The potential application of a designed self-assembly peptide CH3CO-Pro-Thr-Phe-CysPhe-Lys-Phe-Glu-Pro-NH2（named as P1） as a carrier of 5-Fluorouracil（5-Fu） for controlled release in vitro was studied. 5-Fluoroura...The potential application of a designed self-assembly peptide CH3CO-Pro-Thr-Phe-CysPhe-Lys-Phe-Glu-Pro-NH2（named as P1） as a carrier of 5-Fluorouracil（5-Fu） for controlled release in vitro was studied. 5-Fluorouracil（5-Fu） was selected as a representative anticancer drug due to its extensive use in treating digestive system cancer and breast cancer. The interaction between P1 and 5-Fu was detected by fluorescent quenching experiments and atomic force microscopy（AFM）. The quenching mechanism of 5-Fu and P1 system was dynamic by performing fluorescent quenching experiments at different temperatures. The thermodynamic analysis demonstrated that the interaction between 5-Fu and P1 was hydrophobic interaction. The complexes prepared by the interaction between peptide and 5-Fu appeared as large granular particles of about 20 nm in height under AFM（denoted as5-Fu-P1）, 24 times larger than the original 5-Fu particles. According to the results, an interaction model was proposed. Furthermore, 5-Fu-P1 complexes exhibited an efficient controlled release of 5-Fu in vitro. The research suggested that P1 might be a candidate carrier for drug delivery, providing a substitution agent for 5-Fu.展开更多
A novel algorithm was designed and implemented to realize the numerical calculation of the solvent reorganization energy for electron transfer reactions, on the basis of nonequilibrium solvation theory and the dielect...A novel algorithm was designed and implemented to realize the numerical calculation of the solvent reorganization energy for electron transfer reactions, on the basis of nonequilibrium solvation theory and the dielectric polarizable continuum model. Applying the procedure to the well-investigated intramoleeular electron transfer in biphenyl-androstane-naphthyl and biphenyl-androstane-phenanthryl systems, the numerical results of solvent reorganization energy were determined to be around 60 k J/mol, in good agreement with experimental data. Koopman＇s theorem was adopted for the calculation of the electron transfer coupling element, associated with the linear reaction coordinate approximation. The values for this quantity obtained are acceptable when compared with experimental results.展开更多
Herpes simplex virus （HSV）, a member of the Herpesviridae family, is a significant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections. Acyclovir （ACV） and related nucleos...Herpes simplex virus （HSV）, a member of the Herpesviridae family, is a significant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections. Acyclovir （ACV） and related nucleoside analogues can successfully treat HSV infections, but the emergence of drug resistance to ACV has created a barrier for the treatment of HSV infections, especially in immunocompromised patients. There is an urgent need to explore new and effective tactics to circumvent drug resistance to HSV. This review summarises the current strategies in the development of new targets （the DNA helicase/primase （H/P） complex）, new types of molecules （nature products） and new antiviral mechanisms （lethal mutagenesis of Janus-type nucleosides） to fight the drug resistance of HSV.展开更多
The reassembly of a half-sequence ionic self-complementarity peptide CH3CO-Pro-Ser-Phe- Cys-Phe-Lys-Phe-Glu-Pro-NH2 was reported, which could self-assemble into stable nanofibers, and formed hydrogel consisting of 〉9...The reassembly of a half-sequence ionic self-complementarity peptide CH3CO-Pro-Ser-Phe- Cys-Phe-Lys-Phe-Glu-Pro-NH2 was reported, which could self-assemble into stable nanofibers, and formed hydrogel consisting of 〉99% water. In this study, the nanofiber scaffold was sonicated by an ultrasonic cell disruptor. The effects of sonication were detected by circular dichroism （CD）, atomic force microscopy （AFM）, and rheology. AFM image illustrated that the sonicated fragments could quickly reassemble into nanofibers, while the morphology was distinguishable from the original one. CD spectrum revealed that the conversion occurred mainly between regular β-strand structure and distorted β-strand structure. Rheological analyses showed that the storage modulus （G＇） of the peptide solution at the 7th day after sonication decreased by nearly 40% compared with the value of the solution before sonication. Finally, a plausible conversion model was proposed to interpret the reassembly process.展开更多
