Mistletoes are ecologically important parasitic plants,with> 1600 species from five lineages worldwide.Mistletoe lineages exhibit distinct patterns of species diversification and host specificity,however,the mechan...Mistletoes are ecologically important parasitic plants,with> 1600 species from five lineages worldwide.Mistletoe lineages exhibit distinct patterns of species diversification and host specificity,however,the mechanisms underlying these differences are poorly understood.In this study,we analysed a comprehensive parasite-host network,including 280 host species from 60 families and 22 mistletoe species from two lineages(Santalaceae and Loranthaceae) in Xishuangbanna,located in a biodiversity hotspot of tropical Asia.We identified the factors that predict the infection strength of mistletoes.We also detected host specificity and the phylogenetic signal of mistletoes and their hosts.We found that this interaction network could be largely explained by a model based on the relative abundance of species.Host infection was positively correlated with diameter at breast height and tree coverage,but negatively correlated with wood density.Overall,closely related mistletoe species tend to interact more often with similar hosts.However,the two lineages showed a significantly different network pattern.Rates of host generality were higher in Loranthaceae than in Santalaceae,although neither lineage showed phylogenetic signal for host generality.This study demonstrates that the neutral interaction hypothesis provides suitable predictions of the mistletoe-host interaction network,and mistletoe species show significant phylogenetic signals for their hosts.Our findings also indicate that high species diversification in Loranthaceae may be explained by high rates of host generality and the evolutionary history shared by Loranthaceae species with diverse host plants in the tropics.展开更多
Mauremys mutica(Cantor,1842)is an endangered species in China.Main phenotypic variations inbody color,body weight,body shape,clutch size,egg size,and hatchling size were revealed betweenthe southern and northern popul...Mauremys mutica(Cantor,1842)is an endangered species in China.Main phenotypic variations inbody color,body weight,body shape,clutch size,egg size,and hatchling size were revealed betweenthe southern and northern populations.Both populations have the phenomenon of'larger male'sexualsize dimorphism(SSD),especially in the southern population.Furthermore,genetic variations betweenthe two populations were analyzed by RAPD band patterns of 30 random individuals in each population.The average genetic distance was 0.299±0.108 among the samples of two populations.The average ge-netic distance between southern and northern populations was 0.305±0.046.Cluster analysis indicatedthat all the individuals from the southern and northern populations were clustered among themselves toform two distinct clades.A total of 20 population-specific RAPD fragments were scored from 16 primers,and could be used as RAPD markers for distinguishing the southern and the northern population.Basedon the nucleotide sequences of two RAPD markers,two pairs of SCAR primers(SC1-S and SC2-S)weredesigned,which could be used as SCAR markers for the southern population.According to the significantphenotypic and genetic variations,we suggested that the northern population and southern populationmight be considered as two separate taxa,the'northern taxon'and the'southern taxon',and the con-servation should be respectively conducted on the two taxa.展开更多
Extracts of 21 marine invertebrates belonging to Coelenterata, Mollusca, Annelida, Bryozoa, Echiura, Arthropoda, Echinodermata and Urochordata were screened for the studies on their antitumor and immune regulation act...Extracts of 21 marine invertebrates belonging to Coelenterata, Mollusca, Annelida, Bryozoa, Echiura, Arthropoda, Echinodermata and Urochordata were screened for the studies on their antitumor and immune regulation activities. Antitumor activity was determined by MTT method and immune regulation activity was studied using T- and B-lymphocytes in mice spleen in vitro. It was found that the n-butanol part of Asterina pectinifera, the acetic ether part of Tubuaria marina,95% ethanol extract of Acanthochiton rubrolineatus have a high inhibition rate of 96.7%,63.9% and 50.5% respectively on tumor cell line HL-60 at the concentration of 0.063 mg/ml. The inhibition rate of the acetic ether part of Tubuaria marina on the tumor cell line A-549 is 65.4 % at concentration of 0.063 mg/mL. The 95% ethanol extract of Meretrix meretrix has so outstanding promoting effect on T-lymphocytes that their multiplication increases 25% when the sample concentration is only 1 μg/ml. On B-lymphocytes, the 95% extract of Rapana venosa, at concentration of 100 μg/ml, has a promotion percent- age of 60%. On the other hand, under the condition of no cytotoxic effect, the 95% ethanol extracts of Acantho- chiton rubrolineatus and Cellana toreum can reach 92% inhibition rate on T lymphocyte at concentration of 100 μg/ml, while the inhibition rate on B lymphocyte of the 95% extract of Acanthochiton rubrolineatus reaches 92% at the same concentration.展开更多
On the interannual timescale, the meridional displacement of the East Asian upper-tropospheric jet stream (EAJS) is significantly associated with the rainfall anomalies in East Asia in summer. In this study, using the...On the interannual timescale, the meridional displacement of the East Asian upper-tropospheric jet stream (EAJS) is significantly associated with the rainfall anomalies in East Asia in summer. In this study, using the data from the National Centers for Environmental Prediction-Department of Energy (NCEP/DOE) reanalysis-2 from 1979 to 2002, the authors investigate the interannual variations of the EAJS's meridional displacement in summer and their associations with the variations of the South Asian high (SAH) and the western North Pacific subtropical high (WNPSH), which are dominant circulation features in the upper and lower troposhere, respectively. The result from an EOF analysis shows that the meridional displacement is the most remarkable feature of the interannual variations of the EAJS in each month of summer and in summer as a whole. A composite analysis indicates that the summer (June-JulyAugust, JJA) EAJS index, which is intended to depict the interannual meridional displacement of the EAJS, is not appropriate because the anomalies of the zonal wind at 200 hPa (U200) in July and August only, rather than in June, significantly contribute to the summer EAJS index. Thus, the index for each month in summer is defined according to the location of the EAJS core in each month. Composite analyses based on the monthly indexes show that corresponding to the monthly equatorward displacement of the EAJS, the South Asian high (SAH) extends southeastward clearly in July and August, and the western North Pacific subtropical high (WNPSH) withdraws southward in June and August.展开更多
