Objective:To investigate the effect of Oroxylum indicum fruit extract on high-fat diet-induced hyperlipidemic mice.Methods:The phytochemical composition of Oroxylum indicum fruit extract was determined by liquid chrom...Objective:To investigate the effect of Oroxylum indicum fruit extract on high-fat diet-induced hyperlipidemic mice.Methods:The phytochemical composition of Oroxylum indicum fruit extract was determined by liquid chromatographymass spectrometry/mass spectrometry(LC-MS/MS)and gas chromatography-mass spectrometry.Forty-two male mice were used.The mice were divided into six groups:normal control,high-fat diet control,simvastatin treatment(20 mg/kg BW/day),and Oroxylum indicum fruit extract(100,200,300 mg/kg BW/day)treatment groups.Food intake,body weight,serum parameters,lipid profile,and histopathological lesions of the kidney,liver,and epididymal fat were observed.Results:LC-MS/MS results revealed four major components of Oroxylum indicum fruit extract:luteolin,apigenin,baicalein,and oroxylin A.Twenty-seven volatile oils were identified from Oroxylum indicum fruit extract.Daily oral administration of Oroxylum indicum fruit extract at 100 to 300 mg/kg BW/day significantly reduced the body weight,total cholesterol,triglyceride,and low-density lipoprotein cholesterol level(P<0.05),whereas high-density lipoprotein cholesterol was higher than the high-fat diet control group.Treatment with 300 mg/kg BW/day Oroxylum indicum fruit extract reduced the pathological lesion and prevented fat accumulation in the kidney and liver.Conclusions:Oroxylum indicum fruit extract has hypolipidemic effect in hyperlipidemic mice,and the active ingredients of Oroxylum indicum fruit extract,both flavonoids and volatile oils,should be further explored as an antihyperlipidemic agent.展开更多
Objective:To determine the prevalence of mosquito larvae in campus areas and the infection rate of endosymbiotic bacteria,Wolbachia in mosquito larvae.Method:The mosquito larvae samples were collected in residential a...Objective:To determine the prevalence of mosquito larvae in campus areas and the infection rate of endosymbiotic bacteria,Wolbachia in mosquito larvae.Method:The mosquito larvae samples were collected in residential areas and academic buildings of Suranaree University of Technology located in Northeastern Thailand during 2017-2018.Mosquito species identification was performed using GLOBE mosquito protocols and Rattanarithikul&Panthusiri’s keys.The gene encoding for the surface protein of Wolbachia was amplified by PCR and confirmed by DNA sequencing.Results:Armigeres sp.is the highest proportion of mosquito larvae followed by Culex spp.,Aedes albopictus,Aedes aegypti,and Toxorynchites spp.,respectively.Aedes aegypti have breeding sites mostly in the containers found indoors,whereas the main breeding sites of Aedes albopictus were found in both outdoors and indoors.The House Index and Breteau Index for Aedes spp.was more than 5%and 20%,respectively,in both areas,indicating that these areas are dengue sensitive.The highest proportion of Wolbachia infection was found in the larvae of Culex spp.(86.21%),followed by Aedes albopictus(69.23%)and rarely detected in Aedes aegypti(9.09%).Conclusion:The present study reported the first natural infection of Wolbachia in mosquito larvae in Thailand.Our result suggested that the mosquito species containing higher proportion of Wolbachia are less likely to be vectors for dengue.Therefore,Wolbachia transfection in mosquito larvae could be applied as a biocontrol for dengue and other mosquito-borne disease prevention.展开更多
Objective:To investigate the effect of Heliotropium indicum L.(H.indicum L.)on uterine involution and its underlying mechanisms in both in vivo and in vitro study.Methods:For in vivo studies,postpartum rats were rando...Objective:To investigate the effect of Heliotropium indicum L.(H.indicum L.)on uterine involution and its underlying mechanisms in both in vivo and in vitro study.Methods:For in vivo studies,postpartum rats were randomly divided into 2 groups(n=24 for each):control group and treated group which were orally and daily administered with ethanolic extract of H.indicum L.(250 mg/kg body weight)until day 5 of postpartum.Uteri were collected for analysis of weight,cross-sectional area,collagen cross-sectional area,and collagen content on postpartum day 1,3,and 5(n=8 for each)from both groups.Blood samples were collected for hepatotoxicity and 17?-estradiol(E_(2))measurement.For in vitro studies,the extract effects on uterine contraction at half maximum effective concentration of 2.50 mg/mL were studied in organ bath system for at least 20 min.Results:Uterine parameters were significantly decreased after treated with extract of H.indicum L.(P<0.05).H.indicum L.extract significantly accelerated the reduction of those parameters and significantly decreased E_(2)(P<0.05).The extract facilitated uterine involution with no hepatotoxicity.H.indicum L.extract significantly stimulated uterine contraction(P<0.05)and synergized with oxytocin,prostaglandin and its precursor,linoleic acid.By investigating the different sequencing of the extract with the additional stimulants(added before or after),the two showed antagonistic effects,but still showed potentiated force when compared with control(without the stimulants).Conclusions:The underlying mechanisms by which H.indicum L.facilitated uterine involution might be due to reducing E_(2)which induces collagenase activity,leading to decreases in uterine weight and size and stimulating uterine contraction.Our study provides new findings for future drug development for facilitating uterine involution with H.indicum L.展开更多
