Objective: To observe the effect of electroacupuncture (EA) on expression of p53 protein in cerebral cortex of senile rats with global cerebral ischemia/reperfusion (IR) injury and to explore its mechanism. Methods: T...Objective: To observe the effect of electroacupuncture (EA) on expression of p53 protein in cerebral cortex of senile rats with global cerebral ischemia/reperfusion (IR) injury and to explore its mechanism. Methods: The cerebral IR injury rat model was established referring to Pulsinelli 4-vessel occlusion method. Thirty-six SD rats were randomly and evenly divided into the control group, the IR group and the IR plus EA (IR-EA) group. The animals in the control group were subjected to electrocauterization of vertebral arteries in bilateral flank orifice alone with the general carotid arteries unoccluded. To rats in the IR-EA group, immediately and 24h, 48h, 72h after cerebral IR, EA treatment on bilateral acupoint 'Zusanli' (ST36) was applied once a day, lasting for 60 minutes. After the final treatment, all the rats were sacrificed and their brains were taken to examine p53 protein expression by the immunohistochemical method. Results: Cells with positive p53 immunoreactivity in the cerebral cortex of rats in the IR group was significantly higher than that in the control group ( P<0. 05), while that in the IR-EA group was significantly lower than that in the IR group ( P<0. 05). Conclusion: EA could remarkably reduce expression of p53 protein in the cerebral cortex of senile rats with global cerebral IR injury, which might be one of the means for EA to inhibit neuronal ap-optosis after cerebral IR injury.展开更多
Trigeminal Neuralgia is often found in female between 40-60 years old, marked by sudden severe pain on the face (a feeling of knife cutting, electric shock), and it is characterized by sudden, paroxysmal and periodica...Trigeminal Neuralgia is often found in female between 40-60 years old, marked by sudden severe pain on the face (a feeling of knife cutting, electric shock), and it is characterized by sudden, paroxysmal and periodical attack and triggered by touch). The attack usually lasts for 1 to 2 minutes with different intervals. The patient’s life quality can be seriously affected. It is mainly treated with orally taken drugs in western internal department but without satisfactory result, and with local closing therapy in surgical department. The problem may reoccur half a year later in most patients complicated with facial paralysis, so western medicine is not likely to be accepted by the patients. The author has tried to apply abdominal acupuncture in the treatment of 25 cases of trigeminal neuralgia with good effect. The result is as follows.展开更多
Objective： To evaluate the efficacy of oral Chinese medicine （CM） in comparison with donepezil, a cholinesterase inhibitor （ChEI）, for the treatment of Alzheimer＇s disease （AD）. Methods： Randomized controlled...Objective： To evaluate the efficacy of oral Chinese medicine （CM） in comparison with donepezil, a cholinesterase inhibitor （ChEI）, for the treatment of Alzheimer＇s disease （AD）. Methods： Randomized controlled trials （RCTs） have been searched, and the effect of CM compared with donepezil in AD has been investigated. An electronic search of MEDLINE, Excerpta Medica Database （EMBASE）, Cochrane Library, Chinese Biological Medicine Database （CBMdisc）, and China National Knowledge Infrastructure （CNKI） to identify articles in English and Chinese from the inception of the database until October 18, 2015. A modified Jadad score （7-points） to judge the methodological quality of studies, comprehensive meta-analysis was performed with Cochrane Collaboration Revman 5.3. Dichotomous data were analyzed by relative risk （RR） with a 95% confidence interval （CI）, while continuous variables were analyzed by using mean differences （MD） with 95% CI for effect size. Results： Six studies involving 596 AD patients through Jadad assessment with low bias were included in the meta-analysis. No significant difference was observed in cognitive improvement and daily abilities of patients using the Mini Mental State Examination （MMSE） （MD： 0.69, 95% CI： -0.17 to 1.56） and Activities of Daily Living （ADL） scale （MD： 0.94, 95% CI： -1.54 to 3.43）. There were no significant differences in status of illness or MD for mUd-moderate AD patients at 24 weeks （MD： 0.62, 95% CI： -2.99 to 4.23） and 48 weeks （MD： -0.73, 95% CI： -5.02 to 3.56）. Severe AD patients were also assessed at 24 weeks （MD： 3.13, 95% CI： -6.92 to 13.18） and 48 weeks （MD： 4.23, 95% CI： -6.38 to 14.84）. Furthermore, compared with donepezil, Xin （Heart）-regulating CM and Shen （Kidney）-tonifying groups were observed （MD： -1.50, 95% CI： -3.08 to 0.08; MD： -1.92, 95% CI： -3.50 to -0.33; respectively）. CM had fewer side effects in AD patients. Conclusion： Compared with donepezil, oral CM showed no significant difference in effectiveness in AD patients, and more evidence is needed to verify the findings.展开更多
