A high-efficiency mode of high-low seedbed cultivation(HLSC)has been listed as the main agricultural technology to increase land utilization ratio and grain yield in Shandong Province,China.However,limited information...A high-efficiency mode of high-low seedbed cultivation(HLSC)has been listed as the main agricultural technology to increase land utilization ratio and grain yield in Shandong Province,China.However,limited information is available on the optimized water and nitrogen management for yield formation,especially the grain-filling process,under HLSC mode.A three-year field experiment with four nitrogen rates and three irrigation rates of HLSC was conducted to reveal the response of grain-filling parameters,grain weight percentage of spike weight(GPS),spike moisture content(SMC),and winter wheat yield to water and nitrogen rates.The four nitrogen rates were N1(360 kg ha^(-1) pure N),N2(300 kg ha^(-1) pure N),N3(240 kg ha^(-1) pure N),and N4(180 kg ha^(-1) pure N),respectively,and the three irrigation quotas were W1(120 mm),W2(90 mm),and W3(60 mm),respectively.Results showed that the determinate growth function generally performed well in simulating the temporal dynamics of grain weight(0.989<R^(2)<0.999,where R2 is the determination coefficient).The occurrence time of maximum filling rate(T_(max))and active grain-filling period(AGP)increased with the increase in the water or nitrogen rate,whereas the average grain-filling rate(G_(mean))had a decreasing trend.The final 1,000-grain weight(FTGW)increased and then decreased with the increase in the nitrogen rates and increased with the increase in the irrigation rates.The GPS and SMC had a highly significant quadratic polynomial relationship with grain weight and days after anthesis.Nitrogen,irrigation,and year significantly affected the T_(max),AGP,G_(mean),and FTGW.Particularly,the AGP and FTGW were insignificantly different between high seedbed(HLSC-H)and low seedbed(HLSC-L)across the water and nitrogen levels.Moreover,the moderate water and nitrogen supply was more beneficial for grain yield,as well as for spike number and grain number per hectare.The principal component analysis indicated that combining 240-300 kg N ha^(-1) and 90^(-1)20 mm irrigation quota could improve grain-filling efficiency and yield for the HLSC-cultivated winter wheat.展开更多
Excessive application of nitrogen (N) fertilizer is the main cause of N loss and poor use efficiency in winter wheat (Triticum aestivum L.) production in the North China Plain (NCP).Drip fertigation is considered to b...Excessive application of nitrogen (N) fertilizer is the main cause of N loss and poor use efficiency in winter wheat (Triticum aestivum L.) production in the North China Plain (NCP).Drip fertigation is considered to be an effective method for improving N use efficiency and reducing losses,while the performance of drip fertigation in winter wheat is limited by poor N scheduling.A two-year field experiment was conducted to evaluate the growth,development and yield of drip-fertigated winter wheat under different split urea (46%N,240 kg ha^(-1)) applications.The six treatments consisted of five fertigation N application scheduling programs and one slow-release fertilizer (SRF) application.The five N scheduling treatments were N0–100 (0%at sowing and 100%at jointing/booting),N25–75 (25%at sowing and 75%at jointing and booting),N50–50(50%at sowing and 50%at jointing/booting),N75–25 (75%at sowing and 25 at jointing/booting),and N100–0 (100%at sowing and 0%at jointing/booting).The SRF (43%N,240 kg ha^(-1)) was only used as fertilizer at sowing.Split N application significantly (P<0.05) affected wheat grain yield,yield components,aboveground biomass (ABM),water use efficiency(WUE) and nitrogen partial factor productivity (NPFP).The N50–50 and SRF treatments respectively had the highest yield(8.84 and 8.85 t ha^(-1)),ABM (20.67 and 20.83 t ha^(-1)),WUE (2.28 and 2.17 kg m^(-3)) and NPFP (36.82 and 36.88 kg kg^(-1)).This work provided substantial evidence that urea-N applied in equal splits between basal and topdressing doses compete economically with the highly expensive SRF for fertilization of winter wheat crops.Although the single-dose SRF could reduce labor costs involved with the traditional method of manual spreading,the drip fertigation system used in this study with the N50–50 treatment provides an option for farmers to maintain wheat production in the NCP.展开更多
Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the m...Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the minituber production after transplanting. With 100 μmol m^-2s^-1 total photosynthetic photon flux density(PPFD), a light spectrum study of 100% red light emitting diodes(LEDs) light spectrum(RR), 100% blue LEDs light spectrum(BB), 65% red+35% blue LEDs light spectrum(RB), and 45% red+35% blue+20% green LEDs light spectrum(RBG) providing illumination at the in vitro cultured stage of potato plantlets for 4 weeks using fluorescent lamp as control(CK) was performed to investigate the effects of LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro as well as the minituber yield after 2 months transplanting in the greenhouse. Compared to CK, RB and RBG promoted the growth of potato plantlets in vitro with increased stem diameter, plantlet fresh weight, plantlet dry weight, and health index. Furthermore, BB induced the greatest stem diameter as well as the highest health index in potato plantlets in vitro. Root activity, soluble protein, and free amino acid were also significantly enhanced by BB, whereas carbohydrates were improved by RR. In addition, thickness of leaf, palisade parenchyma and spongy parenchyma was significantly increased by BB and RBG. Chloroplasts under BB and RBG showed well-developed grana thylakoid and stroma thylakoid. Unexpectedly, distinct upper epidermis with greatest thickness was induced and palisade parenchyma and spongy parenchyma were arranged neatly in RR. After transplanting in the greenhouse for 2 months, potato plantlets in vitro from BB, RB, and RBG produced high percentage of large size tuber. BB improved fresh and dry weights of the biggest tuber but decreased tuber number per plantlet. In addition, RBG increased tuber number as well as tuber fresh and dry weight slightly. Our results suggested monochromatic blue LEDs as well as combined red, blue or/and green LEDs light spectrum were superior to fluorescent lamp spectrum in micro-propagation of potato plantlets. Therefore, the application of RBG was suitable;BB and RB could be used as alternatives.展开更多
Increasing plant density can increase cereal crop yields. However, the physiological and anatomical mechanisms of grain yield increase at high plant densities in maize-based intercropping systems are not well understo...Increasing plant density can increase cereal crop yields. However, the physiological and anatomical mechanisms of grain yield increase at high plant densities in maize-based intercropping systems are not well understood. A two-year field experiment was conducted in 2018 and 2019 to investigate grain yield, photosynthetic characteristics, stomatal traits, and leaf anatomy of maize plants in an intercropping system with high plant densities. Two cropping patterns(monocropping and intercropping) and three plant densities(D1, 78,000 plants ha^(-1);D2, 103,500 plants ha^(-1);D3, 129,000 plants ha;were arranged in a randomized block design. Increasing maize plant density significantly increased maize yield, and intercropping gave a significant yield advantage over monocropping under the same plant density. Intercropping combined with high plant density increased the leaf area and SPAD value of maize,increasing the photosynthesis rates after the harvest of pea. At the twelfth leaf stage, the stomatal density and stomatal area of intercrops combined with medium plant density increased by respectively 10.5%and 18.4% relative to their values for the corresponding density of monocrops. Although leaf thickness of maize was reduced by increasing plant density, the chloroplast number and grana lamella number were higher in intercropping than in monocropping under different plant densities. These positive changes in leaf anatomy resulted in increased photosynthesis, suggesting a physiological basis for the increase in grain yield.展开更多
With fresh winter jujube from the southern region of Xinjiang as the object of study, the method for detecting pesticide residues on winter jujube surface by near-infrared spectroscopy （NIR） combined with successive...With fresh winter jujube from the southern region of Xinjiang as the object of study, the method for detecting pesticide residues on winter jujube surface by near-infrared spectroscopy （NIR） combined with successive projections algorithm （SPA） and partial least squares （PLS） was investigated. The absorbance information of winter jujube sample surface was obtained through NIR technology, for the building of a full-wave band PLS model of fresh winter jujube sample sprayed with different concentrations of