Artificial fishponds play a pivotal role in global aquaculture, serving as a source of livelihood and nourishment for many communities. Ensuring the sustained health and productivity of Fishes in these environments re...Artificial fishponds play a pivotal role in global aquaculture, serving as a source of livelihood and nourishment for many communities. Ensuring the sustained health and productivity of Fishes in these environments relies heavily on water quality management. This assessment was done to determine the water quality of ten artificial fishponds in the south-eastern part of Sierra Leone using twelve physicochemical factors (pH, BOD, EC, TDS, turbidity, COD, Fe<sup>2+</sup>, Mg<sup>2+</sup>, Ca<sup>2+</sup>, NH<sub>3</sub>, , and alkalinity) to find out the Water Quality Index (WQI) and spatial distribution of respective parameters. The assessment of artificial fishponds using WQI and Inverse Distant Weighting (IDW) integration represents a relatively underexplored area within the domain of environmental water resources. The WQI was determined using the “Weighted Arithmetic Water Quality Index’’ method. The results of WQI in the study area range from 65.05 to 147.26. Several locations have water quality deemed unsuitable for consumption, while others range from good to very poor. It is essential to address and improve water quality in locations categorized as unsuitable for consumption and very poor to ensure safe and healthy water sources. It was also clear from the calculation that the smaller the mean concentration value of the pH as compared to the ideal value (7), the smaller the WQI value and the better the water quality. To keep the artificial fishpond water in good condition, mass domestic use should be controlled, and draining of surrounding organic matter should be stopped in ponds Bo_001, Kenema_001, and Kenema_002.展开更多
Background: Due to their delicious taste, high nutritional content, and health benefits, fruit juices are well-known drinks in many countries and are now an essential component of the modern diet. Objective: Determini...Background: Due to their delicious taste, high nutritional content, and health benefits, fruit juices are well-known drinks in many countries and are now an essential component of the modern diet. Objective: Determining the microbiological quality of both packaged and freshly made fruit and milk juices. Method: The spread-plate approach was employed to isolate and count the bacteria. 90 ml of sterile peptone water were blended with 10 ml of well-mixed, packed, and freshly made fruit juices. The samples were sequentially diluted (101 - 105) in accordance with the Indian Manual of Food Microbiological Testing Methods. Results: From eight samples of imported packaged fruit and milk juice, the average of total coliform, staphylococci, and viable bacterial counts were zero, 1.39 × 102, and 2 × 102 CFU/ml, respectively. In contrast, from three samples of locally produced fruit and milk juice, the average of total coliform, staphylococci, and viable bacterial counts were zero, 5.83 × 102, and 2.73 × 103 CFU/ml, respectively. Four samples of handmade prepared fruit and milk juices had a mean of total coliform, staphylococci, and viable bacterial count of 1.441 × 104, 4.1 × 103, and 2.35 × 105 CFU/ml, respectively. Conclusion: 33.3% of the results from microbiological analysis of freshly made fruit and milk juices met the permissible range of the Revised Microbiological Standards for Fruit and Vegetables and Their Products, which were published in 2018 and as well as the Hong Kong Center for Food Safety, whereas 66.7% of the microbiological analyses of freshly prepared fruit and milk juices were above the permissible reference range of GSO standard 2000. 12.5% of the investigated imported and packed fruits and milk juices had one failed test (TSC), which was above the acceptable limit, 87.5% of the tested samples of fruit and milk juices fulfilled the necessary standards of TCC, TVBC, and TSC. 100% of the tested locally manufactured fruit and milk juices complied with TSC, TCC, and TVBC requirements. All investigations showed that freshly made fruit and milk juices were heavily contaminated (Total viable bacterial count, total coliform count, and total staphylococcus count). .展开更多
Kalanchoe pinnata (Lam.) Pers. is known as a plant that has many special benefits such as anti-inflammatory and antibacterial. The present study was carried out to perform a phytochemicals study and evaluate the antim...Kalanchoe pinnata (Lam.) Pers. is known as a plant that has many special benefits such as anti-inflammatory and antibacterial. The present study was carried out to perform a phytochemicals study and evaluate the antimicrobial and anti-inflammatory activity of the hydroethanolic extract of Kalanchoe pinnata (Lam.) Pers. leaves. After phytochemicals screening, the content of phenolic compounds, proanthocyanidol and flavonoids in the extract of this plant was determined spectrophotometrically. Antimicrobial activity was assessed using the micro-dilution technique on 96-well plates in liquid medium, combined with agar spreading. Anti-inflammatory activity was assessed using the 1% carrageenan induced rat paw oedema model. Phytochemical screening revealed the presence of alkaloids, saponins, triterpenes and sterols, phenols and flavonoids in the plant extract in varying proportions. The extract contained (0.049 ± 0.03 µg EAG/mg extract) total polyphenols, (0.215 ± 0.025 µg CE/mg extract) proanthocyanidins and (385.435 ± 0.0328 µg ER/mg ES) flavonoids. The hydroethanolic extract of the leaves of this plant inhibited the in vitro growth of the microbial strains studied to varying degrees. The MIC of the extract varied from 12.5 to 25 mg/mL and the BMC from 12.5 to 50 mg/mL. The plant did not show any activity on 1% carrageenan-induced rat paw edema.展开更多
This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated Decembe...This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated December 17, 2001 and n˚407 dated October 3, 2008 respectively. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazolyl-2-yl-2,5-diphenyltetrazolium bromide (MTT) test. No significant toxicity was observed after 14 and 28 days, although a dose-dependent decrease in creatinemia was observed in male rats (the recipe to be used to moderate creatinine levels). Cytotoxicity was without effect on NCM 365 cells. The results obtained justify the use of the recipe in traditional medicine.展开更多
Little is known about the impact of direct sowing under vegetation cover on the production and quality of New Rice for Africa （NERICA） on poor oxisol. In this study, two NERICA varieties （NERICA 3 and NERICA 8） w...Little is known about the impact of direct sowing under vegetation cover on the production and quality of New Rice for Africa （NERICA） on poor oxisol. In this study, two NERICA varieties （NERICA 3 and NERICA 8） were grown under tropical oxisol soil with very low nutrient contents. Four cultivation systems were used in completely randomized block design, including plowing （control）, unplowed soil with dead vegetation cover （DVC）, unplowed soil with live vegetation cover （LVC） and unplowed soil with mixed vegetation cover （MVC）. DVC significantly improved the exponential growth of NERICAs. NERICA 3 was the more productive （2.16–3.05 t/hm2） compared with NERICA 8 （0.71–1.21 t/hm2）. Cultivation systems improved the nutritional quality of NERICAs. The total protein content of NERICA 3 under DVC and MVC was 84.8% and 75.0% higher than control, respectively. The total soluble carbohydrate contents of NERICA 8 under LVC and MVC was 73.2% and 57.3% higher than control, respectively. These results suggested that conservative approach like direct sowing on unplowed soil with vegetation cover systems can improve the nutritional quality of rainfed NERICAs and their sustainable production under poor oxisol soil in sub-Saharan Africa.展开更多
