Totally 150 individuals of 1-day-old Guangxi Jinling partridges were randomly divided into five groups, 30 chickens each group. Chickens in control group were fed with basal diet, while chickens in experimental groups...Totally 150 individuals of 1-day-old Guangxi Jinling partridges were randomly divided into five groups, 30 chickens each group. Chickens in control group were fed with basal diet, while chickens in experimental groups were fed with the diet containing antibiotics, Chinese herbal medicine, probiotics and acidifi- er. The entire trial lasted 50 d, and growth performance and apparent nutrient digestibility of tested chickens were observed and measured. Compared with control group, the feed gain ratio in experimental groups significantly decreased （P 〈 0.05 ）, while that in probiotics group was the lowest. The average daily gain in pmbi- otics group was significantly higher than that in control group or other experimental groups （P 〈 0.05）. The apparent digestibility of crude protein, crude fat and energy in probiotics group were significantly higher than that in control group or other experimental groups （P 〈 0.05 ）. The apparent digestibility of phosphorus, crude fat, crude fiber, calcium and crude protein in antibiotics group were significantly higher than that in control group （P 〈 0.05 ）. The apparent digestibility of crude protein, crude fiber and phosphorus in Chinese herbal medicine group were significantly higher than that in control group （P 〈 0.05 ）. The apparent digesti- bility of nutrient in acidifier group were significantly lower than that in control group or other experimental groups （ P 〈 0.05 ）. Therefore, pmbiotics had better effects on growth performance and apparent nutrient digestibility of chickens than antibiotics, while Chinese herbal medicine and acidifier had inferior effect than an- tibiotics.展开更多
[ Objective] To observe sub-chronic toxicity of Tephrosia canadida DC. in mice and thus to evaluate their safety to be used as protein feed resources. [ Methed]Sixty-four 6-week-old healthy Kunming mice weighing about...[ Objective] To observe sub-chronic toxicity of Tephrosia canadida DC. in mice and thus to evaluate their safety to be used as protein feed resources. [ Methed]Sixty-four 6-week-old healthy Kunming mice weighing about 25 g were randomly divided into four groups （ n = 16）, half male and half female. Mass ratios of basic diet to leaf meal of Tephrosia canadida DC. in group A, group B, group C and group D were 10：0, 4：1, 3：2 and 2：3, respectively. After 35-d feeding, the effects of Tephrosia canadida DC. on growth, blood and organs of mice were observed. [ Resultl Dudng the trial, all mice had normal activities, and no death and no abnormal blood index were observed. All organs of the mice in the experimental groups had no visible pathological lesion, and organ indexes had no significant difference between the experimental groups and the control group. Except that slightly abnormal histological changes appeared in liver and kidney of the mice in the group C and D, no histological change was ob- served in other organs of the experimental mice. [ Conclusion] Tephrosia canadida DC. have no adverse effects on mice, which provides a refer- ence for research about their safety in feed of other animals.展开更多
To explore the feeding value of Pennisetum purpureum Schum cv. Guiminyin in production of geese, effects of feeding the green fodder on growth performance of geese were studied, while P. purpureum K. Scbumacb x P. typ...To explore the feeding value of Pennisetum purpureum Schum cv. Guiminyin in production of geese, effects of feeding the green fodder on growth performance of geese were studied, while P. purpureum K. Scbumacb x P. typhoideum Rich cv. Reyan No. 4 was used as control. The results showed that P. purpureum Schum cv. Guiminyin had high biomass and good nutritional quality, and geese intake of the forage was greater than that of the control variety. The average daily gain was （51.45 ±3.49）g/goose, which was increased by 13.36% compared with control; feed gain ratio was （4.93 ±0.35）%, and feed consumption was reduced by 19.47% compared with control （P 〈0.05）. Thus, it could be concluded that feeding geese with P. purpureum Schum ev. Guiminyin could obtain good economic benefit.展开更多
Fungi play a significant role in biology-related domains, and with the molecular biology technology advancing, identification of fungi at molecular level and verification of genetic transformant has become the necessa...Fungi play a significant role in biology-related domains, and with the molecular biology technology advancing, identification of fungi at molecular level and verification of genetic transformant has become the necessary step of test. By far, however, there is no ideal high-throughput molecular identification method. In this paper, a high-throughput device was designed, and a novel PCR-mediated molecular identification method suitable for the device was developed. Through cloning of ccllulase encoding genes and regulatory genes on the genome of Trichoderma reesei, cloning of promoter of phesphoglycerate kinase gene 1 and xylanase encoding genes on the genome and expression vector of Saccharomyces cerevisiae, and ITS sequences cloning and RAPD analysis of T. reesei, S. cerevisiae, Penicil- lium oxalicum, Rhizopus stolonifer, Aspergillus niger, A. carbonarius and A. japonicas, the method turned out to be an effective one with wide application potential. The establishment of the method has worked out the bottleneck of high-throughput molecular identification.展开更多
