Objective To analyze the rate of erythrocyte iron incorporation and provided guidance for the iron nutrition for prepubertal children.Methods Fifty-seven prepubertal children of Beijing were involved in this study and...Objective To analyze the rate of erythrocyte iron incorporation and provided guidance for the iron nutrition for prepubertal children.Methods Fifty-seven prepubertal children of Beijing were involved in this study and each subject was orally administered 3 mg of 57 Fe twice daily to obtain a total of 30 mg 57 Fe after a 5-d period. The stable isotope ratios in RBCs were determined in 14 th day, 28 th day, 60 th day, and 90 th day. The erythrocyte incorporation rate in children was calculated using the stable isotope ratios, blood volume and body iron mass.Results The percentage of erythrocyte 57 Fe incorporation increased starting 14 th day, reached a peak at 60 d(boys: 19.67% ± 0.56%, girls: 21.33% ± 0.59%) and then decreased. The erythrocyte incorporation rates of 57 Fe obtained for girls in 60 th day was significantly higher than those obtained for boys(P < 0.0001).Conclusions The oral administration of 57 Fe to children can be used to obtain erythrocyte iron incorporation within 90 d. Prepubertal girls should begin to increase the intake of iron and further studies should pay more attention to the iron status in prepubertal children.展开更多
Objective This study is to obtain precise data on iron physiological requirements in Chinese children using single stable isotope tracertechnique.Methods Thirty boys(10.6±0.2 years)and 27 girls(10.4±0.2 year...Objective This study is to obtain precise data on iron physiological requirements in Chinese children using single stable isotope tracertechnique.Methods Thirty boys(10.6±0.2 years)and 27 girls(10.4±0.2 years)were received oral 6 mg ^(57)Fe each day for 5 consecutive days.Venous blood samples were subsequently drawn to examine the change of total iron concentration and ^(57)Fe abundance at day 0,14,28,60,90,180,360,450,540,630,720.The iron physiological requirement was calculated by iron loss combined with iron circulation rate once ^(57)Fe abundance stabilized in human body.Results The iron physiological requirement was significantly lower in boys than those values in girls(16.88±7.12 vs.18.40±8.81μg/kg per day,P<0.05).Correspondingly,the values were calculated as 722.46±8.43μg/day for boys and 708.40±7.55μg/day for girls,respectively.Considering nearly 10%iron absorption rate,the estimated average iron physiological requirement was 6.0 mg/day in boys and 6.2 mg/day in girls.Conclusion This study indicate that iron physiological requirement could require more daily iron intake in girls as compare with the values in boys having the same body weight.These findings would be facilitate to the new revised dietary reference intakes.展开更多
Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 y...Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 years with body mass index 22.0±1.4 kg/m 2 were recruited. TEE was measured by the DLW method, and basal energy expenditure (BEE) was determined by indirect calorimetry. We also conducted 24-h activity, energy balance and factorial approach to estimate energy requirements of the subjects. Results TEE of subjects by DLW method was 9.45±0.57 MJ/day (2258±180 kcal/day). The 24-h activity was 10.80±0.33 MJ/day (2582±136 kcal/day). The energy requirement, derived from energy balance observations, was 9.93±1.32 MJ/day (2373±315 kcal/day). The BEE of 6.65±0.28 MJ/day (1589±67 kcal/day), calculated by the adjusted Schofield equation, was significantly higher (P<0.001) than that measured by indirect calorimetry, 5.99±0.66 MJ/day (1433±158 kcal/day). The TEE derived from the factorial approach was 10.31±0.43 MJ/day (2463±104 kcal/day). Conclusion The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake (RNI), suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement.展开更多
Objectives To evaluate the distribution by age and sex of serum high-sensitivity C-reactive protein(hsCRP)in an urban Chinese population and to provide a profile prediction for the risk of bacterial infection,inflamma...Objectives To evaluate the distribution by age and sex of serum high-sensitivity C-reactive protein(hsCRP)in an urban Chinese population and to provide a profile prediction for the risk of bacterial infection,inflammatory diseases,or tissue damages in the body.Methods Serum hsCRP was determined using the Roche Tina-quant immuno-turbidimetric assay on a Hitachi 7600–010 automatic biochemical analyzer(Roche Diagnostics)in 1,572 males and 1,800 females,including 78 pregnant women,who were derived from the National Health and Nutrition Survey in2010–2012.Results The average hsCRP concentration in urban China was 0.68 mg/L for males and 0.65 mg/L for females.Significant differences in hsCRP were found among different age groups(P<0.05).Monitoring results showed no significant differences among the 6–11,45–59,and≥60-year-old groups in the comparison of hsCRP between males and females in large cities.However,hsCRP concentration was significantly higher in men aged 12–17 and 18–44 years than in women.Conclusion The distribution of the hsCRP status of residents in large cities in China was influenced by age and gender,and the hsCRP levels of both sexes increased gradually with age.In addition,hsCRP concentration was higher in healthy pregnant women than in non-pregnant women.Basing on our results,we recommend that this parameter be included in future national and international screening for early detection of various illnesses.展开更多
