Populus 9 euramericana ‘‘Neva'' is the main poplar species in China, where drought stress is becoming extremely urgent. We carried out this research to study the effects of drought stress on the photosynthes...Populus 9 euramericana ‘‘Neva'' is the main poplar species in China, where drought stress is becoming extremely urgent. We carried out this research to study the effects of drought stress on the photosynthesis of Populus 9 euramericana ‘‘Neva''. Drought stress was induced by 58–62%(light), 48–52%(moderate), and 38–42%(severe) relative soil moisture content(RSMC). The effects of drought stress on photosynthetic rate, chlorophyll fluorescence parameters, and other related physiological parameters were investigated during treatment. Net photosynthetic rate(PN), and stomatal conductance decreased significantly and intercellular CO_2 concentration initially increased and then declined, whereas the stomatal limitation factors showed opposite trends in the light under moderate drought stress. Photosystem II(PSII) maximum photochemical efficiency, actual photochemical efficiency,and photochemical quenching decreased gradually under drought stress, whereas nonphotochemical quenching initially increased and then declined. Superoxide dismutase,peroxidase, and catalase activities initially increased and then decreased as RSMC was reduced, whereas malondialdehyde(MDA) content and relative electric conductivity(REC) increased gradually. These results suggest that stomatal factors accounted for the decline in PNunder light and moderate drought stress, whereas leaf PNdecreased mainly due to non-stomatal factors under severe drought stress. PSII was damaged; thus, photosynthetic electron transfer was restricted, indicating that heat dissipation is important for the light protection mechanism of plants.Antioxidant enzymes increased at the beginning of treatment, and the increased MDA and REC led to cell membrane damage. These results suggest that poplar seedlings stabilized their photosynthetic apparatus by reducing the light trapping ability under light and moderate drought stress conditions. This helped dissipate heat and enhance antioxidant enzyme activity. Stomatal factors accounted for the decline in P_N, whereas damage to PSII and antioxidant enzymes under severe drought stress suggested that the decline in P_N was caused by non-stomatal restrictive factors.展开更多
Previous studies showed that Chaetomium globosum ND35 fungus fertilizer can improve the microbial community structure and enzyme activities of replanted soil.However, it remains unclear whether can improve the physiol...Previous studies showed that Chaetomium globosum ND35 fungus fertilizer can improve the microbial community structure and enzyme activities of replanted soil.However, it remains unclear whether can improve the physiological and ecological characteristics of plants under successive rotation.In this study, we investigated the photosynthetic, physiological, and biochemical indexes including photosynthetic parameters, chlorophyll fluorescence, and chlorophyll content of 1-year-old poplar seedlings under seven different doses(range from 0 to1.67 g kg^(-1)) of C.globosum ND35 fungus fertilizer to study the effects of fungus fertilizer on photosynthesis of Poplar.Our results showed that:(1) With increasing application of fungus fertilizer in replanted soil, chlorophyll content of poplar leaves(Chl) increased, while physiological indexes such as electron transport rate(ETR),net photosynthetic rate(P_n), quantum efficiency(U),nitrate reductase(NR) activity and root vigor initially increased and then declined.Meanwhile, heat dissipation that depended on the xanthophyll cycle declined and nonphotochemical quenching(NPQ) initially increased and then decreased.When the dose of C.globosum ND35 fungus fertilizer was 0.67 g kg^(-1)(T3) and 1.00 g kg^(-1)(T4), excess light energy of photosynthetic apparatus was reduced, and photosynthetic apparatus distributed more light energy to the direction of photochemical reactions,which improved the efficiency of energy use.Plant height and biomass of leaves, stems, and roots were maximum at T3.We conclude that applying appropriate amounts of C.globosum ND35 fungus fertilizer can improve root physiological activity and capacity for use of light by poplar leaves.This can improve the operating states of the photosynthetic apparatus and lead to increased photosynthetic efficiency of poplar leaves and accumulation of dry matter.This suggests a strategy to alleviate the successive rotation obstacle of soil nutrient depletion.展开更多
GA20-oxidase (GA20ox) gene encodes a key enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA20ox gene (GeneBank accession number: KU886552) from Paeonia lactiflora. To further reveal i...GA20-oxidase (GA20ox) gene encodes a key enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA20ox gene (GeneBank accession number: KU886552) from Paeonia lactiflora. To further reveal its function, we constructed an expression vector in the present study and then transformed it into Arabidopsis thaliana plants by floral dip method. The transgenic plants exhibited an early bolting, increased height and improved vegetative growth. These results provided efficient vector tool and functional information of PlGA20ox for future gene engineering in peony.展开更多
In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was ...In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.展开更多
Anthocyanin is one of water-soluble natural pigments widely existing in flowers, fruits, stems, leaves and seeds of plants, and it is the major factor conferring pink or red to the petals of Rosa rugose. MYB TFs play ...Anthocyanin is one of water-soluble natural pigments widely existing in flowers, fruits, stems, leaves and seeds of plants, and it is the major factor conferring pink or red to the petals of Rosa rugose. MYB TFs play an important role in the anthocyanin synthesis in plants. This work aimed to clone the MYB gene related to anthocyanin synthesis in the petals of Rosa rugose, and explore the relationship between them to lay a good foundation for gene engineering improvement of R. rugose. Based on the transcriptional data, a full-length cDNA sequence of MYB Gene, RrMYB113 (GenBank accession Nos MG720012), was cloned at the first time from the petals of Rosa rugose “Zi zhi” with RT-PCR and RACE methods. The full-length cDNA is 885 bp with an open reading frame of 654 bp, encoding 216 amino acids. The derived RrMYB113 protein has a molecular weight of 25,297.64 Da, a calculated pI of 9.61, a R2R3-MYB domain and bHLH binding domain, and it also has the signature motifs ((A/S/G)NDV and KPRPR(T/S)), thus belonging to Sg6 R2R3-MYB subfamily. In the secondary structure of RrMYB113 protein, there is 37.04% α-helix, 39.81% random coil, 14.81% extended peptide chain, and 8.33% β-corner. There is no transmembrane domain and no signal peptide cleavage site, seventeen Ser phosphorylation sites, fifteen Thr phosphorylation sites, four Tyr phosphorylation sites, and no O-glycosylation sites. The expression of RrMYB113 increased with the color deepening in petals, and it expressed at a higher level in petals than in other tissues of R. rugose “Zi zhi”. These results are meaningful to reveal that RrMYB113 might be an important regulator in anthocyanin biosynthesis and coloration in the petals of R. rugose.展开更多
