MicroRNAs(miRNAs),22-nucleotide-long micromanagers that guide the post-transcriptional regulation of a wide range of target genes,can theoretically be used as a diagnostic or therapeutic target for inflammatory reacti...MicroRNAs(miRNAs),22-nucleotide-long micromanagers that guide the post-transcriptional regulation of a wide range of target genes,can theoretically be used as a diagnostic or therapeutic target for inflammatory reaction.In fish,miR-144-5p expression varies dramatically in response to the different bacterial infections and can regulate immunity-related genes to reduce the occurrence of inflammation.In this research,the regulation function of miR-144-5p to the intestinal innate immunity was udied in flounder Paralichthys olivaceus.The flounders were interfered by 2μg g^(-1) miR-144-5p antagomir and their tissues(intestine,liver and spleen)were harvested from the fish at 12 h post-injection.More than 60 million high-quality reads were collected.At 24 hours after miR-144-5p or miR-NC interference,a total of 2704 and 1823 different-expresion genes(DEGs)were identified in comparison with control group,respectively.The DEGs were enriched in a variety of immunity-related signaling pathways,including NOD-like receptor,Wnt and Toll-like receptor signaling pathways,according to GO and KEGG analyses.A total of 503 highly interacting DEGs engaged in 33 immunity-related signaling pathways were discovered using KEGG analyses.Additionally,5 hub genes were found by protein-protein interaction networks,which formed an intricate immune regulation network.Meanwhile,these hub genes were mostly involved in focal adhesion,Wnt signaling pathway,as well as the Intestinal Immune Network for IgT(IgA)Production Pathway.In conclusion,the loss of miR-144-5p can affect immunity-related genes and downstream signaling pathways.Our findings suggest that miR-144-5p is a modulator of gene networks and signaling pathways associated with intestinal innate immunity.展开更多
Long non-coding RNAs(lncRNAs)regulate a variety of biological processes,including sexual reproduction and differentiation.Saccharina japonica,a commercially important brown alga in China,shows remarkable sexual dimorp...Long non-coding RNAs(lncRNAs)regulate a variety of biological processes,including sexual reproduction and differentiation.Saccharina japonica,a commercially important brown alga in China,shows remarkable sexual dimorphism in haploid gametophytes.The sex of Saccharina japonica gametophytes is determined by UV sexual system.However,no results have been reported on the lncRNAs involved in the sex-related gene regulation of S.japonica.This study identified a number of lncRNAs and assessed their expression levels in male and female gametophytes.Among them,a total of 405 lncRNAs and 211 mRNAs showed differential expressions.Furthermore,the functions of target genes of differentially expressed lncRNAs(DELs)differed from those of differentially expressed genes(DEGs),suggesting that lncRNA may interact with other functional proteins,in addition to DEGs,to involve sex regulation in S.japonica.There were 32 and 90 potential cis-regulatory and trans-regulatory interactions between DELsDEGs,respectively.Five of these lncRNAs(LNC_002974,LNC_021059,LNC_038466,LNC_051584,and LNC_027400)interacted with putative male sex determination region(SDR)genes,suggesting that they act as regulators in gametophytes'sex regulation potentially.Findings from this study contribute to our understanding of the roles of lncRNAs in sex differentiation and lay the foundation for functional studies of candidate lncRNAs in the future.展开更多
