Background:In order to clarify the inmpat ofγirradiation on the chemical composition of traditional Chinese medicine,this paper carefully choosed Chuanxiong Rhizoma to carry on a demonstration study.Methods:Through a...Background:In order to clarify the inmpat ofγirradiation on the chemical composition of traditional Chinese medicine,this paper carefully choosed Chuanxiong Rhizoma to carry on a demonstration study.Methods:Through a meticulous assessment,a comprehensive comparison was made between the irradiated and unirradiated Chuanxiong Rhizoma samples.The property characteristics were investigated by colorimeter and electronic nose.The changes in chemical structures and contents was analyzed by fourier infrared spectroscopy,high performance liquid chromatography and fingerprinting.In a quest to uncover the presence of any new radiolysis products,cutting-edge techniques like ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry and gas chromatography-mass spectrometry were employed.Moreover,the difference of antioxidant activity were investigated.Results:The irradiation doses within 12 kGy had no significant effects on the content of the main chemical components,characteristics and in vitro antioxidant activity of Chuanxiong Rhizoma,while changes in some functional groups and degradation of some volatile oil components containing olefins need further study.Conclusion:This study indicates that^(60)Co-γirradiation is a stable method for sterilization of Chuanxiong Rhizoma.It’s also provide a reference for the establishment of irradiation standards for Chuanxiong Rhizoma and other aromatic medicinal plants.展开更多
Chinese minority traditional medicine is a traditional medicine formed during the long-term medical practice of various ethnic groups and is the crystallization of the wisdom of the people in all ethnic groups in Chin...Chinese minority traditional medicine is a traditional medicine formed during the long-term medical practice of various ethnic groups and is the crystallization of the wisdom of the people in all ethnic groups in China.The unique meaning carried by Chinese minority traditional medicine makes it an inseparable and important part of traditional Chinese medicine.The rich clinical experience and theoretical studies with different Chinese minority traditional medicine provide new ideas and new methods for Chinese medicine development.The pieces of literature published in 2020 on the traditional application analyzed the phytochemical composition,pharmacological effects,and mechanisms of the different ethnic groups including Tibetan,Mongolian,Zhuang,Dai,and Uyghur medicines,among others.This study found that some projects were stalled or delayed due to the coronavirus disease 2019 outbreak in 2020 compared with 2019,resulting in a decrease in the number of articles in certain fields.However,studied on Chinese minority traditional medicine have shown an overall upward trend this year with the Tibetan,Mongolian,and Dai medicines as the top three.In addition,the Chinese minority traditional medicine application under the coronavirus disease 2019 epidemic situation background was also summarized.Consequently,this study found that among the Chinese minority traditional medicine-related publications in 2020,publications using Chinese minority traditional medicine to treat coronavirus disease 2019 account for a certain percentage.Thus,this article systematically reviews and summarizes the related research of Chinese minority traditional medicine published in 2020 and provides references for future Chinese minority traditional medicine development.展开更多
[Objectives]To establish the quality standard of Tibetan medicine Corydalis dasyptera Maxim.[Methods]According to the research method of drug quality standard in the appendix of 2020 edition of Chinese Pharmacopoeia,8...[Objectives]To establish the quality standard of Tibetan medicine Corydalis dasyptera Maxim.[Methods]According to the research method of drug quality standard in the appendix of 2020 edition of Chinese Pharmacopoeia,8 batches of C.dasyptera Maxim.from different habitats were studied by character identification,microscopic identification and TLC identification.The content of water,total ash,acid-insoluble ash and alcohol-soluble extract was determined,and the content of protopine in medicinal materials was determined by high performance liquid chromatography(HPLC).[Results]The properties and microscopic characteristics of C.dasyptera Maxim.were determined.The TLC characteristic spots of the medicinal materials were clear,the degree of separation was good,and the specificity was strong.Both the test sample and the control sample showed the same yellow-green spots in the corresponding position.It was tentatively determined that the water content of C.dasyptera Maxim.should not exceed 14.0%,the total ash content should not exceed 14.0%,the acid-insoluble ash content should not exceed 3.0%,and the alcohol-soluble extract content should not be less than 18.0%.There was a good linear relationship between the concentration of protopine and the peak area in the range of 16.64-166.40μg·10-3(r=0.9996).The average recovery rate was 98.47%and the RSD was 1.21%(n=6).The content of protopine in 8 batches of C.dasyptera Maxim.was 0.023%-0.093%.[Conclusions]The established quality research method is simple,stable and reliable,and can be used for the quality control of C.dasyptera Maxim.展开更多
