Drought is one of the critical conditions for the growth and productivity of many crops including mung bean(Vigna radiata L.Wilczek).Screening of genotypes for variations is one of the suitable strategies for evaluati...Drought is one of the critical conditions for the growth and productivity of many crops including mung bean(Vigna radiata L.Wilczek).Screening of genotypes for variations is one of the suitable strategies for evaluating crop adaptability and global food security.In this context,the study investigated the physiological and biochemical responses of four drought tolerant(BARI Mung-8,BMX-08010-2,BMX-010015,BMX-08009-7),and four drought sensitive(BARI Mung-1,BARI Mung-3,BU Mung-4,BMX-05001)mung bean genotypes under wellwatered(WW)and water deficit(WD)conditions.The WW treatment maintained sufficient soil moisture(22%±0.5%,i.e.,30%deficit of available water)by regularly supplying water.Whereas,the WD treatment was maintained throughout the growing period,and water was applied when the wilting symptom appeared.The drought tolerant(DT)genotypes BARI Mung-8,BMX-08010-2,BMX-010015,BMX-08009-7 showed a high level of proline accumulation(2.52–5.99 mg g^(−1) FW),photosynthetic pigment(total chlorophyll 2.96–3.27 mg g^(−1) FW at flowering stage,and 1.62–2.38 mg g^(−1) FW at pod developing stage),plant water relation attributes including relative water content(RWC)(82%–84%),water retention capacity(WRC)(12–14)as well as lower water saturation deficit(WSD)(19%–23%),and water uptake capacity(WUC)(2.58–2.89)under WD condition,which provided consequently higher relative seed yield.These indicate that the tolerant genotypes gained better physiobiochemical attributes and adaptability in response to drought conditions.Furthermore,the genotype BMX-08010-2 showed superiority in terms of those physio-biochemical traits,susceptibility index(SSI)and stress tolerance index(STI)to other genotypes.Based on the physiological and biochemical responses,the BMX-08010-2 was found to be a suitable genotype for sustaining yield under drought stress,and subsequently,it could be recommended for crop improvement through hybridization programs.In addition,the identified traits can be used as markers to identify tolerant genotypes for drought-prone areas.展开更多
Ladakh’s cropped and non-cropped areas suffer greatly from weedy invasion under subsistence agricultural system where weeding is not a priority but total crop + weed biomass together contribute to livestock feeding s...Ladakh’s cropped and non-cropped areas suffer greatly from weedy invasion under subsistence agricultural system where weeding is not a priority but total crop + weed biomass together contribute to livestock feeding significantly. As agriculture along with livestock rearing is major activity of livelihood options, thereby contributing significantly to Ladakh economy and income generation for supporting resource poor farmers. Amongst various invasive weed species, the common reed (Phragmites australis) has become a serious ecological threat for agri-silvi-pastoral system biodiversity and accelerated its expansion in cropped lands including many wetland and salt-affected habitats of cold arid region due to its intermediate plant growth habit of C3 - C4 photosynthetic ecotypes. This character makes Phragmites a climate-resilient species, which is a major challenge for cold arid agriculture in the changing climatic patterns. Simultaneously, it is one of the best fodder substitute available under harsh climate. This paper deals with its unique characters and potential threats to the agrobiodiversity of Ladakh.展开更多
Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity.Therefore,molecular characterization of germplasms is important to ob...Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity.Therefore,molecular characterization of germplasms is important to obtain target cultivars with desirable traits.Sweet potato[Ipomoea batatas(L.)Lam]is widely considered the world’s most important crop,with great diversity in morphological and phenotypic traits.The genetic diversity of 20 sweet potato germplasms originating from Bangladesh,CIP,Philippines,Taiwan,and Malaysia were compared,which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization.This information was effective in differentiating or clustering the sweet potato genotypes.A total of 64 alleles were generated using the 20 primers throughout the 20 germplasm samples,with locus IBS97 having the highest number of alleles(5),whereas locus IbU33 had the fewest alleles(2).The alleles varied in size from 105(IbU31)to 213 base pairs(IBS34).The Polymorphism Information Content(PIC)values for the loci IbL46 and IBS97 varied from 0.445 to 0.730.IBS97 has the highest number of effective alleles(3.704),compared to an average of 2.520.The average Shannon’s diversity index(H)was 1.003,ranging from 0.673 in IbU3 to 1.432 in IBS97.The value of gene flow(Nm)varied between 0.000 and 0.005,with an average of 0.003,whereas genetic differentiation(FST-values)ranged between 0.901 and 1.000.The sweet potato germplasm included in this study had a broad genetic base.SP1 vs.SP9 and SP12 vs.SP18 germplasm pairings had the greatest genetic distance(GD=0.965),while SP1 vs.SP2 germplasm couples had the least genetic diversity(GD=0.093).Twenty genotypes were classified into two groups in the UPGMA dendrogram,with 16 genotypes classified as group“A”and the remaining four genotypes,SP10,SP18,SP19,and SP20,classified as group“B.”According to cluster analysis,the anticipated heterozygosity(gene diversity)of Nei(1973)was 0.591 on average.In summary,SSR markers successfully evaluated the genetic relationships among the sweet potato accessions used and generated a high level of polymorphism.The results of the present study will be useful for the management of germplasm,improvement of the current breeding strategies,and the release of new cultivars as varieties.展开更多
We evaluated the growth and crown traits of 36 poplar clones at two distinct agro-climatic regions of Punjab (Ludhiana and Bathinda) in northwestern India, following randomized block design with three replications and...We evaluated the growth and crown traits of 36 poplar clones at two distinct agro-climatic regions of Punjab (Ludhiana and Bathinda) in northwestern India, following randomized block design with three replications and plot size of four trees. Significant differences among clones (p<0.001) were observed for diameter at breast height (DBH), tree height, volume, crown width and number of branches under both the site conditions. Clones 'G-3', '25-N' and '41-N' at Ludhiana and 'G-3', 'RD-01' and 'S 7 C 8 ' at Bathinda were found to be superior for volume production. All growth and crown traits registered significantly higher values at Ludhiana in comparison to those at Bathinda. Clone × site interaction was also significant (p<0.001). For volume, clones 'L-62/84', '113520', '25-N' and 'S 4 C 2 ' witnessed huge fluctuations in ranking between sites. The correlations between growth traits were positive and highly significant (p<0.001) at both sites. The clonal mean heritability was moderate for DBH and volume both at Ludhiana (0.61 0.66) and Bathinda (0.61 0.62). Across sites, the genetic advance was the highest for volume (49.76%) and the lowest (6.50%) in case of height.展开更多
