With the development of Guizhou’s economy and society, higher vocational medical education in Guizhou has developed rapidly, making it its mission to cultivate practical and skilled talents oriented to the grassroots...With the development of Guizhou’s economy and society, higher vocational medical education in Guizhou has developed rapidly, making it its mission to cultivate practical and skilled talents oriented to the grassroots and serving for frontline. However, due to the social environment, policy environment and insufficient funding, many difficulties and problems are faced. It is necessary to have a unified management throughout the province, rationally lay out higher vocational colleges and specialties, and promote the healthy and rapid development of medical higher vocational education in Guizhou with advanced concepts, proper policies, and sufficient funds in place, making higher vocational medical education in Guizhou enter a benign development period.展开更多
Objective:In this study,we examined the effects of Shizhenqing granule(SZQG)on hypothalamicpituitary-adrenal(HPA)axis and serum inflammatory factors in a rat model of chronic eczema,in order to explore the mechanism o...Objective:In this study,we examined the effects of Shizhenqing granule(SZQG)on hypothalamicpituitary-adrenal(HPA)axis and serum inflammatory factors in a rat model of chronic eczema,in order to explore the mechanism of action of SZQG in treatment of this disease.Methods:Sixty SpragueeDawley male rats were randomly divided into six groups(with 10 rats per group):blank group;model group;positive control group(prednisone);and the low-,medium-,and high-dose SZQG groups.Except for the blank group,rats in all other groups were treated with 2,4-dinitrochlorobenzene to induce chronic eczema.These rats were administered prednisone or SZQG for 7 consecutive days after successful establishment of the chronic eczema model,and samples were collected 12 h after the last administration.The degree of skin lesions and the changes in serum levels of corticotropin-releasing hormone(CRH),adrenocorticotropic hormone(ACTH),cortisol(CORT),interleukin(IL)-4,interferon(IFN)-g,IL-25,and IL-31 among the groups were compared.Results:SZQG effectively increased the levels of CRH,ACTH,and CORT,which decreased in the serum of rats with chronic eczema,stimulated the function of the HPA axis,and promoted the expression of glucocorticoids.SZQG reduced the serum levels of inflammatory factors including IL-4,IL-25 and IL-31,which were overexpressed in rats with chronic eczema,and increased those of anti-inflammatory factor IFN-g,thereby alleviating the inflammatory symptoms and itching,and ameliorating the clinical symptoms of chronic eczema.Conclusion:SZQG effectively alleviates skin lesions in the chronic eczema rat model by stimulating the function of the HPA axis.展开更多
OBJECTIVE:To evaluate the efficacy of meridian massage on motor function after a stroke.METHODS:This systematic review and Meta-analysis of randomised(RCTs)and quasi-randomised controlled trials(q RCTs)was performed t...OBJECTIVE:To evaluate the efficacy of meridian massage on motor function after a stroke.METHODS:This systematic review and Meta-analysis of randomised(RCTs)and quasi-randomised controlled trials(q RCTs)was performed to determine the effects of meridian massage on motor dysfunction following a stroke.Articles published until December 2020 related to stroke and meridian massage from National Library of Medicine,Cochrane Central Register of Controlled Trials,Embase,Korea Med,Japan science and Technology Agency,China National Knowledge Infrastructure Database,Wanfang Database,Chinese Biomedical Literature Database and China Science and Technology Journal Database were searched.The primary outcomes included Fugl-Mayer Assessment(FMA),modified Barthel Index(m BI)and clinical efficiency,while the secondary outcomes included modified Ashworth(m AS)and Berg Balance Scale(BBS).Two authors independently selected the literature,extracted the data and assessed the risk of bias,after which Meta-analysis with subgroups and sensitivity analysis was conducted.RESULTS:A total of 16 RCTs and 3 q-RCTs involved 1556 patients satisfied the inclusion.Meta-analysis showed that compared to conventional rehabilitation,meridian massage had significantly better FMA[mean difference(MD)=1.16,95%confidence interval(CI):0.43,1.89,P=0.002],FMA-L(MD=3.21,95%CI:1.97,4.45,P=0.00001),m BI(MD=8.87,95%CI:4.24,13.49,P=0.00002),clinical efficiency[relative risk(RR)=1.37,95%CI:1.24,1.51,P<0.00001],m AS(MD=-0.69,95%CI:-1.26,-0.11,P=0.02)and BBS(MD=5.07,95%CI:2.22,7.93,P=0.0005).Subgroup analysis indicated no significant difference between meridian massage and control group when the intervention duration was>8 weeks(MD=0.89,95%CI:-0.64,2.42,P=0.25)but improving motor function in the meridian massage when the intervention duration was≤8 weeks(<4 weeks intervention duration MD=0.84,95%CI:0.59,1.10,P<0.00001;4–8 weeks MD=2.41,95%CI:0.80,4.01,P=0.003).CONCLUSIONS:Meridian massage as an alternative and complementary therapy seems to have short-term benefits in improving post-stroke motor function(particularly in the lower limbs),quality of life,clinical efficiency,and balance stability and muscle spasms.The high heterogeneity of the included studies and the low methodological quality certainly limits the confidence placed in this systematic review and Meta-analysis,warranting future well-designed studies.展开更多