Objective To accurately calculate the protein requirements in Chinese young adults using the indicator amino acid oxidation technique. Methods Nine women and ten men received a restricted daily level of protein intake...Objective To accurately calculate the protein requirements in Chinese young adults using the indicator amino acid oxidation technique. Methods Nine women and ten men received a restricted daily level of protein intake (0.75, 0.82, 0.89, 0.97, and 1.05 g/kg), along with L-[1-13C]-leucine. Subjects' protein requirement was determined by a biphasic linear regression crossover analysis of F13CO2 data. In doing so, a breakpoint at the minimal rate of appearance of 13CO2 expiration specific to each level of dietary protein was identified. This trial was registered with the Chinese clinical trial registry as ChiCTR-ONC-11001407. Results The Estimated Average Requirement (EAR) and the Recommended Nutrient Intake (RNI) of protein for healthy Chinese young adults were determined to be 0.87 and 0.98 g/(kg·d), respectively, based on the indicator amino acid oxidation technique. Conclusion The EAR and RNI of mixed protein are 5% and 16% that are lower than the current proposed EAR and RNI (0.92 and 1.16 g/(kg·d), respectively), as determined by the nitrogen balance method. The respective EAR and RNI recommendations of 0.87 and 0.98 g/(kg·d) of mixed protein are estimated to be reasonable and suitable for Chinese youngadults.展开更多
Iron deficiency anemia is one of the most prevalent nutritional deficiency worldwide. The commonly used cut-off values for identifying iron deficiency are extrapolated from older children and may not be suitable for i...Iron deficiency anemia is one of the most prevalent nutritional deficiency worldwide. The commonly used cut-off values for identifying iron deficiency are extrapolated from older children and may not be suitable for infants. Therefore, our study aimed to establish appropriate cut-off values for the evaluation of iron status in Chinese infants. Pregnant women who delivered at ≥37 gestational weeks with normal iron status were recruited. Later, infants with normal birth weight and who were breastfed in the first 4 months were selected. Blood samples were collected to assess hemoglobin, serum ferritin, soluble transferrin receptor, mean corpuscular volume and free erythrocyte protoporphyrin. Cut-offs of all iron indices were determined as the limit of 95% confidence interval.展开更多
基金supported by the National Natural Science Foundation of China grant number 81330066。
文摘Objective To analyze the rate of erythrocyte iron incorporation and provided guidance for the iron nutrition for prepubertal children.Methods Fifty-seven prepubertal children of Beijing were involved in this study and each subject was orally administered 3 mg of 57 Fe twice daily to obtain a total of 30 mg 57 Fe after a 5-d period. The stable isotope ratios in RBCs were determined in 14 th day, 28 th day, 60 th day, and 90 th day. The erythrocyte incorporation rate in children was calculated using the stable isotope ratios, blood volume and body iron mass.Results The percentage of erythrocyte 57 Fe incorporation increased starting 14 th day, reached a peak at 60 d(boys: 19.67% ± 0.56%, girls: 21.33% ± 0.59%) and then decreased. The erythrocyte incorporation rates of 57 Fe obtained for girls in 60 th day was significantly higher than those obtained for boys(P < 0.0001).Conclusions The oral administration of 57 Fe to children can be used to obtain erythrocyte iron incorporation within 90 d. Prepubertal girls should begin to increase the intake of iron and further studies should pay more attention to the iron status in prepubertal children.
基金supported by the National Natural Science Foundation of China [grant number 81330066]
文摘Objective This study is to obtain precise data on iron physiological requirements in Chinese children using single stable isotope tracertechnique.Methods Thirty boys(10.6±0.2 years)and 27 girls(10.4±0.2 years)were received oral 6 mg ^(57)Fe each day for 5 consecutive days.Venous blood samples were subsequently drawn to examine the change of total iron concentration and ^(57)Fe abundance at day 0,14,28,60,90,180,360,450,540,630,720.The iron physiological requirement was calculated by iron loss combined with iron circulation rate once ^(57)Fe abundance stabilized in human body.Results The iron physiological requirement was significantly lower in boys than those values in girls(16.88±7.12 vs.18.40±8.81μg/kg per day,P<0.05).Correspondingly,the values were calculated as 722.46±8.43μg/day for boys and 708.40±7.55μg/day for girls,respectively.Considering nearly 10%iron absorption rate,the estimated average iron physiological requirement was 6.0 mg/day in boys and 6.2 mg/day in girls.Conclusion This study indicate that iron physiological requirement could require more daily iron intake in girls as compare with the values in boys having the same body weight.These findings would be facilitate to the new revised dietary reference intakes.