An attempt was made to observe the effect of pre-harvest bagging with spun-bound fabric bags on color and quality of Delicious apple. Bagging was done about a month before harvesting and removed 3-day before harvestin...An attempt was made to observe the effect of pre-harvest bagging with spun-bound fabric bags on color and quality of Delicious apple. Bagging was done about a month before harvesting and removed 3-day before harvesting. Bagged and non-bagged fruits were stored at 2℃ ± 1℃ and 90%-95% RH. Observations were recorded on color and fruit quality attributes such as total phenolics, AOX activity, fruit Ca contents, LOX activity, SSC and ascorbic acid contents at harvest and during storage. Our studies have revealed that bagged fruits have better color development (Hunter “a” = 52) than non-bagged fruits at harvest (Hunter “a” = 38), which declined slightly during storage. Similarly, at harvest, bagged fruits contained high amounts of Ca (5.38 mg/100g) and total phenolics (9.3 mg GAE/100gpulp) exhibited higher AOX activity (12.6 μmoles Trolox g-1), and had better SSC and ascorbic acid contents than non-bagged fruits, and there was a decline in all recorded parameters during storage. Bagged fruits exhibited lower LOX activity (1.38 μmoles min-1 g-1 FW) at harvest than non-bagged fruits (2.14 μmoles min-1 g-1 FW), indicating that non-bagged fruits were more senescent than bagged fruits. Further, LOX activity increased during storage both in bagged and non-bagged apples but increase in LOX activity was slower in bagged apples than in non-bagged apples.展开更多
Objective:To evaluate the insecticidal,antimicrobial and antioxidant activities of bulb extracts of Allium sativum(A.sativum).Methods:Dried bulbs of A.sativum were extracted with different solvents and evaluated for i...Objective:To evaluate the insecticidal,antimicrobial and antioxidant activities of bulb extracts of Allium sativum(A.sativum).Methods:Dried bulbs of A.sativum were extracted with different solvents and evaluated for insecticidal,antimicrobial and antioxidant activities.Results: Aqueous and methanol extracts showed highest insecticidal activity(mortality rate of 81%and 64%respectively) against the larvae of Spodoptera litura(S.litura) at a concentration of 1000 ppm.With regard to antimicrobial activity,aqueous extract exhibited antibacterial activity against gram positive(Bacillus subtilis,Staphylococcus aureu,) and gram negative(Escherichia coli and Klebsiella pneumonia) strains and antifungal activity against Candida albicans.While methanol extract showed antimicrobial activity against all the tested micro organisms except two(Staphylococcus aureus and Candida albicans),the extracts of hexane,chloroform and ethyl acetate did not show any anti microbial activity.Minimum inhibitory concentration of aqueous and methanol extracts against tested bacterial and fungal strains was 100-150μg/mL. Antioxidant activity of the bulb extracts was evaluated in terms of inhibition of free radicals by 2.2’-diphenly-l-picrylhydrazyl.Aqueous and methanol extracts exhibited strong antioxidant activity(80%-90%of the standard).Conclusions:Antioxidant and antimicrobial activity of A.sativum against the tested organisms therefore,provides scientific basis for its utilization in traditional and folk medicine.Also,our results demonstrated the insecticidal efficacy of A. sativum against S.litura,a polyphagous insect.展开更多
Objective:To formulate a simple rapid procedure for bioreduction of silver nanoparticles using aqueous leaves extract of Moringa oleifera(M.oleifera).Methods:10 mL of leaf extract was mixed to 90 mL of 1 mM aqueous of...Objective:To formulate a simple rapid procedure for bioreduction of silver nanoparticles using aqueous leaves extract of Moringa oleifera(M.oleifera).Methods:10 mL of leaf extract was mixed to 90 mL of 1 mM aqueous of AgNO_3 and was heated at 60-80 ℃ for 20 min.A change from brown to reddish color was observed.Characterization using UV-Vis spectrophotometry, Transmission Electron Microscopy(TEM) was performed.Results:TEM showed the formation of silver nanoparticles with an average size of 57 nm.Conclusions:M.oleifera demonstrates strong potential for synthesis of silver nanoparticles by rapid reduction of silver ions(Ag^+ to Ag^0). Biological methods are good competents for the chemical procedures,which are eco-friendly and convenient.展开更多
The presence of Al in the rhizosphere of rice in acid soil restricts root growth and significantly reduces crop productivity.In this study,the effects of Al(30,60 and 90μg/mL)on seedling root growth,number of primary...The presence of Al in the rhizosphere of rice in acid soil restricts root growth and significantly reduces crop productivity.In this study,the effects of Al(30,60 and 90μg/mL)on seedling root growth,number of primary roots per seedling,seedling shoot length,number of leaves per seedling,seedling fresh weight,and seedling dry weight were studied.Rice genotypes were classified into three different classes,namely,tolerant,moderately tolerant,and susceptible,based on root tolerance index.The method of hydroponic culture was modified,and elaborated in the text.Toxic levels of Al in nutrient solution significantly decreased seedling root growth,number of primary roots,seedling shoot length,number of leaves per seedling,seedling fresh weight,and seedling dry weight.Few genotypes showed longer root length at 30μg/mL Al in nutrient solutions compared with the control.High levels of Al in nutrient solutions were highly toxic for rice seedlings.Based on root tolerance index,Radhunipagal,Gobindobhog,Badshabhog,Kalobhog,UBKVR-11,UBKVR-16,UBKVR-18,Khasha and IVT4007-B were classified as tolerant genotypes,and these genotypes may be used as donors for breeding of Altoxicity tolerance.展开更多
A long-term field study was initiated during 1995 at Central Soil Salinity Research Institute, Regional Research Station, Lucknow(26°47′58′′ N and 80°46′24′′ E) to analyze the effect of agroforestry sy...A long-term field study was initiated during 1995 at Central Soil Salinity Research Institute, Regional Research Station, Lucknow(26°47′58′′ N and 80°46′24′′ E) to analyze the effect of agroforestry systems on amelioration of alkali soils. Three agroforestry systems(pastoral, silvipastoral and silvicultural) were compared with the control where no agroforestry system was introduced. Tree-based silvicultural and silvipastoral systems were characterized by tree species Prosopis juliflora and Acacia nilotica along with grass species Leptochloa fusca, Panicum maximum, Trifolium alexandrium and Chloris gayana. Growth of ten-year-old Prosopis juliflora and Acacia nilotica planted in combination with grasses was significantly higher over the silviculture system with the same species. Tree biomass yields of P. juliflora(77.20 t?ha-1) and A. nilotica(63.20 t?ha-1) planted under silvipastoral system were significantly higher than the sole plantation of(64.50 t?ha-1 and 52.75 t?ha-1). Fodder yield under the pastoral system was significantly higher than the silvipastoral system during initial years but it was at par with that of silvipastoral systems after eight years of plantation. The microbial biomass carbon in the soils of silvipastoral systems was significantly higher than in soils under sole plantation of trees and control systems. The Prosopis-based silvipastoral system proved more effective in reducing soil pH, displacing Na+ from the exchange complex, increasing organic carbon and available N, P and K. Improvement in soil physical properties such as bulk density, porosity, soil moisture and infiltration rate was higher in the Prosopis-based silvipastoral system than in the silviculture system or control. On the basis of biomass production and improvement in soil health due to tree + grass systems, silvipastoral agroforestry system could be adopted for sustainable reclamation of highly alkali soils.展开更多
