The experimental model of traumatic brain injury was established in Sprague-Dawley rats according to Feeney＇s free falling method. The brains were harvested at 2, 6 and 24 hours, and at 3 and 5 days after injury. Cha...The experimental model of traumatic brain injury was established in Sprague-Dawley rats according to Feeney＇s free falling method. The brains were harvested at 2, 6 and 24 hours, and at 3 and 5 days after injury. Changes in brain water content were determined using the wet and dry weights. Our results showed that water content of tissue significantly increased after traumatic brain injury, and reached minimum at 24 hours. Hematoxylin-eosin staining revealed pathological impairment of brain tissue at each time point after injury, particularly at 3 days, with nerve cell edema, degenera- tion, and necrosis observed, and the apoptotic rate significantly increased. Immunohistochemistry and western blot analysis revealed that the expression of occludin at the injured site gradually de- creased as injury time advanced and reached a minimum at 3 days after injury; the expression of connexin 43 gradually increased as injury time advanced and reached a peak at 24 hours after in-jury. The experimental findings indicate that changes in occludin and connexin 43 expression were consistent with the development of brain edema, and may reflect the pathogenesis of brain injury.展开更多
Objectives:This study aimed to investigate the symmetry of the Chinese pelvis.Methods:Computed tomography scan images of each of 50 Chinese pelvises were converted to 3D models and the left sides of the pelvises were ...Objectives:This study aimed to investigate the symmetry of the Chinese pelvis.Methods:Computed tomography scan images of each of 50 Chinese pelvises were converted to 3D models and the left sides of the pelvises were reflected on Mimics software.Then,the reflected left side model was aligned with the right side using the closest point algorithm function of Geomagic software to perform symmetry analysis.The volume and surface area of either side of the pelvises were also calculated.The mean standard deviation(SD),the mean percentage of permissible deviations within the±2mm range,the percentage differences in volume and surface area were measured to compare pelvic symmetry.In addition,the distribution of pelvic bilateral symmetry associated with both age and sex were compared.Results:The mean SD was 1.15±0.16mm and the mean percentage of permissible deviations was 90.82%±4.67%.The deviation color maps showed that the specific areas of asymmetry were primarily localized to major muscle or ligament attachment sites and the sacroiliac joint surfaces.There was no significant difference between the bilateral sides of the pelvis in either volume or surface area.Additionally,no difference in any indexes was exhibited in relation to sex and age distribution.展开更多
基金supported by the Natural Science Foundation of Guangdong Province,No.10151600101000002
文摘The experimental model of traumatic brain injury was established in Sprague-Dawley rats according to Feeney＇s free falling method. The brains were harvested at 2, 6 and 24 hours, and at 3 and 5 days after injury. Changes in brain water content were determined using the wet and dry weights. Our results showed that water content of tissue significantly increased after traumatic brain injury, and reached minimum at 24 hours. Hematoxylin-eosin staining revealed pathological impairment of brain tissue at each time point after injury, particularly at 3 days, with nerve cell edema, degenera- tion, and necrosis observed, and the apoptotic rate significantly increased. Immunohistochemistry and western blot analysis revealed that the expression of occludin at the injured site gradually de- creased as injury time advanced and reached a minimum at 3 days after injury; the expression of connexin 43 gradually increased as injury time advanced and reached a peak at 24 hours after in-jury. The experimental findings indicate that changes in occludin and connexin 43 expression were consistent with the development of brain edema, and may reflect the pathogenesis of brain injury.
基金Funding information Clinical Research Startup Program of Southern Medical University by High‐level University Construction Funding of Guangdong Provincial Department of Education,Grant/Award Number:LC2016ZD032National Natural Science Foundation of China,Grant/Award Numbers:81772428,82072411+1 种基金Special Program of Guangdong Frontier and Key Technological Innovation,Grant/Award Number:2015B010125006Innovation fund cultivation project of National Clinical ResearchCenter for Orthopedics Sports Medicine&Rehabilitation,Grant/Award Number:2021‐NCRC‐CXJJPY‐06。
文摘Objectives:This study aimed to investigate the symmetry of the Chinese pelvis.Methods:Computed tomography scan images of each of 50 Chinese pelvises were converted to 3D models and the left sides of the pelvises were reflected on Mimics software.Then,the reflected left side model was aligned with the right side using the closest point algorithm function of Geomagic software to perform symmetry analysis.The volume and surface area of either side of the pelvises were also calculated.The mean standard deviation(SD),the mean percentage of permissible deviations within the±2mm range,the percentage differences in volume and surface area were measured to compare pelvic symmetry.In addition,the distribution of pelvic bilateral symmetry associated with both age and sex were compared.Results:The mean SD was 1.15±0.16mm and the mean percentage of permissible deviations was 90.82%±4.67%.The deviation color maps showed that the specific areas of asymmetry were primarily localized to major muscle or ligament attachment sites and the sacroiliac joint surfaces.There was no significant difference between the bilateral sides of the pelvis in either volume or surface area.Additionally,no difference in any indexes was exhibited in relation to sex and age distribution.
