Purpose:To report a rare case of Mantle cell lymphoma in lacrimal gland and review of the literature Case report:.We report a case of a 59-year-old female who presented with an upper eyelid mass in the right eye for 3...Purpose:To report a rare case of Mantle cell lymphoma in lacrimal gland and review of the literature Case report:.We report a case of a 59-year-old female who presented with an upper eyelid mass in the right eye for 3months, without pain and irrigation. A computerized tomography(CT) scan showed a mass in the bilateral lacrimal gland region, more significant in right eye. The patient underwent a lacrimal gland mass excision surgery and diagnosis of mantle cell lymphoma by histopathology..Immunochemistry for CD20, CD79 a, CD5, and Cyclin D1 was positive. She was recommended to the Shantou cancer hospital for chemotherapy.Conclusion:.Mantle cell lymphoma is a rare type of malignant lymphoma,.over expressing CD5 and cyclin D1 antigens,which distinguishes it from other B cell lymphomas.展开更多
AIM: To investigate the difference of macular thickness measurements between Stratus optical coherence tomography(OCT) and Cirrus OCT(Carl Zeiss Meditec,Dublin, CA, USA) in the same myopic patient and to develop a con...AIM: To investigate the difference of macular thickness measurements between Stratus optical coherence tomography(OCT) and Cirrus OCT(Carl Zeiss Meditec,Dublin, CA, USA) in the same myopic patient and to develop a conversion equation to interchange macular thickness obtained with these two OCT devices.·METHODS: Eighty-nine healthy Chinese adults with spherical equivalent(SE) ranging from-1.13 D to-9.63 D were recruited. The macular thickness was measured by Cirrus OCT and Stratus OCT. The correlation between macular thickness and axial length and the agreement between two OCT measurements were evaluated. A formula was generated to interchange macular thickness obtained with two OCT devices.·RESULTS: Average macular thickness measured with Stratus OCT(r =-0.280, P =0.008) and Cirrus OCT(r =-0.224,P =0.034) were found to be negatively correlated with axial length. No statistically significant correlation was found between axial length and central subfield macular thickness(CMT) measured with Stratus OCT(r =0.191,P =0.073) and Cirrus OCT(r =0.169, P =0.113). The mean CMT measured with Cirrus OCT was 53.63 ±7.94 μm thicker than with Stratus OCT. The formula CMTCirrus OCT=78.328 +0.874 ×CMTStratus OCTwas generated to interchange macular thickness obtained with two OCT devices.· CONCLUSION: Macular thickness measured with Cirrus OCT were thicker than with Stratus OCT in myopic eyes. A formula can be used to interchange macular thickness measured with two OCT devices in myopic eyes. Studies with different OCT devices and larger samples are warranted to enable the comparison of macular values measured with different OCT devices.展开更多
Background: To investigate early change of corneal aberrations after 2.8-mm superior incision phacoemulsification.Methods: This study comprised 80 eyes of 75 patients. All the patients underwent phacoemulsification wi...Background: To investigate early change of corneal aberrations after 2.8-mm superior incision phacoemulsification.Methods: This study comprised 80 eyes of 75 patients. All the patients underwent phacoemulsification with monofocal foldable intraocular lens(IOLs) implanted through a 2.8-mm superior corneal incision. The anterior corneal wavefront aberrations for the 6.0-mm pupillary diameter was measured by i Trace wavefront aberrometer(Tracey Technologies, Inc.) preoperatively and 1 month postoperatively. Changes of root mean square(RMS) values of Z(3,-3), Z(3, 3), Z(3,-1), Z(3, 1), and Z(4, 0) and total high order aberration(HOA) were evaluated.Results: The uncorrected and corrected visual acuities improve significantly(P<0.001). No significant postoperative changes were observed in spherical aberration(P=0.652). Significant changes in vertical coma and vertical trefoil(0.005±0.214 vs.-0.049±0.242, P=0.037;-0.141±0.222 vs.-0.258±0.359, P=0.001; separately). However, the total HOAs increased after cataract surgery(0.567±0.161 vs. 0.688±0.343, P<0.001).Conclusions: Corneal vertical coma and vertical trefoil changes significantly in 2.8mm superior corneal incision phacoemulsification cataract surgery. In addition, those had a trend to negative direction.展开更多
Short Retraction Notice The paper does not meet the standards of “Chinese Medicine ". This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE&#...Short Retraction Notice The paper does not meet the standards of “Chinese Medicine ". This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. The aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. Editor guiding this retraction: Prof. Maythem Saeed (EiC of CM) The full retraction notice in PDF is preceding the original paper, which is marked "RETRACTED".展开更多