A scale-up fermentation of the fungus Boreostereum vibrans facilitated the isolation of six new vibralactone biogenesis-associated analogues,namely vibralactamide A(1),vibralactone T(2),13-O-lactyl vibralactone(3),10-...A scale-up fermentation of the fungus Boreostereum vibrans facilitated the isolation of six new vibralactone biogenesis-associated analogues,namely vibralactamide A(1),vibralactone T(2),13-O-lactyl vibralactone(3),10-O-acetyl vibralactone G(4),(11R,12R)-and(11S,12R)-vibradiol(5,6).Their structures were established via extensive spectroscopic analyses,specific optical rotation comparison,and Snatzke’s method.The biosynthetic pathway for vibralactamide A was postulated.The absolute configuration of vibralactone B was revised by single crystal X-ray diffraction analysis.This work puts the divergent vibralactone biosynthesis pathway one step further and expands the structural diversity of vibralactone-associated compounds.展开更多
Background:Chinese minority traditional medicine(CMTM)is an important component of Chinese traditional culture and medicine that has contributed to the health and prosperity of Chinese people of all ethnic groups.Alth...Background:Chinese minority traditional medicine(CMTM)is an important component of Chinese traditional culture and medicine that has contributed to the health and prosperity of Chinese people of all ethnic groups.Although this type of medicine has attracted interest,its developmental trends and research status remain unclear.Therefore,we used literature metrology to evaluate the publications on CMTM during 2021 and explore hotspots and frontier areas.Methods:Reports associated with CMTM published between January 1,2021,and December 31,2021,were downloaded from the Web of Science database.The authors,institutions,countries,journals,keywords,and other standard bibliometric indicators were analyzed and visualized using CiteSpace,and research hotspots and trends were identified.Results:We retrieved 152 CMTM-related publications,among which Tibetan medicine attracted the most interest.Journals covering alternative and complementary medicine topics were the most prevalent sources of reports about CMTM,particularly the Journal of Ethnopharmacology.Most research in this field was conducted in China and frequently published by traditional Chinese medicine colleges and universities.One author,Yi Zhang,has extensively investigated Tibetan and Korean medicine.The keyword co-occurrence network revealed that investigations into ethnic groups mainly focused on the pharmacological activities and phytochemical components of ethnic medicines.The keywords of gut microbiota and autophagy in some publications reflect the direction of future CMTM investigations.Conclusion:Our findings showed the current status and trends in CMTM investigations.This information will help identify new research directions and hotspots in this field.展开更多
The regenerative capacity of the central nervous system is very limited and few effective treatments are currently available for spinal cord injury.It is therefore a priority to develop new drugs that can promote stru...The regenerative capacity of the central nervous system is very limited and few effective treatments are currently available for spinal cord injury.It is therefore a priority to develop new drugs that can promote structural and functional recovery after spinal cord injury.Previous studies have shown that peptides can promote substantial repair and regeneration of injured tissue.While amphibians have a pronounced ability to regenerate the spinal cord,few studies have investigated the effect of amphibian spinal cord-derived peptides on spinal cord injury.Here we report for the first time the successful identification and isolation of a new polypeptide,VD11(amino acid sequence:VDELWPPWLPC),from the spinal cord of an endemic Chinese amphibian(Odorrana schmackeri).In vitro experiments showed that VD11 promoted the secretion of nerve growth factor and brain-derived neurotrophic factor in BV2 cells stimulated with lipopolysaccharide,as well as the proliferation and synaptic elongation of PC12 cells subjected to hypoxia.In vivo experiments showed that intravertebral injection of VD11 markedly promoted recovery of motor function in rats with spinal cord injury,alleviated pathological damage,and promoted axonal regeneration.Furthermore,RNA sequencing and western blotting showed that VD11 may affect spinal cord injury through activation of the AMPK and AKT signaling pathways.In summary,we discovered a novel amphibian-derived peptide that promotes structural and functional recovery after spinal cord injury.展开更多