R2R3-MYB transcription factor plays an important role in plant anthocyanin synthesis. Based on the transcriptional database of Rosa rugosa, one MYB transcription factor related to floral color, RrMYB6, was cloned. By ...R2R3-MYB transcription factor plays an important role in plant anthocyanin synthesis. Based on the transcriptional database of Rosa rugosa, one MYB transcription factor related to floral color, RrMYB6, was cloned. By using bioinformatics analysis method, cloning MYB gene and analyzing its function in anthocyanin biosynthesis regulation, we hope to lay a solid foundation for new color variety breeding of R. rugosa. Using the R. rugosa “Zi zhi” as the material, we obtained the total length of cDNA of RrMYB6 by RT-PCR and RACE. By analyzing its bioinformatics, we found that the formula of the protein was C1491H2368N452O470S17, molecular weight was 34690.97 Da, the theoretical pI was 8.74. In addition, it belonged to unstable protein with an unstable index at 50.59, and it was also a hydrophilic protein with the total average hydrophobic index at -0.847. In the secondary structure of RrMYB6 protein, the Alpha helix accounted for 32.35%, random coil was 47.39%, extended strand was 11.11%, and beta turn was 9.15%. The sequence analysis showed that RrMYB6 had a typical R2R3-MYB domain and bHLH binding domain, and it also had an N1, C1, C2 inhibitory motif, belonging to the Sg4 subfamily MYB protein. What’s more, evolutionary analysis indicated that the RrMYB6 protein was closely related with the MYB protein in Rosacea family, while it was far from those in other families. The expression analysis showed that RrMYB6 protein decreased with the color of petals deeping, and its expression was the lowest in the petals while the highest in stamens. According to the above results, it was speculated that RrMYB6 was involved in regulating the anthocyanin synthesis of R. rugosa, which belonged to negative regulatory mechanism.展开更多
Mitogen-activated protein kinases (MAPKs) are important components in signal transduction modules which play crucial roles in regulation of many biological processes in plants. Although genome-wide analysis of MAPK an...Mitogen-activated protein kinases (MAPKs) are important components in signal transduction modules which play crucial roles in regulation of many biological processes in plants. Although genome-wide analysis of MAPK and MAPKK family has been carried out in poplar species, few data about the biological function analysis of this gene family are available to date. In this study, a group C MAPK gene 84KMPK14 was cloned from hybrid poplar (Populus alba × P. glandulosa cv. “84K”). It contained a typical protein kinase domain, a conserved TEY-motif and an atypical conserved common docking (CD) domain. Sequence alignment revealed that 84KMPK14 was the most homologous to Populus trichocarpa PtMPK14. Expression analysis indicated that the transcript of 84KMPK14 in roots and young leaves was higher than that in other tissues. The expression of 84KMPK14 was down-regulated by low or high temperature and was induced by H2O2 significantly. It was suppressed by drought and salinity stresses slightly one hour after treatment and then increased quickly three hours after treatment. These results indicated that 84KMPK14 may be involved in environmental stresses, which provides basis for further characterization of the physiological analysis on this gene.展开更多
Based on the transcriptome of Rosa rugosa, one anthocyanin-promoting R2R3-MYB gene, RrMYB10.1 (Accession Nos:MH717244), was cloned from the petals of Rosa rugosa ‘Zizhi’. Sequence analysis results showed that RrMYB1...Based on the transcriptome of Rosa rugosa, one anthocyanin-promoting R2R3-MYB gene, RrMYB10.1 (Accession Nos:MH717244), was cloned from the petals of Rosa rugosa ‘Zizhi’. Sequence analysis results showed that RrMYB10.1 had a full length opening reading frame of 747bp, encoding 249 amino acids. Sequence analysis revealed that RrMYB10.1 contained the conserved R2R3-MYB domain, two atypical anthocyanin-promoting motifs and a conserved amino acid signature for the interaction with bHLH protein. The results of phylogenic tree revealed that RrMYB10.1 showed high homology with other anthocyanin-promoting proteins in Rosacea, and sharing the highest identity (98.39%) with RhMYB10. RT-PCR results showed that RrMYB10.1 was mainly expressed in petals among various tissues and expressed significantly higher in petals in bud stage than in opening period. To sum up, these results showed that RrMYN10.1 may play a key role in regulating anthocyanin concentration, thus providing a certain foundation on regulating flower color formation in Rosa rugosa.展开更多
In order to reveal the phenomenon of R. rugose pollination incompatibility, the full-length cDNA sequence of S Locus F-box Gene was cloned for the first time from the pollen of Rosa rugose “Zilong wochi” with RT-PCR...In order to reveal the phenomenon of R. rugose pollination incompatibility, the full-length cDNA sequence of S Locus F-box Gene was cloned for the first time from the pollen of Rosa rugose “Zilong wochi” with RT-PCR and RACE methods and named as RrSLF. The full-length cDNA is 1236 bp with an open reading frame of 1122 bp, encoding 343 amino acids. The derived protein has a molecular weight of 43.7 kD, a calculated pI of 6.24, an F-box conserved domain at position 343 - 741, and belongs to F-box family. The derived protein is a Hydrophobicity protein secreted into the cytoplasm. There is no transmembrane domain and no signal peptide cleavage site, twenty-one Ser phosphorylation sites, seven Thr phosphorylation sites, seven Tyr phosphorylation sites, two N-glycosylation sites, and no O-glycosylation sites. There are 22.25% α-helixes, 31.37% random coil, 32.17% extended peptide chain, and 14.21% β-corner structure. This protein and the SFB/SLF protein from Rosaceae Prunus fruit, including Prunus speciose, share a sequence homology of 59% - 61%;all of the proteins contain an F-box conserved domain, two hypervariable regions HVa, HVb, and two variable regions V1, V2. Furthermore, their phylogenetic relationships are consistent with their traditional classifications. These results were meaningful to reveal the molecular mechanism of Rosa rugose pollination incompatibility and improve the theory and techniques of breeding ornamental Rosa rugose.展开更多
Under untreated conditions, the germination rate of Viburnum sargentii seeds is very low. By exploring the relationship between the dynamic changes of physicochemical indicators and endogenous hormones and seed germin...Under untreated conditions, the germination rate of Viburnum sargentii seeds is very low. By exploring the relationship between the dynamic changes of physicochemical indicators and endogenous hormones and seed germination during the dormancy and germination of Viburnum sargentii seeds, the mechanism of seed germination of Viburnum sargentii was determined, which provided a theoretical basis for its extensive promotion and development. The contents of soluble sugar and soluble starch were determined by anthrone colorimetry. The soluble protein was determined by Coomassie brilliant blue G-250 staining. The activity of polyphenol oxidase (PPO) was determined by NBT reduction method. The peroxidase (POD) was determined by guaiacol method. And the endogenous hormones ZA, IAA, ABA, GA3 were determined by liquid chromatography. Results show: 1) Under natural conditions, the seeds of Viburnum sargentii are difficult to germinate. 2) Under sand storage for 8 months, the germination rate of untreated seeds was 33%. Puncture and peeling treatment could significantly increase the germination rate, and the germination rate of seeds treated with puncture was 92%, and that treated with peel was 98%. 3) Seed germination was accompanied by the decrease in macromolecular substances such as soluble sugar, soluble starch, and soluble protein. 4) The dynamic changes of hormones during seed germination conform to the hypothesis of “three factors”. 5) The treatment of puncture and peeling increased the content of endogenous hormones promoting germination, decreased the endogenous hormones inhibiting seed germination, and increased the ratio of (IAA + GA + ZR)/ABA or GA/ABA. The seeds of Viburnum sargentii have obvious dormancy characteristics. Under the condition of sand storage, both pricking and peeling treatment can effectively promote the process of breaking dormancy, and the effect of peeling treatment is better.展开更多