This study was designed to compare optical biometry measurements and predicted refraction in cataract patients with high myopia [axial length(AL) ≥26 mm] using OA-2000 and IOL Master 500. Ocular biometry measurements...This study was designed to compare optical biometry measurements and predicted refraction in cataract patients with high myopia [axial length(AL) ≥26 mm] using OA-2000 and IOL Master 500. Ocular biometry measurements were performed using both biometers before surgery. Uneventful cataract surgery was performed in all patients. Postoperative manifest refraction was obtained 3 wk after surgery or later. A total of 67 eyes were examined. The AL, keratometry(K), and anterior chamber depth(ACD) of the two biometers showed excellent agreement. Predicted errors were similar and a strong positive correlation was observed(r=0.909). Out of 21 eyes(31.34%) with unsuccessful AL readings using the IOL Master 500, 20 eyes of them could be measured using OA-2000. Therefore, the biometric parameters measured by the two biometers showed good agreement. However, OA-2000 had a lower AL measurement failure rate.展开更多
Currently, thyroid-associated ophthalmopathy (TAO) lacks effective treatment due to our lack of clarity in its immunopathogenesis. Orbital fibroblasts play a key role in altering inflammation and immune response in TA...Currently, thyroid-associated ophthalmopathy (TAO) lacks effective treatment due to our lack of clarity in its immunopathogenesis. Orbital fibroblasts play a key role in altering inflammation and immune response in TAO, and are considered as the key target and effector cells in its pathogenesis. The orbit infiltrating CD34+ fibrocytes add on to the process by expressing high levels of autoantigens and inflammatory cytokines, while also differentiating into myofibroblasts or adipocytes. This review focuses on the role of orbital fibroblasts and CD34+ fibrocytes in the pathogenesis of TAO, highlighting the basis of emerging treatments.展开更多
AIM: To assess the repeatability and reproducibility of Tonolab tonometer in rats with high intraocular pressure(IOP) and evaluate its ability to detect IOP changes in rats with general anaesthesia.METHODS: Left eyes ...AIM: To assess the repeatability and reproducibility of Tonolab tonometer in rats with high intraocular pressure(IOP) and evaluate its ability to detect IOP changes in rats with general anaesthesia.METHODS: Left eyes of adult Fischer rats(F344) were photocoagulated by 532 nm diode laser to induce high IOP. Hypertensive eyes of 30 conscious rats were randomly chosen to measure IOP on a single occasion.Two observers independently and alternately undertook IOP measurements consecutively for three times using the same Tonolab tonometer blind to the other observer's IOP measurements. The within subject standard deviation(Sw), coefficient of variation(CVw)(100 ×Sw/overall mean), and intraclass correlation coefficient(ICC) were calculated to evaluate intra-observer repeatability. Inter-observer difference was analysed by using 95% limits of agreement described by Bland-Altman and paired sample t- test. Also, another13 normal F344 rats were intraperitoneally administrated with ketamine/xylazine or chloral hydrate, and IOPs of both eyes were measured by a single operator once every 5min until animals came to conscious. IOPs at various time points were compared by using one-way ANOVAs.RESULTS: Mean IOP was 35.58 mm Hg(range 17.33 to65.33 mm Hg). For intraobserver repeatability, the Sw,CVw and ICC of high IOP for two observers were 5.20 mm Hg/3.41 mm Hg, 9.98% /8.08% and 0.820/0.928 respectively.The inter-observer difference was 14.76% ±19.76% of the mean IOP of two observers, with a 95% limits of agreement-23.97% to 53.50%, and the difference between mean IOP of these two observers was statistically significant(P =0.001). IOPs dropped slightly during the first 15 min post-aneathesia, with a IOP change between0.17 and 1.17 mm Hg. IOPs changed from basline of11.75 ±2.05 mm Hg(n =12) to 8.75 ±1.06 mm Hg 20 min post-anesthesia(P =0.001), and this hypotensivecondition persisted until 80 min post-anesthesia. CONCLUSION: In this sample of hypertensive rats,Tonolab measurements demonstrated high levels of intraobserver repeatability, however, its interobserver reproducibility was poor. Longitudinal changes of IOP caused by genral anaesthesia can be sensitively detected by Tonolab. So we suggested that measurements of IOP using Tonolab are best measured by a single observer,and it could be included in experimental glaucoma.展开更多
