Some proteins perform their biological functions by changing their material states through liquid-liquid phase separation.Upon phase separation,the protein condenses into a concentrated liquid phase and sometimes into...Some proteins perform their biological functions by changing their material states through liquid-liquid phase separation.Upon phase separation,the protein condenses into a concentrated liquid phase and sometimes into a gel phase,changing its dynamic properties and intermolecular interactions,thereby regulating cellular functions.Although the biological significance of this phenomenon has been widely recognized by researchers,there is still a lack of a comprehensive understanding of the structural and dynamic properties of the protein in the condensed phase.In this phase,molecules usually contain domains with varied dynamic properties and undergo intermediate exchanges.Magic angle spinning(MAS)solid-state NMR(SSNMR)experiments are very powerful in studying rigid protein polymers such as amyloid.The incorporation of solution-like experiments into SSNMR and the development of J-coupling based MAS SSNMR techniques extend its ability to study partially mobile segments of proteins in a condensed liquid or gel phase which are not visible by solution NMR or dipolar-coupling based SSNMR.Therefore,it has been applied in studying protein condensation and has provided very important information that is hard to obtain by other techniques.展开更多
Zeolite-based metal nanoparticles(NPs)catalysts have shown promising applications in fuel production,petro-and fine-chemical manufacture,biomass refinery,and so on.Modulation of hydroxyl groups on the zeolite surface ...Zeolite-based metal nanoparticles(NPs)catalysts have shown promising applications in fuel production,petro-and fine-chemical manufacture,biomass refinery,and so on.Modulation of hydroxyl groups on the zeolite surface has proven to be effective in boosting catalytic reactions over metal-zeolite catalysts.For example,modifying the wettability of ZSM-5 and titanium silicalite-1(TS-1)crystals by surface hydrophobization drastically enhances catalytic performance of zeolites encapsulated AuPd[1]and Pt[2]NPs in methane oxidation and aldehydes/ketones hydrogenation,respectively.In fact,two kinds of hydroxyl groups are present in zeolite materials,which refers to bridged hydroxyls[Si-O(H)-Al]and silanols[Si-O(H)].Both hydroxyl groups are defects of zeolite framework structures,which provides anchor points in dispersing metals[3].The former hydroxyl group is tunable to control acidity by adjusting Si/Al ratio in zeolite,whereas the role of silanols is not fully understood[4],particularly in the dispersion of metal NPs.展开更多
基金supported by the National Natural Science Foundation of China(No.32171185,No.31770790)the National Key R&D Program of China(No.2017YFA0504804).
文摘Some proteins perform their biological functions by changing their material states through liquid-liquid phase separation.Upon phase separation,the protein condenses into a concentrated liquid phase and sometimes into a gel phase,changing its dynamic properties and intermolecular interactions,thereby regulating cellular functions.Although the biological significance of this phenomenon has been widely recognized by researchers,there is still a lack of a comprehensive understanding of the structural and dynamic properties of the protein in the condensed phase.In this phase,molecules usually contain domains with varied dynamic properties and undergo intermediate exchanges.Magic angle spinning(MAS)solid-state NMR(SSNMR)experiments are very powerful in studying rigid protein polymers such as amyloid.The incorporation of solution-like experiments into SSNMR and the development of J-coupling based MAS SSNMR techniques extend its ability to study partially mobile segments of proteins in a condensed liquid or gel phase which are not visible by solution NMR or dipolar-coupling based SSNMR.Therefore,it has been applied in studying protein condensation and has provided very important information that is hard to obtain by other techniques.
基金supported by the National Key R&D Program of China(No.2022YFE0116000,2021 YFA1502600)the National Natural Science Foundation of China(No.21902180).
文摘Zeolite-based metal nanoparticles(NPs)catalysts have shown promising applications in fuel production,petro-and fine-chemical manufacture,biomass refinery,and so on.Modulation of hydroxyl groups on the zeolite surface has proven to be effective in boosting catalytic reactions over metal-zeolite catalysts.For example,modifying the wettability of ZSM-5 and titanium silicalite-1(TS-1)crystals by surface hydrophobization drastically enhances catalytic performance of zeolites encapsulated AuPd[1]and Pt[2]NPs in methane oxidation and aldehydes/ketones hydrogenation,respectively.In fact,two kinds of hydroxyl groups are present in zeolite materials,which refers to bridged hydroxyls[Si-O(H)-Al]and silanols[Si-O(H)].Both hydroxyl groups are defects of zeolite framework structures,which provides anchor points in dispersing metals[3].The former hydroxyl group is tunable to control acidity by adjusting Si/Al ratio in zeolite,whereas the role of silanols is not fully understood[4],particularly in the dispersion of metal NPs.