pesticide （taking chlorpyrifos as an example） as well as an SPA-PLS model which was built with the characteristic wavelengths extracted with SPA as the input variables for PLS, and the prediction precision of the two kinds of models was compared. The model built with the five characteristic waveslengths extected by SPA method only used the variables 0.32% of all the variables in the full wave band, but its accuracy and precision were better than the model built with the full wave band. It is feasible to build a model for different concentrations of chlorpyrifos on fresh winter jujube surfarce by NIR technology combined with SPA and PLS, and SPA method could simplify the complexity of the model and improve the precision and stablity of the model.展开更多
Greenhousing is a technique to bridge season gap in vegetable production and has been widely used worldwide. Calculation of water requirement of crops grown in greenhouse and determination of their irrigation schedule...Greenhousing is a technique to bridge season gap in vegetable production and has been widely used worldwide. Calculation of water requirement of crops grown in greenhouse and determination of their irrigation schedules in arid and semi-arid regions are essential for greenhouse maintenance and have thus attracted increased attention over the past decades. The most common method used in the literature to estimate crop evapotranspiration（ET） is the Penman-Monteith（PM） formula. When applied to greenhouse, however, it often uses canopy resistance instead of surface resistance. It is understood that the surface resistance in greenhouse is the result of a combined effect of canopy restriction and soil-surface restriction to water vapor flow, and the relative dominance of one restriction over another depends on crop canopy. In this paper, we developed a surface resistance model in a way similar to two parallel resistances in an electrical circuit to account for both restrictions. Also, considering that wind speed in greenhouse is normally rather small, we compared three methods available in the literature to calculate the aerodynamic resistance, which are the r_a^1 method proposed by Perrier（1975a, b）, the r_a^2 method proposed by Thom and Oliver（1977）, and the r_a^3 method proposed by Zhang and Lemeu（1992）. We validated the model against ET of tomatoes in a greenhouse measured from sap flow system combined with micro-lysimeter in 2015 and with weighing lysimeter in 2016. The results showed that the proposed surface resistance model improved the accuracy of the PM model, especially when the leaf area index was low and the greenhouse was being irrigated. We also found that the aerodynamic resistance calculated from the r_a^1 and r_a^3 methods is applicable to the greenhouse although the latter is slightly more accurate than the former. The proposed surface resistance model, together with the r_a^3 method for aerodynamic resistance, offers an improved approach to estimate ET in greenhouse using the PM formula.展开更多
[Objective] The paper was to explore a method for detecting chlorpyrifos residues in red Fuji apple. [Method] The original spectral data of apple samples sprayed with different volume fractions of chlorpyrifos were co...[Objective] The paper was to explore a method for detecting chlorpyrifos residues in red Fuji apple. [Method] The original spectral data of apple samples sprayed with different volume fractions of chlorpyrifos were collected using near infrared spectrometer at the band of 4 000-10 000 cm^（-1）. The original spectra were pre-treated by a variety of methods, and partial least squares（PLS） model was established for predictive analysis. [Result] Near infrared spectrum showed sensitivity to apple samples sprayed with different volume fractions of chlorpyrifos, but had low precision on pesticide-free samples. Data of blank control group were further eliminated for modeling prediction. The results showed that the results were the best when pre-treated with first derivative（FD）： R=0.987 9; the square error of prediction（SEP） was 0.173 6; the root-mean-square error of cross-validation（RMSECV） was 0.120 5; and the precision was 0.923 4. [Conclusion] Near infrared spectrum can better predict chlorpyrifos residue, providing a new method for detecting chlorpyrifos residues in Akesu red Fuji apple.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD1900802)the China Agriculture Research System of MOF and MARA(CARS-03-19)+2 种基金the National Natural Science Foundation of China(51879267)the Central Public-interest Scientific Institution Basal Research Fund,China(IFI2023-13)the Agricultural Science and Technology Innovation Program(ASTIP),Chinese Academy of Agricultural Sciences。