Numerous studies on the aerodynamics of insect wing flapping were carried out on different approaches of flight investigations, model experiments, and numerical simulations, but the theoretical modeling remains to be ...Numerous studies on the aerodynamics of insect wing flapping were carried out on different approaches of flight investigations, model experiments, and numerical simulations, but the theoretical modeling remains to be explored. In the present paper, an analytic approach is presented to model the flow interactions of wing flapping in air for small insects with the surrounding flow fields being highly unsteady and highly viscous. The model of wing flapping is a 2-D flat plate, which makes plunging and pitching oscillations as well as quick rotations reversing its positions of leading and trailing edges, respectively, during stroke reversals. It contains three simplified aerodynamic assumptions:(i) unsteady potential flow; (ii) discrete vortices shed from both leading and trailing edges of the wing; (iii) Kutta conditions applied at both edges. Then the problem is reduced to the solution of the unsteady Laplace equation, by using distributed singularities, i.e., sources/sinks, and vortices in the field. To validate the present physical model and analytic method proposed via benchmark examples, two elemental motions in wing flapping and a case of whole flapping cycles are analyzed,and the predicted results agree well with available experimental and numerical data. This verifies that the present analytical approach may give qualitatively correct and quantitatively reasonable results.Furthermore, the total fluid-dynamic force in the present method can be decomposed into three parts:one due to the added inertial (or mass) effect, the other and the third due to the induction of vortices shed from the leading- and the trailing-edge and their images respectively, and this helps to reveal the flow control mechanisms in insect wing flapping.展开更多
Soil and water losses through erosion have been serious in the black soil region of Northeast China. Therefore, a sloping cultivated land in Songnen Plain was selected as a case study to: 1) determine the 137Cs refere...Soil and water losses through erosion have been serious in the black soil region of Northeast China. Therefore, a sloping cultivated land in Songnen Plain was selected as a case study to: 1) determine the 137Cs reference inventory in the study area; 2) calculate erosion and deposition rates of black soil on different slope locations; 3) conduct a sensitivity analysis of some model parameters; and 4) compare overall outputs using four different models. Three transects were set in the field with five slope locations for each transect, including summit, shoulder-slope, back-slope, foot-slope, and toe-slope. Field measurements and model simulation were used to estimate a bomb-derived 137Cs reference inventory in the study area. Soil erosion and deposition rates were estimated using four 137Cs models and percentage of 137Cs loss/gain. The 137Cs reference value in the study area was 2 232.8 Bq m-2 with 137Cs showing a clear topographic pattern, decreasing from the summit to shoulder-slope, then increasing again at the foot-slope and reaching a maximum at the toe-slope. Predicted soil redistribution rates for different slope locations varied. Among models, the Yang Model (YANG-M) overestimated erosion loss but underestimated deposition. However, the standard mass balance model (MBM1) gave predictions similar to a mass balance model incorporating soil movement by tillage (MBM2). Sensitivity analysis of the proportion factor 7 and distribution pattern of 137Cs in the surface layer demonstrated the impact of 137Cs enrichment on calculation of the soil erosion rate. Factors influencing the redistribution of fallout 137Cs in landscape should be fully considered as calculating soil redistribution rate using 137Cs technique.展开更多
Soil enzymes activities and microbial biomass have an important influence on nutrient cycling. The spatial distribution of soil enzymes activities and microbial biomass were examined along a latitudinal gradient in fa...Soil enzymes activities and microbial biomass have an important influence on nutrient cycling. The spatial distribution of soil enzymes activities and microbial biomass were examined along a latitudinal gradient in farmlands of Songliao Plain, Northeast China to assess the impact of climatic changes along the latitudinal transect on nutrient cycling in agroecosystems. Top soils (0-20 cm depth) were sampled in fields at 7 locations from north (Hailun) to south (Dashiqiao) in the end of October 2005 after maize harvest. The contents of total C, N, and P, C/N, available N, and available P increased with the latitude. The activities of invertase and acid phosphatase, microbial biomass (MB) C and N, and MBC/MBN were significantly correlated with latitude (P < 0.05, r2 = 0.198, 0.635, 0.558, 0.211 and 0.317, respectively), that is, increasing with the latitude. Significant positive correlations (P < 0.05) were observed between invertase activity and the total N and available P, and between acid phosphatase activity and the total C, C/N, available N, total P and available P. The urease, acid phosphatase, and dehydrogenase activities were significantly correlated with the soil pH and electrical conductivity (EC) (P < 0.05). MBC and MBN were positively correlated with the total C, C/N, and available P (P < 0.05). The MBC/MBN ratio was positively correlated with the total C, total N, C/N, and available N (P < 0.05). The spatial distribution of soil enzyme activities and microbial biomass resulted from the changes in soil properties such as soil organic matter, soil pH, and EC, partially owing to variations in temperature and rainfall along the latitudinal gradient.展开更多
Based upon the 1970 aero-photo topographic map,and TM/ETM satellite images taken in 1991 and 2000,the authors artificially interpreted boundaries of lake and glaciers in Nam Co Catchment,and quantified lake-glacier ar...Based upon the 1970 aero-photo topographic map,and TM/ETM satellite images taken in 1991 and 2000,the authors artificially interpreted boundaries of lake and glaciers in Nam Co Catchment,and quantified lake-glacier area variations in different stages by "inte-grated method" with the support of GIS. Results show that from 1970 to 2000,lake area in-creased from 1942.34 km2 to 1979.79 km2 at a rate of 1.27 km2/a,while glacier area de-creased from 167.62 km2 to 141.88 km2 at a rate of 0.86 km2/a. The increasing rate of lake in 1991–2000 was 1.76 km2/a that was faster than 1.03 km2/a in 1970–1991,while in the same period of time,the shrinking rates of glaciers were 0.97 km2/a and 0.80 km2/a respectively. Important factors,relevant to lake and glacier response to the climate,such as air tempera-ture,precipitation,potential evapotranspiration and their values in warm and cold seasons,were discussed. The result suggests that temperature increasing is the main reason for the accelerated melting of glaciers. Lake expansion is mainly induced by the increase of the gla-cier melting water,increase of precipitation and obvious decrease of potential evapotranspi-ration. Precipitation,evaporation and their linkages with lake enlargement on regional scale need to be thoroughly studied under the background of global warming and glacier retreating.展开更多
