In this study,an innovative technique is introduced to significantly enhance the sensitivity of electronic speckle pattern interferometry(ESPI)for the dynamic assessment of specular(mirrorlike)object deformations.By u...In this study,an innovative technique is introduced to significantly enhance the sensitivity of electronic speckle pattern interferometry(ESPI)for the dynamic assessment of specular(mirrorlike)object deformations.By utilizing a common-path illumination strategy,wherein illumination and observation beams are precisely aligned,this method effectively doubles the optical path difference,leading to a twofold increase in measurement sensitivity.In addition,this method mitigates the effects of speckle noise on the measurement of minor deformations,expanding the applications of ESPI.Theoretical and experimental evaluations corroborate the efficacy of this approach.展开更多
Circulating tumor cells(CTCs)is an established biomarker of cancer metastasis.The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination.Although studies have r...Circulating tumor cells(CTCs)is an established biomarker of cancer metastasis.The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination.Although studies have revealed that the circadian rhythm may disrupt the growth of tumors,it is generally unclear whether the circadian rhythm controls the release of CTCs.In clinical examinations,the current in vitro methods for detecting CTCs in blood samples are based on a fundamental assumption that CTC counts in the peripheral blood do not change significantly over time,which is being challenged by recent studies.Since it is not practical to draw blood from patients repeatedly,a feasible strategy to investigate the circadian rhythm of CTCs is to monitor them by in vivo detection methods.Fluoresce nee in vivo flow cytometry(IVFC)is a powerful optical technique that is able to detect fluoresce nt circulating cells directly in living animals in a noninvasive manner over a long period of time.In this study,we applied fluorescence IVFC to monitor CTCs noninvasively in an orthotopic mouse model of human prostate cancer.We observed that CTCs exhibited stochastic bursts over cancer progression.The probability of the bursting activity was higher at early stages than at late stages.We longitudinally monitored CTCs over a 24-h period,and our results revealed striking daily oscillations in CTC counts that peaked at the onset of the night(active phase for rodents),suggesting that the release of CTCs might be regulated by the circadian rhythm.展开更多
Dear Editor,We thank Niedre et al.for their correspondence regarding our recent paper1.They proposed a point that the temporal distribution of circulating tumor cells(CTCs)monitored by diffuse in vivo flow cytometry i...Dear Editor,We thank Niedre et al.for their correspondence regarding our recent paper1.They proposed a point that the temporal distribution of circulating tumor cells(CTCs)monitored by diffuse in vivo flow cytometry in a multiple myeloma mouse model in their previous work2 might be different from our results.Niedre et al.claimed that CTC detection statistics deviated from Poisson but did not found circadian variations in CTC numbers in a multiple myeloma mouse model.They also cite another literature by Juratli.et al.3,in which the authors reported that CTC numbers did not always correlate with tumor size during cancer progression.However,by establishing an orthotopic mouse model of prostate cancer and utilizing the technology in vivo flow cytometry(IVFC),we found CTCs exhibited bursting activity and daily oscillation in an orthotopic model of prostate cancer.展开更多
A dual-wavelength erbium-doped fiber laser(EDFL) with outstanding stability is presented. In the fiber laser system, two nested active optical fiber ring filters are configured to improve the comb spectrum performance...A dual-wavelength erbium-doped fiber laser(EDFL) with outstanding stability is presented. In the fiber laser system, two nested active optical fiber ring filters are configured to improve the comb spectrum performance, a saturable absorber is employed to form a gain grating for both filtering and frequency stabilizing, two cascaded fiber Bragg gratings(FBGs) are utilized to achieve dual-wavelength output, and a variable attenuator is arranged to adjust output power. Experimental results illustrate that the peak wavelength drift is less than 3 pm, and a good linear relationship between output power and pump power is realized.展开更多
A fiber laser micro-nano sensor based on colloidal crystal structure is proposed in this paper.The fiber laser has stable frequency and narrow linewidth.It is realized by using an unpumped erbium-doped fiber(EDF)as th...A fiber laser micro-nano sensor based on colloidal crystal structure is proposed in this paper.The fiber laser has stable frequency and narrow linewidth.It is realized by using an unpumped erbium-doped fiber(EDF)as the saturable absorber.The saturable absorber possesses the shape of taper.The laser threshold can be effectively reduced by the tapered saturable absorber.The tapered fiber coated with colloidal crystal as sensing unit is studied.The concentration of ethanol can be obtained from the detection of the output laser wavelength.It can be extensively used in chemical,medical and biological detections.展开更多