One-year Malus hupehensis. var. pingyiensis seedlings potted in soil mixed with 10% or 40% organic fertilizer were treated with two concentrations of lignin (1.5 and 2.5 g·kg-1), and detected the effects of ligni...One-year Malus hupehensis. var. pingyiensis seedlings potted in soil mixed with 10% or 40% organic fertilizer were treated with two concentrations of lignin (1.5 and 2.5 g·kg-1), and detected the effects of lignin on root activity and soil nutrients in rhizosphere soil of Malus hupehensis. var. pingyiensis. (1) Lignin improved root activity of Malus hupehensis. var. pingyiensis, increased soil available nitrogen contents and soil organic matter contents. (2) Under 10% organic fertilizer, lignin improved soil available P and K contents. The root activity in Malus hupehensis. var. pingyiensis was the largest in 2.5 g·kg-1?lignin treatment. (3) Under 40% organic fertilizer, compared with the CK2 (the treatment of soil with organic fertilizer content 40%), lignin decreased the content of rhizosphere soil available K. 1.5 g·kg-1 lignin treatment had obviously increased the content of soil available P. The root activity was the largest in 2.5 g·kg-1 lignin treatment, while the content of rhizosphere soil available K lowered 8.5% compared with that of CK2 in the same treatment. Lignin changed the soil nutrients, 2.5 g·kg-1 lignin significantly improved root activity of Malus hupehensis. var. pingyiensis. The results provided a theoretical basis for the regulation of root activity and soil nutrients in Malus hupehensis. var. pingyiensis.展开更多
In order to determine if the TFL1 is related with the continuous flowering phenotype of wild Rosa rugosa from Muping, the full-length cDNA sequence of TFL1 Gene was cloned for the first time from the flower buds of wi...In order to determine if the TFL1 is related with the continuous flowering phenotype of wild Rosa rugosa from Muping, the full-length cDNA sequence of TFL1 Gene was cloned for the first time from the flower buds of wild Rosa rugosafrom Muping with RT-PCR and RACE methods and named as RrTFL1. The full-length cDNA is 973 bp with an open reading frame of 519 bp, encoding 172 amino acids. The derived protein has a molecular weight of 19.48 kD, a calculated pI of 9.13, a c100227 conserved domain at position 1-172, and belongs to PEBP family. The derived protein is a Hydrophilic protein secreted into the cytoplasmic. There is no transmembrane domain and no signal peptide cleavage site, five Ser phosphorylation sites, seven Thr phosphorylation sites, three Tyr phosphorylation sites, one O-glycosylation site, and no N-glycosylation sites. There are 24.42% α-helixes, 36.63% random coil, 27.91% extended peptide chain, and 11.05% β-corner structure. This protein and the TFL1 protein from Rosaceae plants, including Rosa chinensis, share a sequence homology of 87% - 96%. All of the proteins contain a c100227 conserved domain, two highly conserved modules D-P-D-x-P, G-x-H-R, and two functional sites His, Asp. Furthermore, their phylogenetic relationships are consistent with their traditional classifications. These results not only laid a foundation for further researching the expression and function of RrTFL1, but also cultivating new varieties of R. rugosawhich can flower continuously by gene engineering.展开更多
Plant species produce different types of allelopathic chemicals in nature;however,little is known about the differential regulation of plant allelopathy.Because allelopathy caused by p-hydroxybenzoic acid(pHBA)is one ...Plant species produce different types of allelopathic chemicals in nature;however,little is known about the differential regulation of plant allelopathy.Because allelopathy caused by p-hydroxybenzoic acid(pHBA)is one of the main obstacles to continuous cropping of Populus×euramericana ‘Neva’,we examined gene expression dynamics in Neva leaves induced by pHBA.The expression of genes related to photosynthesis and respiration changed,while mRNA involved in regulating gene expression during allelopathy was degraded.Some miRNAs that are involved in plant allelopathy target crucial genes for regulating reactive oxygen species.Moreover,coexpression regulatory networks were constructed based on profiles of the identified miRNA-target interactions and the differentially expressed miRNA-target pairs.These findings provide a mechanistic framework for understanding allelopathy in plants.展开更多
Background:Black locust(BL,Robinia pseudoacacia)is considered a promising tree species for reforestation due to its great ability to fix nitrogen.However,after two or three coppice-harvesting rotations,the productivit...Background:Black locust(BL,Robinia pseudoacacia)is considered a promising tree species for reforestation due to its great ability to fix nitrogen.However,after two or three coppice-harvesting rotations,the productivity of BL declines.Whether soil microbial communities are affected and how these groups correlate with the nitrogen mineralization process across multi-generation stands remains unclear.Methods:We investigated the composition and structure of free-living nitrogen-fixing microorganisms(diazotrophs)by sequencing the marker gene nifH and compared these results to levels of soil nitrogen mineralization in the bulk soil and rhizosphere in black locust plantations on Mount Tai,China.Results:The results showed multi-generation BL coppice plantations decreased the total soil nitrogen(N),soil phosphorus(P),soil microbial biomass N(MBN),soil microbial biomass C(MBC),soil nitrification rate(Rn),soil ammonification rate(Ra),and net soil N mineralization rate(Rm),but significantly increased the concentration of soil NH_(4þ)-N to maintain sufficient NO_(3)^(-)N.The dominant species in bulk soil and rhizosphere changed from Rhodopseudomonas(22.62%and 15.76%),unclassified_c_Alphaproteobacteria(22.37%and 29.28%),unclassified_o_Rhizobiales(15.40%and 13.31%),Bradyrhizobium(12.00%and 11.74%)in seedling plantations to Bradyrhizobium(45.95%and 47.86%)and Rhodopseudomonas(43.56%and 41.84%)in coppice plantations,respectively.Mantel test and Redundancy analysis(RDA)revealed that Rn,Ra,and Rm were the most important factors shaping the diazotrophic communities.Conclusions:Our results suggest that the multi-generation BL coppice plantation can homogenize soil diazotrophic communities,which is mainly regulated by the available N loss caused by nitrogen mineralization.Strengthening the management technology of coppice plantations will provide more beneficial external consumption.展开更多
In order to find a method to break dormancy of Rosa rugose seeds and interspecific hybrid seeds between Rosa rugose and Rosa hybrid quickly, and accelerate the breeding process of interspecific hybridization between R...In order to find a method to break dormancy of Rosa rugose seeds and interspecific hybrid seeds between Rosa rugose and Rosa hybrid quickly, and accelerate the breeding process of interspecific hybridization between Rosa rugose and Rosa hybrid, the influence of concentrated acid, seed maturity, GA3 (gibberellin) and low temperature (4°C) on seed germination of Rosa rugose from Muping was researched under aseptic condition. The results showed that aseptic germination can significantly shorten the germination time of Rosa rugose seeds and raise its germination ratio. Before inoculation, concentrated acid treatment greatly increased the germination rate and reduced the contamination rate of the seeds. The higher the degree of maturity of seeds is, the lower the germination rate would be, and the best time for seed to aseptic germination is 60 d after pollination. The addition of GA3 in 1/2MS medium could promote seeds germination better, and when the concentration of GA3 was 0.15 mg/L, the seed germination ratio was the highest;the germination time decreased and the seed germination ratio increased gradually as the treatment time at 4°C lasted longer.展开更多