基金This item was supported by Fund on Opening Subject of Key Laboratory in Jiangsu Province (No.K9842)
文摘Objective: To observe the effect of electroacupuncture (EA) on expression of p53 protein in cerebral cortex of senile rats with global cerebral ischemia/reperfusion (IR) injury and to explore its mechanism. Methods: The cerebral IR injury rat model was established referring to Pulsinelli 4-vessel occlusion method. Thirty-six SD rats were randomly and evenly divided into the control group, the IR group and the IR plus EA (IR-EA) group. The animals in the control group were subjected to electrocauterization of vertebral arteries in bilateral flank orifice alone with the general carotid arteries unoccluded. To rats in the IR-EA group, immediately and 24h, 48h, 72h after cerebral IR, EA treatment on bilateral acupoint 'Zusanli' (ST36) was applied once a day, lasting for 60 minutes. After the final treatment, all the rats were sacrificed and their brains were taken to examine p53 protein expression by the immunohistochemical method. Results: Cells with positive p53 immunoreactivity in the cerebral cortex of rats in the IR group was significantly higher than that in the control group ( P<0. 05), while that in the IR-EA group was significantly lower than that in the IR group ( P<0. 05). Conclusion: EA could remarkably reduce expression of p53 protein in the cerebral cortex of senile rats with global cerebral IR injury, which might be one of the means for EA to inhibit neuronal ap-optosis after cerebral IR injury.
文摘Trigeminal Neuralgia is often found in female between 40-60 years old, marked by sudden severe pain on the face (a feeling of knife cutting, electric shock), and it is characterized by sudden, paroxysmal and periodical attack and triggered by touch). The attack usually lasts for 1 to 2 minutes with different intervals. The patient’s life quality can be seriously affected. It is mainly treated with orally taken drugs in western internal department but without satisfactory result, and with local closing therapy in surgical department. The problem may reoccur half a year later in most patients complicated with facial paralysis, so western medicine is not likely to be accepted by the patients. The author has tried to apply abdominal acupuncture in the treatment of 25 cases of trigeminal neuralgia with good effect. The result is as follows.
基金Supported by the National Natural Science Foundation of China,Youth Found Project(No.81503450)
文摘Objective： To evaluate the efficacy of oral Chinese medicine （CM） in comparison with donepezil, a cholinesterase inhibitor （ChEI）, for the treatment of Alzheimer＇s disease （AD）. Methods： Randomized controlled trials （RCTs） have been searched, and the effect of CM compared with donepezil in AD has been investigated. An electronic search of MEDLINE, Excerpta Medica Database （EMBASE）, Cochrane Library, Chinese Biological Medicine Database （CBMdisc）, and China National Knowledge Infrastructure （CNKI） to identify articles in English and Chinese from the inception of the database until October 18, 2015. A modified Jadad score （7-points） to judge the methodological quality of studies, comprehensive meta-analysis was performed with Cochrane Collaboration Revman 5.3. Dichotomous data were analyzed by relative risk （RR） with a 95% confidence interval （CI）, while continuous variables were analyzed by using mean differences （MD） with 95% CI for effect size. Results： Six studies involving 596 AD patients through Jadad assessment with low bias were included in the meta-analysis. No significant difference was observed in cognitive improvement and daily abilities of patients using the Mini Mental State Examination （MMSE） （MD： 0.69, 95% CI： -0.17 to 1.56） and Activities of Daily Living （ADL） scale （MD： 0.94, 95% CI： -1.54 to 3.43）. There were no significant differences in status of illness or MD for mUd-moderate AD patients at 24 weeks （MD： 0.62, 95% CI： -2.99 to 4.23） and 48 weeks （MD： -0.73, 95% CI： -5.02 to 3.56）. Severe AD patients were also assessed at 24 weeks （MD： 3.13, 95% CI： -6.92 to 13.18） and 48 weeks （MD： 4.23, 95% CI： -6.38 to 14.84）. Furthermore, compared with donepezil, Xin （Heart）-regulating CM and Shen （Kidney）-tonifying groups were observed （MD： -1.50, 95% CI： -3.08 to 0.08; MD： -1.92, 95% CI： -3.50 to -0.33; respectively）. CM had fewer side effects in AD patients. Conclusion： Compared with donepezil, oral CM showed no significant difference in effectiveness in AD patients, and more evidence is needed to verify the findings.