Current CO2 reduction and utilization technologies suffer from high energy consuming. Thus, an energy favourable route is in urgent demanding. CO2 mineralization is theoretically an energy releasing process for CO2 re...Current CO2 reduction and utilization technologies suffer from high energy consuming. Thus, an energy favourable route is in urgent demanding. CO2 mineralization is theoretically an energy releasing process for CO2 reduction and utilization, but an approach to recovery this energy has so far remained elusive. For the first time, here we proposed the principle of harvesting electrical energy directly from CO2 mineralization, and realized an energy output strategz1 for CO2 utilization and reduction via a CO2-mineralization fuel cell （CMFC） system. In this system CO2 and industrial alkaline wastes were used as feedstock, and industrial valuable NaHCO3 was produced concomitantly during the electricity generation. The highest power density of this system reached 5.5 W/m2, higher than many microbial fuel cells. The maximum open circuit voltage reached 0.452 V. Moreo- ver, this system was demonstrated viable to low concentration CO2 （10%） and other carhonation process. Thus, the existing of an energy-generating and environmentally friendly strategy to utilize CO2 as a supplement to the current scenario of CO2 emis- sion control has been demonstrated.展开更多
In Arabidopsis thaliana, STN7 kinase is required for phosphorylation of LHCII and for state transitions. In this paper, a hydrophilic polypeptide, derived from the amino acid sequence of STN7, was conjugated to a carr...In Arabidopsis thaliana, STN7 kinase is required for phosphorylation of LHCII and for state transitions. In this paper, a hydrophilic polypeptide, derived from the amino acid sequence of STN7, was conjugated to a carrier protein, bovine serum albumin (BSA), to obtain the polyclonal antibody. Immunogenicity and specificity of the polyclonal antibody were evaluated by agar gel immunodiffusion (AGID) test and Western blot analysis. The results show that besides the phosphorylation of LHCII proteins, also the expression of STN7 was regulated by temperature conditions. In addition, the change tendency of LHCII proteins phosphorylation was not only coherent with expression of STN7 with respect to increasing temperature, but also closely related to state transitions. These results would provide useful information for studying regulatory mechanism of LHCII proteins phosphorylation and expression of STN7.展开更多
While a great deal of research has focused on the application of full-sequence ionic complementary peptide, detection of the capability of half-sequence ionic complementary peptide such as drug carriers, is rarely rep...While a great deal of research has focused on the application of full-sequence ionic complementary peptide, detection of the capability of half-sequence ionic complementary peptide such as drug carriers, is rarely reported. This paper presents that the half-sequence ionic complementary peptide P9 (AC-Pro- Ser-Phe-Asn-Phe-Lys-Phe-Glu-Pro-NH2) can successfully stabilize a model hydrophobic drug pyrene in the aqueous solution. Soybean lecithin vesicles were used to mimic plasma membranes. Fluorescence data show that the pyrene is presented in the crystalline form when stabilized by P9 solution, and molecularly migrated from its peptide encapsulations into the membrane bilayers when the suspension is mixed with lipidosome vesicles. Slower release was observed when thicker coating was applied onto pyrene, which could be to control the wall thickness coating the cargo, and consequently the release rate. The result indicated that P9, with half-sequence ionic complement, may serve as a hydrophobic compounds carrier.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.51003065 and 81000658)New Teachers’Fund for Doctor Stations,and Ministry of Education(No.20100181120077)
文摘The potential application of a designed self-assembly peptide CH3CO-Pro-Thr-Phe-CysPhe-Lys-Phe-Glu-Pro-NH2（named as P1） as a carrier of 5-Fluorouracil（5-Fu） for controlled release in vitro was studied. 5-Fluorouracil（5-Fu） was selected as a representative anticancer drug due to its extensive use in treating digestive system cancer and breast cancer. The interaction between P1 and 5-Fu was detected by fluorescent quenching experiments and atomic force microscopy（AFM）. The quenching mechanism of 5-Fu and P1 system was dynamic by performing fluorescent quenching experiments at different temperatures. The thermodynamic analysis demonstrated that the interaction between 5-Fu and P1 was hydrophobic interaction. The complexes prepared by the interaction between peptide and 5-Fu appeared as large granular particles of about 20 nm in height under AFM（denoted as5-Fu-P1）, 24 times larger than the original 5-Fu particles. According to the results, an interaction model was proposed. Furthermore, 5-Fu-P1 complexes exhibited an efficient controlled release of 5-Fu in vitro. The research suggested that P1 might be a candidate carrier for drug delivery, providing a substitution agent for 5-Fu.
文摘A novel algorithm was designed and implemented to realize the numerical calculation of the solvent reorganization energy for electron transfer reactions, on the basis of nonequilibrium solvation theory and the dielectric polarizable continuum model. Applying the procedure to the well-investigated intramoleeular electron transfer in biphenyl-androstane-naphthyl and biphenyl-androstane-phenanthryl systems, the numerical results of solvent reorganization energy were determined to be around 60 k J/mol, in good agreement with experimental data. Koopman＇s theorem was adopted for the calculation of the electron transfer coupling element, associated with the linear reaction coordinate approximation. The values for this quantity obtained are acceptable when compared with experimental results.
