Toxicity is the totality of adverse effects, which can be functional and morphological lesions in a living organism, caused by a substance introduced in relatively high single doses or in small, repeated doses. The ai...Toxicity is the totality of adverse effects, which can be functional and morphological lesions in a living organism, caused by a substance introduced in relatively high single doses or in small, repeated doses. The aim of this study was to assess the OECD-recommended acute oral toxicity and anti-inflammatory activity of ethanolic extract of Ficus umbellata leaves. Animals were given a single oral dose of 1000, 3000 and 5000 mg/Kg body weight (BW) of the extract. For the anti-inflammatory activity test, rats were given the ethanolic extract of F. umbellata leaves at doses of 100, 300 and 500 mg/Kg or aspirin® at a concentration of 100 mg/Kg PC orally, one hour before injection of 0.05 ml of 1% formalin under the plantar fascia of the rat’s right hind paw. Paw volume measurements were taken one, two and three hours after formalin injection, using an electronic caliper. After 14 days of observation, no deaths were observed in treated rats. The LD50 of ethanolic extract of Ficus umbellata leaf powder is greater than 5000 mg/Kg body weight. This extract has no significant effects on hematological parameters and on the main markers of nephrotoxicity and hepatotoxicity for a single dose of less than 5000 mg/Kg PC. It reduces formalin-induced edema. Evaluation of the percentage inhibition showed that the extract had greater anti-inflammatory activity at 3 hours after the start of the experiment. However, better inhibition of inflammatory oedema of the paw of rats treated with 500 mg/Kg was observed at 5 hours after the start of the experiment, with a percentage inhibition of 69.23 ± 1.02, compared with the reference group treated with aspirin® 100 mg/Kg, which showed an inhibition of 63.50 ± 0.98. These results show that F. umbellata leaves possess anti-inflammatory activity, which would justify their use in traditional African medicine to prevent or treat inflammation.展开更多
One of the most devastating diseases of rice worldwide is bacterial blight (BLB) caused by Xanthomonas oryzae pv. Oryzae (Xoo). In Benin, Xoo was first described in 2013 on wild rice Oryzae longistaminata. So far, no ...One of the most devastating diseases of rice worldwide is bacterial blight (BLB) caused by Xanthomonas oryzae pv. Oryzae (Xoo). In Benin, Xoo was first described in 2013 on wild rice Oryzae longistaminata. So far, no study has been done on Beninese Xoo strains. We do not know whether the pathogen has already passed into the rice varieties grown, or if they are exposed to other bacteria. Whereas the use of resistant varieties, carrying resistance genes, is the only highly effective and environmentally friendly way to control this disease, no information is available on these Xoo resistance genes in rice varieties grown in Benin apart from the one we recently. This study aims to identify Beninese Xoo strains, causing BLB and screen rice varieties grown in Benin for the main resistance genes. Diseased rice leaves showing typical symptoms of fire blight collected from different rice fields in the three phytogeographic areas of Benin were analyzed by PCR for Xoo-specific sequence identification. Furthermore, seventy-five collected rice accessions were screened to identify xa5, Xa7, xa13, and Xa21 resistance genes to Xoo. The results reveal that Xanthomonas oryzae was identified in two fields in Banikouara and one in Malanville. On the other hand, Sphingomonas sp. has been identified in several other rice fields in Benin. Forty-seven of seventy-five rice accessions examined (62.66%) carried Xoo resistance genes with 3 (4%) and 40 (53.33%) of xa5 and Xa21 respectively. None of the accessions had either Xa7 or xa13 resistance genes. Three accessions possess both xa5 and Xa21 genes. Isogenic lines IRBB60 and IRBB21, supposed to be a positive control, presented a Xoo sensitivity allele. These results indicate that Xoo has moved from the wild rice variety to the cultivated variety in northern Benin and varietal improvement programs must be implemented with varieties having several resistance genes for the efficient response against a possible BLB pandemic in Benin.展开更多
The World Health Organization states that foodborne diseases are a worldwide public health issue. Although street foods can provide nutritious and affordable ready-to-eat meals for city dwellers, their health risks ca...The World Health Organization states that foodborne diseases are a worldwide public health issue. Although street foods can provide nutritious and affordable ready-to-eat meals for city dwellers, their health risks can outweigh the benefits. A cross-sectional study was conducted in the Bamako district, focusing on street food vendors near schools, universities, extensive markets, administrative centers, and major roads. We aimed to sample fifty (50) sellers per municipality, making 300 sellers for the Bamako district. We developed a survey sheet to collect data, and six teams rotated between the municipalities each month. Before starting the collection, the teams were provided administrative papers approved by the municipal authority. The survey revealed three types of sales sites: fixed (65%), semi-fixed (30%), and mobile (4.40%). The proportion of sellers was 26.8%, 23.2%, 19.7%, and 4.2% in municipalities III, IV, and I. In municipalities I, II, III, IV, and VI, respectively, 92%, 95.70%, 93%, 87.2%, and 100% of the sellers were female. The age distribution of sellers was 65.63%, 46.81%, 40.82%, 38.30%, 36.17%, 36%, and 32% in the 25-34 and 35 - 44 age groups. Illiteracy rates were 59.20%, 61.70%, 55.30%, 75%, and 56% in municipalities I, II, III, IV, and VI, respectively. The study identified two categories of sellers: 48.3% in type 1 and 51.7% in type 2. The first category comprised 154 sellers, and the second 165 sellers. The survey found that 66.00%, 56.00%, 48.90%, 44.90%, 38.30%, and 34.40% of municipal V, VI, III, I, II, and IV sales sites were open-air. In municipality I, 63.30% of the sites were under hangars, while in municipalities II and IV, the corresponding percentages were 51.10% and 59.40%, respectively. Moreover, 46.00%, 31.90%, 31.30%, 30.60%, and 27.70% of the sites in municipalities VI, II, IV, I, and III were located next to gutters. In conclusion, this study identified several factors that could compromise the quality of street foods sold in the six municipalities of Bamako.展开更多
Spices and aromatic plants are products of plant origin used in food. They are used for the preparation of remedies, for seasoning dishes or for preserving food. This review takes stock of the diversity of spices and ...Spices and aromatic plants are products of plant origin used in food. They are used for the preparation of remedies, for seasoning dishes or for preserving food. This review takes stock of the diversity of spices and aromatic herbs, the chemical composition, the different properties and forms of use of six spices and aromatic herbs commonly used in Benin and around the world. These are Zingiber officinalis (ginger), Curcuma longa (curcuma), Syzygium aromaticum (clove) and three aromatic herbs Petroselinum crispum (parsley), Rosmarinus officinalis (rosemary), and Laurus nobilis (laurel). The methodology used is that of documentary research oriented towards the consultation of previous scientific documents that have highlighted the different pharmacological activities of the different species of spices and aromatic plants targeted. It is important to note that more than twenty plant species are used as spices and aromatic plants in Benin and around the world. Chemically, these different spices and aromatic herbs contain certain secondary metabolites such as flavonoids, tannins, coumarins, alkaloids, steroids, terpenes, saponins, and polyphenols. This diversity of secondary metabolites alone or in a possible synergy may be responsible for many beneficial properties attributed to spices and aromatic herbs.展开更多
