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Assessment of the Bemisia tabaci C YP6CM l vQ transcript and protein levels in laboratory and field-derived imidacloprid-resistant insects and cross-metabolism potential of the recombinant enzyme 被引量:7
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作者 Emmanouil Roditakis Evangelia Morou +5 位作者 Anastasia Tsagkarakou Maria Riga Ralf Nauen Mark Paine Shai Morin John Vontas 《Insect Science》 SCIE CAS CSCD 2011年第1期23-29,共7页
Over-expression of the cytochrome P450 CYP6CM1 gene has been associated with imidacloprid resistance in a number of Q and B biotype Bemisia tabaci laboratory strains from distinct geographical origins worldwide. We re... Over-expression of the cytochrome P450 CYP6CM1 gene has been associated with imidacloprid resistance in a number of Q and B biotype Bemisia tabaci laboratory strains from distinct geographical origins worldwide. We recently demonstrated that the Q biotype version of the CYP6CM 1 protein (CYP6CMlvQ) is capable of metabolizing imida- cloprid. Here, we show that the levels of BtCYP6CMlvQ were also elevated in laboratory- resistant strains and field-derived populations, with variable imidacloprid resistance levels, collected in Crete. High levels of CYP6CMlvQ transcripts were also determined in survivors of a heterogeneous field population, after exposure to discriminating imidacloprid dosage. Using peptide antibody-based detection assays, we demonstrated that in line with transcriptional data, the CYP6CMlvQ protein levels were higher in imidacloprid-resistant insects, which further implicates the gene as the causal factor of resistance. Finally, assess- ment of the cross-metabolism potential of CYP6CMlvQ against additional neonicotinoid molecules used for B. tabaci control revealed that clothianidin and thiacloprid, but not acetamiprid or thiamethoxam, are metabolized by the recombinant enzyme in vitro. 展开更多
关键词 DETOXIFICATION NEONICOTINOIDS peptide antibodies P450
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Growth performance,welfare traits and meat characteristics of broilers fed diets partly replaced with whole Tenebrio molitor larvae
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作者 Stylianos Vasilopoulos Ilias Giannenas +5 位作者 Soumela Savvidou Eleftherios Bonos Christos I.Rumbos Elias Papadopoulos Paschalis Fortomaris Christos G.Athanassiou 《Animal Nutrition》 SCIE CAS CSCD 2023年第2期90-100,共11页
The role of insects in animal nutrition has been reconsidered during recent years,paving the way for an increasing market for edible insects.Their protein and amino acid balance make them a promising source of protein... The role of insects in animal nutrition has been reconsidered during recent years,paving the way for an increasing market for edible insects.Their protein and amino acid balance make them a promising source of protein for replacing high value proteins.Yellow mealworm,Tenebrio molitor L.(TM;Coleoptera:Tenebrionidae)larvae,have shown positive effects on broiler performance in several research studies and have a strong potential as a sustainable alternative protein source for monogastric animals.This study aimed to assess the effect of replacing various ratios of basal diets with T.molitor larvae on broiler performance as well as on several meat and welfare characteristics.For the study,120 one-day-old male chicks(Ross 308)were randomly allocated in 3 treatments and 4 replications(10 birds per pen).Birds of the control group(basal diet)were fed with typical commercial maize and soymeal-based rations in mash form.The other 2 groups were treated with the same diet,after replacing 5%and 10%with dried TM larvae,respectively.On d 35(end of trial),meat samples were collected and analysed.Body weight,feed intake,body weight gain and feed conversion ratio during the periods of 1 to 10 d(starter period),11 to 24 d(grower period),25 to 35 d(finisher period)and 1 to 35 d(total period)were assessed.Podo-dermatitis,diarrhoea,feather score and litter conditions were also assessed during the trial.The results indicated that TM larvae inclusion in the broilers'diet positively affected body weight gain values,as well as the carcass yield,the meat composition and various welfare traits.Additionally,the dietary treatments with TM larvae favourably affected meat composition and colour parameters,whereas there were also some positive effects on lipid and protein oxidation.Saturated fatty acids were decreased by the dietary supplementation whereas the polyunsaturated fatty acids to SFA ratio increased.In general,the study showed that whole TM larvae addition can provide a promising alternative to soybean meal in the diet of broilers,demonstrating a positive impact on growth,welfare and meat characteristics. 展开更多
关键词 Tenebrio molitor larvae BROILER Performance WELFARE Meat trait
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