Fuzzy classification combined with spatial prediction was used to assess the state of soil pollution in the peri-urban Beijing area. Total concentrations of As, Cr, Cd, Hg, and Pb were determined in 220 topsoil sample...Fuzzy classification combined with spatial prediction was used to assess the state of soil pollution in the peri-urban Beijing area. Total concentrations of As, Cr, Cd, Hg, and Pb were determined in 220 topsoil samples (0-20 cm) collected using a grid design in a study area of 2600 km2. Heavy metal concentrations were grouped into three classes according to the optimum number of classes and fuzziness exponent using the fuzzy c-mean (FCM) algorithm. Membership values were interpolated using ordinary kriging. The polluted soils of the study area induced by the measured heavy metals were concentrated in the northwest corner and eastern part, especially the southeastern part close to the urban zone, whereas the soils free of pollution were mainly distributed in the southwestern part. The soils with potential risk of heavy metal pollution were located in isolated spots mainly in the northern part and southeastern corner of the study region. The FCM algorithm combined with geostatistical techniques, as compared to conventional single geostatistical kriging methods, could produce a prediction with a quantitative uncertainty evaluation and higher reliability. Successful prediction of soil pollution achieved with FCM algorithm in this study indicated that fuzzy set theory had great potential for use in other areas of soil science.展开更多
A three-dimensional model is used to investigate the mechanism of the South China Sea (SCS) winter counter-current (also known as the SCS Warm Current,or the SCSWC),which flows against the wind.The model can reproduce...A three-dimensional model is used to investigate the mechanism of the South China Sea (SCS) winter counter-current (also known as the SCS Warm Current,or the SCSWC),which flows against the wind.The model can reproduce the structure of the band-like currents over the northern shelf of the SCS,including the westward coastal current and slop current,and the SCSWC sandwiched in between.Sensitivity experiments are designed to understand the different roles of Ekman pumping of the SCSWC at different longitude.The results show that the Ekman pumping drives the SCSWC in the west segment.In the east,it is not the Ekman pumping but the intrusion of the Kuroshio that drives the SCSWC.展开更多
The source region of the Yellow River is located in the middle east of the Tibetan Plateau in northwest China. The total area is about 51,700 km2, mainly covered by grassland (79%), unused land (16%) and water (4%). T...The source region of the Yellow River is located in the middle east of the Tibetan Plateau in northwest China. The total area is about 51,700 km2, mainly covered by grassland (79%), unused land (16%) and water (4%). The increasing land utilization in this area has increased the risk of environmental degradation. The land use/cover data (1985 and 2000) provided by the Data Center of Resources and Environment in the Chinese Academy of Sciences were used to analyze the land cover change in the source region of the Yellow River. DEM (1:250,000) data, roads and settlement data were used to analyze the spatial characteristics of grasslands degradation. The ArcGIS 9 software was used to convert data types and do the overlay, reclassification and zonal statistic analysis. Results show that grassland degradation is the most important land cover change in the study area, which occupied 8.24% of the region’s total area. Human activities are the main causes of the grassland degradation in the source region of the Yellow River: 1) the degradation rate is higher on the sunny slope than on the shady slope; 2) the grassland degradation rate decreases with an increase in the elevation, and it has a correlation coefficient of -0.93; 3) the nearer to the settlements the grassland is, the higher the degradation rate. Especially within a distance range of 12 km to the settlements, the grassland degradation rate is highly related with the distance, with a coefficient of -0.99; and 4) in the range of 4 km, the degradation rate decreases with the increase of distance to the roads, with a correlation coefficient of -0.98. Besides some physical factors, human activities have been the most important driving forces of the grassland degradation in the source region of the Yellow River since 1985. To resolve the degradation problems, population control is essential, and therefore, it can reduce the social demand of livestock products from the grassland. To achieve sustainable development, it needs to improve the management of grassland ecosystem.展开更多
The influence of Cu and Zn on soil nematode communities was examined a long a pollution gradient with increasing distance from a metallurgical factory. Total and available heavy metal contents were used to study the e...The influence of Cu and Zn on soil nematode communities was examined a long a pollution gradient with increasing distance from a metallurgical factory. Total and available heavy metal contents were used to study the effects of heav y metals on nematode abundance, trophic groups and ecological indices. The resul ts demonstrated significant correlations between the number of total nematodes, bacterivores, plant-parasites and the total and available heavy metals. Bacteriv ores and plant-parasites were the dominant trophic groups. Significant differenc es in different sampling sites were found only in the number of bacterivores(P<0 .01). The Shannon-Weaver diversity index(H’), trophic diversity index(TD), evenn ess index(J ’) and dominance index(?姿) were found to be sensitive to soil pH an d C/N ratios. Significant correlations were found between the total nematodes(TN EM), some genera(Acrobeloides, Aphelenchoides, Cephalobus, Ditylenchus, Mesorhab ditis, Tetylenchus and Tylenchus) and distance from the factory.展开更多