One of plant-based products for dental care is plant-based proteolytic enzymes which are principally proteases. In order not to damage the protein and bioactive content, an efficient method should be employed for thei...One of plant-based products for dental care is plant-based proteolytic enzymes which are principally proteases. In order not to damage the protein and bioactive content, an efficient method should be employed for their purifications. As such, three-phase partitioning (TPP) was used to purify protease from moringa (Moringa oleifera). TPP is an emerging, promising, non-chromatographic and economical technology which is simple, quick, efficient and often one-step process for the separation and purification of bioactive molecules from natural sources. It involves the addition of salt (ammonium sulphate) to the crude extract followed by the addition of an organic solvent (butanol). The protein appears as an interfacial precipitate between upper organic solvent and lower aqueous phases. The various conditions such as ammonium sulphate, ratio of crude extract to t-butanol and pH which are required for attaining efficient purification of the protease fractions were optimized. Under optimized conditions, it was seen that, 35% of ammonium sulphate saturation with 1:0.75 ratio of crude extract to t-butanol at pH 7 gave 4.94-fold purification with 96.20% activity yield of protease in the middle phase of the TPP system. The purified enzyme from Moringa oleifera has no antimicrobial effect on the pathogenic bacteria tested. However, this purified enzyme, can be considered as a promising agent, cheap, and safe source which is suitable for using in various industries.展开更多
Background: One of the key drivers for the emergence and spread of antimicrobial resistance (AMR) is non-prudent antibiotic use, which results in selection pressure toward relevant bacteria. Pharmacy staffs have pivot...Background: One of the key drivers for the emergence and spread of antimicrobial resistance (AMR) is non-prudent antibiotic use, which results in selection pressure toward relevant bacteria. Pharmacy staffs have pivotal roles in facilitating the prudent use of antibiotics through antimicrobial stewardship programs. Due to limited information in Benin, this study assessed the knowledge and attitudes of pharmacy staffs on antibiotic use and resistance. Materials and Methods: This cross-sectional study was conducted among 159 pharmacy staffs using a structured questionnaire from August 2018 to December 2018 in Benin. Data analysis was done by using Stata version 13.0. Results: Of the 159 participants, 54.8% knew the definition of antibiotic therapy and 66.9% knew the definition of probabilistic antibiotic therapy. The majority (88.1%) of the participants thought that the choice of antibiotics was made according to the pathology, while 60.4% thought that it depended on the pathology and the germ involved. 49.02% of dispensers in pharmacies stated that half of the patients had requested treatment without providing a prescription at the pharmacy. The top three antibiotics that were dispensed without a prescription include amoxicillin (98.1%), cloxacillin (76.7%) and metronidazole (57.2%), all from the Access group of antibiotics. Conclusion: These results underscore the need for educational and training interventions targeting specific professional groups. There is an urgent need for regulatory measures and public awareness through improved antimicrobial stewardship to limit this practice.展开更多
Introduction: Moringa oleifera was a medicinal plant generally used by populations in the food and therapeutic fields. It’s used to treat anemia has been observed in the Djougou Zone in northern Benin. To our knowled...Introduction: Moringa oleifera was a medicinal plant generally used by populations in the food and therapeutic fields. It’s used to treat anemia has been observed in the Djougou Zone in northern Benin. To our knowledge, there were no scientific data available that have evaluated its efficacy in the treatment of haemolytic anemia. This was what justifies this research work in which the phytochemical analysis, extraction and evaluation of the anti-anemic effect were carried out. Methods: Five groups of five Wistar rats each were formed. All the rats were rendered anemic by injection of phenylhydrazine hydrochloride on the first two days D0 and D1 except those in the negative control group. From the second day, the anemic groups were force-fed either with the aqueous extract of Moringa oleifera leaves at 200 or 300 mg/kg body weight/day, or with vitafer, the reference drug against anemia. The positive control group (anemia) was not treated. Blood samples were taken from all the rats on different days: D0, D2, D7, D10 and D15 to evaluate the data of the hemogram and the osmotic resistance of the red blood cells. Results: Phytochemical analysis revealed the presence of tannins, flavonoids, leucoanthocyanins, saponosides, triterpenes and mucilages. A good yield was obtained at the extraction. Both the extract and the reference drug vitafer completely corrected anemia within two weeks after stimulating hemoglobin synthesis and early release of immature red blood cells into the bloodstream. Its effect seemed dose-dependent and specific. Conclusion: Moringa oleifera leaves showed good therapeutic efficacy and can be considered and exploited for transformation into improved traditional medicines (ITM) in the treatment of anemia.展开更多
The application of biostimulants in agriculture represents an environmentally friendly alternative while increasing agricultural production. The aims of the study were to develop solid biostimulants based on five rhiz...The application of biostimulants in agriculture represents an environmentally friendly alternative while increasing agricultural production. The aims of the study were to develop solid biostimulants based on five rhizobacteria native to Benin’s soils and to evaluate their efficacy on the growth and biomass yield of maize under greenhouse conditions on ferrallitic and ferruginous soils. Clay and peat were used as a conservation binder for the preparation of the biostimulants. These binders were used alone or combined in the different formulations with maize flour and sucrose. 10 g of biostimulants were applied at sowing in pots containing five kilograms of sterilised soil. The experimental design was a completely randomised block of 24 treatments with three replicates. The results obtained showed significant improvements (<em>P</em> < 0.001) in height (49.49%), stem diameter (32.7%), leaf area (66.10%), above-ground biomass (97.12%) and below-ground biomass (53.98%) on ferrallitic soil with the application of the clay + <em>Pseudomonas putida</em> biostimulant compared to the control. On the other hand, the use of the peat biostimulant + <em>Pseudomonas syringae</em> was more beneficial for plant growth on ferruginous soil. The height, stem diameter, leaf area, above-ground biomass and below-ground biomass of the plants under the influence of this biostimulant were improved by 83.06%, 44.57%, 102.94%, 86.84% and 42.68%, respectively, compared to the control. Therefore, these results confirm that Rhizobacteria express their potential through biostimulants formulated on maize. The formulated biostimulants can later be used by producers to improve crop productivity for sustainable agriculture.展开更多
