Hydrogel electrolytes hold great potential in flexible zinc ion supercapacitors(ZICs)due to their high conductivity,good safety,and flexibility.However,freezing of electrolytes at low temperature(subzero)leads to dras...Hydrogel electrolytes hold great potential in flexible zinc ion supercapacitors(ZICs)due to their high conductivity,good safety,and flexibility.However,freezing of electrolytes at low temperature(subzero)leads to drastic reduction in ionic conductivity and mechanical properties that deteriorates the performance of flexible ZICs.Besides,the mechanical fracture during arbitrary deformations significantly prunes out the lifespan of the flexible device.Herein,a Zn^(2+)and Li^(+)co-doped,polypyrrole-dopamine decorated Sb_(2)S_(3)incorporated,and polyvinyl alcohol/poly(N-(2-hydroxyethyl)acrylamide)double-network hydrogel electrolyte is constructed with favorable mechanical reliability,anti-freezing,and self-healing ability.In addition,it delivers ultra-high ionic conductivity of 8.6 and 3.7 S m^(-1)at 20 and−30°C,respectively,and displays excellent mechanical properties to withstand tensile stress of 1.85 MPa with tensile elongation of 760%,together with fracture energy of 5.14 MJ m^(-3).Notably,the fractured hydrogel electrolyte can recover itself after only 90 s of infrared illumination,while regaining 83%of its tensile strain and almost 100%of its ionic conductivity during−30–60°C.Moreover,ZICs coupled with this hydrogel electrolyte not only show a wide voltage window(up to 2 V),but also provide high energy density of 230 Wh kg^(-1)at power density of 500 W kg^(-1)with a capacity retention of 86.7%after 20,000 cycles under 20°C.Furthermore,the ZICs are able to retain excellent capacity even under various mechanical deformation at−30°C.This contribution will open up new insights into design of advanced wearable flexible electronics with environmental adaptability and long-life span.展开更多
Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the ...Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased body.To address this, primary cells that can be stably passaged were isolated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this study.Next, an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells, and the obtained nucleic acid aptamers were affinity verified at the cellular level.Finally, to answer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers, we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model, and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.展开更多
Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduc...Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduce the transmission of gastroenteritis.In the study,a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres,and a closed-cassette-based,integrated,reagent-ambient storage,on-site instant detection platform for norovirus GII was constructed using a commercial,fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection,with a sensitivity of 10 copies/μL,with no cross-reactivity with other 5 viruses.For 28 simulated samples,the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction(RT-qPCR)assays after conventional laboratory nucleic acid extraction.The entire process can be finished in about 1 h,which is ideal for immediate rapid detection.展开更多
The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in late 2019 has negatively affected people's lives and productivity.Because the mode of transmission of SARS-CoV-2 is of great concern,th...The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in late 2019 has negatively affected people's lives and productivity.Because the mode of transmission of SARS-CoV-2 is of great concern,this review discusses the sources of virus aerosols and possible transmission routes.First,we discuss virus aerosol collection methods,including natural sedimentation,solid impact,liquid impact,centrifugal,cyclone and electrostatic adsorption methods.Then,we review common virus aerosol detection methods,including virus culture,metabolic detection,nucleic acid-based detection and immunology-based detection methods.Finally,possible solutions for the detection of SARS-CoV-2 aerosols are introduced.Point-of-care testing has long been a focus of attention.In the near future,the development of an instrument that integrates sampling and output results will enable the real-time,automatic monitoring of patients.展开更多
Novel hydrophilic NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+double-jacket microtubes(DJMTs)with upconversion/downconversion dual-mode luminescence were designed and prepared through epitaxial growth of NaGdF4:Ce^3+,Eu^3+she...Novel hydrophilic NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+double-jacket microtubes(DJMTs)with upconversion/downconversion dual-mode luminescence were designed and prepared through epitaxial growth of NaGdF4:Ce^3+,Eu^3+shell onto the NaYF4:Yb^3+,Tm^3+microtube via poly(acrylic acid)(PAA)mediated hydrothermal method.It is demonstrated that PAA ligand played an important role in guiding the direct growth of NaGdF4:Ce^3+,Eu^3+shell onto the surface of NaYF4:Yb^3+,Tm^3+parent microtubes.The growth of NaGdF4:Ce^3+,Eu^3+shell experienced a crystal phase transition fromβ-NaGdF4 andβ-NaYF4 mixture toβ-NaYF4@NaGdF4 composite crystal,and morphology evolution from mixture ofβ-NaGdF4:Ce^3+,Eu^3+nanorods andβ-NaYF4:Yb^3+,Tm^3+microtubes to NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+DJMTs.The formation mechanism of DJMTs was the dissolution−renucleation ofβ-NaGdF4:Ce^3+,Eu^3+nanorods and the growth ofβ-NaGdF4:Ce^3+,Eu^3+shell via the classical Ostwald ripening mechanism.The as-prepared DJMTs could exhibit blue upconversion and red downconversion luminescence,which was further made into environmentally benign luminescent inks for creating highly secured and fluorescent-based anti-counterfeiting patterns via inkjet printing.展开更多
Palladium-supported cobalt hydroxide(Co(OH)_(2)-Pd) nanoplates were fabricated in an aqueous solution and employed as a catalyst for the reduction of 4-nitrophenol.For the preparation of Co(OH)2-Pd,Pd nanoparticles we...Palladium-supported cobalt hydroxide(Co(OH)_(2)-Pd) nanoplates were fabricated in an aqueous solution and employed as a catalyst for the reduction of 4-nitrophenol.For the preparation of Co(OH)2-Pd,Pd nanoparticles were anchored on the Co(OH)_(2) nanoplates after the reduction of Na;PdCl;by ascorbic acid in the absence of a stabilizer at room temperature.The observations under transmission and scanning electron microscopy reveal that Pd nanoparticles with a size of 2-5 nm are uniformly dispersed on the surface of the Co(OH)_(2) nanoplates.In catalytic test,the conversion of 4-nitrophenol to 4-aminophenol is completed within 6 min in the presence of Co(OH)_(2)-Pd(1000) nanoplates with2.18 at.% Pd,and the corresponding kinetic constant is 0.0089 s;in the first test.The catalyst retains relatively high activity after several cycles.The results demonstrate that the Co(OH)_(2)-Pd(1000) nanoplates exhibit high catalytic activity toward the reduction of 4-nitrophenol in the presence of NaBH;.展开更多