Joha rice of Assam is a class of rice with aroma, good cooking qualities and excellent palatability, which are grown in different places of Assam with their adaptability to local situations. A total of 143 simple sequ...Joha rice of Assam is a class of rice with aroma, good cooking qualities and excellent palatability, which are grown in different places of Assam with their adaptability to local situations. A total of 143 simple sequence repeat markers were used in this study to reveal sufficient genetic variations among the 40 Joha and 14 non-Joha rice genotypes. Polymorphism information content of these markers were from 0.17 to 0.88, where the average of 3.7 alleles were observed. The model-based population assignment and dendrogram analysis identified three distinct groups in the 54 rice accessions, which established a clear identity of Joha accessions from the Basmati accessions, indicating high diversity and strong population structure in Joha rice. Furthermore, a total of 29 significant marker-trait associations(P < 0.05) for 10 characters were detected. The QTLs related with yield and grain quality can be used effectively in crop improvement programs and for further fine mapping and validation of specific genes to develop gene-based perfect markers in rice breeding and for mining of better alleles of these genes in Joha rice collections.展开更多
Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important food legume of the semi-arid tropics (SAT) sustaining livelihood of millions of people. Stagnant and unstable yield per hectare all over the world is the chara...Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important food legume of the semi-arid tropics (SAT) sustaining livelihood of millions of people. Stagnant and unstable yield per hectare all over the world is the characteristic feature of this crop. This is primarily ascribed to its susceptibility/sensitivity to a number of biotic and abiotic factors. Among biotic factors, insects such as pod borer (Helicoverpa armigera), pod fly (Melanoagromyza obtusa) and spotted borer (Maruca vitrata) substantially damage the crop and result in significant economic losses. Management of these insects by genetic means has always been considered environment friendly approach. However, genetic improvement has always been impeded by limited genetic variability in the primary gene pool of pigeonpea. Wild species present in the secondary and tertiary gene pools have been reported to carry resistance for such insects. However, transfer of resistance through conventional backcrossing has not been much successful. It calls for gene introgression through marker assisted backcrossing (MABC) or advanced backcross breeding (AB breeding). In this review, we have attempted to assess the progress made through conventional and molecular breeding and suggested the ways to move further towards genetic enhancement for insects resistance in展开更多
Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking quality.However,the yield of Varalu is substantially low since it is being affected by reproducti...Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking quality.However,the yield of Varalu is substantially low since it is being affected by reproductive drought stress along with the blast disease.The genetic improvement of Varalu was done by introgressing a major yield QTL,qDTY_(12.1),along with two major blast resistance genes i.e.Pi54 and Pi1 through marker-assisted backcross breeding.Both traits were transferred till BC_(2) generation and intercrossing was followed to pyramid the two traits.Stringent foreground selection was carried out using linked markers as well as peak markers(RM28099,RM28130,RM511 and RM28163)for the targeted QTL(qDTY_(12.1)),RM206 for Pi54 and RM224 for Pi1.Extensive background selection was done using genome-wide SSR markers.Six best lines(MSM-36,MSM-49,MSM-53,MSM-57,MSM-60 and MSM-63)having qDTY_(12.1) and two blast resistance genes in homozygous condition with recurrent parent genome of 95.0%-96.5% having minimal linkage drag of about 0.1 to 0.7 Mb were identified.These lines showed yield advantage under drought stress as well as irrigated conditions.MSM-36 showed better performance in the national coordinated trials conducted across India,which indicated that improved lines of Varalu expected to replace Varalu and may have an important role in sustaining rice production.The present study demonstrated the successful marker-assisted pyramiding strategy for introgression of genes/QTLs conferring biotic stress resistance and yield under abiotic stress in rice.展开更多
The common practice of establishing rice in the rice-wheat system in India is manual transplanting of seedlings in the puddled soil. Besides being costly, cumbersome, and time consuming, puddling results in degradatio...The common practice of establishing rice in the rice-wheat system in India is manual transplanting of seedlings in the puddled soil. Besides being costly, cumbersome, and time consuming, puddling results in degradation of soil and the formation of a hard pan, which impedes root growth of subsequent upland crops. In addition, decreased availability and increasing cost of labor have increased the cost of rice cultivation through conventional methods. Because of these concerns, there is a need for mechanized transplanting of rice which is less labor-intensive and can ensure optimum plant population under nonpuddled and/or no-till conditions. A large number of on-farm trials were conducted at farmers’ fields in Haryana, India, from 2006 to 2010 to evaluate the performance of the mechanical transplanted rice (MTR) under nonpuddled and no-till situations as compared to conventional puddled transplant rice (CPTR). Compared with CPTR, nonpuddled MTR produced 3%-11% higher grain yield in different years. Rice cultivars, viz. HKR47, HKR127, PR113, PR114, PB1, PB1121, CSR30, and Arize6129, performed consistently better under nonpuddled MTR as compared to CPTR. Performance of different cultivars (PR113, PR114, HKR47, and Pusa 44) was also better under no-till MTR as compared to CPTR. The “basmati” cultivar CSR30 performed equally in no-till MTR and CPTR systems. The results of our study suggest that rice can be easily grown under nonpuddled and no-till conditions with yield advantages over the CPTR system. Even in the case of similar yield between CPTR and MTR systems, the MTR system will help in reducing labor requirement and ultimately, will increase overall profits to farmers.展开更多
