Red wine has a good potential for alleviating atherosclerosis,but the mechanisms related to hepatointestinal circulation remain to be elucidated.This study showed that administration of a high-polyphenol red wine(16 m...Red wine has a good potential for alleviating atherosclerosis,but the mechanisms related to hepatointestinal circulation remain to be elucidated.This study showed that administration of a high-polyphenol red wine(16 mL/(kg·day))for 16 weeks significantly reduced the atherosclerotic lesion in high-fat diet-fed ApoE^(-/-)mice.The total cholesterol(TC)and low-density lipoprotein cholesterol(LDL-C)levels of plasma were lowered by 11.54%and 18.98%.The pro-inflammatory cytokines including interleukin-6(IL-6)and tumor necrosis factorα(TNF-α)levels were decreased by 27.59%and 31.92%.Red wine also reduced triglyceride(TG)level and lipid deposition in the liver,and increased the concentration of total bile acids(TBA).Untargeted metabolomics analysis indicated that red wine modulated the disorder of liver metabolism by regulating sphingolipid signaling pathway,sphingolipid metabolism,glycerophosphlipid metabolism,choline metabolism and bile secretion.16S rRNA sequencing revealed that red wine increased the abundance of Akkermansia and Bifidobacterium and reduced the abundance of Mucispirillum,Romboutsia,Lactobacillus,Bilophila and Blautia,along with the increased concentrations of short-chain fatty acids(SCFAs)in feces.These findings indicated that red wine could exert anti-atherosclerotic effect by regulating gut microbiota,restoring SCFAs,alleviating liver metabolic disorders.展开更多
Edible plant derived exosome-like nanoparticles(ELNs)have been shown to have multiple nutraceutical functions.However,the diversity of plant materials makes the plant derived ELN study challenging.More efforts are sti...Edible plant derived exosome-like nanoparticles(ELNs)have been shown to have multiple nutraceutical functions.However,the diversity of plant materials makes the plant derived ELN study challenging.More efforts are still needed to explore the feasible isolation methods of edible plant derived ELNs and the possible roles of food-derived ELNs in improving human health.In this study,a size exclusion chromatography based method was compared with the traditional ultracentrifugation method to isolate blueberry derived ELNs(B-ELNs),and the miRNA profile of B-ELNs was analyzed by high-throughput sequencing.A total of 36 miRNAs were found to be enriched in B-ELNs compared with berry tissue,and their potential cross-kingdom human gene targets were further predicted.Results showed that size exclusion chromatography was effective for B-ELN isolation.The most abundant miRNAs in B-ELNs mainly belonged to the miR166 family and miR396 family.Target gene prediction indicated that B-ELNs could potentially regulate pathways related to the human digestive system,immune system and infectious diseases.展开更多
High-pressure carbon dioxide(HPCD)could effectively suppress the yellowing phenomenon in Chinese water chestnut(CWC)by significantly inhibiting the activity of the key enzyme-phenylalanine ammonia-lyase(PAL).However,t...High-pressure carbon dioxide(HPCD)could effectively suppress the yellowing phenomenon in Chinese water chestnut(CWC)by significantly inhibiting the activity of the key enzyme-phenylalanine ammonia-lyase(PAL).However,the molecular mechanism underlying this inhibition remains unclear.In this study,we investigated the structural changes and inhibition mechanisms of PAL from CWC subjected to HPCD treatment.Homology modeling was used to construct the 3D structure of CWC PAL.The results showed that CWC PAL was highly conserved and had higherα-helix content.Molecular dynamics simulations and molecular docking were utilized to investigate the non-covalent interaction between CO_(2) and PAL.Our findings revealed that CO_(2) induced conformational changes in PAL via hydrogen bonds,polar interactions,and hydrophobic interactions,thereby increasing PAL’sα-helix structure and preventing the substrate L-Phe from entering its active site.Additionally,HPCD treatment caused the movement of the Phe-111 residue in the inner lid-loop,resulting in steric hindrance and destruction of the active center of PAL.These results provide a theoretical basis for expanding the appli-cation of HPCD in the food processing industry.展开更多
The self-assembled peptide-based hydrogels have been widely utilized in foods.However,the self-assembled hydrogels still have some shortages such as low mechanical properties and hydrogel stabilities.Herein,we designe...The self-assembled peptide-based hydrogels have been widely utilized in foods.However,the self-assembled hydrogels still have some shortages such as low mechanical properties and hydrogel stabilities.Herein,we designed two co-assembled C_(13)-WS hydrogels conjugated by Gallic Acid(CA)and Epigallocatechin Gallate(EGCG).The introduction of CA and EGCG into C_(13)-WS hydrogels can significantly improve the mechanical properties and hydrogel stabilities.The storage modulus(G’)values of co-assembled C_(13)-WS/CA and C_(13)-WS/EGCG were 13.33 KPa and 16 KPa,respectively.From transmission electron microscope(TEM)results,CA and EGCG contributed to the rearrangement of C_(13)-WS molecules during co-assembly.Moreover,the self-/co-assembled C_(13)-WS hydrogels showed antimicrobial activity against E.coli,P.aeruginosa,and S.epidermidis.The introduction of CA and EGCG significantly enhanced the antibacterial activity of C_(13)-WS hydrogels.Our findings provide a novel strategy for the development of stable supramolecular antibacterial short peptide hydrogels through co-assembly.展开更多
