Trincadeira and Aragonez are two important grapevine cultivars in the Iberian Peninsula, used for high quality red wines production. Both cultivars are strongly affected by fungal diseases, with consequent high loses ...Trincadeira and Aragonez are two important grapevine cultivars in the Iberian Peninsula, used for high quality red wines production. Both cultivars are strongly affected by fungal diseases, with consequent high loses on plant productivity and fruit quality. A successful protocol for plant regeneration via somatic embryogenesis (SE) was established for both cultivars allowing further plant improvement based on gene transfer technology. Several factors were evaluated during the three different phases which characterize an SE plant regeneration protocol. The culture room temperature during the induction phase, a parameter usually accepted as standard by most researchers, proved in these trials to significantly affect the embryo induction rates. Concerning embryo conversion, it was specially affected by the embryo developmental stage, by the intensity and duration of the chilling treatment and by the supplementation of conversion culture medium with activated charcoal. The responses obtained, both for induction and conversion, proved to be highly genotype dependent. Calli structure, as well as embryo integrity, was histologically observed, allowing to characterize embryonic and non-embryonic masses and to identify abnormalities on embryo development.展开更多
ABA receptors(PYR/PYL/RCAR)play a central role in the water loss control of plants.A previous report indicated that TaPYL4 is a critical gene in wheat that improves grain production under drought conditions and increa...ABA receptors(PYR/PYL/RCAR)play a central role in the water loss control of plants.A previous report indicated that TaPYL4 is a critical gene in wheat that improves grain production under drought conditions and increases water use efficiency.In this study,we analyzed the sequence polymorphisms and genetic effects of TaPYL4s.Based on isolated TaPYL4 genes from chromosomes 2A,2B and 2D,three haplotypes were detected in the promoter region of TaPYL4-2A,and two haplotypes were present in TaPYL4-2B and TaPYL4-2D,respectively.Marker/trait association analysis indicated that TaPYL4-2A was significantly associated with plant height in 262 Chinese wheat core collection accessions,as well as the drought tolerance coefficient(DTC)for plant height in 239 wheat varieties from Shanxi Province in multiple environments.However,the frequencies of favored drought-tolerant haplotype TaPYL4-2A-Hap2 were considerably low,accounting for only 10%,and lines with this certain Hap could be reserved in the breeding program.TaPYL4-2B was significantly associated with grain number,and the favored haplotype TaPYL4-2B-Hap1 was the dominant allele of above 90% in the collection.For TaPYL4-2D,there were no significant differences in these traits between the two haplotypes in either of the two panels.These results indicate that variation might lead to functional differentiation among the homoeologs and the haplotypes had undergone artificial selection during breeding.Two molecular markers developed to distinguish these haplotypes could be used for breeding in water-limited regions.展开更多
Legumes, with their unique ability to fix atmo- spheric nitrogen, play a vital role in ensuring future food security and mitigating the effects of climate change because they use less fossil energy and produce less gr...Legumes, with their unique ability to fix atmo- spheric nitrogen, play a vital role in ensuring future food security and mitigating the effects of climate change because they use less fossil energy and produce less greenhouse gases compared with N-fertilized systems. Grain legumes are second only to cereal crops as a source of human and animal food, and they contribute approximately one third of the protein consumed by the human population. The productivityof seed crops, such as grain legumes, is dependent on flowering. Despite the genetic variation and importance of flowering in legume production, studies of the molecular pathways that control flowering in legumes are limited. Recent advances in genomics have revealed that legume flowering pathways are divergent from those of such model species as Arabidopsis thaliana. Here, we discuss the current understanding of flowering time regulation in legumes and highlight the unique and conserved features of floral evocation in legumes.展开更多
It is vital to ramp up crop production dramatically by 2050 due to the increasing global population and demand for food.However, with the climate change projections showing that droughts and heatwaves becoming common ...It is vital to ramp up crop production dramatically by 2050 due to the increasing global population and demand for food.However, with the climate change projections showing that droughts and heatwaves becoming common in much of the globe,there is a severe threat of a sharp decline in crop yields. Thus, developing crop varieties with inbuilt genetic tolerance toenvironmental stresses is urgently needed. Selective breeding based on genetic diversity is not keeping up with the growingdemand for food and feed. However, the emergence of contemporary plant genetic engineering, genome-editing, and syntheticbiology offer precise tools for developing crops that can sustain productivity under stress conditions. Here, we summarize thesystems biology-level understanding of regulatory pathways involved in perception, signalling, and protective processesactivated in response to unfavourable environmental conditions. The potential role of noncoding RNAs in the regulation ofabiotic stress responses has also been highlighted. Further, examples of imparting abiotic stress tolerance by geneticengineering are discussed. Additionally, we provide perspectives on the rational design of abiotic stress tolerance throughsynthetic biology and list various bioparts that can be used to design synthetic gene circuits whose stress-protective functionscan be switched on/off in response to environmental cues.展开更多