文摘A high-efficiency mode of high-low seedbed cultivation(HLSC)has been listed as the main agricultural technology to increase land utilization ratio and grain yield in Shandong Province,China.However,limited information is available on the optimized water and nitrogen management for yield formation,especially the grain-filling process,under HLSC mode.A three-year field experiment with four nitrogen rates and three irrigation rates of HLSC was conducted to reveal the response of grain-filling parameters,grain weight percentage of spike weight(GPS),spike moisture content(SMC),and winter wheat yield to water and nitrogen rates.The four nitrogen rates were N1(360 kg ha^(-1) pure N),N2(300 kg ha^(-1) pure N),N3(240 kg ha^(-1) pure N),and N4(180 kg ha^(-1) pure N),respectively,and the three irrigation quotas were W1(120 mm),W2(90 mm),and W3(60 mm),respectively.Results showed that the determinate growth function generally performed well in simulating the temporal dynamics of grain weight(0.989<R^(2)<0.999,where R2 is the determination coefficient).The occurrence time of maximum filling rate(T_(max))and active grain-filling period(AGP)increased with the increase in the water or nitrogen rate,whereas the average grain-filling rate(G_(mean))had a decreasing trend.The final 1,000-grain weight(FTGW)increased and then decreased with the increase in the nitrogen rates and increased with the increase in the irrigation rates.The GPS and SMC had a highly significant quadratic polynomial relationship with grain weight and days after anthesis.Nitrogen,irrigation,and year significantly affected the T_(max),AGP,G_(mean),and FTGW.Particularly,the AGP and FTGW were insignificantly different between high seedbed(HLSC-H)and low seedbed(HLSC-L)across the water and nitrogen levels.Moreover,the moderate water and nitrogen supply was more beneficial for grain yield,as well as for spike number and grain number per hectare.The principal component analysis indicated that combining 240-300 kg N ha^(-1) and 90^(-1)20 mm irrigation quota could improve grain-filling efficiency and yield for the HLSC-cultivated winter wheat.
基金funded by the earmarked fund for China Agriculture Research System(CARS-03-19)the National Natural Science Foundation of China(51879267 and 51709264)+1 种基金the Open Fund Projects of the Agricultural Environment Experimental Station of Minstry of Agriculture and Rural Affairs,China(FIRI2021040103)the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences。
文摘Excessive application of nitrogen (N) fertilizer is the main cause of N loss and poor use efficiency in winter wheat (Triticum aestivum L.) production in the North China Plain (NCP).Drip fertigation is considered to be an effective method for improving N use efficiency and reducing losses,while the performance of drip fertigation in winter wheat is limited by poor N scheduling.A two-year field experiment was conducted to evaluate the growth,development and yield of drip-fertigated winter wheat under different split urea (46%N,240 kg ha^(-1)) applications.The six treatments consisted of five fertigation N application scheduling programs and one slow-release fertilizer (SRF) application.The five N scheduling treatments were N0–100 (0%at sowing and 100%at jointing/booting),N25–75 (25%at sowing and 75%at jointing and booting),N50–50(50%at sowing and 50%at jointing/booting),N75–25 (75%at sowing and 25 at jointing/booting),and N100–0 (100%at sowing and 0%at jointing/booting).The SRF (43%N,240 kg ha^(-1)) was only used as fertilizer at sowing.Split N application significantly (P<0.05) affected wheat grain yield,yield components,aboveground biomass (ABM),water use efficiency(WUE) and nitrogen partial factor productivity (NPFP).The N50–50 and SRF treatments respectively had the highest yield(8.84 and 8.85 t ha^(-1)),ABM (20.67 and 20.83 t ha^(-1)),WUE (2.28 and 2.17 kg m^(-3)) and NPFP (36.82 and 36.88 kg kg^(-1)).This work provided substantial evidence that urea-N applied in equal splits between basal and topdressing doses compete economically with the highly expensive SRF for fertilization of winter wheat crops.Although the single-dose SRF could reduce labor costs involved with the traditional method of manual spreading,the drip fertigation system used in this study with the N50–50 treatment provides an option for farmers to maintain wheat production in the NCP.
