Unraveling the distribution of metabolites in traditional Chinese medicine(TCM)provides direct indications for understanding their regulatory and functional basis,which is of paramount significance for better utilizat...Unraveling the distribution of metabolites in traditional Chinese medicine(TCM)provides direct indications for understanding their regulatory and functional basis,which is of paramount significance for better utilization and quality control of medicinal plants[1].Recently,imaging techniques such as near-infrared spectroscopy,Raman spectroscopy,and mass spectrometry(MS)were explored to reveal the spatial context of component accumulation and localization[2,3].展开更多
BACKGROUND A comprehensive literature search shows that Sanqi and Huangjing(SQHJ)can improve diabetes treatment in vivo and in vitro,respectively.However,the combined effects of SQHJ on diabetes mellitus(DM)are still ...BACKGROUND A comprehensive literature search shows that Sanqi and Huangjing(SQHJ)can improve diabetes treatment in vivo and in vitro,respectively.However,the combined effects of SQHJ on diabetes mellitus(DM)are still unclear.AIM To explore the potential mechanism of Panax notoginseng(Sanqi in Chinese)and Polygonati Rhizoma(Huangjing in Chinese)for the treatment of DM using network pharmacology.METHODS The active components of SQHJ and targets were predicted and screened by network pharmacology through oral bioavailability and drug-likeness filtration using the Traditional Chinese Medicine Systems Pharmacology Analysis Platform database.The potential targets for the treatment of DM were identified according to the DisGeNET database.A comparative analysis was performed to investigate the overlapping genes between active component targets and DM treatmentrelated targets.We constructed networks of the active component-target and target pathways of SQHJ using Cytoscape software and then analyzed the gene functions.Using the STRING database to perform an interaction analysis among overlapping genes and a topological analysis,the interactions between potential targets were identified.Gene Ontology(GO)function analyses and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted in DAVID.RESULTS We screened 18 active components from 157 SQHJ components,187 potential targets for active components and 115 overlapping genes for active components and DM.The network pharmacology analysis revealed that quercetin,beta-sitosterol,baicalein,etc.were the major active components.The mechanism underlying the SQHJ intervention effects in DM may involve nine core targets(TP53,AKT1,CASP3,TNF,interleukin-6,PTGS2,MMP9,JUN,and MAPK1).The screening and enrichment analysis revealed that the treatment of DM using SQHJ primarily involved 16 GO enriched terms and 13 related pathways.CONCLUSION SQHJ treatment for DM targets TP53,AKT1,CASP3,and TNF and participates in pathways in leishmaniasis and cancer.展开更多
In this paper,we propose and demonstrate a high-performance mercury ion sensor with sub-nM detection limit,high selectivity,and strong practicability based on the small molecule of the 4-mercaptopyridine(4-MPY)modifie...In this paper,we propose and demonstrate a high-performance mercury ion sensor with sub-nM detection limit,high selectivity,and strong practicability based on the small molecule of the 4-mercaptopyridine(4-MPY)modified tilted fiber Bragg grating surface plasmon resonance(TFBG-SPR)sensing platform.The TFBG-SPR sensor has a rich mode field distribution and a narrow bandw idth,which can detect the microscopic physical and chemical reactions on the sensor surface with high sensitivity without being disturbed by the external temperature.For the environmental compatibility and highly efficient capture of the toxic mercury ion,4-MPY is modified on the sensor surface forming a stable(4-MPY)-Hg-(4-MPY)structure due to the specific combination between the nitrogen of the pyridine moiety and the Hg+via multidentate N-bonding.Moreover,gold nanoparticles(AuNPs)are connected to the sensor surface through the(4-MPY)-Hg-(4-MPY)structure,which could play an important role for signal amplification.Under the optimized conditions,the limit of detection of the sensor for mercury ions detection in the solution is as low as 1.643×10^(-10)M(0.1643 nM),and the detection range is 1×10^(-9)M-1×10^(-5)M.At the same time,the mercury ion spiked detection with tap water shows that the sensor has the good selectivity and reliability in actual water samples.We develop a valuable sensing technology for on-time environmental Hg t detection and in-vivo point of care testing in clinic applications.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.:81773874).
