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Comparative analysis of volatile compounds in different muskmelon cultivars in Xinjiang based on HS-SPME-GC-MS and transcriptomics
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作者 Yingying Fan Binxin Jia +5 位作者 Xiaoqian Cao Jun Yang Xiaolong Li Weizhong He Fengjuan Liu Cheng Wang 《Food Innovation and Advances》 2023年第3期217-224,共8页
Volatile flavor has prompted a great amount of influence in acceptance and view points in fruit products.Melon(Pyrus communis)is an aromadense fruit,thus,the evaluation of volatile flavor is crucial to melon-breeding.... Volatile flavor has prompted a great amount of influence in acceptance and view points in fruit products.Melon(Pyrus communis)is an aromadense fruit,thus,the evaluation of volatile flavor is crucial to melon-breeding.The volatile compounds present in nine varieties of Xinjiang muskmelons were identified and analyzed using the headspace solid-phase microextraction and gas chromatography-mass spectrometry methods.In addition,transcriptomics were used to discover the differential genes in fatty acid degradation pathways.It was found that a total of 170 volatile substances,including 52 alcohols,41 esters,24 aldehydes,32 ketones,14 acids and seven phenols,were identified in the nine melons.Results of PCA showed that 3-nonanol,2-nonanol,bis(2-ethylhexyl)adipate,and 2-methylpropanal contributed more to the flavor of melon.It was verified that high activities of acyl-coenzyme A cholesterol acyltransferase(AAT)promoted the conversion of alcohols to esters,so that the melons have a high content of esters.Four genes of long-chain acyl-CoA synthetase were mainly responsible for the large difference in volatile substances.This practice may further undermine the primary rationale for the breeding and promotion in different cultivars of muskmelon. 展开更多
关键词 volatile ESTERS ALCOHOLS
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Use of Asian selected agricultural byproducts to modulate rumen microbes and fermentation 被引量:3
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作者 Yasuo Kobayashi Seongjin Oh +1 位作者 Htun Myint Satoshi Koike 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2017年第2期317-326,共10页
In the last five decades, attempts have been made to improve rumen fermentation and host animal nutrition through modulation of rumen microbiota. The goals have been decreasing methane production, partially inhibiting... In the last five decades, attempts have been made to improve rumen fermentation and host animal nutrition through modulation of rumen microbiota. The goals have been decreasing methane production, partially inhibiting protein degradation to avoid excess release of ammonia, and activation of fiber digestion. The main approach has been the use of dietary supplements. Since growth-promoting antibiotics were banned in European countries in2006, safer alternatives including plant-derived materials have been explored. Plant oils, their component fatty acids,plant secondary metabolites and other compounds have been studied, and many originate or are abundantly available in Asia as agricultural byproducts. In this review, the potency of selected byproducts in inhibition of methane production and protein degradation, and in stimulation of fiber degradation was described in relation to their modes of action. In particular, cashew and ginkgo byproducts containing alkylphenols to mitigate methane emission and bean husks as a source of functional fiber to boost the number of fiber-degrading bacteria were highlighted. Other byproducts influencing rumen microbiota and fermentation profile were also described. Future application of these feed and additive candidates is very dependent on a sufficient, cost-effective supply and optimal usage in feeding practice. 展开更多
关键词 Agricultural byproduct Fermentation Fiber degradation Methane mitigation Microbiota Plant secondary metabolites Rumen
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In vitro Metabolomic Approaches to Investigating the Potential Biological Effects of Phenolic Compounds:An Update 被引量:1
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作者 Ursula Catalan Laura Barrubes +2 位作者 Rosa Maria Vails Rosa Soila Laura Rubio 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2017年第4期236-245,共10页
Dietary phenolic compounds (PCs) have been receiving intercst lor their presumcd roles ill disease prevention. However, there is a lack of studies on the underlying molecular mechanisnls. In this regard, in vitro me... Dietary phenolic compounds (PCs) have been receiving intercst lor their presumcd roles ill disease prevention. However, there is a lack of studies on the underlying molecular mechanisnls. In this regard, in vitro metabolomic approaches are suitable for the investigation o1 the molecular changes in response to PC exposure. Up to date, the biological effects of PCs have only been cxalnined for PCs from rosemary (Rosmarinus officinalis), olive oil, and reSVCl-atrol using cell-based mctabolomic approach, although transcriptomic and/or proteomic studies have also been conducted in the same in vi[ro cell experiment in some cases. Our integral analysis of the reviewed studies suggest that PCs may be involved not only in basic cellular processes or macro-and micro-nutrient meta- bolism, but also in specific metabolic pathways that have been thoroughly investigated. These modulated pathways could have a clinical impact on neurodegenerativc diseases, type 2 diabetes, cancer, and cardiovascular diseases. In conclusion, the in vitro metabolomic approaches provide additional information of the molecular mechanisms involved in disease risk reduction of dietary PCs. In order to elucidate the mechanisms of action of PCs, more metabolomic cell-based studies are nccdcd and testing the physiological conjugated forms of PCs in these cell systems could be of special interest. 展开更多
关键词 Cell-based Metabolomics Phenolic compounds Mechanism of action Disease prevention
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Litopenaeus vannamei immunestimulated with Macrocystis pyrifera extract:improving the immune response against Vibrio campbellii 被引量:1
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作者 Liliana Noemi Sánchez Campos Fernando Díaz Herrera +4 位作者 Ana Denisse Re Araujo Ricardo Alberto Gonzalez Sánchez Marcial Leonardo Lizárraga Partida Manuel de Jesús Acosta Ruiz Alexei Fedorovish Licea Navarro 《Journal of Coastal Life Medicine》 2014年第8期617-624,共8页
Objective:To examine the immune responses of Litopenaeus vannamei after different treatments with a hot water extract of Macrocystis pyrifera(M.pyrifera)and a subsequent challenge with Vibrio campbellii(V.campbellii).... Objective:To examine the immune responses of Litopenaeus vannamei after different treatments with a hot water extract of Macrocystis pyrifera(M.pyrifera)and a subsequent challenge with Vibrio campbellii(V.campbellii).Methods:A total of 184 adult white shrimp that were infected with V.campbellii(1×10^(6) CFU/shrimp)were immunostimulate by the hot-water extract from M.pyrifera via either injection(10μg)or immersion(350 mg/L),the experimental controls were injected with either saline solution or V.campbellii(1×10^(6) CFU/shrimp).The bacterial DNA depuration rate,antimicrobial activity and total hemocyte count were evaluated in hemolymph samples at 2,6,12,24,48 and 72 h post-infection.Results:Injected shrimp(10μg M.pyrifera extract)demonstrated the best clearance of bacterial infection,with 82%survival at 72 h post-infection(cellular response).Hemolymph from the immersed organisms had the best antimicrobial activity against Escherichia coli growth;specifically,the most efficient antimicrobial activity was observed at 24 h post-infection.Both types of immunostimulated shrimp had similar total hemocyte counts at 24 h post-infection(1.63-1.59 million/mL);however,after 72 h,injected shrimp had higher total hemocyte counts than immersed animals(2.59 v.s.0.56 million/mL).Conclusions:The injection of the M.pyrifera hot-water extract facilitated a more efficient response to V.campbellii infection due to the stimulation of the hemocytes of the shrimp.In other words,the cellular immune response was more efficient to eliminate bacterial infection than the humoral response in shrimp. 展开更多
关键词 Immune stimulation Hot-water extract Clearance rate Antimicrobial activity SEAWEED Vibrio campbellii Litopenaeus vannamei Macrocystis pyrifera PCR
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