Herein,we fabricated a flexible semidry electrode with excellent mechanical performance,satisfactory self-adhesiveness,and low-contact impedance using physical/chemical crosslinked polyvinyl alcohol/polyacrylamide dua...Herein,we fabricated a flexible semidry electrode with excellent mechanical performance,satisfactory self-adhesiveness,and low-contact impedance using physical/chemical crosslinked polyvinyl alcohol/polyacrylamide dual-network hydrogels(PVA/PAM DNHs)as an efficient saline reservoir.The resultant PVA/PAM DNHs showed admirable adhesive and compliance to the hairy scalp,facilitating the establishment of a robust electrode/skin interface for biopotential signal transmission.Moreover,the PVA/PAM DNHs steadily released trace saline onto the scalp to achieve the minimized potential drift(1.47±0.39 mV/min)and low electrode–scalp impedance(18.2±8.9 kΩ@10 Hz).More importantly,the application feasibility of real-world brain−computer interfaces(BCIs)was preliminarily validated by 10 participants using two classic BCI paradigms.The mean temporal cross-correlation coefficients between the semidry and wet electrodes in the eyes open/closed and the N200 speller paradigms are 0.919±0.054 and 0.912±0.050,respectively.Both electrodes demonstrate anticipated neuroelectrophysiological responses with similar patterns.This semidry electrode could also effectively capture robust P-QRS-T peaks during electrocardiogram recording.Considering their outstanding advantages of fast setup,user-friendliness,and robust signals,the proposed PVA/PAM DNH-based electrode is a promising alternative to wet electrodes in biopotential signal acquisition.展开更多
The feature of the surface coating can affect important properties of iron oxide nanoparticles(IONPs), it is therefore critical for further understanding how these materials react to physiological conditions, which is...The feature of the surface coating can affect important properties of iron oxide nanoparticles(IONPs), it is therefore critical for further understanding how these materials react to physiological conditions, which is still needed to fully exploit the potential of IONPs for their theranostic applications. In this work, we prepared IONPs which surface were modified with citric acid(CA), chitosan(CS) and folic acid conjugated chitosan(FA-g-CS). respectively. Their physicochemical properties were investigated using FT-IR, TEM,powder XRD, VSM, TGA, DLS and zeta potential. We found that CA-IONP dispersion was composed of monocrystalline particles while CS-IONP and FA-g-CS-IONP were composed of polycrystalline aggregates. All IONPs retained the crystalline structure of magnetite and exhibited the superparamagnetic behavior. Their saturation magnetization decreased with the increase in the amount of their organic coatings. Their drug loading capacities, drug release patterns and in vitro anticancer efficiencies were studied by using doxorubicin(DOX) as a model drug. DOX@CS-IONP and DOX@FA-g-CSIONP exhibited lower drug loading while showing higher water dispersity when compared with DOX@CA-IONP. All IONPs were surface charged and they tended to agglomerate in medium with high pH value and ionic strength. In the presence of chitosan or FA-g-CS coatings, their DOX release rate was slowed down compared with that of DOX@CA-IONP. Unloaded IONPs exhibited nearly no cytotoxicity on both cancer cells and normal cells in the presence of chitosan and FA-g-CS when compared with CA-IONP which presented high cytotoxicity. However, DOX@FA-g-CS-IONP showed significantly cytotoxicity on folate receptors(FRs) positive breast cancer cells while exhibiting nearly no cytotoxicity on FRs negative normal cells. Results presented in this study were valuable to the design and fabrication of IONPs-based system for better theranostic applications.展开更多
Point-of-care nucleic acid testing(POCNAT) has played an important role in the outbreak of infectious diseases(e.g., COVID-19) over recent years. POCNAT aims to realize the rapid, simple and automatic detection of nuc...Point-of-care nucleic acid testing(POCNAT) has played an important role in the outbreak of infectious diseases(e.g., COVID-19) over recent years. POCNAT aims to realize the rapid, simple and automatic detection of nucleic acid. Thanks to the development of manufacturing technology, electronic information technology, artificial intelligence technology, and biological information technology in recent years, the development of the POCNAT device has led to significant advancement. Instead of the normal nucleic acid detection methods used in the laboratory, some novel experimental carriers have been applied, such as chips, cartridges and papers. The application of these experimental carriers has realized the automation and integration of nucleic acid detection. The entire process of nucleic acid detection is normally divided into three steps(nucleic acid extraction, target amplification and signal detection). All of the reagents required by the process can be pre-stored on these experimental carriers, without unnecessary manual operation. Furthermore, all of the processes are carried out in this experimental carrier, with the assistance of a specific control device. Although they are complicated to manufacture and precise in design,their application provides a significant step forwards in nucleic acid detection and realizes the integration of nucleic acid detection. This technology has great potential in the field of point-of-care molecular diagnostics in the future. This paper focuses on the relevant content of these experimental carriers.展开更多
Binary transition metal sulfides are hotly investigated in advanced energy storage devices because of their ultra-high reversible capacity.Nevertheless,the unsatisfied rate capability and cycling stability still hinde...Binary transition metal sulfides are hotly investigated in advanced energy storage devices because of their ultra-high reversible capacity.Nevertheless,the unsatisfied rate capability and cycling stability still hinder their practical application.Herein,hierarchical nanoporous carbon@NiCo_(2)S_(4)(HNCMs@NCS)composites with coreshell flower-like structures were prepared by in situ growing of NiCo_(2)S_(4) nanosheets on HNCMs through a hydro thermal-as sis ted template sacrificial method.Benefiting from a synergistic effect between the NiCo_(2)S_(4)shell with high specific capacity and the HNCMs with unique porous structure,the synthesized flower-like HNCMs@NCS composites exhibit extraordinary electrochemical performances,including a high capacity of 346.9 mAh·g^(-1)at 1 A·g^(-1),superb rate property with86.4%initial capacity at 30 A·g^(-1)and predominant cycle stability with 81.2%capacity retention after 5000 cycles.Furthermore,the resulting HNCMs@NCS cathode was coupled with the chemical-activated HNCMs(AHNCMs)anode to construct a hybrid supercapacitor device.The asfabricated device exhibits superior energy density(49.9 Wh·kg^(-1)at 802 W·kg^(-1))and ultra-high power density(24 kW·kg^(-1)at 29.5 Wh·kg^(-1)).This fascinating result further demonstrates the tremendous prospect of the synthesized HNCMs@NCS composites as high-performance supercapacitor electrode materials.展开更多
Compared with other types of breast cancer,triple-negative breast cancer(TNBC)has the characteristics of a high degree of malignancy and poor prognosis.Early diagnosis of TNBC through biological markers and timely dev...Compared with other types of breast cancer,triple-negative breast cancer(TNBC)has the characteristics of a high degree of malignancy and poor prognosis.Early diagnosis of TNBC through biological markers and timely development of effective treatment methods can reduce its mortality.Many Research experiments have confirmed that some specific mi RNA expression profiles in TNBC can used as markers for early diagnosis.However,detecting the expression profiles of multiple groups of miRNAs according to traditional detection methods is complicated and consumes many samples.To address this issue,we developed a method for high-throughput,high-sensitivity quantitative detection of multiple sets of miRNAs(including mi R-16,mi R-21,mi R-92,mi R-199,and mi R-342)specifically expressed in TNBC by rolling circle amplification(RCA)on fluorescence-encoded microspheres.Through the optimization of reaction system conditions,the developed method showed an extensive linear dynamic range and high sensitivity for all five miRNAs with the lowest limit of detection of 2 fmol/L.Meanwhile,this high-throughput detection method also appeared reasonable specificity.Only in the presence of a specific target miRNA,the fluorescence signal on the correspondingly encoded microspheres is significantly increased,while the fluorescence signal on other non-correspondingly encoded microspheres is almost negligible.Furthermore,this process exhibited good recovery and reproducibility in serum.The advantages of this method allow us to more conveniently obtain the expression profiles of multiple groups of TNBC-associated mi RNAs,which is beneficial for the early detection of TNBC.展开更多
