Having been engaged in teaching, clinical practice and research for many years, Dr. Lai Xinsheng is good at acupuncture and the combined acupuncture and medication therapy to treat refractory diseases with satisfactor...Having been engaged in teaching, clinical practice and research for many years, Dr. Lai Xinsheng is good at acupuncture and the combined acupuncture and medication therapy to treat refractory diseases with satisfactory therapeutic effect. Sample cases treated by Dr. Lai are selected as follows.展开更多
BACKGROUND Accelerated therapeutic treatment should be considered in patients with progressive Crohn’s disease(CD)to prevent complications as well as surgery.Therefore,screening for risk factors and predicting the ne...BACKGROUND Accelerated therapeutic treatment should be considered in patients with progressive Crohn’s disease(CD)to prevent complications as well as surgery.Therefore,screening for risk factors and predicting the need for early surgery are of great importance in clinical practice.AIM To establish a model to predict CD-related early surgery.METHODS This was a retrospective study collecting data from CD patients diagnosed at our inflammatory bowel disease center from January 1,2012 to December 31,2016.All data were randomly stratified into a training set and a testing set at a ratio of 8:2.Multivariable logistic regression analysis was conducted with receiver operating characteristic curves constructed and areas under the curve calculated.This model was further validated with calibration and discrimination estimated.A nomogram was finally developed.RESULTS A total of 1002 eligible patients were enrolled with a mean follow-up period of 53.54±13.10 mo.In total,24.25%of patients received intestinal surgery within 1 year after diagnosis due to complications or disease relapse.Disease behavior(B2:OR[odds ratio]=6.693,P<0.001;B3:OR=14.405,P<0.001),smoking(OR=4.135,P<0.001),body mass index(OR=0.873,P<0.001)and C-reactive protein(OR=1.022,P=0.001)at diagnosis,previous perianal(OR=9.483,P<0.001)or intestinal surgery(OR=8.887,P<0.001),maximum bowel wall thickness(OR=1.965,P<0.001),use of biologics(OR=0.264,P<0.001),and exclusive enteral nutrition(OR=0.089,P<0.001)were identified as independent significant factors associated with early intestinal surgery.A prognostic model was established and further validated.The receiver operating characteristic curves and calculated areas under the curves(94.7%)confirmed an ideal predictive ability of this model with a sensitivity of 75.92%and specificity of 95.81%.A nomogram was developed to simplify the use of the predictive model in clinical practice.CONCLUSION This prognostic model can effectively predict 1-year risk of CD-related intestinal surgery,which will assist in screening progressive CD patients and tailoring therapeutic management.展开更多
Objective: To study the trauma and stress level of the pelvic fracture treated by 3D printing combined with lateral rectus abdominis approach surgery. Methods: A total of 50 patients with pelvic fractures who were tre...Objective: To study the trauma and stress level of the pelvic fracture treated by 3D printing combined with lateral rectus abdominis approach surgery. Methods: A total of 50 patients with pelvic fractures who were treated in the hospital between July 2015 and Jun 2017 were collected anddivided into control group (n=25) and observation group (n=25) by random number table method. Control group underwent lateral rectus abdominis approach surgery, and the observation group received 3D printingcombined with lateral rectus abdominis approach surgery. The differences in perioperative inflammatory response, coagulation function and stress degree were compared between the two groups. Results: 24 h before surgery, the differences in inflammatory response, coagulation function and stress degree were not statistically significant between the two groups. 24 h after surgery, serum IL-1, TNF-α, CRP, FIB, D-D, Cor, AngⅡ and NE levels of both groups of patients were higher than those 24h before surgery, and serum IL-1, TNF-α, CRP, FIB, D-D, Cor, AngⅡ and NE levels of observation group were lower than those of control group. Conclusion: 3D printing combined with lateral rectus abdominis approach surgery can effectively reduce the degree of surgical trauma and reduce the systemic stress response in patients with pelvic fracture.展开更多
To investigate the effect and mechanism of herbal medicine 960 recipe(960) on reversing the malignant phenotypes of human hepatocellular car-cinoma(HCC) SMMC7721 cells.Methods SMMC7721 cells were maintained by the 960...To investigate the effect and mechanism of herbal medicine 960 recipe(960) on reversing the malignant phenotypes of human hepatocellular car-cinoma(HCC) SMMC7721 cells.Methods SMMC7721 cells were maintained by the 960-containing serum derived from rats who had been administered orally with 960 recipe. Cell vitality was detected by MTT assay. The abilities of albumin and alpha fetal protein (AFP) secretion were determined by enzyme linked immunosorbent assay (ELISA). cAMP and cGMP were detected with 125I-cAMP and 125 I-cGMP radioimmune methods, and telomerase activities were assessed using the telomerase PCR ELISA.Results After treated with 960 containing serum, the cell vitality reduced by 30. 7% for 12h, 40.3% for 24h, 56.9% for 48h and 67.7% for 72h; the abilities of albumin secretion enhanced especially on day 6, it increased by 24.5% compared with that of control group; and AFP reduced 62. 5%, significantly lower than that of control; the intracellular cAMP level was elevated, and telomerase展开更多