The objective of this study is to assess the quality of the artesian borehole water consumed by the population of the village of Dogbo Ahomey in order to prevent pathologies on the populations. Methodology and Results...The objective of this study is to assess the quality of the artesian borehole water consumed by the population of the village of Dogbo Ahomey in order to prevent pathologies on the populations. Methodology and Results: The household surveys were supplemented by a campaign to analyze the water samples in the laboratory. The samples were analyzed according to the standardized methods of the American Public Health Association (APHAAWWA-WPCF, 1994). A total of twenty (20) physico-chemical parameters and four (04) microbiological parameters were used to assess the quality of the different water samples. The results of the physico-chemical analysis showed that the water samples studied present values that are below the standards recommended by the WHO and by the Beninese standards for the quality of drinking water (Standards, 2001). From a microbiological analysis point of view, of the four samples studied, two show strong pollution by germs. This could be a significant health risk for households who take water from these sources to meet their needs.展开更多
The Brazilian Ministry of Health recommends that by 8 months of age, children should eat the same types of foods consumed by the other members of the family. Thus, this study sought to evaluate whether the nutritional...The Brazilian Ministry of Health recommends that by 8 months of age, children should eat the same types of foods consumed by the other members of the family. Thus, this study sought to evaluate whether the nutritional composition of meals specially prepared (SM) for children aged 7 to 18 months in low-income families was superior to that of meals adapted (AM) from the family’s table foods. Protein, fat, carbohydrate, energy, dietary fiber, iron, sodium and sodium chloride values, were determined by chemical analyses and compared to dietetic guidelines. The infants’ hemoglobin levels were also investigated. In total, sixty samples of the infants’ lunch meal (51 AM and 9 SM) were taken for during a home visit. The values of protein, fat, carbohydrate, dietary fiber and energy of the AM were significantly lower, while the sodium and sodium chloride values were significantly higher, compared to those of the SM. The AM also contained significantly more water. No differences were seen with regard to iron values. Sodium chloride amounted for most of the sodium content. Neither the SM nor AM was adequate in terms of iron and sodium. All SM were adequate for protein and fat, whereas AM showed significantly more samples with inadequate energy levels. SM fell within the Acceptable Macronutrient Distribution Range, while AM fell below the lower value for fat and slightly above the upper value for carbohydrate. The prevalence of anemia was 60% in the study population (36/60). In conclusion, meals adapted from the family’s table foods showed a lower nutrient density and a less balanced macronutrient range when compared to meals specially prepared for infants. The main nutritional shortcomings, for both AM and SM, were the extremely low content of iron and the high content of sodium.展开更多
Background Under K deficiency the uptake and distribution pattern in plant cells is mediated through different transport proteins and channels which were controlled by specific gene family.Therefore,a hydroponic exper...Background Under K deficiency the uptake and distribution pattern in plant cells is mediated through different transport proteins and channels which were controlled by specific gene family.Therefore,a hydroponic experiment was conducted under control condition for testing the gene expression pattern of the K transporter under adequate and low K supply levels.After that,a 2-year field experiment was conducted to evaluate five selected cotton cultivars(four K-efficient cultivars,viz.,CIM-554,CYTO-124,FH-142,IUB-2013,and one K non-efficient,BH-212) screened from the initial hydroponics culture experiment and two levels of potassium(0 K_(2)O kg·ha^(-1) and 50 K_(2)O kg·ha^(-1)) were tested under reduced irrigation(50% available water content;50 AWC) and normal irrigation conditions(100% available water content;100 AWC).Result Results revealed that the transcript levels of GhHAK5aD in roots were significantly higher in K^(+) efficient cultivars than that in K^(+) non-efficient cultivars.The GhHAK5aD expression upon K^(+) deficiency was higher in roots but lower in shoots,indicating that GhHAK5aD could have a role in K^(+) uptake in roots,instead of transport of K^(+) from root to shoot.Similarly,under field conditions the cultivar FH-142 showed an increase of 22.3%,4.9%,2.4%,and 1.4% as compared with BH-212,IUB-2013,CYTO-124,and CIM-554,respectively,in seed cotton yield(SCY) with K application under reduced irrigation conditions.With applied K,the FH-142 showed an increase in net photosynthetic rate by 57.3% as compared with the rest of the cultivars under reduced irrigation over K control.However,the overall performance indicators of K-efficient cultivars like FH-142,CYTO-124,CIM-554,and IUB-2013 were better than BH-212(K in-efficient) under reduced irrigation conditions with applied K at 50 kg·ha^(-1).Fiber quality trait improved significantly with K application under water deficit.The increase in micronaire was 3.6%,4.7%,7.8%,3.4%,and 6.7% in BH-212,IUB-2013,CIM-554,CYTO-124,and FH-142,respectively,with K application at 50 kg·ha^(-1) over without K application under reduced irrigation conditions during the cotton growing season.Similarly,the cultivars FH-142 increased by 12% with K application under reduced irrigation as compared with other cultivars.The performance of K-efficient cultivars under reduced irrigation conditions was 30% better in SCY and quality traits with the application of K at 50 kg·ha^(-1) as compared with K-non-efficient cultivars.Similarly,water use efficiency(WUE)(40.1%) and potassium use efficiency(KUE)(20.2%) were also noted higher in case of FH-142 as compared with other cultivar with K application under reduced conditions.Conclusion Higher expression of GhHAK5aD gene was observed in K-efficient cultivars as compared with K-nonefficient cultivars in roots indicates that GhHAK5aD may be contributing to genotypic differences for K^(+) efficiency in cotton.K-efficient cotton cultivars can be used for the low-K environments and can also be recommended for general cultivars.展开更多