Background: Corneal ectasia is a serious complication after laser in situ keratomileusis(LASIK). Ideally, patients at risk of ectasia should be identified prior to laser as unsuitable for LASIK, however, at present, t...Background: Corneal ectasia is a serious complication after laser in situ keratomileusis(LASIK). Ideally, patients at risk of ectasia should be identified prior to laser as unsuitable for LASIK, however, at present, there is no absolute test, system, or marker that can unequivocally identify patients at risk of developing ectasia. It has been suggested that changes in the forward protrusion of the posterior cornea or posterior corneal elevation(PCE) may be a key to the early detection of ectasia after LASIK. The purpose of this study was to examine the long term changes of the PCE after myopic LASIK using the ORBSCAN~ IIz(Bausch & Lomb, Rochester, USA) and to evaluate the contributory preoperative factors to PCE changes.Methods: This was a retrospective longitudinal case series. Forty-two eyes of 23 consecutive myopic patients who underwent uneventful LASIK surgery 6 years ago were recalled for a postoperative follow-up examination using the ORBSCAN~ IIz to determine the elevation changes to the posterior corneal surface from the preoperative measurements. A forward shift of the posterior surface was given a negative value. Correlation analyses and forward stepwise regression analyses were performed to evaluate the effect of each of the preoperative parameters on the changes in PCE. Statistical and graphical analyses were performed.Results: There was no statistical difference between the mean best-fit sphere(BFS) pre-LASIK and 6 years postop(P=0.25). Forty-two post-LASIK eyes had a mean posterior displacement of -9.38±9.84 μm(range, 12 to-31 μm) 6 years after LASIK. Forward stepwise multiple linear regression analysis indicated that the ablation spherical equivalent(ASE) was the only indicator of the forward shift of the posterior cornea after LASIK.Conclusions: The present study identified a significant forward shift of the posterior cornea 6 years after LASIK. The ASE was the most significant prognostic determinant for forward protrusion of the posterior cornea after LASIK.展开更多
Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2...Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2 inhibition can promote retinal ganglion cell survival and axonal regeneration in rats after optic nerve injury.To investigate the underlying mechanism,in the current study we increased the intraocular pressure of adult rats to 75 mmHg for 2 hours and then administered a casein kinase-2 inhibitor(4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole)by intravitreal injection.We found that intravitreal injection of 4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole promoted retinal ganglion cell survival and reduced the number of infiltrating macrophages.Transcriptomic analysis showed that the mitogen activated protein kinase signaling pathway was involved in the response to intraocular pressure elevation but was not modulated by the casein kinase-2 inhibitors.Furthermore,casein kinase-2 inhibition downregulated the expression of genes(Cck,Htrsa,Nef1,Htrlb,Prph,Chat,Slc18a3,Slc5a7,Scn1b,Crybb2,Tsga10ip,and Vstm21)involved in intraocular pressure elevation.Our data indicate that inhibition of casein kinase-2 can enhance retinal ganglion cell survival in rats after acute intraocular pressure elevation via macrophage inactivation.展开更多
The prospects of stem cell therapy for retinal ganglion cell(RGC)degeneration in human:RGC degeneration is a common pathologic cause of glaucoma and optic neuropathies,which are the leading cause of irreversible blind...The prospects of stem cell therapy for retinal ganglion cell(RGC)degeneration in human:RGC degeneration is a common pathologic cause of glaucoma and optic neuropathies,which are the leading cause of irreversible blindness and visual impairment in developed countries,currently affecting more than 100 million people worldwide.Intraocular pressure lowering can slow down glaucoma progression in a proportion of patients.Also,there is still no effective therapy for optic neuropathies.Besides,the degenerated RGCs in glaucoma cannot展开更多
Both inflammation and anti-inflammation are involved in the protection of retinal cells.Antagonists of the hypothalamic growth hormone-releasing hormone receptor(GHRHR)have been shown to possess potent anti-inflammato...Both inflammation and anti-inflammation are involved in the protection of retinal cells.Antagonists of the hypothalamic growth hormone-releasing hormone receptor(GHRHR)have been shown to possess potent anti-inflammatory properties in experimental disease models of various organs,some with systemic complications.Such effects are also found in ocular inflammatory and neurologic injury studies.In experimental models of mice and rats,both growth hormone-releasing hormone receptor agonists and antagonists may alleviate death of ocular neural cells under certain experimental conditions.This review explores the properties of growth hormone-releasing hormone receptor agonists and antagonists that lead to its protection against inflammatory responses induced by extrinsic agents or neurologic injures in ocular animal models.展开更多