基金the research funds from the National Natural Science Foundation of China(32272294)。
文摘Red wine has a good potential for alleviating atherosclerosis,but the mechanisms related to hepatointestinal circulation remain to be elucidated.This study showed that administration of a high-polyphenol red wine(16 mL/(kg·day))for 16 weeks significantly reduced the atherosclerotic lesion in high-fat diet-fed ApoE^(-/-)mice.The total cholesterol(TC)and low-density lipoprotein cholesterol(LDL-C)levels of plasma were lowered by 11.54%and 18.98%.The pro-inflammatory cytokines including interleukin-6(IL-6)and tumor necrosis factorα(TNF-α)levels were decreased by 27.59%and 31.92%.Red wine also reduced triglyceride(TG)level and lipid deposition in the liver,and increased the concentration of total bile acids(TBA).Untargeted metabolomics analysis indicated that red wine modulated the disorder of liver metabolism by regulating sphingolipid signaling pathway,sphingolipid metabolism,glycerophosphlipid metabolism,choline metabolism and bile secretion.16S rRNA sequencing revealed that red wine increased the abundance of Akkermansia and Bifidobacterium and reduced the abundance of Mucispirillum,Romboutsia,Lactobacillus,Bilophila and Blautia,along with the increased concentrations of short-chain fatty acids(SCFAs)in feces.These findings indicated that red wine could exert anti-atherosclerotic effect by regulating gut microbiota,restoring SCFAs,alleviating liver metabolic disorders.
基金supported by the National Natural Science Foundation of China(31701561)。
文摘Edible plant derived exosome-like nanoparticles(ELNs)have been shown to have multiple nutraceutical functions.However,the diversity of plant materials makes the plant derived ELN study challenging.More efforts are still needed to explore the feasible isolation methods of edible plant derived ELNs and the possible roles of food-derived ELNs in improving human health.In this study,a size exclusion chromatography based method was compared with the traditional ultracentrifugation method to isolate blueberry derived ELNs(B-ELNs),and the miRNA profile of B-ELNs was analyzed by high-throughput sequencing.A total of 36 miRNAs were found to be enriched in B-ELNs compared with berry tissue,and their potential cross-kingdom human gene targets were further predicted.Results showed that size exclusion chromatography was effective for B-ELN isolation.The most abundant miRNAs in B-ELNs mainly belonged to the miR166 family and miR396 family.Target gene prediction indicated that B-ELNs could potentially regulate pathways related to the human digestive system,immune system and infectious diseases.
基金supported by the Fundamental Research Funds for the Central Universities(2662020SPPY012).
文摘High-pressure carbon dioxide(HPCD)could effectively suppress the yellowing phenomenon in Chinese water chestnut(CWC)by significantly inhibiting the activity of the key enzyme-phenylalanine ammonia-lyase(PAL).However,the molecular mechanism underlying this inhibition remains unclear.In this study,we investigated the structural changes and inhibition mechanisms of PAL from CWC subjected to HPCD treatment.Homology modeling was used to construct the 3D structure of CWC PAL.The results showed that CWC PAL was highly conserved and had higherα-helix content.Molecular dynamics simulations and molecular docking were utilized to investigate the non-covalent interaction between CO_(2) and PAL.Our findings revealed that CO_(2) induced conformational changes in PAL via hydrogen bonds,polar interactions,and hydrophobic interactions,thereby increasing PAL’sα-helix structure and preventing the substrate L-Phe from entering its active site.Additionally,HPCD treatment caused the movement of the Phe-111 residue in the inner lid-loop,resulting in steric hindrance and destruction of the active center of PAL.These results provide a theoretical basis for expanding the appli-cation of HPCD in the food processing industry.
基金supported by China Agriculture Research System of MOF and MARA(CARS-26-07B)Fundamental Research Funds for the Central Universities(2662019PY016,2662020SPPY011)Hubei Province Technology Innovation Special Major Project(2018ABA072).
文摘The self-assembled peptide-based hydrogels have been widely utilized in foods.However,the self-assembled hydrogels still have some shortages such as low mechanical properties and hydrogel stabilities.Herein,we designed two co-assembled C_(13)-WS hydrogels conjugated by Gallic Acid(CA)and Epigallocatechin Gallate(EGCG).The introduction of CA and EGCG into C_(13)-WS hydrogels can significantly improve the mechanical properties and hydrogel stabilities.The storage modulus(G’)values of co-assembled C_(13)-WS/CA and C_(13)-WS/EGCG were 13.33 KPa and 16 KPa,respectively.From transmission electron microscope(TEM)results,CA and EGCG contributed to the rearrangement of C_(13)-WS molecules during co-assembly.Moreover,the self-/co-assembled C_(13)-WS hydrogels showed antimicrobial activity against E.coli,P.aeruginosa,and S.epidermidis.The introduction of CA and EGCG significantly enhanced the antibacterial activity of C_(13)-WS hydrogels.Our findings provide a novel strategy for the development of stable supramolecular antibacterial short peptide hydrogels through co-assembly.
