Sugarcane leaves-derived polyphenols(SLP)have been demonstrated to have diverse health-promoting benefits,but the mechanism of action has not been fully elucidated.This study aimed to investigate the anti-metabolic di...Sugarcane leaves-derived polyphenols(SLP)have been demonstrated to have diverse health-promoting benefits,but the mechanism of action has not been fully elucidated.This study aimed to investigate the anti-metabolic disease effects of SLP and the underlying mechanisms in mice.In the current study,we prepared the SLP mainly consisting of three flavonoid glycosides,three phenol derivatives,and two lignans including one new compound,and further demonstrated that SLP reduced body weight gain and fat accumulation,improved glucose and lipid metabolism disorders,ameliorated hepatic steatosis,and regulated short-chain fatty acids(SCFAs)production and secondary bile acids metabolism in ob/ob mice.Notably,SLP largely altered the gut microbiota composition,especially enriching the commensal bacteria Akkermansia muciniphila and Bacteroides acidifaciens.Oral gavage with the above two strains ameliorated metabolic syndrome(MetS),regulated secondary bile acid metabolism,and increased the production of SCFAs in high-fat diet(HFD)-induced obese mice.These results demonstrated that SLP could be used as a prebiotic to attenuate MetS via regulating gut microbiota composition and further activating the secondary bile acids-mediated gut-adipose axis.展开更多
A polyphenolic alkaloid-enriched extract(PAE)was prepared from the fruiting bodies of a wild edible mushroom Sarcodon leucopus.Oral administration of PAE reduced hyperglycemia,hyperlipidemia,hepatic steatosis,and LPS-...A polyphenolic alkaloid-enriched extract(PAE)was prepared from the fruiting bodies of a wild edible mushroom Sarcodon leucopus.Oral administration of PAE reduced hyperglycemia,hyperlipidemia,hepatic steatosis,and LPS-related infl ammation in high fat diet-induced obese(DIO)mice.Furthermore,we show that PAE produces taxonomic and predicted functional changes in the gut microbiome of DIO mice.A signifi cant decrease in the family of Ruminococcaceae,especially the secondary bile acid-producing bacteria of Intestinimonas and Anaerotruncus,is detected in the gut microbiome of PAE-treate mice.Accordingly,reductions of deoxycholic acid and lithocholic acid are found in the feces of PAE-treated DIO mice,which benefi ts for the intestinal integrity and the reduction of infl ammation.A gut microbiota related mechanism for the anti-metabolic syndrome effects of the PAE is proposed.We suppose the polyphenolic alkaloid extract from S.leucopus be a new and benefi cial prebiotic regulating glucose and lipid metabolisms.展开更多
Two new meroterpenoid compounds(1 and 2) together with five known meroterpenoid derivatives(3–7) were isolated from solid culture of mushroom Panus lecomtei. The structures of new compounds were confirmed by the anal...Two new meroterpenoid compounds(1 and 2) together with five known meroterpenoid derivatives(3–7) were isolated from solid culture of mushroom Panus lecomtei. The structures of new compounds were confirmed by the analysis of NMR and HRESI-MS spectroscopic data. The biosynthetic pathway of 1–7 was postulated. All isolated compounds were evaluated for antibacterial activities against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Bacillus Calmette–Guérin.Compound 3 exhibited weak antibacterial activity against Bacillus Calmette–Guérin with the inhibition rate of 83.6% at 100 μmol·L-1.Other compounds showed no antibacterial activities against all tested pathogens at 100 μmol·L-1.展开更多
基金supported by the National key research and development program of China(2019YFA0905600)the Science and Technology Service Network Program of the Chinese Academy of Sciences(KFJ-STS-QYZD-201-5-3)the Strategic Priority Research Program(Class B)of Chinese Academy of Sciences(XDB 38020300)。
文摘Sugarcane leaves-derived polyphenols(SLP)have been demonstrated to have diverse health-promoting benefits,but the mechanism of action has not been fully elucidated.This study aimed to investigate the anti-metabolic disease effects of SLP and the underlying mechanisms in mice.In the current study,we prepared the SLP mainly consisting of three flavonoid glycosides,three phenol derivatives,and two lignans including one new compound,and further demonstrated that SLP reduced body weight gain and fat accumulation,improved glucose and lipid metabolism disorders,ameliorated hepatic steatosis,and regulated short-chain fatty acids(SCFAs)production and secondary bile acids metabolism in ob/ob mice.Notably,SLP largely altered the gut microbiota composition,especially enriching the commensal bacteria Akkermansia muciniphila and Bacteroides acidifaciens.Oral gavage with the above two strains ameliorated metabolic syndrome(MetS),regulated secondary bile acid metabolism,and increased the production of SCFAs in high-fat diet(HFD)-induced obese mice.These results demonstrated that SLP could be used as a prebiotic to attenuate MetS via regulating gut microbiota composition and further activating the secondary bile acids-mediated gut-adipose axis.
基金This study was supported by the Project of Science and Technology Department of Tibet(XZ201901-GB-19).
文摘A polyphenolic alkaloid-enriched extract(PAE)was prepared from the fruiting bodies of a wild edible mushroom Sarcodon leucopus.Oral administration of PAE reduced hyperglycemia,hyperlipidemia,hepatic steatosis,and LPS-related infl ammation in high fat diet-induced obese(DIO)mice.Furthermore,we show that PAE produces taxonomic and predicted functional changes in the gut microbiome of DIO mice.A signifi cant decrease in the family of Ruminococcaceae,especially the secondary bile acid-producing bacteria of Intestinimonas and Anaerotruncus,is detected in the gut microbiome of PAE-treate mice.Accordingly,reductions of deoxycholic acid and lithocholic acid are found in the feces of PAE-treated DIO mice,which benefi ts for the intestinal integrity and the reduction of infl ammation.A gut microbiota related mechanism for the anti-metabolic syndrome effects of the PAE is proposed.We suppose the polyphenolic alkaloid extract from S.leucopus be a new and benefi cial prebiotic regulating glucose and lipid metabolisms.
基金National Key R&D Program of China (Nos. 2018YFD0400203,2017YFE0108200 and2017YFE0108100)the National Natural Science Foundation of China (Nos. 81673334 and 31772374)。
文摘Two new meroterpenoid compounds(1 and 2) together with five known meroterpenoid derivatives(3–7) were isolated from solid culture of mushroom Panus lecomtei. The structures of new compounds were confirmed by the analysis of NMR and HRESI-MS spectroscopic data. The biosynthetic pathway of 1–7 was postulated. All isolated compounds were evaluated for antibacterial activities against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Bacillus Calmette–Guérin.Compound 3 exhibited weak antibacterial activity against Bacillus Calmette–Guérin with the inhibition rate of 83.6% at 100 μmol·L-1.Other compounds showed no antibacterial activities against all tested pathogens at 100 μmol·L-1.
