Genotoxicity of dichlorvos and butachlor on erythrocytes of Rana zhenhaiensis tadpoles was investigated by the alkaline single-cell gel electrophoresis assay or comet assay.Tadpoles were treated for 24h in the laborat...Genotoxicity of dichlorvos and butachlor on erythrocytes of Rana zhenhaiensis tadpoles was investigated by the alkaline single-cell gel electrophoresis assay or comet assay.Tadpoles were treated for 24h in the laboratory with different concentrations of the testing agents,2.256,4.512,6.768,9.024,11.280mg/L for dichlorvos and 0.292,0.584,0.876,1.168,1.460mg/L for butachlor,to use the comet Assay to test for the significance of dosage responsiveness to an increase in DNA damage,asmeasured by themean DNA tail length-to-width ratio.The concentrations of 4.512mg/L dichlorvos and 0.876mg/L butachlor resulted in highly significant increases in DNA damage of the tadpoles.There were linear correlations between themean DNA tail length-to-width ratio and the concentrations of the two test substances.Our results showed that the two commonly used agricultural chemicals caused dose dependent DNA damage of amphibians,and that comet assaymight be a useful tool formeasuring DNA damage of tadpoles exposed in the field.展开更多
基金granted by the Natural Science Foundation of Fujian,China(2009J01129)the Education Department Foundation of Fujian,China(2007F5033)
文摘Genotoxicity of dichlorvos and butachlor on erythrocytes of Rana zhenhaiensis tadpoles was investigated by the alkaline single-cell gel electrophoresis assay or comet assay.Tadpoles were treated for 24h in the laboratory with different concentrations of the testing agents,2.256,4.512,6.768,9.024,11.280mg/L for dichlorvos and 0.292,0.584,0.876,1.168,1.460mg/L for butachlor,to use the comet Assay to test for the significance of dosage responsiveness to an increase in DNA damage,asmeasured by themean DNA tail length-to-width ratio.The concentrations of 4.512mg/L dichlorvos and 0.876mg/L butachlor resulted in highly significant increases in DNA damage of the tadpoles.There were linear correlations between themean DNA tail length-to-width ratio and the concentrations of the two test substances.Our results showed that the two commonly used agricultural chemicals caused dose dependent DNA damage of amphibians,and that comet assaymight be a useful tool formeasuring DNA damage of tadpoles exposed in the field.