Enterocytozoon hepatopenaei(EHP)infection has seriously affected prawn culture globally.The symptoms of the infection are not apparent,and traditional detection methods are time consuming and low in accuracy.We develo...Enterocytozoon hepatopenaei(EHP)infection has seriously affected prawn culture globally.The symptoms of the infection are not apparent,and traditional detection methods are time consuming and low in accuracy.We developed a new onsite rapid testing device(size 18.8×16.7×6.6 cm^(3))for EHP based on magnesium pyrophosphate precipitation and facilitated by loop mediated isothermal amplification(LAMP).The design and fabrication of the device enables efficient light absorbance.The device has a highly sensitive detector,high-precision thermal controller,and humanized touch screen.The temperature control precision of the device is 0.2-0.3℃ at 60℃,63℃,and 65℃.The coefficients of variation values(CVV)of the luminous power in one channel at light on and off were found to be 0.0097 and 0.0014,respectively,within 1 h.The CVV of the background,luminous power,and values of eight PCR tubes filled with pure water were all less than 5%.In the EHP experiment,eight samples(including seven positive and one negative)confirmed the effectiveness of the device,and four positive and four negative samples verified whether cross-contamination exists.Among them,the rise time of the curve was about 15 min.These results assert that the developed device exhibits enhanced stability and uniformity and has excellent performance with high sensitivity,good specificity,and low testing time.Moreover,the optimal and minimum absorbance range was 555-655 nm for monitoring the production of LAMP.展开更多
Phthalates esters(PAEs) are extensively used as additives for polymers in plastic, particularly in polyvinyl chloride(PVC) and polyethylene terephthalate(PET). These compounds are not part of the polymer chains and ca...Phthalates esters(PAEs) are extensively used as additives for polymers in plastic, particularly in polyvinyl chloride(PVC) and polyethylene terephthalate(PET). These compounds are not part of the polymer chains and can be released easily from products and migrate into beverages and foods that come into direct contact, causing environmental and human health impacts. Simple and rapid detection of such substances is of great significance for ensuring environmental food safety and consumer health. At present, optical sensor and electrochemical sensor detection technologies have been applied to PAEs detection due to their advantages, such as simple, rapid, low cost, high sensitivity, simple operation, portability and high specificity. They can make up for the shortcomings of chromatographic detection technology, such as expensive equipment, cumbersome operation, the need for professional and technical personnel, and difficulty in achieving a large number of sample screening objectives. In this paper, research progress on optical sensors and electrochemical sensors for the detection of phthalates in recent ten years is reviewed and discussed. This is helpful to better understand preparation methods for sensors and their detection mechanisms for phthalates. The review will also be used in developing a more effective trace detection sensor for phthalates.展开更多
Up to now, it is always a delicate and challenging task to clean and protect Xuan paper ink painting. Xuan paper is composed of cellulose with a rough surface and easy to absorb dust, which leads to poor cleaning effe...Up to now, it is always a delicate and challenging task to clean and protect Xuan paper ink painting. Xuan paper is composed of cellulose with a rough surface and easy to absorb dust, which leads to poor cleaning effect and irreversible damage in traditional cleaning process. Hydrogel is one of the most effective tools to clean the artworks. However, in the practice of cleaning Xuan paper, most hydrogels cannot achieve fine cleaning result due to the interfacial adhesion issues. Herein, to protectively and effectively clean Xuan paper, using physical yet strong poly(vinyl alcohol)/poly(N-(2-hydroxyethyl) acrylamide)(PVA/PHEAA)hydrogel with suitable stiffness as the model, the interfacial adhesion between hydrogel and Xuan paper was systematically investigated, and various technologies were used to evaluate the cleaning effect. A critical interfacial adhesive energy(< 4 J/m^(2))is found to achieve the protective and effective cleaning purpose. To the best of our knowledge, this is the first report to estimate the adhesion of hydrogel on the cleaning of paper artwork, which will provide a new viewport in the conservation practice.展开更多
The SARS–CoV–2 virus is released from an infectious source(such as a sick person)and adsorbed on aerosols,which can form pathogenic microorganism aerosols,which can affect human health through airborne transmission....The SARS–CoV–2 virus is released from an infectious source(such as a sick person)and adsorbed on aerosols,which can form pathogenic microorganism aerosols,which can affect human health through airborne transmission.Efficient sampling and accurate detection of microorganisms in aerosols are the premise and basis for studying their properties and evaluating their hazard.In this study,we built a set of sub-micron aerosol detection platform,and carried out a simulation experiment on the SARS–CoV–2 aerosol in the air by wet-wall cyclone combined with immunomagnetic nanoparticle adsorption sampling and ddPCR.The feasibility of the system in aerosol detection was verified,and the influencing factors in the detection process were experimentally tested.As a result,the sampling efficiency was 29.77%,and extraction efficiency was 98.57%.The minimum detection limit per unit volume of aerosols was 250 copies(102copies/m L,concentration factor 2.5).展开更多
Extracellular vesicles(EVs)are cell-derived nanosized vesicles widely recognized for their critical roles in various pathophysiological processes.Molecular analysis of EVs is currently being considered an emerging too...Extracellular vesicles(EVs)are cell-derived nanosized vesicles widely recognized for their critical roles in various pathophysiological processes.Molecular analysis of EVs is currently being considered an emerging tool for diseases diagnosis.However,the small size and heterogeneity of EVs has staggered the EVs research for diseases diagnosis.DNA nanotechnology enables self-assembly of versatile DNA nanostructures and has shown enormous potential in assisting EVs biosensing.In this review,we briefly introduce the recent advances in DNA nanotechnology approaches for EVs detection.The approaches were categorized based on the dimension of DNA nanostructures.We provide critical evaluation of these approaches,and summarize the pros and cons of specific methods.Further,we discuss the challenges and future perspectives in this field.展开更多