To investigate the mechanism of 960 recipe regulating the proliferation and apoptosis of human hepatoma SMMC7721 cells.Methods 960 recipe-containing serum was derived from rats that pre-treated with 960 recipe through...To investigate the mechanism of 960 recipe regulating the proliferation and apoptosis of human hepatoma SMMC7721 cells.Methods 960 recipe-containing serum was derived from rats that pre-treated with 960 recipe through gastrogavage, and was added to cultured human hepatoma SMMC-7721 cells while the normal rat serum was tested as control. Cell proliferation was measured with <sup>3</sup>H-TdR incorporation. Cell morphology was tested by acridine orange staining. Cell apoptosis and expressions of p53, bcl-2 and p21<sup>ras</sup> gene protein were analyzed with flow cytometry.Results After treating with 960 recipe, the inhibitory rate of <sup>3</sup>H-TdR was 42.2% for 48h. Cell morphology showed typical apoptotic cells with condensed and fragmented nuclei. There were typical apoptotic peaks in DNA histogram, the apoptotic rate being 21.25% and 27.77% for 24h, 48h respectively. Cell cycle analysis showed that cells were arrested at S-phase by treating with 960 recipe for 24h and at G<sub>0</sub>/G<sub>1</sub> phase for 48h. The expression of p53 increased, but bcl-2 and ras were reduced by treating with 960 recipe for 24h.Conclusion 960 recipe can inhibit proliferation and induce apoptosis of human hepatoma cells, and affect the cell cycle, the expression of oncogene and tumor suppressor gene. These might be the main antihepatoma mechanisms of 960 recipe.展开更多
Objective: To analyze the effectiveness of different acupuncture-moxibustion therapies for neurodynia and skin lesions of acute herpes zoster. Patients and Methods: From April 2007 to October 2009, 500 patients with c...Objective: To analyze the effectiveness of different acupuncture-moxibustion therapies for neurodynia and skin lesions of acute herpes zoster. Patients and Methods: From April 2007 to October 2009, 500 patients with clinical acute herpes zoster were included in the study. They were randomly divided into five groups as follow: electroacupuncture (E);electroacupuncture + cottonpave moxibustion (EC);electroacupuncture + fire acupuncture (EF);electroacupuncture + tapping combined with cupping (ET);control group of western medicine (WM). Results: The time of staunch bleb, scab and scab-off had no obvious statistical difference in the five groups;however, five methods could obviously reduce symptoms of herpes zoster and improved general symptoms. Within five days of treatment, compared to control group, the other four methods could more quickly ameliorate the general symptom of herpes zoster, and EF was superior to EC. In addition to electroacupuncture group, the treatment groups could relieve neuralgia and shortened duration of pain, which was superior to control group treatment. In addition, within first 3 days of treatment, the efficacy of treatment with E, EF or ET was superior to that of EC, and EF was better to E. After five days or end-of-treatment, the efficiency of odynolysis by treatment with EF, ETor EC was no more than E. The incidence of neurodynia was reduced after treatment with EC, EF or ET at 30th, 60th and 90th day, and pain of postherpetic neuralgia was relived. Conclusion: It is a certain advantages for organism reparation, abatement of neurodynia, and reduction of postherpetic neuralgia by acupuncture treatment.展开更多
Objectives： To investigate the safety and efficacy of Yangxinkang Tablets （养心康片） in patients with chronic heart failure （CHF） and syndrome of qi and yin deficiency, blood stasis, and water retention. Methods...Objectives： To investigate the safety and efficacy of Yangxinkang Tablets （养心康片） in patients with chronic heart failure （CHF） and syndrome of qi and yin deficiency, blood stasis, and water retention. Methods： In a double-blinded, randomized, placebo-controlled, multicenter clinical trail, 228 patients with CHF New York Heart Association （NYHA） class Ⅱ or Ⅲ in stage C were assigned by randomized block method to two groups in a 1：1 ratio to undergo either conventional Western treatment or conventional treatment plus Yangxinkang Tablets for 4 weeks. The outcome measure were effect of cardiac function, Chinese medicine （CM） syndromes, scores of symptoms, signs, and quality of life measured by Minnesota Living with heart failure questionnaire （MLHFQ） before and after the treatment. Results： Totally 112 patients were analyzed in the treatment group and 109 in the control group. They were comparable in NYHA functional class, basic parameters and primary diseases before treatment. Cardiac function and CM syndromes were greatly ameliorated in both groups after treatment. Total effective rates of cardiac function and CM syndrome in the treatment group were significantly higher than those in the control group （P〈0.05）. Total symptom score and sign score in the treatment group decreased significantly after treatment （P〈0.01）, which were significantly lower than those in the control group （P〈0.05）. There were statistically significant differences in post-treatment scores of gasp, cough with phlegm, pulmonary rales and jugular vein engorgement between the two groups （P〈0.05 or P〈0.01）. Three MLHFQ scores decreased significantly in both groups after treatment （P〈0.01）. Post-treatment total scale score and physical subscale score in the treatment group and the reduction of them showed statistically significant differences （P〈0.05） as compared with the control group. There was no significant difference between the two groups in emotional subscale score and the reduction after treatment （P〉0.05）. There was no obvious adverse reaction in either group noted during the study. Conclusions： Yangxinkang Tablets were safe and efficacious in improving cardiac function, CM syndromes, symptoms, signs, and quality of life in patients with CHF class Ⅱ or Ⅲ in stage C on the base of conventional treatment.展开更多
Objective： To investigate the effect of pricking blood therapy on behavior and brainstem c-los and c-jun gene expression in migraine rats. Methods： A rat model of migraine was made with nitroglycerol. The rats were ...Objective： To investigate the effect of pricking blood therapy on behavior and brainstem c-los and c-jun gene expression in migraine rats. Methods： A rat model of migraine was made with nitroglycerol. The rats were treated by pricking blood. Rat behavior was observed. Brainstem c-fos and c-jun gene expression was examined by immunohistochemistry. Results： Ear redness improved significantly, the number of head scratchings decreased obviously （P〈0.05） and c-fos and c-jun expression was reduced markedly （P〈0.01） in the treatment group after pricking blood compared with the model group and the blank group. Conclusion： Pricking blood treatment can improve behavior indices and reduce c-fos and c-jun positive expression in migraine rats.展开更多
文摘Having been engaged in teaching, clinical practice and research for many years, Dr. Lai Xinsheng is good at acupuncture and the combined acupuncture and medication therapy to treat refractory diseases with satisfactory therapeutic effect. Sample cases treated by Dr. Lai are selected as follows.
基金Supported by the National Natural Science Foundation of China,No.81900490,No.81670477,and No.81600419
文摘BACKGROUND Accelerated therapeutic treatment should be considered in patients with progressive Crohn’s disease(CD)to prevent complications as well as surgery.Therefore,screening for risk factors and predicting the need for early surgery are of great importance in clinical practice.AIM To establish a model to predict CD-related early surgery.METHODS This was a retrospective study collecting data from CD patients diagnosed at our inflammatory bowel disease center from January 1,2012 to December 31,2016.All data were randomly stratified into a training set and a testing set at a ratio of 8:2.Multivariable logistic regression analysis was conducted with receiver operating characteristic curves constructed and areas under the curve calculated.This model was further validated with calibration and discrimination estimated.A nomogram was finally developed.RESULTS A total of 1002 eligible patients were enrolled with a mean follow-up period of 53.54±13.10 mo.In total,24.25%of patients received intestinal surgery within 1 year after diagnosis due to complications or disease relapse.Disease behavior(B2:OR[odds ratio]=6.693,P<0.001;B3:OR=14.405,P<0.001),smoking(OR=4.135,P<0.001),body mass index(OR=0.873,P<0.001)and C-reactive protein(OR=1.022,P=0.001)at diagnosis,previous perianal(OR=9.483,P<0.001)or intestinal surgery(OR=8.887,P<0.001),maximum bowel wall thickness(OR=1.965,P<0.001),use of biologics(OR=0.264,P<0.001),and exclusive enteral nutrition(OR=0.089,P<0.001)were identified as independent significant factors associated with early intestinal surgery.A prognostic model was established and further validated.The receiver operating characteristic curves and calculated areas under the curves(94.7%)confirmed an ideal predictive ability of this model with a sensitivity of 75.92%and specificity of 95.81%.A nomogram was developed to simplify the use of the predictive model in clinical practice.CONCLUSION This prognostic model can effectively predict 1-year risk of CD-related intestinal surgery,which will assist in screening progressive CD patients and tailoring therapeutic management.