In this paper, we report the results of quality control based in pbysicochemical characteriza- tion and impurities determination of three samples of fluconazole drug substances marketed in Morocco. These samples were ...In this paper, we report the results of quality control based in pbysicochemical characteriza- tion and impurities determination of three samples of fluconazole drug substances marketed in Morocco. These samples were supplied by different pharmaceuticals companies. The sample A, as the discovered product, was supplied by Pfizer, while samples B and C （generics）, were manufactured by two different Indian industries. Solid-state characterization of the three samples was realized with different physicochemical methods as： X-ray powder diffraction, Fourier-transformation infrared spectroscopy, differential scanning calorimetry. High performance liquid chromatography was used to quantify the impurities in the different samples. The results from the physicochemical methods cited above, showed difference in polymorph structure of the three drug substances. Sample A consisted in pure polymorph II1, sample B consisted in pure polymorph I1, sample C consisted in a mixture of fluconazole Form Ili, form II and the monohydrate. This result was confirmed by differential scanning calorimetry. Also it was demonstrated that solvents used during the re-crystallization step were among the origins of these differences in the structure form. On the other hand, the result of the stability study under humidity and temperature showed that fluconazole polymorphic transformation could be owed to the no compliance with the conditions of storage. The HPLC analysis of these compounds showed the presence of specific展开更多
The corrosion inhibition action of three newly synthesized furanylnicotinamidine derivatives namely: 6-[5-{4(dimethylamino)phenyl}furan-2-yl]nicotinamidine(MA-1256), 6-[5-(4-chlorophenyl)furan-2-yl]nicotinamidine(MA-1...The corrosion inhibition action of three newly synthesized furanylnicotinamidine derivatives namely: 6-[5-{4(dimethylamino)phenyl}furan-2-yl]nicotinamidine(MA-1256), 6-[5-(4-chlorophenyl)furan-2-yl]nicotinamidine(MA-1266), and 6-[5-{4-(dimethylamino)phenyl}furan-2-yl]nicotinonitrile(MA-1250) on carbon steel(C-steel) was investigated in 1.0 mol·L-1 HCl solution by weight loss(WL), potentiodynamic polarization(PP), electrochemical impedance spectroscopy(EIS), and electrochemical frequency modulation(EFM)techniques. Morphological analysis was performed on the uninhibited and inhibited C-steel using atomic force microscope(AFM) and Infrared Spectroscopy(ATR-IR) methods. The effect of temperature was studied and discussed. Inspection of experimental results revealed that the inhibition efficiency(IE) increases with the incremental addition of inhibitors and with elevating the temperature of the acid media. The adsorption of furanylnicotinamidine derivatives on C-steel follows Temkin’s isotherm. PP studies indicated that the investigated compounds act as mixed-type inhibitors and showed that p-dimethylaminophenyl furanylnicotinamidine derivative(MA-1256) was the most efficient inhibitor among the other studied derivatives with IE reached(95%)at 21 × 10-6 mol·L-1. MA-1266 is highly soluble in aqueous solution and has non-toxicity profile with LC50 N 37 mg·L-1. Thus, MA-1266 can be a promising green corrosion inhibitor candidate with IE N 91% at 21× 10-6 mol·L-1. The experiments were coupled with computational chemical theories such as quantum chemical and molecular dynamic methods. The experimental results were in good agreement with the computational outputs.展开更多
Honey is being used as a pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics than ever before, so honey testing has become essential to maintain its healthful characteristics and protect pub...Honey is being used as a pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics than ever before, so honey testing has become essential to maintain its healthful characteristics and protect public health. Tetracyclines （TCs） are used for the treatment or prevention of American and European foul broad in bee colonies which are caused especially by two species of bacteria--Paenibacillus larvae and Melissococcus pluton. The present study aimed to determine the tetracycline residues in different types of local and imported honey. The reversed-phase high performance liquid chromatography （HPLC） with ultraviolet detection （UV） was used. Tetracycline and oxytetracycline were extracted from honey with 0.01 M sodium succinate buffer. Of the examined samples, 31.25% and 12.50% had these oxytetracycline and tetracycline residues, respectively. It was observed that all the samples studied have been reported residues of tetracycline and oxytetracycline more than international standards of maximum residue limit （MRLs）.展开更多
This study describes the adsorption behavior of three arylthiophene derivatives namely:2-(4-amidino-3-fluorophenyl)-5-[4-methoxy phenyl] thiophene dihydrochloride salt(MA-1217),2-(4-amidinophenyl)-5-[4-chlorophenyll t...This study describes the adsorption behavior of three arylthiophene derivatives namely:2-(4-amidino-3-fluorophenyl)-5-[4-methoxy phenyl] thiophene dihydrochloride salt(MA-1217),2-(4-amidinophenyl)-5-[4-chlorophenyll thiophene dihydrochloride salt(MA-1316) and 2-(4-amidino-3-fluorophenyl)-5-[4-ch lorophenyllthiophene dihydrochloride salt(MA-1312) at C-steel in 1.0 mol·L^(-1) HCl interface using experimental and theoretical studies.Electrochemical and mass loss measurements showed that the inhibition efficiency(IE) of the arylthiophene derivatives increases with increasing concentrations and exhibited maximum efficiency 89% at 21×10^(-6) mol·L^(-1)(MA-1217) by mass loss method.The investigated arylthiophene derivatives obey the Langmuir adsorption isotherm.From polarization studies the arylthiophene derivatives act as mixed-type inhibitors.Surface analysis were carried out and discussed.The mode of orientation and adsorption of inhibitor molecules on C-steel surface was studied using molecular dynamics(MD) simulations.Quantum chemical parameters as well as the radial distribution function indices and binding energies confirm the experimental results.展开更多
With polyamide(PA)as an efficient sorbent for solid phase extraction(SPE)of Sudan dyes II,III and Red 7B from saffron and urine,their determination by HPLC was performed.The optimum conditions for SPE were achieved us...With polyamide(PA)as an efficient sorbent for solid phase extraction(SPE)of Sudan dyes II,III and Red 7B from saffron and urine,their determination by HPLC was performed.The optimum conditions for SPE were achieved using 7 mL methanol/water(1∶9,v/v,pH 7)as the washing solvent and 3 mL tetrahydrofuran for elution.Good clean-up and high(above 90%)recoveries were observed for all the analytes.The optimized mobile phase composition for HPLC analysis of these compounds was methanol-water(70∶30,v/v).The SPE parameters,such as the maximum loading capacity and breakthrough volume,were also determined for each analyte.The limits of detection(LODs),limits of quantification(LOQs),linear ranges and recoveries for the analytes were 4.6-6.6μg/L,13.0-19.8μg/L,13.0-5 000μg/L(r2>0.99)and 92.5%-113.4%,respectively.The precisions(RSDs)of the overall analytical procedure,estimated by five replicate measurements for Sudan II,III and Red 7B in saffron and urine samples were 2.3%,1.8%and 3.6%,respectively.The developed method is simple and successful in the application to the determination of Sudan dyes in saffron and urine samples with HPLC coupled with UV detection.展开更多