We investigate the mechanisms to realize the Raman laser switching in a silica rod microresonator with mode-interactionassisted excitation.The laser switching can be triggered between two whispering gallery modes[WGMs...We investigate the mechanisms to realize the Raman laser switching in a silica rod microresonator with mode-interactionassisted excitation.The laser switching can be triggered between two whispering gallery modes[WGMs]with either the same or distinct mode families,depending on the pumping conditions.The experimental observations are in excellent agreement with a theoretical analysis based on coupled-mode equations with intermodal interaction terms involved.Additionally,we also demonstrate switching of a single-mode Raman laser and a wideband spectral tuning range up to~32.67 nm by selective excitation of distinct mode sequences.The results contribute to the understanding of Raman lasing formation dynamics via interaction with transverse mode sequences and may extend the microcavity-based Raman microlasers to potential areas in switchable light sources,optical memories,and high sensitivity sensors.展开更多
The advantage of spatial phase-shifting shearography is its ability to extract the phase from a single speckle pattern;however,it often faces spectrum overlapping,which seriously affects phase quality.In this paper,we...The advantage of spatial phase-shifting shearography is its ability to extract the phase from a single speckle pattern;however,it often faces spectrum overlapping,which seriously affects phase quality.In this paper,we propose a shearography phase-extraction method based on windowed Fourier ridges,which can effectively extract phase information even in the presence of severe spectrum overlapping.A simple and efficient method was applied to determine the parameters of the windowed Fourier ridges,and a linear variation window was used to match the phase-extraction requirements for different frequency coordinates.A numerical simulation was quantitatively conducted to compare the phase-extraction results of the proposed method with those of the conventional method for various cases,and a shearography system was built with two types of objects to demonstrate the feasibility of the proposed method.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52375536 and 52375535)the Key Research and Development Program of Jiangxi Province(No.20223BBE51010)。
文摘In this study,an innovative technique is introduced to significantly enhance the sensitivity of electronic speckle pattern interferometry(ESPI)for the dynamic assessment of specular(mirrorlike)object deformations.By utilizing a common-path illumination strategy,wherein illumination and observation beams are precisely aligned,this method effectively doubles the optical path difference,leading to a twofold increase in measurement sensitivity.In addition,this method mitigates the effects of speckle noise on the measurement of minor deformations,expanding the applications of ESPI.Theoretical and experimental evaluations corroborate the efficacy of this approach.
基金the National Key Research and Development Program of China(Grant No.2019YFC1604604)the National Science Fund for Distinguished Young Scholars(Grant No.61425006)+2 种基金the SJTU Medicine Engineering Interdisciplinary Research Fund(Grant No.YG2017MS19)the Program of Shanghai Technology Research Leader(Grant No.17XD1402200)the Fundamental Research Funds for the Central Universities,and the National Natural Science Foundation of China(Grant No.81661168014,61975118 and 62075013).
文摘Circulating tumor cells(CTCs)is an established biomarker of cancer metastasis.The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination.Although studies have revealed that the circadian rhythm may disrupt the growth of tumors,it is generally unclear whether the circadian rhythm controls the release of CTCs.In clinical examinations,the current in vitro methods for detecting CTCs in blood samples are based on a fundamental assumption that CTC counts in the peripheral blood do not change significantly over time,which is being challenged by recent studies.Since it is not practical to draw blood from patients repeatedly,a feasible strategy to investigate the circadian rhythm of CTCs is to monitor them by in vivo detection methods.Fluoresce nee in vivo flow cytometry(IVFC)is a powerful optical technique that is able to detect fluoresce nt circulating cells directly in living animals in a noninvasive manner over a long period of time.In this study,we applied fluorescence IVFC to monitor CTCs noninvasively in an orthotopic mouse model of human prostate cancer.We observed that CTCs exhibited stochastic bursts over cancer progression.The probability of the bursting activity was higher at early stages than at late stages.We longitudinally monitored CTCs over a 24-h period,and our results revealed striking daily oscillations in CTC counts that peaked at the onset of the night(active phase for rodents),suggesting that the release of CTCs might be regulated by the circadian rhythm.