Objective:Rheumatoid arthritis(RA)is a systemic autoimmune disease characterized by chronic erosive arthritis.Due to the lack of effective biomarkers for diagnosis and treatment,RA patients have many complications in ...Objective:Rheumatoid arthritis(RA)is a systemic autoimmune disease characterized by chronic erosive arthritis.Due to the lack of effective biomarkers for diagnosis and treatment,RA patients have many complications in the later stage,seriously affecting their quality of life.Thus,this study was conducted to investigate new therapeutic targets and to discover diagnostic biomarkers in RA.Methods:In this study,the expression profiles of GSE55235 and GSE55457 were downloaded from the Gene Expression Omnibus database to obtain DEGs between RA and healthy samples.Genetic Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on the common genes existing in the RA-related modules.Additionally,we used the STRING database to construct the protein‒protein interaction network.Furthermore,we established the interaction analysis of Hub Genes and microRNA(miRNA)and verified the 10 Hub genes through the GSE77298 dataset and quantitative real-time polymerase chain reaction Results:276 and 69 DEGs were screened from the GSE55235 dataset and GSE55457 dataset,respectively.Then,we obtained 42 up-regulated genes in two chip datasets intersection.Genetic Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the 42 up-regulated genes showed that they were mainly concentrated in immune response-activating cell surface receptor signaling pathway,etc.Furthermore,the protein-protein interaction network indicated that 10 hub genes are closely related to RA,including MS4A1,CD27,LCK,CD79A,SDC1,CXCL9,CXCL10,CXCL13,IGLL5,and IGJ.In addition,we found that miR-4531 is the same target miRNAs between MS4A1 and SDC1 through messenger RNA-miRNA co-expression network.Finally,the GSE77298 gene chip and quantitative real-time polymerase chain reaction verified the expression of 10 Hub genes.The six Hub genes of CD27,SDC1,CXCL9,CXCL10,CXCL13,and IGJ are significantly increased.Conclusions:We found that SDC1 may be a novel molecular marker for the prevention and treatment of RA.The miR-4531/SDC1 regulatory axis may play a key role in this process.In conclusion,our study not only provides potential biomarkers for the diagnosis and treatment of RA,but also provides a basis and new targets for further revealing the potential mechanism of RA occurrence and development and discovering targeted drugs.展开更多
Natural products are the important source for the discovery of more potent anti-HIV agents.In this study,six daphnane diterpenoids including three unreported structures were isolated from Trigonostemon lii,which showe...Natural products are the important source for the discovery of more potent anti-HIV agents.In this study,six daphnane diterpenoids including three unreported structures were isolated from Trigonostemon lii,which showed signifcant activities against HIV-1 strains replication in the nanomolar/picomolar range.Meanwhile,these diterpenoids signifcantly inhibited the fusion of H9/HIV-1 IIIB cells with uninfected C8166 cells,with the EC50s from 1.06 to 8.73 ng/mL,and did not show any inhibition activities against HIV-1 reverse transcriptase.Moreover,all of the diterpenoids shows signifcant inhibitions against T20-resistan HIV-1 strains,PNL4-3gp41(36G)V38E,N42S and pNL4-3gp41(36G)V38A,N42T.The results revealed that the six diterpenoids could be a new type of potential lead candidate as an HIV entry inhibitor,particularly for those infected by T20-resistant variants.展开更多
Objective:The transformations that occur in diterpenoid alkaloids during the process of sand frying for Chinese herbal medicine preparation have yet to be clarified.This study investigated the structural changes that ...Objective:The transformations that occur in diterpenoid alkaloids during the process of sand frying for Chinese herbal medicine preparation have yet to be clarified.This study investigated the structural changes that take place in 3-acetylaconitine during a simulation of heat-processing and evaluated the toxicity and biological activity of the pyrolysis products.Methods:The diterpenoid alkaloid 3-acetylaconitine was heated at 180°C for 15 min to simulate the process of sand frying.The pyrolysis products were separated using column chromatography,and their structures were investigated using high-resolution electrospray ionization mass spectroscopy and nuclear magnetic resonance spectroscopy.Further,in vivo cardiotoxicity and acute toxicity of 3-acetylaconitine and its pyrolysis products were compared,and the aconitine-induced arrhythmia model was employed to evaluate the antiarrhythmic