文摘Objective: To study the trauma and stress level of the pelvic fracture treated by 3D printing combined with lateral rectus abdominis approach surgery. Methods: A total of 50 patients with pelvic fractures who were treated in the hospital between July 2015 and Jun 2017 were collected anddivided into control group (n=25) and observation group (n=25) by random number table method. Control group underwent lateral rectus abdominis approach surgery, and the observation group received 3D printingcombined with lateral rectus abdominis approach surgery. The differences in perioperative inflammatory response, coagulation function and stress degree were compared between the two groups. Results: 24 h before surgery, the differences in inflammatory response, coagulation function and stress degree were not statistically significant between the two groups. 24 h after surgery, serum IL-1, TNF-α, CRP, FIB, D-D, Cor, AngⅡ and NE levels of both groups of patients were higher than those 24h before surgery, and serum IL-1, TNF-α, CRP, FIB, D-D, Cor, AngⅡ and NE levels of observation group were lower than those of control group. Conclusion: 3D printing combined with lateral rectus abdominis approach surgery can effectively reduce the degree of surgical trauma and reduce the systemic stress response in patients with pelvic fracture.
文摘To investigate the effect and mechanism of herbal medicine 960 recipe(960) on reversing the malignant phenotypes of human hepatocellular car-cinoma(HCC) SMMC7721 cells.Methods SMMC7721 cells were maintained by the 960-containing serum derived from rats who had been administered orally with 960 recipe. Cell vitality was detected by MTT assay. The abilities of albumin and alpha fetal protein (AFP) secretion were determined by enzyme linked immunosorbent assay (ELISA). cAMP and cGMP were detected with 125I-cAMP and 125 I-cGMP radioimmune methods, and telomerase activities were assessed using the telomerase PCR ELISA.Results After treated with 960 containing serum, the cell vitality reduced by 30. 7% for 12h, 40.3% for 24h, 56.9% for 48h and 67.7% for 72h; the abilities of albumin secretion enhanced especially on day 6, it increased by 24.5% compared with that of control group; and AFP reduced 62. 5%, significantly lower than that of control; the intracellular cAMP level was elevated, and telomerase
基金Supported By the New Star Plan of Guangzhou Committee of Science & Technology (1999-K-006-03) and the Foundation Medical Science of Guangdong Province (No. B 1999149)
文摘To investigate the mechanism of 960 recipe regulating the proliferation and apoptosis of human hepatoma SMMC7721 cells.Methods 960 recipe-containing serum was derived from rats that pre-treated with 960 recipe through gastrogavage, and was added to cultured human hepatoma SMMC-7721 cells while the normal rat serum was tested as control. Cell proliferation was measured with <sup>3</sup>H-TdR incorporation. Cell morphology was tested by acridine orange staining. Cell apoptosis and expressions of p53, bcl-2 and p21<sup>ras</sup> gene protein were analyzed with flow cytometry.Results After treating with 960 recipe, the inhibitory rate of <sup>3</sup>H-TdR was 42.2% for 48h. Cell morphology showed typical apoptotic cells with condensed and fragmented nuclei. There were typical apoptotic peaks in DNA histogram, the apoptotic rate being 21.25% and 27.77% for 24h, 48h respectively. Cell cycle analysis showed that cells were arrested at S-phase by treating with 960 recipe for 24h and at G<sub>0</sub>/G<sub>1</sub> phase for 48h. The expression of p53 increased, but bcl-2 and ras were reduced by treating with 960 recipe for 24h.Conclusion 960 recipe can inhibit proliferation and induce apoptosis of human hepatoma cells, and affect the cell cycle, the expression of oncogene and tumor suppressor gene. These might be the main antihepatoma mechanisms of 960 recipe.
