Pratylenchus ekrami from maize （Zea mays） roots in Shenyang and luffa （Luffa cylindrica） roots in Dalian, China, are described in this paper. Nematodes from the two areas were identified consistently, and were cha...Pratylenchus ekrami from maize （Zea mays） roots in Shenyang and luffa （Luffa cylindrica） roots in Dalian, China, are described in this paper. Nematodes from the two areas were identified consistently, and were characterized by a heavy cephalic sclerotization, extending posteriorly up to two body annuli, stylet 11-13 p.m long, elongating conoid tail, and becoming thinner from vulva. Males were not found. Pratylenchus ekrami is close to Pratylenchus vulnus, but the most critical characteristics between the two species were the number of lip annuli, stylet size, and shape of stylet knobs. This species is the first reported in China.展开更多
A new record species Heterodera ripae was found in the rhizosphere soil of nettle(Urtica sp.)on the bank of the Mangniu River in Shenyang City,Liaoning Province,China.The new record species can be characterized by vul...A new record species Heterodera ripae was found in the rhizosphere soil of nettle(Urtica sp.)on the bank of the Mangniu River in Shenyang City,Liaoning Province,China.The new record species can be characterized by vulval cone bifenestrate with circular or subcircular semifenestrae,vulval slit length averaging 34.2μm,vulval bridge mostly broad,underbridge weak,bullae absent;the second-stage larvae stylet knobs rather wide and slightly projecting anteriorly,tail length averaging 43.7μm and hyaline region length averaging 22.3μm.展开更多
Pratylenchus ekrami from maize(Zea mays)roots in Shenyang and luffa(Luffa cylindrica)roots in Dalian,China,are described in this paper.Nematodes from the two areas were identified consistently,and were characterized b...Pratylenchus ekrami from maize(Zea mays)roots in Shenyang and luffa(Luffa cylindrica)roots in Dalian,China,are described in this paper.Nematodes from the two areas were identified consistently,and were characterized by a heavy cephalic sclerotization,extending posteriorly up to two body annuli,stylet 11−13μm long,elongating conoid tail,and becoming thinner from vulva.Males were not found.Pratylenchus ekrami is close to Pratylenchus vulnus,but the most critical characteristics between the two species were the number of lip annuli,stylet size,and shape of stylet knobs.This species is the first reported in China.展开更多
From 2011 to 2012, we collected Scutellonema commune from the rhizosphere soil of coconut （cocos nucifera L.） and Scutellonema magniphasma from the rhizosphere soil of Rosa chinensis in Shenyang, China. S. commune w...From 2011 to 2012, we collected Scutellonema commune from the rhizosphere soil of coconut （cocos nucifera L.） and Scutellonema magniphasma from the rhizosphere soil of Rosa chinensis in Shenyang, China. S. commune was characterized by rounded scutella, which was varied from two annules anterior to the anus and five annules posterior. The lateral field was not areolated at the level of scutella. S. magniphasma was characterized by varying scutellum, about 7.0 μm in diameter, in position from four annules posterior to four annules anterior to the anus, and areolated at the level of the scutellum.展开更多
A new record species Heterodera ripae was found in the rhizosphere soil of nettle(Urtica sp.) on the bank of the Mangniu River in Shenyang City,Liaoning Province,China.The new record species can be characterized by vu...A new record species Heterodera ripae was found in the rhizosphere soil of nettle(Urtica sp.) on the bank of the Mangniu River in Shenyang City,Liaoning Province,China.The new record species can be characterized by vulval cone bifenestrate with circular or subcircular semifenestrae,vulval slit length averaging 34.2 μm,vulval bridge mostly broad,underbridge weak,bullae absent;the second-stage larvae stylet knobs rather wide and slightly projecting anteriorly,tail length averaging 43.7 μm and hyaline region length averaging 22.3 μm.展开更多
To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) c...To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-time PCR analyses confirmed the changes in mRNA levels observed in our sequencing analyses. A comparable number of genes were upand down-regulated in response to nematode infection, indicating that down-regulation of some genes might be essential in the plant response to nematodes. Our SAGE results showed significant changes in expression of many unreported genes involved in nematode infection. Approximately 7% of tags mapped to the antisense strand of genes, indicating widespread antisense transcription.展开更多
基金supported by China Agriculture Research System(CARS-04)
文摘Pratylenchus ekrami from maize （Zea mays） roots in Shenyang and luffa （Luffa cylindrica） roots in Dalian, China, are described in this paper. Nematodes from the two areas were identified consistently, and were characterized by a heavy cephalic sclerotization, extending posteriorly up to two body annuli, stylet 11-13 p.m long, elongating conoid tail, and becoming thinner from vulva. Males were not found. Pratylenchus ekrami is close to Pratylenchus vulnus, but the most critical characteristics between the two species were the number of lip annuli, stylet size, and shape of stylet knobs. This species is the first reported in China.