Soil erosion in hilly areas of the Sichuan Basin is a serious concern over sustainable crop production and sound ecosystem. A 3-year experiment was conducted using the method of runoff plots to examine the effects of ...Soil erosion in hilly areas of the Sichuan Basin is a serious concern over sustainable crop production and sound ecosystem. A 3-year experiment was conducted using the method of runoff plots to examine the effects of terracing and agroforestry in farmland systems on soil and water conservation of slope fields in the hilly areas in Jianyang County, Sichuan Province, Southwestern China. A power function (Y = aXb) can statistically describe the relationship between water runoff (Y) and rainfall (X). The regression equation for the treatment of sloping terraces with crops (Plot 2) is remarkably different from that for the treatment of sloping terraces with grasses and trees (Plot 1) and the conventional up- and down-slope crop system (Plot 3) regarding equation coefficients, while regression equations are similar between Plot 1 and Plot 3. Water runoff amount and runoff coefficient of slope fields increased by 21.5～41.0 % and 27.5～69.7 % respectively, compared to those of sloping terraces, suggesting that terracing notably reduced the water runoff in the field. In the case of sloping terraces, lower amount of water runoff was observed on sloping terraces with crops than on sloping terraces with grasses and trees. Sediment yields on the slope fields in the normal year of rainfall distribution were notably higher (34.41～331.67 % and 37.06～403.44 % for Plot 1 and Plot 2, respectively) than those on sloping terraces, implying that terracing also plays a significant role in the reduction in soil erosion. It is suggested that terracing with crops is significantly effective for soil and water conservation in cultivatedfarmland, while the conventional practice of up- and down- slope cultivation creates high rates of water runoff and soil sediment transport. Terracing with grasses and fruit trees shows a less reduction in water runoff than terracing with crops, which was observed in the 3-year experiments.展开更多
Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil ni...Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination. Acetochlor reduced the nifH gene diversity (both in gene richness and diversity index values) and caused changes in the nifH gene composition. The effects of acetochlor on nifH gene were strengthened as the concentration of acetochlor increased. Cluster analysis of DGGE banding patterns showed that nifH gene composition which had been affected by low concentration of acetochlor (50 mgkg) recovered firstly. Methamidophos reduced nifH gene richness that except at 4 weeks. The medium concentration of methamidophos (150 mgkg) caused the most apparent changes in nifH gene diversity at the first week while the high concentration of methamidophos (250 mgkg) produced prominent effects on nifH gene diversity in the following weeks. Cluster analysis showed that minimal changes of nifH gene composition were found at 1 week and maximal changes at 4 weeks. Toxic effects of acetochlor and methamidophos combination on nifH gene were also apparent. Different nifH genes (bands) responded differently to the impact of agrochemicals: four individual bands were eliminated by the application of the agrochemicals, five bands became predominant by the stimulation of the agrochemicals, and four bands showed strong resistance to the inffuence of the agrochemicals. Fifteen prominent bands were partially sequenced, yielding 15 different nifH sequences, which were used for phylogenetic reconstructions. All sequences were affliated with the alpha-and beta-proteobacteria, showing higher similarity to eight different diazotrophic genera.展开更多
Three forests with different historical land-use, forest age, and spec ies assemblages in subtropical China were selected to evaluate current soil N st atus and investigate the responses of soil inorganic N dynamics t...Three forests with different historical land-use, forest age, and spec ies assemblages in subtropical China were selected to evaluate current soil N st atus and investigate the responses of soil inorganic N dynamics to monthly ammon ium nitrate additions. Results showed that the mature monsoon evergreen broadlea ved forest that has been protected for more than 400 years exhibited an advanced soil N status than the pine (Pinus massoniana) and pine-broadleaf mixed forests , both originated from the 1930’s clear-cut and pine plantation. Mature forests had greater extractable inorganic N pool, lower N retention capacity, higher in organic N leaching, and higher soil C/N ratios. Mineral soil extractable NH4+-N and NO3--N concentrations were significantly increased by experimental N additio ns on several sampling dates, but repeated ANOVA showed that the effect was not significant over the whole year except NH4+-N in the mature forest. In contrast, inorganic N (both NH4+-N and NO3--N) in soil 20-cm below the surface was signif icantly elevated by the N additions. From 42% to 74% of N added was retained by the upper 20 cm soils in the pine and mixed forests, while 0%—70% was retained in the mature forest. Our results suggest that land-use history, forest age and species composition were likely to be some of the important factors that determi ne differing forest N retention responses to elevated N deposition in the study region.展开更多
基金This study was financially supported by the National Natural Science Foundation of China(Grant No.31670393 and 32270310).
文摘Mistletoes are ecologically important parasitic plants,with> 1600 species from five lineages worldwide.Mistletoe lineages exhibit distinct patterns of species diversification and host specificity,however,the mechanisms underlying these differences are poorly understood.In this study,we analysed a comprehensive parasite-host network,including 280 host species from 60 families and 22 mistletoe species from two lineages(Santalaceae and Loranthaceae) in Xishuangbanna,located in a biodiversity hotspot of tropical Asia.We identified the factors that predict the infection strength of mistletoes.We also detected host specificity and the phylogenetic signal of mistletoes and their hosts.We found that this interaction network could be largely explained by a model based on the relative abundance of species.Host infection was positively correlated with diameter at breast height and tree coverage,but negatively correlated with wood density.Overall,closely related mistletoe species tend to interact more often with similar hosts.However,the two lineages showed a significantly different network pattern.Rates of host generality were higher in Loranthaceae than in Santalaceae,although neither lineage showed phylogenetic signal for host generality.This study demonstrates that the neutral interaction hypothesis provides suitable predictions of the mistletoe-host interaction network,and mistletoe species show significant phylogenetic signals for their hosts.Our findings also indicate that high species diversification in Loranthaceae may be explained by high rates of host generality and the evolutionary history shared by Loranthaceae species with diverse host plants in the tropics.