effect of the pyrolysis products.Results:Two new diterpenoid alkaloids,pyroacetylaconitine and 16-epi-pyroacetylaconitine,a pair of epimers at C-16,were isolated.After comparing the structures of these compounds,possible transformation pathways were proposed.Compared with the prototype compound,3-acetylaconitine,the cardiotoxicity and acute toxicity of the heat-transformed products were significantly decreased.In the biological activity assay,the two pyrolysis products exhibited an effective increase in ventricular premature beat latency,a reduction in the occurrence of ventricular tachycardia,as well as an increase in the rate of arrhythmia inhibition,implying strong antiarrhythmic activity.Conclusion:Compared with 3-acetylaconitine,its pyrolysis products displayed lower toxicity and good antiarrhythmic effects;thus,they have potential for being developed into antiarrhythmic medicines.展开更多
OBJECTIVE:To investigate the effect of ethanol extracts of Muxiang(Radix Aucklandiae)(RA)on gastric ulcers in rats and explore the potential mechanisms.METHODS:A model was established by ethanol(0.75 m L/kg).According...OBJECTIVE:To investigate the effect of ethanol extracts of Muxiang(Radix Aucklandiae)(RA)on gastric ulcers in rats and explore the potential mechanisms.METHODS:A model was established by ethanol(0.75 m L/kg).According to body weight,rats were pretreated with RA extracts(2.5 or 5 g/kg).The rats were administered 95%ethanol orally after 1 h.The effects of ethanol were evaluated by measuring the gastric secretion volume,p H,pepsin activity,and ulcer area.Histological analysis and immunohistochemistry were also conducted.Furthermore,the effect of the ethanol extract of RA on transiting activity of the gastrointestinal tract was observed in mice.RESULTS:Intragastric administration of RA extracts protected the gastric mucosa from ethanol-induced gastric ulcers,while reducing submucosal edema and preventing hemorrhagic damage.Moreover,the extracts increased the production of gastric mucus,upregulated Bcl-2,and downregulated Bax expression.Importantly,pretreated rats exhibited no significant change in the gastric secretion volume,gastric juice acidity,or pepsin.Furthermore,pretreatment prominently(P<0.05)enhanced propulsive movement of the gastrointestinal tract in normal mice and mice with gastrointestinal motility disorders.CONCLUSION:Ethanol extracts of RA ameliorated gastric lesions in the gastric ulcer rat model.The mechanisms of action were related to improvement of gastrointestinal dynamics,maintenance of mucus integrity,and inhibition of apoptosis by downregulating proapoptotic Bax protein and upregulating anti-apoptotic Bcl-2 protein.展开更多
Objective:To investigate the effect of seabuckthorn berries extract(SBE)on pulmonary vascular hyperpermeability in the mice model of acute lung injury(ALI)induced by lipopolysaccharide(LPS).Methods:Sixty Kunming mice ...Objective:To investigate the effect of seabuckthorn berries extract(SBE)on pulmonary vascular hyperpermeability in the mice model of acute lung injury(ALI)induced by lipopolysaccharide(LPS).Methods:Sixty Kunming mice were allocated into 6 groups by a random number table,including control,LPS,dexamethasone(Dex,1 mg/kg),and 120,240 and 480 mg/kg SBE groups,10 mice in each group.Except the control group,mice were pre-treated with Dex and SBE,respectively,for 7 days before LPS was intraperitoneally injected to induce ALl.Pulmonary vascular hyperpermeabilitywas evaluated by histopathologic observation and transvascular leakagedetermination.Tumor necrosis factor alpha(TNF-α)and interleukin-6(IL-6)levels in serum were measured using enzyme-linked immunosorbent assay.The expression of nuclear factor-kappa B(NF-k B)p65 in lung cells was determined by immunofluorescence analysis.The contents of cytoplasmic inhibitorof nuclear factor-kBkinase(IKK)and nuclearp65,as wellasdownstreamproteins of E-selectin(CD62E)and intercellular adhesion molecule-1(ICAM-1),were determined using Western blot analysis.Results:Histopathological observation confirmed SBEtreatment alleviatedmorphological lesion induced by LPS.Compared with the LPS group,480 mg/kg SBE significantly decreased the water content of lung,Evans blue accumulation in lung tissue,and protein concentration and neutrophils count in bronchoalveolar lavage fluid(P<o.01);moreover,480 mg/kg SBE significantly suppressed release of TNF-αand IL-6,and down-regulated expressions of IKK,nuclear p65,ICAM-1 and CD62E(P<0.01).Conclusion:SBE maintained alveolar-capillary barrier integrity under endotoxin challenge in mice by suppressing the key factors in the pathogenesis of ALl.展开更多
基金This work was financially supported by Nationalities Introduces Talented Research Startup Project of Southwest Minzu University(RQD2021055)Sichuan Science and Technology Program(R22ZYZF0005)Innovative Scientific Research Project for Postgraduates of Southwest Minzu University(ZD2022798).