Fish embryos are widely used as models in toxicology,drug development,and human disease research because of their high sensitivity,observability,and operability,providing the basis for an in-depth understanding of the...Fish embryos are widely used as models in toxicology,drug development,and human disease research because of their high sensitivity,observability,and operability,providing the basis for an in-depth understanding of the embryogenesis.Increasing studies have indicated that birth defects are hereditary.In this study,we used Oryzias melastigma as a model to conduct a study of 185-day embryogenesis and observed self-induced non-pathological abnormal embryogenesis.O.melastigma experienced pre-puberty stage,adolescence stage,and senescence stage,and individuals produced up to 102 eggs per day.However,the fecundity was markedly reduced during the senescent stage.During the active egg and blastodisc stages,pseudo-fertilization and pseudo-blastocysts were observed.During cleavage at the 4-to 32-cell stages,we observed blastomeres separation or dislocation.Excessively separated blastomeres formed double blastoderms,eventually resulting in conjoined twins.During the blastula stage,we observed abnormally increased cell volume,narrowed and elongated blastocysts,and abnormally coated blastoderms.At the organogenesis stage,we observed abnormal numbers of Kupff er’s vesicles and conjoined twins.Abnormality in the location and number of oil droplets were observed in various development stages.Abnormal development was more commonly observed in fertilized eggs produced by broodstock in pre-puberty or senescence stages,which is probably related to the age of fish and the egg quality.This study can provide the materials for comparative analysis in toxicological and molecular studies of O.melastigma,and may provide evidence for other economic fish that produce sticky eggs.展开更多
To investigate the effect of reduced salinity on diatoms’ capacity to cope with changing ultraviolet radiation(U VR) and photosynthetically active radiation(PAR),Skeletonema costatum was grown in a range of salinity(...To investigate the effect of reduced salinity on diatoms’ capacity to cope with changing ultraviolet radiation(U VR) and photosynthetically active radiation(PAR),Skeletonema costatum was grown in a range of salinity(15,25,and 35).The photo system Ⅱ(PSⅡ) function was analyzed by increasing PAR and UVR to mimic a mixing event in turbulent waters.The re sults show that high UVR exposure significantly reduced PSII activity,especially in cells grown at low salinity.UVR,but not salinity,stimulated the ’removal’ rate of PSII protein PsbA.Salinity alone,in the range of 15 to 35,did not regulate PSⅡ acceptor region;however,the low salinity+UVR treatment decreased the energy flux for electron transport per PSⅡ reaction center in S.costatum.It showed that low salinity exacerbated the damaging effect of UVR on PSⅡ function in S.costatum by suppressing Psb A protein synthe sis and modifying the photochemistry of PSⅡ.Although higher catalase(CAT) activity and NPQs were induced,they were unable to prevent the combined damage effect of low salinity+UVR.Our findings indicate that reduced salinity and increased UVR potentially affect the abundance and distribution of S.costatum with the escalation of climate disturbances.展开更多
Trimethylphenol is an organic toxic byproduct of industrial process,which is difficult to be eliminated through conventional degradation without harsh conditions.In this work,a sulfite-based oxidation process activate...Trimethylphenol is an organic toxic byproduct of industrial process,which is difficult to be eliminated through conventional degradation without harsh conditions.In this work,a sulfite-based oxidation process activated by ZnO-embedded hydrogel was studied for the degradation of 2,4,6-trimethylphenols in the ambient conditions.The ZnO/Na_(2)SO_(3)oxidative system can effectively degrade trimethylphenol via the generation of radicals such as SO_(4)^(-)·,OH·,and SO_(3)^(−)·.The presence of hydrogel matrix facilitates the distribution and recyclability of ZnO catalysts while maintaining high degradation kinetics and little leaching of metal ions.Results suggest the promising potential of ZnO-hydrogel in wastewater treatment with good performance in terms of pH sensitivity,anion interference,recyclability,etc.The combination of ZnO catalysts,hydrogel,and sulfite-based advanced oxidation process may provide essential support for the current treatment of alkylated phenols with strong potential in the commercial scale-ups.展开更多