Canine parvovirus type 2(CPV-2) infection is the most lethal disease of dogs with higher mortality in puppies worldwide.In today’s world,dogs are an integral part of our communities as well as dogs breeding and reari...Canine parvovirus type 2(CPV-2) infection is the most lethal disease of dogs with higher mortality in puppies worldwide.In today’s world,dogs are an integral part of our communities as well as dogs breeding and rearing has become a lucrative business.Therefore,a fast,accurate,portable,and costeffective CPV-2 detection method with the ability for on-site detection is highly desired.In this study,we for the first time proposed a nanosystem for CPV-2 DNA detection with RNA-guided RNA endonuclease Cas13 a,which upon activation results in collateral RNA degradation.We expressed LwCasl3 a in prokaryotic expression system and purified it through nickel column.Activity of Cas13 a was verified by RNA-bound fluorescent group while using a quenched fluorescent probe as signals.Further Cas13 a was combined with Recombinase polymerase amplification(RPA) and T7 transcription to establish molecular detection system termed specific high-sensitivity enzymatic reporter un-locking(SHERLOCK) for sensitive detection of CPV-2 DNA.This nanosystem can detect 100 amol/L CPV-2 DNA within 30 min.The proposed nanosystem exhibited high specificity when tested for CPV-2 and other dog viruses.This CRISPR-Cas13 a mediated sensitive detection approach can be of formidable advantage during CPV-2 outbreaks because it is time-efficient,less laborious and does not involve the use of sophisticated instruments.展开更多
Listeriosis is caused by Listeria monocytogenes(LM) and is currently considered to be one of the leading food-borne diseases worldwide, with mortality rate of 20%~30%. Currently, detection methods for LM are time-cons...Listeriosis is caused by Listeria monocytogenes(LM) and is currently considered to be one of the leading food-borne diseases worldwide, with mortality rate of 20%~30%. Currently, detection methods for LM are time-consuming with low sensitivity, and delayed detection results. SYTO9 has a high affinity for DNA and exhibits enhanced fluorescence upon binding. Therefore, this study used SYTO9 staining and image processing to develop a rapid loop mediated isothermal amplification(LAMP) detection method for LM. Smartphone was successfully used for detecting the color change in different concentrations of LM. Besides, the optimized LAMP reaction temperature was 63 °C by color identification, and the limit of detection for LM was 6 copies/μL in the green channel. So, the developed method, based on image processing, is simple, sensitive and rapid, which provides a new idea and method for rapid detection of LM and other food-borne bacterial pathogens.展开更多
An electrochemical sensor based on self-made nano-porous pseudo carbon paste electrode(nano-PPCPE)has been successfully developed,and used to detect Cd^2+ and Pb^2+.The experimental results showed that the electrochem...An electrochemical sensor based on self-made nano-porous pseudo carbon paste electrode(nano-PPCPE)has been successfully developed,and used to detect Cd^2+ and Pb^2+.The experimental results showed that the electrochemical performance of nanoPPCPE is evidently better than both glassy carbon electrode(GCE)and pure carbon paste electrode(CPE).Then the prepared nano-PPCPE was applied to detect Cd^2+ and Pb^2+in standard solution,the results showed that the electrodes can quantitatively detect trace Cd^2+ and Pb^2+,which has great significance in electrochemical analysis and detection.The linear ranges between the target ions concentration and the D PASV current were from 0.1-3.0 μmol/L,0.05-4.0 μmol/L for Cd^2+ and Pb^2+,respectively.And the detection limits were 0.0780 μmol/L and 0.0292 μmol/L,respectively.Moreover,the preparation of the nano-PPCPE is cheap,simple and has important practical value.展开更多
Chemiluminescence immunoassay(CLⅠA) has always been a great challenge in detecting cardiac troponin Ⅰ(c Tn Ⅰ) in whole blood samples without centrifugation because of the interference of red blood cells and low sen...Chemiluminescence immunoassay(CLⅠA) has always been a great challenge in detecting cardiac troponin Ⅰ(c Tn Ⅰ) in whole blood samples without centrifugation because of the interference of red blood cells and low sensitivity. Ⅰn this study, the antigens and erythrocytes in the blood were captured by the antibodies immobilized on the magnetic particles, recognized by another biotinconjugated c Tn Ⅰ antibody and detected by streptavidin/acridine aster-conjugated polychloromethylstyrene microspheres(PCMS). After magnetic separation, the supernatant was transferred and measured. No significant difference was noted between the c Tn Ⅰ concentrations of the serum samples,plasma samples and whole blood. The prepared PCMS provided more functional areas to conjugate streptavidin and acridinium ester, so the immunoassay has highly sensitive, the limits of blank at0.012 ng/mL, and functional sensitivity at 0.019 ng/mL with a CV of 20%, and 0.058 ng/mL with a CV of 10%. Total precision of any sample type ranged from 2.62%~5.67%. The assay was linear over the studied range of 0.01-50.00 ng/mL, and no hook effect was found when c Tn Ⅰ concentrations reached 1900 ng/mL. No significant interference was noted with the potential endogenous interfering substances. Compared with the commercial kit(Abbott assay kit), the correlation coefficient was 0.9859. A washing-free CLⅠA was established for the rapid detection of c Tn Ⅰ in human whole blood, using erythrocyte capture antibodies-conjugated magnetic nanoparticles for eliminating the influence of erythrocytes and PCMS for signal amplification, which showed great potential in clinical application.展开更多
基金supported by the National Natural Science Foundation of China(52174247 and 22302066)“Hejian”Innovative Talent Project of Hunan Province(No.2022RC1088)+1 种基金the Hunan Provincial Natural Science Foundation(2023JJ40255)the Scientific Research Foundation of Hunan Provincial Education(22B0599 and 23A0442)。
文摘Hydrogel electrolytes hold great potential in flexible zinc ion supercapacitors(ZICs)due to their high conductivity,good safety,and flexibility.However,freezing of electrolytes at low temperature(subzero)leads to drastic reduction in ionic conductivity and mechanical properties that deteriorates the performance of flexible ZICs.Besides,the mechanical fracture during arbitrary deformations significantly prunes out the lifespan of the flexible device.Herein,a Zn^(2+)and Li^(+)co-doped,polypyrrole-dopamine decorated Sb_(2)S_(3)incorporated,and polyvinyl alcohol/poly(N-(2-hydroxyethyl)acrylamide)double-network hydrogel electrolyte is constructed with favorable mechanical reliability,anti-freezing,and self-healing ability.In addition,it delivers ultra-high ionic conductivity of 8.6 and 3.7 S m^(-1)at 20 and−30°C,respectively,and displays excellent mechanical properties to withstand tensile stress of 1.85 MPa with tensile elongation of 760%,together with fracture energy of 5.14 MJ m^(-3).Notably,the fractured hydrogel electrolyte can recover itself after only 90 s of infrared illumination,while regaining 83%of its tensile strain and almost 100%of its ionic conductivity during−30–60°C.Moreover,ZICs coupled with this hydrogel electrolyte not only show a wide voltage window(up to 2 V),but also provide high energy density of 230 Wh kg^(-1)at power density of 500 W kg^(-1)with a capacity retention of 86.7%after 20,000 cycles under 20°C.Furthermore,the ZICs are able to retain excellent capacity even under various mechanical deformation at−30°C.This contribution will open up new insights into design of advanced wearable flexible electronics with environmental adaptability and long-life span.