文摘Background:In order to clarify the inmpat ofγirradiation on the chemical composition of traditional Chinese medicine,this paper carefully choosed Chuanxiong Rhizoma to carry on a demonstration study.Methods:Through a meticulous assessment,a comprehensive comparison was made between the irradiated and unirradiated Chuanxiong Rhizoma samples.The property characteristics were investigated by colorimeter and electronic nose.The changes in chemical structures and contents was analyzed by fourier infrared spectroscopy,high performance liquid chromatography and fingerprinting.In a quest to uncover the presence of any new radiolysis products,cutting-edge techniques like ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry and gas chromatography-mass spectrometry were employed.Moreover,the difference of antioxidant activity were investigated.Results:The irradiation doses within 12 kGy had no significant effects on the content of the main chemical components,characteristics and in vitro antioxidant activity of Chuanxiong Rhizoma,while changes in some functional groups and degradation of some volatile oil components containing olefins need further study.Conclusion:This study indicates that^(60)Co-γirradiation is a stable method for sterilization of Chuanxiong Rhizoma.It’s also provide a reference for the establishment of irradiation standards for Chuanxiong Rhizoma and other aromatic medicinal plants.
基金supported by the National Key R&D Program of China(No.2017YFC1703900)the introduction and cultivation plan of young innovative talents in Colleges and Universities of Shandong Province in 2019(No.2019-81)+2 种基金the Social Science Popularization and Application Project of Shandong Province(No.2020-SKZZ-43)the Program of Scientific Research Project of Qingdao Binhai University(No.2020KZ01,2019KY13)Qingdao Science and Technology Demonstration and Guidance Project(No.20-3-4-52-nsh).
文摘Chinese minority traditional medicine is a traditional medicine formed during the long-term medical practice of various ethnic groups and is the crystallization of the wisdom of the people in all ethnic groups in China.The unique meaning carried by Chinese minority traditional medicine makes it an inseparable and important part of traditional Chinese medicine.The rich clinical experience and theoretical studies with different Chinese minority traditional medicine provide new ideas and new methods for Chinese medicine development.The pieces of literature published in 2020 on the traditional application analyzed the phytochemical composition,pharmacological effects,and mechanisms of the different ethnic groups including Tibetan,Mongolian,Zhuang,Dai,and Uyghur medicines,among others.This study found that some projects were stalled or delayed due to the coronavirus disease 2019 outbreak in 2020 compared with 2019,resulting in a decrease in the number of articles in certain fields.However,studied on Chinese minority traditional medicine have shown an overall upward trend this year with the Tibetan,Mongolian,and Dai medicines as the top three.In addition,the Chinese minority traditional medicine application under the coronavirus disease 2019 epidemic situation background was also summarized.Consequently,this study found that among the Chinese minority traditional medicine-related publications in 2020,publications using Chinese minority traditional medicine to treat coronavirus disease 2019 account for a certain percentage.Thus,this article systematically reviews and summarizes the related research of Chinese minority traditional medicine published in 2020 and provides references for future Chinese minority traditional medicine development.
基金Chunhui Project—Identification of the Source of Tibetan Medicine C.dasyptera Maxim.
文摘[Objectives]To establish the quality standard of Tibetan medicine Corydalis dasyptera Maxim.[Methods]According to the research method of drug quality standard in the appendix of 2020 edition of Chinese Pharmacopoeia,8 batches of C.dasyptera Maxim.from different habitats were studied by character identification,microscopic identification and TLC identification.The content of water,total ash,acid-insoluble ash and alcohol-soluble extract was determined,and the content of protopine in medicinal materials was determined by high performance liquid chromatography(HPLC).[Results]The properties and microscopic characteristics of C.dasyptera Maxim.were determined.The TLC characteristic spots of the medicinal materials were clear,the degree of separation was good,and the specificity was strong.Both the test sample and the control sample showed the same yellow-green spots in the corresponding position.It was tentatively determined that the water content of C.dasyptera Maxim.should not exceed 14.0%,the total ash content should not exceed 14.0%,the acid-insoluble ash content should not exceed 3.0%,and the alcohol-soluble extract content should not be less than 18.0%.There was a good linear relationship between the concentration of protopine and the peak area in the range of 16.64-166.40μg·10-3(r=0.9996).The average recovery rate was 98.47%and the RSD was 1.21%(n=6).The content of protopine in 8 batches of C.dasyptera Maxim.was 0.023%-0.093%.[Conclusions]The established quality research method is simple,stable and reliable,and can be used for the quality control of C.dasyptera Maxim.
基金supported by National Natural Science Foundation of China(No.81561148013).