More researches are needed to identify the relevant factors influencing the generation of food waste and devise preventive strategies. The objective of this study is to develop a checklist to assess the good managemen...More researches are needed to identify the relevant factors influencing the generation of food waste and devise preventive strategies. The objective of this study is to develop a checklist to assess the good management practices that have a positive impact on eliminating or minimizing food waste in food service units. A theoretical list of relevant factors impacting the generation of food waste during meal production is developed, taking into account administrative planning, receipt and storage of foods, and the preparation and distribution of meals by food service units. For each factor, several administrative and/or technical actions are identified. To validate the content, this list is submitted to a ten-member expert panel for a final evaluation. The factor (and its respective actions) is considered relevant if 80% of the expert panel members agree on its relevance. All actions employ a dichotomous response of Yes/No. Affirmative responses are expected because these actions are considered to be good management practices that have a positive impact on eliminating or minimizing food waste. Following the panel approval process, a checklist of 12 factors and 102 actions is detailed. The conceptual structure of the checklist for food waste management presented in this study enables a comprehensive understanding of the factors that impact food waste, providing a theoretical basis for future research into the relative importance of the relevant factors and actions identified in this study.展开更多
<b>Background:</b> Shallow wells and boreholes are vital sources of potable water in Hargeisa. This water can be polluted by runoff, in particular during the rainy season, causing outbreaks of waterborne i...<b>Background:</b> Shallow wells and boreholes are vital sources of potable water in Hargeisa. This water can be polluted by runoff, in particular during the rainy season, causing outbreaks of waterborne infections. <b>Objectives:</b> This research aimed at evaluating the microbial quality of shallow wells and boreholes water around Hargeisa, Somaliland. <b>Methods:</b> The total coliform and <i>Escherichia coli</i> count were done by using the membrane filtration method. Overall, 100 ml of each water sample was filtered via a 0.45 μm membrane filter, and then the filters were put on m-Endo agar plates that were incubated at 37°C for 24 to 48 hours. <b>Results:</b> The mean value of total coliform counts for the boreholes and shallow wells ranged from 1.288 × 10<sup>3</sup> to 8.8 × 10<sup>3</sup> CFU/100ml, while the mean value of total <i>E. coli</i> counts also ranged from 3.5 × 10<sup>2</sup> to 4.429 × 10<sup>3</sup> CFU/100ml. Results from this study have demonstrated that all water sources (Arabsiyo, Dararweyne, Darasalaam, Dabaraqas, and Jaleelo) don’t comply with the WHO guideline for drinking water. Results from the analysis of water samples of 28 wells demonstrated a significant correlation between total coliform and <i>E. coli</i> counts (P = 0.01). Therefore, this water is not fit for human consumption unless it is treated. <b>Conclusion:</b> This study has demonstrated that all results of both mean values of total coliform and <i>E. coli</i> counts from groundwater of selected shallow wells and boreholes were beyond WHO standards, so water from Arabsiyo, Jaleelo, Dabaraqas, Dararweyne, and Darasalaam requires treatment before human consumption.展开更多
Water molecules are oriented dipoles joined by hydrogen bonds. When water is heated, this structure collapses (i.e., the entropy increases). When water is re-cooled to a lower temperature, the previous structure is no...Water molecules are oriented dipoles joined by hydrogen bonds. When water is heated, this structure collapses (i.e., the entropy increases). When water is re-cooled to a lower temperature, the previous structure is not re-formed immediately. Sometimes, when the re-cooling is performed within a freezer, there is not enough time for the structure to re-form because of the high cooling rate. The entropy reduction curve as a function of the temperature, S = f(T), shows retardation (a lag) relative to the entropy growth curve. Water that has been heated and re-cooled to the initial temperature shows greater entropy than that before it was heated. This means that, while its molecules now have the same kinetic energy, their thermal motion after heating is less oriented with respect to the structure mentioned above. After re-cooling, random collisions are more likely, owing to this the temperature decreases more quickly.展开更多
The drinking water of Nouakchott,the capital of Mauritania,comes from the Senegal River.The current water treatment is done with aluminum sulfate.In order to monitor the aluminum content,from September 2015 to August ...The drinking water of Nouakchott,the capital of Mauritania,comes from the Senegal River.The current water treatment is done with aluminum sulfate.In order to monitor the aluminum content,from September 2015 to August 2016,three samples were taken monthly for each water source:pretreated water(from Beni Naji treatment station),treated water(from PK17 treatment station)and the water tower.The analysis results of the aluminum content of the treated water(full treatment)and pretreated water(first treatment)showed that the aluminum content of all samples was between 0.03 mg/L and 0.09 mg/L,with an average value of 0.07 mg/L.The World Health Organization stipulates that the normal content of aluminum between 0.1 mg/L and 0.2 mg/l.In the course of the study,the changes of aluminum content in samples from different places were observed.This change can be explained by coagulation-flocculation of aluminum sulfate.The aluminum content of the samples met the World Health Organization standards for drinking water.展开更多
The goal of this topic is a synthesis of the main characteristics of iron in groundwater and the oxidation process used to remove it. Indeed, the kinetics of chemical oxidation of iron (II) was examined with reconstit...The goal of this topic is a synthesis of the main characteristics of iron in groundwater and the oxidation process used to remove it. Indeed, the kinetics of chemical oxidation of iron (II) was examined with reconstituted water (distilled water + iron sulphate) and proceeded to the application in the groundwater samples taken from <em><strong>South Pout</strong></em> (Senegal) precisely in the drilling <strong>PS2</strong>. The sources of iron are natural or anthropogenic. In Senegalese waters, its content is variable and sometimes exceeds the standards of potability. Despite the diversification of iron removal process, chemical oxidation is the most used solution in drinking water treatment plants in Senegalese rural areas. Applied oxidation processes such as aeration and chlorination, however, are insufficient to produce drinking water with an iron concentration in accordance with standards of potability.展开更多
We need to talk about red blood cells.Eukaryotic cells are defined as cells containing distinct nuclei and other membranous organelles.However,red blood cells(RBCs)lack a nucleus and organelles—perhaps to limit the g...We need to talk about red blood cells.Eukaryotic cells are defined as cells containing distinct nuclei and other membranous organelles.However,red blood cells(RBCs)lack a nucleus and organelles—perhaps to limit the generation of reactive oxygen species(Zhang et al.,2011)—and are filled with hemoglobin(Hb).This oxygen carrier is limited to the cytosol by the cell membrane and cytoskeleton,which maintain cellular integrity and deformability as RBCs pass through narrow capillaries(Gratzer,1994)to perform their primary function of gas exchange.展开更多