In order to realize the efficient industrial production of <i>Ilex</i> “China Girl”, an orthogonal experiment with 4 factors and 3 levels was designed. Firstly, the optimal orthogonal cutting scheme was ...In order to realize the efficient industrial production of <i>Ilex</i> “China Girl”, an orthogonal experiment with 4 factors and 3 levels was designed. Firstly, the optimal orthogonal cutting scheme was selected from 9 treatments. Then through the systematic analysis of the effects of cutting position, substrate, exogenous hormones type and concentration on rooting indexes, such as rooting rate, root number, root length and root effect index, the theoretical optimal scheme was predicted and verified. The results showed that the theoretical optimal scheme (3000 mg/L IBA treatment for 15 s, cutting in mixed matrix with peat soil, perlite and vermiculite ratio of 2:1:1) was the optimal <span>cutting rooting scheme of <i>Ilex</i> “China Girl”. After the treatment of this sc</span>heme, the rooting rate of <i>Ilex</i> “China Girl” reached 100%, the average root number was 51.67 per plant, and the average root length was 6.13 cm. The rooting time was greatly shortened, the rooting rate and rooting effect were greatly improved. In this study, the efficient cutting propagation technology system of <i>Ilex</i> “China Girl” was established, which laid a foundation for the popularization and application of <i>Ilex</i> “China Girl”, and also provided reference for further improving the cutting propagation efficiency of other evergreen holly. This study laid a foundation for the application of <i>Ilex</i> “China Girl”, and also provided a reference for further improving the cutting propagation efficiency of other evergreen holly.展开更多
As the largest sub-basin of the middle reaches of the Yellow River with an obvious decreasing trend in annual runoff in recent years,the Weihe River basin is a significant region with regard to the protection and impr...As the largest sub-basin of the middle reaches of the Yellow River with an obvious decreasing trend in annual runoff in recent years,the Weihe River basin is a significant region with regard to the protection and improvement of the environment in West China.Evapotranspiration(ET)is the loss of water from the Earth’s surface to the atmosphere and plays an important role in the regional water cycle,especially when considering water resource shortages.In this study,through analyzing the grid precipitation data after interpolation from 39 meteorological stations in and around the Weihe River basin from 1981 to 2011,certain periods during 1987,1993,1999,2001,2002 and 2009 with similar precipitation characteristics had been chosen for estimating the ET in the Weihe River basin.To illustrate ET’s influence on the water budget,these estimations are calculated based on an improved Penman-Monteith equation as well as remote sensing data and meteorological data.The results show that:(1)the annual ET in the Weihe River basin ranged from 350mm to 400mm in 1987,1993,1999,2001,2002 and 2009,accounting for more than 70%of the corresponding annual precipitation.There is a definite increasing trend in different decades that is primarily distributed during the summer.(2)The spatial distribution patterns of the ET in the six years mentioned area unique set,and the years are roughly identical with more than 500mm in the middle and lower reaches of the Weihe River in the southeastern region and less than 400mm in upper reaches of the Jinghe River in the northwestern area.(3)At the single-point scale,the coefficient of determination(R2)is 0.618 compared to the eddy correlation measurements in 2009 at the Changwu site,showing good agreement between the estimated ET and the observed ET.At the basin scale,the model-estimated ET is slightly lower than the actual ET with regard to the surface water budget.Additionally,the estimated ET in 2001,2002 and 2009 is close to the MODIS ET product.(4)For similar precipitation conditions,the regional amount of water shows a decreasing tendency with increasing ET,which may result from the rise in NDVI and improvements in vegetation coverage caused by human activities.This research suggests the influence of ET on water change at the basin level,which can also explain the decreasing runoff and can provide necessary information for improved water resource management.展开更多
Physical characteristics of soil structure and soil moisture of five kinds of artificial forests were researched in Shengquan Temple Forestry Farm, in the southern mountainous area of Jinan City, in order to offer sci...Physical characteristics of soil structure and soil moisture of five kinds of artificial forests were researched in Shengquan Temple Forestry Farm, in the southern mountainous area of Jinan City, in order to offer scientific management and nurture of forest vegetation in mountainous area of North China. Results indicated that: (1) In these artificial forests researched, some indexes expressing soil pedonydrological physical character, such as soil density, porosity, infiltration rate, were much better than those in bare land, and these indexes showed this order: mixed forest > pure forest, Kalopanax pictus > Koelreuteria paniculata > Pistacia chinensis. (2) In dry and rainy seasons, with the increasing of soil depth, soil water content decreased in most parts of forest, and their extent of change was less than those in bare land. (3) With the increasing of soil depth, soil saturated water content decreased in all kinds of forests. Water storing capacity of surface soil layer was the best in the mixed forest, while differences were little in other forest. Water storing and adjusting capacity was good in the mixed forest, while water offering capacity had little discrepancy in all forests. Conclusions show that judging from water conservation and water use efficiency, mixed forest is better than pure forest, and in pure forest K. pictus is better than K. paniculata. Therefore, it is proposed that in the future, mixed forest should be fostered and managed firstly, the species being made up of K. pictus and P. chinensis. In the pure forest, K. pictus is much better than others. Nowadays K. paniculata, and P. chinensis, especially their soil surface vegetation and litter, should be protected. As to poor forest, closing hill for afforestation should be adopted. Poor forest have better be reconstructed to be mixed forest in available conditions.展开更多
Tree peony(Paeonia suffruticosa)is an ornamental and medicinal plant in China.The fresh eating of flower petals has gradually become a new idea for further development.This study analyzed the sensory indexes,nutrition...Tree peony(Paeonia suffruticosa)is an ornamental and medicinal plant in China.The fresh eating of flower petals has gradually become a new idea for further development.This study analyzed the sensory indexes,nutritional indexes and antioxidant indexes of fresh flowers of five cultivars,namely,‘Fengdan’(P.ostia‘Fengdan’),‘Xiangyu’(P.suffruticosa‘Xiangyu’),‘Zhaofen’(P.suffruticosa‘Zhanfen’),‘Luoyanghong’(P.suffruticosa‘Luoyanghong’)and‘High Noon’(P.‘High Noon’),to provide a theoretical basis for eating fresh tree peony flowers.The aroma volatiles of petals mainly comprised terpenes,alcohols and alkanes.‘Luoyanghong’and‘High Noon’have brighter colors.The taste test showed that‘Luoyanghong’,‘High Noon’and‘Xiangyu’were the most palatable.‘High Noon’contained the highest soluble sugar content,‘Luoyanghong’contained the highest starch and vitamin C(VC)levels,and‘Zhaofen’contained the highest soluble protein content.The contents of bioactive compounds were the highest in‘Luoyanghong’,and superoxide dismutase(SOD)activity was the highest in‘Xiangyu’.The petals were rich in essential amino acids,with the highest amount in‘Xiangyu’.‘Luoyanghong’and‘High Noon’showed strong antioxidant capacity.Therefore,tree peony flowers have excellent sensory qualities,abundant nutrients and strong antioxidant activity and could be further developed as a food to be eaten fresh.The results showed that tree peony flowers had great edible potential.Flowers of red and yellow cultivars can be considered for fresh eating on the basis of the traditional consumption of white and pink tree peony flowers.展开更多