基金the National Basic Research Program of China(No.2004CB117405)the Key Research Program of Ocean and Fishery Bureau of Guangdong province(No.2001A09)
文摘Mauremys mutica(Cantor,1842)is an endangered species in China.Main phenotypic variations inbody color,body weight,body shape,clutch size,egg size,and hatchling size were revealed betweenthe southern and northern populations.Both populations have the phenomenon of'larger male'sexualsize dimorphism(SSD),especially in the southern population.Furthermore,genetic variations betweenthe two populations were analyzed by RAPD band patterns of 30 random individuals in each population.The average genetic distance was 0.299±0.108 among the samples of two populations.The average ge-netic distance between southern and northern populations was 0.305±0.046.Cluster analysis indicatedthat all the individuals from the southern and northern populations were clustered among themselves toform two distinct clades.A total of 20 population-specific RAPD fragments were scored from 16 primers,and could be used as RAPD markers for distinguishing the southern and the northern population.Basedon the nucleotide sequences of two RAPD markers,two pairs of SCAR primers(SC1-S and SC2-S)weredesigned,which could be used as SCAR markers for the southern population.According to the significantphenotypic and genetic variations,we suggested that the northern population and southern populationmight be considered as two separate taxa,the'northern taxon'and the'southern taxon',and the con-servation should be respectively conducted on the two taxa.
基金Supported by HTRDPC Projects (2001 AA620403, 503) and Key Innovative Project o the Chinese Academy of Sciences(KZCX3-SW-215)
文摘Extracts of 21 marine invertebrates belonging to Coelenterata, Mollusca, Annelida, Bryozoa, Echiura, Arthropoda, Echinodermata and Urochordata were screened for the studies on their antitumor and immune regulation activities. Antitumor activity was determined by MTT method and immune regulation activity was studied using T- and B-lymphocytes in mice spleen in vitro. It was found that the n-butanol part of Asterina pectinifera, the acetic ether part of Tubuaria marina,95% ethanol extract of Acanthochiton rubrolineatus have a high inhibition rate of 96.7%,63.9% and 50.5% respectively on tumor cell line HL-60 at the concentration of 0.063 mg/ml. The inhibition rate of the acetic ether part of Tubuaria marina on the tumor cell line A-549 is 65.4 % at concentration of 0.063 mg/mL. The 95% ethanol extract of Meretrix meretrix has so outstanding promoting effect on T-lymphocytes that their multiplication increases 25% when the sample concentration is only 1 μg/ml. On B-lymphocytes, the 95% extract of Rapana venosa, at concentration of 100 μg/ml, has a promotion percent- age of 60%. On the other hand, under the condition of no cytotoxic effect, the 95% ethanol extracts of Acantho- chiton rubrolineatus and Cellana toreum can reach 92% inhibition rate on T lymphocyte at concentration of 100 μg/ml, while the inhibition rate on B lymphocyte of the 95% extract of Acanthochiton rubrolineatus reaches 92% at the same concentration.
基金This work was supported by the Chinese Academy of Sciences(Grant No.KZCX3 SW-221)the National Natural Science Foundation of China under Grant No.40221503.
文摘On the interannual timescale, the meridional displacement of the East Asian upper-tropospheric jet stream (EAJS) is significantly associated with the rainfall anomalies in East Asia in summer. In this study, using the data from the National Centers for Environmental Prediction-Department of Energy (NCEP/DOE) reanalysis-2 from 1979 to 2002, the authors investigate the interannual variations of the EAJS's meridional displacement in summer and their associations with the variations of the South Asian high (SAH) and the western North Pacific subtropical high (WNPSH), which are dominant circulation features in the upper and lower troposhere, respectively. The result from an EOF analysis shows that the meridional displacement is the most remarkable feature of the interannual variations of the EAJS in each month of summer and in summer as a whole. A composite analysis indicates that the summer (June-JulyAugust, JJA) EAJS index, which is intended to depict the interannual meridional displacement of the EAJS, is not appropriate because the anomalies of the zonal wind at 200 hPa (U200) in July and August only, rather than in June, significantly contribute to the summer EAJS index. Thus, the index for each month in summer is defined according to the location of the EAJS core in each month. Composite analyses based on the monthly indexes show that corresponding to the monthly equatorward displacement of the EAJS, the South Asian high (SAH) extends southeastward clearly in July and August, and the western North Pacific subtropical high (WNPSH) withdraws southward in June and August.
文摘Numerous studies on the aerodynamics of insect wing flapping were carried out on different approaches of flight investigations, model experiments, and numerical simulations, but the theoretical modeling remains to be explored. In the present paper, an analytic approach is presented to model the flow interactions of wing flapping in air for small insects with the surrounding flow fields being highly unsteady and highly viscous. The model of wing flapping is a 2-D flat plate, which makes plunging and pitching oscillations as well as quick rotations reversing its positions of leading and trailing edges, respectively, during stroke reversals. It contains three simplified aerodynamic assumptions:(i) unsteady potential flow; (ii) discrete vortices shed from both leading and trailing edges of the wing; (iii) Kutta conditions applied at both edges. Then the problem is reduced to the solution of the unsteady Laplace equation, by using distributed singularities, i.e., sources/sinks, and vortices in the field. To validate the present physical model and analytic method proposed via benchmark examples, two elemental motions in wing flapping and a case of whole flapping cycles are analyzed,and the predicted results agree well with available experimental and numerical data. This verifies that the present analytical approach may give qualitatively correct and quantitatively reasonable results.Furthermore, the total fluid-dynamic force in the present method can be decomposed into three parts:one due to the added inertial (or mass) effect, the other and the third due to the induction of vortices shed from the leading- and the trailing-edge and their images respectively, and this helps to reveal the flow control mechanisms in insect wing flapping.