<i><span style="font-family:Verdana;">Annona</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">muricata</span&...<i><span style="font-family:Verdana;">Annona</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> L. (Soursop or Graviola) is a naturally occurring plant seen in Southern part of Africa, traditionally used in Benin to treat various diseases. The present study aimed to investigate phytochemical composition and antioxidant, anti-inflammatory activities of </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves extracts. The secondary metabolites of ethanolic and hemi-ethanolic extracts were analysed by HPLC method. The DPPH and FRAP methods were used to evaluate the antioxidant activity. Inhibition of albumin denaturation method was used to evaluate anti-inflammatory activity of the tested extracts of which larval cytotoxicity was st</span><span><span style="font-family:Verdana;">udied. The major identified compounds were gallic acid, chlorogenic acid, cafeic acid, tannic acid, ferrulic acid, Rutin. Ascorbic acid exhibited the highest inhibition percentage (83.33% ± 0.50%) of DPPH radical with the lowest IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> (45.1 ± 0.28 μg/ml). The inhibition of the ferric ion Fe</span><sup><span style="font-family:Verdana;">3+</span></sup><span style="font-family:Verdana;"> varied (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.0013) according to the extracts type. IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> values of ferric ion inhibition range from 119.5 ± 3.10 to 250.8 ± 2.13 μg/ml respectively for </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves ethanol and hemi-ethanolic extracts. The hemi-ethanolic extract exhibited the highest anti-inflammatory activity (96.66% </span></span><span style="font-family:Verdana;">± 1.17%). The presence of phenolic compound confers to </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves, through the ethanolic and the hemi-ethanolic extracts, the antioxidant and anti-inflammatory activities.展开更多
The purpose of this study is to quantify the polyphenolic content and evaluated anti-inflammatory and antioxidant activity of four plants extracts of Annona senegalensis, Ipomoea batatas, Terminalia superba and Psidiu...The purpose of this study is to quantify the polyphenolic content and evaluated anti-inflammatory and antioxidant activity of four plants extracts of Annona senegalensis, Ipomoea batatas, Terminalia superba and Psidium guajava. Phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminum trichloride methods, respectively with ethanolic extract. The antioxidant activity was accessed by DPPH and FRAP methods. The results of the analysis of this study indicate that the ethanol extracts of the test plants (A. senegalensis, I. batatas, P. guajava and T. superba) are potential source of natural antioxidant and anti-inflammatory.展开更多
Recent ethnopharmacological data cited <i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> as one of the most widely used ...Recent ethnopharmacological data cited <i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> as one of the most widely used medicinal plants in the management of salmonellosis in Benin. However,</span><span> </span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">data related to its activity on non-typhoidal Salmonella spp are limited. This study aimed to assess the antibacterial activity of </span><i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> on multidrug-resistant </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">. Ethanolic and aqueous extracts of </span><i><span style="font-family:Verdana;">S. siamea</span></i><span style="font-family:Verdana;"> were tested for their antibacterial activity on four multidrug-resistant </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">: </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;"> Typhimurium ATCC 14028 and three </span><i><span style="font-family:Verdana;">Salmonella </span></i><span style="font-family:Verdana;">spp</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">. </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">isolated from animals intended for human consumption in Benin. Well diffusion technique combined with the determination by microdilution of Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) were used for antibacterial testing. From antibacterial testing, inhibition diameters of the extracts</span><i> </i><span style="font-family:Verdana;">ranged from 7 to 11 mm, for the susceptible strains. Colistin (reference antibiotic) was active on all </span><i><span style="font-family:Verdana;">Salmonella spp.</span></i><span style="font-family:Verdana;"> with inhibition diameters between 18 and 19 mm. The MICs ranged from 3.125 to 25 mg/ml while MBCs of the extracts are greater than 100 mg/ml, so none of the extracts have antibacterial power (p.a). From these results, it appears that </span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">the </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">use of </span><i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> in the traditional treatment of salmonellosis is justified. These results must be valued in the development of anti-</span><i><span style="font-family:Verdana;">salmonella</span></i><span style="font-family:Verdana;"> phytomedicines.</span></span></span></span>展开更多
Introduction: Jatropha multifida Lin was a plant of traditional Beninese medicine used as an antibiotic. This study aimed to evaluate the antibacterial activity and the toxicity of the sap and the aqueous extract of J...Introduction: Jatropha multifida Lin was a plant of traditional Beninese medicine used as an antibiotic. This study aimed to evaluate the antibacterial activity and the toxicity of the sap and the aqueous extract of Jatropha multifida leaves. Methods: Phytochemical screening of Jatropha multifida leaves was carried out. The extract was obtained by maceration. The antimicrobial activity of sap and leaves was evaluated on the five strains of hospital germs. Acute oral toxicity by forced gavage in a single dose of 2000 mg/kg body weight was performed on female Wistar rats. Biochemical and hematological parameters were determined. Results: The presence of flavonoids, tannins, alkaloids, anthocyanins, mucilages, leuco-anthocyanins and saponosides was noted in the leaves of Jatropha multifida. The aqueous extracts of the leaves inhibited two strains of Staphylococcus aureus out of three, while the sap of Jatropha multifida was 100% bactericidal against the strains of Staphylococcus aureus and Streptococcus D. The sap and aqueous leaf extract were not bactericidal on strains of Escherichia coli, Klebsiella oxytoca and Pseudomonas aeruginosa. For the toxicity, there was no death of rats and the aqueous extract of the leaves did not significantly vary the weight of the rats, the creatinine, the ALAT transaminase, the hemoglobin level, the number of white blood cells and blood platelets. Conclusion: The sap of Jatropha multifida exerted a more effective antibacterial activity than the aqueous extract of its leaves. The leaves were not acutely toxic.展开更多
Malaria is a parasitic disease caused by the bite of female Anopheles mosquito and particularly affects the tropical areas of the world. According to national statistics it is the leading cause of consultations and ho...Malaria is a parasitic disease caused by the bite of female Anopheles mosquito and particularly affects the tropical areas of the world. According to national statistics it is the leading cause of consultations and hospitalizations. Nowadays, despite the surveillance systems for efficient malaria control and access to generic drugs, Benin is witnessing an increased development of illicit drug markets with a large part of the population going towards such markets. However, this is not without adverse impact on the health of individuals, as well as, the economic status of the country. Therefore, the situation needs to be seriously considered by policy makers at various levels, health professionals but also the entire international community in order to thwart this scourge.Regarding the aforementioned situation, the current study was undertaken aiming to perform a quality control of selected antimalarial drugs of the illegal market in Porto-Novo city. Therefore, 40 antimalarial batches were randomly collected in the illicit drug market and submitted to analytical tests such as: macroscopic examination (a visual and critical examination);mass uniformity test;disintegration test;identification test and active ingredients’ content measurement. At the end of the study, the percentages of non-compliance is 97.5%, 5%, 15% and 27.5%, respectively for the content uniformity tests, disintegration, identification and assay. Over-all, 42.5% of noncompliance was recorded. The findings of this study prove that street vended drugs offer no guarantee of good quality and pose a threat to the health of populations. Also, the rate of non-compliance denotes a flaw in the security of the drug distribution system.展开更多