Antibiotic resistance genes(ARGs)have been detected in various atmospheric environments.Airborne ARGs transmission presents the public health threat.However,it is very difficult to quantify airborne ARGs because of th...Antibiotic resistance genes(ARGs)have been detected in various atmospheric environments.Airborne ARGs transmission presents the public health threat.However,it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of samples.In this study,an optimized protocol for collecting and detecting airborne ARGs was presented.Experimental results showed that recovery efficiency tended to increase initially and then declined over time,and a range of 550-780 copies/mmz of capture loading was recommended to ensure that the recovery efficiency is greater than 75%.As the cell walls were mechanically disrupted and nucleic acids were released,the buffer wash protects ARGs dissolution.Three ratios of buffer volume to membrane area in buffer wash were compared.The highest concentrations of airborne ARGs were detected with 1.4μL/mm^2 buffer wash.Furthermore,the majority of the cells were disrupted by an ultrasonication pretreatment(5 min),allowing the efficiency ARGs detection of airborne samples.While,extending the ultrasonication can disrupt cell structures and gene sequence was broken down into fragments.Therefore,this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different environments.An optimized sampling method was proposed that the buffer wash was 1.4 nL/mm and the ultrasonication duration was 5 min.The indoor airborne ARGs were examined in accordance with the improved protocol in two laboratories.The result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment.展开更多
文摘Artificial fishponds play a pivotal role in global aquaculture, serving as a source of livelihood and nourishment for many communities. Ensuring the sustained health and productivity of Fishes in these environments relies heavily on water quality management. This assessment was done to determine the water quality of ten artificial fishponds in the south-eastern part of Sierra Leone using twelve physicochemical factors (pH, BOD, EC, TDS, turbidity, COD, Fe<sup>2+</sup>, Mg<sup>2+</sup>, Ca<sup>2+</sup>, NH<sub>3</sub>, , and alkalinity) to find out the Water Quality Index (WQI) and spatial distribution of respective parameters. The assessment of artificial fishponds using WQI and Inverse Distant Weighting (IDW) integration represents a relatively underexplored area within the domain of environmental water resources. The WQI was determined using the “Weighted Arithmetic Water Quality Index’’ method. The results of WQI in the study area range from 65.05 to 147.26. Several locations have water quality deemed unsuitable for consumption, while others range from good to very poor. It is essential to address and improve water quality in locations categorized as unsuitable for consumption and very poor to ensure safe and healthy water sources. It was also clear from the calculation that the smaller the mean concentration value of the pH as compared to the ideal value (7), the smaller the WQI value and the better the water quality. To keep the artificial fishpond water in good condition, mass domestic use should be controlled, and draining of surrounding organic matter should be stopped in ponds Bo_001, Kenema_001, and Kenema_002.
文摘Background: Due to their delicious taste, high nutritional content, and health benefits, fruit juices are well-known drinks in many countries and are now an essential component of the modern diet. Objective: Determining the microbiological quality of both packaged and freshly made fruit and milk juices. Method: The spread-plate approach was employed to isolate and count the bacteria. 90 ml of sterile peptone water were blended with 10 ml of well-mixed, packed, and freshly made fruit juices. The samples were sequentially diluted (101 - 105) in accordance with the Indian Manual of Food Microbiological Testing Methods. Results: From eight samples of imported packaged fruit and milk juice, the average of total coliform, staphylococci, and viable bacterial counts were zero, 1.39 × 102, and 2 × 102 CFU/ml, respectively. In contrast, from three samples of locally produced fruit and milk juice, the average of total coliform, staphylococci, and viable bacterial counts were zero, 5.83 × 102, and 2.73 × 103 CFU/ml, respectively. Four samples of handmade prepared fruit and milk juices had a mean of total coliform, staphylococci, and viable bacterial count of 1.441 × 104, 4.1 × 103, and 2.35 × 105 CFU/ml, respectively. Conclusion: 33.3% of the results from microbiological analysis of freshly made fruit and milk juices met the permissible range of the Revised Microbiological Standards for Fruit and Vegetables and Their Products, which were published in 2018 and as well as the Hong Kong Center for Food Safety, whereas 66.7% of the microbiological analyses of freshly prepared fruit and milk juices were above the permissible reference range of GSO standard 2000. 12.5% of the investigated imported and packed fruits and milk juices had one failed test (TSC), which was above the acceptable limit, 87.5% of the tested samples of fruit and milk juices fulfilled the necessary standards of TCC, TVBC, and TSC. 100% of the tested locally manufactured fruit and milk juices complied with TSC, TCC, and TVBC requirements. All investigations showed that freshly made fruit and milk juices were heavily contaminated (Total viable bacterial count, total coliform count, and total staphylococcus count). .
文摘Kalanchoe pinnata (Lam.) Pers. is known as a plant that has many special benefits such as anti-inflammatory and antibacterial. The present study was carried out to perform a phytochemicals study and evaluate the antimicrobial and anti-inflammatory activity of the hydroethanolic extract of Kalanchoe pinnata (Lam.) Pers. leaves. After phytochemicals screening, the content of phenolic compounds, proanthocyanidol and flavonoids in the extract of this plant was determined spectrophotometrically. Antimicrobial activity was assessed using the micro-dilution technique on 96-well plates in liquid medium, combined with agar spreading. Anti-inflammatory activity was assessed using the 1% carrageenan induced rat paw oedema model. Phytochemical screening revealed the presence of alkaloids, saponins, triterpenes and sterols, phenols and flavonoids in the plant extract in varying proportions. The extract contained (0.049 ± 0.03 µg EAG/mg extract) total polyphenols, (0.215 ± 0.025 µg CE/mg extract) proanthocyanidins and (385.435 ± 0.0328 µg ER/mg ES) flavonoids. The hydroethanolic extract of the leaves of this plant inhibited the in vitro growth of the microbial strains studied to varying degrees. The MIC of the extract varied from 12.5 to 25 mg/mL and the BMC from 12.5 to 50 mg/mL. The plant did not show any activity on 1% carrageenan-induced rat paw edema.
文摘This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated December 17, 2001 and n˚407 dated October 3, 2008 respectively. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazolyl-2-yl-2,5-diphenyltetrazolium bromide (MTT) test. No significant toxicity was observed after 14 and 28 days, although a dose-dependent decrease in creatinemia was observed in male rats (the recipe to be used to moderate creatinine levels). Cytotoxicity was without effect on NCM 365 cells. The results obtained justify the use of the recipe in traditional medicine.