Inflammation is a critical pathophysiological process that modulates neuronal survival in the central nervous system after disease or injury.However,the effects and mechanisms of macrophage activation on neuronal surv...Inflammation is a critical pathophysiological process that modulates neuronal survival in the central nervous system after disease or injury.However,the effects and mechanisms of macrophage activation on neuronal survival remain unclear.In the present study,we co-cultured adult Fischer rat retinas with primary peritoneal macrophages or zymosan-treated peritoneal macrophages for 7 days.Immunofluorescence analysis revealed that peritoneal macrophages reduced retinal ganglion cell survival and neurite outgrowth in the retinal explant compared with the control group.The addition of zymosan to peritoneal macrophages attenuated the survival and neurite outgrowth of retinal ganglion cells.Conditioned media from peritoneal macrophages also reduced retinal ganglion cell survival and neurite outgrowth.This result suggests that secretions from peritoneal macrophages mediate the inhibitory effects of these macrophages.In addition,increased inflammationand oxidation-related gene expression may be related to the enhanced retinal ganglion cell degeneration caused by zymosan activation.In summary,this study revealed that primary rat peritoneal macrophages attenuated retinal ganglion cell survival and neurite outgrowth,and that macrophage activation further aggravated retinal ganglion cell degeneration.This study was approved by the Animal Ethics Committee of the Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong,Shantou,Guangdong Province,China,on March 11,2014(approval no.EC20140311(2)-P01).展开更多
Dear Editor,We are writing to present an uncommon case of a45-year-old woman with bilateral proliferative diabetic retinopathy (PDR) and ocular albinism (OA).Albinism is a group of inherited disorders of tyrosinase ac...Dear Editor,We are writing to present an uncommon case of a45-year-old woman with bilateral proliferative diabetic retinopathy (PDR) and ocular albinism (OA).Albinism is a group of inherited disorders of tyrosinase activities and melanin biosynthesis resulting in little or no production of the pigment melanin。展开更多
Dear Editor,We are writing to present a rare episcleral osseous choristomas in a 12-year-old girl.Choristomas are congenital benign mass composed of normal tissue located in an abnormal site[1].Ocular choristomas can ...Dear Editor,We are writing to present a rare episcleral osseous choristomas in a 12-year-old girl.Choristomas are congenital benign mass composed of normal tissue located in an abnormal site[1].Ocular choristomas can be categorized as limbaldermoid,lipodermoid.展开更多
Dear Editor,We are writing to present a bilateral uveal coloboma concurrent with retinal detachment(RD)in a 16-year-old girl.Colobomas are rare,congenital and potentially blinding malformations caused by incomplete cl...Dear Editor,We are writing to present a bilateral uveal coloboma concurrent with retinal detachment(RD)in a 16-year-old girl.Colobomas are rare,congenital and potentially blinding malformations caused by incomplete closure of embryonic optic fissure[1].展开更多
Background:To report a new simplified surgical technique to manage small iris coloboma or traumatic iris defect.Methods:A new surgical technique in which simplified pupilloplasty technique through only a clear corneal...Background:To report a new simplified surgical technique to manage small iris coloboma or traumatic iris defect.Methods:A new surgical technique in which simplified pupilloplasty technique through only a clear corneal paracentesis to manage the iris coloboma or traumatic iris defect within the 120°range was designed.A retrospective revision of the medical records of patients treated with this technique between the years 2013 and 2016 was made.Six eyes of six patients with iris coloboma or traumatic iris defect treated with this new technique were included.Results:All the operated eyes quickly recovered with central round pupil,negligible complications,inessential symptoms of photophobia and glare,and mild inflammation after a median follow-up time of 22 months(range:6-34 months).Conclusions:The simplified pupilloplasty technique presented here could be a good alternative for the management of small iris coloboma or traumatic iris defect.展开更多
文摘Purpose:To report a rare case of Mantle cell lymphoma in lacrimal gland and review of the literature Case report:.We report a case of a 59-year-old female who presented with an upper eyelid mass in the right eye for 3months, without pain and irrigation. A computerized tomography(CT) scan showed a mass in the bilateral lacrimal gland region, more significant in right eye. The patient underwent a lacrimal gland mass excision surgery and diagnosis of mantle cell lymphoma by histopathology..Immunochemistry for CD20, CD79 a, CD5, and Cyclin D1 was positive. She was recommended to the Shantou cancer hospital for chemotherapy.Conclusion:.Mantle cell lymphoma is a rare type of malignant lymphoma,.over expressing CD5 and cyclin D1 antigens,which distinguishes it from other B cell lymphomas.