文摘A scale-up fermentation of the fungus Boreostereum vibrans facilitated the isolation of six new vibralactone biogenesis-associated analogues,namely vibralactamide A(1),vibralactone T(2),13-O-lactyl vibralactone(3),10-O-acetyl vibralactone G(4),(11R,12R)-and(11S,12R)-vibradiol(5,6).Their structures were established via extensive spectroscopic analyses,specific optical rotation comparison,and Snatzke’s method.The biosynthetic pathway for vibralactamide A was postulated.The absolute configuration of vibralactone B was revised by single crystal X-ray diffraction analysis.This work puts the divergent vibralactone biosynthesis pathway one step further and expands the structural diversity of vibralactone-associated compounds.
基金supported by the National Natural Science Foundation of China(No.82104534)the National Key R&D Program of China(No.2017YFC1703900)+2 种基金the Social Science Popularization and Application Project of Shandong Province(No.2020-SKZZ-43)the Regional Innovation and Cooperation Project of the Science&Technology Department of Sichuan Province(No.2020YFQ0032)the Key R&D and Transformation Program of the Science&Technology Department of Qinghai Province(No.2020-SF-C33).
文摘Background:Chinese minority traditional medicine(CMTM)is an important component of Chinese traditional culture and medicine that has contributed to the health and prosperity of Chinese people of all ethnic groups.Although this type of medicine has attracted interest,its developmental trends and research status remain unclear.Therefore,we used literature metrology to evaluate the publications on CMTM during 2021 and explore hotspots and frontier areas.Methods:Reports associated with CMTM published between January 1,2021,and December 31,2021,were downloaded from the Web of Science database.The authors,institutions,countries,journals,keywords,and other standard bibliometric indicators were analyzed and visualized using CiteSpace,and research hotspots and trends were identified.Results:We retrieved 152 CMTM-related publications,among which Tibetan medicine attracted the most interest.Journals covering alternative and complementary medicine topics were the most prevalent sources of reports about CMTM,particularly the Journal of Ethnopharmacology.Most research in this field was conducted in China and frequently published by traditional Chinese medicine colleges and universities.One author,Yi Zhang,has extensively investigated Tibetan and Korean medicine.The keyword co-occurrence network revealed that investigations into ethnic groups mainly focused on the pharmacological activities and phytochemical components of ethnic medicines.The keywords of gut microbiota and autophagy in some publications reflect the direction of future CMTM investigations.Conclusion:Our findings showed the current status and trends in CMTM investigations.This information will help identify new research directions and hotspots in this field.
基金supported by the National Natural Science Foundation of China,Nos.32060212(to YW),81760648(to XWY),81560118(to BYZ)Project of Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation,Nos.202101AY070001-006(to XWY)and 2018FE001(-161)(to JS)+2 种基金Yunnan Applied Basic Research Project Foundation,No.2019FB128(to YW)Project of Yunnan Province Clinical Research Centerfor Chronic Kidney Disease,No.202102AA10060(to BYZ)a grant from Scientific Research Foundation of Department of Education of Yunnan Province,No.2021J0205(to SSL)。
文摘The regenerative capacity of the central nervous system is very limited and few effective treatments are currently available for spinal cord injury.It is therefore a priority to develop new drugs that can promote structural and functional recovery after spinal cord injury.Previous studies have shown that peptides can promote substantial repair and regeneration of injured tissue.While amphibians have a pronounced ability to regenerate the spinal cord,few studies have investigated the effect of amphibian spinal cord-derived peptides on spinal cord injury.Here we report for the first time the successful identification and isolation of a new polypeptide,VD11(amino acid sequence:VDELWPPWLPC),from the spinal cord of an endemic Chinese amphibian(Odorrana schmackeri).In vitro experiments showed that VD11 promoted the secretion of nerve growth factor and brain-derived neurotrophic factor in BV2 cells stimulated with lipopolysaccharide,as well as the proliferation and synaptic elongation of PC12 cells subjected to hypoxia.In vivo experiments showed that intravertebral injection of VD11 markedly promoted recovery of motor function in rats with spinal cord injury,alleviated pathological damage,and promoted axonal regeneration.Furthermore,RNA sequencing and western blotting showed that VD11 may affect spinal cord injury through activation of the AMPK and AKT signaling pathways.In summary,we discovered a novel amphibian-derived peptide that promotes structural and functional recovery after spinal cord injury.
基金This work was supported by grants from the National Natural Science Foundation of China(82130113)the National Key R&D Program of China(2017YFC1703904)+2 种基金the Regional Innovation and Cooperation Project of the Science&Technology Department of Sichuan Province(2020YFQ0032)the Key R&D and Transformation Program of the Science&Technology Department of Qinghai Province(2020-SF-C33)the“Xinglin Scholars”Research Promotion Program of Chengdu University of Traditional Chinese Medicine(BSH2021008).