基金the Laboratory of Biotechnology and Environment as well as the Food and Nutrition Research Center(CRAN)of Institute for Medical Research and Study of Medicinal Plants in Cameroon for their support
文摘Little is known about the impact of direct sowing under vegetation cover on the production and quality of New Rice for Africa （NERICA） on poor oxisol. In this study, two NERICA varieties （NERICA 3 and NERICA 8） were grown under tropical oxisol soil with very low nutrient contents. Four cultivation systems were used in completely randomized block design, including plowing （control）, unplowed soil with dead vegetation cover （DVC）, unplowed soil with live vegetation cover （LVC） and unplowed soil with mixed vegetation cover （MVC）. DVC significantly improved the exponential growth of NERICAs. NERICA 3 was the more productive （2.16–3.05 t/hm2） compared with NERICA 8 （0.71–1.21 t/hm2）. Cultivation systems improved the nutritional quality of NERICAs. The total protein content of NERICA 3 under DVC and MVC was 84.8% and 75.0% higher than control, respectively. The total soluble carbohydrate contents of NERICA 8 under LVC and MVC was 73.2% and 57.3% higher than control, respectively. These results suggested that conservative approach like direct sowing on unplowed soil with vegetation cover systems can improve the nutritional quality of rainfed NERICAs and their sustainable production under poor oxisol soil in sub-Saharan Africa.
文摘The objective of this study is to assess the quality of the artesian borehole water consumed by the population of the village of Dogbo Ahomey in order to prevent pathologies on the populations. Methodology and Results: The household surveys were supplemented by a campaign to analyze the water samples in the laboratory. The samples were analyzed according to the standardized methods of the American Public Health Association (APHAAWWA-WPCF, 1994). A total of twenty (20) physico-chemical parameters and four (04) microbiological parameters were used to assess the quality of the different water samples. The results of the physico-chemical analysis showed that the water samples studied present values that are below the standards recommended by the WHO and by the Beninese standards for the quality of drinking water (Standards, 2001). From a microbiological analysis point of view, of the four samples studied, two show strong pollution by germs. This could be a significant health risk for households who take water from these sources to meet their needs.
文摘The Brazilian Ministry of Health recommends that by 8 months of age, children should eat the same types of foods consumed by the other members of the family. Thus, this study sought to evaluate whether the nutritional composition of meals specially prepared (SM) for children aged 7 to 18 months in low-income families was superior to that of meals adapted (AM) from the family’s table foods. Protein, fat, carbohydrate, energy, dietary fiber, iron, sodium and sodium chloride values, were determined by chemical analyses and compared to dietetic guidelines. The infants’ hemoglobin levels were also investigated. In total, sixty samples of the infants’ lunch meal (51 AM and 9 SM) were taken for during a home visit. The values of protein, fat, carbohydrate, dietary fiber and energy of the AM were significantly lower, while the sodium and sodium chloride values were significantly higher, compared to those of the SM. The AM also contained significantly more water. No differences were seen with regard to iron values. Sodium chloride amounted for most of the sodium content. Neither the SM nor AM was adequate in terms of iron and sodium. All SM were adequate for protein and fat, whereas AM showed significantly more samples with inadequate energy levels. SM fell within the Acceptable Macronutrient Distribution Range, while AM fell below the lower value for fat and slightly above the upper value for carbohydrate. The prevalence of anemia was 60% in the study population (36/60). In conclusion, meals adapted from the family’s table foods showed a lower nutrient density and a less balanced macronutrient range when compared to meals specially prepared for infants. The main nutritional shortcomings, for both AM and SM, were the extremely low content of iron and the high content of sodium.
文摘Background Under K deficiency the uptake and distribution pattern in plant cells is mediated through different transport proteins and channels which were controlled by specific gene family.Therefore,a hydroponic experiment was conducted under control condition for testing the gene expression pattern of the K transporter under adequate and low K supply levels.After that,a 2-year field experiment was conducted to evaluate five selected cotton cultivars(four K-efficient cultivars,viz.,CIM-554,CYTO-124,FH-142,IUB-2013,and one K non-efficient,BH-212) screened from the initial hydroponics culture experiment and two levels of potassium(0 K_(2)O kg·ha^(-1) and 50 K_(2)O kg·ha^(-1)) were tested under reduced irrigation(50% available water content;50 AWC) and normal irrigation conditions(100% available water content;100 AWC).Result Results revealed that the transcript levels of GhHAK5aD in roots were significantly higher in K^(+) efficient cultivars than that in K^(+) non-efficient cultivars.The GhHAK5aD expression upon K^(+) deficiency was higher in roots but lower in shoots,indicating that GhHAK5aD could have a role in K^(+) uptake in roots,instead of transport of K^(+) from root to shoot.Similarly,under field conditions the cultivar FH-142 showed an increase of 22.3%,4.9%,2.4%,and 1.4% as compared with BH-212,IUB-2013,CYTO-124,and CIM-554,respectively,in seed cotton yield(SCY) with K application under reduced irrigation conditions.With applied K,the FH-142 showed an increase in net photosynthetic rate by 57.3% as compared with the rest of the cultivars under reduced irrigation over K control.However,the overall performance indicators of K-efficient cultivars like FH-142,CYTO-124,CIM-554,and IUB-2013 were better than BH-212(K in-efficient) under reduced irrigation conditions with applied K at 50 kg·ha^(-1).Fiber quality trait improved significantly with K application under water deficit.The increase in micronaire was 3.6%,4.7%,7.8%,3.4%,and 6.7% in BH-212,IUB-2013,CIM-554,CYTO-124,and FH-142,respectively,with K application at 50 kg·ha^(-1) over without K application under reduced irrigation conditions during the cotton growing season.Similarly,the cultivars FH-142 increased by 12% with K application under reduced irrigation as compared with other cultivars.The performance of K-efficient cultivars under reduced irrigation conditions was 30% better in SCY and quality traits with the application of K at 50 kg·ha^(-1) as compared with K-non-efficient cultivars.Similarly,water use efficiency(WUE)(40.1%) and potassium use efficiency(KUE)(20.2%) were also noted higher in case of FH-142 as compared with other cultivar with K application under reduced conditions.Conclusion Higher expression of GhHAK5aD gene was observed in K-efficient cultivars as compared with K-nonefficient cultivars in roots indicates that GhHAK5aD may be contributing to genotypic differences for K^(+) efficiency in cotton.K-efficient cotton cultivars can be used for the low-K environments and can also be recommended for general cultivars.