基金supported by the National Natural Science Foundation of China(No.32002421)the Advanced Talents Foundation of QAU(No.6651118016)+5 种基金the Natural Science Foundation of Shandong Province(No.ZR2019BC009)the Fish Innovation Team of Shandong Agriculture Research System(No.SDAIT-12-06)the Aquatic Animal Immunologic Agents Engineering Research Center of Shandong Province,Key Research and Development Program in Shandong(No.2018YFJH0703)the Consulting Research Project of Shandong Research Institute,China Engineering Science and Technology Development Strategy(No.202103SDYB08)the‘First Class Fishery Discipline’Programme in Shandong Provincethe special top talent plan‘One Thing One Decision(YishiYiyi)’Programme in Shandong Province,China。
文摘MicroRNAs(miRNAs),22-nucleotide-long micromanagers that guide the post-transcriptional regulation of a wide range of target genes,can theoretically be used as a diagnostic or therapeutic target for inflammatory reaction.In fish,miR-144-5p expression varies dramatically in response to the different bacterial infections and can regulate immunity-related genes to reduce the occurrence of inflammation.In this research,the regulation function of miR-144-5p to the intestinal innate immunity was udied in flounder Paralichthys olivaceus.The flounders were interfered by 2μg g^(-1) miR-144-5p antagomir and their tissues(intestine,liver and spleen)were harvested from the fish at 12 h post-injection.More than 60 million high-quality reads were collected.At 24 hours after miR-144-5p or miR-NC interference,a total of 2704 and 1823 different-expresion genes(DEGs)were identified in comparison with control group,respectively.The DEGs were enriched in a variety of immunity-related signaling pathways,including NOD-like receptor,Wnt and Toll-like receptor signaling pathways,according to GO and KEGG analyses.A total of 503 highly interacting DEGs engaged in 33 immunity-related signaling pathways were discovered using KEGG analyses.Additionally,5 hub genes were found by protein-protein interaction networks,which formed an intricate immune regulation network.Meanwhile,these hub genes were mostly involved in focal adhesion,Wnt signaling pathway,as well as the Intestinal Immune Network for IgT(IgA)Production Pathway.In conclusion,the loss of miR-144-5p can affect immunity-related genes and downstream signaling pathways.Our findings suggest that miR-144-5p is a modulator of gene networks and signaling pathways associated with intestinal innate immunity.
基金supported by the National Natural Science Foundation of China(No.132072962)the Agricultural Industrial Technology System in Shandong Province(No.SDAIT-26)+3 种基金the‘First Class Fishery Discipline’Programme[(2020)3]in Shandong Provincethe Special Talent Programme‘Yishi Yiyi’in Shandong Province,Chinathe National Key R&D Program of China(No.2018YFD0901500)the Shandong Province Agriculture Seed Project(No.2021LZ GC004)。
文摘Long non-coding RNAs(lncRNAs)regulate a variety of biological processes,including sexual reproduction and differentiation.Saccharina japonica,a commercially important brown alga in China,shows remarkable sexual dimorphism in haploid gametophytes.The sex of Saccharina japonica gametophytes is determined by UV sexual system.However,no results have been reported on the lncRNAs involved in the sex-related gene regulation of S.japonica.This study identified a number of lncRNAs and assessed their expression levels in male and female gametophytes.Among them,a total of 405 lncRNAs and 211 mRNAs showed differential expressions.Furthermore,the functions of target genes of differentially expressed lncRNAs(DELs)differed from those of differentially expressed genes(DEGs),suggesting that lncRNA may interact with other functional proteins,in addition to DEGs,to involve sex regulation in S.japonica.There were 32 and 90 potential cis-regulatory and trans-regulatory interactions between DELsDEGs,respectively.Five of these lncRNAs(LNC_002974,LNC_021059,LNC_038466,LNC_051584,and LNC_027400)interacted with putative male sex determination region(SDR)genes,suggesting that they act as regulators in gametophytes'sex regulation potentially.Findings from this study contribute to our understanding of the roles of lncRNAs in sex differentiation and lay the foundation for functional studies of candidate lncRNAs in the future.