基金the National Natural Science Foundations of China(document no.:81321002,81500860,81300888)a grant from 111 Project of Ministry of Education,China,for fi nancial support
文摘Herpes simplex virus （HSV）, a member of the Herpesviridae family, is a significant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections. Acyclovir （ACV） and related nucleoside analogues can successfully treat HSV infections, but the emergence of drug resistance to ACV has created a barrier for the treatment of HSV infections, especially in immunocompromised patients. There is an urgent need to explore new and effective tactics to circumvent drug resistance to HSV. This review summarises the current strategies in the development of new targets （the DNA helicase/primase （H/P） complex）, new types of molecules （nature products） and new antiviral mechanisms （lethal mutagenesis of Janus-type nucleosides） to fight the drug resistance of HSV.
基金Funded by the National Natural Science Foundation of China(No.51003065)New Teachers'Fund for Doctor Stations,Ministry of Education(No.20100181120077)
文摘The reassembly of a half-sequence ionic self-complementarity peptide CH3CO-Pro-Ser-Phe- Cys-Phe-Lys-Phe-Glu-Pro-NH2 was reported, which could self-assemble into stable nanofibers, and formed hydrogel consisting of 〉99% water. In this study, the nanofiber scaffold was sonicated by an ultrasonic cell disruptor. The effects of sonication were detected by circular dichroism （CD）, atomic force microscopy （AFM）, and rheology. AFM image illustrated that the sonicated fragments could quickly reassemble into nanofibers, while the morphology was distinguishable from the original one. CD spectrum revealed that the conversion occurred mainly between regular β-strand structure and distorted β-strand structure. Rheological analyses showed that the storage modulus （G＇） of the peptide solution at the 7th day after sonication decreased by nearly 40% compared with the value of the solution before sonication. Finally, a plausible conversion model was proposed to interpret the reassembly process.
基金supported by the National Natural Science Foundation of China(Grant Nos.51254002 and 21336004)the National Basic Research Program of China(Grant No.2013BAC12B03)
文摘Current CO2 reduction and utilization technologies suffer from high energy consuming. Thus, an energy favourable route is in urgent demanding. CO2 mineralization is theoretically an energy releasing process for CO2 reduction and utilization, but an approach to recovery this energy has so far remained elusive. For the first time, here we proposed the principle of harvesting electrical energy directly from CO2 mineralization, and realized an energy output strategz1 for CO2 utilization and reduction via a CO2-mineralization fuel cell （CMFC） system. In this system CO2 and industrial alkaline wastes were used as feedstock, and industrial valuable NaHCO3 was produced concomitantly during the electricity generation. The highest power density of this system reached 5.5 W/m2, higher than many microbial fuel cells. The maximum open circuit voltage reached 0.452 V. Moreo- ver, this system was demonstrated viable to low concentration CO2 （10%） and other carhonation process. Thus, the existing of an energy-generating and environmentally friendly strategy to utilize CO2 as a supplement to the current scenario of CO2 emis- sion control has been demonstrated.
基金the National Natural Sciences Foundation of China (Grant No. 30270124)Doctoral Foundation of Ministry of Education of China (Grant No. 20020610094)+1 种基金Program for New Century Excellent Talents in University (Grant No. NCET-04-0861)Sichuan University Research Grant 985
文摘In Arabidopsis thaliana, STN7 kinase is required for phosphorylation of LHCII and for state transitions. In this paper, a hydrophilic polypeptide, derived from the amino acid sequence of STN7, was conjugated to a carrier protein, bovine serum albumin (BSA), to obtain the polyclonal antibody. Immunogenicity and specificity of the polyclonal antibody were evaluated by agar gel immunodiffusion (AGID) test and Western blot analysis. The results show that besides the phosphorylation of LHCII proteins, also the expression of STN7 was regulated by temperature conditions. In addition, the change tendency of LHCII proteins phosphorylation was not only coherent with expression of STN7 with respect to increasing temperature, but also closely related to state transitions. These results would provide useful information for studying regulatory mechanism of LHCII proteins phosphorylation and expression of STN7.
基金Supported by the National ‘985 Project’ of Ministry of Education of China
文摘While a great deal of research has focused on the application of full-sequence ionic complementary peptide, detection of the capability of half-sequence ionic complementary peptide such as drug carriers, is rarely reported. This paper presents that the half-sequence ionic complementary peptide P9 (AC-Pro- Ser-Phe-Asn-Phe-Lys-Phe-Glu-Pro-NH2) can successfully stabilize a model hydrophobic drug pyrene in the aqueous solution. Soybean lecithin vesicles were used to mimic plasma membranes. Fluorescence data show that the pyrene is presented in the crystalline form when stabilized by P9 solution, and molecularly migrated from its peptide encapsulations into the membrane bilayers when the suspension is mixed with lipidosome vesicles. Slower release was observed when thicker coating was applied onto pyrene, which could be to control the wall thickness coating the cargo, and consequently the release rate. The result indicated that P9, with half-sequence ionic complement, may serve as a hydrophobic compounds carrier.