文摘In this paper, we report the results of quality control based in pbysicochemical characteriza- tion and impurities determination of three samples of fluconazole drug substances marketed in Morocco. These samples were supplied by different pharmaceuticals companies. The sample A, as the discovered product, was supplied by Pfizer, while samples B and C （generics）, were manufactured by two different Indian industries. Solid-state characterization of the three samples was realized with different physicochemical methods as： X-ray powder diffraction, Fourier-transformation infrared spectroscopy, differential scanning calorimetry. High performance liquid chromatography was used to quantify the impurities in the different samples. The results from the physicochemical methods cited above, showed difference in polymorph structure of the three drug substances. Sample A consisted in pure polymorph II1, sample B consisted in pure polymorph I1, sample C consisted in a mixture of fluconazole Form Ili, form II and the monohydrate. This result was confirmed by differential scanning calorimetry. Also it was demonstrated that solvents used during the re-crystallization step were among the origins of these differences in the structure form. On the other hand, the result of the stability study under humidity and temperature showed that fluconazole polymorphic transformation could be owed to the no compliance with the conditions of storage. The HPLC analysis of these compounds showed the presence of specific
文摘The corrosion inhibition action of three newly synthesized furanylnicotinamidine derivatives namely: 6-[5-{4(dimethylamino)phenyl}furan-2-yl]nicotinamidine(MA-1256), 6-[5-(4-chlorophenyl)furan-2-yl]nicotinamidine(MA-1266), and 6-[5-{4-(dimethylamino)phenyl}furan-2-yl]nicotinonitrile(MA-1250) on carbon steel(C-steel) was investigated in 1.0 mol·L-1 HCl solution by weight loss(WL), potentiodynamic polarization(PP), electrochemical impedance spectroscopy(EIS), and electrochemical frequency modulation(EFM)techniques. Morphological analysis was performed on the uninhibited and inhibited C-steel using atomic force microscope(AFM) and Infrared Spectroscopy(ATR-IR) methods. The effect of temperature was studied and discussed. Inspection of experimental results revealed that the inhibition efficiency(IE) increases with the incremental addition of inhibitors and with elevating the temperature of the acid media. The adsorption of furanylnicotinamidine derivatives on C-steel follows Temkin’s isotherm. PP studies indicated that the investigated compounds act as mixed-type inhibitors and showed that p-dimethylaminophenyl furanylnicotinamidine derivative(MA-1256) was the most efficient inhibitor among the other studied derivatives with IE reached(95%)at 21 × 10-6 mol·L-1. MA-1266 is highly soluble in aqueous solution and has non-toxicity profile with LC50 N 37 mg·L-1. Thus, MA-1266 can be a promising green corrosion inhibitor candidate with IE N 91% at 21× 10-6 mol·L-1. The experiments were coupled with computational chemical theories such as quantum chemical and molecular dynamic methods. The experimental results were in good agreement with the computational outputs.
文摘Honey is being used as a pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics than ever before, so honey testing has become essential to maintain its healthful characteristics and protect public health. Tetracyclines （TCs） are used for the treatment or prevention of American and European foul broad in bee colonies which are caused especially by two species of bacteria--Paenibacillus larvae and Melissococcus pluton. The present study aimed to determine the tetracycline residues in different types of local and imported honey. The reversed-phase high performance liquid chromatography （HPLC） with ultraviolet detection （UV） was used. Tetracycline and oxytetracycline were extracted from honey with 0.01 M sodium succinate buffer. Of the examined samples, 31.25% and 12.50% had these oxytetracycline and tetracycline residues, respectively. It was observed that all the samples studied have been reported residues of tetracycline and oxytetracycline more than international standards of maximum residue limit （MRLs）.
基金financial support provided by the Ministry of Higher Education&Scientific Research of Yemen。
文摘This study describes the adsorption behavior of three arylthiophene derivatives namely:2-(4-amidino-3-fluorophenyl)-5-[4-methoxy phenyl] thiophene dihydrochloride salt(MA-1217),2-(4-amidinophenyl)-5-[4-chlorophenyll thiophene dihydrochloride salt(MA-1316) and 2-(4-amidino-3-fluorophenyl)-5-[4-ch lorophenyllthiophene dihydrochloride salt(MA-1312) at C-steel in 1.0 mol·L^(-1) HCl interface using experimental and theoretical studies.Electrochemical and mass loss measurements showed that the inhibition efficiency(IE) of the arylthiophene derivatives increases with increasing concentrations and exhibited maximum efficiency 89% at 21×10^(-6) mol·L^(-1)(MA-1217) by mass loss method.The investigated arylthiophene derivatives obey the Langmuir adsorption isotherm.From polarization studies the arylthiophene derivatives act as mixed-type inhibitors.Surface analysis were carried out and discussed.The mode of orientation and adsorption of inhibitor molecules on C-steel surface was studied using molecular dynamics(MD) simulations.Quantum chemical parameters as well as the radial distribution function indices and binding energies confirm the experimental results.
基金the financial support of Institute of Standards and Industrial Research of Iran(Khorasan-e-Razavi Province)
文摘With polyamide(PA)as an efficient sorbent for solid phase extraction(SPE)of Sudan dyes II,III and Red 7B from saffron and urine,their determination by HPLC was performed.The optimum conditions for SPE were achieved using 7 mL methanol/water(1∶9,v/v,pH 7)as the washing solvent and 3 mL tetrahydrofuran for elution.Good clean-up and high(above 90%)recoveries were observed for all the analytes.The optimized mobile phase composition for HPLC analysis of these compounds was methanol-water(70∶30,v/v).The SPE parameters,such as the maximum loading capacity and breakthrough volume,were also determined for each analyte.The limits of detection(LODs),limits of quantification(LOQs),linear ranges and recoveries for the analytes were 4.6-6.6μg/L,13.0-19.8μg/L,13.0-5 000μg/L(r2>0.99)and 92.5%-113.4%,respectively.The precisions(RSDs)of the overall analytical procedure,estimated by five replicate measurements for Sudan II,III and Red 7B in saffron and urine samples were 2.3%,1.8%and 3.6%,respectively.The developed method is simple and successful in the application to the determination of Sudan dyes in saffron and urine samples with HPLC coupled with UV detection.
文摘More researches are needed to identify the relevant factors influencing the generation of food waste and devise preventive strategies. The objective of this study is to develop a checklist to assess the good management practices that have a positive impact on eliminating or minimizing food waste in food service units. A theoretical list of relevant factors impacting the generation of food waste during meal production is developed, taking into account administrative planning, receipt and storage of foods, and the preparation and distribution of meals by food service units. For each factor, several administrative and/or technical actions are identified. To validate the content, this list is submitted to a ten-member expert panel for a final evaluation. The factor (and its respective actions) is considered relevant if 80% of the expert panel members agree on its relevance. All actions employ a dichotomous response of Yes/No. Affirmative responses are expected because these actions are considered to be good management practices that have a positive impact on eliminating or minimizing food waste. Following the panel approval process, a checklist of 12 factors and 102 actions is detailed. The conceptual structure of the checklist for food waste management presented in this study enables a comprehensive understanding of the factors that impact food waste, providing a theoretical basis for future research into the relative importance of the relevant factors and actions identified in this study.