The objectives of the present investigations are to see the effects of biomass power plant effluent on the carbohydrates, aminoacids, nitrite and nitrite enzyme activitiesand proline of Arachis hypogaea L. var TCGS 32...The objectives of the present investigations are to see the effects of biomass power plant effluent on the carbohydrates, aminoacids, nitrite and nitrite enzyme activitiesand proline of Arachis hypogaea L. var TCGS 320 under controlled pot culture methods. Plants were cultivated with 25, 50, 75 and 100% of the effluent and a control without the effluent. The treatment of the crop with 25% of the effluent has shown stimulatory effect on all the biochemical parameters studied. Carbohydrates, starch, aminoacids, protein, nitrate and nitrite reductase enzymatic activities have increased in 10, 15, 20 DAS (days after sowing). In 25 and 30 DAS all biochemical parameters have decreased, due to environmental factors (rainfall, temperature, humidity, etc.). The present investigation clearly indicated that the biomass power plant effluent has stimulatory effect on all the biochemical contents at lower concentration, and at higher concentration they have deleterious effects.展开更多
Sugarcane is one of the major important sugar yielding crops in Bangladesh.As an exhaustive crop,sugarcane removes a huge amount of plant nutrients from the soil.However,the combined use of organic and inorganic ferti...Sugarcane is one of the major important sugar yielding crops in Bangladesh.As an exhaustive crop,sugarcane removes a huge amount of plant nutrients from the soil.However,the combined use of organic and inorganic fertilizers can be a good approach to deal with nutrient depletion and promote sustainable crop production as well as improve soil health.Therefore,an attempt was made to identify the most fruitful and profitable integrated nutrient management on the aspects of growth,yield and quality of sugarcane in two consecutive growing seasons.Seven treatments:T_(1)=Control,T_(2)=165:55:120:30:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1),T_(3)=Poultry Litter(PL)at 5 t ha^(−1)+95:51:87:9:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1),T_(4)=Cow Dung(CD)at 15 t ha^(−1)+36:52:60:17:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1),T_(5)=Press Mud(PM)at 15 t ha^(−1)+10:50:43:0:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1),T_(6)=Mustard Oil Cake(MOC)at 0.5 t ha^(−1)+140:54:115:25:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1)and T_(7)=GM(Green Manure)at 5 t ha^(−1)+140:53:100:28:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1)were used in this experiment.Two years data showed that treatment T_(3)produced the maximum amount of tillers,total dry matter yield,millable sugarcane,cane yield and sugar yield,followed by the T_(4)treatment.The highest stalk heights were recorded in the T_(3)treatment,which was statistically similar to all other treatments except T_(1)and T_(2).The juice quality parameters viz.,brix and pol in cane were found significant in treatment T_(3)while the highest purity was obtained in the T_(7)treatment.All the data of Jaggery(goor)quality parameters,the highest sucrose content,color transmittance,Jaggery(goor)recovery and the lowest ash content of Jaggery(goor)were observed in the T_(3)treatment,which was statistically similar to the T_(4)treatment in both seasons.The highest cost of production was obtained from the T_(6)treatment while the highest gross return,net return and BCR were recorded in the T_(3)treatment.No significant changes were found in one cycle of sugarcane in initial and post-harvest soil characteristics viz.,pH,organic carbon,total N,and available P,K and S contents due to integrated use of different fertilizer packages.From the experimental findings,it was concluded that treatment T_(3)followed by T_(4)treatment would be the better productive and profitable integrated nutrient management technology for ensuring higher yields and quality of sugarcane without soil fertility degradation in the High Ganges River Floodplain soils.展开更多
Swertia chirayita is a highly endangered and very well known medicinal plant enlisted in the IUCN list of endangered plants in the world. The plant is currently having a very low population in eastern Himalayan region...Swertia chirayita is a highly endangered and very well known medicinal plant enlisted in the IUCN list of endangered plants in the world. The plant is currently having a very low population in eastern Himalayan region due to its very low germinability in its own natural habitat. The plant population was found to have a range of diversity in morphological observations and some of the morphovariants were found to be very low among the variant populations which created a concern due to its diminishing range of genetic base in this region. A basic group of 5 morphovariants and 24 sub variants were identified and separately maintained. It is a pluriannual plant and reproductive or flowering stage was observed after completion of two years of vegetative stage from the time of seed germination. A range of morphovariant diversity was observed in the populations of Neora Valley and Lava region of eastern Himalaya in our investigation. Diversity was critically observed in some of the parameters of different morphovariants in the population. Conservation practices were generally done by not making groups or sub groups of this endangered plant while we proposed the way of conservation by making groups for proper conservation of this vulnerable plant.展开更多
Genome-wide association study(GWAS)was performed for 16 agronomic traits including nitrogen use efficiency(NUE)and yield-related components using a panel of 190 mainly japonica rice varieties and a set of 38390 single...Genome-wide association study(GWAS)was performed for 16 agronomic traits including nitrogen use efficiency(NUE)and yield-related components using a panel of 190 mainly japonica rice varieties and a set of 38390 single nucleotide polymorphism(SNP)markers.This panel was evaluated under rainfed upland conditions in Madagascar in two consecutive cropping seasons with two contrasted nitrogen input levels.Using another set of five grain traits,we identified previously known genes(GW5,GS3,Awn1 and Glabrous1),thus validating the pertinence and accuracy of our datasets for GWAS.A total of 369 significant associations were detected between SNPs and agronomic traits,gathered into 46 distinct haplotype groups and 28 isolated markers.Few association signals were identified for the complex quantitative trait NUE,however,larger number of quantitative trait loci(QTLs)were detected for its component traits,with 10 and 2 association signals for nitrogen utilization efficiency and nitrogen uptake efficiency,respectively.Several detected association signals co-localized with genes involved in nitrogen transport or nitrogen remobilization within 100 kb.The present study thus confirmed the potential of GWAS to identify candidate genes and new loci associated with agronomic traits.However,because of the quantitative and complex nature of NUE-related traits,GWAS might have not captured a large number of QTLs with limited effects.展开更多
基金The authors extend their appreciation to the researchers Supporting Project No.(RSP-2021/298),King Saud University,Riyadh,Saudi Arabia.