Pollen allergens are among the most abundantly transcribed and translated products in the life history of plants, and particularly grasses. To identify different pollen allergens in rice, putative allergens were ident...Pollen allergens are among the most abundantly transcribed and translated products in the life history of plants, and particularly grasses. To identify different pollen allergens in rice, putative allergens were identified in the rice genome and their expression characterized using the Affymetrix 57K rice GeneChip microarray. Among the most abundant pollen-specific candidate transcripts were Ory s 1 beta-expansin, Ory s 2, Ory s 7 EF hand, Q ry s 11, Ory s 12 profilin A, Ory s 23, glycosyl hydrolase family 28 （polygalacturonase）, and FAD binding proteins. Highly expressed pollen proteins are frequently present in multiple copy numbers, sometimes with mirror images located on nearby regions of the opposite DNA strand. Many of these are intronless and inserted as copies that retain nearly exact copies of their regulatory elements. Ory s 23 reflects low variability and high copy number, suggesting recent gene amplification. Some copies contain pseudogenes, which may reflect their origin through activity of retrotransposition; some putative allergenic sequences bear fusion products with repeat sequences of transposable elements （LTRs）. The abundance of nearby repetitive sequences, activation of transposable elements, and high production of mRNA transcripts appear to coincide in pollen and may contribute to a syndrome in which highly transcribed proteins may be copied and inserted with streamlined features for translation, including grouping and removal of introns.展开更多
文摘Trincadeira and Aragonez are two important grapevine cultivars in the Iberian Peninsula, used for high quality red wines production. Both cultivars are strongly affected by fungal diseases, with consequent high loses on plant productivity and fruit quality. A successful protocol for plant regeneration via somatic embryogenesis (SE) was established for both cultivars allowing further plant improvement based on gene transfer technology. Several factors were evaluated during the three different phases which characterize an SE plant regeneration protocol. The culture room temperature during the induction phase, a parameter usually accepted as standard by most researchers, proved in these trials to significantly affect the embryo induction rates. Concerning embryo conversion, it was specially affected by the embryo developmental stage, by the intensity and duration of the chilling treatment and by the supplementation of conversion culture medium with activated charcoal. The responses obtained, both for induction and conversion, proved to be highly genotype dependent. Calli structure, as well as embryo integrity, was histologically observed, allowing to characterize embryonic and non-embryonic masses and to identify abnormalities on embryo development.
基金supported by the Agricultural Science Research of Shanxi Academy of Agricultural Sciences,China(YZGC013 and YCX2020BH2)the Key Research and Development Program of Shanxi Province,China(201803D421021 and 201903D221074)。
文摘ABA receptors(PYR/PYL/RCAR)play a central role in the water loss control of plants.A previous report indicated that TaPYL4 is a critical gene in wheat that improves grain production under drought conditions and increases water use efficiency.In this study,we analyzed the sequence polymorphisms and genetic effects of TaPYL4s.Based on isolated TaPYL4 genes from chromosomes 2A,2B and 2D,three haplotypes were detected in the promoter region of TaPYL4-2A,and two haplotypes were present in TaPYL4-2B and TaPYL4-2D,respectively.Marker/trait association analysis indicated that TaPYL4-2A was significantly associated with plant height in 262 Chinese wheat core collection accessions,as well as the drought tolerance coefficient(DTC)for plant height in 239 wheat varieties from Shanxi Province in multiple environments.However,the frequencies of favored drought-tolerant haplotype TaPYL4-2A-Hap2 were considerably low,accounting for only 10%,and lines with this certain Hap could be reserved in the breeding program.TaPYL4-2B was significantly associated with grain number,and the favored haplotype TaPYL4-2B-Hap1 was the dominant allele of above 90% in the collection.For TaPYL4-2D,there were no significant differences in these traits between the two haplotypes in either of the two panels.These results indicate that variation might lead to functional differentiation among the homoeologs and the haplotypes had undergone artificial selection during breeding.Two molecular markers developed to distinguish these haplotypes could be used for breeding in water-limited regions.