文摘Objective:Rheumatoid arthritis(RA)is a systemic autoimmune disease characterized by chronic erosive arthritis.Due to the lack of effective biomarkers for diagnosis and treatment,RA patients have many complications in the later stage,seriously affecting their quality of life.Thus,this study was conducted to investigate new therapeutic targets and to discover diagnostic biomarkers in RA.Methods:In this study,the expression profiles of GSE55235 and GSE55457 were downloaded from the Gene Expression Omnibus database to obtain DEGs between RA and healthy samples.Genetic Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on the common genes existing in the RA-related modules.Additionally,we used the STRING database to construct the protein‒protein interaction network.Furthermore,we established the interaction analysis of Hub Genes and microRNA(miRNA)and verified the 10 Hub genes through the GSE77298 dataset and quantitative real-time polymerase chain reaction Results:276 and 69 DEGs were screened from the GSE55235 dataset and GSE55457 dataset,respectively.Then,we obtained 42 up-regulated genes in two chip datasets intersection.Genetic Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the 42 up-regulated genes showed that they were mainly concentrated in immune response-activating cell surface receptor signaling pathway,etc.Furthermore,the protein-protein interaction network indicated that 10 hub genes are closely related to RA,including MS4A1,CD27,LCK,CD79A,SDC1,CXCL9,CXCL10,CXCL13,IGLL5,and IGJ.In addition,we found that miR-4531 is the same target miRNAs between MS4A1 and SDC1 through messenger RNA-miRNA co-expression network.Finally,the GSE77298 gene chip and quantitative real-time polymerase chain reaction verified the expression of 10 Hub genes.The six Hub genes of CD27,SDC1,CXCL9,CXCL10,CXCL13,and IGJ are significantly increased.Conclusions:We found that SDC1 may be a novel molecular marker for the prevention and treatment of RA.The miR-4531/SDC1 regulatory axis may play a key role in this process.In conclusion,our study not only provides potential biomarkers for the diagnosis and treatment of RA,but also provides a basis and new targets for further revealing the potential mechanism of RA occurrence and development and discovering targeted drugs.
基金supported financially by grants from the National Science Foundation of China(21432010 and 81660612)Technological leading talent project of Yunnan(2015HA020)+1 种基金Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation(2017FE467(-127),Scientific Research Fund Projects from the Department of Education of Yunnan(2016ZDX042)the Hundred-Talent Program of Kunming Medical University(60117190441).
文摘Natural products are the important source for the discovery of more potent anti-HIV agents.In this study,six daphnane diterpenoids including three unreported structures were isolated from Trigonostemon lii,which showed signifcant activities against HIV-1 strains replication in the nanomolar/picomolar range.Meanwhile,these diterpenoids signifcantly inhibited the fusion of H9/HIV-1 IIIB cells with uninfected C8166 cells,with the EC50s from 1.06 to 8.73 ng/mL,and did not show any inhibition activities against HIV-1 reverse transcriptase.Moreover,all of the diterpenoids shows signifcant inhibitions against T20-resistan HIV-1 strains,PNL4-3gp41(36G)V38E,N42S and pNL4-3gp41(36G)V38A,N42T.The results revealed that the six diterpenoids could be a new type of potential lead candidate as an HIV entry inhibitor,particularly for those infected by T20-resistant variants.
基金supported by the National Natural Science Foundation of China(No.81403104)the Sichuan Science and Technology Program(No.2020YJ0131)Xinglin Scholar Research Promotion Project of Chengdu University of Traditional Chinese Medicine,China(No.QNXZ2018042,No.QJRC2022046).
文摘Objective:The transformations that occur in diterpenoid alkaloids during the process of sand frying for Chinese herbal medicine preparation have yet to be clarified.This study investigated the structural changes that take place in 3-acetylaconitine during a simulation of heat-processing and evaluated the toxicity and biological activity of the pyrolysis products.Methods:The diterpenoid alkaloid 3-acetylaconitine was heated at 180°C for 15 min to simulate the process of sand frying.The pyrolysis products were separated using column chromatography,and their structures were investigated using high-resolution electrospray ionization mass spectroscopy and nuclear magnetic resonance spectroscopy.Further,in vivo cardiotoxicity and acute toxicity of 3-acetylaconitine and its pyrolysis products were compared,and the aconitine-induced arrhythmia model was employed to evaluate the antiarrhythmic effect of the pyrolysis products.Results:Two new diterpenoid alkaloids,pyroacetylaconitine and 16-epi-pyroacetylaconitine,a pair of epimers at C-16,were isolated.After comparing the structures of these compounds,possible transformation pathways were proposed.Compared with the prototype compound,3-acetylaconitine,the cardiotoxicity and acute toxicity of the heat-transformed products were significantly decreased.In the biological activity assay,the two pyrolysis products exhibited an effective increase in ventricular premature beat latency,a reduction in the occurrence of ventricular tachycardia,as well as an increase in the rate of arrhythmia inhibition,implying strong antiarrhythmic activity.Conclusion:Compared with 3-acetylaconitine,its pyrolysis products displayed lower toxicity and good antiarrhythmic effects;thus,they have potential for being developed into antiarrhythmic medicines.