基金financially supported by the National Public Welfare Industry Research Project of China(201504406)the National Natural Science Foundation of China(31770706)
文摘Populus 9 euramericana ‘‘Neva'' is the main poplar species in China, where drought stress is becoming extremely urgent. We carried out this research to study the effects of drought stress on the photosynthesis of Populus 9 euramericana ‘‘Neva''. Drought stress was induced by 58–62%(light), 48–52%(moderate), and 38–42%(severe) relative soil moisture content(RSMC). The effects of drought stress on photosynthetic rate, chlorophyll fluorescence parameters, and other related physiological parameters were investigated during treatment. Net photosynthetic rate(PN), and stomatal conductance decreased significantly and intercellular CO_2 concentration initially increased and then declined, whereas the stomatal limitation factors showed opposite trends in the light under moderate drought stress. Photosystem II(PSII) maximum photochemical efficiency, actual photochemical efficiency,and photochemical quenching decreased gradually under drought stress, whereas nonphotochemical quenching initially increased and then declined. Superoxide dismutase,peroxidase, and catalase activities initially increased and then decreased as RSMC was reduced, whereas malondialdehyde(MDA) content and relative electric conductivity(REC) increased gradually. These results suggest that stomatal factors accounted for the decline in PNunder light and moderate drought stress, whereas leaf PNdecreased mainly due to non-stomatal factors under severe drought stress. PSII was damaged; thus, photosynthetic electron transfer was restricted, indicating that heat dissipation is important for the light protection mechanism of plants.Antioxidant enzymes increased at the beginning of treatment, and the increased MDA and REC led to cell membrane damage. These results suggest that poplar seedlings stabilized their photosynthetic apparatus by reducing the light trapping ability under light and moderate drought stress conditions. This helped dissipate heat and enhance antioxidant enzyme activity. Stomatal factors accounted for the decline in P_N, whereas damage to PSII and antioxidant enzymes under severe drought stress suggested that the decline in P_N was caused by non-stomatal restrictive factors.
基金supported by the China“973”projects(No.2012CB416904)the National Natural Science Foundation of China(Nos.31770706,31370702,31500511)+1 种基金the research and demonstration on the key technology of vegetation restoration and reconstruction in the open pit of in eastern Shandong hilly area(201504406)the Key Project of Natural Science Foundation of Shandong Province,China(Nos.ZR2015JL014,ZR2015CL044)
文摘Previous studies showed that Chaetomium globosum ND35 fungus fertilizer can improve the microbial community structure and enzyme activities of replanted soil.However, it remains unclear whether can improve the physiological and ecological characteristics of plants under successive rotation.In this study, we investigated the photosynthetic, physiological, and biochemical indexes including photosynthetic parameters, chlorophyll fluorescence, and chlorophyll content of 1-year-old poplar seedlings under seven different doses(range from 0 to1.67 g kg^(-1)) of C.globosum ND35 fungus fertilizer to study the effects of fungus fertilizer on photosynthesis of Poplar.Our results showed that:(1) With increasing application of fungus fertilizer in replanted soil, chlorophyll content of poplar leaves(Chl) increased, while physiological indexes such as electron transport rate(ETR),net photosynthetic rate(P_n), quantum efficiency(U),nitrate reductase(NR) activity and root vigor initially increased and then declined.Meanwhile, heat dissipation that depended on the xanthophyll cycle declined and nonphotochemical quenching(NPQ) initially increased and then decreased.When the dose of C.globosum ND35 fungus fertilizer was 0.67 g kg^(-1)(T3) and 1.00 g kg^(-1)(T4), excess light energy of photosynthetic apparatus was reduced, and photosynthetic apparatus distributed more light energy to the direction of photochemical reactions,which improved the efficiency of energy use.Plant height and biomass of leaves, stems, and roots were maximum at T3.We conclude that applying appropriate amounts of C.globosum ND35 fungus fertilizer can improve root physiological activity and capacity for use of light by poplar leaves.This can improve the operating states of the photosynthetic apparatus and lead to increased photosynthetic efficiency of poplar leaves and accumulation of dry matter.This suggests a strategy to alleviate the successive rotation obstacle of soil nutrient depletion.
文摘GA20-oxidase (GA20ox) gene encodes a key enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA20ox gene (GeneBank accession number: KU886552) from Paeonia lactiflora. To further reveal its function, we constructed an expression vector in the present study and then transformed it into Arabidopsis thaliana plants by floral dip method. The transgenic plants exhibited an early bolting, increased height and improved vegetative growth. These results provided efficient vector tool and functional information of PlGA20ox for future gene engineering in peony.
文摘In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.
文摘Anthocyanin is one of water-soluble natural pigments widely existing in flowers, fruits, stems, leaves and seeds of plants, and it is the major factor conferring pink or red to the petals of Rosa rugose. MYB TFs play an important role in the anthocyanin synthesis in plants. This work aimed to clone the MYB gene related to anthocyanin synthesis in the petals of Rosa rugose, and explore the relationship between them to lay a good foundation for gene engineering improvement of R. rugose. Based on the transcriptional data, a full-length cDNA sequence of MYB Gene, RrMYB113 (GenBank accession Nos MG720012), was cloned at the first time from the petals of Rosa rugose “Zi zhi” with RT-PCR and RACE methods. The full-length cDNA is 885 bp with an open reading frame of 654 bp, encoding 216 amino acids. The derived RrMYB113 protein has a molecular weight of 25,297.64 Da, a calculated pI of 9.61, a R2R3-MYB domain and bHLH binding domain, and it also has the signature motifs ((A/S/G)NDV and KPRPR(T/S)), thus belonging to Sg6 R2R3-MYB subfamily. In the secondary structure of RrMYB113 protein, there is 37.04% α-helix, 39.81% random coil, 14.81% extended peptide chain, and 8.33% β-corner. There is no transmembrane domain and no signal peptide cleavage site, seventeen Ser phosphorylation sites, fifteen Thr phosphorylation sites, four Tyr phosphorylation sites, and no O-glycosylation sites. The expression of RrMYB113 increased with the color deepening in petals, and it expressed at a higher level in petals than in other tissues of R. rugose “Zi zhi”. These results are meaningful to reveal that RrMYB113 might be an important regulator in anthocyanin biosynthesis and coloration in the petals of R. rugose.