基金Supported by Shantou University Medical College Clinical Research Improve Projects in 2014 (No.201417)
文摘AIM: To investigate the difference of macular thickness measurements between Stratus optical coherence tomography(OCT) and Cirrus OCT(Carl Zeiss Meditec,Dublin, CA, USA) in the same myopic patient and to develop a conversion equation to interchange macular thickness obtained with these two OCT devices.·METHODS: Eighty-nine healthy Chinese adults with spherical equivalent(SE) ranging from-1.13 D to-9.63 D were recruited. The macular thickness was measured by Cirrus OCT and Stratus OCT. The correlation between macular thickness and axial length and the agreement between two OCT measurements were evaluated. A formula was generated to interchange macular thickness obtained with two OCT devices.·RESULTS: Average macular thickness measured with Stratus OCT(r =-0.280, P =0.008) and Cirrus OCT(r =-0.224,P =0.034) were found to be negatively correlated with axial length. No statistically significant correlation was found between axial length and central subfield macular thickness(CMT) measured with Stratus OCT(r =0.191,P =0.073) and Cirrus OCT(r =0.169, P =0.113). The mean CMT measured with Cirrus OCT was 53.63 ±7.94 μm thicker than with Stratus OCT. The formula CMTCirrus OCT=78.328 +0.874 ×CMTStratus OCTwas generated to interchange macular thickness obtained with two OCT devices.· CONCLUSION: Macular thickness measured with Cirrus OCT were thicker than with Stratus OCT in myopic eyes. A formula can be used to interchange macular thickness measured with two OCT devices in myopic eyes. Studies with different OCT devices and larger samples are warranted to enable the comparison of macular values measured with different OCT devices.
文摘Background: To investigate early change of corneal aberrations after 2.8-mm superior incision phacoemulsification.Methods: This study comprised 80 eyes of 75 patients. All the patients underwent phacoemulsification with monofocal foldable intraocular lens(IOLs) implanted through a 2.8-mm superior corneal incision. The anterior corneal wavefront aberrations for the 6.0-mm pupillary diameter was measured by i Trace wavefront aberrometer(Tracey Technologies, Inc.) preoperatively and 1 month postoperatively. Changes of root mean square(RMS) values of Z(3,-3), Z(3, 3), Z(3,-1), Z(3, 1), and Z(4, 0) and total high order aberration(HOA) were evaluated.Results: The uncorrected and corrected visual acuities improve significantly(P<0.001). No significant postoperative changes were observed in spherical aberration(P=0.652). Significant changes in vertical coma and vertical trefoil(0.005±0.214 vs.-0.049±0.242, P=0.037;-0.141±0.222 vs.-0.258±0.359, P=0.001; separately). However, the total HOAs increased after cataract surgery(0.567±0.161 vs. 0.688±0.343, P<0.001).Conclusions: Corneal vertical coma and vertical trefoil changes significantly in 2.8mm superior corneal incision phacoemulsification cataract surgery. In addition, those had a trend to negative direction.