文摘Drought is one of the critical conditions for the growth and productivity of many crops including mung bean(Vigna radiata L.Wilczek).Screening of genotypes for variations is one of the suitable strategies for evaluating crop adaptability and global food security.In this context,the study investigated the physiological and biochemical responses of four drought tolerant(BARI Mung-8,BMX-08010-2,BMX-010015,BMX-08009-7),and four drought sensitive(BARI Mung-1,BARI Mung-3,BU Mung-4,BMX-05001)mung bean genotypes under wellwatered(WW)and water deficit(WD)conditions.The WW treatment maintained sufficient soil moisture(22%±0.5%,i.e.,30%deficit of available water)by regularly supplying water.Whereas,the WD treatment was maintained throughout the growing period,and water was applied when the wilting symptom appeared.The drought tolerant(DT)genotypes BARI Mung-8,BMX-08010-2,BMX-010015,BMX-08009-7 showed a high level of proline accumulation(2.52–5.99 mg g^(−1) FW),photosynthetic pigment(total chlorophyll 2.96–3.27 mg g^(−1) FW at flowering stage,and 1.62–2.38 mg g^(−1) FW at pod developing stage),plant water relation attributes including relative water content(RWC)(82%–84%),water retention capacity(WRC)(12–14)as well as lower water saturation deficit(WSD)(19%–23%),and water uptake capacity(WUC)(2.58–2.89)under WD condition,which provided consequently higher relative seed yield.These indicate that the tolerant genotypes gained better physiobiochemical attributes and adaptability in response to drought conditions.Furthermore,the genotype BMX-08010-2 showed superiority in terms of those physio-biochemical traits,susceptibility index(SSI)and stress tolerance index(STI)to other genotypes.Based on the physiological and biochemical responses,the BMX-08010-2 was found to be a suitable genotype for sustaining yield under drought stress,and subsequently,it could be recommended for crop improvement through hybridization programs.In addition,the identified traits can be used as markers to identify tolerant genotypes for drought-prone areas.
文摘Ladakh’s cropped and non-cropped areas suffer greatly from weedy invasion under subsistence agricultural system where weeding is not a priority but total crop + weed biomass together contribute to livestock feeding significantly. As agriculture along with livestock rearing is major activity of livelihood options, thereby contributing significantly to Ladakh economy and income generation for supporting resource poor farmers. Amongst various invasive weed species, the common reed (Phragmites australis) has become a serious ecological threat for agri-silvi-pastoral system biodiversity and accelerated its expansion in cropped lands including many wetland and salt-affected habitats of cold arid region due to its intermediate plant growth habit of C3 - C4 photosynthetic ecotypes. This character makes Phragmites a climate-resilient species, which is a major challenge for cold arid agriculture in the changing climatic patterns. Simultaneously, it is one of the best fodder substitute available under harsh climate. This paper deals with its unique characters and potential threats to the agrobiodiversity of Ladakh.
基金The work was financially supported by National Agricultural Technology Program-II Project(NATP-2)BARC Component Bangladesh Agricultural Research Council,Farmgate,Dhaka-1215+2 种基金Bangladesh Agricultural Research Institute(BARI),Joydebpur,Gazipur 1701The work was partially supported by the Taif University Researchers Supporting Project No.(TURSP-2020/39)Taif University,Taif,Saudi Arabia.
文摘Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity.Therefore,molecular characterization of germplasms is important to obtain target cultivars with desirable traits.Sweet potato[Ipomoea batatas(L.)Lam]is widely considered the world’s most important crop,with great diversity in morphological and phenotypic traits.The genetic diversity of 20 sweet potato germplasms originating from Bangladesh,CIP,Philippines,Taiwan,and Malaysia were compared,which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization.This information was effective in differentiating or clustering the sweet potato genotypes.A total of 64 alleles were generated using the 20 primers throughout the 20 germplasm samples,with locus IBS97 having the highest number of alleles(5),whereas locus IbU33 had the fewest alleles(2).The alleles varied in size from 105(IbU31)to 213 base pairs(IBS34).The Polymorphism Information Content(PIC)values for the loci IbL46 and IBS97 varied from 0.445 to 0.730.IBS97 has the highest number of effective alleles(3.704),compared to an average of 2.520.The average Shannon’s diversity index(H)was 1.003,ranging from 0.673 in IbU3 to 1.432 in IBS97.The value of gene flow(Nm)varied between 0.000 and 0.005,with an average of 0.003,whereas genetic differentiation(FST-values)ranged between 0.901 and 1.000.The sweet potato germplasm included in this study had a broad genetic base.SP1 vs.SP9 and SP12 vs.SP18 germplasm pairings had the greatest genetic distance(GD=0.965),while SP1 vs.SP2 germplasm couples had the least genetic diversity(GD=0.093).Twenty genotypes were classified into two groups in the UPGMA dendrogram,with 16 genotypes classified as group“A”and the remaining four genotypes,SP10,SP18,SP19,and SP20,classified as group“B.”According to cluster analysis,the anticipated heterozygosity(gene diversity)of Nei(1973)was 0.591 on average.In summary,SSR markers successfully evaluated the genetic relationships among the sweet potato accessions used and generated a high level of polymorphism.The results of the present study will be useful for the management of germplasm,improvement of the current breeding strategies,and the release of new cultivars as varieties.
