstrating that the cells were arrested in the G0/G1 phase in HepG2 （HepG2 XB130-silenced group [shA] vs.HepG2 scramble group [NA]：74.32 ± 5.86% vs.60.21 ± 3.07%,P 〈 0.05） and that the number of G2/M phase cells was decreased （HepG2 shA vs.HepG2 NA：8.06 ± 2.41% vs.18.36 ± 4.42%,P 〈 0.05）.Furthermore,the cell invasion and migration abilities were impaired,and the levels of the epithelial-mesenchymal transition-related indicators vimentin and N-cadherin were decreased,although the level of E-cadherin was increased after silencingXB130.Western blotting showed that the levels of phosphorylated phosphoinositide 3-kinase （PI3K） and phospho-protein kinase B （p-Akt） also increased,although the level of phosphorylated phosphatase and tensin homolog increased,indicating that XB 130 activated the PI3K/ Akt pathway.Furthermore,we found that a reduction in XB130 increased liver cancer cell sensitivity to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis.Conclusions：Our findings suggest that XB130 might be used as a predictor of liver cancer as well as one of the targets for its treatment.展开更多
文摘Summary What is already known about this topic?Previous research has primarily examined the issue of hepatitis B vaccine hesitancy in migrant workers and other adult populations.However,there is a lack of studies that have specifically investigated the prevalence of hepatitis B vaccine hesitancy among university students.What is added by this report?
基金Supported by the National Science and Technology Major Project of the Ministry of Science and Technology of the People’s Republic of China under Grant[2018ZX10721202]the Key Research and Development Program of Lvliang under Grant[2020SHFZ52]the Key Research and Development Program of Science and Technology of Linfen under Grant[2136].
文摘Summary What is already known about this topic?As one of the populations at high risk of hepatitis B virus(HBV)infection,patients with chronic kidney disease(CKD)require rapid and effective development of hepatitis B surface antibodies(anti-HBs).What is added by this report?The short-course,high-dose regimen of hepatitis B vaccination rapidly induced comparable immunological responses to the routine regimen,achieving a seroconversion rate of 88.5%,a high-response rate of 64.1%,and anti-HBs geometric mean concentrations of 824.1 mIU/mL.What are the implications for public health practice?The short-course,high-dose regimen represents an effective rapid immunization strategy for CKD patients,particularly when administered prior to immunosuppressive therapy.This study provides compelling evidence for optimizing immunization protocols in the CKD population.
基金supported by the National Natural Science Foundation of China(Grant No.82101610)。
文摘Background:The personality-brain association mechanism has been a topic of interest in the field of neuroscience.Usually,the previous research strategy was to first group the population based on different personality traits,and then explore the brain mechanisms corresponding to different personality groups.At present,a“brain-first”research strategy,which uses data-driven approaches instead of personality traits to first group the population,has been adopted to further enhance study objectivity.Methods:Here,we used a data-driven approach following the“brain-first”research strategy to deeply mine the resting-state brain functional magnetic resonance imaging data of 119 healthy participants,classified subjects into different groups based on brain image characteristics,and used the Sixteen Personality Factor Questionnaire to explain the variabilities of resting-state brain characteristics between different groups.Results:We have identified 3 personality–brain connections,including the privateness–left frontoparietal network,liveliness–sensory–motor network,and vigilance–sensory–motor network.Conclusion:We conclude that the above-mentioned three personality factors are based on brain neural activity,independent of the subjective experience of the personality scale creator,and have stronger explanatory power of brain imaging features.
文摘Background：XB 130 is a recently discovered adaptor protein that is highly expressed in many malignant tumors,but few studies have investigated its role in hepatocellular carcinoma （HCC）.Therefore,this study explored the relationship between this protein and liver cancer and investigated its molecular mechanism of action.Methods：The expression of XB 130 between HCC tissues and adjacent nontumor tissues was compared by real-time polymerase chain reaction,immunochemistry,and Western blotting.XB130 silencing was performed using small hairpin RNA.The effect of silencing XB130 was examined using Cell Counting Kit-8,colony assay,wound healing assay,and cell cycle analysis.Results：We found that XB 130 was highly expressed in HCC tissues （cancer tissues vs.adjacent tissues：0.23 ± 0.02 vs.0.17 ± 0.02,P 〈 0.05） and liver cancer cell lines,particularly MHCC97H and HepG2 （MHCC97H and HepG2 vs.normal liver cell line LO-2：2.35 ± 0.26 and 2.04 ± 0.04 vs.1.00 ± 0.04,respectively,all P 〈 0.05）.The Cell Counting Kit-8 assay,colony formation assay,and xenograft model in nude mice showed that silencing XB130 inhibited cell proliferative ability both in vivo and in vitro,with flow cytometry demonstrating that the cells were arrested in the G0/G1 phase in HepG2 （HepG2 XB130-silenced group [shA] vs.HepG2 scramble group [NA]：74.32 ± 5.86% vs.60.21 ± 3.07%,P 〈 0.05） and that the number of G2/M phase cells was decreased （HepG2 shA vs.HepG2 NA：8.06 ± 2.41% vs.18.36 ± 4.42%,P 〈 0.05）.Furthermore,the cell invasion and migration abilities were impaired,and the levels of the epithelial-mesenchymal transition-related indicators vimentin and N-cadherin were decreased,although the level of E-cadherin was increased after silencingXB130.Western blotting showed that the levels of phosphorylated phosphoinositide 3-kinase （PI3K） and phospho-protein kinase B （p-Akt） also increased,although the level of phosphorylated phosphatase and tensin homolog increased,indicating that XB 130 activated the PI3K/ Akt pathway.Furthermore,we found that a reduction in XB130 increased liver cancer cell sensitivity to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis.Conclusions：Our findings suggest that XB130 might be used as a predictor of liver cancer as well as one of the targets for its treatment.