Healthcare-associated infections (HCAIs) remain a major public health concern. The aim of this study was to characterize potential pathogenic bacteria isolated in high-risk infectious services at the University Hospit...Healthcare-associated infections (HCAIs) remain a major public health concern. The aim of this study was to characterize potential pathogenic bacteria isolated in high-risk infectious services at the University Hospital Center of Suru-Léré in Benin. A cross-sectional, descriptive study was carried out on 215 samples with 8 air samples, 20 hand samples and 187 samples of medical-technical material. Genes for resistance to <i>β</i>-lactam antibiotics (blaTEM, blaSHV, blaCTX-M and blaOXA-1) were sought. The data were processed and analyzed using EPI Info version 3.5.4 software. A total of 10 bacterial species were identified. Of the 319 bacterial strains identified, <i>Staphylococcus saprophyticus</i>, <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i> were predominant at 20.06%, 17.87% and 11.28% respectively. Antibiotic susceptibility showed that the majority of bacterial strains were multidrug-resistant. Of the 4 resistance genes sought, only the blaTEM and blaSHV genes were found. The poor microbiological quality observed in high-risk infectious services could be a risk factor for healthcare-associated infections. It is therefore useful to implement preventive measures in order to prevent or reduce the risk of healthcare-associated infections.展开更多
This study focused on the transfer of experimental results of anaerobic digestion of liquid waste from an attiéké (steamed cassava semolina) factory to a 6 m<sup>3</sup> pilot digester. The exper...This study focused on the transfer of experimental results of anaerobic digestion of liquid waste from an attiéké (steamed cassava semolina) factory to a 6 m<sup>3</sup> pilot digester. The experimental digester and the pilot were powered as follows: Lw + U + C (liquid waste + urine + cow dung). To the results, the experimental digester mesophilic with a progressive elimination of COD. Also, the nitrogen concentrations in the experimental reactor had little removal with alkaline pH. As for the biogas product in this digester, a volume of 3.6 m<sup>3</sup> was obtained with a positive flammability test. The transition from the laboratory scale to the semi-industrial scale retains the results of purification and fuel biogas production of the experimental digester.展开更多
In Benin, the preservation of maize is a real problem involving important physical and chemical properties losses. Physical losses are due by rodents, pests and insects and chemical losses are mainly due to fungal inf...In Benin, the preservation of maize is a real problem involving important physical and chemical properties losses. Physical losses are due by rodents, pests and insects and chemical losses are mainly due to fungal infection. Several methods, products and practices are used by farmers to overcome these problems. The methods used do not always control the losses caused by fungal infection. The objective of the study was to make a bibliographic synthesis of the impacts induced by the items, practices and methods used to overcome the chemical losses of maize. In the search for information, the library of the Ministry of Agriculture, Livestock and Fisheries was consulted;the archives of the library of the University of Abomey-Calavi and the National Institute of Agricultural Research of Benin were also consulted. Thus, chemicals such as sofagrain, Pyrimiphos-Methyl, Thiamethoxam, Pyrimiphos-Methyl, Permethrin, Deltamethrin, fumigant are used to control physical attacks on maize. Ash and plants species such as neem tree (<em>Azadirachta indica</em>) are generally used for the preservation and storage of maize seeds. In addition, chemicals such as arsenious anhydride and zinc phosphide are used for the preservation of corn. The use of these molecules and chemical products has negative impacts on human health and the environment. Of all the methods used, no treatment is still popularized for the reduction of the chemical losses caused by the fungal infection in corn storage.展开更多
Urinary tract infections remain the most common bacterial infection in human population. They are also one of the most frequently occurring nosocomial infections, representing about 40% of all nosocomial bacterial inf...Urinary tract infections remain the most common bacterial infection in human population. They are also one of the most frequently occurring nosocomial infections, representing about 40% of all nosocomial bacterial infections world widely and associated with important medical and financial implications. This retrospective study aimed to establish the bacterial profile of UTIs in Benin. Therefore, reported data were collected from 2003 to 2012 at Menontin’s Hospital in Cotonou and subjected to analyses. At the end of the study, fourteen different bacteria species were found to be associated with UTI cases in Benin. Escherichia coli was the most prevalent followed by Staphylococcus aureus. Results of the current study are helpful to the health care community of Benin in establishing the appropriate antibiotherapy for UTI patients’ treatment with respect organisms into circulation.展开更多
Since the time of our ancestors,natural products issued from plant play a therapeutic crucial role.About 25-30%of all medicines(drugs)available for the treatment of diseases are derived from natural products(from plan...Since the time of our ancestors,natural products issued from plant play a therapeutic crucial role.About 25-30%of all medicines(drugs)available for the treatment of diseases are derived from natural products(from plants,animals,bacteria and fungi)or are derivatives of natural products.The aim of this research was to scientifically identify and supply tangible documentation on these two plants employed in the traditional medicine.From November 2020 to February 2021,an ethnobotanical survey was conducted within four markets at the south of Benin,employing a semi-structured questionnaire.Two hundred respondents including 80%of females and 20%of males were interviewed.The 2 studied plants are mainly used for different types of sickness related to infections.Females’herbalists are the most represented.From this research,it appears that the 2 plants are widely used for the treatment of severe infections.On the market,95%of the leafy steam are sold against 5%of the roots for both plants.The main preparation way is decoction.Oral use is reported to be common in all region.The value of samples sold varies from 200F CFA(Financial Cooperation of Africa)to 1000F CFA.The decoctions are usually obtained through one of a mix of different types of plants.Traditional knowledge is transmitted from one generation to another by oral education.Till today there was no record found.During our study,we did no record prohibition or side effect related to these plants’use.These medicinal plants occupy a crucial place within the therapeutic arsenal of west Africa.Our results constitute a vital tool to determine the true potentials of these plants.These results could lead to new improved traditional medicine.展开更多