基金Supported by the National Key R&D Program of China(No.2018YFC1406406)the Science and Technology Program of Yantai(Nos.2018SFBF084,2019ZDCX018)。
文摘Fish embryos are widely used as models in toxicology,drug development,and human disease research because of their high sensitivity,observability,and operability,providing the basis for an in-depth understanding of the embryogenesis.Increasing studies have indicated that birth defects are hereditary.In this study,we used Oryzias melastigma as a model to conduct a study of 185-day embryogenesis and observed self-induced non-pathological abnormal embryogenesis.O.melastigma experienced pre-puberty stage,adolescence stage,and senescence stage,and individuals produced up to 102 eggs per day.However,the fecundity was markedly reduced during the senescent stage.During the active egg and blastodisc stages,pseudo-fertilization and pseudo-blastocysts were observed.During cleavage at the 4-to 32-cell stages,we observed blastomeres separation or dislocation.Excessively separated blastomeres formed double blastoderms,eventually resulting in conjoined twins.During the blastula stage,we observed abnormally increased cell volume,narrowed and elongated blastocysts,and abnormally coated blastoderms.At the organogenesis stage,we observed abnormal numbers of Kupff er’s vesicles and conjoined twins.Abnormality in the location and number of oil droplets were observed in various development stages.Abnormal development was more commonly observed in fertilized eggs produced by broodstock in pre-puberty or senescence stages,which is probably related to the age of fish and the egg quality.This study can provide the materials for comparative analysis in toxicological and molecular studies of O.melastigma,and may provide evidence for other economic fish that produce sticky eggs.
基金Supported by the Shandong Provincial Natural Science Foundation(Nos.ZR2019MC015,ZR2020QC025,ZR2020MD092)the open project of Rongcheng Marine Industrial Technology Research Institute,Ludong University(No.KF20180001)the Key Technology Research and Development Program of Shandong(No.2019GSF107091)。
文摘To investigate the effect of reduced salinity on diatoms’ capacity to cope with changing ultraviolet radiation(U VR) and photosynthetically active radiation(PAR),Skeletonema costatum was grown in a range of salinity(15,25,and 35).The photo system Ⅱ(PSⅡ) function was analyzed by increasing PAR and UVR to mimic a mixing event in turbulent waters.The re sults show that high UVR exposure significantly reduced PSII activity,especially in cells grown at low salinity.UVR,but not salinity,stimulated the ’removal’ rate of PSII protein PsbA.Salinity alone,in the range of 15 to 35,did not regulate PSⅡ acceptor region;however,the low salinity+UVR treatment decreased the energy flux for electron transport per PSⅡ reaction center in S.costatum.It showed that low salinity exacerbated the damaging effect of UVR on PSⅡ function in S.costatum by suppressing Psb A protein synthe sis and modifying the photochemistry of PSⅡ.Although higher catalase(CAT) activity and NPQs were induced,they were unable to prevent the combined damage effect of low salinity+UVR.Our findings indicate that reduced salinity and increased UVR potentially affect the abundance and distribution of S.costatum with the escalation of climate disturbances.
基金the Natural Science Foundation of Shandong Province(No.ZR2017LB028)the Key Research and Development Program of Shandong Province(Nos.2018GSF118032 and 2022CXGC020415)the Fundamental Research Funds for the Central Universities in China(No.18CX02125A).Y.X.L.is grateful for the Research Subsidy Funds of Marine Science Research Institute of Shandong Province.X.W.C.is grateful for Ministry of Science,Innovation and Universities of Spain with project reference number of PID2020-113809RBC33 and by Junta de Andalucía(Spain)with reference number of PY18-2727.
文摘Trimethylphenol is an organic toxic byproduct of industrial process,which is difficult to be eliminated through conventional degradation without harsh conditions.In this work,a sulfite-based oxidation process activated by ZnO-embedded hydrogel was studied for the degradation of 2,4,6-trimethylphenols in the ambient conditions.The ZnO/Na_(2)SO_(3)oxidative system can effectively degrade trimethylphenol via the generation of radicals such as SO_(4)^(-)·,OH·,and SO_(3)^(−)·.The presence of hydrogel matrix facilitates the distribution and recyclability of ZnO catalysts while maintaining high degradation kinetics and little leaching of metal ions.Results suggest the promising potential of ZnO-hydrogel in wastewater treatment with good performance in terms of pH sensitivity,anion interference,recyclability,etc.The combination of ZnO catalysts,hydrogel,and sulfite-based advanced oxidation process may provide essential support for the current treatment of alkylated phenols with strong potential in the commercial scale-ups.