基金Supported by the Science and Technique Plan of the Education Department of Yunnan Province:the Effect of the Ethanol Extract of Muxiang(Radix Aucklandiae)on Gastric Ulcer in Rats(No.2018JS285)Yunnan Innovation Team of Application Research on Tradtional Chinese Medicine Theory of Disease Prevention at Yunnan University of TCM(No.2017HC011)Yunnan Provincial University Key Laboratory of Aromatic Chinese Herb Research.
文摘OBJECTIVE:To investigate the effect of ethanol extracts of Muxiang(Radix Aucklandiae)(RA)on gastric ulcers in rats and explore the potential mechanisms.METHODS:A model was established by ethanol(0.75 m L/kg).According to body weight,rats were pretreated with RA extracts(2.5 or 5 g/kg).The rats were administered 95%ethanol orally after 1 h.The effects of ethanol were evaluated by measuring the gastric secretion volume,p H,pepsin activity,and ulcer area.Histological analysis and immunohistochemistry were also conducted.Furthermore,the effect of the ethanol extract of RA on transiting activity of the gastrointestinal tract was observed in mice.RESULTS:Intragastric administration of RA extracts protected the gastric mucosa from ethanol-induced gastric ulcers,while reducing submucosal edema and preventing hemorrhagic damage.Moreover,the extracts increased the production of gastric mucus,upregulated Bcl-2,and downregulated Bax expression.Importantly,pretreated rats exhibited no significant change in the gastric secretion volume,gastric juice acidity,or pepsin.Furthermore,pretreatment prominently(P<0.05)enhanced propulsive movement of the gastrointestinal tract in normal mice and mice with gastrointestinal motility disorders.CONCLUSION:Ethanol extracts of RA ameliorated gastric lesions in the gastric ulcer rat model.The mechanisms of action were related to improvement of gastrointestinal dynamics,maintenance of mucus integrity,and inhibition of apoptosis by downregulating proapoptotic Bax protein and upregulating anti-apoptotic Bcl-2 protein.
基金Supported by the National Natural Science Foundation of China(No.81503361)。
文摘Objective:To investigate the effect of seabuckthorn berries extract(SBE)on pulmonary vascular hyperpermeability in the mice model of acute lung injury(ALI)induced by lipopolysaccharide(LPS).Methods:Sixty Kunming mice were allocated into 6 groups by a random number table,including control,LPS,dexamethasone(Dex,1 mg/kg),and 120,240 and 480 mg/kg SBE groups,10 mice in each group.Except the control group,mice were pre-treated with Dex and SBE,respectively,for 7 days before LPS was intraperitoneally injected to induce ALl.Pulmonary vascular hyperpermeabilitywas evaluated by histopathologic observation and transvascular leakagedetermination.Tumor necrosis factor alpha(TNF-α)and interleukin-6(IL-6)levels in serum were measured using enzyme-linked immunosorbent assay.The expression of nuclear factor-kappa B(NF-k B)p65 in lung cells was determined by immunofluorescence analysis.The contents of cytoplasmic inhibitorof nuclear factor-kBkinase(IKK)and nuclearp65,as wellasdownstreamproteins of E-selectin(CD62E)and intercellular adhesion molecule-1(ICAM-1),were determined using Western blot analysis.Results:Histopathological observation confirmed SBEtreatment alleviatedmorphological lesion induced by LPS.Compared with the LPS group,480 mg/kg SBE significantly decreased the water content of lung,Evans blue accumulation in lung tissue,and protein concentration and neutrophils count in bronchoalveolar lavage fluid(P<o.01);moreover,480 mg/kg SBE significantly suppressed release of TNF-αand IL-6,and down-regulated expressions of IKK,nuclear p65,ICAM-1 and CD62E(P<0.01).Conclusion:SBE maintained alveolar-capillary barrier integrity under endotoxin challenge in mice by suppressing the key factors in the pathogenesis of ALl.