基金supported by the National Key Research and Development Program of China(Nos.2017YFA0205301 and 2018YFC1602905)National Natural Science Foundation of China(Nos.62071119,62075098,81902153,61527806)the Open Funding of State Key Laboratory of Oral Diseases(No.SKLOD2022OF05).
文摘Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased body.To address this, primary cells that can be stably passaged were isolated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this study.Next, an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells, and the obtained nucleic acid aptamers were affinity verified at the cellular level.Finally, to answer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers, we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model, and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.
基金funded by the Science and Technology Development Fund,Macao SAR(Nos.0065/2020/A2,SKLQRCM(MUST)-2020-2022)Shenzhen-Hong Kong-Macao Science and Technology Project(Grade c)(No.SGDX20210823104201010).
文摘Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduce the transmission of gastroenteritis.In the study,a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres,and a closed-cassette-based,integrated,reagent-ambient storage,on-site instant detection platform for norovirus GII was constructed using a commercial,fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection,with a sensitivity of 10 copies/μL,with no cross-reactivity with other 5 viruses.For 28 simulated samples,the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction(RT-qPCR)assays after conventional laboratory nucleic acid extraction.The entire process can be finished in about 1 h,which is ideal for immediate rapid detection.
基金the NSFC(Nos.61701176 and 62071119)Macao FDCT(No.0065/2020/A2)+5 种基金Natural Science Foundation of Hunan Province of China(Nos.2022JJ50052,2018JJ3130 and 2020JJ5145)Hunan Key R&D Projects(No.2021SK2003)Nanjing Important Science&Technology Specific Projects(No.2021-11005)2022 Special Project for the Construction of Innovative Provinces to Fight the COVID-19 Outbreak(No.2022SK2115)Open Funding of State Key Laboratory of Oral Diseases(No.SKLOD2022OF05)Shenzhen Innovation and Entrepreneurship Program Innovation and Entrepreneurship Special Project(No.20220624181237005).
文摘The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in late 2019 has negatively affected people's lives and productivity.Because the mode of transmission of SARS-CoV-2 is of great concern,this review discusses the sources of virus aerosols and possible transmission routes.First,we discuss virus aerosol collection methods,including natural sedimentation,solid impact,liquid impact,centrifugal,cyclone and electrostatic adsorption methods.Then,we review common virus aerosol detection methods,including virus culture,metabolic detection,nucleic acid-based detection and immunology-based detection methods.Finally,possible solutions for the detection of SARS-CoV-2 aerosols are introduced.Point-of-care testing has long been a focus of attention.In the near future,the development of an instrument that integrates sampling and output results will enable the real-time,automatic monitoring of patients.
基金Project(51874129)supported by the National Natural Science Foundation of ChinaProjects(2018JJ3115,2019JJ60049)supported by the Science Foundation of Hunan Province,ChinaProjects(19B153,19B158)supported by the Scientific Research Fund of Hunan Provincial Education Department,China。
文摘Novel hydrophilic NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+double-jacket microtubes(DJMTs)with upconversion/downconversion dual-mode luminescence were designed and prepared through epitaxial growth of NaGdF4:Ce^3+,Eu^3+shell onto the NaYF4:Yb^3+,Tm^3+microtube via poly(acrylic acid)(PAA)mediated hydrothermal method.It is demonstrated that PAA ligand played an important role in guiding the direct growth of NaGdF4:Ce^3+,Eu^3+shell onto the surface of NaYF4:Yb^3+,Tm^3+parent microtubes.The growth of NaGdF4:Ce^3+,Eu^3+shell experienced a crystal phase transition fromβ-NaGdF4 andβ-NaYF4 mixture toβ-NaYF4@NaGdF4 composite crystal,and morphology evolution from mixture ofβ-NaGdF4:Ce^3+,Eu^3+nanorods andβ-NaYF4:Yb^3+,Tm^3+microtubes to NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+DJMTs.The formation mechanism of DJMTs was the dissolution−renucleation ofβ-NaGdF4:Ce^3+,Eu^3+nanorods and the growth ofβ-NaGdF4:Ce^3+,Eu^3+shell via the classical Ostwald ripening mechanism.The as-prepared DJMTs could exhibit blue upconversion and red downconversion luminescence,which was further made into environmentally benign luminescent inks for creating highly secured and fluorescent-based anti-counterfeiting patterns via inkjet printing.
基金the financial supports from the National Natural Science Foundation of China (Nos. 51974116, 51874128)the Science Foundation of Hunan Province, China (Nos. 2020JJ4273, 2020JJ5130)。
文摘Palladium-supported cobalt hydroxide(Co(OH)_(2)-Pd) nanoplates were fabricated in an aqueous solution and employed as a catalyst for the reduction of 4-nitrophenol.For the preparation of Co(OH)2-Pd,Pd nanoparticles were anchored on the Co(OH)_(2) nanoplates after the reduction of Na;PdCl;by ascorbic acid in the absence of a stabilizer at room temperature.The observations under transmission and scanning electron microscopy reveal that Pd nanoparticles with a size of 2-5 nm are uniformly dispersed on the surface of the Co(OH)_(2) nanoplates.In catalytic test,the conversion of 4-nitrophenol to 4-aminophenol is completed within 6 min in the presence of Co(OH)_(2)-Pd(1000) nanoplates with2.18 at.% Pd,and the corresponding kinetic constant is 0.0089 s;in the first test.The catalyst retains relatively high activity after several cycles.The results demonstrate that the Co(OH)_(2)-Pd(1000) nanoplates exhibit high catalytic activity toward the reduction of 4-nitrophenol in the presence of NaBH;.
基金supported by the National Natural Science Foundation of China (Nos.62176089,61703152)the Hunan Provincial Natural Science Foundation (Nos.2021JJ30226,2018JJ3134)+1 种基金Scientific Research Foundation of Hunan Provincial Education Department (No.21B0532)Science and Technology Planning Project of Zhuzhou (No.2020015).