Five characteristics(hair forms,nasal profile,nostril forms,mongoloid fold and upper eyelid fold)were respectively investigated in six nationalities,including the Buyi,Miao,Shui,Maonan,Dong,and Han nationalities in So...Five characteristics(hair forms,nasal profile,nostril forms,mongoloid fold and upper eyelid fold)were respectively investigated in six nationalities,including the Buyi,Miao,Shui,Maonan,Dong,and Han nationalities in Southern Guizhou,China.The gene frequencies of five characteristics in the six nationalities were estimated and compared.The results indicated that:(1)for hair forms and nasal profile,the frequency of the dominant gene was lower than that of their recessive gene,but the opposite was true for nostril forms.(2)Among different nationalities,the difference of gene frequency of the mongoloid fold,nasal profile,hair forms and upper eyelid fold was quite significant,which was followed by that of nostril forms.展开更多
SQUAMOSA promoter binding protein-likes （SPLs） are plant-specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs in Salvia miltiorrhiza （Dan...SQUAMOSA promoter binding protein-likes （SPLs） are plant-specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs in Salvia miltiorrhiza （Danshen）, a significant medicinal plant widely used in Traditional Chinese medicine （TCM） for＆gt;1,700 years and an emerging model plant for TCM studies. Through genome-wide identification and subsequent molecular cloning, we identified a total 15 SmSPLs with divergent sequence features, gene structures, and motifs. Comparative analysis showed sequence conservation between SmSPLs and their Arabidopsis counterparts. A phylogenetic tree clusters SmSPLs into six groups. Many of the motifs identified commonly exist in a group/subgroup, implying their functional redundancy. Eight SmSPLs were predicted and experimental y validated to be targets of miR156/157. SmSPLs were differen-tial y expressed in various tissues of S. milltiorrhiza. The expression of miR156/157-targeted SmSPLs was increased with＆amp;nbsp;the maturation of S. miltiorrhiza, whereas the expression of miR156/157 was decreased, confirming the regulatory roles of miR156/157 in SmSPLs and suggesting the functions of SmSPLs in S. miltiorrhiza development. The expression of miR156/157 was negatively correlated with miR172 during the maturation of S. miltiorrhiza. The results indicate the significance and complexity of SmSPL-, miR156-, and miR172-mediated regula-tion of developmental timing in S. miltiorrhiza.展开更多
文摘With the development of Guizhou’s economy and society, higher vocational medical education in Guizhou has developed rapidly, making it its mission to cultivate practical and skilled talents oriented to the grassroots and serving for frontline. However, due to the social environment, policy environment and insufficient funding, many difficulties and problems are faced. It is necessary to have a unified management throughout the province, rationally lay out higher vocational colleges and specialties, and promote the healthy and rapid development of medical higher vocational education in Guizhou with advanced concepts, proper policies, and sufficient funds in place, making higher vocational medical education in Guizhou enter a benign development period.
基金This study was supported by the Beijing municipal project(2010071620067)Research fund from Qiannan Medical College for Nationalities(QNYZ2018030).
文摘Objective:In this study,we examined the effects of Shizhenqing granule(SZQG)on hypothalamicpituitary-adrenal(HPA)axis and serum inflammatory factors in a rat model of chronic eczema,in order to explore the mechanism of action of SZQG in treatment of this disease.Methods:Sixty SpragueeDawley male rats were randomly divided into six groups(with 10 rats per group):blank group;model group;positive control group(prednisone);and the low-,medium-,and high-dose SZQG groups.Except for the blank group,rats in all other groups were treated with 2,4-dinitrochlorobenzene to induce chronic eczema.These rats were administered prednisone or SZQG for 7 consecutive days after successful establishment of the chronic eczema model,and samples were collected 12 h after the last administration.The degree of skin lesions and the changes in serum levels of corticotropin-releasing hormone(CRH),adrenocorticotropic hormone(ACTH),cortisol(CORT),interleukin(IL)-4,interferon(IFN)-g,IL-25,and IL-31 among the groups were compared.Results:SZQG effectively increased the levels of CRH,ACTH,and CORT,which decreased in the serum of rats with chronic eczema,stimulated the function of the HPA axis,and promoted the expression of glucocorticoids.SZQG reduced the serum levels of inflammatory factors including IL-4,IL-25 and IL-31,which were overexpressed in rats with chronic eczema,and increased those of anti-inflammatory factor IFN-g,thereby alleviating the inflammatory symptoms and itching,and ameliorating the clinical symptoms of chronic eczema.Conclusion:SZQG effectively alleviates skin lesions in the chronic eczema rat model by stimulating the function of the HPA axis.