文摘Dear Editor,We thank Niedre et al.for their correspondence regarding our recent paper1.They proposed a point that the temporal distribution of circulating tumor cells(CTCs)monitored by diffuse in vivo flow cytometry in a multiple myeloma mouse model in their previous work2 might be different from our results.Niedre et al.claimed that CTC detection statistics deviated from Poisson but did not found circadian variations in CTC numbers in a multiple myeloma mouse model.They also cite another literature by Juratli.et al.3,in which the authors reported that CTC numbers did not always correlate with tumor size during cancer progression.However,by establishing an orthotopic mouse model of prostate cancer and utilizing the technology in vivo flow cytometry(IVFC),we found CTCs exhibited bursting activity and daily oscillation in an orthotopic model of prostate cancer.
基金supported by the Transformation and Industrialization of Scientific and Technological Achievements(Nos.PXM2013_014224_000077 and PXM2012_014224_000019)the Open Foundation of Beijing Engineering Research Center for Optoelectronic Information and Instrument(No.GD20130006)
文摘A dual-wavelength erbium-doped fiber laser(EDFL) with outstanding stability is presented. In the fiber laser system, two nested active optical fiber ring filters are configured to improve the comb spectrum performance, a saturable absorber is employed to form a gain grating for both filtering and frequency stabilizing, two cascaded fiber Bragg gratings(FBGs) are utilized to achieve dual-wavelength output, and a variable attenuator is arranged to adjust output power. Experimental results illustrate that the peak wavelength drift is less than 3 pm, and a good linear relationship between output power and pump power is realized.
基金the Transformation and Industrialization of Scientific and Technological Achievements(Nos.PXM2013_014224_000077 and PXM2012_014224_000019)the School Fund of Beijing Information Science&Technology University(No.1225006)the Open Foundation of Beijing Engineering Research Center for Optoelectronic Information and Instrument(No.GD20120010)
文摘A fiber laser micro-nano sensor based on colloidal crystal structure is proposed in this paper.The fiber laser has stable frequency and narrow linewidth.It is realized by using an unpumped erbium-doped fiber(EDF)as the saturable absorber.The saturable absorber possesses the shape of taper.The laser threshold can be effectively reduced by the tapered saturable absorber.The tapered fiber coated with colloidal crystal as sensing unit is studied.The concentration of ethanol can be obtained from the detection of the output laser wavelength.It can be extensively used in chemical,medical and biological detections.
基金supported by the National Natural Science Foundation of China(No.62005071)the Natural Science Foundation of Anhui Province(No.2008085QF312)+1 种基金the Fundamental Research Funds for the Central Universities(No.JZ2021HGTB0079)the National Key Research and Development Program of China(No.2019YFE010747)。
文摘We investigate the mechanisms to realize the Raman laser switching in a silica rod microresonator with mode-interactionassisted excitation.The laser switching can be triggered between two whispering gallery modes[WGMs]with either the same or distinct mode families,depending on the pumping conditions.The experimental observations are in excellent agreement with a theoretical analysis based on coupled-mode equations with intermodal interaction terms involved.Additionally,we also demonstrate switching of a single-mode Raman laser and a wideband spectral tuning range up to~32.67 nm by selective excitation of distinct mode sequences.The results contribute to the understanding of Raman lasing formation dynamics via interaction with transverse mode sequences and may extend the microcavity-based Raman microlasers to potential areas in switchable light sources,optical memories,and high sensitivity sensors.
基金supported by the National Key Research and Development Program of China(No.2016YFF0101803)Hefei Municipal Natural Science Foundation(No.2021017).
文摘The advantage of spatial phase-shifting shearography is its ability to extract the phase from a single speckle pattern;however,it often faces spectrum overlapping,which seriously affects phase quality.In this paper,we propose a shearography phase-extraction method based on windowed Fourier ridges,which can effectively extract phase information even in the presence of severe spectrum overlapping.A simple and efficient method was applied to determine the parameters of the windowed Fourier ridges,and a linear variation window was used to match the phase-extraction requirements for different frequency coordinates.A numerical simulation was quantitatively conducted to compare the phase-extraction results of the proposed method with those of the conventional method for various cases,and a shearography system was built with two types of objects to demonstrate the feasibility of the proposed method.