基金supported by the National High-Tech R&D Program of China (2013 AA 103005)
文摘Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the minituber production after transplanting. With 100 μmol m^-2s^-1 total photosynthetic photon flux density(PPFD), a light spectrum study of 100% red light emitting diodes(LEDs) light spectrum(RR), 100% blue LEDs light spectrum(BB), 65% red+35% blue LEDs light spectrum(RB), and 45% red+35% blue+20% green LEDs light spectrum(RBG) providing illumination at the in vitro cultured stage of potato plantlets for 4 weeks using fluorescent lamp as control(CK) was performed to investigate the effects of LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro as well as the minituber yield after 2 months transplanting in the greenhouse. Compared to CK, RB and RBG promoted the growth of potato plantlets in vitro with increased stem diameter, plantlet fresh weight, plantlet dry weight, and health index. Furthermore, BB induced the greatest stem diameter as well as the highest health index in potato plantlets in vitro. Root activity, soluble protein, and free amino acid were also significantly enhanced by BB, whereas carbohydrates were improved by RR. In addition, thickness of leaf, palisade parenchyma and spongy parenchyma was significantly increased by BB and RBG. Chloroplasts under BB and RBG showed well-developed grana thylakoid and stroma thylakoid. Unexpectedly, distinct upper epidermis with greatest thickness was induced and palisade parenchyma and spongy parenchyma were arranged neatly in RR. After transplanting in the greenhouse for 2 months, potato plantlets in vitro from BB, RB, and RBG produced high percentage of large size tuber. BB improved fresh and dry weights of the biggest tuber but decreased tuber number per plantlet. In addition, RBG increased tuber number as well as tuber fresh and dry weight slightly. Our results suggested monochromatic blue LEDs as well as combined red, blue or/and green LEDs light spectrum were superior to fluorescent lamp spectrum in micro-propagation of potato plantlets. Therefore, the application of RBG was suitable;BB and RB could be used as alternatives.
基金the National Natural Science Foundation of China(31771793 and 31801358)the National Key Research and Development Program of China(2016YFD0102002)the Natural Science Foundation of Hebei Province(C2019503064)。
文摘Increasing plant density can increase cereal crop yields. However, the physiological and anatomical mechanisms of grain yield increase at high plant densities in maize-based intercropping systems are not well understood. A two-year field experiment was conducted in 2018 and 2019 to investigate grain yield, photosynthetic characteristics, stomatal traits, and leaf anatomy of maize plants in an intercropping system with high plant densities. Two cropping patterns(monocropping and intercropping) and three plant densities(D1, 78,000 plants ha^(-1);D2, 103,500 plants ha^(-1);D3, 129,000 plants ha;were arranged in a randomized block design. Increasing maize plant density significantly increased maize yield, and intercropping gave a significant yield advantage over monocropping under the same plant density. Intercropping combined with high plant density increased the leaf area and SPAD value of maize,increasing the photosynthesis rates after the harvest of pea. At the twelfth leaf stage, the stomatal density and stomatal area of intercrops combined with medium plant density increased by respectively 10.5%and 18.4% relative to their values for the corresponding density of monocrops. Although leaf thickness of maize was reduced by increasing plant density, the chloroplast number and grana lamella number were higher in intercropping than in monocropping under different plant densities. These positive changes in leaf anatomy resulted in increased photosynthesis, suggesting a physiological basis for the increase in grain yield.