Fish is rich </span><span style="font-size:10.0pt;font-family:"">in</span><span style="font-size:10.0pt;font-family:""> essential nutrients but can remain a potent...Fish is rich </span><span style="font-size:10.0pt;font-family:"">in</span><span style="font-size:10.0pt;font-family:""> essential nutrients but can remain a potential source of food poisoning. A study was</span><span style="font-size:10.0pt;font-family:"">,</span><span style="font-size:10.0pt;font-family:""> therefore</span><span style="font-size:10.0pt;font-family:"">,</span><span style="font-size:10.0pt;font-family:""> carried out with the general objective of highlighting the probable biological and chemical dangers associated with the <span>consumption of fish and freshwater fruits from </span></span><span style="font-size:10.0pt;font-family:"">the </span><span style="font-size:10.0pt;font-family:"">Guéssabo river. The</span><span style="font-size:10.0pt;font-family:""> present study was conducted on 50 carp fish, 50 mackerel f</span><span style="font-size:10.0pt;font-family:"">i</span><span style="font-size:10.0pt;font-family:"">sh, 50 frog</span><span style="font-size:10.0pt;font-family:"">s</span><span style="font-size:10.0pt;font-family:""> and 50 mol<span>lusks caught in the Guéssabo River. Microorganisms were enumerated by</span> conventional tests and heavy metals were investigated by Inductively Coupled Plasma Optical Emission Spectrometry. The enumeration showed high levels of thermotolerant Coliforms (2.5 × 10<sup>4</sup>), Enterobacteriaceae (3.4 × 10<sup>4</sup>), <i>S</i>. <i>aureus</i> (2.3 × 10<sup>3</sup>) and Yeast/Molds (2.1 × 10<sup>4</sup>), in all samples the standards were not in conformity with the required standards. High levels of Al (7230 μg/kg) and Cd (21.57 μg/kg) were observed in carp fish and these values are <span>above the standard. In conclusion</span></span><span style="font-size:10.0pt;font-family:"">,</span><span style="font-size:10.0pt;font-family:""> the fish caught in the Guéssabo River</span><span style="font-size:10.0pt;font-family:""> could be a health risk factor for the consumer.展开更多
文摘Toxicity is the totality of adverse effects, which can be functional and morphological lesions in a living organism, caused by a substance introduced in relatively high single doses or in small, repeated doses. The aim of this study was to assess the OECD-recommended acute oral toxicity and anti-inflammatory activity of ethanolic extract of Ficus umbellata leaves. Animals were given a single oral dose of 1000, 3000 and 5000 mg/Kg body weight (BW) of the extract. For the anti-inflammatory activity test, rats were given the ethanolic extract of F. umbellata leaves at doses of 100, 300 and 500 mg/Kg or aspirin® at a concentration of 100 mg/Kg PC orally, one hour before injection of 0.05 ml of 1% formalin under the plantar fascia of the rat’s right hind paw. Paw volume measurements were taken one, two and three hours after formalin injection, using an electronic caliper. After 14 days of observation, no deaths were observed in treated rats. The LD50 of ethanolic extract of Ficus umbellata leaf powder is greater than 5000 mg/Kg body weight. This extract has no significant effects on hematological parameters and on the main markers of nephrotoxicity and hepatotoxicity for a single dose of less than 5000 mg/Kg PC. It reduces formalin-induced edema. Evaluation of the percentage inhibition showed that the extract had greater anti-inflammatory activity at 3 hours after the start of the experiment. However, better inhibition of inflammatory oedema of the paw of rats treated with 500 mg/Kg was observed at 5 hours after the start of the experiment, with a percentage inhibition of 69.23 ± 1.02, compared with the reference group treated with aspirin® 100 mg/Kg, which showed an inhibition of 63.50 ± 0.98. These results show that F. umbellata leaves possess anti-inflammatory activity, which would justify their use in traditional African medicine to prevent or treat inflammation.
文摘One of the most devastating diseases of rice worldwide is bacterial blight (BLB) caused by Xanthomonas oryzae pv. Oryzae (Xoo). In Benin, Xoo was first described in 2013 on wild rice Oryzae longistaminata. So far, no study has been done on Beninese Xoo strains. We do not know whether the pathogen has already passed into the rice varieties grown, or if they are exposed to other bacteria. Whereas the use of resistant varieties, carrying resistance genes, is the only highly effective and environmentally friendly way to control this disease, no information is available on these Xoo resistance genes in rice varieties grown in Benin apart from the one we recently. This study aims to identify Beninese Xoo strains, causing BLB and screen rice varieties grown in Benin for the main resistance genes. Diseased rice leaves showing typical symptoms of fire blight collected from different rice fields in the three phytogeographic areas of Benin were analyzed by PCR for Xoo-specific sequence identification. Furthermore, seventy-five collected rice accessions were screened to identify xa5, Xa7, xa13, and Xa21 resistance genes to Xoo. The results reveal that Xanthomonas oryzae was identified in two fields in Banikouara and one in Malanville. On the other hand, Sphingomonas sp. has been identified in several other rice fields in Benin. Forty-seven of seventy-five rice accessions examined (62.66%) carried Xoo resistance genes with 3 (4%) and 40 (53.33%) of xa5 and Xa21 respectively. None of the accessions had either Xa7 or xa13 resistance genes. Three accessions possess both xa5 and Xa21 genes. Isogenic lines IRBB60 and IRBB21, supposed to be a positive control, presented a Xoo sensitivity allele. These results indicate that Xoo has moved from the wild rice variety to the cultivated variety in northern Benin and varietal improvement programs must be implemented with varieties having several resistance genes for the efficient response against a possible BLB pandemic in Benin.
文摘The World Health Organization states that foodborne diseases are a worldwide public health issue. Although street foods can provide nutritious and affordable ready-to-eat meals for city dwellers, their health risks can outweigh the benefits. A cross-sectional study was conducted in the Bamako district, focusing on street food vendors near schools, universities, extensive markets, administrative centers, and major roads. We aimed to sample fifty (50) sellers per municipality, making 300 sellers for the Bamako district. We developed a survey sheet to collect data, and six teams rotated between the municipalities each month. Before starting the collection, the teams were provided administrative papers approved by the municipal authority. The survey revealed three types of sales sites: fixed (65%), semi-fixed (30%), and mobile (4.40%). The proportion of sellers was 26.8%, 23.2%, 19.7%, and 4.2% in municipalities III, IV, and I. In municipalities I, II, III, IV, and VI, respectively, 92%, 95.70%, 93%, 87.2%, and 100% of the sellers were female. The age distribution of sellers was 65.63%, 46.81%, 40.82%, 38.30%, 36.17%, 36%, and 32% in the 25-34 and 35 - 44 age groups. Illiteracy rates were 59.20%, 61.70%, 55.30%, 75%, and 56% in municipalities I, II, III, IV, and VI, respectively. The study identified two categories of sellers: 48.3% in type 1 and 51.7% in type 2. The first category comprised 154 sellers, and the second 165 sellers. The survey found that 66.00%, 56.00%, 48.90%, 44.90%, 38.30%, and 34.40% of municipal V, VI, III, I, II, and IV sales sites were open-air. In municipality I, 63.30% of the sites were under hangars, while in municipalities II and IV, the corresponding percentages were 51.10% and 59.40%, respectively. Moreover, 46.00%, 31.90%, 31.30%, 30.60%, and 27.70% of the sites in municipalities VI, II, IV, I, and III were located next to gutters. In conclusion, this study identified several factors that could compromise the quality of street foods sold in the six municipalities of Bamako.