基金Project supported by the Hundred Talents Program of Chinese Academy of Sciences (No. K09Z3) the National Natural Science Foundation of China (Nos. 40271108 and 40471125).
文摘Soil and water losses through erosion have been serious in the black soil region of Northeast China. Therefore, a sloping cultivated land in Songnen Plain was selected as a case study to: 1) determine the 137Cs reference inventory in the study area; 2) calculate erosion and deposition rates of black soil on different slope locations; 3) conduct a sensitivity analysis of some model parameters; and 4) compare overall outputs using four different models. Three transects were set in the field with five slope locations for each transect, including summit, shoulder-slope, back-slope, foot-slope, and toe-slope. Field measurements and model simulation were used to estimate a bomb-derived 137Cs reference inventory in the study area. Soil erosion and deposition rates were estimated using four 137Cs models and percentage of 137Cs loss/gain. The 137Cs reference value in the study area was 2 232.8 Bq m-2 with 137Cs showing a clear topographic pattern, decreasing from the summit to shoulder-slope, then increasing again at the foot-slope and reaching a maximum at the toe-slope. Predicted soil redistribution rates for different slope locations varied. Among models, the Yang Model (YANG-M) overestimated erosion loss but underestimated deposition. However, the standard mass balance model (MBM1) gave predictions similar to a mass balance model incorporating soil movement by tillage (MBM2). Sensitivity analysis of the proportion factor 7 and distribution pattern of 137Cs in the surface layer demonstrated the impact of 137Cs enrichment on calculation of the soil erosion rate. Factors influencing the redistribution of fallout 137Cs in landscape should be fully considered as calculating soil redistribution rate using 137Cs technique.
基金the National Key Basic Research Support Foundation of China (No.2005CB121105)theNational Natural Science Foundation of China (No.30670379).
文摘Soil enzymes activities and microbial biomass have an important influence on nutrient cycling. The spatial distribution of soil enzymes activities and microbial biomass were examined along a latitudinal gradient in farmlands of Songliao Plain, Northeast China to assess the impact of climatic changes along the latitudinal transect on nutrient cycling in agroecosystems. Top soils (0-20 cm depth) were sampled in fields at 7 locations from north (Hailun) to south (Dashiqiao) in the end of October 2005 after maize harvest. The contents of total C, N, and P, C/N, available N, and available P increased with the latitude. The activities of invertase and acid phosphatase, microbial biomass (MB) C and N, and MBC/MBN were significantly correlated with latitude (P < 0.05, r2 = 0.198, 0.635, 0.558, 0.211 and 0.317, respectively), that is, increasing with the latitude. Significant positive correlations (P < 0.05) were observed between invertase activity and the total N and available P, and between acid phosphatase activity and the total C, C/N, available N, total P and available P. The urease, acid phosphatase, and dehydrogenase activities were significantly correlated with the soil pH and electrical conductivity (EC) (P < 0.05). MBC and MBN were positively correlated with the total C, C/N, and available P (P < 0.05). The MBC/MBN ratio was positively correlated with the total C, total N, C/N, and available N (P < 0.05). The spatial distribution of soil enzyme activities and microbial biomass resulted from the changes in soil properties such as soil organic matter, soil pH, and EC, partially owing to variations in temperature and rainfall along the latitudinal gradient.
基金National Basic Research Program of China, No.2005CB422002 National Natural Science Foundation of China, No.40331006+2 种基金 No.40571172 Knowledge Innovation Project of the CAS, No.KZCX3-SW-339 The authors would like to thank the National Climatic Data Center of China Meteorological Administration （CMA） for providing climatic data for this study.
文摘Based upon the 1970 aero-photo topographic map,and TM/ETM satellite images taken in 1991 and 2000,the authors artificially interpreted boundaries of lake and glaciers in Nam Co Catchment,and quantified lake-glacier area variations in different stages by "inte-grated method" with the support of GIS. Results show that from 1970 to 2000,lake area in-creased from 1942.34 km2 to 1979.79 km2 at a rate of 1.27 km2/a,while glacier area de-creased from 167.62 km2 to 141.88 km2 at a rate of 0.86 km2/a. The increasing rate of lake in 1991–2000 was 1.76 km2/a that was faster than 1.03 km2/a in 1970–1991,while in the same period of time,the shrinking rates of glaciers were 0.97 km2/a and 0.80 km2/a respectively. Important factors,relevant to lake and glacier response to the climate,such as air tempera-ture,precipitation,potential evapotranspiration and their values in warm and cold seasons,were discussed. The result suggests that temperature increasing is the main reason for the accelerated melting of glaciers. Lake expansion is mainly induced by the increase of the gla-cier melting water,increase of precipitation and obvious decrease of potential evapotranspi-ration. Precipitation,evaporation and their linkages with lake enlargement on regional scale need to be thoroughly studied under the background of global warming and glacier retreating.
基金Project supported by the National Natural Science Foundation of China (Nos. 40571065 and 40235054)the National Key Basic Research Support Foundation of China (No. G1999045707).