基金the funding agency ‘Forest Research Institute, Dehradun for providing necessary funds and supplying the clonesused in the study
文摘We evaluated the growth and crown traits of 36 poplar clones at two distinct agro-climatic regions of Punjab (Ludhiana and Bathinda) in northwestern India, following randomized block design with three replications and plot size of four trees. Significant differences among clones (p<0.001) were observed for diameter at breast height (DBH), tree height, volume, crown width and number of branches under both the site conditions. Clones 'G-3', '25-N' and '41-N' at Ludhiana and 'G-3', 'RD-01' and 'S 7 C 8 ' at Bathinda were found to be superior for volume production. All growth and crown traits registered significantly higher values at Ludhiana in comparison to those at Bathinda. Clone × site interaction was also significant (p<0.001). For volume, clones 'L-62/84', '113520', '25-N' and 'S 4 C 2 ' witnessed huge fluctuations in ranking between sites. The correlations between growth traits were positive and highly significant (p<0.001) at both sites. The clonal mean heritability was moderate for DBH and volume both at Ludhiana (0.61 0.66) and Bathinda (0.61 0.62). Across sites, the genetic advance was the highest for volume (49.76%) and the lowest (6.50%) in case of height.
文摘An attempt was made to observe the effect of pre-harvest bagging with spun-bound fabric bags on color and quality of Delicious apple. Bagging was done about a month before harvesting and removed 3-day before harvesting. Bagged and non-bagged fruits were stored at 2℃ ± 1℃ and 90%-95% RH. Observations were recorded on color and fruit quality attributes such as total phenolics, AOX activity, fruit Ca contents, LOX activity, SSC and ascorbic acid contents at harvest and during storage. Our studies have revealed that bagged fruits have better color development (Hunter “a” = 52) than non-bagged fruits at harvest (Hunter “a” = 38), which declined slightly during storage. Similarly, at harvest, bagged fruits contained high amounts of Ca (5.38 mg/100g) and total phenolics (9.3 mg GAE/100gpulp) exhibited higher AOX activity (12.6 μmoles Trolox g-1), and had better SSC and ascorbic acid contents than non-bagged fruits, and there was a decline in all recorded parameters during storage. Bagged fruits exhibited lower LOX activity (1.38 μmoles min-1 g-1 FW) at harvest than non-bagged fruits (2.14 μmoles min-1 g-1 FW), indicating that non-bagged fruits were more senescent than bagged fruits. Further, LOX activity increased during storage both in bagged and non-bagged apples but increase in LOX activity was slower in bagged apples than in non-bagged apples.
文摘Objective:To evaluate the insecticidal,antimicrobial and antioxidant activities of bulb extracts of Allium sativum(A.sativum).Methods:Dried bulbs of A.sativum were extracted with different solvents and evaluated for insecticidal,antimicrobial and antioxidant activities.Results: Aqueous and methanol extracts showed highest insecticidal activity(mortality rate of 81%and 64%respectively) against the larvae of Spodoptera litura(S.litura) at a concentration of 1000 ppm.With regard to antimicrobial activity,aqueous extract exhibited antibacterial activity against gram positive(Bacillus subtilis,Staphylococcus aureu,) and gram negative(Escherichia coli and Klebsiella pneumonia) strains and antifungal activity against Candida albicans.While methanol extract showed antimicrobial activity against all the tested micro organisms except two(Staphylococcus aureus and Candida albicans),the extracts of hexane,chloroform and ethyl acetate did not show any anti microbial activity.Minimum inhibitory concentration of aqueous and methanol extracts against tested bacterial and fungal strains was 100-150μg/mL. Antioxidant activity of the bulb extracts was evaluated in terms of inhibition of free radicals by 2.2’-diphenly-l-picrylhydrazyl.Aqueous and methanol extracts exhibited strong antioxidant activity(80%-90%of the standard).Conclusions:Antioxidant and antimicrobial activity of A.sativum against the tested organisms therefore,provides scientific basis for its utilization in traditional and folk medicine.Also,our results demonstrated the insecticidal efficacy of A. sativum against S.litura,a polyphagous insect.
文摘Objective:To formulate a simple rapid procedure for bioreduction of silver nanoparticles using aqueous leaves extract of Moringa oleifera(M.oleifera).Methods:10 mL of leaf extract was mixed to 90 mL of 1 mM aqueous of AgNO_3 and was heated at 60-80 ℃ for 20 min.A change from brown to reddish color was observed.Characterization using UV-Vis spectrophotometry, Transmission Electron Microscopy(TEM) was performed.Results:TEM showed the formation of silver nanoparticles with an average size of 57 nm.Conclusions:M.oleifera demonstrates strong potential for synthesis of silver nanoparticles by rapid reduction of silver ions(Ag^+ to Ag^0). Biological methods are good competents for the chemical procedures,which are eco-friendly and convenient.
文摘The presence of Al in the rhizosphere of rice in acid soil restricts root growth and significantly reduces crop productivity.In this study,the effects of Al(30,60 and 90μg/mL)on seedling root growth,number of primary roots per seedling,seedling shoot length,number of leaves per seedling,seedling fresh weight,and seedling dry weight were studied.Rice genotypes were classified into three different classes,namely,tolerant,moderately tolerant,and susceptible,based on root tolerance index.The method of hydroponic culture was modified,and elaborated in the text.Toxic levels of Al in nutrient solution significantly decreased seedling root growth,number of primary roots,seedling shoot length,number of leaves per seedling,seedling fresh weight,and seedling dry weight.Few genotypes showed longer root length at 30μg/mL Al in nutrient solutions compared with the control.High levels of Al in nutrient solutions were highly toxic for rice seedlings.Based on root tolerance index,Radhunipagal,Gobindobhog,Badshabhog,Kalobhog,UBKVR-11,UBKVR-16,UBKVR-18,Khasha and IVT4007-B were classified as tolerant genotypes,and these genotypes may be used as donors for breeding of Altoxicity tolerance.