文摘Spices and aromatic plants are products of plant origin used in food. They are used for the preparation of remedies, for seasoning dishes or for preserving food. This review takes stock of the diversity of spices and aromatic herbs, the chemical composition, the different properties and forms of use of six spices and aromatic herbs commonly used in Benin and around the world. These are Zingiber officinalis (ginger), Curcuma longa (curcuma), Syzygium aromaticum (clove) and three aromatic herbs Petroselinum crispum (parsley), Rosmarinus officinalis (rosemary), and Laurus nobilis (laurel). The methodology used is that of documentary research oriented towards the consultation of previous scientific documents that have highlighted the different pharmacological activities of the different species of spices and aromatic plants targeted. It is important to note that more than twenty plant species are used as spices and aromatic plants in Benin and around the world. Chemically, these different spices and aromatic herbs contain certain secondary metabolites such as flavonoids, tannins, coumarins, alkaloids, steroids, terpenes, saponins, and polyphenols. This diversity of secondary metabolites alone or in a possible synergy may be responsible for many beneficial properties attributed to spices and aromatic herbs.
文摘One of plant-based products for dental care is plant-based proteolytic enzymes which are principally proteases. In order not to damage the protein and bioactive content, an efficient method should be employed for their purifications. As such, three-phase partitioning (TPP) was used to purify protease from moringa (Moringa oleifera). TPP is an emerging, promising, non-chromatographic and economical technology which is simple, quick, efficient and often one-step process for the separation and purification of bioactive molecules from natural sources. It involves the addition of salt (ammonium sulphate) to the crude extract followed by the addition of an organic solvent (butanol). The protein appears as an interfacial precipitate between upper organic solvent and lower aqueous phases. The various conditions such as ammonium sulphate, ratio of crude extract to t-butanol and pH which are required for attaining efficient purification of the protease fractions were optimized. Under optimized conditions, it was seen that, 35% of ammonium sulphate saturation with 1:0.75 ratio of crude extract to t-butanol at pH 7 gave 4.94-fold purification with 96.20% activity yield of protease in the middle phase of the TPP system. The purified enzyme from Moringa oleifera has no antimicrobial effect on the pathogenic bacteria tested. However, this purified enzyme, can be considered as a promising agent, cheap, and safe source which is suitable for using in various industries.
文摘Background: One of the key drivers for the emergence and spread of antimicrobial resistance (AMR) is non-prudent antibiotic use, which results in selection pressure toward relevant bacteria. Pharmacy staffs have pivotal roles in facilitating the prudent use of antibiotics through antimicrobial stewardship programs. Due to limited information in Benin, this study assessed the knowledge and attitudes of pharmacy staffs on antibiotic use and resistance. Materials and Methods: This cross-sectional study was conducted among 159 pharmacy staffs using a structured questionnaire from August 2018 to December 2018 in Benin. Data analysis was done by using Stata version 13.0. Results: Of the 159 participants, 54.8% knew the definition of antibiotic therapy and 66.9% knew the definition of probabilistic antibiotic therapy. The majority (88.1%) of the participants thought that the choice of antibiotics was made according to the pathology, while 60.4% thought that it depended on the pathology and the germ involved. 49.02% of dispensers in pharmacies stated that half of the patients had requested treatment without providing a prescription at the pharmacy. The top three antibiotics that were dispensed without a prescription include amoxicillin (98.1%), cloxacillin (76.7%) and metronidazole (57.2%), all from the Access group of antibiotics. Conclusion: These results underscore the need for educational and training interventions targeting specific professional groups. There is an urgent need for regulatory measures and public awareness through improved antimicrobial stewardship to limit this practice.
文摘Introduction: Moringa oleifera was a medicinal plant generally used by populations in the food and therapeutic fields. It’s used to treat anemia has been observed in the Djougou Zone in northern Benin. To our knowledge, there were no scientific data available that have evaluated its efficacy in the treatment of haemolytic anemia. This was what justifies this research work in which the phytochemical analysis, extraction and evaluation of the anti-anemic effect were carried out. Methods: Five groups of five Wistar rats each were formed. All the rats were rendered anemic by injection of phenylhydrazine hydrochloride on the first two days D0 and D1 except those in the negative control group. From the second day, the anemic groups were force-fed either with the aqueous extract of Moringa oleifera leaves at 200 or 300 mg/kg body weight/day, or with vitafer, the reference drug against anemia. The positive control group (anemia) was not treated. Blood samples were taken from all the rats on different days: D0, D2, D7, D10 and D15 to evaluate the data of the hemogram and the osmotic resistance of the red blood cells. Results: Phytochemical analysis revealed the presence of tannins, flavonoids, leucoanthocyanins, saponosides, triterpenes and mucilages. A good yield was obtained at the extraction. Both the extract and the reference drug vitafer completely corrected anemia within two weeks after stimulating hemoglobin synthesis and early release of immature red blood cells into the bloodstream. Its effect seemed dose-dependent and specific. Conclusion: Moringa oleifera leaves showed good therapeutic efficacy and can be considered and exploited for transformation into improved traditional medicines (ITM) in the treatment of anemia.
文摘The application of biostimulants in agriculture represents an environmentally friendly alternative while increasing agricultural production. The aims of the study were to develop solid biostimulants based on five rhizobacteria native to Benin’s soils and to evaluate their efficacy on the growth and biomass yield of maize under greenhouse conditions on ferrallitic and ferruginous soils. Clay and peat were used as a conservation binder for the preparation of the biostimulants. These binders were used alone or combined in the different formulations with maize flour and sucrose. 10 g of biostimulants were applied at sowing in pots containing five kilograms of sterilised soil. The experimental design was a completely randomised block of 24 treatments with three replicates. The results obtained showed significant improvements (<em>P</em> < 0.001) in height (49.49%), stem diameter (32.7%), leaf area (66.10%), above-ground biomass (97.12%) and below-ground biomass (53.98%) on ferrallitic soil with the application of the clay + <em>Pseudomonas putida</em> biostimulant compared to the control. On the other hand, the use of the peat biostimulant + <em>Pseudomonas syringae</em> was more beneficial for plant growth on ferruginous soil. The height, stem diameter, leaf area, above-ground biomass and below-ground biomass of the plants under the influence of this biostimulant were improved by 83.06%, 44.57%, 102.94%, 86.84% and 42.68%, respectively, compared to the control. Therefore, these results confirm that Rhizobacteria express their potential through biostimulants formulated on maize. The formulated biostimulants can later be used by producers to improve crop productivity for sustainable agriculture.