基金Agro-scientific Research in the public Interest(200903040-03)China Agriculture Research System(CARS-04)。
文摘A new record species Heterodera ripae was found in the rhizosphere soil of nettle(Urtica sp.)on the bank of the Mangniu River in Shenyang City,Liaoning Province,China.The new record species can be characterized by vulval cone bifenestrate with circular or subcircular semifenestrae,vulval slit length averaging 34.2μm,vulval bridge mostly broad,underbridge weak,bullae absent;the second-stage larvae stylet knobs rather wide and slightly projecting anteriorly,tail length averaging 43.7μm and hyaline region length averaging 22.3μm.
基金This work was supported by China Agriculture Research System(CARS-04)。
文摘Pratylenchus ekrami from maize(Zea mays)roots in Shenyang and luffa(Luffa cylindrica)roots in Dalian,China,are described in this paper.Nematodes from the two areas were identified consistently,and were characterized by a heavy cephalic sclerotization,extending posteriorly up to two body annuli,stylet 11−13μm long,elongating conoid tail,and becoming thinner from vulva.Males were not found.Pratylenchus ekrami is close to Pratylenchus vulnus,but the most critical characteristics between the two species were the number of lip annuli,stylet size,and shape of stylet knobs.This species is the first reported in China.
基金supported by the National Natural Science Foundation of China(31171823)
文摘From 2011 to 2012, we collected Scutellonema commune from the rhizosphere soil of coconut （cocos nucifera L.） and Scutellonema magniphasma from the rhizosphere soil of Rosa chinensis in Shenyang, China. S. commune was characterized by rounded scutella, which was varied from two annules anterior to the anus and five annules posterior. The lateral field was not areolated at the level of scutella. S. magniphasma was characterized by varying scutellum, about 7.0 μm in diameter, in position from four annules posterior to four annules anterior to the anus, and areolated at the level of the scutellum.
基金Special Fund for Agro-scientific Research in the public Interest (200903040-03)China Agriculture Research System(CARS-04)
文摘A new record species Heterodera ripae was found in the rhizosphere soil of nettle(Urtica sp.) on the bank of the Mangniu River in Shenyang City,Liaoning Province,China.The new record species can be characterized by vulval cone bifenestrate with circular or subcircular semifenestrae,vulval slit length averaging 34.2 μm,vulval bridge mostly broad,underbridge weak,bullae absent;the second-stage larvae stylet knobs rather wide and slightly projecting anteriorly,tail length averaging 43.7 μm and hyaline region length averaging 22.3 μm.
基金supported by the National Natural Science Foundation of China (30392100)
文摘To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-time PCR analyses confirmed the changes in mRNA levels observed in our sequencing analyses. A comparable number of genes were upand down-regulated in response to nematode infection, indicating that down-regulation of some genes might be essential in the plant response to nematodes. Our SAGE results showed significant changes in expression of many unreported genes involved in nematode infection. Approximately 7% of tags mapped to the antisense strand of genes, indicating widespread antisense transcription.