文摘Unraveling the distribution of metabolites in traditional Chinese medicine(TCM)provides direct indications for understanding their regulatory and functional basis,which is of paramount significance for better utilization and quality control of medicinal plants[1].Recently,imaging techniques such as near-infrared spectroscopy,Raman spectroscopy,and mass spectrometry(MS)were explored to reveal the spatial context of component accumulation and localization[2,3].
基金Supported by the Central Government guides local S&T Program of Hebei Province,No.216Z2501GScientific Research Project of Hebei Provincial Market Supervision Administration,No.2021YJ11.
文摘BACKGROUND A comprehensive literature search shows that Sanqi and Huangjing(SQHJ)can improve diabetes treatment in vivo and in vitro,respectively.However,the combined effects of SQHJ on diabetes mellitus(DM)are still unclear.AIM To explore the potential mechanism of Panax notoginseng(Sanqi in Chinese)and Polygonati Rhizoma(Huangjing in Chinese)for the treatment of DM using network pharmacology.METHODS The active components of SQHJ and targets were predicted and screened by network pharmacology through oral bioavailability and drug-likeness filtration using the Traditional Chinese Medicine Systems Pharmacology Analysis Platform database.The potential targets for the treatment of DM were identified according to the DisGeNET database.A comparative analysis was performed to investigate the overlapping genes between active component targets and DM treatmentrelated targets.We constructed networks of the active component-target and target pathways of SQHJ using Cytoscape software and then analyzed the gene functions.Using the STRING database to perform an interaction analysis among overlapping genes and a topological analysis,the interactions between potential targets were identified.Gene Ontology(GO)function analyses and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted in DAVID.RESULTS We screened 18 active components from 157 SQHJ components,187 potential targets for active components and 115 overlapping genes for active components and DM.The network pharmacology analysis revealed that quercetin,beta-sitosterol,baicalein,etc.were the major active components.The mechanism underlying the SQHJ intervention effects in DM may involve nine core targets(TP53,AKT1,CASP3,TNF,interleukin-6,PTGS2,MMP9,JUN,and MAPK1).The screening and enrichment analysis revealed that the treatment of DM using SQHJ primarily involved 16 GO enriched terms and 13 related pathways.CONCLUSION SQHJ treatment for DM targets TP53,AKT1,CASP3,and TNF and participates in pathways in leishmaniasis and cancer.
基金This work was supported by the National Nature Science Foundation of China(Grant Nos.61520106013 and 61727816)Exchange Fund from Key Laboratory of Optical Fiber Sensing and Communications(Ministry of Education of China)(Grant No.ZYGX2019K006)+1 种基金the Fundamental Research Funds for Central Universities(Grant No.DUT19LAB32)the Local Science and Technology Development Fund Projects guided by the central government(Grant No.206Z4801G).
文摘In this paper,we propose and demonstrate a high-performance mercury ion sensor with sub-nM detection limit,high selectivity,and strong practicability based on the small molecule of the 4-mercaptopyridine(4-MPY)modified tilted fiber Bragg grating surface plasmon resonance(TFBG-SPR)sensing platform.The TFBG-SPR sensor has a rich mode field distribution and a narrow bandw idth,which can detect the microscopic physical and chemical reactions on the sensor surface with high sensitivity without being disturbed by the external temperature.For the environmental compatibility and highly efficient capture of the toxic mercury ion,4-MPY is modified on the sensor surface forming a stable(4-MPY)-Hg-(4-MPY)structure due to the specific combination between the nitrogen of the pyridine moiety and the Hg+via multidentate N-bonding.Moreover,gold nanoparticles(AuNPs)are connected to the sensor surface through the(4-MPY)-Hg-(4-MPY)structure,which could play an important role for signal amplification.Under the optimized conditions,the limit of detection of the sensor for mercury ions detection in the solution is as low as 1.643×10^(-10)M(0.1643 nM),and the detection range is 1×10^(-9)M-1×10^(-5)M.At the same time,the mercury ion spiked detection with tap water shows that the sensor has the good selectivity and reliability in actual water samples.We develop a valuable sensing technology for on-time environmental Hg t detection and in-vivo point of care testing in clinic applications.