文摘R2R3-MYB transcription factor plays an important role in plant anthocyanin synthesis. Based on the transcriptional database of Rosa rugosa, one MYB transcription factor related to floral color, RrMYB6, was cloned. By using bioinformatics analysis method, cloning MYB gene and analyzing its function in anthocyanin biosynthesis regulation, we hope to lay a solid foundation for new color variety breeding of R. rugosa. Using the R. rugosa “Zi zhi” as the material, we obtained the total length of cDNA of RrMYB6 by RT-PCR and RACE. By analyzing its bioinformatics, we found that the formula of the protein was C1491H2368N452O470S17, molecular weight was 34690.97 Da, the theoretical pI was 8.74. In addition, it belonged to unstable protein with an unstable index at 50.59, and it was also a hydrophilic protein with the total average hydrophobic index at -0.847. In the secondary structure of RrMYB6 protein, the Alpha helix accounted for 32.35%, random coil was 47.39%, extended strand was 11.11%, and beta turn was 9.15%. The sequence analysis showed that RrMYB6 had a typical R2R3-MYB domain and bHLH binding domain, and it also had an N1, C1, C2 inhibitory motif, belonging to the Sg4 subfamily MYB protein. What’s more, evolutionary analysis indicated that the RrMYB6 protein was closely related with the MYB protein in Rosacea family, while it was far from those in other families. The expression analysis showed that RrMYB6 protein decreased with the color of petals deeping, and its expression was the lowest in the petals while the highest in stamens. According to the above results, it was speculated that RrMYB6 was involved in regulating the anthocyanin synthesis of R. rugosa, which belonged to negative regulatory mechanism.
文摘Mitogen-activated protein kinases (MAPKs) are important components in signal transduction modules which play crucial roles in regulation of many biological processes in plants. Although genome-wide analysis of MAPK and MAPKK family has been carried out in poplar species, few data about the biological function analysis of this gene family are available to date. In this study, a group C MAPK gene 84KMPK14 was cloned from hybrid poplar (Populus alba × P. glandulosa cv. “84K”). It contained a typical protein kinase domain, a conserved TEY-motif and an atypical conserved common docking (CD) domain. Sequence alignment revealed that 84KMPK14 was the most homologous to Populus trichocarpa PtMPK14. Expression analysis indicated that the transcript of 84KMPK14 in roots and young leaves was higher than that in other tissues. The expression of 84KMPK14 was down-regulated by low or high temperature and was induced by H2O2 significantly. It was suppressed by drought and salinity stresses slightly one hour after treatment and then increased quickly three hours after treatment. These results indicated that 84KMPK14 may be involved in environmental stresses, which provides basis for further characterization of the physiological analysis on this gene.
文摘Based on the transcriptome of Rosa rugosa, one anthocyanin-promoting R2R3-MYB gene, RrMYB10.1 (Accession Nos:MH717244), was cloned from the petals of Rosa rugosa ‘Zizhi’. Sequence analysis results showed that RrMYB10.1 had a full length opening reading frame of 747bp, encoding 249 amino acids. Sequence analysis revealed that RrMYB10.1 contained the conserved R2R3-MYB domain, two atypical anthocyanin-promoting motifs and a conserved amino acid signature for the interaction with bHLH protein. The results of phylogenic tree revealed that RrMYB10.1 showed high homology with other anthocyanin-promoting proteins in Rosacea, and sharing the highest identity (98.39%) with RhMYB10. RT-PCR results showed that RrMYB10.1 was mainly expressed in petals among various tissues and expressed significantly higher in petals in bud stage than in opening period. To sum up, these results showed that RrMYN10.1 may play a key role in regulating anthocyanin concentration, thus providing a certain foundation on regulating flower color formation in Rosa rugosa.
文摘In order to reveal the phenomenon of R. rugose pollination incompatibility, the full-length cDNA sequence of S Locus F-box Gene was cloned for the first time from the pollen of Rosa rugose “Zilong wochi” with RT-PCR and RACE methods and named as RrSLF. The full-length cDNA is 1236 bp with an open reading frame of 1122 bp, encoding 343 amino acids. The derived protein has a molecular weight of 43.7 kD, a calculated pI of 6.24, an F-box conserved domain at position 343 - 741, and belongs to F-box family. The derived protein is a Hydrophobicity protein secreted into the cytoplasm. There is no transmembrane domain and no signal peptide cleavage site, twenty-one Ser phosphorylation sites, seven Thr phosphorylation sites, seven Tyr phosphorylation sites, two N-glycosylation sites, and no O-glycosylation sites. There are 22.25% α-helixes, 31.37% random coil, 32.17% extended peptide chain, and 14.21% β-corner structure. This protein and the SFB/SLF protein from Rosaceae Prunus fruit, including Prunus speciose, share a sequence homology of 59% - 61%;all of the proteins contain an F-box conserved domain, two hypervariable regions HVa, HVb, and two variable regions V1, V2. Furthermore, their phylogenetic relationships are consistent with their traditional classifications. These results were meaningful to reveal the molecular mechanism of Rosa rugose pollination incompatibility and improve the theory and techniques of breeding ornamental Rosa rugose.
文摘Under untreated conditions, the germination rate of Viburnum sargentii seeds is very low. By exploring the relationship between the dynamic changes of physicochemical indicators and endogenous hormones and seed germination during the dormancy and germination of Viburnum sargentii seeds, the mechanism of seed germination of Viburnum sargentii was determined, which provided a theoretical basis for its extensive promotion and development. The contents of soluble sugar and soluble starch were determined by anthrone colorimetry. The soluble protein was determined by Coomassie brilliant blue G-250 staining. The activity of polyphenol oxidase (PPO) was determined by NBT reduction method. The peroxidase (POD) was determined by guaiacol method. And the endogenous hormones ZA, IAA, ABA, GA3 were determined by liquid chromatography. Results show: 1) Under natural conditions, the seeds of Viburnum sargentii are difficult to germinate. 2) Under sand storage for 8 months, the germination rate of untreated seeds was 33%. Puncture and peeling treatment could significantly increase the germination rate, and the germination rate of seeds treated with puncture was 92%, and that treated with peel was 98%. 3) Seed germination was accompanied by the decrease in macromolecular substances such as soluble sugar, soluble starch, and soluble protein. 4) The dynamic changes of hormones during seed germination conform to the hypothesis of “three factors”. 5) The treatment of puncture and peeling increased the content of endogenous hormones promoting germination, decreased the endogenous hormones inhibiting seed germination, and increased the ratio of (IAA + GA + ZR)/ABA or GA/ABA. The seeds of Viburnum sargentii have obvious dormancy characteristics. Under the condition of sand storage, both pricking and peeling treatment can effectively promote the process of breaking dormancy, and the effect of peeling treatment is better.