文摘Short Retraction Notice The paper does not meet the standards of “Chinese Medicine ". This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. The aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. Editor guiding this retraction: Prof. Maythem Saeed (EiC of CM) The full retraction notice in PDF is preceding the original paper, which is marked "RETRACTED".
基金Supported by Natural Science Foundation of Guangdong Province (No.2015A030310504)Guangdong Medical Science and Technology Research Fund (No. B2018142)The Shantou City Science and Technology Project, China (No.170829211930487)
文摘This study was designed to compare optical biometry measurements and predicted refraction in cataract patients with high myopia [axial length(AL) ≥26 mm] using OA-2000 and IOL Master 500. Ocular biometry measurements were performed using both biometers before surgery. Uneventful cataract surgery was performed in all patients. Postoperative manifest refraction was obtained 3 wk after surgery or later. A total of 67 eyes were examined. The AL, keratometry(K), and anterior chamber depth(ACD) of the two biometers showed excellent agreement. Predicted errors were similar and a strong positive correlation was observed(r=0.909). Out of 21 eyes(31.34%) with unsuccessful AL readings using the IOL Master 500, 20 eyes of them could be measured using OA-2000. Therefore, the biometric parameters measured by the two biometers showed good agreement. However, OA-2000 had a lower AL measurement failure rate.
基金Supported by National Natural Science Foundation of China(No.81200719)China Postdoctoral Science Foundation(No.2013M543579+1 种基金 No.2014T71013)Key Specialized Projects in Nanjing(No.SZDZK2016008)
文摘Currently, thyroid-associated ophthalmopathy (TAO) lacks effective treatment due to our lack of clarity in its immunopathogenesis. Orbital fibroblasts play a key role in altering inflammation and immune response in TAO, and are considered as the key target and effector cells in its pathogenesis. The orbit infiltrating CD34+ fibrocytes add on to the process by expressing high levels of autoantigens and inflammatory cytokines, while also differentiating into myofibroblasts or adipocytes. This review focuses on the role of orbital fibroblasts and CD34+ fibrocytes in the pathogenesis of TAO, highlighting the basis of emerging treatments.
基金Supported by National Natural Science Foundation of China (No.30672282)
文摘AIM: To assess the repeatability and reproducibility of Tonolab tonometer in rats with high intraocular pressure(IOP) and evaluate its ability to detect IOP changes in rats with general anaesthesia.METHODS: Left eyes of adult Fischer rats(F344) were photocoagulated by 532 nm diode laser to induce high IOP. Hypertensive eyes of 30 conscious rats were randomly chosen to measure IOP on a single occasion.Two observers independently and alternately undertook IOP measurements consecutively for three times using the same Tonolab tonometer blind to the other observer's IOP measurements. The within subject standard deviation(Sw), coefficient of variation(CVw)(100 ×Sw/overall mean), and intraclass correlation coefficient(ICC) were calculated to evaluate intra-observer repeatability. Inter-observer difference was analysed by using 95% limits of agreement described by Bland-Altman and paired sample t- test. Also, another13 normal F344 rats were intraperitoneally administrated with ketamine/xylazine or chloral hydrate, and IOPs of both eyes were measured by a single operator once every 5min until animals came to conscious. IOPs at various time points were compared by using one-way ANOVAs.RESULTS: Mean IOP was 35.58 mm Hg(range 17.33 to65.33 mm Hg). For intraobserver repeatability, the Sw,CVw and ICC of high IOP for two observers were 5.20 mm Hg/3.41 mm Hg, 9.98% /8.08% and 0.820/0.928 respectively.The inter-observer difference was 14.76% ±19.76% of the mean IOP of two observers, with a 95% limits of agreement-23.97% to 53.50%, and the difference between mean IOP of these two observers was statistically significant(P =0.001). IOPs dropped slightly during the first 15 min post-aneathesia, with a IOP change between0.17 and 1.17 mm Hg. IOPs changed from basline of11.75 ±2.05 mm Hg(n =12) to 8.75 ±1.06 mm Hg 20 min post-anesthesia(P =0.001), and this hypotensivecondition persisted until 80 min post-anesthesia. CONCLUSION: In this sample of hypertensive rats,Tonolab measurements demonstrated high levels of intraobserver repeatability, however, its interobserver reproducibility was poor. Longitudinal changes of IOP caused by genral anaesthesia can be sensitively detected by Tonolab. So we suggested that measurements of IOP using Tonolab are best measured by a single observer,and it could be included in experimental glaucoma.