文摘A long-term field study was initiated during 1995 at Central Soil Salinity Research Institute, Regional Research Station, Lucknow(26°47′58′′ N and 80°46′24′′ E) to analyze the effect of agroforestry systems on amelioration of alkali soils. Three agroforestry systems(pastoral, silvipastoral and silvicultural) were compared with the control where no agroforestry system was introduced. Tree-based silvicultural and silvipastoral systems were characterized by tree species Prosopis juliflora and Acacia nilotica along with grass species Leptochloa fusca, Panicum maximum, Trifolium alexandrium and Chloris gayana. Growth of ten-year-old Prosopis juliflora and Acacia nilotica planted in combination with grasses was significantly higher over the silviculture system with the same species. Tree biomass yields of P. juliflora(77.20 t?ha-1) and A. nilotica(63.20 t?ha-1) planted under silvipastoral system were significantly higher than the sole plantation of(64.50 t?ha-1 and 52.75 t?ha-1). Fodder yield under the pastoral system was significantly higher than the silvipastoral system during initial years but it was at par with that of silvipastoral systems after eight years of plantation. The microbial biomass carbon in the soils of silvipastoral systems was significantly higher than in soils under sole plantation of trees and control systems. The Prosopis-based silvipastoral system proved more effective in reducing soil pH, displacing Na+ from the exchange complex, increasing organic carbon and available N, P and K. Improvement in soil physical properties such as bulk density, porosity, soil moisture and infiltration rate was higher in the Prosopis-based silvipastoral system than in the silviculture system or control. On the basis of biomass production and improvement in soil health due to tree + grass systems, silvipastoral agroforestry system could be adopted for sustainable reclamation of highly alkali soils.
基金Department of Biotechnology, Government of India for the research grant (Grant No.BT/PR-9309/AGR/02/408/2007)
文摘Joha rice of Assam is a class of rice with aroma, good cooking qualities and excellent palatability, which are grown in different places of Assam with their adaptability to local situations. A total of 143 simple sequence repeat markers were used in this study to reveal sufficient genetic variations among the 40 Joha and 14 non-Joha rice genotypes. Polymorphism information content of these markers were from 0.17 to 0.88, where the average of 3.7 alleles were observed. The model-based population assignment and dendrogram analysis identified three distinct groups in the 54 rice accessions, which established a clear identity of Joha accessions from the Basmati accessions, indicating high diversity and strong population structure in Joha rice. Furthermore, a total of 29 significant marker-trait associations(P < 0.05) for 10 characters were detected. The QTLs related with yield and grain quality can be used effectively in crop improvement programs and for further fine mapping and validation of specific genes to develop gene-based perfect markers in rice breeding and for mining of better alleles of these genes in Joha rice collections.
文摘Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important food legume of the semi-arid tropics (SAT) sustaining livelihood of millions of people. Stagnant and unstable yield per hectare all over the world is the characteristic feature of this crop. This is primarily ascribed to its susceptibility/sensitivity to a number of biotic and abiotic factors. Among biotic factors, insects such as pod borer (Helicoverpa armigera), pod fly (Melanoagromyza obtusa) and spotted borer (Maruca vitrata) substantially damage the crop and result in significant economic losses. Management of these insects by genetic means has always been considered environment friendly approach. However, genetic improvement has always been impeded by limited genetic variability in the primary gene pool of pigeonpea. Wild species present in the secondary and tertiary gene pools have been reported to carry resistance for such insects. However, transfer of resistance through conventional backcrossing has not been much successful. It calls for gene introgression through marker assisted backcrossing (MABC) or advanced backcross breeding (AB breeding). In this review, we have attempted to assess the progress made through conventional and molecular breeding and suggested the ways to move further towards genetic enhancement for insects resistance in
文摘Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking quality.However,the yield of Varalu is substantially low since it is being affected by reproductive drought stress along with the blast disease.The genetic improvement of Varalu was done by introgressing a major yield QTL,qDTY_(12.1),along with two major blast resistance genes i.e.Pi54 and Pi1 through marker-assisted backcross breeding.Both traits were transferred till BC_(2) generation and intercrossing was followed to pyramid the two traits.Stringent foreground selection was carried out using linked markers as well as peak markers(RM28099,RM28130,RM511 and RM28163)for the targeted QTL(qDTY_(12.1)),RM206 for Pi54 and RM224 for Pi1.Extensive background selection was done using genome-wide SSR markers.Six best lines(MSM-36,MSM-49,MSM-53,MSM-57,MSM-60 and MSM-63)having qDTY_(12.1) and two blast resistance genes in homozygous condition with recurrent parent genome of 95.0%-96.5% having minimal linkage drag of about 0.1 to 0.7 Mb were identified.These lines showed yield advantage under drought stress as well as irrigated conditions.MSM-36 showed better performance in the national coordinated trials conducted across India,which indicated that improved lines of Varalu expected to replace Varalu and may have an important role in sustaining rice production.The present study demonstrated the successful marker-assisted pyramiding strategy for introgression of genes/QTLs conferring biotic stress resistance and yield under abiotic stress in rice.
文摘The common practice of establishing rice in the rice-wheat system in India is manual transplanting of seedlings in the puddled soil. Besides being costly, cumbersome, and time consuming, puddling results in degradation of soil and the formation of a hard pan, which impedes root growth of subsequent upland crops. In addition, decreased availability and increasing cost of labor have increased the cost of rice cultivation through conventional methods. Because of these concerns, there is a need for mechanized transplanting of rice which is less labor-intensive and can ensure optimum plant population under nonpuddled and/or no-till conditions. A large number of on-farm trials were conducted at farmers’ fields in Haryana, India, from 2006 to 2010 to evaluate the performance of the mechanical transplanted rice (MTR) under nonpuddled and no-till situations as compared to conventional puddled transplant rice (CPTR). Compared with CPTR, nonpuddled MTR produced 3%-11% higher grain yield in different years. Rice cultivars, viz. HKR47, HKR127, PR113, PR114, PB1, PB1121, CSR30, and Arize6129, performed consistently better under nonpuddled MTR as compared to CPTR. Performance of different cultivars (PR113, PR114, HKR47, and Pusa 44) was also better under no-till MTR as compared to CPTR. The “basmati” cultivar CSR30 performed equally in no-till MTR and CPTR systems. The results of our study suggest that rice can be easily grown under nonpuddled and no-till conditions with yield advantages over the CPTR system. Even in the case of similar yield between CPTR and MTR systems, the MTR system will help in reducing labor requirement and ultimately, will increase overall profits to farmers.