基金Supported by Emergency Management Project of Natural Science Foundation of China(61640413)open project of Agricultural Information Research Center in Southern Region of Xinjiang,Agricultural Information Institute of CAAS(ZX2015005)Project of Key Laboratory of Crop Water Use and Regulation,Ministry of Agriculture(FIRI2018-05-03)
文摘With fresh winter jujube from the southern region of Xinjiang as the object of study, the method for detecting pesticide residues on winter jujube surface by near-infrared spectroscopy （NIR） combined with successive projections algorithm （SPA） and partial least squares （PLS） was investigated. The absorbance information of winter jujube sample surface was obtained through NIR technology, for the building of a full-wave band PLS model of fresh winter jujube sample sprayed with different concentrations of pesticide （taking chlorpyrifos as an example） as well as an SPA-PLS model which was built with the characteristic wavelengths extracted with SPA as the input variables for PLS, and the prediction precision of the two kinds of models was compared. The model built with the five characteristic waveslengths extected by SPA method only used the variables 0.32% of all the variables in the full wave band, but its accuracy and precision were better than the model built with the full wave band. It is feasible to build a model for different concentrations of chlorpyrifos on fresh winter jujube surfarce by NIR technology combined with SPA and PLS, and SPA method could simplify the complexity of the model and improve the precision and stablity of the model.
基金funded by the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(FIRI2016-07)
文摘Greenhousing is a technique to bridge season gap in vegetable production and has been widely used worldwide. Calculation of water requirement of crops grown in greenhouse and determination of their irrigation schedules in arid and semi-arid regions are essential for greenhouse maintenance and have thus attracted increased attention over the past decades. The most common method used in the literature to estimate crop evapotranspiration（ET） is the Penman-Monteith（PM） formula. When applied to greenhouse, however, it often uses canopy resistance instead of surface resistance. It is understood that the surface resistance in greenhouse is the result of a combined effect of canopy restriction and soil-surface restriction to water vapor flow, and the relative dominance of one restriction over another depends on crop canopy. In this paper, we developed a surface resistance model in a way similar to two parallel resistances in an electrical circuit to account for both restrictions. Also, considering that wind speed in greenhouse is normally rather small, we compared three methods available in the literature to calculate the aerodynamic resistance, which are the r_a^1 method proposed by Perrier（1975a, b）, the r_a^2 method proposed by Thom and Oliver（1977）, and the r_a^3 method proposed by Zhang and Lemeu（1992）. We validated the model against ET of tomatoes in a greenhouse measured from sap flow system combined with micro-lysimeter in 2015 and with weighing lysimeter in 2016. The results showed that the proposed surface resistance model improved the accuracy of the PM model, especially when the leaf area index was low and the greenhouse was being irrigated. We also found that the aerodynamic resistance calculated from the r_a^1 and r_a^3 methods is applicable to the greenhouse although the latter is slightly more accurate than the former. The proposed surface resistance model, together with the r_a^3 method for aerodynamic resistance, offers an improved approach to estimate ET in greenhouse using the PM formula.
基金Supported by Emergency Management Project of National Natural Science Foundation of China(61640413)Open Project of South Xinjiang Agricultural Information Research Center of Agricultural Information Institute,CAAS(ZX2015005)Key Laboratory Project of Crop Water Use and Regulation,Ministry of Agriculture(FIRI2018-05-03)
文摘[Objective] The paper was to explore a method for detecting chlorpyrifos residues in red Fuji apple. [Method] The original spectral data of apple samples sprayed with different volume fractions of chlorpyrifos were collected using near infrared spectrometer at the band of 4 000-10 000 cm^（-1）. The original spectra were pre-treated by a variety of methods, and partial least squares（PLS） model was established for predictive analysis. [Result] Near infrared spectrum showed sensitivity to apple samples sprayed with different volume fractions of chlorpyrifos, but had low precision on pesticide-free samples. Data of blank control group were further eliminated for modeling prediction. The results showed that the results were the best when pre-treated with first derivative（FD）： R=0.987 9; the square error of prediction（SEP） was 0.173 6; the root-mean-square error of cross-validation（RMSECV） was 0.120 5; and the precision was 0.923 4. [Conclusion] Near infrared spectrum can better predict chlorpyrifos residue, providing a new method for detecting chlorpyrifos residues in Akesu red Fuji apple.