文摘Objective:To investigate the effect of Oroxylum indicum fruit extract on high-fat diet-induced hyperlipidemic mice.Methods:The phytochemical composition of Oroxylum indicum fruit extract was determined by liquid chromatographymass spectrometry/mass spectrometry(LC-MS/MS)and gas chromatography-mass spectrometry.Forty-two male mice were used.The mice were divided into six groups:normal control,high-fat diet control,simvastatin treatment(20 mg/kg BW/day),and Oroxylum indicum fruit extract(100,200,300 mg/kg BW/day)treatment groups.Food intake,body weight,serum parameters,lipid profile,and histopathological lesions of the kidney,liver,and epididymal fat were observed.Results:LC-MS/MS results revealed four major components of Oroxylum indicum fruit extract:luteolin,apigenin,baicalein,and oroxylin A.Twenty-seven volatile oils were identified from Oroxylum indicum fruit extract.Daily oral administration of Oroxylum indicum fruit extract at 100 to 300 mg/kg BW/day significantly reduced the body weight,total cholesterol,triglyceride,and low-density lipoprotein cholesterol level(P<0.05),whereas high-density lipoprotein cholesterol was higher than the high-fat diet control group.Treatment with 300 mg/kg BW/day Oroxylum indicum fruit extract reduced the pathological lesion and prevented fat accumulation in the kidney and liver.Conclusions:Oroxylum indicum fruit extract has hypolipidemic effect in hyperlipidemic mice,and the active ingredients of Oroxylum indicum fruit extract,both flavonoids and volatile oils,should be further explored as an antihyperlipidemic agent.
文摘Objective:To determine the prevalence of mosquito larvae in campus areas and the infection rate of endosymbiotic bacteria,Wolbachia in mosquito larvae.Method:The mosquito larvae samples were collected in residential areas and academic buildings of Suranaree University of Technology located in Northeastern Thailand during 2017-2018.Mosquito species identification was performed using GLOBE mosquito protocols and Rattanarithikul&Panthusiri’s keys.The gene encoding for the surface protein of Wolbachia was amplified by PCR and confirmed by DNA sequencing.Results:Armigeres sp.is the highest proportion of mosquito larvae followed by Culex spp.,Aedes albopictus,Aedes aegypti,and Toxorynchites spp.,respectively.Aedes aegypti have breeding sites mostly in the containers found indoors,whereas the main breeding sites of Aedes albopictus were found in both outdoors and indoors.The House Index and Breteau Index for Aedes spp.was more than 5%and 20%,respectively,in both areas,indicating that these areas are dengue sensitive.The highest proportion of Wolbachia infection was found in the larvae of Culex spp.(86.21%),followed by Aedes albopictus(69.23%)and rarely detected in Aedes aegypti(9.09%).Conclusion:The present study reported the first natural infection of Wolbachia in mosquito larvae in Thailand.Our result suggested that the mosquito species containing higher proportion of Wolbachia are less likely to be vectors for dengue.Therefore,Wolbachia transfection in mosquito larvae could be applied as a biocontrol for dengue and other mosquito-borne disease prevention.
基金Supported by Suranaree University of Technology(Scholarship No.SUT-Kitti Pundit-1-2559-M5910311,Grant No.SUT1-104-55-12-21)。
文摘Objective:To investigate the effect of Heliotropium indicum L.(H.indicum L.)on uterine involution and its underlying mechanisms in both in vivo and in vitro study.Methods:For in vivo studies,postpartum rats were randomly divided into 2 groups(n=24 for each):control group and treated group which were orally and daily administered with ethanolic extract of H.indicum L.(250 mg/kg body weight)until day 5 of postpartum.Uteri were collected for analysis of weight,cross-sectional area,collagen cross-sectional area,and collagen content on postpartum day 1,3,and 5(n=8 for each)from both groups.Blood samples were collected for hepatotoxicity and 17?-estradiol(E_(2))measurement.For in vitro studies,the extract effects on uterine contraction at half maximum effective concentration of 2.50 mg/mL were studied in organ bath system for at least 20 min.Results:Uterine parameters were significantly decreased after treated with extract of H.indicum L.(P<0.05).H.indicum L.extract significantly accelerated the reduction of those parameters and significantly decreased E_(2)(P<0.05).The extract facilitated uterine involution with no hepatotoxicity.H.indicum L.extract significantly stimulated uterine contraction(P<0.05)and synergized with oxytocin,prostaglandin and its precursor,linoleic acid.By investigating the different sequencing of the extract with the additional stimulants(added before or after),the two showed antagonistic effects,but still showed potentiated force when compared with control(without the stimulants).Conclusions:The underlying mechanisms by which H.indicum L.facilitated uterine involution might be due to reducing E_(2)which induces collagenase activity,leading to decreases in uterine weight and size and stimulating uterine contraction.Our study provides new findings for future drug development for facilitating uterine involution with H.indicum L.
