Objective:To investigate the effect of sevoflurane on tissue permeability of lung ischemiareperfusion injury(LIRI)in rats.Methods:A total of 45 wistar rats were randomly divided into3 groupsⅠ,Ⅱ,Ⅲ.Modified Eppinger ...Objective:To investigate the effect of sevoflurane on tissue permeability of lung ischemiareperfusion injury(LIRI)in rats.Methods:A total of 45 wistar rats were randomly divided into3 groupsⅠ,Ⅱ,Ⅲ.Modified Eppinger method was adopted to establish the rat lung ischemiareperfusion injury model.GroupⅠserved as the control group,groupⅡas ischemia reperfusion group,groupⅢas sevoflurane ischemia-reperfusion group.Blood gas index,lung permeability index(LPI)change,lung tissue pathology change and lung water content were observed and compared between groups of rats at different time points.Results:During ischemia reperfusion,all rats kept balance of the MAP during different time points,SPO_2 of groupⅡandⅢdecreased significantly thanⅠgroup(P<0.05);after reperfusion lung permeability index in GroupⅡandⅢwas higher than the control group significantly(P<0.05),120 min after reperfusion LPI change and iujury of groupⅢwas significantly lower thanⅡgroup(P<0.05);interstitial and alveolar cavity effusion in of groupⅢwere lower than that of groupⅡ.Conclusions:Sevoflurane pretreatment can reduce the lung tissue permeability,and LIRI plays a protective role in LIRI.展开更多
Objective:To study the effect of arsenic trioxide(As_2O_3)combined with ginsenosides Rg3 on inhibiting the NCI-H1299 lung cancer cells and subsistence in nude mice bearing hepatoma.Methods:MTT method was used to measu...Objective:To study the effect of arsenic trioxide(As_2O_3)combined with ginsenosides Rg3 on inhibiting the NCI-H1299 lung cancer cells and subsistence in nude mice bearing hepatoma.Methods:MTT method was used to measure the inhibition effect of(As_2O_3)combined Rg3 on NC1-H1299 cells,and the proliferation inhibiting effect was observed via establishing the transplanted tumor model in vitro.A total of 40 tumor-bearing nude mice were randomly divided into normal saline group,(As_2O_3),Rg3 and As_2O_3+Rg3 group.Transplantation tumor model of lung cancer in nude mice was constructed,followed by injection of certain concentrations of normal saline,As_2O_3,ginseng saponin Rg3 and As_2O_3+Rg3 every day.The survival duration and the tumors size of the mice were recorded and the Kaplan-Meier curve was made;microscopic observation of apoptosis of tumor cells in vivo was done using TUNEL staining.Results:After 72 h of injection.inhibition rate of tumor cell in normal saline group,As_2O_3 group.Rg3 group and As_2O_3+Rg3 group was(5.66±0.31)%,(65.58±4.75)%,(44.69±3.32)%and(82.67±5.43)%,respectively.Inhibition rate of tumor cell in As_2O_3 group.Rg3 groap and As_2O_3+Rg3 group was significantly higher than that of normal saline group(P<0.01);inhibition rate of tumor cells of As_2O_3+Rg3 group was significantly higher than that of the two groups given As_2O_3 or Rg3 alone(P<0.01).The tumor volume of As_2O_3 group,Rg3 group and As_2O_3+Rg3 group shrank to(65.38±3.25)%,(77.68±3.43)%and(42.65±3.55)%of the original,tumor volume of saline group was 1.21 times of the original size(P<0.01);Median survival of saline group,Rg3 group,As_2O_3 group were significantly shorter than that of As_2O_3+Rg3 group(P<0.01);co-ordinated intervention ability of As_2O_3+Rg3 on NCI-H1299 cell was significantly higher than that of As_2O_3 or Rg3,separately.Conclusions:As_2O_3 combined with Rg3 can significantly inhibit prolifaration of NCI-H1299 cells in lung cancer,prolong survival fo tumor-bearing nude mice,and promote tumor cell apoptosis,and have significant effect on lung cancer treatment.展开更多
基金supported by New Medical Technology Import Project in Henan Province(No 2011020113)
文摘Objective:To investigate the effect of sevoflurane on tissue permeability of lung ischemiareperfusion injury(LIRI)in rats.Methods:A total of 45 wistar rats were randomly divided into3 groupsⅠ,Ⅱ,Ⅲ.Modified Eppinger method was adopted to establish the rat lung ischemiareperfusion injury model.GroupⅠserved as the control group,groupⅡas ischemia reperfusion group,groupⅢas sevoflurane ischemia-reperfusion group.Blood gas index,lung permeability index(LPI)change,lung tissue pathology change and lung water content were observed and compared between groups of rats at different time points.Results:During ischemia reperfusion,all rats kept balance of the MAP during different time points,SPO_2 of groupⅡandⅢdecreased significantly thanⅠgroup(P<0.05);after reperfusion lung permeability index in GroupⅡandⅢwas higher than the control group significantly(P<0.05),120 min after reperfusion LPI change and iujury of groupⅢwas significantly lower thanⅡgroup(P<0.05);interstitial and alveolar cavity effusion in of groupⅢwere lower than that of groupⅡ.Conclusions:Sevoflurane pretreatment can reduce the lung tissue permeability,and LIRI plays a protective role in LIRI.
基金supported by New Medical Technology Import Project in Henan Province,Number:2011020113
文摘Objective:To study the effect of arsenic trioxide(As_2O_3)combined with ginsenosides Rg3 on inhibiting the NCI-H1299 lung cancer cells and subsistence in nude mice bearing hepatoma.Methods:MTT method was used to measure the inhibition effect of(As_2O_3)combined Rg3 on NC1-H1299 cells,and the proliferation inhibiting effect was observed via establishing the transplanted tumor model in vitro.A total of 40 tumor-bearing nude mice were randomly divided into normal saline group,(As_2O_3),Rg3 and As_2O_3+Rg3 group.Transplantation tumor model of lung cancer in nude mice was constructed,followed by injection of certain concentrations of normal saline,As_2O_3,ginseng saponin Rg3 and As_2O_3+Rg3 every day.The survival duration and the tumors size of the mice were recorded and the Kaplan-Meier curve was made;microscopic observation of apoptosis of tumor cells in vivo was done using TUNEL staining.Results:After 72 h of injection.inhibition rate of tumor cell in normal saline group,As_2O_3 group.Rg3 group and As_2O_3+Rg3 group was(5.66±0.31)%,(65.58±4.75)%,(44.69±3.32)%and(82.67±5.43)%,respectively.Inhibition rate of tumor cell in As_2O_3 group.Rg3 groap and As_2O_3+Rg3 group was significantly higher than that of normal saline group(P<0.01);inhibition rate of tumor cells of As_2O_3+Rg3 group was significantly higher than that of the two groups given As_2O_3 or Rg3 alone(P<0.01).The tumor volume of As_2O_3 group,Rg3 group and As_2O_3+Rg3 group shrank to(65.38±3.25)%,(77.68±3.43)%and(42.65±3.55)%of the original,tumor volume of saline group was 1.21 times of the original size(P<0.01);Median survival of saline group,Rg3 group,As_2O_3 group were significantly shorter than that of As_2O_3+Rg3 group(P<0.01);co-ordinated intervention ability of As_2O_3+Rg3 on NCI-H1299 cell was significantly higher than that of As_2O_3 or Rg3,separately.Conclusions:As_2O_3 combined with Rg3 can significantly inhibit prolifaration of NCI-H1299 cells in lung cancer,prolong survival fo tumor-bearing nude mice,and promote tumor cell apoptosis,and have significant effect on lung cancer treatment.