基金supported by the Australian Research Council in the form of the ARC Centre of Excellence for Integrative Legume Research (CE0348212)McKenzie Postdoctoral Fellowship by the University of Melbourne
文摘Legumes, with their unique ability to fix atmo- spheric nitrogen, play a vital role in ensuring future food security and mitigating the effects of climate change because they use less fossil energy and produce less greenhouse gases compared with N-fertilized systems. Grain legumes are second only to cereal crops as a source of human and animal food, and they contribute approximately one third of the protein consumed by the human population. The productivityof seed crops, such as grain legumes, is dependent on flowering. Despite the genetic variation and importance of flowering in legume production, studies of the molecular pathways that control flowering in legumes are limited. Recent advances in genomics have revealed that legume flowering pathways are divergent from those of such model species as Arabidopsis thaliana. Here, we discuss the current understanding of flowering time regulation in legumes and highlight the unique and conserved features of floral evocation in legumes.
基金the ARC Discovery Grant DP0988972 and the University of Melbourne Postgraduate Research Scholarship is gratefully acknowledged.
文摘It is vital to ramp up crop production dramatically by 2050 due to the increasing global population and demand for food.However, with the climate change projections showing that droughts and heatwaves becoming common in much of the globe,there is a severe threat of a sharp decline in crop yields. Thus, developing crop varieties with inbuilt genetic tolerance toenvironmental stresses is urgently needed. Selective breeding based on genetic diversity is not keeping up with the growingdemand for food and feed. However, the emergence of contemporary plant genetic engineering, genome-editing, and syntheticbiology offer precise tools for developing crops that can sustain productivity under stress conditions. Here, we summarize thesystems biology-level understanding of regulatory pathways involved in perception, signalling, and protective processesactivated in response to unfavourable environmental conditions. The potential role of noncoding RNAs in the regulation ofabiotic stress responses has also been highlighted. Further, examples of imparting abiotic stress tolerance by geneticengineering are discussed. Additionally, we provide perspectives on the rational design of abiotic stress tolerance throughsynthetic biology and list various bioparts that can be used to design synthetic gene circuits whose stress-protective functionscan be switched on/off in response to environmental cues.
文摘Pollen allergens are among the most abundantly transcribed and translated products in the life history of plants, and particularly grasses. To identify different pollen allergens in rice, putative allergens were identified in the rice genome and their expression characterized using the Affymetrix 57K rice GeneChip microarray. Among the most abundant pollen-specific candidate transcripts were Ory s 1 beta-expansin, Ory s 2, Ory s 7 EF hand, Q ry s 11, Ory s 12 profilin A, Ory s 23, glycosyl hydrolase family 28 （polygalacturonase）, and FAD binding proteins. Highly expressed pollen proteins are frequently present in multiple copy numbers, sometimes with mirror images located on nearby regions of the opposite DNA strand. Many of these are intronless and inserted as copies that retain nearly exact copies of their regulatory elements. Ory s 23 reflects low variability and high copy number, suggesting recent gene amplification. Some copies contain pseudogenes, which may reflect their origin through activity of retrotransposition; some putative allergenic sequences bear fusion products with repeat sequences of transposable elements （LTRs）. The abundance of nearby repetitive sequences, activation of transposable elements, and high production of mRNA transcripts appear to coincide in pollen and may contribute to a syndrome in which highly transcribed proteins may be copied and inserted with streamlined features for translation, including grouping and removal of introns.