文摘The objectives of the present investigations are to see the effects of biomass power plant effluent on the carbohydrates, aminoacids, nitrite and nitrite enzyme activitiesand proline of Arachis hypogaea L. var TCGS 320 under controlled pot culture methods. Plants were cultivated with 25, 50, 75 and 100% of the effluent and a control without the effluent. The treatment of the crop with 25% of the effluent has shown stimulatory effect on all the biochemical parameters studied. Carbohydrates, starch, aminoacids, protein, nitrate and nitrite reductase enzymatic activities have increased in 10, 15, 20 DAS (days after sowing). In 25 and 30 DAS all biochemical parameters have decreased, due to environmental factors (rainfall, temperature, humidity, etc.). The present investigation clearly indicated that the biomass power plant effluent has stimulatory effect on all the biochemical contents at lower concentration, and at higher concentration they have deleterious effects.
基金This research was funded by the Bangladesh Sugar crop Research Institute(BSRI),Ishwardi,Pabna,Bangladesh and the Taif University Researchers Supporting Project No.TURSP-2020/142,Taif University,Taif,Saudi Arabia.
文摘Sugarcane is one of the major important sugar yielding crops in Bangladesh.As an exhaustive crop,sugarcane removes a huge amount of plant nutrients from the soil.However,the combined use of organic and inorganic fertilizers can be a good approach to deal with nutrient depletion and promote sustainable crop production as well as improve soil health.Therefore,an attempt was made to identify the most fruitful and profitable integrated nutrient management on the aspects of growth,yield and quality of sugarcane in two consecutive growing seasons.Seven treatments:T_(1)=Control,T_(2)=165:55:120:30:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1),T_(3)=Poultry Litter(PL)at 5 t ha^(−1)+95:51:87:9:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1),T_(4)=Cow Dung(CD)at 15 t ha^(−1)+36:52:60:17:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1),T_(5)=Press Mud(PM)at 15 t ha^(−1)+10:50:43:0:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1),T_(6)=Mustard Oil Cake(MOC)at 0.5 t ha^(−1)+140:54:115:25:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1)and T_(7)=GM(Green Manure)at 5 t ha^(−1)+140:53:100:28:10:2.5:4 kg N:P:K:S:Mg:Zn:B ha^(−1)were used in this experiment.Two years data showed that treatment T_(3)produced the maximum amount of tillers,total dry matter yield,millable sugarcane,cane yield and sugar yield,followed by the T_(4)treatment.The highest stalk heights were recorded in the T_(3)treatment,which was statistically similar to all other treatments except T_(1)and T_(2).The juice quality parameters viz.,brix and pol in cane were found significant in treatment T_(3)while the highest purity was obtained in the T_(7)treatment.All the data of Jaggery(goor)quality parameters,the highest sucrose content,color transmittance,Jaggery(goor)recovery and the lowest ash content of Jaggery(goor)were observed in the T_(3)treatment,which was statistically similar to the T_(4)treatment in both seasons.The highest cost of production was obtained from the T_(6)treatment while the highest gross return,net return and BCR were recorded in the T_(3)treatment.No significant changes were found in one cycle of sugarcane in initial and post-harvest soil characteristics viz.,pH,organic carbon,total N,and available P,K and S contents due to integrated use of different fertilizer packages.From the experimental findings,it was concluded that treatment T_(3)followed by T_(4)treatment would be the better productive and profitable integrated nutrient management technology for ensuring higher yields and quality of sugarcane without soil fertility degradation in the High Ganges River Floodplain soils.
文摘Swertia chirayita is a highly endangered and very well known medicinal plant enlisted in the IUCN list of endangered plants in the world. The plant is currently having a very low population in eastern Himalayan region due to its very low germinability in its own natural habitat. The plant population was found to have a range of diversity in morphological observations and some of the morphovariants were found to be very low among the variant populations which created a concern due to its diminishing range of genetic base in this region. A basic group of 5 morphovariants and 24 sub variants were identified and separately maintained. It is a pluriannual plant and reproductive or flowering stage was observed after completion of two years of vegetative stage from the time of seed germination. A range of morphovariant diversity was observed in the populations of Neora Valley and Lava region of eastern Himalaya in our investigation. Diversity was critically observed in some of the parameters of different morphovariants in the population. Conservation practices were generally done by not making groups or sub groups of this endangered plant while we proposed the way of conservation by making groups for proper conservation of this vulnerable plant.
基金supported jointly by Cariplo(Italia)and Agropolis(France)Foundations(Grant No.1201-006).
文摘Genome-wide association study(GWAS)was performed for 16 agronomic traits including nitrogen use efficiency(NUE)and yield-related components using a panel of 190 mainly japonica rice varieties and a set of 38390 single nucleotide polymorphism(SNP)markers.This panel was evaluated under rainfed upland conditions in Madagascar in two consecutive cropping seasons with two contrasted nitrogen input levels.Using another set of five grain traits,we identified previously known genes(GW5,GS3,Awn1 and Glabrous1),thus validating the pertinence and accuracy of our datasets for GWAS.A total of 369 significant associations were detected between SNPs and agronomic traits,gathered into 46 distinct haplotype groups and 28 isolated markers.Few association signals were identified for the complex quantitative trait NUE,however,larger number of quantitative trait loci(QTLs)were detected for its component traits,with 10 and 2 association signals for nitrogen utilization efficiency and nitrogen uptake efficiency,respectively.Several detected association signals co-localized with genes involved in nitrogen transport or nitrogen remobilization within 100 kb.The present study thus confirmed the potential of GWAS to identify candidate genes and new loci associated with agronomic traits.However,because of the quantitative and complex nature of NUE-related traits,GWAS might have not captured a large number of QTLs with limited effects.