基金supported by the projects of National Natural Science Foundation of China (No.30771811)the National Technology Research and Development Program in the Eleventh Five-Year Plan of China(No.2008BAI58B01)
文摘Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 years with body mass index 22.0±1.4 kg/m 2 were recruited. TEE was measured by the DLW method, and basal energy expenditure (BEE) was determined by indirect calorimetry. We also conducted 24-h activity, energy balance and factorial approach to estimate energy requirements of the subjects. Results TEE of subjects by DLW method was 9.45±0.57 MJ/day (2258±180 kcal/day). The 24-h activity was 10.80±0.33 MJ/day (2582±136 kcal/day). The energy requirement, derived from energy balance observations, was 9.93±1.32 MJ/day (2373±315 kcal/day). The BEE of 6.65±0.28 MJ/day (1589±67 kcal/day), calculated by the adjusted Schofield equation, was significantly higher (P<0.001) than that measured by indirect calorimetry, 5.99±0.66 MJ/day (1433±158 kcal/day). The TEE derived from the factorial approach was 10.31±0.43 MJ/day (2463±104 kcal/day). Conclusion The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake (RNI), suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement.
基金supported by the National Key Research and Develepment Program of China 2016YFD0400602。
文摘Objectives To evaluate the distribution by age and sex of serum high-sensitivity C-reactive protein(hsCRP)in an urban Chinese population and to provide a profile prediction for the risk of bacterial infection,inflammatory diseases,or tissue damages in the body.Methods Serum hsCRP was determined using the Roche Tina-quant immuno-turbidimetric assay on a Hitachi 7600–010 automatic biochemical analyzer(Roche Diagnostics)in 1,572 males and 1,800 females,including 78 pregnant women,who were derived from the National Health and Nutrition Survey in2010–2012.Results The average hsCRP concentration in urban China was 0.68 mg/L for males and 0.65 mg/L for females.Significant differences in hsCRP were found among different age groups(P<0.05).Monitoring results showed no significant differences among the 6–11,45–59,and≥60-year-old groups in the comparison of hsCRP between males and females in large cities.However,hsCRP concentration was significantly higher in men aged 12–17 and 18–44 years than in women.Conclusion The distribution of the hsCRP status of residents in large cities in China was influenced by age and gender,and the hsCRP levels of both sexes increased gradually with age.In addition,hsCRP concentration was higher in healthy pregnant women than in non-pregnant women.Basing on our results,we recommend that this parameter be included in future national and international screening for early detection of various illnesses.
基金supported by the National Natural Science Foundation of China (No.81001247): Dietary reference intakes of protein for Chinese youths
文摘Objective To accurately calculate the protein requirements in Chinese young adults using the indicator amino acid oxidation technique. Methods Nine women and ten men received a restricted daily level of protein intake (0.75, 0.82, 0.89, 0.97, and 1.05 g/kg), along with L-[1-13C]-leucine. Subjects' protein requirement was determined by a biphasic linear regression crossover analysis of F13CO2 data. In doing so, a breakpoint at the minimal rate of appearance of 13CO2 expiration specific to each level of dietary protein was identified. This trial was registered with the Chinese clinical trial registry as ChiCTR-ONC-11001407. Results The Estimated Average Requirement (EAR) and the Recommended Nutrient Intake (RNI) of protein for healthy Chinese young adults were determined to be 0.87 and 0.98 g/(kg·d), respectively, based on the indicator amino acid oxidation technique. Conclusion The EAR and RNI of mixed protein are 5% and 16% that are lower than the current proposed EAR and RNI (0.92 and 1.16 g/(kg·d), respectively), as determined by the nitrogen balance method. The respective EAR and RNI recommendations of 0.87 and 0.98 g/(kg·d) of mixed protein are estimated to be reasonable and suitable for Chinese youngadults.
基金supported by Natural Science Foundation of China(Grant No.30972483)The Chinese clinical trial registry number is ChiCTR-TRC-12002838
文摘Iron deficiency anemia is one of the most prevalent nutritional deficiency worldwide. The commonly used cut-off values for identifying iron deficiency are extrapolated from older children and may not be suitable for infants. Therefore, our study aimed to establish appropriate cut-off values for the evaluation of iron status in Chinese infants. Pregnant women who delivered at ≥37 gestational weeks with normal iron status were recruited. Later, infants with normal birth weight and who were breastfed in the first 4 months were selected. Blood samples were collected to assess hemoglobin, serum ferritin, soluble transferrin receptor, mean corpuscular volume and free erythrocyte protoporphyrin. Cut-offs of all iron indices were determined as the limit of 95% confidence interval.