文摘<i><span style="font-family:Verdana;">Annona</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> L. (Soursop or Graviola) is a naturally occurring plant seen in Southern part of Africa, traditionally used in Benin to treat various diseases. The present study aimed to investigate phytochemical composition and antioxidant, anti-inflammatory activities of </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves extracts. The secondary metabolites of ethanolic and hemi-ethanolic extracts were analysed by HPLC method. The DPPH and FRAP methods were used to evaluate the antioxidant activity. Inhibition of albumin denaturation method was used to evaluate anti-inflammatory activity of the tested extracts of which larval cytotoxicity was st</span><span><span style="font-family:Verdana;">udied. The major identified compounds were gallic acid, chlorogenic acid, cafeic acid, tannic acid, ferrulic acid, Rutin. Ascorbic acid exhibited the highest inhibition percentage (83.33% ± 0.50%) of DPPH radical with the lowest IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> (45.1 ± 0.28 μg/ml). The inhibition of the ferric ion Fe</span><sup><span style="font-family:Verdana;">3+</span></sup><span style="font-family:Verdana;"> varied (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.0013) according to the extracts type. IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> values of ferric ion inhibition range from 119.5 ± 3.10 to 250.8 ± 2.13 μg/ml respectively for </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves ethanol and hemi-ethanolic extracts. The hemi-ethanolic extract exhibited the highest anti-inflammatory activity (96.66% </span></span><span style="font-family:Verdana;">± 1.17%). The presence of phenolic compound confers to </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">muricata</span></i><span style="font-family:Verdana;"> leaves, through the ethanolic and the hemi-ethanolic extracts, the antioxidant and anti-inflammatory activities.
文摘The purpose of this study is to quantify the polyphenolic content and evaluated anti-inflammatory and antioxidant activity of four plants extracts of Annona senegalensis, Ipomoea batatas, Terminalia superba and Psidium guajava. Phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminum trichloride methods, respectively with ethanolic extract. The antioxidant activity was accessed by DPPH and FRAP methods. The results of the analysis of this study indicate that the ethanol extracts of the test plants (A. senegalensis, I. batatas, P. guajava and T. superba) are potential source of natural antioxidant and anti-inflammatory.
文摘Recent ethnopharmacological data cited <i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> as one of the most widely used medicinal plants in the management of salmonellosis in Benin. However,</span><span> </span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">data related to its activity on non-typhoidal Salmonella spp are limited. This study aimed to assess the antibacterial activity of </span><i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> on multidrug-resistant </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">. Ethanolic and aqueous extracts of </span><i><span style="font-family:Verdana;">S. siamea</span></i><span style="font-family:Verdana;"> were tested for their antibacterial activity on four multidrug-resistant </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">: </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;"> Typhimurium ATCC 14028 and three </span><i><span style="font-family:Verdana;">Salmonella </span></i><span style="font-family:Verdana;">spp</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">. </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">isolated from animals intended for human consumption in Benin. Well diffusion technique combined with the determination by microdilution of Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) were used for antibacterial testing. From antibacterial testing, inhibition diameters of the extracts</span><i> </i><span style="font-family:Verdana;">ranged from 7 to 11 mm, for the susceptible strains. Colistin (reference antibiotic) was active on all </span><i><span style="font-family:Verdana;">Salmonella spp.</span></i><span style="font-family:Verdana;"> with inhibition diameters between 18 and 19 mm. The MICs ranged from 3.125 to 25 mg/ml while MBCs of the extracts are greater than 100 mg/ml, so none of the extracts have antibacterial power (p.a). From these results, it appears that </span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">the </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">use of </span><i><span style="font-family:Verdana;">Senna siamea</span></i><span style="font-family:Verdana;"> in the traditional treatment of salmonellosis is justified. These results must be valued in the development of anti-</span><i><span style="font-family:Verdana;">salmonella</span></i><span style="font-family:Verdana;"> phytomedicines.</span></span></span></span>
文摘Introduction: Jatropha multifida Lin was a plant of traditional Beninese medicine used as an antibiotic. This study aimed to evaluate the antibacterial activity and the toxicity of the sap and the aqueous extract of Jatropha multifida leaves. Methods: Phytochemical screening of Jatropha multifida leaves was carried out. The extract was obtained by maceration. The antimicrobial activity of sap and leaves was evaluated on the five strains of hospital germs. Acute oral toxicity by forced gavage in a single dose of 2000 mg/kg body weight was performed on female Wistar rats. Biochemical and hematological parameters were determined. Results: The presence of flavonoids, tannins, alkaloids, anthocyanins, mucilages, leuco-anthocyanins and saponosides was noted in the leaves of Jatropha multifida. The aqueous extracts of the leaves inhibited two strains of Staphylococcus aureus out of three, while the sap of Jatropha multifida was 100% bactericidal against the strains of Staphylococcus aureus and Streptococcus D. The sap and aqueous leaf extract were not bactericidal on strains of Escherichia coli, Klebsiella oxytoca and Pseudomonas aeruginosa. For the toxicity, there was no death of rats and the aqueous extract of the leaves did not significantly vary the weight of the rats, the creatinine, the ALAT transaminase, the hemoglobin level, the number of white blood cells and blood platelets. Conclusion: The sap of Jatropha multifida exerted a more effective antibacterial activity than the aqueous extract of its leaves. The leaves were not acutely toxic.
文摘Malaria is a parasitic disease caused by the bite of female Anopheles mosquito and particularly affects the tropical areas of the world. According to national statistics it is the leading cause of consultations and hospitalizations. Nowadays, despite the surveillance systems for efficient malaria control and access to generic drugs, Benin is witnessing an increased development of illicit drug markets with a large part of the population going towards such markets. However, this is not without adverse impact on the health of individuals, as well as, the economic status of the country. Therefore, the situation needs to be seriously considered by policy makers at various levels, health professionals but also the entire international community in order to thwart this scourge.Regarding the aforementioned situation, the current study was undertaken aiming to perform a quality control of selected antimalarial drugs of the illegal market in Porto-Novo city. Therefore, 40 antimalarial batches were randomly collected in the illicit drug market and submitted to analytical tests such as: macroscopic examination (a visual and critical examination);mass uniformity test;disintegration test;identification test and active ingredients’ content measurement. At the end of the study, the percentages of non-compliance is 97.5%, 5%, 15% and 27.5%, respectively for the content uniformity tests, disintegration, identification and assay. Over-all, 42.5% of noncompliance was recorded. The findings of this study prove that street vended drugs offer no guarantee of good quality and pose a threat to the health of populations. Also, the rate of non-compliance denotes a flaw in the security of the drug distribution system.