文摘Herein,we fabricated a flexible semidry electrode with excellent mechanical performance,satisfactory self-adhesiveness,and low-contact impedance using physical/chemical crosslinked polyvinyl alcohol/polyacrylamide dual-network hydrogels(PVA/PAM DNHs)as an efficient saline reservoir.The resultant PVA/PAM DNHs showed admirable adhesive and compliance to the hairy scalp,facilitating the establishment of a robust electrode/skin interface for biopotential signal transmission.Moreover,the PVA/PAM DNHs steadily released trace saline onto the scalp to achieve the minimized potential drift(1.47±0.39 mV/min)and low electrode–scalp impedance(18.2±8.9 kΩ@10 Hz).More importantly,the application feasibility of real-world brain−computer interfaces(BCIs)was preliminarily validated by 10 participants using two classic BCI paradigms.The mean temporal cross-correlation coefficients between the semidry and wet electrodes in the eyes open/closed and the N200 speller paradigms are 0.919±0.054 and 0.912±0.050,respectively.Both electrodes demonstrate anticipated neuroelectrophysiological responses with similar patterns.This semidry electrode could also effectively capture robust P-QRS-T peaks during electrocardiogram recording.Considering their outstanding advantages of fast setup,user-friendliness,and robust signals,the proposed PVA/PAM DNH-based electrode is a promising alternative to wet electrodes in biopotential signal acquisition.
基金supported by the State Key Basic Research Program of the PRC(No.2014CB744501)the National Key Research and Development Program of China(No.2017YFA0205301)+1 种基金the National Natural Science Foundation of China(Nos.61527806,61471168 and 61871180)Open Funding of State Key Laboratory of Oral Diseases(No.SKLOD2018OF02)
文摘The feature of the surface coating can affect important properties of iron oxide nanoparticles(IONPs), it is therefore critical for further understanding how these materials react to physiological conditions, which is still needed to fully exploit the potential of IONPs for their theranostic applications. In this work, we prepared IONPs which surface were modified with citric acid(CA), chitosan(CS) and folic acid conjugated chitosan(FA-g-CS). respectively. Their physicochemical properties were investigated using FT-IR, TEM,powder XRD, VSM, TGA, DLS and zeta potential. We found that CA-IONP dispersion was composed of monocrystalline particles while CS-IONP and FA-g-CS-IONP were composed of polycrystalline aggregates. All IONPs retained the crystalline structure of magnetite and exhibited the superparamagnetic behavior. Their saturation magnetization decreased with the increase in the amount of their organic coatings. Their drug loading capacities, drug release patterns and in vitro anticancer efficiencies were studied by using doxorubicin(DOX) as a model drug. DOX@CS-IONP and DOX@FA-g-CSIONP exhibited lower drug loading while showing higher water dispersity when compared with DOX@CA-IONP. All IONPs were surface charged and they tended to agglomerate in medium with high pH value and ionic strength. In the presence of chitosan or FA-g-CS coatings, their DOX release rate was slowed down compared with that of DOX@CA-IONP. Unloaded IONPs exhibited nearly no cytotoxicity on both cancer cells and normal cells in the presence of chitosan and FA-g-CS when compared with CA-IONP which presented high cytotoxicity. However, DOX@FA-g-CS-IONP showed significantly cytotoxicity on folate receptors(FRs) positive breast cancer cells while exhibiting nearly no cytotoxicity on FRs negative normal cells. Results presented in this study were valuable to the design and fabrication of IONPs-based system for better theranostic applications.
基金supported by the National Natural Science Foundation of China (Nos. 61901168, 61971187, 61871180, 61571187, 81902153)Zhuzhou Innovative City Construction Project (No. 2020-020)+2 种基金China Postdoctoral Science Foundation (No. 2018M630498)Hunan Urgency Project (No. 2020SK3005)Education Department Outstanding Young Project of Hunan Province (No. 18B299)。
文摘Point-of-care nucleic acid testing(POCNAT) has played an important role in the outbreak of infectious diseases(e.g., COVID-19) over recent years. POCNAT aims to realize the rapid, simple and automatic detection of nucleic acid. Thanks to the development of manufacturing technology, electronic information technology, artificial intelligence technology, and biological information technology in recent years, the development of the POCNAT device has led to significant advancement. Instead of the normal nucleic acid detection methods used in the laboratory, some novel experimental carriers have been applied, such as chips, cartridges and papers. The application of these experimental carriers has realized the automation and integration of nucleic acid detection. The entire process of nucleic acid detection is normally divided into three steps(nucleic acid extraction, target amplification and signal detection). All of the reagents required by the process can be pre-stored on these experimental carriers, without unnecessary manual operation. Furthermore, all of the processes are carried out in this experimental carrier, with the assistance of a specific control device. Although they are complicated to manufacture and precise in design,their application provides a significant step forwards in nucleic acid detection and realizes the integration of nucleic acid detection. This technology has great potential in the field of point-of-care molecular diagnostics in the future. This paper focuses on the relevant content of these experimental carriers.
基金financially supported by the National Natural Science Foundation of China(No.52174247)the Distinguish Young Scientists of Hunan province(No.2022JJ10024)+2 种基金"Hejian"Innovative Talent Project of Hunan Province(No.2022RC1088)the Natural Science Foundation of Hunan Province(Nos.2021JJ30212 and 2021JJ30216)the Scientific Research and Innovation Foundation of Hunan University of Technology(No.CX2209)。
文摘Binary transition metal sulfides are hotly investigated in advanced energy storage devices because of their ultra-high reversible capacity.Nevertheless,the unsatisfied rate capability and cycling stability still hinder their practical application.Herein,hierarchical nanoporous carbon@NiCo_(2)S_(4)(HNCMs@NCS)composites with coreshell flower-like structures were prepared by in situ growing of NiCo_(2)S_(4) nanosheets on HNCMs through a hydro thermal-as sis ted template sacrificial method.Benefiting from a synergistic effect between the NiCo_(2)S_(4)shell with high specific capacity and the HNCMs with unique porous structure,the synthesized flower-like HNCMs@NCS composites exhibit extraordinary electrochemical performances,including a high capacity of 346.9 mAh·g^(-1)at 1 A·g^(-1),superb rate property with86.4%initial capacity at 30 A·g^(-1)and predominant cycle stability with 81.2%capacity retention after 5000 cycles.Furthermore,the resulting HNCMs@NCS cathode was coupled with the chemical-activated HNCMs(AHNCMs)anode to construct a hybrid supercapacitor device.The asfabricated device exhibits superior energy density(49.9 Wh·kg^(-1)at 802 W·kg^(-1))and ultra-high power density(24 kW·kg^(-1)at 29.5 Wh·kg^(-1)).This fascinating result further demonstrates the tremendous prospect of the synthesized HNCMs@NCS composites as high-performance supercapacitor electrode materials.