文摘One-year Malus hupehensis. var. pingyiensis seedlings potted in soil mixed with 10% or 40% organic fertilizer were treated with two concentrations of lignin (1.5 and 2.5 g·kg-1), and detected the effects of lignin on root activity and soil nutrients in rhizosphere soil of Malus hupehensis. var. pingyiensis. (1) Lignin improved root activity of Malus hupehensis. var. pingyiensis, increased soil available nitrogen contents and soil organic matter contents. (2) Under 10% organic fertilizer, lignin improved soil available P and K contents. The root activity in Malus hupehensis. var. pingyiensis was the largest in 2.5 g·kg-1?lignin treatment. (3) Under 40% organic fertilizer, compared with the CK2 (the treatment of soil with organic fertilizer content 40%), lignin decreased the content of rhizosphere soil available K. 1.5 g·kg-1 lignin treatment had obviously increased the content of soil available P. The root activity was the largest in 2.5 g·kg-1 lignin treatment, while the content of rhizosphere soil available K lowered 8.5% compared with that of CK2 in the same treatment. Lignin changed the soil nutrients, 2.5 g·kg-1 lignin significantly improved root activity of Malus hupehensis. var. pingyiensis. The results provided a theoretical basis for the regulation of root activity and soil nutrients in Malus hupehensis. var. pingyiensis.
文摘In order to determine if the TFL1 is related with the continuous flowering phenotype of wild Rosa rugosa from Muping, the full-length cDNA sequence of TFL1 Gene was cloned for the first time from the flower buds of wild Rosa rugosafrom Muping with RT-PCR and RACE methods and named as RrTFL1. The full-length cDNA is 973 bp with an open reading frame of 519 bp, encoding 172 amino acids. The derived protein has a molecular weight of 19.48 kD, a calculated pI of 9.13, a c100227 conserved domain at position 1-172, and belongs to PEBP family. The derived protein is a Hydrophilic protein secreted into the cytoplasmic. There is no transmembrane domain and no signal peptide cleavage site, five Ser phosphorylation sites, seven Thr phosphorylation sites, three Tyr phosphorylation sites, one O-glycosylation site, and no N-glycosylation sites. There are 24.42% α-helixes, 36.63% random coil, 27.91% extended peptide chain, and 11.05% β-corner structure. This protein and the TFL1 protein from Rosaceae plants, including Rosa chinensis, share a sequence homology of 87% - 96%. All of the proteins contain a c100227 conserved domain, two highly conserved modules D-P-D-x-P, G-x-H-R, and two functional sites His, Asp. Furthermore, their phylogenetic relationships are consistent with their traditional classifications. These results not only laid a foundation for further researching the expression and function of RrTFL1, but also cultivating new varieties of R. rugosawhich can flower continuously by gene engineering.
基金the National Public Welfare Industry Research Project of China(201504406)National Natural Science Foundation of China(31770706)+1 种基金National Natural Science Foundation of China(31870379)Science Research Projects of Facility Horticulture Laboratory of Universities in Shandong(2018YY031)。
文摘Plant species produce different types of allelopathic chemicals in nature;however,little is known about the differential regulation of plant allelopathy.Because allelopathy caused by p-hydroxybenzoic acid(pHBA)is one of the main obstacles to continuous cropping of Populus×euramericana ‘Neva’,we examined gene expression dynamics in Neva leaves induced by pHBA.The expression of genes related to photosynthesis and respiration changed,while mRNA involved in regulating gene expression during allelopathy was degraded.Some miRNAs that are involved in plant allelopathy target crucial genes for regulating reactive oxygen species.Moreover,coexpression regulatory networks were constructed based on profiles of the identified miRNA-target interactions and the differentially expressed miRNA-target pairs.These findings provide a mechanistic framework for understanding allelopathy in plants.
基金supported by the Agricultural Science and Technology Fund for Forestry Sci-tech Innovation Project of Shandong Province (No.2019LY005)the National Natural Science Foundation of China (No.31570705)+1 种基金Shandong Province Higher School Science and Technology Plan Project (No. J16LF09)and Shandong Province Natural Science Foundation (No. ZR2018PC006)
文摘Background:Black locust(BL,Robinia pseudoacacia)is considered a promising tree species for reforestation due to its great ability to fix nitrogen.However,after two or three coppice-harvesting rotations,the productivity of BL declines.Whether soil microbial communities are affected and how these groups correlate with the nitrogen mineralization process across multi-generation stands remains unclear.Methods:We investigated the composition and structure of free-living nitrogen-fixing microorganisms(diazotrophs)by sequencing the marker gene nifH and compared these results to levels of soil nitrogen mineralization in the bulk soil and rhizosphere in black locust plantations on Mount Tai,China.Results:The results showed multi-generation BL coppice plantations decreased the total soil nitrogen(N),soil phosphorus(P),soil microbial biomass N(MBN),soil microbial biomass C(MBC),soil nitrification rate(Rn),soil ammonification rate(Ra),and net soil N mineralization rate(Rm),but significantly increased the concentration of soil NH_(4þ)-N to maintain sufficient NO_(3)^(-)N.The dominant species in bulk soil and rhizosphere changed from Rhodopseudomonas(22.62%and 15.76%),unclassified_c_Alphaproteobacteria(22.37%and 29.28%),unclassified_o_Rhizobiales(15.40%and 13.31%),Bradyrhizobium(12.00%and 11.74%)in seedling plantations to Bradyrhizobium(45.95%and 47.86%)and Rhodopseudomonas(43.56%and 41.84%)in coppice plantations,respectively.Mantel test and Redundancy analysis(RDA)revealed that Rn,Ra,and Rm were the most important factors shaping the diazotrophic communities.Conclusions:Our results suggest that the multi-generation BL coppice plantation can homogenize soil diazotrophic communities,which is mainly regulated by the available N loss caused by nitrogen mineralization.Strengthening the management technology of coppice plantations will provide more beneficial external consumption.
文摘In order to find a method to break dormancy of Rosa rugose seeds and interspecific hybrid seeds between Rosa rugose and Rosa hybrid quickly, and accelerate the breeding process of interspecific hybridization between Rosa rugose and Rosa hybrid, the influence of concentrated acid, seed maturity, GA3 (gibberellin) and low temperature (4°C) on seed germination of Rosa rugose from Muping was researched under aseptic condition. The results showed that aseptic germination can significantly shorten the germination time of Rosa rugose seeds and raise its germination ratio. Before inoculation, concentrated acid treatment greatly increased the germination rate and reduced the contamination rate of the seeds. The higher the degree of maturity of seeds is, the lower the germination rate would be, and the best time for seed to aseptic germination is 60 d after pollination. The addition of GA3 in 1/2MS medium could promote seeds germination better, and when the concentration of GA3 was 0.15 mg/L, the seed germination ratio was the highest;the germination time decreased and the seed germination ratio increased gradually as the treatment time at 4°C lasted longer.