文摘Healthcare-associated infections (HCAIs) remain a major public health concern. The aim of this study was to characterize potential pathogenic bacteria isolated in high-risk infectious services at the University Hospital Center of Suru-Léré in Benin. A cross-sectional, descriptive study was carried out on 215 samples with 8 air samples, 20 hand samples and 187 samples of medical-technical material. Genes for resistance to <i>β</i>-lactam antibiotics (blaTEM, blaSHV, blaCTX-M and blaOXA-1) were sought. The data were processed and analyzed using EPI Info version 3.5.4 software. A total of 10 bacterial species were identified. Of the 319 bacterial strains identified, <i>Staphylococcus saprophyticus</i>, <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i> were predominant at 20.06%, 17.87% and 11.28% respectively. Antibiotic susceptibility showed that the majority of bacterial strains were multidrug-resistant. Of the 4 resistance genes sought, only the blaTEM and blaSHV genes were found. The poor microbiological quality observed in high-risk infectious services could be a risk factor for healthcare-associated infections. It is therefore useful to implement preventive measures in order to prevent or reduce the risk of healthcare-associated infections.
文摘This study focused on the transfer of experimental results of anaerobic digestion of liquid waste from an attiéké (steamed cassava semolina) factory to a 6 m<sup>3</sup> pilot digester. The experimental digester and the pilot were powered as follows: Lw + U + C (liquid waste + urine + cow dung). To the results, the experimental digester mesophilic with a progressive elimination of COD. Also, the nitrogen concentrations in the experimental reactor had little removal with alkaline pH. As for the biogas product in this digester, a volume of 3.6 m<sup>3</sup> was obtained with a positive flammability test. The transition from the laboratory scale to the semi-industrial scale retains the results of purification and fuel biogas production of the experimental digester.
文摘In Benin, the preservation of maize is a real problem involving important physical and chemical properties losses. Physical losses are due by rodents, pests and insects and chemical losses are mainly due to fungal infection. Several methods, products and practices are used by farmers to overcome these problems. The methods used do not always control the losses caused by fungal infection. The objective of the study was to make a bibliographic synthesis of the impacts induced by the items, practices and methods used to overcome the chemical losses of maize. In the search for information, the library of the Ministry of Agriculture, Livestock and Fisheries was consulted;the archives of the library of the University of Abomey-Calavi and the National Institute of Agricultural Research of Benin were also consulted. Thus, chemicals such as sofagrain, Pyrimiphos-Methyl, Thiamethoxam, Pyrimiphos-Methyl, Permethrin, Deltamethrin, fumigant are used to control physical attacks on maize. Ash and plants species such as neem tree (<em>Azadirachta indica</em>) are generally used for the preservation and storage of maize seeds. In addition, chemicals such as arsenious anhydride and zinc phosphide are used for the preservation of corn. The use of these molecules and chemical products has negative impacts on human health and the environment. Of all the methods used, no treatment is still popularized for the reduction of the chemical losses caused by the fungal infection in corn storage.
文摘Urinary tract infections remain the most common bacterial infection in human population. They are also one of the most frequently occurring nosocomial infections, representing about 40% of all nosocomial bacterial infections world widely and associated with important medical and financial implications. This retrospective study aimed to establish the bacterial profile of UTIs in Benin. Therefore, reported data were collected from 2003 to 2012 at Menontin’s Hospital in Cotonou and subjected to analyses. At the end of the study, fourteen different bacteria species were found to be associated with UTI cases in Benin. Escherichia coli was the most prevalent followed by Staphylococcus aureus. Results of the current study are helpful to the health care community of Benin in establishing the appropriate antibiotherapy for UTI patients’ treatment with respect organisms into circulation.
文摘Since the time of our ancestors,natural products issued from plant play a therapeutic crucial role.About 25-30%of all medicines(drugs)available for the treatment of diseases are derived from natural products(from plants,animals,bacteria and fungi)or are derivatives of natural products.The aim of this research was to scientifically identify and supply tangible documentation on these two plants employed in the traditional medicine.From November 2020 to February 2021,an ethnobotanical survey was conducted within four markets at the south of Benin,employing a semi-structured questionnaire.Two hundred respondents including 80%of females and 20%of males were interviewed.The 2 studied plants are mainly used for different types of sickness related to infections.Females’herbalists are the most represented.From this research,it appears that the 2 plants are widely used for the treatment of severe infections.On the market,95%of the leafy steam are sold against 5%of the roots for both plants.The main preparation way is decoction.Oral use is reported to be common in all region.The value of samples sold varies from 200F CFA(Financial Cooperation of Africa)to 1000F CFA.The decoctions are usually obtained through one of a mix of different types of plants.Traditional knowledge is transmitted from one generation to another by oral education.Till today there was no record found.During our study,we did no record prohibition or side effect related to these plants’use.These medicinal plants occupy a crucial place within the therapeutic arsenal of west Africa.Our results constitute a vital tool to determine the true potentials of these plants.These results could lead to new improved traditional medicine.
文摘Fish is rich </span><span style="font-size:10.0pt;font-family:"">in</span><span style="font-size:10.0pt;font-family:""> essential nutrients but can remain a potential source of food poisoning. A study was</span><span style="font-size:10.0pt;font-family:"">,</span><span style="font-size:10.0pt;font-family:""> therefore</span><span style="font-size:10.0pt;font-family:"">,</span><span style="font-size:10.0pt;font-family:""> carried out with the general objective of highlighting the probable biological and chemical dangers associated with the <span>consumption of fish and freshwater fruits from </span></span><span style="font-size:10.0pt;font-family:"">the </span><span style="font-size:10.0pt;font-family:"">Guéssabo river. The</span><span style="font-size:10.0pt;font-family:""> present study was conducted on 50 carp fish, 50 mackerel f</span><span style="font-size:10.0pt;font-family:"">i</span><span style="font-size:10.0pt;font-family:"">sh, 50 frog</span><span style="font-size:10.0pt;font-family:"">s</span><span style="font-size:10.0pt;font-family:""> and 50 mol<span>lusks caught in the Guéssabo River. Microorganisms were enumerated by</span> conventional tests and heavy metals were investigated by Inductively Coupled Plasma Optical Emission Spectrometry. The enumeration showed high levels of thermotolerant Coliforms (2.5 × 10<sup>4</sup>), Enterobacteriaceae (3.4 × 10<sup>4</sup>), <i>S</i>. <i>aureus</i> (2.3 × 10<sup>3</sup>) and Yeast/Molds (2.1 × 10<sup>4</sup>), in all samples the standards were not in conformity with the required standards. High levels of Al (7230 μg/kg) and Cd (21.57 μg/kg) were observed in carp fish and these values are <span>above the standard. In conclusion</span></span><span style="font-size:10.0pt;font-family:"">,</span><span style="font-size:10.0pt;font-family:""> the fish caught in the Guéssabo River</span><span style="font-size:10.0pt;font-family:""> could be a health risk factor for the consumer.