基金financially supported by Hainan Provincial Natural Science Foundation of China(No.822CXTD514)Hainan Province Science and Technology Special Found(No.ZDYF2022SHFZ123)。
文摘Compared with other types of breast cancer,triple-negative breast cancer(TNBC)has the characteristics of a high degree of malignancy and poor prognosis.Early diagnosis of TNBC through biological markers and timely development of effective treatment methods can reduce its mortality.Many Research experiments have confirmed that some specific mi RNA expression profiles in TNBC can used as markers for early diagnosis.However,detecting the expression profiles of multiple groups of miRNAs according to traditional detection methods is complicated and consumes many samples.To address this issue,we developed a method for high-throughput,high-sensitivity quantitative detection of multiple sets of miRNAs(including mi R-16,mi R-21,mi R-92,mi R-199,and mi R-342)specifically expressed in TNBC by rolling circle amplification(RCA)on fluorescence-encoded microspheres.Through the optimization of reaction system conditions,the developed method showed an extensive linear dynamic range and high sensitivity for all five miRNAs with the lowest limit of detection of 2 fmol/L.Meanwhile,this high-throughput detection method also appeared reasonable specificity.Only in the presence of a specific target miRNA,the fluorescence signal on the correspondingly encoded microspheres is significantly increased,while the fluorescence signal on other non-correspondingly encoded microspheres is almost negligible.Furthermore,this process exhibited good recovery and reproducibility in serum.The advantages of this method allow us to more conveniently obtain the expression profiles of multiple groups of TNBC-associated mi RNAs,which is beneficial for the early detection of TNBC.
基金supported by the grant from the National Natural Science Foundation of China(Nos.61901168,61971187,61871180,82002405)Hunan Key Research and Development Program(No.2021SK2003)+1 种基金Zhuzhou Innovative City Construction Project(No.2020-020)China Postdoctoral Science Foundation(No.2018M630498)。
文摘Enterocytozoon hepatopenaei(EHP)infection has seriously affected prawn culture globally.The symptoms of the infection are not apparent,and traditional detection methods are time consuming and low in accuracy.We developed a new onsite rapid testing device(size 18.8×16.7×6.6 cm^(3))for EHP based on magnesium pyrophosphate precipitation and facilitated by loop mediated isothermal amplification(LAMP).The design and fabrication of the device enables efficient light absorbance.The device has a highly sensitive detector,high-precision thermal controller,and humanized touch screen.The temperature control precision of the device is 0.2-0.3℃ at 60℃,63℃,and 65℃.The coefficients of variation values(CVV)of the luminous power in one channel at light on and off were found to be 0.0097 and 0.0014,respectively,within 1 h.The CVV of the background,luminous power,and values of eight PCR tubes filled with pure water were all less than 5%.In the EHP experiment,eight samples(including seven positive and one negative)confirmed the effectiveness of the device,and four positive and four negative samples verified whether cross-contamination exists.Among them,the rise time of the curve was about 15 min.These results assert that the developed device exhibits enhanced stability and uniformity and has excellent performance with high sensitivity,good specificity,and low testing time.Moreover,the optimal and minimum absorbance range was 555-655 nm for monitoring the production of LAMP.
基金financially supported by the National Natural Science Foundation of China (Nos. 61871180, 61971187, and 61901168)Hunan Provincial Innovation Foundation for Postgraduate (No. CX20211074)Open Funding of State Key Laboratory of Oral Diseases (No. SKLOD2022OF05)。
文摘Phthalates esters(PAEs) are extensively used as additives for polymers in plastic, particularly in polyvinyl chloride(PVC) and polyethylene terephthalate(PET). These compounds are not part of the polymer chains and can be released easily from products and migrate into beverages and foods that come into direct contact, causing environmental and human health impacts. Simple and rapid detection of such substances is of great significance for ensuring environmental food safety and consumer health. At present, optical sensor and electrochemical sensor detection technologies have been applied to PAEs detection due to their advantages, such as simple, rapid, low cost, high sensitivity, simple operation, portability and high specificity. They can make up for the shortcomings of chromatographic detection technology, such as expensive equipment, cumbersome operation, the need for professional and technical personnel, and difficulty in achieving a large number of sample screening objectives. In this paper, research progress on optical sensors and electrochemical sensors for the detection of phthalates in recent ten years is reviewed and discussed. This is helpful to better understand preparation methods for sensors and their detection mechanisms for phthalates. The review will also be used in developing a more effective trace detection sensor for phthalates.
基金supported by the Excellent Youth Fund Project of Henan Natural Science Foundation(Grant No.202300410166)the Major Project of Wenzhou Institute,University of Chinese Academy of Sciences(Grant Nos.WIUCASQD2021004 and WIUCASQD2021035)the National Natural Science Foundation of China(Grant Nos.21504022 and 22202051)。
文摘Up to now, it is always a delicate and challenging task to clean and protect Xuan paper ink painting. Xuan paper is composed of cellulose with a rough surface and easy to absorb dust, which leads to poor cleaning effect and irreversible damage in traditional cleaning process. Hydrogel is one of the most effective tools to clean the artworks. However, in the practice of cleaning Xuan paper, most hydrogels cannot achieve fine cleaning result due to the interfacial adhesion issues. Herein, to protectively and effectively clean Xuan paper, using physical yet strong poly(vinyl alcohol)/poly(N-(2-hydroxyethyl) acrylamide)(PVA/PHEAA)hydrogel with suitable stiffness as the model, the interfacial adhesion between hydrogel and Xuan paper was systematically investigated, and various technologies were used to evaluate the cleaning effect. A critical interfacial adhesive energy(< 4 J/m^(2))is found to achieve the protective and effective cleaning purpose. To the best of our knowledge, this is the first report to estimate the adhesion of hydrogel on the cleaning of paper artwork, which will provide a new viewport in the conservation practice.
基金supported by the NSFC(Nos.61701176 and 62071119)Macao FDCT(No.0065/2020/A2)+4 种基金Natural Science Foundation of Hunan Province of China(Nos.2022JJ50052,2018JJ3130 and 2020JJ5145)Hunan Key R&D Projects(No.2021SK2003)Nanjing Important Science&Technology Specific Projects(No.2021-11005)2022 Special Project for the Construction of Innovative Provinces to Fight the COVID-19 Outbreak(No.2022SK2115)Open Funding of State Key Laboratory of Oral Diseases(No.SKLOD2022OF05)。
文摘The SARS–CoV–2 virus is released from an infectious source(such as a sick person)and adsorbed on aerosols,which can form pathogenic microorganism aerosols,which can affect human health through airborne transmission.Efficient sampling and accurate detection of microorganisms in aerosols are the premise and basis for studying their properties and evaluating their hazard.In this study,we built a set of sub-micron aerosol detection platform,and carried out a simulation experiment on the SARS–CoV–2 aerosol in the air by wet-wall cyclone combined with immunomagnetic nanoparticle adsorption sampling and ddPCR.The feasibility of the system in aerosol detection was verified,and the influencing factors in the detection process were experimentally tested.As a result,the sampling efficiency was 29.77%,and extraction efficiency was 98.57%.The minimum detection limit per unit volume of aerosols was 250 copies(102copies/m L,concentration factor 2.5).