文摘Fuzzy classification combined with spatial prediction was used to assess the state of soil pollution in the peri-urban Beijing area. Total concentrations of As, Cr, Cd, Hg, and Pb were determined in 220 topsoil samples (0-20 cm) collected using a grid design in a study area of 2600 km2. Heavy metal concentrations were grouped into three classes according to the optimum number of classes and fuzziness exponent using the fuzzy c-mean (FCM) algorithm. Membership values were interpolated using ordinary kriging. The polluted soils of the study area induced by the measured heavy metals were concentrated in the northwest corner and eastern part, especially the southeastern part close to the urban zone, whereas the soils free of pollution were mainly distributed in the southwestern part. The soils with potential risk of heavy metal pollution were located in isolated spots mainly in the northern part and southeastern corner of the study region. The FCM algorithm combined with geostatistical techniques, as compared to conventional single geostatistical kriging methods, could produce a prediction with a quantitative uncertainty evaluation and higher reliability. Successful prediction of soil pollution achieved with FCM algorithm in this study indicated that fuzzy set theory had great potential for use in other areas of soil science.
基金The Knowledge Innovation Program of the Chinese Academy of Sciences under contract No.KZCX1-YW-12-01the National Science Fund for Distinguished Young Scholars under contract No.40625017+1 种基金the National Natural Science Foundation of China(Key Program)under contract No.40830851the National Program on Key Basic Research Project(973Program)under contract No.2007CB816004
文摘A three-dimensional model is used to investigate the mechanism of the South China Sea (SCS) winter counter-current (also known as the SCS Warm Current,or the SCSWC),which flows against the wind.The model can reproduce the structure of the band-like currents over the northern shelf of the SCS,including the westward coastal current and slop current,and the SCSWC sandwiched in between.Sensitivity experiments are designed to understand the different roles of Ekman pumping of the SCSWC at different longitude.The results show that the Ekman pumping drives the SCSWC in the west segment.In the east,it is not the Ekman pumping but the intrusion of the Kuroshio that drives the SCSWC.
基金National Natural Science Foundation of China, No.90202012 National Basic Research Program of China, No.2005CB422006+1 种基金 No.2002CB412507 Knowledge Innovation Project of CAS, No.KZCX3-SW-339
文摘The source region of the Yellow River is located in the middle east of the Tibetan Plateau in northwest China. The total area is about 51,700 km2, mainly covered by grassland (79%), unused land (16%) and water (4%). The increasing land utilization in this area has increased the risk of environmental degradation. The land use/cover data (1985 and 2000) provided by the Data Center of Resources and Environment in the Chinese Academy of Sciences were used to analyze the land cover change in the source region of the Yellow River. DEM (1:250,000) data, roads and settlement data were used to analyze the spatial characteristics of grasslands degradation. The ArcGIS 9 software was used to convert data types and do the overlay, reclassification and zonal statistic analysis. Results show that grassland degradation is the most important land cover change in the study area, which occupied 8.24% of the region’s total area. Human activities are the main causes of the grassland degradation in the source region of the Yellow River: 1) the degradation rate is higher on the sunny slope than on the shady slope; 2) the grassland degradation rate decreases with an increase in the elevation, and it has a correlation coefficient of -0.93; 3) the nearer to the settlements the grassland is, the higher the degradation rate. Especially within a distance range of 12 km to the settlements, the grassland degradation rate is highly related with the distance, with a coefficient of -0.99; and 4) in the range of 4 km, the degradation rate decreases with the increase of distance to the roads, with a correlation coefficient of -0.98. Besides some physical factors, human activities have been the most important driving forces of the grassland degradation in the source region of the Yellow River since 1985. To resolve the degradation problems, population control is essential, and therefore, it can reduce the social demand of livestock products from the grassland. To achieve sustainable development, it needs to improve the management of grassland ecosystem.
文摘The influence of Cu and Zn on soil nematode communities was examined a long a pollution gradient with increasing distance from a metallurgical factory. Total and available heavy metal contents were used to study the effects of heav y metals on nematode abundance, trophic groups and ecological indices. The resul ts demonstrated significant correlations between the number of total nematodes, bacterivores, plant-parasites and the total and available heavy metals. Bacteriv ores and plant-parasites were the dominant trophic groups. Significant differenc es in different sampling sites were found only in the number of bacterivores(P<0 .01). The Shannon-Weaver diversity index(H’), trophic diversity index(TD), evenn ess index(J ’) and dominance index(?姿) were found to be sensitive to soil pH an d C/N ratios. Significant correlations were found between the total nematodes(TN EM), some genera(Acrobeloides, Aphelenchoides, Cephalobus, Ditylenchus, Mesorhab ditis, Tetylenchus and Tylenchus) and distance from the factory.
基金Project supported by the Knowledge Innovation Engineering Project of the Chinese Academy of Sciences(No. KSCX2-YW-N-46-06)the National Natural Science Foundation of China(No. 40501030).
