Objective:Panax notoginseng saponins (PNS),extracted from rhizome of the herb Radix et Rhizoma Notoginseng (Panax notoginseng (Burk.) F.H.Chen),was recently discovered to have beneficial effects against neurological d...Objective:Panax notoginseng saponins (PNS),extracted from rhizome of the herb Radix et Rhizoma Notoginseng (Panax notoginseng (Burk.) F.H.Chen),was recently discovered to have beneficial effects against neurological damage.This study investigated the effects of PNS on cerebral ischemia and elucidated the molecular mechanisms underlying these effects.Methods:Middle cerebral artery occlusion rats were treated with PNS (3.6 mg/100 g or 7.2 mg/100 g per day) for 7 days,the gene of LINGO-1 was measured and the expression of protein synaptophysin,postsynaptic density protein 95,LINGO-1 and p-EGFR/p-PI3K/p-AKT were investigated.The weight and mNSS score of Sprague-Dawley rats in each group were recorded every day during the 7 days.Results:PNS promoted middle cerebral artery occlusion rats' weight and the recovery of neural function.PNS significantly decreased ischemia-induced LINGO-1 protein expression.PNS also elevated EGFR/PI3K/AKT phosphorylation levels.Conclusion:PNS promoted cerebral recovery from ischemic injury by accelerating synapse reconstruction and inhibiting the neuron growth negative regulatory protein LINGO-1 and activating the epidermal growth factor receptor (EGFR)/PI3K/AKT signaling pathway in vivo.展开更多
Objective:To investigate the anti-cancer effects and mechanism of modified Liangfu granule(MLFG)in BGC-823 gastric cancer cells.Methods:The drug serum was extracted from abdominal aorta of Wistar rats treated by cyclo...Objective:To investigate the anti-cancer effects and mechanism of modified Liangfu granule(MLFG)in BGC-823 gastric cancer cells.Methods:The drug serum was extracted from abdominal aorta of Wistar rats treated by cyclophosphamide,dioscin,MLFG(high-,medium-,and low-dose),respectively.MTS assay was performed to detect the effect of different concentrations of MLFG and dioscin on cell growth.The effect of MLFG on cellular apoptosis was detected by flow cytometry.Western blot was used to examine Bcl-2 and Akt in BGC-823 cells treated with MLFG.Quantitative real-time polymerase chain reaction assay was performed to determine the expression level of caspase-3,E2F1 and E2F3 genes in cells treated by MLFG-and dioscin-containing serum.Results:MLFG-and dioscin-containing serum inhibited the cell proliferation of BGC-823 cells.MLFG induced gastric carcinoma cell apoptosis and inhibited the expression of Bcl-2 and Akt in a dosedependent manner.MLFG also significantly induced the gene expression of caspase-3 and downregulated E2F1 and E2F3 gene expression.Conclusion:The effect of MLFG and dioscin on inhibiting cell proliferation and induction of apoptosis of gastric cancer cells might be related to the regulation of Bcl-2 and Akt proteins and the expression of caspase-3,E2F1 and E2F3 genes.展开更多
基金funding from the National Natural Science Foundation of China(Grant Nos.81573926 and 81173235).
文摘Objective:Panax notoginseng saponins (PNS),extracted from rhizome of the herb Radix et Rhizoma Notoginseng (Panax notoginseng (Burk.) F.H.Chen),was recently discovered to have beneficial effects against neurological damage.This study investigated the effects of PNS on cerebral ischemia and elucidated the molecular mechanisms underlying these effects.Methods:Middle cerebral artery occlusion rats were treated with PNS (3.6 mg/100 g or 7.2 mg/100 g per day) for 7 days,the gene of LINGO-1 was measured and the expression of protein synaptophysin,postsynaptic density protein 95,LINGO-1 and p-EGFR/p-PI3K/p-AKT were investigated.The weight and mNSS score of Sprague-Dawley rats in each group were recorded every day during the 7 days.Results:PNS promoted middle cerebral artery occlusion rats' weight and the recovery of neural function.PNS significantly decreased ischemia-induced LINGO-1 protein expression.PNS also elevated EGFR/PI3K/AKT phosphorylation levels.Conclusion:PNS promoted cerebral recovery from ischemic injury by accelerating synapse reconstruction and inhibiting the neuron growth negative regulatory protein LINGO-1 and activating the epidermal growth factor receptor (EGFR)/PI3K/AKT signaling pathway in vivo.
基金the National Natural Science Foundation of China(81573959)the Capital Health Research and Development of Special Fund(2016-1-4171).
文摘Objective:To investigate the anti-cancer effects and mechanism of modified Liangfu granule(MLFG)in BGC-823 gastric cancer cells.Methods:The drug serum was extracted from abdominal aorta of Wistar rats treated by cyclophosphamide,dioscin,MLFG(high-,medium-,and low-dose),respectively.MTS assay was performed to detect the effect of different concentrations of MLFG and dioscin on cell growth.The effect of MLFG on cellular apoptosis was detected by flow cytometry.Western blot was used to examine Bcl-2 and Akt in BGC-823 cells treated with MLFG.Quantitative real-time polymerase chain reaction assay was performed to determine the expression level of caspase-3,E2F1 and E2F3 genes in cells treated by MLFG-and dioscin-containing serum.Results:MLFG-and dioscin-containing serum inhibited the cell proliferation of BGC-823 cells.MLFG induced gastric carcinoma cell apoptosis and inhibited the expression of Bcl-2 and Akt in a dosedependent manner.MLFG also significantly induced the gene expression of caspase-3 and downregulated E2F1 and E2F3 gene expression.Conclusion:The effect of MLFG and dioscin on inhibiting cell proliferation and induction of apoptosis of gastric cancer cells might be related to the regulation of Bcl-2 and Akt proteins and the expression of caspase-3,E2F1 and E2F3 genes.