基金supported by the National Natural Science Foundation of China(Nos.82002242,81902153 and 62071119)Natural Science Foundation of Jiangsu Province(No.BK20200135)+3 种基金Hunan Key R&D Projects(No.2021SK2003)Key Project supported by Medical Science and Technology Development Foundation,Nanjing Department of Health(No.YKK20054)Nanjing Important Science&Technology Specific Projects(No.2021-11005)open Funding of State Key Laboratory of Oral Diseases(No.SKLOD2022OF05)。
文摘Extracellular vesicles(EVs)are cell-derived nanosized vesicles widely recognized for their critical roles in various pathophysiological processes.Molecular analysis of EVs is currently being considered an emerging tool for diseases diagnosis.However,the small size and heterogeneity of EVs has staggered the EVs research for diseases diagnosis.DNA nanotechnology enables self-assembly of versatile DNA nanostructures and has shown enormous potential in assisting EVs biosensing.In this review,we briefly introduce the recent advances in DNA nanotechnology approaches for EVs detection.The approaches were categorized based on the dimension of DNA nanostructures.We provide critical evaluation of these approaches,and summarize the pros and cons of specific methods.Further,we discuss the challenges and future perspectives in this field.
基金supported by the National Key Research and Development Program of China (No.2017YFA0205301)National Natural Science Foundation of China (Nos.81902153,61527806 and 81430055)+2 种基金Key Research and Development Project of Jiangsu Province (No.BE2019761)Programs for Changjiang Scholars and Innovative Research Team in University (No.IRT_15R13)open Funding of State Key Laboratory of Oral Diseases (No.SKLOD20190F03)
文摘Canine parvovirus type 2(CPV-2) infection is the most lethal disease of dogs with higher mortality in puppies worldwide.In today’s world,dogs are an integral part of our communities as well as dogs breeding and rearing has become a lucrative business.Therefore,a fast,accurate,portable,and costeffective CPV-2 detection method with the ability for on-site detection is highly desired.In this study,we for the first time proposed a nanosystem for CPV-2 DNA detection with RNA-guided RNA endonuclease Cas13 a,which upon activation results in collateral RNA degradation.We expressed LwCasl3 a in prokaryotic expression system and purified it through nickel column.Activity of Cas13 a was verified by RNA-bound fluorescent group while using a quenched fluorescent probe as signals.Further Cas13 a was combined with Recombinase polymerase amplification(RPA) and T7 transcription to establish molecular detection system termed specific high-sensitivity enzymatic reporter un-locking(SHERLOCK) for sensitive detection of CPV-2 DNA.This nanosystem can detect 100 amol/L CPV-2 DNA within 30 min.The proposed nanosystem exhibited high specificity when tested for CPV-2 and other dog viruses.This CRISPR-Cas13 a mediated sensitive detection approach can be of formidable advantage during CPV-2 outbreaks because it is time-efficient,less laborious and does not involve the use of sophisticated instruments.
基金supported by the grant from the National Natural Science Foundation of China (Nos. 61901168, 8200240581902153)+1 种基金Zhuzhou Innovative City Construction Project(No. 2020–020)China Postdoctoral Science Foundation (No.2018M630498)。
文摘Listeriosis is caused by Listeria monocytogenes(LM) and is currently considered to be one of the leading food-borne diseases worldwide, with mortality rate of 20%~30%. Currently, detection methods for LM are time-consuming with low sensitivity, and delayed detection results. SYTO9 has a high affinity for DNA and exhibits enhanced fluorescence upon binding. Therefore, this study used SYTO9 staining and image processing to develop a rapid loop mediated isothermal amplification(LAMP) detection method for LM. Smartphone was successfully used for detecting the color change in different concentrations of LM. Besides, the optimized LAMP reaction temperature was 63 °C by color identification, and the limit of detection for LM was 6 copies/μL in the green channel. So, the developed method, based on image processing, is simple, sensitive and rapid, which provides a new idea and method for rapid detection of LM and other food-borne bacterial pathogens.
基金the National Key Research and Development Program of China (No.2018YFC1602905)the National Natural Science Foundation of China (Nos.61871180 and 61527806)+1 种基金the Natural Science Foundation of Hunan Province (No.2017JJ2069)Hunan Key Research Project (No.2017SK2174) for the financial supports
文摘An electrochemical sensor based on self-made nano-porous pseudo carbon paste electrode(nano-PPCPE)has been successfully developed,and used to detect Cd^2+ and Pb^2+.The experimental results showed that the electrochemical performance of nanoPPCPE is evidently better than both glassy carbon electrode(GCE)and pure carbon paste electrode(CPE).Then the prepared nano-PPCPE was applied to detect Cd^2+ and Pb^2+in standard solution,the results showed that the electrodes can quantitatively detect trace Cd^2+ and Pb^2+,which has great significance in electrochemical analysis and detection.The linear ranges between the target ions concentration and the D PASV current were from 0.1-3.0 μmol/L,0.05-4.0 μmol/L for Cd^2+ and Pb^2+,respectively.And the detection limits were 0.0780 μmol/L and 0.0292 μmol/L,respectively.Moreover,the preparation of the nano-PPCPE is cheap,simple and has important practical value.
基金financially supported by National Natural Science Foundation of China (Nos.81902153,61871180,62071119 and 61971187)Jiangsu Provincial Key Research and Development Program (Nos.BA2020016 and BE 2018695)。
文摘Chemiluminescence immunoassay(CLⅠA) has always been a great challenge in detecting cardiac troponin Ⅰ(c Tn Ⅰ) in whole blood samples without centrifugation because of the interference of red blood cells and low sensitivity. Ⅰn this study, the antigens and erythrocytes in the blood were captured by the antibodies immobilized on the magnetic particles, recognized by another biotinconjugated c Tn Ⅰ antibody and detected by streptavidin/acridine aster-conjugated polychloromethylstyrene microspheres(PCMS). After magnetic separation, the supernatant was transferred and measured. No significant difference was noted between the c Tn Ⅰ concentrations of the serum samples,plasma samples and whole blood. The prepared PCMS provided more functional areas to conjugate streptavidin and acridinium ester, so the immunoassay has highly sensitive, the limits of blank at0.012 ng/mL, and functional sensitivity at 0.019 ng/mL with a CV of 20%, and 0.058 ng/mL with a CV of 10%. Total precision of any sample type ranged from 2.62%~5.67%. The assay was linear over the studied range of 0.01-50.00 ng/mL, and no hook effect was found when c Tn Ⅰ concentrations reached 1900 ng/mL. No significant interference was noted with the potential endogenous interfering substances. Compared with the commercial kit(Abbott assay kit), the correlation coefficient was 0.9859. A washing-free CLⅠA was established for the rapid detection of c Tn Ⅰ in human whole blood, using erythrocyte capture antibodies-conjugated magnetic nanoparticles for eliminating the influence of erythrocytes and PCMS for signal amplification, which showed great potential in clinical application.