基金the research funding provided by the National Basic Research Program (973 Program No. 2007CB407206) of Chinathe National Natural Science Foundation of China (40771027)the National Supporting Plan of Science and Technology of China (2008BAD98B04)
文摘Soil erosion in hilly areas of the Sichuan Basin is a serious concern over sustainable crop production and sound ecosystem. A 3-year experiment was conducted using the method of runoff plots to examine the effects of terracing and agroforestry in farmland systems on soil and water conservation of slope fields in the hilly areas in Jianyang County, Sichuan Province, Southwestern China. A power function (Y = aXb) can statistically describe the relationship between water runoff (Y) and rainfall (X). The regression equation for the treatment of sloping terraces with crops (Plot 2) is remarkably different from that for the treatment of sloping terraces with grasses and trees (Plot 1) and the conventional up- and down-slope crop system (Plot 3) regarding equation coefficients, while regression equations are similar between Plot 1 and Plot 3. Water runoff amount and runoff coefficient of slope fields increased by 21.5～41.0 % and 27.5～69.7 % respectively, compared to those of sloping terraces, suggesting that terracing notably reduced the water runoff in the field. In the case of sloping terraces, lower amount of water runoff was observed on sloping terraces with crops than on sloping terraces with grasses and trees. Sediment yields on the slope fields in the normal year of rainfall distribution were notably higher (34.41～331.67 % and 37.06～403.44 % for Plot 1 and Plot 2, respectively) than those on sloping terraces, implying that terracing also plays a significant role in the reduction in soil erosion. It is suggested that terracing with crops is significantly effective for soil and water conservation in cultivatedfarmland, while the conventional practice of up- and down- slope cultivation creates high rates of water runoff and soil sediment transport. Terracing with grasses and fruit trees shows a less reduction in water runoff than terracing with crops, which was observed in the 3-year experiments.
基金This work was supported by the National Basic Research Program(973)of China(No.2004CB418503)the National Natural Science Foundation of China(No.20477049).
文摘用集中坡度和联合污染物质暴露方法，单身者和关节在 polychaete Perinereis aibuhitensis Grube 上石油烃(PHC ) 和镉(Cd ) 完成，一生态地在河口的重点种类和沿海的环境，被调查了。结果显示到 P 的 PHC 的毒性。aibuhitensis 比对有机体的 Cd 的强壮。在答案在蠕虫的死亡和单个 Cd 或 PHC 的暴露的集中之间有正相关。同样，在蠕虫的 Cd 或 PHC 的累积与增加暴露 Cd 或暴露 PHC 的集中增加了。所有关联关系能用单一的二次的方程(Y 或 Z = aX2 + bX + c ) 被描述。它被计算，根据这些表情， P 的中部的致死剂量(LC50 ) 。暴露于单个 Cd 或 PHC 的 aibuhitensis 分别地是 793.4 13567.3 和 28.0 119.9 μ g /L。暴露的时间在二种污染物质上并且在蠕虫的死亡上有某刺激效果。因此，当暴露的时间变得更长时，甚至单个 Cd 或 PHC 的低集中可以在蠕虫上有强壮的有毒的效果。在蠕虫的 Cd 或 PHC 的累积在单个 Cd 或 PHC 的给定的暴露的集中随暴露时间的增加不同。显著地，在蠕虫的 PHC 的累积在答案在 PHC 的给定的高集中随暴露时间的增加减少。P 上的 PHC 和 Cd 的联合效果。aibuhitensis 是很复杂的并且随着二种污染物质的暴露的集中变化。在 PHC 的给定的集中，蠕虫上的二种污染物质的联合毒性随 Cd 集中的增加从协同改变到对抗。在蠕虫的 Cd 的累积显著地与暴露答案的 PHC 的增加减少。
基金Preject supported by the National Basic Research Program (973) of China(No. 2004CB418503)the Overseas Fund of Institute of AppliedEcology, Chinese Academy of Sciences.
文摘Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination. Acetochlor reduced the nifH gene diversity (both in gene richness and diversity index values) and caused changes in the nifH gene composition. The effects of acetochlor on nifH gene were strengthened as the concentration of acetochlor increased. Cluster analysis of DGGE banding patterns showed that nifH gene composition which had been affected by low concentration of acetochlor (50 mgkg) recovered firstly. Methamidophos reduced nifH gene richness that except at 4 weeks. The medium concentration of methamidophos (150 mgkg) caused the most apparent changes in nifH gene diversity at the first week while the high concentration of methamidophos (250 mgkg) produced prominent effects on nifH gene diversity in the following weeks. Cluster analysis showed that minimal changes of nifH gene composition were found at 1 week and maximal changes at 4 weeks. Toxic effects of acetochlor and methamidophos combination on nifH gene were also apparent. Different nifH genes (bands) responded differently to the impact of agrochemicals: four individual bands were eliminated by the application of the agrochemicals, five bands became predominant by the stimulation of the agrochemicals, and four bands showed strong resistance to the inffuence of the agrochemicals. Fifteen prominent bands were partially sequenced, yielding 15 different nifH sequences, which were used for phylogenetic reconstructions. All sequences were affliated with the alpha-and beta-proteobacteria, showing higher similarity to eight different diazotrophic genera.
文摘Three forests with different historical land-use, forest age, and spec ies assemblages in subtropical China were selected to evaluate current soil N st atus and investigate the responses of soil inorganic N dynamics to monthly ammon ium nitrate additions. Results showed that the mature monsoon evergreen broadlea ved forest that has been protected for more than 400 years exhibited an advanced soil N status than the pine (Pinus massoniana) and pine-broadleaf mixed forests , both originated from the 1930’s clear-cut and pine plantation. Mature forests had greater extractable inorganic N pool, lower N retention capacity, higher in organic N leaching, and higher soil C/N ratios. Mineral soil extractable NH4+-N and NO3--N concentrations were significantly increased by experimental N additio ns on several sampling dates, but repeated ANOVA showed that the effect was not significant over the whole year except NH4+-N in the mature forest. In contrast, inorganic N (both NH4+-N and NO3--N) in soil 20-cm below the surface was signif icantly elevated by the N additions. From 42% to 74% of N added was retained by the upper 20 cm soils in the pine and mixed forests, while 0%—70% was retained in the mature forest. Our results suggest that land-use history, forest age and species composition were likely to be some of the important factors that determi ne differing forest N retention responses to elevated N deposition in the study region.