基金Supported by Program for Science and Technology Development of Guangxi Province(1123007-1)China Agricultural Industry Research System"Special Project for Guangxi Broiler Industry Innovation Team"(nycytxgxcxtd-04-20)
文摘Totally 150 individuals of 1-day-old Guangxi Jinling partridges were randomly divided into five groups, 30 chickens each group. Chickens in control group were fed with basal diet, while chickens in experimental groups were fed with the diet containing antibiotics, Chinese herbal medicine, probiotics and acidifi- er. The entire trial lasted 50 d, and growth performance and apparent nutrient digestibility of tested chickens were observed and measured. Compared with control group, the feed gain ratio in experimental groups significantly decreased （P 〈 0.05 ）, while that in probiotics group was the lowest. The average daily gain in pmbi- otics group was significantly higher than that in control group or other experimental groups （P 〈 0.05）. The apparent digestibility of crude protein, crude fat and energy in probiotics group were significantly higher than that in control group or other experimental groups （P 〈 0.05 ）. The apparent digestibility of phosphorus, crude fat, crude fiber, calcium and crude protein in antibiotics group were significantly higher than that in control group （P 〈 0.05 ）. The apparent digestibility of crude protein, crude fiber and phosphorus in Chinese herbal medicine group were significantly higher than that in control group （P 〈 0.05 ）. The apparent digesti- bility of nutrient in acidifier group were significantly lower than that in control group or other experimental groups （ P 〈 0.05 ）. Therefore, pmbiotics had better effects on growth performance and apparent nutrient digestibility of chickens than antibiotics, while Chinese herbal medicine and acidifier had inferior effect than an- tibiotics.
文摘[ Objective] To observe sub-chronic toxicity of Tephrosia canadida DC. in mice and thus to evaluate their safety to be used as protein feed resources. [ Methed]Sixty-four 6-week-old healthy Kunming mice weighing about 25 g were randomly divided into four groups （ n = 16）, half male and half female. Mass ratios of basic diet to leaf meal of Tephrosia canadida DC. in group A, group B, group C and group D were 10：0, 4：1, 3：2 and 2：3, respectively. After 35-d feeding, the effects of Tephrosia canadida DC. on growth, blood and organs of mice were observed. [ Resultl Dudng the trial, all mice had normal activities, and no death and no abnormal blood index were observed. All organs of the mice in the experimental groups had no visible pathological lesion, and organ indexes had no significant difference between the experimental groups and the control group. Except that slightly abnormal histological changes appeared in liver and kidney of the mice in the group C and D, no histological change was ob- served in other organs of the experimental mice. [ Conclusion] Tephrosia canadida DC. have no adverse effects on mice, which provides a refer- ence for research about their safety in feed of other animals.
基金Supported by National Science and Technology Project(2011BAD17B00)Science and Technology Project of Guangxi Provincial Bureau(GYMK201633019)+1 种基金Fund of Distinguished Experts in Guangxi Province&Guangxi Engineering Technology Center of Grassland Science(2015GCZX014)National Modern Agricultural Industry Technology System of Guangxi Beef Cattle,Mutton Sheep Industry Innovation Team(nycytxgxcxtd-09-04)
文摘To explore the feeding value of Pennisetum purpureum Schum cv. Guiminyin in production of geese, effects of feeding the green fodder on growth performance of geese were studied, while P. purpureum K. Scbumacb x P. typhoideum Rich cv. Reyan No. 4 was used as control. The results showed that P. purpureum Schum cv. Guiminyin had high biomass and good nutritional quality, and geese intake of the forage was greater than that of the control variety. The average daily gain was （51.45 ±3.49）g/goose, which was increased by 13.36% compared with control; feed gain ratio was （4.93 ±0.35）%, and feed consumption was reduced by 19.47% compared with control （P 〈0.05）. Thus, it could be concluded that feeding geese with P. purpureum Schum ev. Guiminyin could obtain good economic benefit.
基金Supported by Applied Fundamental Research Project of Guangxi Department of Science and Technology(14125008-2-13)
文摘Fungi play a significant role in biology-related domains, and with the molecular biology technology advancing, identification of fungi at molecular level and verification of genetic transformant has become the necessary step of test. By far, however, there is no ideal high-throughput molecular identification method. In this paper, a high-throughput device was designed, and a novel PCR-mediated molecular identification method suitable for the device was developed. Through cloning of ccllulase encoding genes and regulatory genes on the genome of Trichoderma reesei, cloning of promoter of phesphoglycerate kinase gene 1 and xylanase encoding genes on the genome and expression vector of Saccharomyces cerevisiae, and ITS sequences cloning and RAPD analysis of T. reesei, S. cerevisiae, Penicil- lium oxalicum, Rhizopus stolonifer, Aspergillus niger, A. carbonarius and A. japonicas, the method turned out to be an effective one with wide application potential. The establishment of the method has worked out the bottleneck of high-throughput molecular identification.