文摘Background: Corneal ectasia is a serious complication after laser in situ keratomileusis(LASIK). Ideally, patients at risk of ectasia should be identified prior to laser as unsuitable for LASIK, however, at present, there is no absolute test, system, or marker that can unequivocally identify patients at risk of developing ectasia. It has been suggested that changes in the forward protrusion of the posterior cornea or posterior corneal elevation(PCE) may be a key to the early detection of ectasia after LASIK. The purpose of this study was to examine the long term changes of the PCE after myopic LASIK using the ORBSCAN~ IIz(Bausch & Lomb, Rochester, USA) and to evaluate the contributory preoperative factors to PCE changes.Methods: This was a retrospective longitudinal case series. Forty-two eyes of 23 consecutive myopic patients who underwent uneventful LASIK surgery 6 years ago were recalled for a postoperative follow-up examination using the ORBSCAN~ IIz to determine the elevation changes to the posterior corneal surface from the preoperative measurements. A forward shift of the posterior surface was given a negative value. Correlation analyses and forward stepwise regression analyses were performed to evaluate the effect of each of the preoperative parameters on the changes in PCE. Statistical and graphical analyses were performed.Results: There was no statistical difference between the mean best-fit sphere(BFS) pre-LASIK and 6 years postop(P=0.25). Forty-two post-LASIK eyes had a mean posterior displacement of -9.38±9.84 μm(range, 12 to-31 μm) 6 years after LASIK. Forward stepwise multiple linear regression analysis indicated that the ablation spherical equivalent(ASE) was the only indicator of the forward shift of the posterior cornea after LASIK.Conclusions: The present study identified a significant forward shift of the posterior cornea 6 years after LASIK. The ASE was the most significant prognostic determinant for forward protrusion of the posterior cornea after LASIK.
基金supported by the National Natural Science Foundation of China,Nos.81570849,81100931the Natural Science Foundation of Guangdong Province of China,Nos.2015A030313446,2020A1515011413(all to LPC).
文摘Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2 inhibition can promote retinal ganglion cell survival and axonal regeneration in rats after optic nerve injury.To investigate the underlying mechanism,in the current study we increased the intraocular pressure of adult rats to 75 mmHg for 2 hours and then administered a casein kinase-2 inhibitor(4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole)by intravitreal injection.We found that intravitreal injection of 4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole promoted retinal ganglion cell survival and reduced the number of infiltrating macrophages.Transcriptomic analysis showed that the mitogen activated protein kinase signaling pathway was involved in the response to intraocular pressure elevation but was not modulated by the casein kinase-2 inhibitors.Furthermore,casein kinase-2 inhibition downregulated the expression of genes(Cck,Htrsa,Nef1,Htrlb,Prph,Chat,Slc18a3,Slc5a7,Scn1b,Crybb2,Tsga10ip,and Vstm21)involved in intraocular pressure elevation.Our data indicate that inhibition of casein kinase-2 can enhance retinal ganglion cell survival in rats after acute intraocular pressure elevation via macrophage inactivation.
基金supported in part by the National Natural Science Foundation of China(81570849 and 81470636)Research Fund for the Doctoral Program of Higher Education of China(20114402120007)Natural Science Foundation of Guangdong Province(2015A030313446),China
文摘The prospects of stem cell therapy for retinal ganglion cell(RGC)degeneration in human:RGC degeneration is a common pathologic cause of glaucoma and optic neuropathies,which are the leading cause of irreversible blindness and visual impairment in developed countries,currently affecting more than 100 million people worldwide.Intraocular pressure lowering can slow down glaucoma progression in a proportion of patients.Also,there is still no effective therapy for optic neuropathies.Besides,the degenerated RGCs in glaucoma cannot
基金supported by the National Natural Science Foundation of China(81570849 to LPC)Joint Regional Basic Science and Applied Basic Science Research Fund of Guangdong Province(2019 A1515110685 to TKN)+4 种基金Special Fund for Chinese Medicine Development of Guangdong Province(20202089 to TKN)the Natural Science Foundation of Guangdong Province(2020 A1515010415 to LPC)an internal grant from Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong KongGrant for Key Disciplinary Project of Clinical Medicine under the Guangdong High-level University Development Program, ChinaThe Chinese University of Hong Kong Direct Grant(2020.067 to WKC)
文摘Both inflammation and anti-inflammation are involved in the protection of retinal cells.Antagonists of the hypothalamic growth hormone-releasing hormone receptor(GHRHR)have been shown to possess potent anti-inflammatory properties in experimental disease models of various organs,some with systemic complications.Such effects are also found in ocular inflammatory and neurologic injury studies.In experimental models of mice and rats,both growth hormone-releasing hormone receptor agonists and antagonists may alleviate death of ocular neural cells under certain experimental conditions.This review explores the properties of growth hormone-releasing hormone receptor agonists and antagonists that lead to its protection against inflammatory responses induced by extrinsic agents or neurologic injures in ocular animal models.
