A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration...A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.展开更多
Rice-based flakes made from 7 mid-season rice varieties （4 indica and 3 japonica subspecies） and 3 early season indica varieties produced both in early and late season were studied for their main physicochemical cha...Rice-based flakes made from 7 mid-season rice varieties （4 indica and 3 japonica subspecies） and 3 early season indica varieties produced both in early and late season were studied for their main physicochemical characters, including water activity, film thickness, bulk density （BD）, ash, reducing sugars （RS）, protein, phytic acid, starch, amylose, and rapid visco analyser （RVA） viscosity profile. A significant varietal effect was found for all these parameters （P〈 0.05 or 0.01） except starch. Strong variety x season interactions were observed but the seasonal effect was less pronounced, and only significant （P〈0.01） for RS. The apparent amylose content （AAC） office flakes was significantly correlated （P〈0.01） with AAC （r=0.99） and several RVA profile parameters [e.g., final viscosity （r=0.92）] of rice flour. For rice flakes, BD was significantly correlated with AAC （r=0.82, P〈0.01） and most RVA profile parameters. The study indicates that varietal and environmental effects should be considered simultaneously for optimal production of rice grains for rice flakes manufacturing.展开更多
文摘A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.
文摘Rice-based flakes made from 7 mid-season rice varieties （4 indica and 3 japonica subspecies） and 3 early season indica varieties produced both in early and late season were studied for their main physicochemical characters, including water activity, film thickness, bulk density （BD）, ash, reducing sugars （RS）, protein, phytic acid, starch, amylose, and rapid visco analyser （RVA） viscosity profile. A significant varietal effect was found for all these parameters （P〈 0.05 or 0.01） except starch. Strong variety x season interactions were observed but the seasonal effect was less pronounced, and only significant （P〈0.01） for RS. The apparent amylose content （AAC） office flakes was significantly correlated （P〈0.01） with AAC （r=0.99） and several RVA profile parameters [e.g., final viscosity （r=0.92）] of rice flour. For rice flakes, BD was significantly correlated with AAC （r=0.82, P〈0.01） and most RVA profile parameters. The study indicates that varietal and environmental effects should be considered simultaneously for optimal production of rice grains for rice flakes manufacturing.