基金Supported by the National Natural Science Foundation of China(Based on the Regulation of MIR-206 on BDNF,to Explore the Mechanism of Meridian Massage on Motor Function Reconstruction in rats with Cerebral Infarction,No.8166150430)
文摘OBJECTIVE:To evaluate the efficacy of meridian massage on motor function after a stroke.METHODS:This systematic review and Meta-analysis of randomised(RCTs)and quasi-randomised controlled trials(q RCTs)was performed to determine the effects of meridian massage on motor dysfunction following a stroke.Articles published until December 2020 related to stroke and meridian massage from National Library of Medicine,Cochrane Central Register of Controlled Trials,Embase,Korea Med,Japan science and Technology Agency,China National Knowledge Infrastructure Database,Wanfang Database,Chinese Biomedical Literature Database and China Science and Technology Journal Database were searched.The primary outcomes included Fugl-Mayer Assessment(FMA),modified Barthel Index(m BI)and clinical efficiency,while the secondary outcomes included modified Ashworth(m AS)and Berg Balance Scale(BBS).Two authors independently selected the literature,extracted the data and assessed the risk of bias,after which Meta-analysis with subgroups and sensitivity analysis was conducted.RESULTS:A total of 16 RCTs and 3 q-RCTs involved 1556 patients satisfied the inclusion.Meta-analysis showed that compared to conventional rehabilitation,meridian massage had significantly better FMA[mean difference(MD)=1.16,95%confidence interval(CI):0.43,1.89,P=0.002],FMA-L(MD=3.21,95%CI:1.97,4.45,P=0.00001),m BI(MD=8.87,95%CI:4.24,13.49,P=0.00002),clinical efficiency[relative risk(RR)=1.37,95%CI:1.24,1.51,P<0.00001],m AS(MD=-0.69,95%CI:-1.26,-0.11,P=0.02)and BBS(MD=5.07,95%CI:2.22,7.93,P=0.0005).Subgroup analysis indicated no significant difference between meridian massage and control group when the intervention duration was>8 weeks(MD=0.89,95%CI:-0.64,2.42,P=0.25)but improving motor function in the meridian massage when the intervention duration was≤8 weeks(<4 weeks intervention duration MD=0.84,95%CI:0.59,1.10,P<0.00001;4–8 weeks MD=2.41,95%CI:0.80,4.01,P=0.003).CONCLUSIONS:Meridian massage as an alternative and complementary therapy seems to have short-term benefits in improving post-stroke motor function(particularly in the lower limbs),quality of life,clinical efficiency,and balance stability and muscle spasms.The high heterogeneity of the included studies and the low methodological quality certainly limits the confidence placed in this systematic review and Meta-analysis,warranting future well-designed studies.
文摘Five characteristics(hair forms,nasal profile,nostril forms,mongoloid fold and upper eyelid fold)were respectively investigated in six nationalities,including the Buyi,Miao,Shui,Maonan,Dong,and Han nationalities in Southern Guizhou,China.The gene frequencies of five characteristics in the six nationalities were estimated and compared.The results indicated that:(1)for hair forms and nasal profile,the frequency of the dominant gene was lower than that of their recessive gene,but the opposite was true for nostril forms.(2)Among different nationalities,the difference of gene frequency of the mongoloid fold,nasal profile,hair forms and upper eyelid fold was quite significant,which was followed by that of nostril forms.
基金supported by grants from the Beijing Natural Science Foundation(Grant No.5112026 to S.L.)the Major Scientific and Technological Special Project for Significant New Drugs Creation(Grant No.2012ZX09301002-001-031 to S.L.)+2 种基金the Research Fund for the Doctoral Program of Higher Education of China(20111106110033 to S.L.)the Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT,Grant No.IRT1150)the Program for Xiehe Scholars in Chinese Academy of Medical Sciences&Peking Union Medical College(to S.L.)
文摘SQUAMOSA promoter binding protein-likes （SPLs） are plant-specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs in Salvia miltiorrhiza （Danshen）, a significant medicinal plant widely used in Traditional Chinese medicine （TCM） for＆gt;1,700 years and an emerging model plant for TCM studies. Through genome-wide identification and subsequent molecular cloning, we identified a total 15 SmSPLs with divergent sequence features, gene structures, and motifs. Comparative analysis showed sequence conservation between SmSPLs and their Arabidopsis counterparts. A phylogenetic tree clusters SmSPLs into six groups. Many of the motifs identified commonly exist in a group/subgroup, implying their functional redundancy. Eight SmSPLs were predicted and experimental y validated to be targets of miR156/157. SmSPLs were differen-tial y expressed in various tissues of S. milltiorrhiza. The expression of miR156/157-targeted SmSPLs was increased with＆amp;nbsp;the maturation of S. miltiorrhiza, whereas the expression of miR156/157 was decreased, confirming the regulatory roles of miR156/157 in SmSPLs and suggesting the functions of SmSPLs in S. miltiorrhiza development. The expression of miR156/157 was negatively correlated with miR172 during the maturation of S. miltiorrhiza. The results indicate the significance and complexity of SmSPL-, miR156-, and miR172-mediated regula-tion of developmental timing in S. miltiorrhiza.