文摘In order to realize the efficient industrial production of <i>Ilex</i> “China Girl”, an orthogonal experiment with 4 factors and 3 levels was designed. Firstly, the optimal orthogonal cutting scheme was selected from 9 treatments. Then through the systematic analysis of the effects of cutting position, substrate, exogenous hormones type and concentration on rooting indexes, such as rooting rate, root number, root length and root effect index, the theoretical optimal scheme was predicted and verified. The results showed that the theoretical optimal scheme (3000 mg/L IBA treatment for 15 s, cutting in mixed matrix with peat soil, perlite and vermiculite ratio of 2:1:1) was the optimal <span>cutting rooting scheme of <i>Ilex</i> “China Girl”. After the treatment of this sc</span>heme, the rooting rate of <i>Ilex</i> “China Girl” reached 100%, the average root number was 51.67 per plant, and the average root length was 6.13 cm. The rooting time was greatly shortened, the rooting rate and rooting effect were greatly improved. In this study, the efficient cutting propagation technology system of <i>Ilex</i> “China Girl” was established, which laid a foundation for the popularization and application of <i>Ilex</i> “China Girl”, and also provided reference for further improving the cutting propagation efficiency of other evergreen holly. This study laid a foundation for the application of <i>Ilex</i> “China Girl”, and also provided a reference for further improving the cutting propagation efficiency of other evergreen holly.
文摘As the largest sub-basin of the middle reaches of the Yellow River with an obvious decreasing trend in annual runoff in recent years,the Weihe River basin is a significant region with regard to the protection and improvement of the environment in West China.Evapotranspiration(ET)is the loss of water from the Earth’s surface to the atmosphere and plays an important role in the regional water cycle,especially when considering water resource shortages.In this study,through analyzing the grid precipitation data after interpolation from 39 meteorological stations in and around the Weihe River basin from 1981 to 2011,certain periods during 1987,1993,1999,2001,2002 and 2009 with similar precipitation characteristics had been chosen for estimating the ET in the Weihe River basin.To illustrate ET’s influence on the water budget,these estimations are calculated based on an improved Penman-Monteith equation as well as remote sensing data and meteorological data.The results show that:(1)the annual ET in the Weihe River basin ranged from 350mm to 400mm in 1987,1993,1999,2001,2002 and 2009,accounting for more than 70%of the corresponding annual precipitation.There is a definite increasing trend in different decades that is primarily distributed during the summer.(2)The spatial distribution patterns of the ET in the six years mentioned area unique set,and the years are roughly identical with more than 500mm in the middle and lower reaches of the Weihe River in the southeastern region and less than 400mm in upper reaches of the Jinghe River in the northwestern area.(3)At the single-point scale,the coefficient of determination(R2)is 0.618 compared to the eddy correlation measurements in 2009 at the Changwu site,showing good agreement between the estimated ET and the observed ET.At the basin scale,the model-estimated ET is slightly lower than the actual ET with regard to the surface water budget.Additionally,the estimated ET in 2001,2002 and 2009 is close to the MODIS ET product.(4)For similar precipitation conditions,the regional amount of water shows a decreasing tendency with increasing ET,which may result from the rise in NDVI and improvements in vegetation coverage caused by human activities.This research suggests the influence of ET on water change at the basin level,which can also explain the decreasing runoff and can provide necessary information for improved water resource management.
基金The project was supported by national key science and technology item in "10th five-year" granted by the National Natural Science Foundation of China(2001BA510B0404).
文摘Physical characteristics of soil structure and soil moisture of five kinds of artificial forests were researched in Shengquan Temple Forestry Farm, in the southern mountainous area of Jinan City, in order to offer scientific management and nurture of forest vegetation in mountainous area of North China. Results indicated that: (1) In these artificial forests researched, some indexes expressing soil pedonydrological physical character, such as soil density, porosity, infiltration rate, were much better than those in bare land, and these indexes showed this order: mixed forest > pure forest, Kalopanax pictus > Koelreuteria paniculata > Pistacia chinensis. (2) In dry and rainy seasons, with the increasing of soil depth, soil water content decreased in most parts of forest, and their extent of change was less than those in bare land. (3) With the increasing of soil depth, soil saturated water content decreased in all kinds of forests. Water storing capacity of surface soil layer was the best in the mixed forest, while differences were little in other forest. Water storing and adjusting capacity was good in the mixed forest, while water offering capacity had little discrepancy in all forests. Conclusions show that judging from water conservation and water use efficiency, mixed forest is better than pure forest, and in pure forest K. pictus is better than K. paniculata. Therefore, it is proposed that in the future, mixed forest should be fostered and managed firstly, the species being made up of K. pictus and P. chinensis. In the pure forest, K. pictus is much better than others. Nowadays K. paniculata, and P. chinensis, especially their soil surface vegetation and litter, should be protected. As to poor forest, closing hill for afforestation should be adopted. Poor forest have better be reconstructed to be mixed forest in available conditions.
基金financially supported by the Agricultural Science and Technology Fund for Forestry Sci-tech Innovation Project of Shandong Province(2019LY005LYCX07-2018-37)The funding agency is the Department of science and technology of Shandong Province.
文摘Tree peony(Paeonia suffruticosa)is an ornamental and medicinal plant in China.The fresh eating of flower petals has gradually become a new idea for further development.This study analyzed the sensory indexes,nutritional indexes and antioxidant indexes of fresh flowers of five cultivars,namely,‘Fengdan’(P.ostia‘Fengdan’),‘Xiangyu’(P.suffruticosa‘Xiangyu’),‘Zhaofen’(P.suffruticosa‘Zhanfen’),‘Luoyanghong’(P.suffruticosa‘Luoyanghong’)and‘High Noon’(P.‘High Noon’),to provide a theoretical basis for eating fresh tree peony flowers.The aroma volatiles of petals mainly comprised terpenes,alcohols and alkanes.‘Luoyanghong’and‘High Noon’have brighter colors.The taste test showed that‘Luoyanghong’,‘High Noon’and‘Xiangyu’were the most palatable.‘High Noon’contained the highest soluble sugar content,‘Luoyanghong’contained the highest starch and vitamin C(VC)levels,and‘Zhaofen’contained the highest soluble protein content.The contents of bioactive compounds were the highest in‘Luoyanghong’,and superoxide dismutase(SOD)activity was the highest in‘Xiangyu’.The petals were rich in essential amino acids,with the highest amount in‘Xiangyu’.‘Luoyanghong’and‘High Noon’showed strong antioxidant capacity.Therefore,tree peony flowers have excellent sensory qualities,abundant nutrients and strong antioxidant activity and could be further developed as a food to be eaten fresh.The results showed that tree peony flowers had great edible potential.Flowers of red and yellow cultivars can be considered for fresh eating on the basis of the traditional consumption of white and pink tree peony flowers.