文摘<b>Background:</b> Shallow wells and boreholes are vital sources of potable water in Hargeisa. This water can be polluted by runoff, in particular during the rainy season, causing outbreaks of waterborne infections. <b>Objectives:</b> This research aimed at evaluating the microbial quality of shallow wells and boreholes water around Hargeisa, Somaliland. <b>Methods:</b> The total coliform and <i>Escherichia coli</i> count were done by using the membrane filtration method. Overall, 100 ml of each water sample was filtered via a 0.45 μm membrane filter, and then the filters were put on m-Endo agar plates that were incubated at 37°C for 24 to 48 hours. <b>Results:</b> The mean value of total coliform counts for the boreholes and shallow wells ranged from 1.288 × 10<sup>3</sup> to 8.8 × 10<sup>3</sup> CFU/100ml, while the mean value of total <i>E. coli</i> counts also ranged from 3.5 × 10<sup>2</sup> to 4.429 × 10<sup>3</sup> CFU/100ml. Results from this study have demonstrated that all water sources (Arabsiyo, Dararweyne, Darasalaam, Dabaraqas, and Jaleelo) don’t comply with the WHO guideline for drinking water. Results from the analysis of water samples of 28 wells demonstrated a significant correlation between total coliform and <i>E. coli</i> counts (P = 0.01). Therefore, this water is not fit for human consumption unless it is treated. <b>Conclusion:</b> This study has demonstrated that all results of both mean values of total coliform and <i>E. coli</i> counts from groundwater of selected shallow wells and boreholes were beyond WHO standards, so water from Arabsiyo, Jaleelo, Dabaraqas, Dararweyne, and Darasalaam requires treatment before human consumption.
文摘Water molecules are oriented dipoles joined by hydrogen bonds. When water is heated, this structure collapses (i.e., the entropy increases). When water is re-cooled to a lower temperature, the previous structure is not re-formed immediately. Sometimes, when the re-cooling is performed within a freezer, there is not enough time for the structure to re-form because of the high cooling rate. The entropy reduction curve as a function of the temperature, S = f(T), shows retardation (a lag) relative to the entropy growth curve. Water that has been heated and re-cooled to the initial temperature shows greater entropy than that before it was heated. This means that, while its molecules now have the same kinetic energy, their thermal motion after heating is less oriented with respect to the structure mentioned above. After re-cooling, random collisions are more likely, owing to this the temperature decreases more quickly.
文摘The drinking water of Nouakchott,the capital of Mauritania,comes from the Senegal River.The current water treatment is done with aluminum sulfate.In order to monitor the aluminum content,from September 2015 to August 2016,three samples were taken monthly for each water source:pretreated water(from Beni Naji treatment station),treated water(from PK17 treatment station)and the water tower.The analysis results of the aluminum content of the treated water(full treatment)and pretreated water(first treatment)showed that the aluminum content of all samples was between 0.03 mg/L and 0.09 mg/L,with an average value of 0.07 mg/L.The World Health Organization stipulates that the normal content of aluminum between 0.1 mg/L and 0.2 mg/l.In the course of the study,the changes of aluminum content in samples from different places were observed.This change can be explained by coagulation-flocculation of aluminum sulfate.The aluminum content of the samples met the World Health Organization standards for drinking water.
文摘The goal of this topic is a synthesis of the main characteristics of iron in groundwater and the oxidation process used to remove it. Indeed, the kinetics of chemical oxidation of iron (II) was examined with reconstituted water (distilled water + iron sulphate) and proceeded to the application in the groundwater samples taken from <em><strong>South Pout</strong></em> (Senegal) precisely in the drilling <strong>PS2</strong>. The sources of iron are natural or anthropogenic. In Senegalese waters, its content is variable and sometimes exceeds the standards of potability. Despite the diversification of iron removal process, chemical oxidation is the most used solution in drinking water treatment plants in Senegalese rural areas. Applied oxidation processes such as aeration and chlorination, however, are insufficient to produce drinking water with an iron concentration in accordance with standards of potability.
基金A.T.A.was supported by an EHA Research Grant award granted by the European Hematology Association(RG202212-03039)A.D.was supported by funds by the National Heart,Lung,and Blood Institute(R21HL150032,R01HL146442,R01HL149714,R01HL161004)S.L.S and A.D.were supported by the National Institutes of Health(NIH R01-HL148151).
文摘We need to talk about red blood cells.Eukaryotic cells are defined as cells containing distinct nuclei and other membranous organelles.However,red blood cells(RBCs)lack a nucleus and organelles—perhaps to limit the generation of reactive oxygen species(Zhang et al.,2011)—and are filled with hemoglobin(Hb).This oxygen carrier is limited to the cytosol by the cell membrane and cytoskeleton,which maintain cellular integrity and deformability as RBCs pass through narrow capillaries(Gratzer,1994)to perform their primary function of gas exchange.
基金This study was supported by the National Natural Science Foundation of China(Grant No.51678402)the key technologies R&D program of Tianjin(No.20ZXGBSY00100).
文摘Antibiotic resistance genes(ARGs)have been detected in various atmospheric environments.Airborne ARGs transmission presents the public health threat.However,it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of samples.In this study,an optimized protocol for collecting and detecting airborne ARGs was presented.Experimental results showed that recovery efficiency tended to increase initially and then declined over time,and a range of 550-780 copies/mmz of capture loading was recommended to ensure that the recovery efficiency is greater than 75%.As the cell walls were mechanically disrupted and nucleic acids were released,the buffer wash protects ARGs dissolution.Three ratios of buffer volume to membrane area in buffer wash were compared.The highest concentrations of airborne ARGs were detected with 1.4μL/mm^2 buffer wash.Furthermore,the majority of the cells were disrupted by an ultrasonication pretreatment(5 min),allowing the efficiency ARGs detection of airborne samples.While,extending the ultrasonication can disrupt cell structures and gene sequence was broken down into fragments.Therefore,this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different environments.An optimized sampling method was proposed that the buffer wash was 1.4 nL/mm and the ultrasonication duration was 5 min.The indoor airborne ARGs were examined in accordance with the improved protocol in two laboratories.The result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment.