文摘Objective: To analyze the effectiveness of different acupuncture-moxibustion therapies for neurodynia and skin lesions of acute herpes zoster. Patients and Methods: From April 2007 to October 2009, 500 patients with clinical acute herpes zoster were included in the study. They were randomly divided into five groups as follow: electroacupuncture (E);electroacupuncture + cottonpave moxibustion (EC);electroacupuncture + fire acupuncture (EF);electroacupuncture + tapping combined with cupping (ET);control group of western medicine (WM). Results: The time of staunch bleb, scab and scab-off had no obvious statistical difference in the five groups;however, five methods could obviously reduce symptoms of herpes zoster and improved general symptoms. Within five days of treatment, compared to control group, the other four methods could more quickly ameliorate the general symptom of herpes zoster, and EF was superior to EC. In addition to electroacupuncture group, the treatment groups could relieve neuralgia and shortened duration of pain, which was superior to control group treatment. In addition, within first 3 days of treatment, the efficacy of treatment with E, EF or ET was superior to that of EC, and EF was better to E. After five days or end-of-treatment, the efficiency of odynolysis by treatment with EF, ETor EC was no more than E. The incidence of neurodynia was reduced after treatment with EC, EF or ET at 30th, 60th and 90th day, and pain of postherpetic neuralgia was relived. Conclusion: It is a certain advantages for organism reparation, abatement of neurodynia, and reduction of postherpetic neuralgia by acupuncture treatment.
基金Supported by Guangzhou Municipal Science and Technology Bureau Key Technology Research and Development of Pharmaceutical Preparations and Industrial Demonstration projects of China(No.2008A1-E4101)
文摘Objectives： To investigate the safety and efficacy of Yangxinkang Tablets （养心康片） in patients with chronic heart failure （CHF） and syndrome of qi and yin deficiency, blood stasis, and water retention. Methods： In a double-blinded, randomized, placebo-controlled, multicenter clinical trail, 228 patients with CHF New York Heart Association （NYHA） class Ⅱ or Ⅲ in stage C were assigned by randomized block method to two groups in a 1：1 ratio to undergo either conventional Western treatment or conventional treatment plus Yangxinkang Tablets for 4 weeks. The outcome measure were effect of cardiac function, Chinese medicine （CM） syndromes, scores of symptoms, signs, and quality of life measured by Minnesota Living with heart failure questionnaire （MLHFQ） before and after the treatment. Results： Totally 112 patients were analyzed in the treatment group and 109 in the control group. They were comparable in NYHA functional class, basic parameters and primary diseases before treatment. Cardiac function and CM syndromes were greatly ameliorated in both groups after treatment. Total effective rates of cardiac function and CM syndrome in the treatment group were significantly higher than those in the control group （P〈0.05）. Total symptom score and sign score in the treatment group decreased significantly after treatment （P〈0.01）, which were significantly lower than those in the control group （P〈0.05）. There were statistically significant differences in post-treatment scores of gasp, cough with phlegm, pulmonary rales and jugular vein engorgement between the two groups （P〈0.05 or P〈0.01）. Three MLHFQ scores decreased significantly in both groups after treatment （P〈0.01）. Post-treatment total scale score and physical subscale score in the treatment group and the reduction of them showed statistically significant differences （P〈0.05） as compared with the control group. There was no significant difference between the two groups in emotional subscale score and the reduction after treatment （P〉0.05）. There was no obvious adverse reaction in either group noted during the study. Conclusions： Yangxinkang Tablets were safe and efficacious in improving cardiac function, CM syndromes, symptoms, signs, and quality of life in patients with CHF class Ⅱ or Ⅲ in stage C on the base of conventional treatment.
基金Administration of Traditional Chinese Medicine of Guangdong Province (2040049)
文摘Objective： To investigate the effect of pricking blood therapy on behavior and brainstem c-los and c-jun gene expression in migraine rats. Methods： A rat model of migraine was made with nitroglycerol. The rats were treated by pricking blood. Rat behavior was observed. Brainstem c-fos and c-jun gene expression was examined by immunohistochemistry. Results： Ear redness improved significantly, the number of head scratchings decreased obviously （P〈0.05） and c-fos and c-jun expression was reduced markedly （P〈0.01） in the treatment group after pricking blood compared with the model group and the blank group. Conclusion： Pricking blood treatment can improve behavior indices and reduce c-fos and c-jun positive expression in migraine rats.