基金supported by the National Natural Science Foundation of China,No.81570849(to LPC)the Natural Science Foundation of Guangdong Province of China,No.2020A1515010415(to LPC)+1 种基金the Special Fund for Chinese Medicine Development of Guangdong Province of China,No.20202089(to TKN)the Grant for Key Disciplinary Project of Clinical Medicine under the Guangdong High-Level University Development Program,No.002-18119101.
文摘Inflammation is a critical pathophysiological process that modulates neuronal survival in the central nervous system after disease or injury.However,the effects and mechanisms of macrophage activation on neuronal survival remain unclear.In the present study,we co-cultured adult Fischer rat retinas with primary peritoneal macrophages or zymosan-treated peritoneal macrophages for 7 days.Immunofluorescence analysis revealed that peritoneal macrophages reduced retinal ganglion cell survival and neurite outgrowth in the retinal explant compared with the control group.The addition of zymosan to peritoneal macrophages attenuated the survival and neurite outgrowth of retinal ganglion cells.Conditioned media from peritoneal macrophages also reduced retinal ganglion cell survival and neurite outgrowth.This result suggests that secretions from peritoneal macrophages mediate the inhibitory effects of these macrophages.In addition,increased inflammationand oxidation-related gene expression may be related to the enhanced retinal ganglion cell degeneration caused by zymosan activation.In summary,this study revealed that primary rat peritoneal macrophages attenuated retinal ganglion cell survival and neurite outgrowth,and that macrophage activation further aggravated retinal ganglion cell degeneration.This study was approved by the Animal Ethics Committee of the Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong,Shantou,Guangdong Province,China,on March 11,2014(approval no.EC20140311(2)-P01).
文摘Dear Editor,We are writing to present an uncommon case of a45-year-old woman with bilateral proliferative diabetic retinopathy (PDR) and ocular albinism (OA).Albinism is a group of inherited disorders of tyrosinase activities and melanin biosynthesis resulting in little or no production of the pigment melanin。
文摘Dear Editor,We are writing to present a rare episcleral osseous choristomas in a 12-year-old girl.Choristomas are congenital benign mass composed of normal tissue located in an abnormal site[1].Ocular choristomas can be categorized as limbaldermoid,lipodermoid.
文摘Dear Editor,We are writing to present a bilateral uveal coloboma concurrent with retinal detachment(RD)in a 16-year-old girl.Colobomas are rare,congenital and potentially blinding malformations caused by incomplete closure of embryonic optic fissure[1].
基金This work was supported by Medical Research Fund Project of Guangdong Province(A2017486).
文摘Background:To report a new simplified surgical technique to manage small iris coloboma or traumatic iris defect.Methods:A new surgical technique in which simplified pupilloplasty technique through only a clear corneal paracentesis to manage the iris coloboma or traumatic iris defect within the 120°range was designed.A retrospective revision of the medical records of patients treated with this technique between the years 2013 and 2016 was made.Six eyes of six patients with iris coloboma or traumatic iris defect treated with this new technique were included.Results:All the operated eyes quickly recovered with central round pupil,negligible complications,inessential symptoms of photophobia and glare,and mild inflammation after a median follow-up time of 22 months(range:6-34 months).Conclusions:The simplified pupilloplasty technique presented here could be a good alternative for the management of small iris coloboma or traumatic iris defect.
